CA2024607A1 - Methode de production d'adn recombinant et cellules hote - Google Patents
Methode de production d'adn recombinant et cellules hoteInfo
- Publication number
- CA2024607A1 CA2024607A1 CA2024607A CA2024607A CA2024607A1 CA 2024607 A1 CA2024607 A1 CA 2024607A1 CA 2024607 A CA2024607 A CA 2024607A CA 2024607 A CA2024607 A CA 2024607A CA 2024607 A1 CA2024607 A1 CA 2024607A1
- Authority
- CA
- Canada
- Prior art keywords
- host cells
- recombinant dna
- dna method
- amount
- transcription unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 3
- 108020004511 Recombinant DNA Proteins 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 102000040945 Transcription factor Human genes 0.000 abstract 2
- 108091023040 Transcription factor Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 230000035897 transcription Effects 0.000 abstract 2
- 238000013518 transcription Methods 0.000 abstract 2
- 230000002411 adverse Effects 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
La présente invention concerne un procédé et une cellules hôte permettant d'augmenter la production d'une protéine voulue par une lignée de cellules. Ledit procédé et la lignée de cellule utilisent une première unité de transcription contenant un gène pour une protéine de transactivation, qui commande la transactivation d'une seconde unité de transcription, de sorte que l'on peut augmenter la quantité d'une expression de protéine voulue sans affecter de manière contraire la croissance des cellules. Les protéines de transactivation sont de préférence dérivées du gène E1A.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8900483.2 | 1989-01-10 | ||
GB898900483A GB8900483D0 (en) | 1989-01-10 | 1989-01-10 | Recombinant dna method |
PCT/GB1990/000032 WO1990008191A1 (fr) | 1989-01-10 | 1990-01-10 | Procede de recombinaison d'adn et cellules hotes |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2024607A1 true CA2024607A1 (fr) | 1990-07-11 |
CA2024607C CA2024607C (fr) | 1999-11-30 |
Family
ID=10649831
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002024607A Expired - Lifetime CA2024607C (fr) | 1989-01-10 | 1990-01-10 | Methode de production d'adn recombinant et cellules hote |
Country Status (12)
Country | Link |
---|---|
US (2) | US5866359A (fr) |
EP (1) | EP0378382B1 (fr) |
JP (1) | JP3070950B2 (fr) |
AT (1) | ATE153382T1 (fr) |
AU (2) | AU4825390A (fr) |
CA (1) | CA2024607C (fr) |
DE (1) | DE69030740T2 (fr) |
DK (1) | DK0378382T3 (fr) |
ES (1) | ES2103266T3 (fr) |
GB (1) | GB8900483D0 (fr) |
GR (1) | GR3024002T3 (fr) |
WO (1) | WO1990008191A1 (fr) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8900483D0 (en) | 1989-01-10 | 1989-03-08 | Celltech Ltd | Recombinant dna method |
EP0455424A3 (en) * | 1990-05-02 | 1992-04-29 | Merck & Co. Inc. | Mammalian inducible promoter cascade system |
CA2047633A1 (fr) * | 1990-09-12 | 1992-03-13 | Ben Bowen | Inactivation de la traduction genetique che les plantes au moyen d'activateurs modifies de la traduction |
PT566554E (pt) * | 1992-03-13 | 2000-09-29 | Monsanto Co | Producao de proteinas recombinantes usando promotores do virus de herpes e transactivadores vp16 |
US5733782A (en) * | 1993-10-25 | 1998-03-31 | Creative Biomolecules, Inc. | Methods and compositions for high protein production from non-native DNA |
US5585237A (en) * | 1993-10-25 | 1996-12-17 | Creative Biomolecules, Inc. | Methods and compositions for high protein production from recombinant DNA |
IL122614A0 (en) | 1995-06-15 | 1998-08-16 | Introgene Bv | Packaging systems for human recombinant adenovirus to be used in gene therapy |
US7297680B2 (en) * | 1999-04-15 | 2007-11-20 | Crucell Holland B.V. | Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content |
US20050170463A1 (en) * | 1999-04-15 | 2005-08-04 | Abraham Bout | Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins |
US7604960B2 (en) * | 1999-04-15 | 2009-10-20 | Crucell Holland B.V. | Transient protein expression methods |
US8236561B2 (en) * | 1999-04-15 | 2012-08-07 | Crucell Holland B.V. | Efficient production of IgA in recombinant mammalian cells |
DK2163640T3 (da) * | 1999-04-15 | 2012-03-05 | Crucell Holland Bv | Rekombinant proteinproduktion i en human celle indeholdende mindst ét E1-protein af adenovirus |
US20050164386A1 (en) * | 1999-04-15 | 2005-07-28 | Uytdehaag Alphonsus G. | Overexpression of enzymes involved in post-translational protein modifications in human cells |
US6855544B1 (en) * | 1999-04-15 | 2005-02-15 | Crucell Holland B.V. | Recombinant protein production in a human cell |
US7527961B2 (en) * | 1999-11-26 | 2009-05-05 | Crucell Holland B.V. | Production of vaccines |
US7521220B2 (en) * | 1999-11-26 | 2009-04-21 | Crucell Holland B.V. | Production of vaccines |
US7192759B1 (en) * | 1999-11-26 | 2007-03-20 | Crucell Holland B.V. | Production of vaccines |
WO2002097059A2 (fr) * | 2001-05-30 | 2002-12-05 | Chromos Molecular Systems, Inc. | Plate-formes basees sur des chromosomes |
ATE384785T1 (de) * | 2001-12-07 | 2008-02-15 | Crucell Holland Bv | Herstellung von viren, virusisolaten, und impfstoffen |
DK1623023T3 (da) * | 2003-05-09 | 2009-03-02 | Crucell Holland Bv | Kulturer af E1-immortaliserede celler og fremgangsmåder til dyrkning deraf til forögelse af produktudbytter derfra |
EP1626992B1 (fr) * | 2003-05-23 | 2010-06-02 | Crucell Holland B.V. | Production d'une igm recombinante dans des cellules per.c6 |
US8354251B2 (en) | 2003-12-31 | 2013-01-15 | Kalobios Pharmaceuticals, Inc. | Transactivation system for mammalian cells |
PL2711426T3 (pl) | 2012-09-24 | 2015-09-30 | Lonza Biologics Plc | Wektory ekspresyjne zawierające chimeryczne sekwencje promotora i wzmacniacza wirusa cytomegalii |
EP2829608A1 (fr) | 2013-07-23 | 2015-01-28 | Universität Bielefeld | Procédé de production de protéine recombinante dans des cellules de mammifères |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4740461A (en) * | 1983-12-27 | 1988-04-26 | Genetics Institute, Inc. | Vectors and methods for transformation of eucaryotic cells |
EP0245949B1 (fr) * | 1986-04-09 | 1997-10-29 | Eli Lilly And Company | Méthode d'utilisation des vecteurs d'expression eucaryotiques comportant les séquences stimulantes du virus BK |
US4775630A (en) * | 1986-08-15 | 1988-10-04 | Vanderbilt University | Transcriptional control element adapted for regulation of gene expression in animal cells |
JPH02502605A (ja) * | 1987-03-13 | 1990-08-23 | シェリング・コーポレーション | 遺伝子発現の刺激方法 |
US5024939A (en) * | 1987-07-09 | 1991-06-18 | Genentech, Inc. | Transient expression system for producing recombinant protein |
GB8900483D0 (en) | 1989-01-10 | 1989-03-08 | Celltech Ltd | Recombinant dna method |
-
1989
- 1989-01-10 GB GB898900483A patent/GB8900483D0/en active Pending
-
1990
- 1990-01-10 DK DK90300270.7T patent/DK0378382T3/da active
- 1990-01-10 DE DE69030740T patent/DE69030740T2/de not_active Expired - Lifetime
- 1990-01-10 EP EP90300270A patent/EP0378382B1/fr not_active Expired - Lifetime
- 1990-01-10 JP JP2501787A patent/JP3070950B2/ja not_active Expired - Lifetime
- 1990-01-10 CA CA002024607A patent/CA2024607C/fr not_active Expired - Lifetime
- 1990-01-10 WO PCT/GB1990/000032 patent/WO1990008191A1/fr active Application Filing
- 1990-01-10 AU AU48253/90A patent/AU4825390A/en not_active Abandoned
- 1990-01-10 AT AT90300270T patent/ATE153382T1/de not_active IP Right Cessation
- 1990-01-10 ES ES90300270T patent/ES2103266T3/es not_active Expired - Lifetime
-
1993
- 1993-05-31 AU AU39941/93A patent/AU662416B2/en not_active Expired
-
1995
- 1995-05-16 US US08/442,646 patent/US5866359A/en not_active Expired - Lifetime
-
1997
- 1997-07-08 GR GR970401654T patent/GR3024002T3/el unknown
-
2000
- 2000-11-03 US US09/704,800 patent/US6653101B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU662416B2 (en) | 1995-08-31 |
DK0378382T3 (da) | 1997-06-16 |
CA2024607C (fr) | 1999-11-30 |
EP0378382B1 (fr) | 1997-05-21 |
JPH03503959A (ja) | 1991-09-05 |
ATE153382T1 (de) | 1997-06-15 |
WO1990008191A1 (fr) | 1990-07-26 |
US6653101B1 (en) | 2003-11-25 |
GR3024002T3 (en) | 1997-10-31 |
AU3994193A (en) | 1993-08-12 |
DE69030740T2 (de) | 1997-11-27 |
EP0378382A1 (fr) | 1990-07-18 |
ES2103266T3 (es) | 1997-09-16 |
US5866359A (en) | 1999-02-02 |
JP3070950B2 (ja) | 2000-07-31 |
GB8900483D0 (en) | 1989-03-08 |
AU4825390A (en) | 1990-08-13 |
DE69030740D1 (de) | 1997-06-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |