CA1047918A - Method and apparatus for detection and purification of proteins and antibodies - Google Patents
Method and apparatus for detection and purification of proteins and antibodiesInfo
- Publication number
- CA1047918A CA1047918A CA204,262A CA204262A CA1047918A CA 1047918 A CA1047918 A CA 1047918A CA 204262 A CA204262 A CA 204262A CA 1047918 A CA1047918 A CA 1047918A
- Authority
- CA
- Canada
- Prior art keywords
- protein
- substrate
- metal
- layer
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims abstract description 45
- 102000004169 proteins and genes Human genes 0.000 title abstract description 198
- 108090000623 proteins and genes Proteins 0.000 title abstract description 198
- 238000001514 detection method Methods 0.000 title abstract description 18
- 238000000746 purification Methods 0.000 title abstract description 10
- 239000000758 substrate Substances 0.000 claims abstract description 129
- 239000000427 antigen Substances 0.000 claims abstract description 64
- 102000036639 antigens Human genes 0.000 claims abstract description 62
- 108091007433 antigens Proteins 0.000 claims abstract description 62
- 239000002052 molecular layer Substances 0.000 claims abstract description 11
- 229910052751 metal Inorganic materials 0.000 claims description 59
- 239000002184 metal Substances 0.000 claims description 59
- 229910052737 gold Inorganic materials 0.000 claims description 23
- 239000010931 gold Substances 0.000 claims description 23
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 15
- 229910052738 indium Inorganic materials 0.000 claims description 12
- APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 10
- 238000005259 measurement Methods 0.000 claims description 8
- 238000002405 diagnostic procedure Methods 0.000 claims description 7
- 238000002310 reflectometry Methods 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 238000000576 coating method Methods 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 3
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 3
- 239000011133 lead Substances 0.000 claims description 3
- 229910044991 metal oxide Inorganic materials 0.000 claims description 3
- 150000004706 metal oxides Chemical class 0.000 claims description 3
- 239000010445 mica Substances 0.000 claims description 3
- 229910052618 mica group Inorganic materials 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- 229910052709 silver Inorganic materials 0.000 claims description 3
- 239000004332 silver Substances 0.000 claims description 3
- 239000011135 tin Substances 0.000 claims description 3
- 229910052718 tin Inorganic materials 0.000 claims description 3
- 239000005350 fused silica glass Substances 0.000 claims description 2
- 238000009736 wetting Methods 0.000 claims description 2
- 238000000151 deposition Methods 0.000 claims 5
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims 2
- 239000012736 aqueous medium Substances 0.000 claims 2
- 239000010936 titanium Substances 0.000 claims 2
- 229910052719 titanium Inorganic materials 0.000 claims 2
- 239000012472 biological sample Substances 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
- 239000002253 acid Substances 0.000 abstract description 25
- 230000001900 immune effect Effects 0.000 abstract description 10
- 230000003287 optical effect Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 76
- 238000012360 testing method Methods 0.000 description 40
- 208000006454 hepatitis Diseases 0.000 description 27
- 231100000283 hepatitis Toxicity 0.000 description 27
- 210000002966 serum Anatomy 0.000 description 22
- 239000010408 film Substances 0.000 description 16
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 14
- 229940098773 bovine serum albumin Drugs 0.000 description 14
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 14
- 229910052753 mercury Inorganic materials 0.000 description 14
- 210000004369 blood Anatomy 0.000 description 13
- 239000008280 blood Substances 0.000 description 13
- 230000008105 immune reaction Effects 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 9
- 239000002245 particle Substances 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 108010058846 Ovalbumin Proteins 0.000 description 6
- 239000004793 Polystyrene Substances 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 230000000536 complexating effect Effects 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 229940092253 ovalbumin Drugs 0.000 description 6
- 229920002223 polystyrene Polymers 0.000 description 6
- 210000002700 urine Anatomy 0.000 description 6
- 229910000497 Amalgam Inorganic materials 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 230000004520 agglutination Effects 0.000 description 4
- 239000003990 capacitor Substances 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 238000001465 metallisation Methods 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- -1 fused sili~a Substances 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000009597 pregnancy test Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 241000518994 Conta Species 0.000 description 1
- 101100270435 Mus musculus Arhgef12 gene Proteins 0.000 description 1
- 241001163743 Perlodes Species 0.000 description 1
- XDXHAEQXIBQUEZ-UHFFFAOYSA-N Ropinirole hydrochloride Chemical compound Cl.CCCN(CCC)CCC1=CC=CC2=C1CC(=O)N2 XDXHAEQXIBQUEZ-UHFFFAOYSA-N 0.000 description 1
- 241000950638 Symphysodon discus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004523 agglutinating effect Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009582 blood typing Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- JJCFRYNCJDLXIK-UHFFFAOYSA-N cyproheptadine Chemical compound C1CN(C)CCC1=C1C2=CC=CC=C2C=CC2=CC=CC=C21 JJCFRYNCJDLXIK-UHFFFAOYSA-N 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000007888 film coating Substances 0.000 description 1
- 238000009501 film coating Methods 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- HOQADATXFBOEGG-UHFFFAOYSA-N isofenphos Chemical compound CCOP(=S)(NC(C)C)OC1=CC=CC=C1C(=O)OC(C)C HOQADATXFBOEGG-UHFFFAOYSA-N 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 229910052715 tantalum Inorganic materials 0.000 description 1
- GUVRBAGPIYLISA-UHFFFAOYSA-N tantalum atom Chemical compound [Ta] GUVRBAGPIYLISA-UHFFFAOYSA-N 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000001429 visible spectrum Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nanotechnology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Materials Engineering (AREA)
- Pharmacology & Pharmacy (AREA)
- Inorganic Chemistry (AREA)
- Composite Materials (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medical Informatics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Light Receiving Elements (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38411373A | 1973-07-30 | 1973-07-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1047918A true CA1047918A (en) | 1979-02-06 |
Family
ID=23516091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA204,262A Expired CA1047918A (en) | 1973-07-30 | 1974-07-08 | Method and apparatus for detection and purification of proteins and antibodies |
Country Status (7)
| Country | Link |
|---|---|
| JP (2) | JPS5944583B2 (enrdf_load_stackoverflow) |
| BR (1) | BR7406086D0 (enrdf_load_stackoverflow) |
| CA (1) | CA1047918A (enrdf_load_stackoverflow) |
| DE (2) | DE2433246C2 (enrdf_load_stackoverflow) |
| IT (1) | IT1212274B (enrdf_load_stackoverflow) |
| NL (1) | NL177939C (enrdf_load_stackoverflow) |
| SE (1) | SE464784B (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5783455A (en) * | 1994-10-15 | 1998-07-21 | Behring Diagnostics Gmbh | Regenerable solid phase for carrying out specific binding reactions |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5238789A (en) * | 1975-08-14 | 1977-03-25 | Sinai School Medicine | Method of identifying hematocyte type and adaptability |
| DE2638251A1 (de) * | 1975-08-27 | 1977-06-08 | Gen Electric | Verfahren und vorrichtung zum konzentrieren und reinigen von antigenen und antikoerpern |
| DE2638250C2 (de) * | 1975-08-27 | 1985-11-28 | General Electric Co., Schenectady, N.Y. | Diagnostisches Verfahren zum Bestimmen der Anwesenheit oder Abwesenheit eines spezifischen Proteins in einer biologischen Probe, sowie Vorrichtung zur Durchführung des Verfahrens |
| DE3176524D1 (en) * | 1981-06-22 | 1987-12-17 | Battelle Memorial Institute | A method for determining bioactive substances |
| DE3215484A1 (de) * | 1982-04-26 | 1983-11-03 | Sagax Instrument AB, 18302 Täby | Aus mehreren schichten bestehender schichtkoerper und verfahren zum nachweis und/oder messen der konzentration einer chemischen substanz, insbesondere biologischer herkunft |
| JPS6432150A (en) * | 1987-07-29 | 1989-02-02 | Teijin Ltd | Reflection color measuring instrument |
| JPH073388B2 (ja) * | 1987-08-10 | 1995-01-18 | 帝人株式会社 | 膜厚変化測定装置 |
| JPH0188778U (enrdf_load_stackoverflow) * | 1987-12-02 | 1989-06-12 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1025772A (en) * | 1972-06-26 | 1978-02-07 | Ivar Giaever | Method and apparatus for detection and purification of proteins and antibodies |
-
1974
- 1974-07-08 CA CA204,262A patent/CA1047918A/en not_active Expired
- 1974-07-11 DE DE19742433246 patent/DE2433246C2/de not_active Expired
- 1974-07-11 DE DE19742463435 patent/DE2463435C2/de not_active Expired
- 1974-07-24 BR BR608674A patent/BR7406086D0/pt unknown
- 1974-07-29 IT IT2566674A patent/IT1212274B/it active
- 1974-07-29 SE SE7409777A patent/SE464784B/xx not_active IP Right Cessation
- 1974-07-30 JP JP8665874A patent/JPS5944583B2/ja not_active Expired
- 1974-07-30 NL NL7410231A patent/NL177939C/xx not_active IP Right Cessation
-
1983
- 1983-10-28 JP JP20117483A patent/JPS59160763A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5783455A (en) * | 1994-10-15 | 1998-07-21 | Behring Diagnostics Gmbh | Regenerable solid phase for carrying out specific binding reactions |
Also Published As
| Publication number | Publication date |
|---|---|
| NL7410231A (nl) | 1975-02-03 |
| JPS59160763A (ja) | 1984-09-11 |
| SE7409777L (enrdf_load_stackoverflow) | 1975-01-31 |
| DE2463435C2 (de) | 1986-12-04 |
| BR7406086D0 (pt) | 1975-05-13 |
| DE2433246C2 (de) | 1986-04-17 |
| JPS5076226A (enrdf_load_stackoverflow) | 1975-06-21 |
| IT1212274B (it) | 1989-11-22 |
| DE2433246A1 (de) | 1975-02-13 |
| NL177939C (nl) | 1985-12-16 |
| NL177939B (nl) | 1985-07-16 |
| SE464784B (sv) | 1991-06-10 |
| JPS6130215B2 (enrdf_load_stackoverflow) | 1986-07-11 |
| JPS5944583B2 (ja) | 1984-10-30 |
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