CA1170475A - Sample support as part of a photometric or fluorometric apparatus - Google Patents
Sample support as part of a photometric or fluorometric apparatusInfo
- Publication number
- CA1170475A CA1170475A CA000403781A CA403781A CA1170475A CA 1170475 A CA1170475 A CA 1170475A CA 000403781 A CA000403781 A CA 000403781A CA 403781 A CA403781 A CA 403781A CA 1170475 A CA1170475 A CA 1170475A
- Authority
- CA
- Canada
- Prior art keywords
- radiation
- block unit
- sample
- sample support
- filters
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
- G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Abstract of the Invention The invention relates to a sample support as part of a photometric or fluorometric apparatus. According to the prior art two separate devices are required. Modification to convert a photometer into a fluorimeter and vice versa is laborious. This has resulted in the development of many different designs of both photometric and fluorometric apparatus specifically for one object. The aim of the invention is therefore to provide a sample support which enables virtually any of the investigations in question to be carried out if required - independently of the type of basic apparatus used - by simple manipulation or modification. The solution according to the invention provides a sample support in the form of a block unit which is intended to receive cuvettes or test strips and is fitted with slide-in holders for different filters and contains a reflectance deflector which guides the light or fluorescent radiation to the detector. By the simple conversion of standard commercial apparatus it is possible to modify the actual specifications of the basic apparatus so that a broader spread of different analyses can be carried out in substantially standard basic apparatus.
Description
~i 7~4 t 5~
The invention relates to a sample support as part of a photometric or fluorometric apparatus, designed to receive samples which aré to be examined, by measurement of their absorption of light or radlation, whilst for carrying out the fluorescence process at least one filter for excitation or one filter for resolution of the radiation is provided as a direct component of the fluorimeter.
Basically, there are photometers and fluorimeters for measuring absorbances and tramsmittances, in the form of single and double beam apparatus.
More specifically, a distinction is drawn between photometers for examining liquid substances and reflection photometers for examining test strips as special developments and reflection fluorimeters for examining liquid sub-stances and reflection fluorimeters for examining test strips as special developments.
In the case of certain designs made by individual firms it is possible to convert photometers into fluorimeters. However, measurements of reflectance cannot be carried out with a normal photometer without major conversion.
For this reason, reflectance or fluorescent test strips are in-creasingly being offered for sale by the chemical industry in conjunction with their own products and adapted to these products.
This has resulted in a large number of apparatus which can be used only Eor one type of application; admittedly, they can be bought relatively cheaply, by being tied to a particular product, but conversely, if development continues alon~ these lines, the result is a plurality of apparatus which are not used to the optimum and which further have to be divided into two main groups, namely cuvette supports and test strip supports. Furthermore, it should be added that the one-sided linking of the apparatus with specific products of the chemical or pharmaceutical industry does not always ensure a ~7~)~75 supply of apparatus intended for use in accurate investigations.
In connection with this, it should be pointed out, for example, that fluorimeters are substantially more accurate than simple photometers but, conversely, fluorimeters are, at least in some cases, more sensitive or delicate.
The aim of this invention is therefore to provide a sample support of the kind described hereinbefore which can be used both with a photometer and with a fluorimeter and which can be adapted to the particular investigations to be carried out - independently of the basic type of apparatus used - by simple manipulation, without the need for any major conversion.
In the solu-tion to this problem according to the invention, the sample supports in each case form an optical block unit of the same size and the block unit is selectively constructed to receive a cuvette or a test strip as the sample for measurement, whilst a total shutter is mounted behind the sample for measurement, in the direction of the incoming radiation; the light or fluorescent radiation which is to be scattered by or emitted from the sample to be investigated, generally at an angle of 90 , can be guided into the receiver in the direction of the incoming radiation by means of a further multiple optical reflectance deflecting means; and the block unit is constructed with at least one holder into which may be slid excitation or resolution filters of uniform dimensions for measuring the absorption of fluorescent radiation, or, in some cases, normal- or quartz-glass slides for measuring light absorption.
~y simply modifying commercially available apparatus in this way, it is possible to alter the basic specification of the apparatus, so that a broader spread of analyses can be carried out with essentially standard basic apparatus.
