BRPI0418992A - processo para a purificação de hormÈnio do crescimento humano - Google Patents
processo para a purificação de hormÈnio do crescimento humanoInfo
- Publication number
- BRPI0418992A BRPI0418992A BRPI0418992-2A BRPI0418992A BRPI0418992A BR PI0418992 A BRPI0418992 A BR PI0418992A BR PI0418992 A BRPI0418992 A BR PI0418992A BR PI0418992 A BRPI0418992 A BR PI0418992A
- Authority
- BR
- Brazil
- Prior art keywords
- detergent
- growth hormone
- human growth
- elution buffer
- buffer
- Prior art date
Links
- 102000002265 Human Growth Hormone Human genes 0.000 title abstract 2
- 108010000521 Human Growth Hormone Proteins 0.000 title abstract 2
- 239000000854 Human Growth Hormone Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title 1
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 239000003599 detergent Substances 0.000 abstract 2
- 239000012149 elution buffer Substances 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 239000000872 buffer Substances 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 230000002452 interceptive effect Effects 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Endocrinology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PROCESSO PARA A PURIFICAçãO DE HORMÈNIO DO CRESCIMENTO HUMANO. A presente invenção refere-se a um processo comercial para a purificação de hormónio do crescimento humano obtido por uma técnica de DNA recombinante usando cromatografia interativa hidrofóbica, eficiente para separar moléculas encurtadas de hormónio do crescimento humano, presentes como uma impureza que surge devido a posições de corte presentes na megamolécula de hormónio do crescimento humano, da molécula de hormónio do crescimento humano-alvo, usando uma coluna fenila, uma mistura de tampões aquosos e solventes orgânicos como eluente, seguido por dessalinização, liofilização, e a invenção também refere-se ao processo em que a eluição de moléculas encurtadas de hormónio do crescimento humano precede a eluição do hormónio do crescimento humano-alvo, para se obter hGH de pureza > 99,5%.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IN2004/000346 WO2006051554A1 (en) | 2004-11-09 | 2004-11-09 | A novel process for purification of human growth harmone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0418992A true BRPI0418992A (pt) | 2007-12-11 |
Family
ID=34964528
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0418992-2A BRPI0418992A (pt) | 2004-11-09 | 2004-11-09 | processo para a purificação de hormÈnio do crescimento humano |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20080167452A1 (pt) |
| EP (1) | EP1809664B1 (pt) |
| CN (1) | CN101061137A (pt) |
| AT (1) | ATE395360T1 (pt) |
| AU (1) | AU2004324756A1 (pt) |
| BR (1) | BRPI0418992A (pt) |
| CA (1) | CA2565209A1 (pt) |
| DE (1) | DE602004013847D1 (pt) |
| EA (1) | EA200602097A1 (pt) |
| WO (1) | WO2006051554A1 (pt) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006073846A2 (en) * | 2004-12-22 | 2006-07-13 | Ambrx, Inc. | Methods for expression and purification of recombinant human growth hormone |
| WO2010096394A2 (en) | 2009-02-17 | 2010-08-26 | Redwood Biosciences, Inc. | Aldehyde-tagged protein-based drug carriers and methods of use |
| US9488625B2 (en) | 2010-12-15 | 2016-11-08 | Baxalta GmbH | Purification of factor VIII using a conductivity gradient |
| KR20140016262A (ko) | 2011-01-14 | 2014-02-07 | 레드우드 바이오사이언스 인코포레이티드 | 알데하이드-태깅된 면역글로불린 폴리펩타이드 및 이의 사용 방법 |
| US8679140B2 (en) | 2012-05-30 | 2014-03-25 | Covidien Lp | Surgical clamping device with ratcheting grip lock |
| US11208632B2 (en) | 2016-04-26 | 2021-12-28 | R.P. Scherer Technologies, Llc | Antibody conjugates and methods of making and using the same |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5630925A (en) * | 1979-08-21 | 1981-03-28 | Sumitomo Chem Co Ltd | Purification of human growth hormone |
| DK309184D0 (da) * | 1984-06-25 | 1984-06-25 | Nordisk Insulinlab | Fremgangsmaade til isolering af insulin eller insulinlignende materiale fra en fermenteringsvaeske |
| IT1223577B (it) * | 1987-12-22 | 1990-09-19 | Eniricerche Spa | Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura |
| DD285113A5 (de) * | 1989-06-21 | 1990-12-05 | Akad Wissenschaften Ddr | Verfahren zur gewinnung von reinem menschlichen wachstumshormon (hgh) |
| IL118201A (en) * | 1995-05-11 | 2004-12-15 | Roche Diagnostics Gmbh | Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof |
| US6451347B1 (en) * | 1999-03-01 | 2002-09-17 | Alkermes Controlled Therapeutics, Inc. | Method for purifying human growth hormone |
-
2004
- 2004-11-09 AU AU2004324756A patent/AU2004324756A1/en not_active Abandoned
- 2004-11-09 EA EA200602097A patent/EA200602097A1/ru unknown
- 2004-11-09 CA CA002565209A patent/CA2565209A1/en not_active Abandoned
- 2004-11-09 BR BRPI0418992-2A patent/BRPI0418992A/pt not_active IP Right Cessation
- 2004-11-09 CN CNA2004800435652A patent/CN101061137A/zh active Pending
- 2004-11-09 US US11/568,235 patent/US20080167452A1/en not_active Abandoned
- 2004-11-09 DE DE602004013847T patent/DE602004013847D1/de not_active Expired - Fee Related
- 2004-11-09 WO PCT/IN2004/000346 patent/WO2006051554A1/en active IP Right Grant
- 2004-11-09 EP EP04821383A patent/EP1809664B1/en active Active
- 2004-11-09 AT AT04821383T patent/ATE395360T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2004324756A1 (en) | 2006-05-18 |
| CN101061137A (zh) | 2007-10-24 |
| EP1809664A1 (en) | 2007-07-25 |
| EP1809664B1 (en) | 2008-05-14 |
| US20080167452A1 (en) | 2008-07-10 |
| DE602004013847D1 (de) | 2008-06-26 |
| ATE395360T1 (de) | 2008-05-15 |
| EA200602097A1 (ru) | 2007-04-27 |
| WO2006051554A1 (en) | 2006-05-18 |
| CA2565209A1 (en) | 2006-05-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 4A,5A,6A E 7A ANUIDADE(S) |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2129 DE 25/10/2011. |