CA2024607A1 - Methode de production d'adn recombinant et cellules hote - Google Patents

Methode de production d'adn recombinant et cellules hote

Info

Publication number
CA2024607A1
CA2024607A1 CA2024607A CA2024607A CA2024607A1 CA 2024607 A1 CA2024607 A1 CA 2024607A1 CA 2024607 A CA2024607 A CA 2024607A CA 2024607 A CA2024607 A CA 2024607A CA 2024607 A1 CA2024607 A1 CA 2024607A1
Authority
CA
Canada
Prior art keywords
host cells
recombinant dna
dna method
amount
transcription unit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2024607A
Other languages
English (en)
Other versions
CA2024607C (fr
Inventor
Mark Ian Cockett
Christopher Robert Bebbington
Geoffrey Thomas Yarranton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Group AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2024607A1 publication Critical patent/CA2024607A1/fr
Application granted granted Critical
Publication of CA2024607C publication Critical patent/CA2024607C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne un procédé et une cellules hôte permettant d'augmenter la production d'une protéine voulue par une lignée de cellules. Ledit procédé et la lignée de cellule utilisent une première unité de transcription contenant un gène pour une protéine de transactivation, qui commande la transactivation d'une seconde unité de transcription, de sorte que l'on peut augmenter la quantité d'une expression de protéine voulue sans affecter de manière contraire la croissance des cellules. Les protéines de transactivation sont de préférence dérivées du gène E1A.
CA002024607A 1989-01-10 1990-01-10 Methode de production d'adn recombinant et cellules hote Expired - Lifetime CA2024607C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB8900483.2 1989-01-10
GB898900483A GB8900483D0 (en) 1989-01-10 1989-01-10 Recombinant dna method
PCT/GB1990/000032 WO1990008191A1 (fr) 1989-01-10 1990-01-10 Procede de recombinaison d'adn et cellules hotes

Publications (2)

Publication Number Publication Date
CA2024607A1 true CA2024607A1 (fr) 1990-07-11
CA2024607C CA2024607C (fr) 1999-11-30

Family

ID=10649831

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002024607A Expired - Lifetime CA2024607C (fr) 1989-01-10 1990-01-10 Methode de production d'adn recombinant et cellules hote

Country Status (12)

Country Link
US (2) US5866359A (fr)
EP (1) EP0378382B1 (fr)
JP (1) JP3070950B2 (fr)
AT (1) ATE153382T1 (fr)
AU (2) AU4825390A (fr)
CA (1) CA2024607C (fr)
DE (1) DE69030740T2 (fr)
DK (1) DK0378382T3 (fr)
ES (1) ES2103266T3 (fr)
GB (1) GB8900483D0 (fr)
GR (1) GR3024002T3 (fr)
WO (1) WO1990008191A1 (fr)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method
EP0455424A3 (en) * 1990-05-02 1992-04-29 Merck & Co. Inc. Mammalian inducible promoter cascade system
CA2047633A1 (fr) * 1990-09-12 1992-03-13 Ben Bowen Inactivation de la traduction genetique che les plantes au moyen d'activateurs modifies de la traduction
DK0566554T3 (da) 1992-03-13 2000-08-07 Monsanto Co Fremstilling af rekombinante proteiner ved anvendelse af herpesviruspromotorer og VP16-transaktivatorer
US5585237A (en) * 1993-10-25 1996-12-17 Creative Biomolecules, Inc. Methods and compositions for high protein production from recombinant DNA
US5631158A (en) * 1993-10-25 1997-05-20 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
DE69638058D1 (de) 1995-06-15 2009-11-26 Crucell Holland Bv Verpackungssysteme für humane rekombinante Adenoviren zur Gentherapie
US7604960B2 (en) * 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US7297680B2 (en) * 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US20050170463A1 (en) * 1999-04-15 2005-08-04 Abraham Bout Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins
US8236561B2 (en) * 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US20050164386A1 (en) * 1999-04-15 2005-07-28 Uytdehaag Alphonsus G. Overexpression of enzymes involved in post-translational protein modifications in human cells
IL145849A0 (en) * 1999-04-15 2002-07-25 Crucell Holland Bv Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein
US7527961B2 (en) * 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
US7192759B1 (en) * 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
US7521220B2 (en) * 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
US20030119104A1 (en) * 2001-05-30 2003-06-26 Edward Perkins Chromosome-based platforms
ATE384785T1 (de) * 2001-12-07 2008-02-15 Crucell Holland Bv Herstellung von viren, virusisolaten, und impfstoffen
DK1623023T3 (da) 2003-05-09 2009-03-02 Crucell Holland Bv Kulturer af E1-immortaliserede celler og fremgangsmåder til dyrkning deraf til forögelse af produktudbytter derfra
ATE469920T1 (de) * 2003-05-23 2010-06-15 Crucell Holland Bv Herstellung von rekombinanten igm in den per.c6 zellen
EP1702071B1 (fr) 2003-12-31 2012-02-22 Kalobios Inc. Systeme de transactivation de cellules mammaliennes
ES2540753T3 (es) 2012-09-24 2015-07-13 Lonza Biologics Plc. Vectores de expresión que comprenden secuencias quiméricas de promotor y amplificador de citomegalovirus
EP2829608A1 (fr) 2013-07-23 2015-01-28 Universität Bielefeld Procédé de production de protéine recombinante dans des cellules de mammifères

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
EP0245949B1 (fr) * 1986-04-09 1997-10-29 Eli Lilly And Company Méthode d'utilisation des vecteurs d'expression eucaryotiques comportant les séquences stimulantes du virus BK
US4775630A (en) * 1986-08-15 1988-10-04 Vanderbilt University Transcriptional control element adapted for regulation of gene expression in animal cells
EP0282330A3 (fr) * 1987-03-13 1988-11-09 Schering Corporation Procédé pour stimuler l'expression de gènes
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method

Also Published As

Publication number Publication date
JP3070950B2 (ja) 2000-07-31
US6653101B1 (en) 2003-11-25
WO1990008191A1 (fr) 1990-07-26
EP0378382B1 (fr) 1997-05-21
US5866359A (en) 1999-02-02
DE69030740T2 (de) 1997-11-27
DK0378382T3 (da) 1997-06-16
JPH03503959A (ja) 1991-09-05
ATE153382T1 (de) 1997-06-15
EP0378382A1 (fr) 1990-07-18
AU662416B2 (en) 1995-08-31
AU3994193A (en) 1993-08-12
AU4825390A (en) 1990-08-13
GR3024002T3 (en) 1997-10-31
DE69030740D1 (de) 1997-06-26
GB8900483D0 (en) 1989-03-08
CA2024607C (fr) 1999-11-30
ES2103266T3 (es) 1997-09-16

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