CA1296271C - Promoter arrangement for streptomycetes vectors - Google Patents
Promoter arrangement for streptomycetes vectorsInfo
- Publication number
- CA1296271C CA1296271C CA000520201A CA520201A CA1296271C CA 1296271 C CA1296271 C CA 1296271C CA 000520201 A CA000520201 A CA 000520201A CA 520201 A CA520201 A CA 520201A CA 1296271 C CA1296271 C CA 1296271C
- Authority
- CA
- Canada
- Prior art keywords
- plasmid
- promoters
- promoter
- fragment
- arrangement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000013598 vector Substances 0.000 title claims description 9
- 241001655322 Streptomycetales Species 0.000 title abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 17
- 239000013612 plasmid Substances 0.000 claims description 27
- 239000012634 fragment Substances 0.000 claims description 24
- 241000187747 Streptomyces Species 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 229950001790 tendamistat Drugs 0.000 claims description 4
- 108010037401 tendamistate Proteins 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 241000187398 Streptomyces lividans Species 0.000 claims 2
- 239000004382 Amylase Substances 0.000 description 4
- 229940122816 Amylase inhibitor Drugs 0.000 description 3
- 239000003392 amylase inhibitor Substances 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000828254 Streptomyces lividans TK24 Species 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 101100366892 Anopheles gambiae Stat gene Proteins 0.000 description 1
- 101100366894 Drosophila melanogaster Stat92E gene Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 101150099872 Stat gene Proteins 0.000 description 1
- NSFFHOGKXHRQEW-UHFFFAOYSA-N Thiostrepton B Natural products N1C(=O)C(C)NC(=O)C(=C)NC(=O)C(C)NC(=O)C(C(C)CC)NC(C(C2=N3)O)C=CC2=C(C(C)O)C=C3C(=O)OC(C)C(C=2SC=C(N=2)C2N=3)NC(=O)C(N=4)=CSC=4C(C(C)(O)C(C)O)NC(=O)C(N=4)CSC=4C(=CC)NC(=O)C(C(C)O)NC(=O)C(N=4)=CSC=4C21CCC=3C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-UHFFFAOYSA-N 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229940077844 iodine / potassium iodide Drugs 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000007775 late Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 229940070376 protein Drugs 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229930188070 thiostrepton Natural products 0.000 description 1
- NSFFHOGKXHRQEW-AIHSUZKVSA-N thiostrepton Chemical compound C([C@]12C=3SC=C(N=3)C(=O)N[C@H](C(=O)NC(/C=3SC[C@@H](N=3)C(=O)N[C@H](C=3SC=C(N=3)C(=O)N[C@H](C=3SC=C(N=3)[C@H]1N=1)[C@@H](C)OC(=O)C3=CC(=C4C=C[C@H]([C@@H](C4=N3)O)N[C@H](C(N[C@@H](C)C(=O)NC(=C)C(=O)N[C@@H](C)C(=O)N2)=O)[C@@H](C)CC)[C@H](C)O)[C@](C)(O)[C@@H](C)O)=C\C)[C@@H](C)O)CC=1C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-AIHSUZKVSA-N 0.000 description 1
- 229940063214 thiostrepton Drugs 0.000 description 1
- NSFFHOGKXHRQEW-OFMUQYBVSA-N thiostrepton A Natural products CC[C@H](C)[C@@H]1N[C@@H]2C=Cc3c(cc(nc3[C@H]2O)C(=O)O[C@H](C)[C@@H]4NC(=O)c5csc(n5)[C@@H](NC(=O)[C@H]6CSC(=N6)C(=CC)NC(=O)[C@@H](NC(=O)c7csc(n7)[C@]8(CCC(=N[C@@H]8c9csc4n9)c%10nc(cs%10)C(=O)NC(=C)C(=O)NC(=C)C(=O)N)NC(=O)[C@H](C)NC(=O)C(=C)NC(=O)[C@H](C)NC1=O)[C@@H](C)O)[C@](C)(O)[C@@H](C)O)[C@H](C)O NSFFHOGKXHRQEW-OFMUQYBVSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Electrotherapy Devices (AREA)
- Channel Selection Circuits, Automatic Tuning Circuits (AREA)
- Materials For Medical Uses (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3536182.4 | 1985-10-10 | ||
| DE19853536182 DE3536182A1 (de) | 1985-10-10 | 1985-10-10 | Promotor-anordnung fuer streptomyceten-vektoren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1296271C true CA1296271C (en) | 1992-02-25 |
Family
ID=6283273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000520201A Expired - Fee Related CA1296271C (en) | 1985-10-10 | 1986-10-09 | Promoter arrangement for streptomycetes vectors |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US4918007A (ja) |
| EP (1) | EP0218204B1 (ja) |
| JP (1) | JPS62100293A (ja) |
| AT (1) | ATE70306T1 (ja) |
| AU (1) | AU592825B2 (ja) |
| CA (1) | CA1296271C (ja) |
| DE (2) | DE3536182A1 (ja) |
| DK (1) | DK482686A (ja) |
| ES (1) | ES2038118T3 (ja) |
| FI (1) | FI91887C (ja) |
| GR (1) | GR3003950T3 (ja) |
| IE (1) | IE59590B1 (ja) |
| IL (1) | IL80250A (ja) |
| NO (1) | NO175319C (ja) |
| NZ (1) | NZ217842A (ja) |
| PT (1) | PT83519B (ja) |
| ZA (1) | ZA867697B (ja) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5426036A (en) * | 1987-05-05 | 1995-06-20 | Hoechst Aktiengesellschaft | Processes for the preparation of foreign proteins in streptomycetes |
| ZA929284B (en) * | 1991-12-12 | 1993-06-02 | Serono Lab | P0438, an new calcium-regulated promoter. |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2701890C2 (de) * | 1977-01-19 | 1983-08-04 | Hoechst Ag, 6230 Frankfurt | Peptielischer Glycosidhydrolase-Inhibitor aus einem Streptomyceten und seine Verwendung |
| ES8207217A1 (es) * | 1980-10-09 | 1982-09-01 | Hoechst Ag | Procedimiento para la preparacion de un inactivador de alfa-amilasa |
| CA1203185A (en) * | 1982-06-03 | 1986-04-15 | Thomas G. Eckhardt | Cloned streptomycete gene |
| US4717666A (en) * | 1984-03-05 | 1988-01-05 | Smithkline Beckman Corporation | Cloned streptomycete lividans excretable β-galactosidase gene |
| JPS5965099A (ja) * | 1982-10-05 | 1984-04-13 | Takeda Chem Ind Ltd | 発現用プロモ−タ−およびその用途 |
| DE3331860A1 (de) * | 1983-09-03 | 1985-03-21 | Hoechst Ag, 6230 Frankfurt | Verfahren zur herstellung von tendamistat |
| JPS60160887A (ja) * | 1984-02-02 | 1985-08-22 | Green Cross Corp:The | ベクタ− |
-
1985
- 1985-10-10 DE DE19853536182 patent/DE3536182A1/de not_active Withdrawn
-
1986
- 1986-10-02 ES ES198686113627T patent/ES2038118T3/es not_active Expired - Lifetime
- 1986-10-02 AT AT86113627T patent/ATE70306T1/de not_active IP Right Cessation
- 1986-10-02 DE DE8686113627T patent/DE3682861D1/de not_active Expired - Fee Related
- 1986-10-02 EP EP86113627A patent/EP0218204B1/de not_active Expired - Lifetime
- 1986-10-08 US US06/917,066 patent/US4918007A/en not_active Expired - Fee Related
- 1986-10-08 FI FI864071A patent/FI91887C/fi not_active IP Right Cessation
- 1986-10-08 NZ NZ217842A patent/NZ217842A/xx unknown
- 1986-10-08 IL IL80250A patent/IL80250A/xx not_active IP Right Cessation
- 1986-10-09 CA CA000520201A patent/CA1296271C/en not_active Expired - Fee Related
- 1986-10-09 AU AU63634/86A patent/AU592825B2/en not_active Ceased
- 1986-10-09 NO NO864020A patent/NO175319C/no unknown
- 1986-10-09 IE IE265886A patent/IE59590B1/en not_active IP Right Cessation
- 1986-10-09 JP JP61239432A patent/JPS62100293A/ja active Pending
- 1986-10-09 ZA ZA867697A patent/ZA867697B/xx unknown
- 1986-10-09 DK DK482686A patent/DK482686A/da not_active Application Discontinuation
- 1986-10-10 PT PT83519A patent/PT83519B/pt not_active IP Right Cessation
-
1992
- 1992-03-04 GR GR910402136T patent/GR3003950T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IE862658L (en) | 1987-04-10 |
| PT83519A (de) | 1986-11-01 |
| IE59590B1 (en) | 1994-03-09 |
| NO864020L (no) | 1987-04-13 |
| DE3682861D1 (de) | 1992-01-23 |
| IL80250A (en) | 1991-07-18 |
| EP0218204A3 (en) | 1988-09-07 |
| ES2038118T3 (es) | 1993-07-16 |
| EP0218204A2 (de) | 1987-04-15 |
| JPS62100293A (ja) | 1987-05-09 |
| GR3003950T3 (ja) | 1993-03-16 |
| EP0218204B1 (de) | 1991-12-11 |
| IL80250A0 (en) | 1987-01-30 |
| DK482686D0 (da) | 1986-10-09 |
| NO864020D0 (no) | 1986-10-09 |
| FI864071A (fi) | 1987-04-11 |
| PT83519B (pt) | 1989-05-31 |
| FI864071A0 (fi) | 1986-10-08 |
| ATE70306T1 (de) | 1991-12-15 |
| NZ217842A (en) | 1988-09-29 |
| AU592825B2 (en) | 1990-01-25 |
| AU6363486A (en) | 1987-04-16 |
| ZA867697B (en) | 1987-05-27 |
| US4918007A (en) | 1990-04-17 |
| NO175319B (no) | 1994-06-20 |
| DE3536182A1 (de) | 1987-04-16 |
| DK482686A (da) | 1987-04-11 |
| FI91887C (fi) | 1994-08-25 |
| FI91887B (fi) | 1994-05-13 |
| NO175319C (no) | 1994-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lévesque et al. | Diversity and relative strength of tandem promoters for the antibiotic-resistance genes of several integron | |
| US5266491A (en) | DNA fragment and expression plasmid containing the DNA fragment | |
| Kollek et al. | Isolation and characterization of the minimal fragment required for autonomous replication (“Basic replicon”) of a copy mutant (pKN 102) of the antibiotic resistance factor R1 | |
| Kodaira et al. | The dnaX gene encodes the DNA polymerase III holoenzyme τ subunit, precursor of the γ subunit, the dnaZ gene product | |
| HU205386B (en) | Process for expressing cloned lysostaphin gene and for producing dna fragment containing the gene, expression vector and transformed host cell | |
| Donohue et al. | Cloning and expression of the Rhodobacter sphaeroides reaction center H gene | |
| US6329172B1 (en) | ABC transporter gene cluster in pseudomonas fluorescens for enhanced lipase secretion | |
| Yasueda et al. | High-level direct expression of semi-synthetic human interleukin-6 in Escherichia coli and production of N-terminus met-free product | |
| Mizuno et al. | Cloning of the regulatory genes (ompR and envZ) for the matrix proteins of the Escherichia coli outer membrane | |
| CA1296271C (en) | Promoter arrangement for streptomycetes vectors | |
| JPH03503596A (ja) | 蛋白質若しくはポリペプチドの調製方法、該ポリペプチドをコードするｄｎａ配列、該ｄｎａ配列およびポリペプチドを有する微生物および該ポリペプチドの薬学的製剤としての利用 | |
| Saporito-Irwin et al. | Ammonium acetate protocol for the preparation of plasmid DNA suitable for mammalian cell transfections | |
| Mohr et al. | In vitro interactions of the histone-like protein IHF with the algD promoter, a critical site for control of mucoidy in Pseudomonas aeruginosa | |
| EP0187630B1 (en) | Cloned streptomyces beta-galactosidase promoter fragment | |
| Vasil et al. | Regulation of exotoxin A synthesis in Pseudomonas aeruginosa: characterization of toxA‐lacZ fusions in wild‐type and mutant strains | |
| EP0138644B1 (en) | Novel plasmid vectors | |
| AU689560B2 (en) | Method for stimulating profileration of colon cells using POMC-76-103 | |
| EP0182562B1 (en) | Process for a thermo-inducible gene expression in gram positive bacteria, bacillus subtilis strains containing plasmids which induce such an expression and portable vector systems | |
| Jun et al. | Nucleotide sequence and transcriptional start point of the kan gene encoding an aminoglycoside 3-N-acetyltransferase from Streptomyces griseus SS-1198PR | |
| JPH06253836A (ja) | 変異型の糖質加水分解酵素、該酵素の変異遺伝子及び該酵素を用いたオリゴ糖の製造方法 | |
| EP0590721A1 (en) | Method for expressing receptors of the human nervous system in the yeast Schizosaccharomyces pombe | |
| US6361982B1 (en) | Regulatory gene for clavulanic acid biosynthesis | |
| EP0286700A1 (en) | Biologically active recombinant human soluble Fc R-fragment, plasmids encoding this Fc R-fragment and the preparation thereof | |
| Rubin | Genetic analysis of the gentamicin resistance region of pPH1J1 and incorporation into a wide host range cloning vehicle | |
| KR920007684B1 (ko) | 신규한 발현 벡터와 그의 제조방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |