CA2058999A1 - Vaccin contraceptif base sur un gene clone de la zone pellucide - Google Patents
Vaccin contraceptif base sur un gene clone de la zone pellucideInfo
- Publication number
- CA2058999A1 CA2058999A1 CA002058999A CA2058999A CA2058999A1 CA 2058999 A1 CA2058999 A1 CA 2058999A1 CA 002058999 A CA002058999 A CA 002058999A CA 2058999 A CA2058999 A CA 2058999A CA 2058999 A1 CA2058999 A1 CA 2058999A1
- Authority
- CA
- Canada
- Prior art keywords
- protein
- epitope
- amino acid
- contraceptive
- mouse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 98
- 210000004340 zona pellucida Anatomy 0.000 title claims abstract description 75
- 229940124462 contraceptive vaccine Drugs 0.000 title claims abstract description 32
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 124
- 101000976442 Homo sapiens Zona pellucida sperm-binding protein 3 Proteins 0.000 claims abstract description 106
- 102100023634 Zona pellucida sperm-binding protein 3 Human genes 0.000 claims abstract description 95
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 83
- 230000002254 contraceptive effect Effects 0.000 claims abstract description 72
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 71
- 239000003433 contraceptive agent Substances 0.000 claims abstract description 67
- 229960005486 vaccine Drugs 0.000 claims abstract description 59
- 229920001184 polypeptide Polymers 0.000 claims abstract description 54
- 241000894007 species Species 0.000 claims abstract description 37
- 101000976425 Mus musculus Zona pellucida sperm-binding protein 3 Proteins 0.000 claims abstract description 30
- 210000000287 oocyte Anatomy 0.000 claims abstract description 26
- 230000027455 binding Effects 0.000 claims abstract description 25
- 102100021422 Zona pellucida sperm-binding protein 2 Human genes 0.000 claims abstract description 19
- 101000818870 Homo sapiens Zona pellucida sperm-binding protein 2 Proteins 0.000 claims abstract description 18
- 230000004720 fertilization Effects 0.000 claims abstract description 17
- 102000055956 human ZP3 Human genes 0.000 claims abstract description 12
- 101000818877 Homo sapiens Zona pellucida sperm-binding protein 1 Proteins 0.000 claims abstract description 7
- 102100021401 Zona pellucida sperm-binding protein 1 Human genes 0.000 claims abstract description 7
- 101100268281 Mus musculus Zp2 gene Proteins 0.000 claims abstract description 6
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 62
- 108020004414 DNA Proteins 0.000 claims description 31
- 241000282414 Homo sapiens Species 0.000 claims description 24
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 13
- 210000004027 cell Anatomy 0.000 claims description 12
- 101100545381 Mus musculus Zp3 gene Proteins 0.000 claims description 11
- 230000005875 antibody response Effects 0.000 claims description 9
- 241000124008 Mammalia Species 0.000 claims description 7
- 239000002671 adjuvant Substances 0.000 claims description 5
- 230000002708 enhancing effect Effects 0.000 claims description 5
- 108020004511 Recombinant DNA Proteins 0.000 claims description 4
- 241000282326 Felis catus Species 0.000 claims description 3
- 239000013598 vector Substances 0.000 claims description 3
- 102000053602 DNA Human genes 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 206010060935 Alloimmunisation Diseases 0.000 abstract description 9
- 235000018102 proteins Nutrition 0.000 description 73
- 239000000427 antigen Substances 0.000 description 50
- 102000036639 antigens Human genes 0.000 description 50
- 108091007433 antigens Proteins 0.000 description 50
- 235000001014 amino acid Nutrition 0.000 description 49
- 229940024606 amino acid Drugs 0.000 description 48
- 150000001413 amino acids Chemical class 0.000 description 47
- 241000699666 Mus <mouse, genus> Species 0.000 description 35
- 239000002299 complementary DNA Substances 0.000 description 31
- 210000003719 b-lymphocyte Anatomy 0.000 description 26
- 241001465754 Metazoa Species 0.000 description 24
- 241000699670 Mus sp. Species 0.000 description 19
- 230000003053 immunization Effects 0.000 description 19
- 238000002649 immunization Methods 0.000 description 19
- 108020004999 messenger RNA Proteins 0.000 description 19
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 16
- 239000002773 nucleotide Substances 0.000 description 15
- 125000003729 nucleotide group Chemical group 0.000 description 14
- 210000001672 ovary Anatomy 0.000 description 14
- 239000012634 fragment Substances 0.000 description 12
- 230000002611 ovarian Effects 0.000 description 12
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 11
- 108091028043 Nucleic acid sequence Proteins 0.000 description 10
- 241000283973 Oryctolagus cuniculus Species 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 230000028993 immune response Effects 0.000 description 10
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 9
- 241001529936 Murinae Species 0.000 description 8
- 239000002253 acid Substances 0.000 description 8
- 238000013459 approach Methods 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 108020004635 Complementary DNA Proteins 0.000 description 7
- 241000287828 Gallus gallus Species 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 7
- 238000011161 development Methods 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 230000035558 fertility Effects 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 6
- 108700026244 Open Reading Frames Proteins 0.000 description 6
- 101150088854 Zp3 gene Proteins 0.000 description 6
- 230000002209 hydrophobic effect Effects 0.000 description 6
- 210000000987 immune system Anatomy 0.000 description 6
- 230000002163 immunogen Effects 0.000 description 6
- 230000005847 immunogenicity Effects 0.000 description 6
- 229940023041 peptide vaccine Drugs 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 102000003886 Glycoproteins Human genes 0.000 description 5
- 108090000288 Glycoproteins Proteins 0.000 description 5
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 108091034057 RNA (poly(A)) Proteins 0.000 description 5
- 239000000562 conjugate Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 241000282472 Canis lupus familiaris Species 0.000 description 4
- 101000818886 Mus musculus Zona pellucida sperm-binding protein 2 Proteins 0.000 description 4
- 238000000636 Northern blotting Methods 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000000890 antigenic effect Effects 0.000 description 4
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- 238000010353 genetic engineering Methods 0.000 description 4
- 230000001900 immune effect Effects 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- 230000013011 mating Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 3
- 108700024394 Exon Proteins 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 241000700159 Rattus Species 0.000 description 3
- 230000000961 alloantigen Effects 0.000 description 3
- 230000003698 anagen phase Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- -1 designated ZPl Proteins 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 210000004201 immune sera Anatomy 0.000 description 3
- 229940042743 immune sera Drugs 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 229920001220 nitrocellulos Polymers 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 229920000136 polysorbate Polymers 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- RMVRSNDYEFQCLF-UHFFFAOYSA-N thiophenol Chemical compound SC1=CC=CC=C1 RMVRSNDYEFQCLF-UHFFFAOYSA-N 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 241000283707 Capra Species 0.000 description 2
- 108091033380 Coding strand Proteins 0.000 description 2
- 241000699800 Cricetinae Species 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 108020005350 Initiator Codon Proteins 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 2
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 230000004988 N-glycosylation Effects 0.000 description 2
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- 241000700618 Vaccinia virus Species 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- RDOXTESZEPMUJZ-UHFFFAOYSA-N anisole Chemical compound COC1=CC=CC=C1 RDOXTESZEPMUJZ-UHFFFAOYSA-N 0.000 description 2
- 210000002469 basement membrane Anatomy 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 235000018417 cysteine Nutrition 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000016087 ovulation Effects 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 230000006337 proteolytic cleavage Effects 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- LVSPDZAGCBEQAV-UHFFFAOYSA-N 4-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=CC=C(Cl)C2=C1 LVSPDZAGCBEQAV-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 108010032595 Antibody Binding Sites Proteins 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 101100545378 Homo sapiens ZP3 gene Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 101710203526 Integrase Proteins 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 125000000769 L-threonyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])[C@](O[H])(C([H])([H])[H])[H] 0.000 description 1
- 241001599018 Melanogaster Species 0.000 description 1
- 241001024304 Mino Species 0.000 description 1
- 108090000143 Mouse Proteins Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 241000277275 Oncorhynchus mykiss Species 0.000 description 1
- 108091036407 Polyadenylation Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 101150016788 SERPINA10 gene Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 241000258128 Strongylocentrotus purpuratus Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 241000269368 Xenopus laevis Species 0.000 description 1
- 102000008937 Zona Pellucida Glycoproteins Human genes 0.000 description 1
- 108010074006 Zona Pellucida Glycoproteins Proteins 0.000 description 1
- 239000004015 abortifacient agent Substances 0.000 description 1
- 231100000641 abortifacient agent Toxicity 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000002788 anti-peptide Effects 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000007698 birth defect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 230000005889 cellular cytotoxicity Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229940124558 contraceptive agent Drugs 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007402 cytotoxic response Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 108010043837 egg surface sperm receptor Proteins 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- 108010032819 exoribonuclease II Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229910000040 hydrogen fluoride Inorganic materials 0.000 description 1
- 230000008073 immune recognition Effects 0.000 description 1
- 230000006058 immune tolerance Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 239000000568 immunological adjuvant Substances 0.000 description 1
