CA2175590A1 - Method of producing recombinant dimeric enzyme - Google Patents

Method of producing recombinant dimeric enzyme

Info

Publication number
CA2175590A1
CA2175590A1 CA 2175590 CA2175590A CA2175590A1 CA 2175590 A1 CA2175590 A1 CA 2175590A1 CA 2175590 CA2175590 CA 2175590 CA 2175590 A CA2175590 A CA 2175590A CA 2175590 A1 CA2175590 A1 CA 2175590A1
Authority
CA
Canada
Prior art keywords
enzyme
dna
leu
subunit
prokaryotic host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2175590
Other languages
English (en)
French (fr)
Inventor
Robin J. Ziegler
Susan Long
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2175590A1 publication Critical patent/CA2175590A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA 2175590 1993-11-02 1994-11-02 Method of producing recombinant dimeric enzyme Abandoned CA2175590A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14665593A 1993-11-02 1993-11-02
US08/146,655 1993-11-02

Publications (1)

Publication Number Publication Date
CA2175590A1 true CA2175590A1 (en) 1995-05-11

Family

ID=22518370

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2175590 Abandoned CA2175590A1 (en) 1993-11-02 1994-11-02 Method of producing recombinant dimeric enzyme

Country Status (5)

Country Link
EP (1) EP0729505A1 (ja)
JP (1) JPH09504698A (ja)
AU (1) AU8131994A (ja)
CA (1) CA2175590A1 (ja)
WO (1) WO1995012662A1 (ja)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6174715B1 (en) * 1996-06-14 2001-01-16 Toyota Jidosha Kabushiki Kaisha Prenyl diphosphate synthetase genes
US5981249A (en) * 1998-02-05 1999-11-09 Spectral Diagnostics, Inc. Single-chain polypeptides comprising creatine kinase M and creatine kinase B
JP2001299361A (ja) * 2000-04-28 2001-10-30 Oriental Yeast Co Ltd 溶液安定性の組換えヒトクレアチンキナーゼヘテロダイマー
GB0203552D0 (en) * 2002-02-14 2002-04-03 Beaumont Nicholas J Peptide growth factor
DE102004013843A1 (de) * 2004-03-20 2005-10-13 Degussa Ag Expression von Nitrilhydratasen im Zwei-Vektor-Expressionssystem

Also Published As

Publication number Publication date
EP0729505A1 (en) 1996-09-04
AU8131994A (en) 1995-05-23
WO1995012662A1 (en) 1995-05-11
JPH09504698A (ja) 1997-05-13

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Legal Events

Date Code Title Description
FZDE Dead