CA2064092A1 - Systeme efficace de clonage genetique directionnel - Google Patents

Systeme efficace de clonage genetique directionnel

Info

Publication number
CA2064092A1
CA2064092A1 CA2064092A CA2064092A CA2064092A1 CA 2064092 A1 CA2064092 A1 CA 2064092A1 CA 2064092 A CA2064092 A CA 2064092A CA 2064092 A CA2064092 A CA 2064092A CA 2064092 A1 CA2064092 A1 CA 2064092A1
Authority
CA
Canada
Prior art keywords
cloning
cdna
vector
inserts
vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2064092A
Other languages
English (en)
Other versions
CA2064092C (fr
Inventor
Toru Miki
Stuart A. Aaronson
Timothy Fleming
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Commerce
Original Assignee
Toru Miki
Stuart A. Aaronson
Timothy Fleming
The United States Of America, Represented By The Secretary, U.S. Departm Ent Of Commerce
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toru Miki, Stuart A. Aaronson, Timothy Fleming, The United States Of America, Represented By The Secretary, U.S. Departm Ent Of Commerce filed Critical Toru Miki
Publication of CA2064092A1 publication Critical patent/CA2064092A1/fr
Application granted granted Critical
Publication of CA2064092C publication Critical patent/CA2064092C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
CA002064092A 1989-07-28 1990-07-30 Systeme efficace de clonage genetique directionnel Expired - Lifetime CA2064092C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38605389A 1989-07-28 1989-07-28
US386,053 1989-07-28

Publications (2)

Publication Number Publication Date
CA2064092A1 true CA2064092A1 (fr) 1991-01-29
CA2064092C CA2064092C (fr) 1996-05-21

Family

ID=23523965

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002064092A Expired - Lifetime CA2064092C (fr) 1989-07-28 1990-07-30 Systeme efficace de clonage genetique directionnel

Country Status (6)

