CA2164320A1 - Detection de sequences d'acide nucleique - Google Patents

Detection de sequences d'acide nucleique

Info

Publication number
CA2164320A1
CA2164320A1 CA002164320A CA2164320A CA2164320A1 CA 2164320 A1 CA2164320 A1 CA 2164320A1 CA 002164320 A CA002164320 A CA 002164320A CA 2164320 A CA2164320 A CA 2164320A CA 2164320 A1 CA2164320 A1 CA 2164320A1
Authority
CA
Canada
Prior art keywords
rna
sequence
dna
promoter
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002164320A
Other languages
English (en)
Inventor
Asher Nathan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Intelligene Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2164320A1 publication Critical patent/CA2164320A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de détection de la présence d'une séquence d'acide nucléique recherchée dans un échantillon, comportant essentiellement deux étapes. Dans une première étape, on fait réagir l'échantillon d'une manière donnant lieu à la production d'un ARN de déclenchement, l'échantillon contenant la séquence recherchée. Dans la seconde étape, on fait incuber le produit de réaction dans des conditions appropriées, à l'aide d'un ensemble de réactifs d'amplification, de sorte que l'on obtient une grande quantité d'un produit d'acide nucléique en présence de l'ARN de déclenchement. La détection de ce produit indique par conséquent la présence de la séquence recherchée dans l'échantillon.
CA002164320A 1993-06-03 1994-06-02 Detection de sequences d'acide nucleique Abandoned CA2164320A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL10589493A IL105894A (en) 1993-06-03 1993-06-03 Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
IL105894 1993-06-03

Publications (1)

Publication Number Publication Date
CA2164320A1 true CA2164320A1 (fr) 1994-12-22

Family

ID=11064903

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002164320A Abandoned CA2164320A1 (fr) 1993-06-03 1994-06-02 Detection de sequences d'acide nucleique

Country Status (5)

Country Link
EP (1) EP0710292A4 (fr)
AU (1) AU7047794A (fr)
CA (1) CA2164320A1 (fr)
IL (1) IL105894A (fr)
WO (1) WO1994029481A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US6214546B1 (en) 1995-02-27 2001-04-10 Intelligene Ltd. Detection of biomolecules
EP0822992A4 (fr) * 1995-02-27 2000-08-09 Intelligene Ltd Detection de biomolecules
AU728342B2 (en) * 1997-02-21 2001-01-04 Johnson & Johnson Research Pty. Limited Selective ligation and amplification method
AUPO524897A0 (en) 1997-02-21 1997-03-20 Johnson & Johnson Research Pty. Limited Method of amplifying specific nucleic acid target sequences
AU747140B2 (en) 1998-01-27 2002-05-09 British Biocell International Limited Method for detection of nucleic acid target sequences involving (in vitro) transcription from an RNA promoter
IL138126A0 (en) 1998-03-05 2001-10-31 Johnson & Johnson Res Pty Ltd Zymogenic nucleic acid detection methods and related molecules and kits
IL130606A0 (en) * 1999-06-23 2000-06-01 Intelligene Ltd An array of nucleic acid sequence
US7517870B2 (en) 2004-12-03 2009-04-14 Fondazione Telethon Use of compounds that interfere with the hedgehog signaling pathway for the manufacture of a medicament for preventing, inhibiting, and/or reversing ocular diseases related with ocular neovascularization

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO904633L (no) * 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
DE4132133A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen herstellung von ribonukleinsaeuren

Also Published As

Publication number Publication date
EP0710292A1 (fr) 1996-05-08
IL105894A (en) 1997-02-18
IL105894A0 (en) 1993-10-20
EP0710292A4 (fr) 1999-05-19
WO1994029481A1 (fr) 1994-12-22
AU7047794A (en) 1995-01-03

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued