ES8606500A1 - Un metodo para determinar la presencia de ige especifica en sueros humanos - Google Patents
Un metodo para determinar la presencia de ige especifica en sueros humanosInfo
- Publication number
- ES8606500A1 ES8606500A1 ES545787A ES545787A ES8606500A1 ES 8606500 A1 ES8606500 A1 ES 8606500A1 ES 545787 A ES545787 A ES 545787A ES 545787 A ES545787 A ES 545787A ES 8606500 A1 ES8606500 A1 ES 8606500A1
- Authority
- ES
- Spain
- Prior art keywords
- determining
- solid support
- human sera
- specific ige
- incubation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 239000007787 solid Substances 0.000 abstract 3
- 239000000284 extract Substances 0.000 abstract 2
- 238000011534 incubation Methods 0.000 abstract 2
- 238000005406 washing Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
METODO DE DETECCION DE LA PRESENCIA DE IGE ESPECIFICA EN SUEROS HUMANOS. COMPRENDE LA FIJACION DE EXTRACTOS PROTEICOS COMPLEJOS, PREVIAMENTE PURIFICADOS, A UN SOPORTE SOLIDO, DE FORMA COVALENTE O NO COVALENTE; SEGUIDA DE LA INCUBACION DE LA MUESTRA CON LOS EXTRACTOS PROTEICOS FIJADOS; LAVADO DE LAS PROTEINAS NO ESPECIFICAS; INCUBACION DE LAS MISMAS CON UN ANTICUERPO MONOCLONAL ANTI-IGE, MARCADO CON 125I O CON UNA ENZIMA, TAL COMO B-GALACTOSIDASA; LAVADO DEL SOPORTE SOLIDO CON LOS PRODUCOS FIJADOS; Y, FINALMENTE DETERMINACION DE LA RADIACTIVIDAD INCORPORADA AL MISMO O DE LA ACTIVIDAD ENZIMATICA. LOS VALORES OBTENIDOS SE COMPARAN CON UN SUERO PATRON Y SE OBTIENE UNA APRECIACION SEMICUANTITATIVA. TIENE APLICACIONES EN TECNICAS INMUNOLOGICAS Y EN EL CAMPO DEL DIAGNOSTICO.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES545787A ES8606500A1 (es) | 1985-07-31 | 1985-07-31 | Un metodo para determinar la presencia de ige especifica en sueros humanos |
| IT8621272A IT1213466B (it) | 1985-07-31 | 1986-07-25 | Metodo per determinare la presenza di ige specifica in sieri umani. |
| PT83105A PT83105B (pt) | 1985-07-31 | 1986-07-30 | Processo para a determinacao da presenca de ige especifica em soros humanos |
| CH3048/86A CH668650A5 (fr) | 1985-07-31 | 1986-07-30 | Procede pour determiner la concentration d'ige specifique dans les serums humains. |
| FR868611067A FR2585837B1 (fr) | 1985-07-31 | 1986-07-30 | Procede pour determiner la presence de ige specifique dans des serums humains |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES545787A ES8606500A1 (es) | 1985-07-31 | 1985-07-31 | Un metodo para determinar la presencia de ige especifica en sueros humanos |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8606500A1 true ES8606500A1 (es) | 1986-04-01 |
| ES545787A0 ES545787A0 (es) | 1986-04-01 |
Family
ID=8489662
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES545787A Expired ES8606500A1 (es) | 1985-07-31 | 1985-07-31 | Un metodo para determinar la presencia de ige especifica en sueros humanos |
Country Status (5)
| Country | Link |
|---|---|
| CH (1) | CH668650A5 (es) |
| ES (1) | ES8606500A1 (es) |
| FR (1) | FR2585837B1 (es) |
| IT (1) | IT1213466B (es) |
| PT (1) | PT83105B (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES8606501A1 (es) * | 1985-07-31 | 1986-04-01 | Abello Alergia & Inmunologia | Un metodo para determinar la presencia de igg especifica en sueros humanos |
| ES8606499A1 (es) * | 1985-07-31 | 1986-04-01 | Abello Alergia & Inmunologia | Un procedimiento para la valoracion de la concentracion de inmunoglobulina e en sueros humanos |
| JP2988635B2 (ja) * | 1990-09-18 | 1999-12-13 | 塩野義製薬株式会社 | ヒトＩｇＥに対するモノクローナル抗体 |
| FR2698694A1 (fr) * | 1992-12-01 | 1994-06-03 | Bordeaux I Universite | Moyens pour la détection in vitro d'IgE dirigées contre un nouvel allergène d'insectes lépidoptères: la thaumétopoéine. |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4444879A (en) * | 1981-01-29 | 1984-04-24 | Science Research Center, Inc. | Immunoassay with article having support film and immunological counterpart of analyte |
| US4539292A (en) * | 1982-12-29 | 1985-09-03 | Reid Michael J | Immunoassay technique for specific immunoglobulin E |
| EP0119613B1 (en) * | 1983-03-17 | 1992-07-29 | BioWhittaker, Inc. | Fluorometric assay of total ige level and reagents therefor |
| FR2556840B1 (fr) * | 1983-12-16 | 1987-12-24 | Immunotech Sa | Procede de dosage in vitro des immunoglobulines specifiques d'un ou plusieurs allergenes et reactifs et moyens pour la mise en oeuvre de ce procede |
-
1985
- 1985-07-31 ES ES545787A patent/ES8606500A1/es not_active Expired
-
1986
- 1986-07-25 IT IT8621272A patent/IT1213466B/it active
- 1986-07-30 CH CH3048/86A patent/CH668650A5/fr not_active IP Right Cessation
- 1986-07-30 PT PT83105A patent/PT83105B/pt not_active IP Right Cessation
- 1986-07-30 FR FR868611067A patent/FR2585837B1/fr not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| PT83105B (pt) | 1988-07-01 |
| IT1213466B (it) | 1989-12-20 |
| FR2585837B1 (fr) | 1991-07-26 |
| IT8621272A0 (it) | 1986-07-25 |
| PT83105A (en) | 1986-08-01 |
| FR2585837A1 (fr) | 1987-02-06 |
| ES545787A0 (es) | 1986-04-01 |
| CH668650A5 (fr) | 1989-01-13 |
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