BR112023026176A2 - Vetores de expressão, vetores livres de sequência bacteriana e métodos de fabricação e uso dos mesmos - Google Patents
Vetores de expressão, vetores livres de sequência bacteriana e métodos de fabricação e uso dos mesmosInfo
- Publication number
- BR112023026176A2 BR112023026176A2 BR112023026176A BR112023026176A BR112023026176A2 BR 112023026176 A2 BR112023026176 A2 BR 112023026176A2 BR 112023026176 A BR112023026176 A BR 112023026176A BR 112023026176 A BR112023026176 A BR 112023026176A BR 112023026176 A2 BR112023026176 A2 BR 112023026176A2
- Authority
- BR
- Brazil
- Prior art keywords
- vectors
- methods
- free
- bacterial sequence
- manufacture
- Prior art date
Links
- 239000013598 vector Substances 0.000 title abstract 7
- 230000001580 bacterial effect Effects 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- 239000013604 expression vector Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 108091028649 Multicopy single-stranded DNA Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16141—Use of virus, viral particle or viral elements as a vector
- C12N2710/16143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16141—Use of virus, viral particle or viral elements as a vector
- C12N2710/16145—Special targeting system for viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Virology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
vetores de expressão, vetores livres de sequência bacteriana e métodos de fabricação e uso dos mesmos. a presente invenção refere-se a vetores de expressão, vetores livres de sequência bacteriana, tal como dna ministring (msdna), e métodos de fabricação dos vetores livres de sequência bacteriana, incluindo com sistemas de produção de vetor. a presente invenção também provê composições compreendendo os vetores e usos dos vetores e composições.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202163211343P | 2021-06-16 | 2021-06-16 | |
US202263306015P | 2022-02-02 | 2022-02-02 | |
US202263331638P | 2022-04-15 | 2022-04-15 | |
PCT/IB2022/055620 WO2022264095A1 (en) | 2021-06-16 | 2022-06-16 | Expression vectors, bacterial sequence-free vectors, and methods of making and using the same |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112023026176A2 true BR112023026176A2 (pt) | 2024-03-05 |
Family
ID=84527300
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112023026176A BR112023026176A2 (pt) | 2021-06-16 | 2022-06-16 | Vetores de expressão, vetores livres de sequência bacteriana e métodos de fabricação e uso dos mesmos |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP4355883A1 (pt) |
JP (1) | JP2024523343A (pt) |
KR (1) | KR20240021906A (pt) |
AU (1) | AU2022292197A1 (pt) |
BR (1) | BR112023026176A2 (pt) |
CA (1) | CA3222578A1 (pt) |
WO (1) | WO2022264095A1 (pt) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024058155A1 (ja) * | 2022-09-12 | 2024-03-21 | 株式会社カネカ | 二本鎖環状dnaベクター、直鎖状共有結合閉鎖dnaの作製方法、並びにプロテロメラーゼ及びエンドヌクレアーゼを含む融合ポリペプチド |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9862954B2 (en) * | 2012-11-22 | 2018-01-09 | Mediphage Bioceuticals, Inc. | DNA vector production system |
-
2022
- 2022-06-16 BR BR112023026176A patent/BR112023026176A2/pt unknown
- 2022-06-16 EP EP22824423.2A patent/EP4355883A1/en active Pending
- 2022-06-16 AU AU2022292197A patent/AU2022292197A1/en active Pending
- 2022-06-16 CA CA3222578A patent/CA3222578A1/en active Pending
- 2022-06-16 WO PCT/IB2022/055620 patent/WO2022264095A1/en active Application Filing
- 2022-06-16 KR KR1020247001300A patent/KR20240021906A/ko unknown
- 2022-06-16 JP JP2023577551A patent/JP2024523343A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
CA3222578A1 (en) | 2022-12-22 |
KR20240021906A (ko) | 2024-02-19 |
WO2022264095A1 (en) | 2022-12-22 |
EP4355883A1 (en) | 2024-04-24 |
AU2022292197A1 (en) | 2023-12-21 |
JP2024523343A (ja) | 2024-06-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CL2018002825A1 (es) | Vacuna contra vrs | |
EA202190729A1 (ru) | Генетически сконструированная бактерия, содержащая энергогенерирующий ферментативный путь | |
BR112019022518A2 (pt) | Composto de dinucleotídeo cíclico bis 2'-5'-rr-(3'f-a)(3'f-a) e usos do mesmo | |
CL2018002920A1 (es) | Nuevos derivados de pirazolopirimidina | |
BR112019024830A2 (pt) | inibidores de bcl6 derivados de benzimidazolona | |
BR112017023975A2 (pt) | variantes de alfa-amilase e polinucleotídeos codificando as mesmas | |
GEP20227374B (en) | Novel, highly active pyrazolo-piperidine substituted indole-2-carboxamides active against the hepatitis b virus (hbv) | |
BR112013005872A2 (pt) | compostos, composição farmacêutica e respectivos usos | |
MX2020005458A (es) | Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. | |
BR112015023084A2 (pt) | anticorpo anti-cd25 monoclonal ou um fragmento ligante anti-cd25 de um anticorpo monoclonal, conjugado de anticorpo-droga, composição farmacêutica, ácido nucleico, vetor, célula hospedeira procariota e eucariota, método para a produção de um anticorpo anti-cd25 ou fragmento ligante anti-cd25, e, uso de um anticorpo anti-cd25 monoclonal de um conjugado anticorpo-droga ou de uma composição farmacêutica | |
BR112018010635A2 (pt) | linhagens celulares estáveis para a produção retroviral | |
AU2020317609A8 (en) | Novel heteroaryl-triazole compounds as pesticides | |
BR112012020693A8 (pt) | Derivados de ciclobutano e metilciclobutano como inibidores de janus quinase e composição que os compreende | |
PH12018500582A1 (en) | Crosslinked nucleic acid guna, method for producing same, and intermediate compound | |
CO2018006947A2 (es) | Novedoso bacteriófago pas-mup-1 de pasteurella multocida y su uso para inhibir la proliferación de pasteurella multocida | |
BR112017025923A2 (pt) | molécula de ácido nucleico recombinante, molécula de aminoácido recombinante, construto de expressão recombinante, vetor recombinante, micro-organismo recombinante, composição, métodos para produção de um micro-organismo recombinante e para cultivo e crescimento de um micro-organismo geneticamente modificado, uso de uma molécula de ácido nucleico recombinante, e, processo para produção fermentativa de um composto. | |
BR112018003154A2 (pt) | soluções de glicosídeo de esteviol | |
EP3929285A3 (en) | Methods of reducing odor | |
JOP20200323A1 (ar) | طرق لتصنيع تركيبات الأحماض الأمينية | |
BR112023026176A2 (pt) | Vetores de expressão, vetores livres de sequência bacteriana e métodos de fabricação e uso dos mesmos | |
MX2022000183A (es) | Lipidos cationicos y usos de estos. | |
CL2021001254A1 (es) | Vectores vegetales, composiciones y usos relacionados con los mismos | |
PH12020551035A1 (en) | Novel anthranilamides, their use as insecticide and processes for preparing the same | |
MX2020007631A (es) | Composiciones que contienen combinaciones de bacterias fijadoras de nitrogeno y agentes adicionales y su uso en la fijacion de nitrogeno en especies vegetales. | |
PE20211110A1 (es) | Distrofinas miniaturizadas y usos de las mismas |