CA2025929A1 - Methode d'essais de complementation enzymatique de proteines de liaison - Google Patents

Methode d'essais de complementation enzymatique de proteines de liaison

Info

Publication number
CA2025929A1
CA2025929A1 CA 2025929 CA2025929A CA2025929A1 CA 2025929 A1 CA2025929 A1 CA 2025929A1 CA 2025929 CA2025929 CA 2025929 CA 2025929 A CA2025929 A CA 2025929A CA 2025929 A1 CA2025929 A1 CA 2025929A1
Authority
CA
Canada
Prior art keywords
enzyme
complementation assays
protein binding
binding enzyme
enzyme complementation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2025929
Other languages
English (en)
Other versions
CA2025929C (fr
Inventor
Daniel R. Henderson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microgenics Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2025929A1 publication Critical patent/CA2025929A1/fr
Application granted granted Critical
Publication of CA2025929C publication Critical patent/CA2025929C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CA 2025929 1989-09-22 1990-09-21 Methode d'essais de complementation enzymatique de proteines de liaison Expired - Lifetime CA2025929C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41099689A 1989-09-22 1989-09-22
US410,996 1989-09-22

Publications (2)

Publication Number Publication Date
CA2025929A1 true CA2025929A1 (fr) 1991-03-23
CA2025929C CA2025929C (fr) 1999-11-23

Family

ID=23627124

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2025929 Expired - Lifetime CA2025929C (fr) 1989-09-22 1990-09-21 Methode d'essais de complementation enzymatique de proteines de liaison

Country Status (4)

Country Link
EP (1) EP0419081A3 (fr)
JP (2) JPH03179263A (fr)
AU (1) AU649961B2 (fr)
CA (1) CA2025929C (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223393A (en) * 1990-06-12 1993-06-29 Microgenics Corporation Detection of analytes having binding sites for at least two binding moieties
CA2075858C (fr) * 1991-08-15 1998-05-19 Scott J. Eisenbeis Methode de detection de sequences nucleotidiques complementaires
CA2118913C (fr) * 1991-09-13 2008-08-19 Donald Leonard Nicholas Cardy Essai d'extension d'acides nucleiques
EP0817789B1 (fr) * 1996-01-26 2002-01-02 Roche Diagnostics Corporation Agents de reticulation bis-maleimido
US5976857A (en) * 1996-01-26 1999-11-02 Boehringer Mannheim Corporation Cross-linked polypeptide fragments of β-galactosidase
US5763196A (en) * 1996-01-26 1998-06-09 Boehringer Mannheim Corporation Assays using cross-linked polypeptide fragments of β-galactosidase
US6828099B2 (en) * 1998-02-02 2004-12-07 Odyssey Thera Inc. Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 β-Lactamase
WO2005100975A1 (fr) * 2004-04-13 2005-10-27 Shimadzu Corporation Procédé de mesure d'une substance par spectrométrie de masse et kit correspondant
US7354996B2 (en) 2004-09-07 2008-04-08 Shimadzu Corporation Method and kit for quantitative analysis of protein
CN105524898B (zh) * 2016-01-22 2019-01-11 美康生物科技股份有限公司 一种克隆酶供体片段及其制备方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4378428A (en) * 1981-03-30 1983-03-29 Baker Instruments Corporation Method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
US4708929A (en) * 1984-10-29 1987-11-24 Microgenics Corporation Methods for protein binding enzyme complementation assays
AU592324B2 (en) * 1984-10-29 1990-01-11 Roche Diagnostics Corporation Mehods for protein binding enzyme complementation assays
DE3724625A1 (de) * 1987-07-24 1989-02-02 Boehringer Mannheim Gmbh Enzymatisch inaktive, immunologisch aktive ss-galactosidase-muteine
DE68914305T2 (de) * 1988-02-02 1994-10-06 Microgenics Corp Rezeptorpreincubations-Enzymtest.
WO1990005749A1 (fr) * 1988-11-18 1990-05-31 The Regents Of The University Of California Polypeptides conjugues, leurs procedes de preparation et d'utilisation
EP0472593B1 (fr) * 1989-05-05 1996-08-14 Microgenics Corporation Methode d'analyse de complementarisation d'enzymes fixatrices de proteine

Also Published As

Publication number Publication date
AU649961B2 (en) 1994-06-09
EP0419081A3 (en) 1992-07-22
JP2000023684A (ja) 2000-01-25
JPH03179263A (ja) 1991-08-05
EP0419081A2 (fr) 1991-03-27
CA2025929C (fr) 1999-11-23
AU6267790A (en) 1991-04-11

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