BRPI1100418B1 - processo de modificação genética de bactérias acidófilas - Google Patents

processo de modificação genética de bactérias acidófilas

Info

Publication number
BRPI1100418B1
BRPI1100418B1 BRPI1100418A BRPI1100418A BRPI1100418B1 BR PI1100418 B1 BRPI1100418 B1 BR PI1100418B1 BR PI1100418 A BRPI1100418 A BR PI1100418A BR PI1100418 A BRPI1100418 A BR PI1100418A BR PI1100418 B1 BRPI1100418 B1 BR PI1100418B1
Authority
BR
Brazil
Prior art keywords
bacteria
vector
genetic modification
acidophilic bacteria
transforming
Prior art date
Application number
BRPI1100418A
Other languages
English (en)
Inventor
Paula Guarnieri Christ Ana
Alexandrino Fabiana
M M Ottoboni Laura
Garcia Oswaldo Jr
Original Assignee
Unicamp Univ Estadual De Campinas
Univ Estadual Paulista Julio De Mesquita Filho
Vale Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unicamp Univ Estadual De Campinas, Univ Estadual Paulista Julio De Mesquita Filho, Vale Sa filed Critical Unicamp Univ Estadual De Campinas
Priority to BRPI1100418A priority Critical patent/BRPI1100418B1/pt
Priority to JP2013553749A priority patent/JP2014505483A/ja
Priority to PCT/BR2012/000045 priority patent/WO2012116422A2/pt
Priority to CA2832932A priority patent/CA2832932A1/en
Priority to AU2012222880A priority patent/AU2012222880A1/en
Priority to RU2013142578/10A priority patent/RU2013142578A/ru
Priority to EP12726722.7A priority patent/EP2677033A2/en
Publication of BRPI1100418A2 publication Critical patent/BRPI1100418A2/pt
Publication of BRPI1100418B1 publication Critical patent/BRPI1100418B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

processo de modificação genética de bactérias acidófilas e construção de um vetor de transformação. a presente invenção descreve um processo de transformação de bactérias acidôfilas quimiolitotróficas através da técnica de eletroporação. o processo proposto é capaz de transformar uma linhagem de bactéria com um vetor de transformação denominado vetor paf que contém uma origem vegetativa de replicação capaz de conferir ao vetor a habilidade de se replicar no interior da bactéria, sem alterar suas funções fisiológicas naturais e, o uso das bactérias modificadas de acordo com a invenção em processos de biolixiviação de minérios sulfatados de cobre, ouro, urânio níquel, zinco, cobalto e outros.
BRPI1100418A 2011-02-18 2011-02-18 processo de modificação genética de bactérias acidófilas BRPI1100418B1 (pt)

Priority Applications (7)

Application Number Priority Date Filing Date Title
BRPI1100418A BRPI1100418B1 (pt) 2011-02-18 2011-02-18 processo de modificação genética de bactérias acidófilas
JP2013553749A JP2014505483A (ja) 2011-02-18 2012-02-17 好酸性細菌の遺伝子操作および形質転換ベクターの構築方法
PCT/BR2012/000045 WO2012116422A2 (pt) 2011-02-18 2012-02-17 Processo de modificação genética de bactérias acidófilas e construção de um vetor de transformação
CA2832932A CA2832932A1 (en) 2011-02-18 2012-02-17 Process for genetically engineering acidophilic bacteria and constructing a transformation vector
AU2012222880A AU2012222880A1 (en) 2011-02-18 2012-02-17 Method for genetically modifying acidophilic bacteria and constructing a transformation vector
RU2013142578/10A RU2013142578A (ru) 2011-02-18 2012-02-17 Способ генной инженерии ацидофильных бактерий и получения вектора для трансформации
EP12726722.7A EP2677033A2 (en) 2011-02-18 2012-02-17 Method for genetically modifying acidophilic bacteria and constructing a transformation vector

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI1100418A BRPI1100418B1 (pt) 2011-02-18 2011-02-18 processo de modificação genética de bactérias acidófilas

Publications (2)

Publication Number Publication Date
BRPI1100418A2 BRPI1100418A2 (pt) 2013-05-21
BRPI1100418B1 true BRPI1100418B1 (pt) 2021-03-02

Family

ID=46245750

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1100418A BRPI1100418B1 (pt) 2011-02-18 2011-02-18 processo de modificação genética de bactérias acidófilas

Country Status (7)

Country Link
EP (1) EP2677033A2 (pt)
JP (1) JP2014505483A (pt)
AU (1) AU2012222880A1 (pt)
BR (1) BRPI1100418B1 (pt)
CA (1) CA2832932A1 (pt)
RU (1) RU2013142578A (pt)
WO (1) WO2012116422A2 (pt)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138267B1 (en) * 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
BRPI0806029A2 (pt) * 2007-07-19 2009-11-10 Biosigma Sa plasmìdeos para transformação de bactérias do gênero aciditiobacillus spp. e método de transformação

Also Published As

Publication number Publication date
WO2012116422A3 (pt) 2013-03-28
RU2013142578A (ru) 2015-03-27
AU2012222880A2 (en) 2013-11-07
AU2012222880A1 (en) 2013-10-03
BRPI1100418A2 (pt) 2013-05-21
JP2014505483A (ja) 2014-03-06
WO2012116422A2 (pt) 2012-09-07
EP2677033A2 (en) 2013-12-25
CA2832932A1 (en) 2012-09-07

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Legal Events

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B15L Others concerning applications: renumbering

Free format text: RENUMERADO O PEDIDO DE PI1100418-5 PARA PP1100418-5.

B03A Publication of an application: publication of a patent application or of a certificate of addition of invention
B08E Application fees: requirement for complementation of annuity fee

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B08G Application fees: restoration
B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B25M Limitations in changing the proprietorship

Owner name: VALE S.A. (BR/RJ) ; UNIVERSIDADE ESTADUAL PAULISTA JULIO DE MESQUITA FILHO (BR/SP) ; UNICAMP - UNIVERSIDADE ESTADUAL DE CAMPINAS (BR/SP)

Free format text: ANOTACAO DE LIMITACAO OU ONUSREFERENCIA: PROCESSO SINPI/SEI 52402.005510/2018-81 FICA ANOTADA A INDISPONIBILIDADE DO REFERIDO PEDIDO DE PATENTE, DE ACORDO COM O ART. 59,II, DA LPI, CONFORME DETERMINADO PELO MM. JUIZ FEDERAL DA 27A VARA FEDERAL. CONFORME A MESMADETERMINACAO NAO SERA POSSIVEL A TRANSFERENCIA DESTE, SALVO A AUTORIZACAO DESSE JUIZO.

Owner name: VALE S.A. (BR/RJ) ; UNIVERSIDADE ESTADUAL PAULISTA

Free format text: ANOTACAO DE LIMITACAO OU ONUSREFERENCIA: PROCESSO SINPI/SEI 52402.005510/2018-81 FICA ANOTADA A INDISPONIBILIDADE DO REFERIDO PEDIDO DE PATENTE, DE ACORDO COM O ART. 59,II, DA LPI, CONFORME DETERMINADO PELO MM. JUIZ FEDERAL DA 27A VARA FEDERAL. CONFORME A MESMADETERMINACAO NAO SERA POSSIVEL A TRANSFERENCIA DESTE, SALVO A AUTORIZACAO DESSE JUIZO.

B25M Limitations in changing the proprietorship

Owner name: VALE S.A. (BR/RJ) ; UNIVERSIDADE ESTADUAL PAULISTA

Free format text: ANOTACAO DE LIMITACAO OU ONUSREFERENCIA: PROCESSO SINPI/SEI 52402.005792/2018-17 CONFORME DETERMINADO PELA MMA. JUIZA FEDERAL MARIA ADNA FAGUNDES VELOSO DA 15A VARA NA TITULARIDADE DA 27A VARA FEDERAL, FICA ANOTADO CANCELAMENTO PROVISORIO DA INDISPONIBILIDADE PUBLICADA NA RPI 2491 DE 02/10/2018, DE ACORDO COM O ART. 59, II, DA LPI.

B06V Preliminary requirement: requests without searches performed by other patent offices: suspension of the patent application procedure
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Free format text: ANULADA A PUBLICACAO CODIGO 6.22 NA RPI NO 2548 DE 05/11/2019 POR TER SIDO EFETUADA COM INCORRECOES.

B06V Preliminary requirement: requests without searches performed by other patent offices: suspension of the patent application procedure
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