BR112022020822A2 - Monitoramento ultravioleta de desempenho de cromatografia por quadrados mínimos parciais ortogonais - Google Patents

Monitoramento ultravioleta de desempenho de cromatografia por quadrados mínimos parciais ortogonais

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Publication number
BR112022020822A2
BR112022020822A2 BR112022020822A BR112022020822A BR112022020822A2 BR 112022020822 A2 BR112022020822 A2 BR 112022020822A2 BR 112022020822 A BR112022020822 A BR 112022020822A BR 112022020822 A BR112022020822 A BR 112022020822A BR 112022020822 A2 BR112022020822 A2 BR 112022020822A2
Authority
BR
Brazil
Prior art keywords
chromatography
squares
orthogonal partial
chromatogram
performance monitoring
Prior art date
Application number
BR112022020822A
Other languages
English (en)
Inventor
Shierly Eric
brennan Kevin
Mao Nathan
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of BR112022020822A2 publication Critical patent/BR112022020822A2/pt

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8693—Models, e.g. prediction of retention times, method development and validation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8658—Optimising operation parameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
    • G06F17/10—Complex mathematical operations
    • G06F17/18—Complex mathematical operations for evaluating statistical data, e.g. average values, frequency distributions, probability functions, regression analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8886—Analysis of industrial production processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/889—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 monitoring the quality of the stationary phase; column performance

Abstract

MONITORAMENTO ULTRAVIOLETA DE DESEMPENHO DE CROMATOGRAFIA POR QUADRADOS MÍNIMOS PARCIAIS ORTOGONAIS. A presente invenção refere-se a métodos para monitorar o desempenho de cromatografia em coluna. Os métodos podem incluir a aquisição de um ou mais traços ultravioletas de cromatograma (UV) gerados por um sistema de cromatografia durante purificação e/ou separação de amostra; e analisar o um ou mais traços de UV de cromatograma capturados com um modelo de quadrados mínimos parciais ortogonais (OPLS), permitindo, desse modo, a detecção da deterioração da coluna antes da falha da coluna e análise quantitativa do sinal UV nos um ou mais traços de UV de cromatograma.
BR112022020822A 2020-04-14 2021-04-13 Monitoramento ultravioleta de desempenho de cromatografia por quadrados mínimos parciais ortogonais BR112022020822A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063009835P 2020-04-14 2020-04-14
PCT/US2021/027043 WO2021211550A1 (en) 2020-04-14 2021-04-13 Ultraviolet monitoring of chromatography performance by orthogonal partial least squares

Publications (1)

Publication Number Publication Date
BR112022020822A2 true BR112022020822A2 (pt) 2022-11-29

Family

ID=75675062

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022020822A BR112022020822A2 (pt) 2020-04-14 2021-04-13 Monitoramento ultravioleta de desempenho de cromatografia por quadrados mínimos parciais ortogonais

Country Status (11)

Country Link
US (2) US11885779B2 (pt)
EP (1) EP4136442A1 (pt)
JP (1) JP2023522624A (pt)
KR (1) KR20220167303A (pt)
CN (1) CN115461620A (pt)
AU (1) AU2021256452A1 (pt)
BR (1) BR112022020822A2 (pt)
CA (1) CA3173338A1 (pt)
IL (1) IL297024A (pt)
MX (1) MX2022012872A (pt)
WO (1) WO2021211550A1 (pt)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US8410928B2 (en) * 2008-08-15 2013-04-02 Biogen Idec Ma Inc. Systems and methods for evaluating chromatography column performance
KR101747103B1 (ko) 2009-06-26 2017-06-14 리제너론 파마슈티칼스 인코포레이티드 천연 면역글로불린 포맷을 가지는 용이하게 분리된 이중특이성 항체
IL289895B (en) * 2016-10-25 2022-09-01 Regeneron Pharma Methods and systems for analysis of chromatography data
CN109115933B (zh) 2018-08-31 2020-10-23 中国人民解放军第四军医大学 一种筛选四逆汤抗心肌缺血药效物质基础的方法

Also Published As

Publication number Publication date
KR20220167303A (ko) 2022-12-20
MX2022012872A (es) 2022-11-08
IL297024A (en) 2022-12-01
CA3173338A1 (en) 2021-10-21
EP4136442A1 (en) 2023-02-22
CN115461620A (zh) 2022-12-09
WO2021211550A1 (en) 2021-10-21
JP2023522624A (ja) 2023-05-31
US11885779B2 (en) 2024-01-30
US20240118250A1 (en) 2024-04-11
AU2021256452A1 (en) 2022-11-10
US20210318275A1 (en) 2021-10-14

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