CA2108564A1 - Cassette d'expression genique contenant une sequence non codante du gene de l'hormone de croissance - Google Patents
Cassette d'expression genique contenant une sequence non codante du gene de l'hormone de croissanceInfo
- Publication number
- CA2108564A1 CA2108564A1 CA002108564A CA2108564A CA2108564A1 CA 2108564 A1 CA2108564 A1 CA 2108564A1 CA 002108564 A CA002108564 A CA 002108564A CA 2108564 A CA2108564 A CA 2108564A CA 2108564 A1 CA2108564 A1 CA 2108564A1
- Authority
- CA
- Canada
- Prior art keywords
- gene
- sequence
- expression cassette
- growth hormone
- exon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 90
- 230000014509 gene expression Effects 0.000 title claims abstract description 42
- 108091026890 Coding region Proteins 0.000 title claims abstract description 21
- 108010051696 Growth Hormone Proteins 0.000 title claims abstract description 13
- 239000000122 growth hormone Substances 0.000 title claims abstract description 13
- 102000018997 Growth Hormone Human genes 0.000 title claims abstract 5
- 230000002068 genetic effect Effects 0.000 claims abstract description 12
- 239000002299 complementary DNA Substances 0.000 claims abstract description 11
- 230000001939 inductive effect Effects 0.000 claims abstract description 8
- 210000004102 animal cell Anatomy 0.000 claims abstract description 6
- 101001027952 Ovis aries Metallothionein-1A Proteins 0.000 claims description 14
- 241001465754 Metazoa Species 0.000 claims description 11
- 102000004169 proteins and genes Human genes 0.000 claims description 11
- 230000001580 bacterial effect Effects 0.000 claims description 9
- 101150111114 cysE gene Proteins 0.000 claims description 8
- 230000009261 transgenic effect Effects 0.000 claims description 8
- 239000002773 nucleotide Substances 0.000 claims description 7
- 125000003729 nucleotide group Chemical group 0.000 claims description 7
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 101150036393 aceB gene Proteins 0.000 claims description 5
- 101100350224 Bacillus subtilis (strain 168) pdhB gene Proteins 0.000 claims description 4
- 101100236536 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) glcB gene Proteins 0.000 claims description 4
- 101100406344 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) aceF gene Proteins 0.000 claims description 4
- 230000000749 insecticidal effect Effects 0.000 claims description 4
- 102000012286 Chitinases Human genes 0.000 claims description 3
- 108010022172 Chitinases Proteins 0.000 claims description 3
- 101100498063 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) cysB gene Proteins 0.000 claims description 3
- 101150094017 aceA gene Proteins 0.000 claims description 3
- 101150094831 cysK gene Proteins 0.000 claims description 3
- 101150112941 cysK1 gene Proteins 0.000 claims description 3
- 101150029709 cysM gene Proteins 0.000 claims description 3
- 101100242035 Bacillus subtilis (strain 168) pdhA gene Proteins 0.000 claims description 2
- 241000283690 Bos taurus Species 0.000 claims description 2
- 101100123255 Komagataeibacter xylinus aceC gene Proteins 0.000 claims description 2
- 101100134871 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) aceE gene Proteins 0.000 claims description 2
- SCJNCDSAIRBRIA-DOFZRALJSA-N arachidonyl-2'-chloroethylamide Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(=O)NCCCl SCJNCDSAIRBRIA-DOFZRALJSA-N 0.000 claims description 2
- 101150070136 axeA gene Proteins 0.000 claims description 2
- 239000003053 toxin Substances 0.000 claims description 2
- 231100000765 toxin Toxicity 0.000 claims description 2
- 238000011144 upstream manufacturing Methods 0.000 claims description 2
- 241000193388 Bacillus thuringiensis Species 0.000 claims 1
- 241000239226 Scorpiones Species 0.000 claims 1
- 229940097012 bacillus thuringiensis Drugs 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 17
- 101000868138 Ovis aries Somatotropin Proteins 0.000 description 15
- 108091022908 Serine O-acetyltransferase Proteins 0.000 description 10
- 108020004414 DNA Proteins 0.000 description 8
- 108020004687 Malate Synthase Proteins 0.000 description 8
- 241000699660 Mus musculus Species 0.000 description 8
- 102100038803 Somatotropin Human genes 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 102100034229 Citramalyl-CoA lyase, mitochondrial Human genes 0.000 description 7
- 239000000284 extract Substances 0.000 description 7
- 230000004927 fusion Effects 0.000 description 7
- 210000004185 liver Anatomy 0.000 description 7
- 230000001105 regulatory effect Effects 0.000 description 7
- 238000011830 transgenic mouse model Methods 0.000 description 7
- 101710109085 Cysteine synthase, chloroplastic/chromoplastic Proteins 0.000 description 6
- 108020003285 Isocitrate lyase Proteins 0.000 description 6
- VZXPDPZARILFQX-BYPYZUCNSA-N O-acetyl-L-serine Chemical compound CC(=O)OC[C@H]([NH3+])C([O-])=O VZXPDPZARILFQX-BYPYZUCNSA-N 0.000 description 6
- 101710138316 O-acetylserine sulfhydrylase Proteins 0.000 description 6
- 235000018417 cysteine Nutrition 0.000 description 6
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 6
- 210000003734 kidney Anatomy 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000011701 zinc Substances 0.000 description 5
- DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 4
- 102000003792 Metallothionein Human genes 0.000 description 4
- 108090000157 Metallothionein Proteins 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 101150075677 cysR gene Proteins 0.000 description 4
- 210000000936 intestine Anatomy 0.000 description 4
- 238000002741 site-directed mutagenesis Methods 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 108700003860 Bacterial Genes Proteins 0.000 description 2
- 108010077805 Bacterial Proteins Proteins 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 101000740205 Homo sapiens Sal-like protein 1 Proteins 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 102100037204 Sal-like protein 1 Human genes 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- XVOYSCVBGLVSOL-UHFFFAOYSA-N cysteic acid Chemical group OC(=O)C(N)CS(O)(=O)=O XVOYSCVBGLVSOL-UHFFFAOYSA-N 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 238000001952 enzyme assay Methods 0.000 description 2
- HHLFWLYXYJOTON-UHFFFAOYSA-N glyoxylic acid Chemical compound OC(=O)C=O HHLFWLYXYJOTON-UHFFFAOYSA-N 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 108091027963 non-coding RNA Proteins 0.000 description 2
- 102000042567 non-coding RNA Human genes 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 description 2
- 235000009529 zinc sulphate Nutrition 0.000 description 2
- 239000011686 zinc sulphate Substances 0.000 description 2
- LJGHYPLBDBRCRZ-UHFFFAOYSA-N 3-(3-aminophenyl)sulfonylaniline Chemical compound NC1=CC=CC(S(=O)(=O)C=2C=C(N)C=CC=2)=C1 LJGHYPLBDBRCRZ-UHFFFAOYSA-N 0.000 description 1
- 101100045637 Arabidopsis thaliana TCX7 gene Proteins 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 1
- 241000702224 Enterobacteria phage M13 Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 101710196500 Metallothionein-1A Proteins 0.000 description 1
- 102100029698 Metallothionein-1A Human genes 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 101150027734 cript gene Proteins 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- ODBLHEXUDAPZAU-UHFFFAOYSA-N isocitric acid Chemical compound OC(=O)C(O)C(C(O)=O)CC(O)=O ODBLHEXUDAPZAU-UHFFFAOYSA-N 0.000 description 1
- 229940049920 malate Drugs 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 239000002795 scorpion venom Substances 0.000 description 1
- GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPK566491 | 1991-04-16 | ||
| AUPK5664 | 1991-04-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2108564A1 true CA2108564A1 (fr) | 1992-10-17 |
Family
ID=3775343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002108564A Abandoned CA2108564A1 (fr) | 1991-04-16 | 1992-04-13 | Cassette d'expression genique contenant une sequence non codante du gene de l'hormone de croissance |
Country Status (4)
| Country | Link |
|---|---|
| CA (1) | CA2108564A1 (fr) |
| NZ (1) | NZ242370A (fr) |
| WO (1) | WO1992018635A1 (fr) |
| ZA (1) | ZA922761B (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0742285A1 (fr) * | 1995-05-10 | 1996-11-13 | Rijksuniversiteit Leiden | Méthode pour isoler des segments exoniques de gènes eucaryotes et vecteurs exoniques |
| TW200734460A (en) | 1999-10-04 | 2007-09-16 | Ajinomoto Kk | Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria |
-
1992
- 1992-04-13 CA CA002108564A patent/CA2108564A1/fr not_active Abandoned
- 1992-04-13 WO PCT/AU1992/000164 patent/WO1992018635A1/fr not_active Ceased
- 1992-04-15 ZA ZA922761A patent/ZA922761B/xx unknown
- 1992-04-15 NZ NZ242370A patent/NZ242370A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| WO1992018635A1 (fr) | 1992-10-29 |
| ZA922761B (en) | 1992-12-30 |
| NZ242370A (en) | 1993-02-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Close et al. | Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements | |
| Alexeyev et al. | Membrane topology of the Rickettsia prowazekii ATP/ADP translocase revealed by novel dual pho-lac reporters | |
| US5028530A (en) | AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques | |
| FI84080B (fi) | Foerfarande foer framstaellning av rennin och daeri anvaendbara hybridplasmider. | |
| US6013487A (en) | Chimeric RNA molecules generated by trans-splicing | |
| US5206154A (en) | Method of producing cecropins by microbiological techniques | |
| SK285664B6 (sk) | Purifikovaná a izolovaná transkripčná regulačná DNA škrečacieho EF-1alfa, hostiteľské bunky, vektory, chimerické polynukleotidy a plazmidy | |
| Kruys et al. | Constitutive activity of the tumor necrosis factor promoter is canceled by the 3'untranslated region in nonmacrophage cell lines; a trans-dominant factor overcomes this suppressive effect. | |
| Watson et al. | Construction of improved vectors for protein production in Pseudomonas aeruginosa | |
| FI94774C (fi) | Menetelmiä sekropiinin ja muiden heterologisten polypeptidien tuottamiseksi bakteeri-isännässä araB-promoottoria käyttäen sekä menetelmissä käyttökelpoisia replikoituvia ekspressiovälineitä, geneettisiä muodostelmia ja bakteereja | |
| JP2022527016A (ja) | アミエロイス由来のトランスポザーゼを用いた核酸コンストラクトの真核生物のゲノムへの転移 | |
| JPS5863390A (ja) | Ｄｎａ配列を微生物によつて発現する方法及びその生産物 | |
| CA3108909A1 (fr) | Nouvelles mutations qui ameliorent l'activite de clivage de l'adn de l'acidaminococcus sp. cpf1 | |
| JPS62500074A (ja) | バチルス スリンギエンシス 結晶タンパク質遺伝子毒素セグメント | |
| US5614398A (en) | Gene transfer system for insects | |
| CA2108564A1 (fr) | Cassette d'expression genique contenant une sequence non codante du gene de l'hormone de croissance | |
| JPS61291600A (ja) | 工学処理されたハイブリツド遺伝子で製造された活性化バシルススリンギエンシスδ−エンドトキシン | |
| JPS58141778A (ja) | 金属チオネインの発現 | |
| Kalosaka et al. | Evaluation of the activities of the medfly and Drosophila hsp70 promoters in vivo in germ‐line transformed medflies | |
| Lee et al. | Gene Expression of Cotesiaplutellae Bracovlrus EP1-like Protein (CpBV-ELP1) in Parasitized Diamondback Moth, Plutellae xylostella | |
| AU655815B2 (en) | Gene expression cassette containing non-coding sequence of growth hormone gene | |
| JP5013375B2 (ja) | メッセンジャーｒｎａ干渉酵素が促進する生細胞における単一タンパク質産生 | |
| RU2125091C1 (ru) | Рекомбинантная плазмидная днкрtv241, способ конструирования рекомбинантной плазмидной днкрtv241, способ конструирования плазмиды, содержащей ген cry iii a в составе транспозона tn917cat, штамм бактерий bacillus thuringiensis subsp. thuringiensis, активный против колорадского жука | |
| ES2204980T3 (es) | Nuevo promotor y metodo de expresion de genes que lo utiliza. | |
| ES2257853T3 (es) | Construcciones para la expresion controlada de genes recombinantes en celulas procariotas. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |