CA1180274A - Immunoassay of proteins - Google Patents
Immunoassay of proteinsInfo
- Publication number
- CA1180274A CA1180274A CA000389619A CA389619A CA1180274A CA 1180274 A CA1180274 A CA 1180274A CA 000389619 A CA000389619 A CA 000389619A CA 389619 A CA389619 A CA 389619A CA 1180274 A CA1180274 A CA 1180274A
- Authority
- CA
- Canada
- Prior art keywords
- protein
- fragment
- assayed
- distinctive
- proteins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 124
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 124
- 238000003018 immunoassay Methods 0.000 title claims abstract description 9
- 239000012634 fragment Substances 0.000 claims abstract description 100
- 238000003556 assay Methods 0.000 claims abstract description 62
- 229940111202 pepsin Drugs 0.000 claims abstract description 29
- 102000057297 Pepsin A Human genes 0.000 claims abstract description 28
- 108090000284 Pepsin A Proteins 0.000 claims abstract description 28
- 210000002966 serum Anatomy 0.000 claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 229940088598 enzyme Drugs 0.000 claims abstract description 19
- 239000013060 biological fluid Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 69
- 230000029087 digestion Effects 0.000 claims description 40
- 239000002245 particle Substances 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 15
- 230000004520 agglutination Effects 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 239000013566 allergen Substances 0.000 claims description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 6
- 229940070376 protein Drugs 0.000 claims description 5
- 230000006862 enzymatic digestion Effects 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 101100519432 Rattus norvegicus Pdzd2 gene Proteins 0.000 claims description 2
- 102000004142 Trypsin Human genes 0.000 claims description 2
- 108090000631 Trypsin Proteins 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000012588 trypsin Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 238000001976 enzyme digestion Methods 0.000 abstract description 8
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 230000000295 complement effect Effects 0.000 abstract description 5
- 239000012530 fluid Substances 0.000 abstract description 4
- 230000013777 protein digestion Effects 0.000 abstract description 4
- 235000018102 proteins Nutrition 0.000 description 90
- 230000000890 antigenic effect Effects 0.000 description 25
- 239000004816 latex Substances 0.000 description 25
- 229920000126 latex Polymers 0.000 description 25
- 102000011923 Thyrotropin Human genes 0.000 description 12
- 108010061174 Thyrotropin Proteins 0.000 description 12
- 239000004365 Protease Substances 0.000 description 11
- 230000002452 interceptive effect Effects 0.000 description 11
- 102000036639 antigens Human genes 0.000 description 10
- 108091007433 antigens Proteins 0.000 description 10
- 102000035195 Peptidases Human genes 0.000 description 9
- 108091005804 Peptidases Proteins 0.000 description 9
- 108090000765 processed proteins & peptides Proteins 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 108010017384 Blood Proteins Proteins 0.000 description 6
- 102000004506 Blood Proteins Human genes 0.000 description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 208000026935 allergic disease Diseases 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 235000019419 proteases Nutrition 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 239000007975 buffered saline Substances 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 3
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 3
- 108010000521 Human Growth Hormone Proteins 0.000 description 3
- 102000002265 Human Growth Hormone Human genes 0.000 description 3
- 239000000854 Human Growth Hormone Substances 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 210000000981 epithelium Anatomy 0.000 description 3
- 229940028334 follicle stimulating hormone Drugs 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000004850 protein–protein interaction Effects 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 108090000526 Papain Proteins 0.000 description 2
- 102000004576 Placental Lactogen Human genes 0.000 description 2
- 108010003044 Placental Lactogen Proteins 0.000 description 2
- 239000000381 Placental Lactogen Substances 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 239000008351 acetate buffer Substances 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 239000001166 ammonium sulphate Substances 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000003196 chaotropic effect Effects 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 229940055729 papain Drugs 0.000 description 2
- 235000019834 papain Nutrition 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- CUKWUWBLQQDQAC-VEQWQPCFSA-N (3s)-3-amino-4-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s,3s)-1-[[(2s)-1-[(2s)-2-[[(1s)-1-carboxyethyl]carbamoyl]pyrrolidin-1-yl]-3-(1h-imidazol-5-yl)-1-oxopropan-2-yl]amino]-3-methyl-1-oxopentan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxopropan-2-yl]amino]-3-methyl-1-ox Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(O)=O)C(C)C)C1=CC=C(O)C=C1 CUKWUWBLQQDQAC-VEQWQPCFSA-N 0.000 description 1
- AJDIZQLSFPQPEY-UHFFFAOYSA-N 1,1,2-Trichlorotrifluoroethane Chemical compound FC(F)(Cl)C(F)(Cl)Cl AJDIZQLSFPQPEY-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- BMYNFMYTOJXKLE-UHFFFAOYSA-N 3-azaniumyl-2-hydroxypropanoate Chemical compound NCC(O)C(O)=O BMYNFMYTOJXKLE-UHFFFAOYSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 102400000344 Angiotensin-1 Human genes 0.000 description 1
- 101800000734 Angiotensin-1 Proteins 0.000 description 1
- 102400000345 Angiotensin-2 Human genes 0.000 description 1
- 101800000733 Angiotensin-2 Proteins 0.000 description 1
- 235000004355 Artemisia lactiflora Nutrition 0.000 description 1
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 1
- 240000006891 Artemisia vulgaris Species 0.000 description 1
- 241001225321 Aspergillus fumigatus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001149956 Cladosporium herbarum Species 0.000 description 1
- 241000518994 Conta Species 0.000 description 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- 240000004585 Dactylis glomerata Species 0.000 description 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 101000891982 Homo sapiens Alpha-crystallin B chain Proteins 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 102000018071 Immunoglobulin Fc Fragments Human genes 0.000 description 1
- 108010091135 Immunoglobulin Fc Fragments Proteins 0.000 description 1
- 208000001019 Inborn Errors Metabolism Diseases 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 241000746983 Phleum pratense Species 0.000 description 1
- 244000239204 Plantago lanceolata Species 0.000 description 1
- 235000010503 Plantago lanceolata Nutrition 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010071690 Prealbumin Proteins 0.000 description 1
- 102000007584 Prealbumin Human genes 0.000 description 1
- 101710149934 Protein tas Proteins 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- ORWYRWWVDCYOMK-HBZPZAIKSA-N angiotensin I Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(O)=O)C(C)C)C1=CC=C(O)C=C1 ORWYRWWVDCYOMK-HBZPZAIKSA-N 0.000 description 1
- 229940091771 aspergillus fumigatus Drugs 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004182 chemical digestion Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229960001760 dimethyl sulfoxide Drugs 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 102000045069 human CRYAB Human genes 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000016245 inborn errors of metabolism Diseases 0.000 description 1
- 208000015978 inherited metabolic disease Diseases 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 230000001592 luteinising effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- VOVZXURTCKPRDQ-CQSZACIVSA-N n-[4-[chloro(difluoro)methoxy]phenyl]-6-[(3r)-3-hydroxypyrrolidin-1-yl]-5-(1h-pyrazol-5-yl)pyridine-3-carboxamide Chemical compound C1[C@H](O)CCN1C1=NC=C(C(=O)NC=2C=CC(OC(F)(F)Cl)=CC=2)C=C1C1=CC=NN1 VOVZXURTCKPRDQ-CQSZACIVSA-N 0.000 description 1
- 229940043515 other immunoglobulins in atc Drugs 0.000 description 1
- -1 over 50 Chemical class 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000001817 pituitary effect Effects 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8035794 | 1980-11-07 | ||
| GB8035794 | 1980-11-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1180274A true CA1180274A (en) | 1985-01-02 |
Family
ID=10517154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000389619A Expired CA1180274A (en) | 1980-11-07 | 1981-11-06 | Immunoassay of proteins |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4455381A (OSRAM) |
| EP (1) | EP0051985B2 (OSRAM) |
| JP (1) | JPS57108666A (OSRAM) |
| AU (1) | AU548783B2 (OSRAM) |
| CA (1) | CA1180274A (OSRAM) |
| DE (1) | DE3168691D1 (OSRAM) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0083869B1 (en) * | 1982-01-05 | 1985-10-16 | International Institute Of Cellular And Molecular Pathology (Icp) | Method of immunoassay |
| DE3366740D1 (en) * | 1982-08-06 | 1986-11-13 | Int Inst Cellular Molecul Path | Particle agglutination assay of antigens |
| US4539292A (en) * | 1982-12-29 | 1985-09-03 | Reid Michael J | Immunoassay technique for specific immunoglobulin E |
| US4650751A (en) * | 1983-04-29 | 1987-03-17 | Technicon Instruments Corporation | Protected binding assay avoiding non-specific protein interference |
| FR2549230B1 (fr) * | 1983-06-14 | 1986-04-11 | Bio Merieux | Procede de detection d'un oligomere dans un milieu liquide contenant des monomeres correspondants, dispositif pour la mise en oeuvre de ce procede et application notamment a la recherche d'allergenes |
| US4668620A (en) * | 1984-02-22 | 1987-05-26 | Syntex (U.S.A.) Inc. | Reducing background interference activity in enzyme-label immunoassays |
| BE899664A (fr) * | 1984-05-15 | 1984-08-31 | Inst Internat De Pathologie Ce | Procede de dosage immunologique d'une substance dans un echantillon liquide. |
| DE3674567D1 (de) * | 1985-03-18 | 1990-10-31 | Royal Free Hosp School Med | Verbesserungen in bezug auf nachweis von viren und antikoerpern. |
| US5242802A (en) * | 1985-03-29 | 1993-09-07 | Hybritech Incorporated | Processes for the stabilization of prostate specific antigen in natural matrices |
| GB8621495D0 (en) * | 1986-09-05 | 1986-10-15 | Acade Diagnostic Systems Sa Nv | Assay |
| JP2523171B2 (ja) * | 1987-08-12 | 1996-08-07 | 帝人株式会社 | 免疫測定方法及びそれに用いる試薬キット |
| TW517059B (en) * | 1994-07-25 | 2003-01-11 | Ciba Geigy Ag | New process for the production of biologically active protein |
| US6066464A (en) * | 1996-12-10 | 2000-05-23 | Diagnostic Systems Laboratories, Inc. | Immunoassay of IGF family of peptides, their binding proteins and related molecules in dried whole blood filter paper spots |
| ES2198645T3 (es) * | 1998-10-15 | 2004-02-01 | Halmon Laboratoria Beheer B.V. | Procedimiento mejorado para diagnosticos de alergia. |
| GB9827845D0 (en) * | 1998-12-17 | 1999-02-10 | Scolab S A | Immunoassay or proteins |
| US7560238B2 (en) * | 2000-05-30 | 2009-07-14 | Mitsubishi Kagaku Iatron, Inc. | Immunological latex turbidimetry method and reagent therefor |
| US7402423B2 (en) * | 2004-08-13 | 2008-07-22 | Biomed Solutions, Llc | Apparatus for the detection of pepsin |
| US7422866B2 (en) * | 2005-08-10 | 2008-09-09 | Agilent Technologies, Inc. | On-line enzymatic digestion in separation-detection methods |
| US20070077546A1 (en) * | 2005-08-10 | 2007-04-05 | Zhenghua Ji | Concentration techniques in chromatographic separation |
| EP2131195B1 (en) | 2005-10-12 | 2012-04-25 | Allergan, Inc. | Assays of molecular or subcellular proximity using depolarization after resonance energy transfer (DARET) |
| WO2025047641A1 (ja) * | 2023-08-25 | 2025-03-06 | 積水メディカル株式会社 | 免疫学的測定方法、免疫学的測定用試薬、免疫学的測定用検体前処理液、及び免疫学的測定用試薬キット、非特異反応抑制剤 |
| JP7736395B1 (ja) * | 2024-02-29 | 2025-09-09 | 積水メディカル株式会社 | 免疫学的測定方法、免疫学的測定用試薬、免疫学的測定用検体前処理液、免疫学的測定用試薬キット、及び非特異反応抑制剤 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3962039A (en) * | 1974-08-08 | 1976-06-08 | Center For Laboratory Medicine | Analytical procedure for thyroid hormones |
| JPS5236091A (en) * | 1975-09-15 | 1977-03-19 | Abbott Lab | Analytical method using digestive enzyme for serum connective protein |
| DE2718700A1 (de) * | 1977-04-27 | 1978-11-02 | Hans A Dipl Chem Dr Thoma | Verfahren zur gesamtbestimmung von hormonen und pharmaka |
| ES471585A1 (es) * | 1977-07-15 | 1979-01-16 | Behringwerke Ag | Procedimiento para la determinacion de reactivos fc |
| US4211762A (en) * | 1978-01-26 | 1980-07-08 | Huggins Keith G | Specific binding assay techniques |
| GB2013688B (en) * | 1978-01-26 | 1982-06-30 | Technicon Instr | Insolubilised proteins and immunoassays utilising them |
| GB2013211B (en) * | 1978-01-26 | 1982-06-30 | Technicon Instr | Immunoassays using f(ab')2 fragments |
-
1981
- 1981-11-05 EP EP81305261A patent/EP0051985B2/en not_active Expired
- 1981-11-05 DE DE8181305261T patent/DE3168691D1/de not_active Expired
- 1981-11-06 AU AU77162/81A patent/AU548783B2/en not_active Ceased
- 1981-11-06 CA CA000389619A patent/CA1180274A/en not_active Expired
- 1981-11-06 US US06/319,023 patent/US4455381A/en not_active Expired - Lifetime
- 1981-11-07 JP JP56177847A patent/JPS57108666A/ja active Granted
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| Publication number | Publication date |
|---|---|
| AU7716281A (en) | 1982-05-13 |
| AU548783B2 (en) | 1986-01-02 |
| DE3168691D1 (en) | 1985-03-14 |
| US4455381A (en) | 1984-06-19 |
| EP0051985A3 (en) | 1982-08-11 |
| EP0051985A2 (en) | 1982-05-19 |
| EP0051985B2 (en) | 1989-12-27 |
| JPH0221548B2 (OSRAM) | 1990-05-15 |
| JPS57108666A (en) | 1982-07-06 |
| EP0051985B1 (en) | 1985-01-30 |
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