CA1081589A - Composition and method for the quantitative determination of phospholipids - Google Patents

Composition and method for the quantitative determination of phospholipids

Info

Publication number
CA1081589A
CA1081589A CA284,986A CA284986A CA1081589A CA 1081589 A CA1081589 A CA 1081589A CA 284986 A CA284986 A CA 284986A CA 1081589 A CA1081589 A CA 1081589A
Authority
CA
Canada
Prior art keywords
choline
reaction
phospholipase
lecithin
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA284,986A
Other languages
English (en)
French (fr)
Inventor
Hiroaki Hayashi
Katsuyuki Watanabe
Toshio Tatano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Application granted granted Critical
Publication of CA1081589A publication Critical patent/CA1081589A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C12Q2326/92—Nitro blue tetrazolium chloride, i.e. NBT
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/906—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/04—Phospholipids, i.e. phosphoglycerides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA284,986A 1976-08-19 1977-08-18 Composition and method for the quantitative determination of phospholipids Expired CA1081589A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP98233/76 1976-08-19
JP9823376A JPS5324888A (en) 1976-08-19 1976-08-19 Composition for quantitating fats containing phosphorus

Publications (1)

Publication Number Publication Date
CA1081589A true CA1081589A (en) 1980-07-15

Family

ID=14214230

Family Applications (1)

Application Number Title Priority Date Filing Date
CA284,986A Expired CA1081589A (en) 1976-08-19 1977-08-18 Composition and method for the quantitative determination of phospholipids

Country Status (4)

Country Link
JP (1) JPS5324888A (enFirst)
CA (1) CA1081589A (enFirst)
DE (1) DE2737513B2 (enFirst)
FR (1) FR2362394A1 (enFirst)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5937737B2 (ja) * 1978-05-25 1984-09-11 新日本製鐵株式会社 熱間鍛造用鋼材
IT1157281B (it) * 1981-06-25 1987-02-11 Toyo Jozo Kk Metodo di saggio per un componente relativo ai lipidi, composizione per il saggio e procedimento per la produzione dell'enzima utilizzato allo scopo
DE3128480A1 (de) * 1981-07-18 1983-01-27 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur bestimmung von lecithin in fruchtwasser sowie reagens zur durchfuehrung dieses verfahrens
JPS58107199A (ja) * 1981-12-16 1983-06-25 Toyo Jozo Co Ltd ホスフアチジルグリセロ−ルの定量方法
DE4409987C1 (de) * 1994-03-23 1995-07-06 Wolfgang Dr Bernhard Hochdruck-Flüssigkeitschromatographieverfahren zur Trennung der Hauptphospholipidklassen
JP4527925B2 (ja) * 2000-02-08 2010-08-18 シスメックス株式会社 腎障害の検査方法

Also Published As

Publication number Publication date
FR2362394A1 (fr) 1978-03-17
DE2737513A1 (de) 1978-02-23
DE2737513B2 (de) 1980-02-14
JPS5324888A (en) 1978-03-08
FR2362394B1 (enFirst) 1981-08-28

Similar Documents

Publication Publication Date Title
KR920009425B1 (ko) 유체중 이온의 측정
US4350762A (en) Aminopyrine improved Trinder's reagent and dosing process for hydrogen peroxide from enzymatic oxidation of metabolic substrata with the same
US4245041A (en) Triglycerides assay and reagents therefor
US5384247A (en) Determination of sodium ions in fluids
Richmond Analytical reviews in clinical biochemistry: the quantitative analysis of cholesterol
Shephard et al. Falsely low estimation of triglycerides in lipemic plasma by the enzymatic triglyceride method with modified Trinder's chromogen
EP0116307B1 (en) Composition, analytical element and method for the quantification of creatine kinase
Wimmer et al. A kinetic colorimetric procedure for quantifying magnesium in serum.
CA1081589A (en) Composition and method for the quantitative determination of phospholipids
US4141792A (en) Composition and method for the quantitative determination of phospholipids
Smith et al. Automated measurement of total cholesterol and triglycerides, in" tandem," on the discrete sample analyzer, Gilford System 3500.
Wevers et al. A bioluminescent assay for enolase (EC 4.2. 1.11) activity in human serum and cerebrospinal fluid
US7374897B2 (en) Enzyme cycling based assays for alpha-methylacyl-CoA racemase
US4816394A (en) Quantitative analysis of 3α-hydroxysteroid and reagent useful therefor
Hearne et al. Assessment of colorimetric enzymatic determination of triglyceride, by manual and centrifugal analyzer techniques, and comparison with a CDC standardized method
US5919644A (en) Method for assaying sulfate-conjugated bile acid and therefore
EP0245528B1 (en) Quantitative analysis of 3 alpha-hydroxysteroid and reagent useful therefor
CA1201047A (en) Method for quantitative measurement of phosphatidyl glycerol
EP0159513A1 (en) Enzymatic inorganic phosphate assay
IE47123B1 (en) Analytical device
JPH1099098A (ja) トレハロース分析用試薬
Yuan et al. Spectrophotometric and fluorimetric enzymatic determination of serum creatine kinase mb isoenzyme by using immuno-inhibition
Dörner Quantitative determination of lactose, maltose, and sucrose in urine
GB2050601A (en) An Improved Triglycerides Assay and Reagents Therefor
Avigad et al. A coupled NAD+-peroxidase spectrophotometric assay for cholesterol.

Legal Events

Date Code Title Description
MKEX Expiry