Country Link
US (1) US5595895A (fr)
EP (2) EP0773294A3 (fr)
JP (2) JPH04503309A (fr)
AU (2) AU649228B2 (fr)
CA (1) CA2064092C (fr)
WO (1) WO1991002077A1 (fr)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100267023A1 (en) * 1992-09-24 2010-10-21 Keygene N.V. Selective restriction fragment amplification: fingerprinting
US5866755A (en) * 1993-06-14 1999-02-02 Basf Aktiengellschaft Animals transgenic for a tetracycline-regulated transcriptional inhibitor
US5859310A (en) 1993-06-14 1999-01-12 Basf Aktiengesellschaft Mice transgenic for a tetracycline-controlled transcriptional activator
US5650298A (en) 1993-06-14 1997-07-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5888981A (en) 1993-06-14 1999-03-30 Basf Aktiengesellschaft Methods for regulating gene expression
US5912411A (en) * 1993-06-14 1999-06-15 University Of Heidelberg Mice transgenic for a tetracycline-inducible transcriptional activator
US6004941A (en) * 1993-06-14 1999-12-21 Basf Aktiengesellschaft Methods for regulating gene expression
US6150141A (en) * 1993-09-10 2000-11-21 Trustees Of Boston University Intron-mediated recombinant techniques and reagents
US5597719A (en) * 1994-07-14 1997-01-28 Onyx Pharmaceuticals, Inc. Interaction of RAF-1 and 14-3-3 proteins
US6825011B1 (en) 1998-12-17 2004-11-30 Yuri Rumantichikov Methods for insertion of nucleic acids into circular vectors
US5948646A (en) 1997-12-11 1999-09-07 Fordham University Methods for preparation of vaccines against cancer comprising heat shock protein-peptide complexes
ATE359084T1 (de) 1998-02-20 2007-05-15 Univ Miami Modifizierter hitzeschockprotein/peptidantigen komplex
US6653077B1 (en) 1998-09-04 2003-11-25 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
JP2002537762A (ja) 1999-01-05 2002-11-12 トラスティーズ オブ ボストン ユニバーシティ 改良型核酸クローニング
US20040005673A1 (en) 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
EP1176200A3 (fr) 2000-06-20 2005-01-12 Switch Biotech Aktiengesellschaft Utilisation des polypeptides ou leurs acides nucléiques pour le diagnose ou traitement des maladies de la peau ou de la cicatrisation de blessures et leurs utilisations pour l'identification des substances pharmacologiquement actives
EP1205548A1 (fr) * 2000-11-09 2002-05-15 Kabushiki Kaisha Toyota Chuo Kenkyusho Méthode et dispositif pour la ligation d'ADN double brin sur support solide
US20040038283A1 (en) * 2001-04-30 2004-02-26 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
US6902914B2 (en) * 2001-09-28 2005-06-07 Sigma-Aldrich, Co. Recombinant DNA processes using a dNTP mixture containing modified nucleotides
US7205130B2 (en) * 2002-05-08 2007-04-17 Rigel Pharmaceuticals, Inc. Bi-directionally cloned random cDNA expression vector libraries, compositions and methods of use
DE60332948D1 (de) * 2002-07-19 2010-07-22 Althea Technologies Inc Strategien zur genexpressionsanalyse
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
CA2407825A1 (fr) * 2002-10-11 2004-04-11 Andrew J. Simmonds Marquage par la methode trap : nouvelle methode d'identification et de purification de complexes arn-proteine
ATE461292T1 (de) * 2003-09-10 2010-04-15 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
CA2541177A1 (fr) * 2003-10-03 2005-09-22 Promega Corporation Vecteurs pour le clonage directionnel
CN100465278C (zh) * 2004-06-10 2009-03-04 清华大学 一种构建t载体的方法
EP1997898A1 (fr) * 2007-06-01 2008-12-03 Rijksuniversiteit Groningen Outils et procédé de clonage de séquences d'acides nucléiques
WO2009079212A2 (fr) 2007-12-03 2009-06-25 Carnegie Mellon University Biocapteurs fluorogéniques de peptides liés
AU2009223838B2 (en) 2008-03-03 2012-07-26 The University Of Miami Allogeneic cancer cell-based immunotherapy
CA2718884C (fr) 2008-03-20 2016-11-22 University Of Miami Vaccination par proteine de choc thermique gp96 et procedes d'utilisation
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
WO2013191950A2 (fr) 2012-06-22 2013-12-27 Monsanto Technology Llc Conception d'un vecteur modulaire unique
WO2014142955A1 (fr) * 2013-03-15 2014-09-18 Carnegie Mellon University Biocapteurs fluorogéniques à peptides liés
RU2714157C2 (ru) 2015-02-06 2020-02-12 Хит Байолоджикс, Инк. Вектор, коэкспрессирующий молекулы для вакцинации и костимулирующие молекулы
US11618899B2 (en) 2016-02-09 2023-04-04 Brookhaven Science Associates, Llc Cloning and expression vectors and systems
WO2018071405A1 (fr) 2016-10-11 2018-04-19 University Of Miami Vecteurs et cellules de vaccin pour immunité contre le virus zika
CA3058938A1 (fr) 2017-04-04 2018-10-11 Heat Biologics, Inc. Vaccination intratumorale

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001230A (en) * 1988-02-18 1991-03-19 Schering Corporation T cell activation markers

Also Published As

Publication number Publication date
EP0484440A1 (fr) 1992-05-13
AU6187990A (en) 1991-03-11
JPH04503309A (ja) 1992-06-18
AU6894894A (en) 1994-11-17
JPH07255491A (ja) 1995-10-09
AU671228B2 (en) 1996-08-15
EP0773294A3 (fr) 1997-09-17
US5595895A (en) 1997-01-21
EP0773294A2 (fr) 1997-05-14
WO1991002077A1 (fr) 1991-02-21
CA2064092C (fr) 1996-05-21
EP0484440A4 (en) 1993-01-07
AU649228B2 (en) 1994-05-19

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