This is particularly important in view of the increasingly wider use of reElectance and fluorescence test strips. The reflectance deflecting means to be provided in the optical block unit may consist of suitably arranged flat mirrors, concave mirrors or prisms, arranged to take up the minimum space, so that, as a rule, the block unit is the same size as a standard commercial cuvette or test strip guide used in clinical chemistry, or is suitably dimensioned for the particular requirements, i.e.
the size of the cuvette or test strip.
Since the standard commercial measuring apparatus already contain a filter, it is proposed that the block unit should comprise at least one or more slide-in holders for filters, both in the direction of entry of radiation and also in the direction of exit of the radiation.
Depending on the particular construction, the missing filter is then inserted at the radiation entry or exit of the block.
The invention is described more fully with reference to the accompanying schematic drawing in which:
Figure 1 shows a block unit intended to receive a cuvette and for use with a fluorimeter;
Figure 2 shows a block unit intended to receive a test strip, for use with a fluorimeter;
Figure 3 shows a block unit intended to receive a solid strip, for use with a reflection photometer; and Figure 4 shows an example of the arrangement of block units with different inserts, being used with a fluorimeter, in a multiple sample support.
The sample of material for measurement - which is located either in a cuvette or on a test strip - is placed in the optical block unit 2 and inserted into the photometer or fluorimeter 1.
When the fluorescence process is carried out, the radiation is passed into the block unit 2 through an excitation filter 3 mounted in the path of the beam and is generally guided through a radiation resolving filter 4 1~1L'7~475 mounted in a slide-in holder 5 in the block unit 2, and so guided on to the sample 6 or 7. The excitation radiation 12 entering the block unit 2 in the direction of the radiation entry is prevented from going any further in this direction by means of a total shutter 8 mounted behind the sample 6 or 7. The fluorescent radiation 12' leaving the sample 6 or 7 and usually offset by 90 to the direction of the excitation radiation 12 is deflected towards the radiation exit from the block unit 2 by the deflector means 9 which consists of a succession of mirrors, prisms, or the like, and after first passing through a fluorescent radiation resolving filter 4' mounted in another slide-in holder 5, the said radiation leaves the block unit 2 and travels towards the receiver or detector 10, which is connected to an indicator 11.
Figure 1 shows a f]uorimeter 1 with a cuvette 6 inserted therein and Figure 2 shows a fluorimeter 1 with a test strip 7 inserted. The two block units 2 are, moreover, of fundamentally uniform construction.
Figure 3 shows the same block unit as Figure 2, but in this case it is associated with a photometer 1, with the result that the excitation filter 3 and the resolving filters 4 and 4' are omitted; the latter may be replaced by quartz glass slides.
Figure 4 shows a sample carrier which is formed by a peripherally mounted rotary plate 13 for receiving block units 2 and a concentrically mounted, separately rotatable ring insert 14 for receiving different filter systems, if required. Inside the ring 14 is mounted the receiver 10, including the indicator 11, whilst outside the suppOrt system 13/14 is mounted the excitation filter 3 required in the fluorescence process.
, '
The invention relates to a sample support as part of a photometric or fluorometric apparatus, designed to receive samples which aré to be examined, by measurement of their absorption of light or radlation, whilst for carrying out the fluorescence process at least one filter for excitation or one filter for resolution of the radiation is provided as a direct component of the fluorimeter.
Basically, there are photometers and fluorimeters for measuring absorbances and tramsmittances, in the form of single and double beam apparatus.
More specifically, a distinction is drawn between photometers for examining liquid substances and reflection photometers for examining test strips as special developments and reflection fluorimeters for examining liquid sub-stances and reflection fluorimeters for examining test strips as special developments.
In the case of certain designs made by individual firms it is possible to convert photometers into fluorimeters. However, measurements of reflectance cannot be carried out with a normal photometer without major conversion.
For this reason, reflectance or fluorescent test strips are in-creasingly being offered for sale by the chemical industry in conjunction with their own products and adapted to these products.
This has resulted in a large number of apparatus which can be used only Eor one type of application; admittedly, they can be bought relatively cheaply, by being tied to a particular product, but conversely, if development continues alon~ these lines, the result is a plurality of apparatus which are not used to the optimum and which further have to be divided into two main groups, namely cuvette supports and test strip supports. Furthermore, it should be added that the one-sided linking of the apparatus with specific products of the chemical or pharmaceutical industry does not always ensure a ~7~)~75 supply of apparatus intended for use in accurate investigations.
In connection with this, it should be pointed out, for example, that fluorimeters are substantially more accurate than simple photometers but, conversely, fluorimeters are, at least in some cases, more sensitive or delicate.
The aim of this invention is therefore to provide a sample support of the kind described hereinbefore which can be used both with a photometer and with a fluorimeter and which can be adapted to the particular investigations to be carried out - independently of the basic type of apparatus used - by simple manipulation, without the need for any major conversion.
In the solu-tion to this problem according to the invention, the sample supports in each case form an optical block unit of the same size and the block unit is selectively constructed to receive a cuvette or a test strip as the sample for measurement, whilst a total shutter is mounted behind the sample for measurement, in the direction of the incoming radiation; the light or fluorescent radiation which is to be scattered by or emitted from the sample to be investigated, generally at an angle of 90 , can be guided into the receiver in the direction of the incoming radiation by means of a further multiple optical reflectance deflecting means; and the block unit is constructed with at least one holder into which may be slid excitation or resolution filters of uniform dimensions for measuring the absorption of fluorescent radiation, or, in some cases, normal- or quartz-glass slides for measuring light absorption.
~y simply modifying commercially available apparatus in this way, it is possible to alter the basic specification of the apparatus, so that a broader spread of analyses can be carried out with essentially standard basic apparatus.
This is particularly important in view of the increasingly wider use of reElectance and fluorescence test strips. The reflectance deflecting means to be provided in the optical block unit may consist of suitably arranged flat mirrors, concave mirrors or prisms, arranged to take up the minimum space, so that, as a rule, the block unit is the same size as a standard commercial cuvette or test strip guide used in clinical chemistry, or is suitably dimensioned for the particular requirements, i.e.
the size of the cuvette or test strip.
Since the standard commercial measuring apparatus already contain a filter, it is proposed that the block unit should comprise at least one or more slide-in holders for filters, both in the direction of entry of radiation and also in the direction of exit of the radiation.
Depending on the particular construction, the missing filter is then inserted at the radiation entry or exit of the block.
The invention is described more fully with reference to the accompanying schematic drawing in which:
Figure 1 shows a block unit intended to receive a cuvette and for use with a fluorimeter;
Figure 2 shows a block unit intended to receive a test strip, for use with a fluorimeter;
Figure 3 shows a block unit intended to receive a solid strip, for use with a reflection photometer; and Figure 4 shows an example of the arrangement of block units with different inserts, being used with a fluorimeter, in a multiple sample support.
The sample of material for measurement - which is located either in a cuvette or on a test strip - is placed in the optical block unit 2 and inserted into the photometer or fluorimeter 1.
When the fluorescence process is carried out, the radiation is passed into the block unit 2 through an excitation filter 3 mounted in the path of the beam and is generally guided through a radiation resolving filter 4 1~1L'7~475 mounted in a slide-in holder 5 in the block unit 2, and so guided on to the sample 6 or 7. The excitation radiation 12 entering the block unit 2 in the direction of the radiation entry is prevented from going any further in this direction by means of a total shutter 8 mounted behind the sample 6 or 7. The fluorescent radiation 12' leaving the sample 6 or 7 and usually offset by 90 to the direction of the excitation radiation 12 is deflected towards the radiation exit from the block unit 2 by the deflector means 9 which consists of a succession of mirrors, prisms, or the like, and after first passing through a fluorescent radiation resolving filter 4' mounted in another slide-in holder 5, the said radiation leaves the block unit 2 and travels towards the receiver or detector 10, which is connected to an indicator 11.
Figure 1 shows a f]uorimeter 1 with a cuvette 6 inserted therein and Figure 2 shows a fluorimeter 1 with a test strip 7 inserted. The two block units 2 are, moreover, of fundamentally uniform construction.
Figure 3 shows the same block unit as Figure 2, but in this case it is associated with a photometer 1, with the result that the excitation filter 3 and the resolving filters 4 and 4' are omitted; the latter may be replaced by quartz glass slides.
Figure 4 shows a sample carrier which is formed by a peripherally mounted rotary plate 13 for receiving block units 2 and a concentrically mounted, separately rotatable ring insert 14 for receiving different filter systems, if required. Inside the ring 14 is mounted the receiver 10, including the indicator 11, whilst outside the suppOrt system 13/14 is mounted the excitation filter 3 required in the fluorescence process.
, '
Claims (3)
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. Sample support as part of a photometric or fluorometric apparatus, designed to receive samples which are to be examined by the measurement of their absorption of light or radiation, whilst for carrying out the fluorescence process at least one filter for excitation or one filter for resolution of the radiation is provided as a direct component of the fluorimeter, characterised in that the sample supports in each case form an optical block unit of the same size and the block unit is selectively constructed to receive a cuvette or a test strip as the sample for measure-ment, whilst a total shutter is mounted behind the sample for measurement, in the direction of entry of radiation; in that the light or fluorescent radiation which is to be scattered by or emitted from the sample to be investigated, generally at an angle of 90°, can be guided into the receiver in the direction of the incoming radiation by means of a further multiple optical reflectance deflecting means; and in that the block unit is constructed with at least one holder into which may be slid excitation filters or resolution filters of uniform dimensions for measuring the absorption of fluorescent radiation, or, in some cases, normal- or quartz-glass slides for measuring light absorption.
2. Sample support as claimed in claim 1, characterised in that the block unit comprises at least one or more slide-in holders for filters both in the direction of entry of the radiation and also in the direction of exit of the radiation.
3. Sample support as claimed in claim 1 characterised in that the holders in block unit are so positioned that filters therein will lie across the path of the incoming or outgoing radiation.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3121060.0 | 1981-05-27 | ||
| DE3121060A DE3121060C2 (en) | 1981-05-27 | 1981-05-27 | "Slides as part of a photometer or fluorometer device" |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1170475A true CA1170475A (en) | 1984-07-10 |
Family
ID=6133318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000403781A Expired CA1170475A (en) | 1981-05-27 | 1982-05-26 | Sample support as part of a photometric or fluorometric apparatus |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0067963A3 (en) |
| JP (1) | JPS57199941A (en) |
| CA (1) | CA1170475A (en) |
| DE (1) | DE3121060C2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0197425A3 (en) * | 1985-04-11 | 1988-07-13 | Abbott Laboratories | Nephelometric analyzer |
| DE4440119A1 (en) * | 1994-11-10 | 1996-05-15 | Tzn Forschung & Entwicklung | Measuring device for checking gray filters |
| WO2021170364A1 (en) * | 2020-02-26 | 2021-09-02 | Robert Bosch Gmbh | An optical filter holder for a bio-analyte device |
| CN114047170B (en) * | 2021-11-27 | 2022-08-16 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN118243918A (en) * | 2024-05-28 | 2024-06-25 | 南京佰抗生物科技有限公司 | Light path module for dry fluorescent immunodetection |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3800161A (en) * | 1972-12-19 | 1974-03-26 | Atomic Energy Commission | Portable dynamic multistation photometer-fluorometer |
| US4097735A (en) * | 1977-05-24 | 1978-06-27 | Corning Glass Works | Testing the operation of a recording fluorometer/densitometer |
| DE2938056C2 (en) * | 1979-09-20 | 1986-12-11 | Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München | Device for the fluorometric analysis of samples |
| JPS5772046A (en) * | 1980-10-23 | 1982-05-06 | Dainippon Screen Mfg Co Ltd | Transmission type concentration gauge |
-
1981
- 1981-05-27 DE DE3121060A patent/DE3121060C2/en not_active Expired
-
1982
- 1982-05-17 EP EP82104298A patent/EP0067963A3/en not_active Withdrawn
- 1982-05-26 JP JP57089596A patent/JPS57199941A/en active Pending
- 1982-05-26 CA CA000403781A patent/CA1170475A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DE3121060A1 (en) | 1982-12-23 |
| EP0067963A2 (en) | 1982-12-29 |
| JPS57199941A (en) | 1982-12-08 |
| DE3121060C2 (en) | 1983-09-22 |
| EP0067963A3 (en) | 1983-11-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |