BR112023027276A2 - Complexo, conjunto, kit, método para substituição in vitro de uma região alvo, e, método in vitro ou ex vivo para editar o genoma de uma célula - Google Patents

Complexo, conjunto, kit, método para substituição in vitro de uma região alvo, e, método in vitro ou ex vivo para editar o genoma de uma célula

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Publication number
BR112023027276A2
BR112023027276A2 BR112023027276A BR112023027276A BR112023027276A2 BR 112023027276 A2 BR112023027276 A2 BR 112023027276A2 BR 112023027276 A BR112023027276 A BR 112023027276A BR 112023027276 A BR112023027276 A BR 112023027276A BR 112023027276 A2 BR112023027276 A2 BR 112023027276A2
Authority
BR
Brazil
Prior art keywords
vitro
complex
editing
genome
kit
Prior art date
Application number
BR112023027276A
Other languages
English (en)
Inventor
François Cherbonneau
Original Assignee
Quidditas Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quidditas Sa filed Critical Quidditas Sa
Publication of BR112023027276A2 publication Critical patent/BR112023027276A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

complexo, conjunto, kit, método para substituição invitro de uma região alvo, e, método in vitro ou ex vivo para editar o genoma de uma célula. a invenção se refere a um complexo molecular que contém: - uma primeira molécula de ácido nucleico de fita simples que compreende pelo menos dois meios sítios de ligação de uma transposase, e - uma segunda molécula de ácido nucleico de fita simples que compreende pelo menos um meio sítio de ligação de uma transposase. o complexo é de modo que o primeiro e o segundo ácidos nucleicos de fita simples são emparelhados de acordo com a complementaridade de base definida por watson e crick de modo a definir dois sítios de ligação de fita dupla da dita transposase. a invenção se refere ainda ao uso do dito complexo, em particular para edição de dna.
BR112023027276A 2021-06-25 2022-06-24 Complexo, conjunto, kit, método para substituição in vitro de uma região alvo, e, método in vitro ou ex vivo para editar o genoma de uma célula BR112023027276A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2106868A FR3124522A1 (fr) 2021-06-25 2021-06-25 Composition et méthode permettant l’édition génomique
PCT/EP2022/067351 WO2022269037A1 (fr) 2021-06-25 2022-06-24 Composition et méthode permettant l'édition génomique

Publications (1)

Publication Number Publication Date
BR112023027276A2 true BR112023027276A2 (pt) 2024-03-12

Family

ID=79269675

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023027276A BR112023027276A2 (pt) 2021-06-25 2022-06-24 Complexo, conjunto, kit, método para substituição in vitro de uma região alvo, e, método in vitro ou ex vivo para editar o genoma de uma célula

Country Status (9)

Country Link
EP (1) EP4341397A1 (pt)
JP (1) JP2024522821A (pt)
KR (1) KR20240025636A (pt)
CN (1) CN117716029A (pt)
AU (1) AU2022296859A1 (pt)
BR (1) BR112023027276A2 (pt)
CA (1) CA3221597A1 (pt)
FR (1) FR3124522A1 (pt)
WO (1) WO2022269037A1 (pt)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140186843A1 (en) 2012-12-12 2014-07-03 Massachusetts Institute Of Technology Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
WO2019046636A1 (en) * 2017-08-30 2019-03-07 The Broad Institute, Inc. CRISPR BASED EDITION OF HDR TYPE WITH DOUBLE SELECTION
AU2020355025A1 (en) * 2019-09-23 2022-04-07 Regents Of The University Of Minnesota Genetically-edited immune cells and methods of therapy

Also Published As

Publication number Publication date
FR3124522A1 (fr) 2022-12-30
EP4341397A1 (fr) 2024-03-27
WO2022269037A1 (fr) 2022-12-29
KR20240025636A (ko) 2024-02-27
CA3221597A1 (fr) 2022-12-29
CN117716029A (zh) 2024-03-15
AU2022296859A1 (en) 2024-01-18
JP2024522821A (ja) 2024-06-21

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