CA1154366A - Enzymatic method and stabilized solutions for determining total cholesterol in human serum - Google Patents
Enzymatic method and stabilized solutions for determining total cholesterol in human serumInfo
- Publication number
- CA1154366A CA1154366A CA000357900A CA357900A CA1154366A CA 1154366 A CA1154366 A CA 1154366A CA 000357900 A CA000357900 A CA 000357900A CA 357900 A CA357900 A CA 357900A CA 1154366 A CA1154366 A CA 1154366A
- Authority
- CA
- Canada
- Prior art keywords
- solution
- cholesterol
- recited
- buffer solution
- esterase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title claims abstract description 233
- 235000012000 cholesterol Nutrition 0.000 title claims abstract description 114
- 210000002966 serum Anatomy 0.000 title abstract description 45
- 238000006911 enzymatic reaction Methods 0.000 title description 6
- 239000000243 solution Substances 0.000 claims abstract description 244
- 108010055297 Sterol Esterase Proteins 0.000 claims abstract description 117
- 102000000019 Sterol Esterase Human genes 0.000 claims abstract description 117
- 102000004190 Enzymes Human genes 0.000 claims abstract description 106
- 108090000790 Enzymes Proteins 0.000 claims abstract description 106
- 239000007853 buffer solution Substances 0.000 claims abstract description 103
- 108010089254 Cholesterol oxidase Proteins 0.000 claims abstract description 98
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 claims abstract description 92
- 102000003992 Peroxidases Human genes 0.000 claims abstract description 51
- 108040007629 peroxidase activity proteins Proteins 0.000 claims abstract description 51
- 238000003556 assay Methods 0.000 claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000008620 Cholesterol Assay Methods 0.000 claims abstract description 25
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 claims abstract description 23
- BHQCQFFYRZLCQQ-UHFFFAOYSA-N (3alpha,5alpha,7alpha,12alpha)-3,7,12-trihydroxy-cholan-24-oic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 BHQCQFFYRZLCQQ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000004380 Cholic acid Substances 0.000 claims abstract description 20
- 229960002471 cholic acid Drugs 0.000 claims abstract description 20
- 235000019416 cholic acid Nutrition 0.000 claims abstract description 20
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 claims abstract description 20
- 150000003839 salts Chemical class 0.000 claims abstract description 16
- 239000003085 diluting agent Substances 0.000 claims abstract description 15
- 150000002894 organic compounds Chemical class 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims description 85
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 57
- 239000012141 concentrate Substances 0.000 claims description 44
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 38
- NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 claims description 31
- 239000004094 surface-active agent Substances 0.000 claims description 27
- 150000001875 compounds Chemical class 0.000 claims description 25
- 241000187654 Nocardia Species 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 244000005700 microbiome Species 0.000 claims description 17
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 15
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 15
- 241000589540 Pseudomonas fluorescens Species 0.000 claims description 13
- 230000036284 oxygen consumption Effects 0.000 claims description 13
- 108090000371 Esterases Proteins 0.000 claims description 12
- 229910052751 metal Inorganic materials 0.000 claims description 11
- 239000002184 metal Substances 0.000 claims description 11
- 150000001840 cholesterol esters Chemical class 0.000 claims description 9
- 240000003291 Armoracia rusticana Species 0.000 claims description 8
- 235000011330 Armoracia rusticana Nutrition 0.000 claims description 8
- 241000186311 Brevibacterium sterolicum Species 0.000 claims description 8
- 102000004316 Oxidoreductases Human genes 0.000 claims description 8
- 108090000854 Oxidoreductases Proteins 0.000 claims description 8
- 241000589774 Pseudomonas sp. Species 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 7
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical group [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 7
- 239000000872 buffer Substances 0.000 claims description 6
- 239000012062 aqueous buffer Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000007257 deesterification reaction Methods 0.000 claims description 3
- 239000012266 salt solution Substances 0.000 claims description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims 2
- 239000000600 sorbitol Substances 0.000 claims 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 1
- 239000008366 buffered solution Substances 0.000 abstract description 3
- 229940107161 cholesterol Drugs 0.000 description 107
- 229940088598 enzyme Drugs 0.000 description 97
- 239000003153 chemical reaction reagent Substances 0.000 description 70
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 38
- 235000008504 concentrate Nutrition 0.000 description 38
- 229940072417 peroxidase Drugs 0.000 description 38
- 230000000694 effects Effects 0.000 description 28
- 239000000203 mixture Substances 0.000 description 20
- 238000006243 chemical reaction Methods 0.000 description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 15
- 239000001301 oxygen Substances 0.000 description 15
- 229910052760 oxygen Inorganic materials 0.000 description 15
- 238000002835 absorbance Methods 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 13
- 241001465754 Metazoa Species 0.000 description 11
- 230000002255 enzymatic effect Effects 0.000 description 11
- 230000000813 microbial effect Effects 0.000 description 11
- 239000007788 liquid Substances 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- 238000002798 spectrophotometry method Methods 0.000 description 9
- 150000002500 ions Chemical class 0.000 description 8
- 150000002978 peroxides Chemical class 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000001008 quinone-imine dye Substances 0.000 description 6
- 238000005057 refrigeration Methods 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 238000004806 packaging method and process Methods 0.000 description 5
- 238000003908 quality control method Methods 0.000 description 5
- 159000000000 sodium salts Chemical class 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- NYOXRYYXRWJDKP-GYKMGIIDSA-N cholest-4-en-3-one Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 NYOXRYYXRWJDKP-GYKMGIIDSA-N 0.000 description 4
- NYOXRYYXRWJDKP-UHFFFAOYSA-N cholestenone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 NYOXRYYXRWJDKP-UHFFFAOYSA-N 0.000 description 4
- 239000000306 component Substances 0.000 description 4
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 230000035484 reaction time Effects 0.000 description 4
- 239000011369 resultant mixture Substances 0.000 description 4
- 238000007127 saponification reaction Methods 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229940099352 cholate Drugs 0.000 description 3
- 150000002148 esters Chemical group 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 210000000496 pancreas Anatomy 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 238000007086 side reaction Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- -1 terol ester Chemical class 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- 241000182988 Assa Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000004895 Lipoproteins Human genes 0.000 description 2
- 108090001030 Lipoproteins Proteins 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 240000001987 Pyrus communis Species 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000000368 destabilizing effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 2
- IBDXZWQCLMSDKQ-FDXOKOSPSA-N i-cholesterol Chemical compound C([C@H]1[C@@H]2CC[C@@H]([C@]2(CC[C@@H]1[C@@]1(C)CC2)C)[C@H](C)CCCC(C)C)[C@@H](O)[C@@]31[C@H]2C3 IBDXZWQCLMSDKQ-FDXOKOSPSA-N 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000011177 media preparation Methods 0.000 description 2
- 238000005065 mining Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 235000015277 pork Nutrition 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
- ORLFVWPPBMVPNZ-UHFFFAOYSA-N 1-(6-methylheptyl)-4-[4-(6-methylheptyl)phenoxy]benzene Chemical compound C1=CC(CCCCCC(C)C)=CC=C1OC1=CC=C(CCCCCC(C)C)C=C1 ORLFVWPPBMVPNZ-UHFFFAOYSA-N 0.000 description 1
- 241001182632 Akko Species 0.000 description 1
- 102000004867 Hydro-Lyases Human genes 0.000 description 1
- 108090001042 Hydro-Lyases Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 101100400378 Mus musculus Marveld2 gene Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 description 1
- 240000004760 Pimpinella anisum Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000708948 Solva Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 125000002877 alkyl aryl group Chemical group 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 239000012490 blank solution Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000011328 necessary treatment Methods 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- PLIKAWJENQZMHA-UHFFFAOYSA-N p-hydroxyphenylamine Natural products NC1=CC=C(O)C=C1 PLIKAWJENQZMHA-UHFFFAOYSA-N 0.000 description 1
- 210000001819 pancreatic juice Anatomy 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 235000011118 potassium hydroxide Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000002603 single-photon emission computed tomography Methods 0.000 description 1
- 238000007614 solvation Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/50—Phenols; Naphthols; Catechols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/90—Developer
- C12Q2326/96—4-Amino-antipyrine
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6891179A | 1979-08-23 | 1979-08-23 | |
| US068,911 | 1979-08-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1154366A true CA1154366A (en) | 1983-09-27 |
Family
ID=22085503
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000357900A Expired CA1154366A (en) | 1979-08-23 | 1980-08-08 | Enzymatic method and stabilized solutions for determining total cholesterol in human serum |
Country Status (5)
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3165392D1 (en) * | 1980-07-10 | 1984-09-13 | Ivan Endre Modrovich | Stabilized enzymatic solutions and method for determining total cholesterol in human serum |
| DE3125667C2 (de) * | 1981-06-30 | 1986-01-23 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Mittel zum Nachweis von Wasserstoffperoxid |
| DE3261832D1 (en) * | 1981-07-30 | 1985-02-21 | Hoffmann La Roche | Method for the stabilisation of peroxidase in a liquid medium, and the stabilised medium so obtained |
| DE3208253A1 (de) * | 1982-03-08 | 1983-09-15 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur spezifischen bestimmung des cholesterins der ldl-fraktion im serum |
| US5047327A (en) * | 1982-04-02 | 1991-09-10 | Ivan E. Modrovich | Time-stable liquid cholesterol assay compositions |
| ATE25464T1 (de) * | 1982-04-02 | 1987-02-15 | Ivan Endre Modrovich | Zeitstabile fluessige zusammensetzung fuer den cholesterinnachweis. |
| IT1177513B (it) * | 1984-01-27 | 1987-08-26 | Menarini Sas | Reattivo liquido pronto per l'uso per la determinazione del contenuto di glucosio nel sangue |
| GB2154735B (en) * | 1984-01-27 | 1987-07-15 | Menarini Sas | Reagent for determining blood glucose content |
| IL74177A (en) * | 1984-02-03 | 1989-09-10 | Abbott Lab | Stabilized enzyme conjugate composition containing a calcium salt and a polyethylene glycol |
| US4680259A (en) * | 1984-09-26 | 1987-07-14 | Eastman Kodak Company | Analytical element and method for colorimetric determination of total cholesterol |
| DE3511327A1 (de) * | 1985-03-28 | 1986-10-02 | Boehringer Mannheim Gmbh, 6800 Mannheim | Stabilisierung der aktivitaet von peroxidase in loesung |
| JPS62296A (ja) * | 1985-06-26 | 1987-01-06 | Kyowa Medetsukusu Kk | 過酸化水素の定量方法 |
| EP0259521A1 (en) * | 1986-09-10 | 1988-03-16 | Akzo N.V. | Test reagent for amylase determination |
| US5188801A (en) * | 1988-06-07 | 1993-02-23 | Cortek S.P.A. | Device for the treatment of blood |
| CN109971735B (zh) * | 2019-04-03 | 2021-09-28 | 江南大学 | 一种胆固醇酯酶的制备方法 |
| EP3722418A1 (en) * | 2019-04-08 | 2020-10-14 | AB Enzymes Oy | Solution stable enzyme composition |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2047147A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1969-05-06 | 1971-03-12 | Kyowa Hakko Kogyo Kk | |
| US3776900A (en) * | 1971-04-26 | 1973-12-04 | Searle & Co | Stabilization of reduced coenzymes |
| US4186251A (en) * | 1973-03-01 | 1980-01-29 | Miles Laboratories, Inc. | Composition and method for determination of cholesterol |
| DE2315501C3 (de) * | 1973-03-28 | 1980-02-21 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur Bestimmung von Cholesterin |
| GB1479994A (en) * | 1974-03-04 | 1977-07-13 | Abbott Lab | Single reagent for the enzymatic determination of cholesterol and method therefor |
| JPS50150496A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1974-05-22 | 1975-12-02 | ||
| US4153511A (en) * | 1976-03-17 | 1979-05-08 | Modrovich Ivan Endre | Stabilized liquid coenzyme compositions |
| US4121905A (en) * | 1977-08-22 | 1978-10-24 | Jonas Maurukas | Process for preparing biological compositions for use as reference controls in diagnostic analyses |
| JPS57125796A (en) * | 1981-01-29 | 1982-08-05 | Mitsubishi Electric Corp | Fixed position stopping device in continuous pattern sewing machine |
-
1980
- 1980-08-01 EP EP80104568A patent/EP0024578B1/en not_active Expired
- 1980-08-01 DE DE8080104568T patent/DE3065827D1/de not_active Expired
- 1980-08-01 AT AT80104568T patent/ATE5539T1/de not_active IP Right Cessation
- 1980-08-08 CA CA000357900A patent/CA1154366A/en not_active Expired
- 1980-08-22 JP JP11486180A patent/JPS5635999A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DE3065827D1 (en) | 1984-01-12 |
| JPS5635999A (en) | 1981-04-08 |
| EP0024578A1 (en) | 1981-03-11 |
| ATE5539T1 (de) | 1983-12-15 |
| EP0024578B1 (en) | 1983-12-07 |
| JPS6225040B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1987-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4378429A (en) | Enzymatic method and stabilized solutions for determining total cholesterol in human serum | |
| CA1154366A (en) | Enzymatic method and stabilized solutions for determining total cholesterol in human serum | |
| Shimizu et al. | Enzymatic microdetermination of serum free fatty acids | |
| Akimoto et al. | Luminol chemiluminescence reaction catalyzed by a microbial peroxidase | |
| EP0080304B1 (en) | Stabilization of oxidase | |
| Allain et al. | Enzymatic determination of total serum cholesterol | |
| US4517287A (en) | Method and reagent for the enzymatic determination of enzyme substrates | |
| Noma et al. | Polarographic method for rapid microdetermination of cholesterol with cholesterol esterase and cholesterol oxidase. | |
| US4212938A (en) | Reagent and method for the determination of cholesterol | |
| US3721607A (en) | Reagent composition and process for the determination of glucose | |
| US5278044A (en) | Stable aqueous NADH reagent and kit | |
| Artiss et al. | Bilirubin interference in a peroxidase-coupled procedure for creatinine eliminated by bilirubin oxidase. | |
| US3862012A (en) | Quantitative determination of uric acid | |
| JP2854995B2 (ja) | 尿酸測定用試薬組成物 | |
| US4409326A (en) | Stabilized enzymatic solutions and method for determining total cholesterol in human serum | |
| EP0044432B1 (en) | Stabilized enzymatic solutions and method for determining total cholesterol in human serum | |
| US5122454A (en) | Assay method for lecithin-cholesterol acyltransferase | |
| EP0121254A2 (en) | Process for determining substrate or enzymatic activity | |
| JP4639287B2 (ja) | 酵素的測定試薬の安定化方法 | |
| US5266472A (en) | Stabilization of the enzyme urate oxidase in liquid form | |
| US5221615A (en) | Liquid stable triglyceride reagent system | |
| US5474908A (en) | Method of enzymatically measuring hydrogen peroxide and reagent therefor | |
| EP0636885B1 (en) | Method for determining the fructosamine content | |
| US4206280A (en) | Determination of acid phosphatase | |
| Taniguchi et al. | Determination of cholesterol with a laboratory-built chemiluminescence system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |