CA1339800C - Process for recovering polypeptides localized in the periplasmic space of yeast - Google Patents

Process for recovering polypeptides localized in the periplasmic space of yeast

Info

Publication number
CA1339800C
CA1339800C CA000522859A CA522859A CA1339800C CA 1339800 C CA1339800 C CA 1339800C CA 000522859 A CA000522859 A CA 000522859A CA 522859 A CA522859 A CA 522859A CA 1339800 C CA1339800 C CA 1339800C
Authority
CA
Canada
Prior art keywords
process according
polyethoxylated
lysozyme
ionic surfactant
non ionic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA000522859A
Other languages
English (en)
French (fr)
Inventor
Annie J.F. De Batselier-Van Broekoven
Jacques Crahay
Jean M.A.G. Delcour
Jacques D.V. Hanotier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Labofina SA
Original Assignee
Labofina SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Labofina SA filed Critical Labofina SA
Application granted granted Critical
Publication of CA1339800C publication Critical patent/CA1339800C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CA000522859A 1985-11-13 1986-11-13 Process for recovering polypeptides localized in the periplasmic space of yeast Expired - Lifetime CA1339800C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BE903626 1985-11-13
BE85215849A BE903626A (fr) 1985-11-13 1985-11-13 Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.
BE0/215849 1985-11-13

Publications (1)

Publication Number Publication Date
CA1339800C true CA1339800C (en) 1998-04-07

Family

ID=25654770

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000522859A Expired - Lifetime CA1339800C (en) 1985-11-13 1986-11-13 Process for recovering polypeptides localized in the periplasmic space of yeast

Country Status (8)

Country Link
EP (1) EP0222725B1 (https=)
JP (1) JP2636844B2 (https=)
AT (1) ATE73490T1 (https=)
BE (1) BE903626A (https=)
CA (1) CA1339800C (https=)
DE (1) DE3684240D1 (https=)
ES (1) ES2030000T3 (https=)
GR (1) GR3004042T3 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE903626A (fr) * 1985-11-13 1986-03-03 Labofina Sa Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.
NO177065C (no) * 1988-09-26 1995-07-12 Labofina Sa Framgangsmåte for framstilling av enzymatisk aktivt humant lysozym
US7319021B2 (en) 2002-11-01 2008-01-15 Promega Corporation Cell lysis composition, methods of use, apparatus and kit
JP6778867B2 (ja) * 2015-08-07 2020-11-04 国立大学法人山口大学 ポリペプチド抽出方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63501121A (ja) * 1985-08-15 1988-04-28 アムジエン 酵母細胞からｂ型肝炎表面抗原を抽出するための細胞溶解法および緩衝液
BE903626A (fr) * 1985-11-13 1986-03-03 Labofina Sa Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.

Also Published As

Publication number Publication date
JP2636844B2 (ja) 1997-07-30
EP0222725A2 (en) 1987-05-20
ATE73490T1 (de) 1992-03-15
ES2030000T3 (es) 1992-10-16
EP0222725A3 (en) 1988-09-21
JPS62118896A (ja) 1987-05-30
GR3004042T3 (https=) 1993-03-31
EP0222725B1 (en) 1992-03-11
DE3684240D1 (de) 1992-04-16
BE903626A (fr) 1986-03-03

Similar Documents

Publication Publication Date Title
Bielicki et al. Recombinant human iduronate-2-sulphatase: correction of mucopolysaccharidosis-type II fibroblasts and characterization of the purified enzyme
Geremia et al. Molecular characterization of the proteinase‐encoding gene, prb1, related to mycoparasitism by Trichoderma harzianum
DK175460B1 (da) Fremgangsmåde til fremstilling af et pattedyrpolypeptid
JP2664860B2 (ja) 工業バチルス微生物種によるタンパク質又はポリペプチドの製造
DE3883041T2 (de) Stabile Genamplifizierung in prokaryotischer chromosonaler DNA.
Søgaard et al. Expression of cDNAs encoding barley α-amylase 1 and 2 in yeast and characterization of the secreted proteins
IZGÜ et al. Isolation and characterization of the K5-type yeast killer protein and its homology with an exo-β-1, 3-glucanase
Umile et al. A constitutive, plasma-membrane bound β-glucosidase in Trichoderma reesei
DD218118A5 (de) Verfahren zur herstellung eines hefe- oder nichthefepolypeptids
GB2138004A (en) A process for the production of a protein
Chen et al. Purification and partial characterization of the high and low molecular weight form (S-and F-form) of invertase secreted by Aspergillus nidulans
DE69636899T2 (de) Wirtszelle, die reduzierte mengen einer metalloprotease exprimiert und methoden zur verwendung dieser wirtszelle zur proteinproduktion
JP2633885B2 (ja) 新規な発現系
US5258502A (en) Immobilization and purification of fusion proteins using chitin-binding ability
EP0953644A1 (en) Systems for the mass production of proteins or peptides by microorganisms of the genus humicola
Arimatsu et al. Purification and properties of double-stranded RNA-degrading nuclease, dsRNase, from the digestive juice of the silkworm, Bombyx mori
CA1339800C (en) Process for recovering polypeptides localized in the periplasmic space of yeast
NO315379B1 (no) Isolert DNA-molekyl, hybridvektor, Aspergillus niger-stamme, Aspergillus niger-serin protease, fremgangsmåte for fremstilling av et önsketpolypeptid samtvert transformert med en hybrid ekspresjonvektor
US5124256A (en) Process for recovering polypeptides localized in the periplasmic space of yeast without breaking the cell wall by using an non-ionic detergent and a neutral salt
EP0362183B1 (en) Process for the production of human lysozyme
EP0205506B1 (en) A method of transforming fungi with a vector
EP0421919A2 (en) Polygalacturonase encoding fungal expression system
WO1999011797A1 (en) Gene
KR100618563B1 (ko) 셀룰로오스 결합 도메인을 이용한 효모에서 재조합단백질의 제조방법
Ali et al. Purification and characterization of a thermostable glucoamylase from a Myrothecium isolate

Legal Events

Date Code Title Description
MKEX Expiry

Effective date: 20150407