CA2173793A1 - Fermentation du pentose par zymomonas recombinant - Google Patents
Fermentation du pentose par zymomonas recombinantInfo
- Publication number
- CA2173793A1 CA2173793A1 CA002173793A CA2173793A CA2173793A1 CA 2173793 A1 CA2173793 A1 CA 2173793A1 CA 002173793 A CA002173793 A CA 002173793A CA 2173793 A CA2173793 A CA 2173793A CA 2173793 A1 CA2173793 A1 CA 2173793A1
- Authority
- CA
- Canada
- Prior art keywords
- microorganism
- transketolase
- transaldolase
- gene
- exogenous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000002972 pentoses Chemical class 0.000 title claims abstract 14
- 238000000855 fermentation Methods 0.000 title claims abstract 4
- 230000004151 fermentation Effects 0.000 title claims abstract 4
- 241000588901 Zymomonas Species 0.000 title claims 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract 37
- 244000005700 microbiome Species 0.000 claims abstract 32
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract 26
- 102100028601 Transaldolase Human genes 0.000 claims abstract 15
- 108020004530 Transaldolase Proteins 0.000 claims abstract 15
- 102000014701 Transketolase Human genes 0.000 claims abstract 15
- 108010043652 Transketolase Proteins 0.000 claims abstract 15
- 241000588902 Zymomonas mobilis Species 0.000 claims abstract 14
- 108010018080 L-arabinose isomerase Proteins 0.000 claims abstract 10
- 108090000416 L-ribulose-5-phosphate 4-epimerases Proteins 0.000 claims abstract 10
- 108020002667 ribulokinase Proteins 0.000 claims abstract 10
- 108700040099 Xylose isomerases Proteins 0.000 claims abstract 5
- 241000588724 Escherichia coli Species 0.000 claims abstract 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract 4
- 102100029089 Xylulose kinase Human genes 0.000 claims abstract 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract 4
- 229920002678 cellulose Polymers 0.000 claims abstract 4
- 239000001913 cellulose Substances 0.000 claims abstract 4
- 239000008103 glucose Substances 0.000 claims abstract 4
- 108091022915 xylulokinase Proteins 0.000 claims abstract 4
- 238000000034 method Methods 0.000 claims 17
- 102000004190 Enzymes Human genes 0.000 claims 9
- 108090000790 Enzymes Proteins 0.000 claims 9
- 229940088598 enzyme Drugs 0.000 claims 9
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 7
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 7
- SRBFZHDQGSBBOR-IOVATXLUSA-N Xylose Natural products O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims 5
- 108010059892 Cellulase Proteins 0.000 claims 3
- 230000003301 hydrolyzing effect Effects 0.000 claims 3
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 claims 2
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 claims 2
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims 2
- 125000000089 arabinosyl group Chemical group C1([C@@H](O)[C@H](O)[C@H](O)CO1)* 0.000 claims 2
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims 2
- 239000013612 plasmid Substances 0.000 claims 1
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 claims 1
- 229920002488 Hemicellulose Polymers 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/422,424 US5712133A (en) | 1994-04-15 | 1995-04-14 | Pentose fermentation by recombinant zymomonas |
| US08/421,996 US5726053A (en) | 1994-04-15 | 1995-04-14 | Recombinant Zymomonas for pentose fermentation |
| US08/421,996 | 1995-04-14 | ||
| US08/422,424 | 1995-04-14 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2173793A1 true CA2173793A1 (fr) | 1996-10-15 |
| CA2173793C CA2173793C (fr) | 2011-01-11 |
Family
ID=27025447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2173793A Expired - Lifetime CA2173793C (fr) | 1995-04-14 | 1996-04-10 | Fermentation du pentose par zymomonas recombinant |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR100455096B1 (fr) |
| AU (1) | AU698662B2 (fr) |
| CA (1) | CA2173793C (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU774414B2 (en) * | 1997-05-06 | 2004-06-24 | Midwest Research Institute | Single (zymomonas mobilis) strain for xylose and arabinose fermentation |
-
1996
- 1996-04-10 CA CA2173793A patent/CA2173793C/fr not_active Expired - Lifetime
- 1996-04-11 AU AU50582/96A patent/AU698662B2/en not_active Expired
- 1996-04-13 KR KR1019960011100A patent/KR100455096B1/ko not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| KR100455096B1 (ko) | 2005-01-13 |
| KR960037834A (ko) | 1996-11-19 |
| CA2173793C (fr) | 2011-01-11 |
| AU5058296A (en) | 1996-10-24 |
| AU698662B2 (en) | 1998-11-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20160411 |