CA2020572C - Process for the preservation of plant embryos - Google Patents
Process for the preservation of plant embryos Download PDFInfo
- Publication number
- CA2020572C CA2020572C CA002020572A CA2020572A CA2020572C CA 2020572 C CA2020572 C CA 2020572C CA 002020572 A CA002020572 A CA 002020572A CA 2020572 A CA2020572 A CA 2020572A CA 2020572 C CA2020572 C CA 2020572C
- Authority
- CA
- Canada
- Prior art keywords
- embryo
- oil
- coated
- embryos
- process according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000002257 embryonic structure Anatomy 0.000 title claims abstract description 128
- 238000000034 method Methods 0.000 title claims description 30
- 238000004321 preservation Methods 0.000 title description 5
- 206010021143 Hypoxia Diseases 0.000 claims abstract description 40
- 230000007954 hypoxia Effects 0.000 claims abstract description 40
- 230000035601 cold sensitivity Effects 0.000 claims abstract description 15
- 239000011248 coating agent Substances 0.000 claims abstract description 11
- 238000000576 coating method Methods 0.000 claims abstract description 11
- 239000003921 oil Substances 0.000 claims description 76
- 210000001161 mammalian embryo Anatomy 0.000 claims description 56
- 239000001963 growth medium Substances 0.000 claims description 31
- 241000196324 Embryophyta Species 0.000 claims description 22
- 230000000392 somatic effect Effects 0.000 claims description 16
- 238000009630 liquid culture Methods 0.000 claims description 12
- 240000007154 Coffea arabica Species 0.000 claims description 8
- 235000007460 Coffea arabica Nutrition 0.000 claims description 8
- 244000000626 Daucus carota Species 0.000 claims description 7
- 235000002767 Daucus carota Nutrition 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 229920000642 polymer Polymers 0.000 claims description 6
- 239000005662 Paraffin oil Substances 0.000 claims description 5
- 229940057995 liquid paraffin Drugs 0.000 claims description 4
- 239000011347 resin Substances 0.000 claims description 4
- 229920005989 resin Polymers 0.000 claims description 4
- 239000000122 growth hormone Substances 0.000 claims description 3
- 239000002480 mineral oil Substances 0.000 claims description 2
- 235000010446 mineral oil Nutrition 0.000 claims description 2
- 235000013311 vegetables Nutrition 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims 1
- 238000003860 storage Methods 0.000 abstract description 25
- 230000035899 viability Effects 0.000 description 21
- 239000002609 medium Substances 0.000 description 17
- 238000011161 development Methods 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 241000894007 species Species 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 239000006285 cell suspension Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 206010020649 Hyperkeratosis Diseases 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 2
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 230000030118 somatic embryogenesis Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8909639 | 1989-07-18 | ||
| FR8909639A FR2649860A1 (fr) | 1989-07-18 | 1989-07-18 | Procede de conservation d'embryons vegetaux |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2020572A1 CA2020572A1 (en) | 1991-01-19 |
| CA2020572C true CA2020572C (en) | 2000-03-28 |
Family
ID=9383871
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002020572A Expired - Fee Related CA2020572C (en) | 1989-07-18 | 1990-07-06 | Process for the preservation of plant embryos |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5138793A (esLanguage) |
| EP (1) | EP0408922B1 (esLanguage) |
| JP (1) | JPH03101601A (esLanguage) |
| AP (1) | AP158A (esLanguage) |
| AT (1) | ATE113072T1 (esLanguage) |
| AU (1) | AU629369B2 (esLanguage) |
| BR (1) | BR9003442A (esLanguage) |
| CA (1) | CA2020572C (esLanguage) |
| DE (1) | DE69013423T2 (esLanguage) |
| DK (1) | DK0408922T3 (esLanguage) |
| ES (1) | ES2064534T3 (esLanguage) |
| FI (1) | FI96375C (esLanguage) |
| FR (1) | FR2649860A1 (esLanguage) |
| IE (1) | IE65060B1 (esLanguage) |
| MX (1) | MX171281B (esLanguage) |
| MY (1) | MY105818A (esLanguage) |
| NO (1) | NO179352C (esLanguage) |
| NZ (1) | NZ234524A (esLanguage) |
| OA (1) | OA09218A (esLanguage) |
| PT (1) | PT94720B (esLanguage) |
| ZA (1) | ZA905204B (esLanguage) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6340594B1 (en) | 1991-12-19 | 2002-01-22 | Cellfor, Inc. | Production of desiccation-tolerant gymnosperm embryos |
| US5985667A (en) * | 1991-12-19 | 1999-11-16 | University Of Saskatchewan | Maturation, desiccation and encapsulation of gymnosperm somatic embryos |
| NZ272210A (en) * | 1995-05-25 | 1997-10-24 | Carter Holt Harvey Ltd | Treatment of somatic embryos to provide viable embryos after storage periods |
| EP0816488A1 (fr) * | 1996-06-25 | 1998-01-07 | Societe Des Produits Nestle S.A. | Procédé de conditionnement de tissus de végétaux cultivés in-vitro |
| JP4476442B2 (ja) * | 2000-06-20 | 2010-06-09 | アグリテクノ矢崎株式会社 | トルコギキョウのロゼット化による発育不良防止方法 |
| WO2002101018A2 (en) * | 2001-06-11 | 2002-12-19 | Fred Hutchinson Cancer Research Center | Methods for inducing reversible stasis |
| CA2450211C (en) | 2001-06-28 | 2011-03-22 | Morishita Jintan Co., Ltd. | Capsules containing vital cells or tissues |
| US20120131849A1 (en) * | 2009-06-25 | 2012-05-31 | Masanori Asada | Method for storage of seeds |
| EP2725887A4 (en) * | 2011-06-29 | 2015-01-07 | Weyerhaeuser Nr Co | ARTIFICIAL SEED WITH AN EMBRYO IN IT |
| US20150191771A1 (en) * | 2014-01-09 | 2015-07-09 | Syngenta Participations Ag | Embryo sampling method |
| US9078427B1 (en) * | 2014-08-29 | 2015-07-14 | Pioneer Hi Bred International Inc | Method of storing plant embryos |
| US9706723B2 (en) * | 2014-08-29 | 2017-07-18 | Pioneer Hi-Bred International, Inc. | Methods for creating doubled haploid maize embryos using oil matrices |
| US11111548B2 (en) * | 2014-08-29 | 2021-09-07 | Pioneer Hi-Bred International, Inc. | Systems and methods for genotyping seed components |
| WO2016032629A1 (en) | 2014-08-29 | 2016-03-03 | Pioneer Hi-Bred International, Inc. | Methods and devices involving oil matrices |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2091534B (en) * | 1980-12-09 | 1984-06-27 | Boc Lit | Method of preservation |
| US4397681A (en) * | 1981-06-08 | 1983-08-09 | Fats And Proteins Research Foundation, Inc. | Foliar antitranspirant |
| US4583320A (en) * | 1982-10-12 | 1986-04-22 | Plant Genetics, Inc. | Delivery system for meristematic tissue |
| GB8323094D0 (en) * | 1983-08-26 | 1983-09-28 | Franks F | Preservation of cells |
| JPS6140708A (ja) * | 1984-07-31 | 1986-02-27 | ライオン株式会社 | 人工種子 |
| US4615141A (en) * | 1984-08-14 | 1986-10-07 | Purdue Research Foundation | Process for encapsulating asexual plant embryos |
| JPS62138107A (ja) * | 1985-12-10 | 1987-06-20 | フロイント産業株式会社 | 組成物およびその製造方法 |
| JPS62257320A (ja) * | 1986-04-28 | 1987-11-09 | 株式会社 日本グリンナ− | 穂発芽の予防、抑制法 |
| WO1989005575A1 (en) * | 1987-12-18 | 1989-06-29 | University Of Florida | Quiescent plant somatic embryos and method for their production |
| DE3882528T2 (de) * | 1988-09-19 | 1993-11-11 | Nestle Sa | Verfahren zur Produktion von PflanzensubstanzEN. |
-
1989
- 1989-07-18 FR FR8909639A patent/FR2649860A1/fr active Granted
-
1990
- 1990-06-22 DE DE69013423T patent/DE69013423T2/de not_active Expired - Fee Related
- 1990-06-22 ES ES90111821T patent/ES2064534T3/es not_active Expired - Lifetime
- 1990-06-22 AT AT90111821T patent/ATE113072T1/de active
- 1990-06-22 EP EP90111821A patent/EP0408922B1/fr not_active Expired - Lifetime
- 1990-06-22 DK DK90111821.6T patent/DK0408922T3/da active
- 1990-06-25 US US07/543,475 patent/US5138793A/en not_active Expired - Lifetime
- 1990-06-27 FI FI903229A patent/FI96375C/fi not_active IP Right Cessation
- 1990-06-27 IE IE232590A patent/IE65060B1/en not_active IP Right Cessation
- 1990-07-02 AU AU58674/90A patent/AU629369B2/en not_active Ceased
- 1990-07-03 ZA ZA905204A patent/ZA905204B/xx unknown
- 1990-07-06 CA CA002020572A patent/CA2020572C/en not_active Expired - Fee Related
- 1990-07-09 MX MX021506A patent/MX171281B/es unknown
- 1990-07-13 NO NO903138A patent/NO179352C/no not_active IP Right Cessation
- 1990-07-16 AP APAP/P/1990/000193A patent/AP158A/en active
- 1990-07-16 NZ NZ234524A patent/NZ234524A/xx unknown
- 1990-07-16 MY MYPI90001188A patent/MY105818A/en unknown
- 1990-07-17 JP JP2189262A patent/JPH03101601A/ja active Pending
- 1990-07-17 PT PT94720A patent/PT94720B/pt not_active IP Right Cessation
- 1990-07-17 BR BR909003442A patent/BR9003442A/pt not_active IP Right Cessation
- 1990-07-18 OA OA59819A patent/OA09218A/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MX171281B (es) | 1993-10-15 |
| ZA905204B (en) | 1991-04-24 |
| JPH03101601A (ja) | 1991-04-26 |
| AP9000193A0 (en) | 1990-07-31 |
| DE69013423D1 (de) | 1994-11-24 |
| NO179352C (no) | 1996-09-25 |
| ATE113072T1 (de) | 1994-11-15 |
| AU5867490A (en) | 1991-01-24 |
| EP0408922B1 (fr) | 1994-10-19 |
| IE65060B1 (en) | 1995-10-04 |
| CA2020572A1 (en) | 1991-01-19 |
| FI96375B (fi) | 1996-03-15 |
| PT94720A (pt) | 1991-03-20 |
| DK0408922T3 (da) | 1995-02-27 |
| EP0408922A1 (fr) | 1991-01-23 |
| PT94720B (pt) | 1997-07-31 |
| AU629369B2 (en) | 1992-10-01 |
| FR2649860A1 (fr) | 1991-01-25 |
| NZ234524A (en) | 1991-10-25 |
| US5138793A (en) | 1992-08-18 |
| NO903138D0 (no) | 1990-07-13 |
| AP158A (en) | 1991-11-15 |
| NO903138L (no) | 1991-01-21 |
| MY105818A (en) | 1995-01-30 |
| ES2064534T3 (es) | 1995-02-01 |
| DE69013423T2 (de) | 1995-02-23 |
| BR9003442A (pt) | 1991-08-27 |
| FR2649860B1 (esLanguage) | 1994-08-19 |
| FI96375C (fi) | 1996-06-25 |
| FI903229A0 (fi) | 1990-06-27 |
| OA09218A (en) | 1992-06-30 |
| IE902325A1 (en) | 1991-06-19 |
| NO179352B (no) | 1996-06-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |