CA2012025A1 - Adn pour l'expression et la secretion - Google Patents

Adn pour l'expression et la secretion

Info

Publication number
CA2012025A1
CA2012025A1 CA2012025A CA2012025A CA2012025A1 CA 2012025 A1 CA2012025 A1 CA 2012025A1 CA 2012025 A CA2012025 A CA 2012025A CA 2012025 A CA2012025 A CA 2012025A CA 2012025 A1 CA2012025 A1 CA 2012025A1
Authority
CA
Canada
Prior art keywords
dna
nucleotide
aforementioned
concerning
derivative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2012025A
Other languages
English (en)
Other versions
CA2012025C (fr
Inventor
Yukiko Tsukuda
Yasushi Kojima
Yukio Kita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New Oji Paper Co Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP6185989A external-priority patent/JPH02242685A/ja
Priority claimed from JP2047160A external-priority patent/JP3036777B2/ja
Application filed by Individual filed Critical Individual
Publication of CA2012025A1 publication Critical patent/CA2012025A1/fr
Application granted granted Critical
Publication of CA2012025C publication Critical patent/CA2012025C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13007—Phenol 2-monooxygenase (1.14.13.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002012025A 1989-03-14 1990-03-13 Adn pour l'expression et la secretion Expired - Fee Related CA2012025C (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP61859/1989 1989-03-14
JP6185989A JPH02242685A (ja) 1989-03-14 1989-03-14 フェノールオキシダーゼ遺伝子組換えｄｎａ、該組換えｄｎａにより形質転換された酵母、その培養物及びフェノールオキシダーゼの製造方法
JP6540089 1989-03-17
JP65400/1989 1989-03-17
JP2047160A JP3036777B2 (ja) 1989-03-17 1990-03-01 発現及び分泌に関するｄｎａ
JP47160/1990 1990-03-01

Publications (2)

Publication Number Publication Date
CA2012025A1 true CA2012025A1 (fr) 1990-09-14
CA2012025C CA2012025C (fr) 1999-09-07

Family

ID=27292891

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002012025A Expired - Fee Related CA2012025C (fr) 1989-03-14 1990-03-13 Adn pour l'expression et la secretion

Country Status (4)

Country Link
US (1) US6075138A (fr)
EP (1) EP0388166B1 (fr)
CA (1) CA2012025C (fr)
DE (1) DE69025081T2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2091236C (fr) * 1992-03-09 2000-06-20 Akira Tsukamoto Gene de l'ornithine carbamoyl-transferase et utilisation de l'adn
EP1015600A1 (fr) * 1997-08-22 2000-07-05 Dsm N.V. Production de statine par fermentation
US6387688B1 (en) * 1999-02-15 2002-05-14 Oji Paper Co., Ltd. DNA fragments having basidiomycete-derived promoter activity and expression of foreign genes under control of the promoter activity
DE112008001989A5 (de) * 2007-07-31 2010-06-10 Basf Plant Science Gmbh Desaturasen und Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4695455A (en) * 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
JPS61285989A (ja) * 1985-06-13 1986-12-16 Oji Paper Co Ltd フエノ−ルオキシダ−ゼおよびその製造方法
JPH0671424B2 (ja) * 1986-03-18 1994-09-14 新王子製紙株式会社 リグニン分解酵素およびその製造方法
AU606081B2 (en) * 1986-09-12 1991-01-31 Sandoz Ag Recombinant ligninase
US5055294A (en) * 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
USH875H (en) * 1988-03-18 1991-01-01 E. I. Du Pont De Nemours And Company, Inc. Toxin-encoding nucleic acid fragments derived from a Bacillus thuringiensis subsp. israelensis gene
JPH0227985A (ja) * 1988-07-15 1990-01-30 Oji Paper Co Ltd フェノールオキシダーゼ遺伝子（１）
CA2091236C (fr) * 1992-03-09 2000-06-20 Akira Tsukamoto Gene de l'ornithine carbamoyl-transferase et utilisation de l'adn

Also Published As

Publication number Publication date
EP0388166A1 (fr) 1990-09-19
CA2012025C (fr) 1999-09-07
US6075138A (en) 2000-06-13
DE69025081T2 (de) 1996-05-30
DE69025081D1 (de) 1996-03-14
EP0388166B1 (fr) 1996-01-31

Similar Documents

Publication Publication Date Title
Felmlee et al. Escherichia coli hemolysin is released extracellularly without cleavage of a signal peptide
Saxena et al. Identification of a new gene in an operon for cellulose biosynthesis in Acetobacter xylinum
KR880014110A (ko) 인체 프로아포지방단백질 a-i의 발현
DE3485284D1 (de) Klonieren von superoxid-dismutase und expression in mikroorganismen.
CA2032153A1 (fr) Application de nouveaux fragments d'adn en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
DK0562003T3 (da) Forbedret sukkerdannelse fra cellulose ved kloning og amplificering af beta-glucosidase-genet fra Tricoderma reesei
WO2002074964A3 (fr) Polypeptides derives des arn polymerases, et leurs utilisations
ATE310090T1 (de) Gereinigte coprinus laccasen und nukleinsäuren welche dafür kodieren
DE3783191D1 (de) Verfahren und mittel zur herstellung eines proteins mit der gleichen igg-spezifizitaet wie protein g.
DK1826271T3 (da) Termofilt svampeekspressionssystem
WO1995006735A3 (fr) Sequences nucleotidiques servant a coder de nouvelles tyrosine-phosphatases proteiques
Bilge et al. mRNA processing independent of RNase III and RNase E in the expression of the F1845 fimbrial adhesin of Escherichia coli.
CA2072375A1 (fr) Analogue de l'hirudine, methode de fabrication de celle-ci, composition anticoagulante et vecteur de secretion, microorganismes transformes contenant ledit vecteur et methode de fabrication de produits, produite a partir dudit microorganisme
CA2012025A1 (fr) Adn pour l'expression et la secretion
WO1990012098A3 (fr) Procedes et sequences d'acides nucleiques pour l'expression de l'operon de synthase de la cellulose
WO1996025498A3 (fr) Analogues de ligand mpl
SE9203084D0 (sv) A new enzyme and applications thereof
CA1174617A (fr) Synthese de proteines
KR910001046A (ko) 비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터
ATE145004T1 (de) Verfahren zur herstellung von luciferase durch rekombinante expression eines luciferase- kodierenden gens
DE3850456T2 (de) Klonierung des isoamylaseenzym kodierenden Gens und seine Verwendung zur Herstellung dieses Enzyms.
DE69211340D1 (de) Verfahren zur enzymatischen herstellung von makrolakton
El Akoum et al. Myxococcus xanthus produces an extracellular glycopeptide that displays blood anticoagulant properties
EP0818537A3 (fr) Procédé de clonage et de production de l'endonucléase SapI dans E. coli
HU204094B (en) Process for producing foreing protein in streptomyces

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed