CA2103021A1 - Adn recombinant codant pour un peptide signal, polypeptide a interaction selective et sequence d'ancrage membranaire - Google Patents

Adn recombinant codant pour un peptide signal, polypeptide a interaction selective et sequence d'ancrage membranaire

Info

Publication number
CA2103021A1
CA2103021A1 CA2103021A CA2103021A CA2103021A1 CA 2103021 A1 CA2103021 A1 CA 2103021A1 CA 2103021 A CA2103021 A CA 2103021A CA 2103021 A CA2103021 A CA 2103021A CA 2103021 A1 CA2103021 A1 CA 2103021A1
Authority
CA
Canada
Prior art keywords
gram positive
recombinant dna
signal peptide
sequence
dna fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2103021A
Other languages
English (en)
Other versions
CA2103021C (fr
Inventor
Stefan Stahl
Per-Ake Nygren
Marianne Hansson
Mathias Uhlen
Thien N. Nguyen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Affibody AB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2103021A1 publication Critical patent/CA2103021A1/fr
Application granted granted Critical
Publication of CA2103021C publication Critical patent/CA2103021C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C07K14/445—Plasmodium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
    • C12N2760/18522—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/882—Staphylococcus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/882—Staphylococcus
    • Y10S435/883—Staphylococcus aureus

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Une séquence d'ADN recombiné comprend un premier fragment d'ADN codant pour une première séquence d'acides aminés servant de peptide signal pouvant agir dans un hôte à Gram positif, relié fonctionnellement à un deuxième fragment d'ADN codant pour une deuxième séquence d'acides aminés qui ne sont pas normalement présents sur la surface des bactéries à Gram négatif, et capable d'une interaction sélective, ledit deuxième fragment d'ADN étant relié fonctionnellement à un troisième fragment d'ADN codant pour une troisième séquence d'acides aminés pouvant agir dans un hôte à Gram positif à titre de séquence de franchissement de la paroi cellulaire et d'ancrage de membrane; un vecteur d'expression ou plasmide contenant ladite séquence d'ADN recombiné; une cellule bactérienne à Gram positif renfermant un tel vecteur ou plasmide; et un procédé pour l'isolation sélective ou l'identification de cellules bactériennes à Gram positif.
CA002103021A 1991-05-13 1992-05-11 Adn recombinant codant pour un peptide signal, polypeptide a interaction selective et sequence d'ancrage membranaire Expired - Lifetime CA2103021C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9101433-2 1991-05-13
SE9101433A SE9101433D0 (sv) 1991-05-13 1991-05-13 Recombinant dna sequence and its use
PCT/SE1992/000304 WO1992020805A1 (fr) 1991-05-13 1992-05-11 Adn recombine codant pour un peptide signal, polypeptide d'interaction et sequence d'ancrage de membrane

Publications (2)

Publication Number Publication Date
CA2103021A1 true CA2103021A1 (fr) 1992-12-01
CA2103021C CA2103021C (fr) 2000-07-04

Family

ID=20382707

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002103021A Expired - Lifetime CA2103021C (fr) 1991-05-13 1992-05-11 Adn recombinant codant pour un peptide signal, polypeptide a interaction selective et sequence d'ancrage membranaire

Country Status (10)

Country Link
US (1) US5958736A (fr)
EP (1) EP0584167B1 (fr)
JP (1) JP3423712B2 (fr)
AT (1) ATE270335T1 (fr)
AU (1) AU664841B2 (fr)
CA (1) CA2103021C (fr)
DE (1) DE69233375T2 (fr)
OA (1) OA09866A (fr)
SE (1) SE9101433D0 (fr)
WO (1) WO1992020805A1 (fr)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
US6737521B1 (en) * 1990-05-11 2004-05-18 The Rockefeller University Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria
CA2131995A1 (fr) * 1992-03-13 1993-09-16 Vincent A. Fischetti Procede de livraison et d'expression d'une proteine de surface hybride a la surface de bacteries gram positives
EP0682710B1 (fr) * 1993-02-10 2003-10-29 Unilever Plc Procede d'isolation utilisant des proteines immobilisees a capacites de fixation specifique
US6740734B1 (en) * 1994-01-14 2004-05-25 Biovitrum Ab Bacterial receptor structures
FR2718452B1 (fr) * 1994-04-06 1996-06-28 Pf Medicament Elément d'immunogène, agent immunogène, composition pharmaceutique et procédé de préparation.
FR2726577B1 (fr) * 1994-11-07 1997-01-31 Pf Medicament Procede d'obtention d'un peptide derive du virus respiratoire syncitial, polypeptide et bacterie l'exprimant, et leurs applications a titre de medicament
FR2726471B1 (fr) * 1994-11-07 1997-01-31 Pf Medicament Procede pour ameliorer l'immunogenicite d'un compose immunogene ou d'un haptene et application a la preparation de vaccins
CN1160469C (zh) 1994-12-09 2004-08-04 帝国大学改革有限公司 基因的鉴定
FR2766192B1 (fr) * 1997-07-17 2001-07-13 Pf Medicament Epitopes du vrs et anticorps les comportant, utiles dans le diagnostic et la therapie
FR2766091A1 (fr) * 1997-07-18 1999-01-22 Transgene Sa Composition antitumorale a base de polypeptide immunogene de localisation cellulaire modifiee
WO2000014240A2 (fr) 1998-09-04 2000-03-16 Creatogen Aktiengesellschaft Mutants attenues de spi2 de salmonelles utilises comme porteurs d'antigenes
GB9910812D0 (en) 1999-05-10 1999-07-07 Microscience Ltd Vaccine composition
EP1377306A1 (fr) * 2001-03-09 2004-01-07 Dyax Corp. Groupes de liaison d'albumine serique
EP2075256A2 (fr) 2002-01-14 2009-07-01 William Herman Ligands ciblés
EP1532173A4 (fr) * 2002-06-14 2006-03-08 Dyax Corp Analyse de proteines
US20040071705A1 (en) * 2002-06-21 2004-04-15 Dyax Corporation Serum protein-associated target-specific ligands and identification method therefor
WO2008127457A2 (fr) * 2006-12-06 2008-10-23 Repligen Corporation Acides nucléiques codant pour la protéine recombinée a
US8017337B2 (en) * 2007-04-19 2011-09-13 Molecular Detection, Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
WO2009158240A1 (fr) 2008-06-16 2009-12-30 Emergent Product Development Uk Limited Vaccins vectorisés de salmonella contre chlamydia et procédés d’utilisation
WO2016197064A1 (fr) 2015-06-04 2016-12-08 Epstein Alan L Immunothérapie des cellules car ciblées par lym-1 et lym-2
JP7196104B2 (ja) 2017-06-05 2022-12-26 リサーチ インスティチュート アット ネイションワイド チルドレンズ ホスピタル 増強された改変ウイルスカプシドタンパク質
AU2020311897A1 (en) 2019-07-08 2022-02-03 Research Institute At Nationwide Children's Hospital Antibody compositions for disrupting biofilms
AU2022358523A1 (en) 2021-09-30 2024-03-14 The Board Of Regents Of The Universityof Texas System Slc13a5 gene therapy vectors and uses thereof
WO2023096996A2 (fr) 2021-11-24 2023-06-01 Research Institute At Nationwide Children's Hospital Hsv chimère exprimant hil21 pour renforcer l'activité immunitaire antitumorale
WO2023102406A1 (fr) 2021-12-01 2023-06-08 The Board Of Regents Of The Univesity Of Texas System Conception de génome de vecteur pour exprimer un transgène cln7 optimisé
WO2023102518A1 (fr) 2021-12-03 2023-06-08 The Board Of Regents Of The University Of Texas System Vecteurs de thérapie génique gna01 et leurs utilisations
WO2023147476A1 (fr) 2022-01-28 2023-08-03 The Board Of Regents Of The University Of Texas System Cassette transgénique conçue pour exprimer le gène fmr1 humain à codons optimisés

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0109861A3 (fr) * 1982-11-23 1986-05-14 Bio-Response Inc. Procédé pour isoler des cellules
US5200327A (en) * 1985-11-06 1993-04-06 Cangene Corporation Expression system for the secretion of bioactive human granulocyte macrophage colony stimulating factor (GM-CSF) and other heterologous proteins from streptomyces
US4859609A (en) * 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
DE3852304T3 (de) * 1987-03-02 1999-07-01 Enzon Lab Inc Organismus als Träger für "Single Chain Antibody Domain (SCAD)".
DE3744595A1 (de) * 1987-12-31 1989-07-13 Andreas Dr Plueckthun Verfahren zur gentechnischen herstellung von antikoerpern
WO1989007140A1 (fr) * 1988-02-05 1989-08-10 Commonwealth Scientific And Industrial Research Or Systeme d'expression par genes (en particulier pour la proteine de rotavirus vp7) employant un peptide de signal etranger et eventuellement une sequence d'ancrage transmembrane

Also Published As

Publication number Publication date
WO1992020805A1 (fr) 1992-11-26
SE9101433D0 (sv) 1991-05-13
CA2103021C (fr) 2000-07-04
EP0584167B1 (fr) 2004-06-30
US5958736A (en) 1999-09-28
AU664841B2 (en) 1995-12-07
EP0584167A1 (fr) 1994-03-02
ATE270335T1 (de) 2004-07-15
JP3423712B2 (ja) 2003-07-07
AU1789992A (en) 1992-12-30
JPH07502640A (ja) 1995-03-23
OA09866A (en) 1994-08-15
DE69233375D1 (de) 2004-08-05
DE69233375T2 (de) 2005-07-14

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