- 230000006054 immunological memory Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 208000021267 infertility disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- UZKWTJUDCOPSNM-UHFFFAOYSA-N methoxybenzene Substances CCCCOC=C UZKWTJUDCOPSNM-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 150000002482 oligosaccharides Polymers 0.000 description 1
- 230000008775 paternal effect Effects 0.000 description 1
- 239000000863 peptide conjugate Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 210000004896 polypeptide structure Anatomy 0.000 description 1
- 230000000270 postfertilization Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000031877 prophase Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000013077 scoring method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 108010087967 type I signal peptidase Proteins 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Reproductive Health (AREA)
- Endocrinology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36437989A | 1989-06-12 | 1989-06-12 | |
| US364,379 | 1989-06-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2058999A1 true CA2058999A1 (fr) | 1990-12-13 |
Family
ID=23434271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002058999A Abandoned CA2058999A1 (fr) | 1989-06-12 | 1990-06-06 | Vaccin contraceptif base sur un gene clone de la zone pellucide |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0477226A4 (fr) |
| JP (2) | JPH05500654A (fr) |
| AU (1) | AU636895B2 (fr) |
| CA (1) | CA2058999A1 (fr) |
| WO (1) | WO1990015624A1 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5641487A (en) * | 1989-06-12 | 1997-06-24 | The Government Of The United States Of America As Represented By The Secretary Department Of Health And Human Services | Contraceptive vaccine based on alloimmunization with zona pellucida polypeptides |
| JPH06500690A (ja) * | 1990-08-27 | 1994-01-27 | アクゾ・エヌ・ヴエー | ヒト卵透明帯タンパク質ｚｐ３ |
| AU9094191A (en) * | 1990-11-21 | 1992-06-25 | Washington University | Recombinant avirulent salmonella antifertility vaccines |
| JPH05317050A (ja) * | 1992-01-10 | 1993-12-03 | Shinzou Isojima | Ｐｚｐ−４遺伝子及び避妊ワクチン |
| WO1994010304A1 (fr) * | 1992-11-02 | 1994-05-11 | Akzo Nobel N.V. | Proteine zp3 de la zone pellucide du tamarin |
| US6001599A (en) * | 1992-11-09 | 1999-12-14 | Zonagen, Inc. | DNAs encoding mammalian ZPBs |
| WO1994011019A1 (fr) * | 1992-11-09 | 1994-05-26 | Zonagen, Inc. | Materiaux et procedes utiles pour l'immunocontraception |
| JP3537471B2 (ja) * | 1993-09-24 | 2004-06-14 | 麒麟麦酒株式会社 | 新規蛋白質ｓｐ３８および避妊ワクチン |
| CN1158623A (zh) * | 1994-08-22 | 1997-09-03 | 阿克佐诺贝尔公司 | 新的免疫避孕肽 |
| US7019114B2 (en) | 1998-02-19 | 2006-03-28 | Eastern Virginia Medical School | Recombinant, biologically active human zona pellucida protein 3 (HZP3) to test male fertility |
| US7037663B2 (en) | 1998-02-19 | 2006-05-02 | Eastern Virginia Medical School | Human zona pellucida protein 3 and uses thereof |
| CA2456821A1 (fr) | 2001-08-02 | 2003-02-13 | Trinity Biomedical Technology Corporation | Proteines de zone pellucide humaine et methodes d'utilisation associees pour le diagnostic de la sterilite masculine |
| CN101696239B (zh) * | 2009-09-29 | 2011-07-27 | 上海市计划生育科学研究所 | 人卵透明带蛋白多表位嵌合肽抗原及其制备方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3992520A (en) * | 1974-11-27 | 1976-11-16 | Merck & Co., Inc. | Anti-fertility vaccine comprising solubilized zona pellucida and anti-serum |
| JPS61140553A (ja) * | 1984-12-07 | 1986-06-27 | メルク フロスト カナダ， インコ−ポレ−テツド | ロイコトリエンとタンパク質との結合体 |
| US4795634A (en) * | 1986-03-14 | 1989-01-03 | Aphton Corporation | Method for contraception by immunization against the zona pellucida |
| US4996297A (en) * | 1987-10-07 | 1991-02-26 | Zonagen, Inc. | Recombinantly expressed rabbit zona pellucida polypeptides |
-
1990
- 1990-06-06 JP JP2508831A patent/JPH05500654A/ja active Pending
- 1990-06-06 EP EP19900909085 patent/EP0477226A4/en not_active Withdrawn
- 1990-06-06 WO PCT/US1990/003075 patent/WO1990015624A1/fr not_active Application Discontinuation
- 1990-06-06 CA CA002058999A patent/CA2058999A1/fr not_active Abandoned
- 1990-06-06 AU AU58267/90A patent/AU636895B2/en not_active Ceased
-
1998
- 1998-03-19 JP JP10070452A patent/JPH10262671A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO1990015624A1 (fr) | 1990-12-27 |
| EP0477226A4 (en) | 1992-09-30 |
| AU5826790A (en) | 1991-01-08 |
| AU636895B2 (en) | 1993-05-13 |
| EP0477226A1 (fr) | 1992-04-01 |
| JPH10262671A (ja) | 1998-10-06 |
| JPH05500654A (ja) | 1993-02-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU684765B2 (en) | Contraceptive vaccine based on alloimmunization with zona pellucida polypeptides | |
| AU636895B2 (en) | Contraceptive vaccine based on cloned zona pellucida gene | |
| AU2006201502A1 (en) | Sperm specific proteins | |
| CA2040441C (fr) | Proteines de fusion | |
| CA2090486A1 (fr) | Proteine zp3 de la membrane pellucide humaine | |
| AU622044B2 (en) | Fusion proteins | |
| JP2787926B2 (ja) | 融合タンパク質 | |
| Hasegawa et al. | Evaluation of the contraceptive potential of recombinant proteins and synthetic peptides of zona pellucida (ZP) | |
| AU6656398A (en) | Method for the production of vaccines against cell surface proteins | |
| WO1997039020A2 (fr) | Sequences antigeniques d'une proteine seminale et methodes immunocontraceptives |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |