CA1256006A - Procedure for the detection of allergen-containing housedust - Google Patents
Procedure for the detection of allergen-containing housedustInfo
- Publication number
- CA1256006A CA1256006A CA000489187A CA489187A CA1256006A CA 1256006 A CA1256006 A CA 1256006A CA 000489187 A CA000489187 A CA 000489187A CA 489187 A CA489187 A CA 489187A CA 1256006 A CA1256006 A CA 1256006A
- Authority
- CA
- Canada
- Prior art keywords
- procedure
- solution
- dust
- color
- strongly alkaline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims abstract description 33
- 239000013566 allergen Substances 0.000 title claims abstract description 13
- 238000001514 detection method Methods 0.000 title abstract description 19
- 238000006243 chemical reaction Methods 0.000 claims abstract description 21
- 238000011161 development Methods 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 51
- 239000000428 dust Substances 0.000 claims description 41
- -1 aromatic diazo compound Chemical class 0.000 claims description 23
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 150000008044 alkali metal hydroxides Chemical class 0.000 claims description 11
- 238000005259 measurement Methods 0.000 claims description 11
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 10
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 7
- 241000238711 Pyroglyphidae Species 0.000 claims description 6
- 229940046533 house dust mites Drugs 0.000 claims description 6
- 239000000975 dye Substances 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 238000011088 calibration curve Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000012482 calibration solution Substances 0.000 claims description 2
- 230000036647 reaction Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 19
- 238000000605 extraction Methods 0.000 description 17
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 12
- 210000003608 fece Anatomy 0.000 description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- ZKZNWRUUTQNYTH-UHFFFAOYSA-N 4-azidobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=C(N=[N+]=[N-])C=C1 ZKZNWRUUTQNYTH-UHFFFAOYSA-N 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000000470 constituent Substances 0.000 description 6
- 241000238876 Acari Species 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 4
- 229960004784 allergens Drugs 0.000 description 4
- 206010020751 Hypersensitivity Diseases 0.000 description 3
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 3
- 230000007815 allergy Effects 0.000 description 3
- 239000011877 solvent mixture Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000010407 vacuum cleaning Methods 0.000 description 3
- ISPGCKRTUJLAFH-UHFFFAOYSA-N 4-azidobenzenesulfonic acid 4-diazocyclohexa-1,5-diene-1-sulfonic acid Chemical compound OS(=O)(=O)C1=CCC(=[N+]=[N-])C=C1.OS(=O)(=O)C1=CC=C(N=[N+]=[N-])C=C1 ISPGCKRTUJLAFH-UHFFFAOYSA-N 0.000 description 2
- 208000035533 House dust allergy Diseases 0.000 description 2
- 206010061217 Infestation Diseases 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000012496 blank sample Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 208000035285 Allergic Seasonal Rhinitis Diseases 0.000 description 1
- 235000019890 Amylum Nutrition 0.000 description 1
- 241000238740 Dermatophagoides pteronyssinus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 150000008049 diazo compounds Chemical class 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000007793 ph indicator Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plasma & Fusion (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Electrostatic Separation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3430896.2 | 1984-08-22 | ||
| DE19843430896 DE3430896A1 (de) | 1984-08-22 | 1984-08-22 | Verfahren zum nachweis von allergenhaltigem hausstaub |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1256006A true CA1256006A (en) | 1989-06-20 |
Family
ID=6243637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000489187A Expired CA1256006A (en) | 1984-08-22 | 1985-08-21 | Procedure for the detection of allergen-containing housedust |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0174448B1 (enrdf_load_stackoverflow) |
| JP (1) | JPS6159261A (enrdf_load_stackoverflow) |
| AT (1) | ATE41059T1 (enrdf_load_stackoverflow) |
| AU (1) | AU584916B2 (enrdf_load_stackoverflow) |
| CA (1) | CA1256006A (enrdf_load_stackoverflow) |
| DE (1) | DE3430896A1 (enrdf_load_stackoverflow) |
| DK (1) | DK377785A (enrdf_load_stackoverflow) |
| ES (1) | ES546303A0 (enrdf_load_stackoverflow) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4233565A1 (de) * | 1992-09-30 | 1993-06-17 | Crabiell Peter | Analyse- und reduzierungsverfahren von allergenen und deren ursachen |
| GB9913487D0 (en) * | 1999-06-11 | 1999-08-11 | Pirzad Ramin | Dust mite detection |
| DE10327327A1 (de) * | 2003-06-16 | 2005-01-27 | Hofmann, Frieder, Dipl.-Biol. | Verfahren zum Aufbereiten einer aus Partikeln bestehenden Probe |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2502786B1 (fr) * | 1981-03-24 | 1985-06-21 | Stallergenes Laboratoire | Procede de fixation d'antigenes et d'anticorps sur support de polysaccharides, et utilisation du produit ainsi obtenu pour les dosages immunologiques |
| DE3344087C1 (de) * | 1983-12-06 | 1985-04-11 | Werner & Mertz Gmbh, 6500 Mainz | Verfahren zum Nachweis von allergenhaltigem Hausstaub |
| DE3404821A1 (de) * | 1984-02-10 | 1985-08-14 | Werner & Mertz Gmbh, 6500 Mainz | Verfahren zum nachweis von allergenhaltigem hausstaub |
| JPS6435293A (en) * | 1987-07-30 | 1989-02-06 | Nippon Butsuri Tanko Kk | Wave motion generating method and device therefor |
-
1984
- 1984-08-22 DE DE19843430896 patent/DE3430896A1/de active Granted
-
1985
- 1985-06-19 EP EP85107570A patent/EP0174448B1/de not_active Expired
- 1985-06-19 AT AT85107570T patent/ATE41059T1/de not_active IP Right Cessation
- 1985-08-20 AU AU46477/85A patent/AU584916B2/en not_active Expired
- 1985-08-20 DK DK377785A patent/DK377785A/da not_active Application Discontinuation
- 1985-08-21 ES ES546303A patent/ES546303A0/es active Granted
- 1985-08-21 CA CA000489187A patent/CA1256006A/en not_active Expired
- 1985-08-21 JP JP60181928A patent/JPS6159261A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| AU584916B2 (en) | 1989-06-08 |
| EP0174448A2 (de) | 1986-03-19 |
| JPS6159261A (ja) | 1986-03-26 |
| DE3430896A1 (de) | 1986-03-06 |
| DK377785A (da) | 1986-02-23 |
| ES8700757A2 (es) | 1986-10-16 |
| EP0174448B1 (de) | 1989-03-01 |
| EP0174448A3 (en) | 1986-03-26 |
| AU4647785A (en) | 1986-02-27 |
| ATE41059T1 (de) | 1989-03-15 |
| JPH0464591B2 (enrdf_load_stackoverflow) | 1992-10-15 |
| DE3430896C2 (enrdf_load_stackoverflow) | 1989-05-18 |
| ES546303A0 (es) | 1986-10-16 |
| DK377785D0 (da) | 1985-08-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |