BR112022017180A2 - Células endoteliais da córnea humanas funcionais e aplicação das mesmas - Google Patents
Células endoteliais da córnea humanas funcionais e aplicação das mesmasInfo
- Publication number
- BR112022017180A2 BR112022017180A2 BR112022017180A BR112022017180A BR112022017180A2 BR 112022017180 A2 BR112022017180 A2 BR 112022017180A2 BR 112022017180 A BR112022017180 A BR 112022017180A BR 112022017180 A BR112022017180 A BR 112022017180A BR 112022017180 A2 BR112022017180 A2 BR 112022017180A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell
- functional
- corneal
- human corneal
- corneal endothelial
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
- A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases (EC 2.)
- C12N2501/727—Kinases (EC 2.7.)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Ophthalmology & Optometry (AREA)
- Immunology (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Reproductive Health (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Gynecology & Obstetrics (AREA)
- Hematology (AREA)
Abstract
CÉLULAS ENDOTELIAIS DA CÓRNEA HUMANAS FUNCIONAIS E APLICAÇÃO DAS MESMAS. A presente invenção refere-se a técnicas de ensaio de características celulares que identificam células do endotélio da córnea humanas cultivadas das quais a manifestação do efeito clínico precoce e um efeito clínico estável de longo prazo são confirmados, em ensaios clínicos. É provido um método de fabricação de uma célula endotelial corneana humana funcional capaz de eliciar uma função da córnea humana quando injetada dentro de uma câmara anterior de um olho humano, o método compreendendo a etapa de proliferar e/ou diferenciar ou amadurecer uma célula progenitora endotelial da córnea sob uma condição de cultura capaz de minimizar o estresse da cultura, como o estresse de proliferação. Além disso, é provida uma célula endotelial corneana humana funcional na qual a expressão de uma proteína funcional que leva a uma propriedade funcional do endotélio corneano (célula) levando à melhora da opacidade da córnea e edema hidratado, resultando na retenção contínua e de longo prazo da densidade da célula do tecido endotelial da córnea e a melhora na acuidade visual é reconhecida ou em que uma proteína que inibe a propriedade funcional do endotélio corneano (célula) não é desencadeada ou é reduzida.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020032139 | 2020-02-27 | ||
| PCT/JP2021/007490 WO2021172554A1 (ja) | 2020-02-27 | 2021-02-26 | ヒト機能性角膜内皮細胞およびその応用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112022017180A2 true BR112022017180A2 (pt) | 2022-10-18 |
Family
ID=77491963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112022017180A BR112022017180A2 (pt) | 2020-02-27 | 2021-02-26 | Células endoteliais da córnea humanas funcionais e aplicação das mesmas |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20230257700A1 (pt) |
| EP (1) | EP4116411A4 (pt) |
| JP (1) | JPWO2021172554A1 (pt) |
| KR (1) | KR20220146552A (pt) |
| CN (1) | CN115427551A (pt) |
| AU (1) | AU2021225617A1 (pt) |
| BR (1) | BR112022017180A2 (pt) |
| CA (1) | CA3173725A1 (pt) |
| WO (1) | WO2021172554A1 (pt) |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4678783B1 (en) | 1983-11-04 | 1995-04-04 | Asahi Chemical Ind | Substituted isoquinolinesulfonyl compounds |
| EP1195372A1 (en) | 1994-04-18 | 2002-04-10 | Mitsubishi Pharma Corporation | N-heterocyclic substituted benzamide derivatives with antihypertensive activity |
| JP3421217B2 (ja) | 1995-11-20 | 2003-06-30 | 麒麟麦酒株式会社 | Ｒｈｏ標的タンパク質Ｒｈｏキナーゼ |
| JP4316794B2 (ja) | 1997-10-22 | 2009-08-19 | 株式会社デ・ウエスタン・セラピテクス研究所 | イソキノリン誘導体及び医薬 |
| EP1094055B1 (en) | 1998-05-25 | 2006-04-26 | Santen Pharmaceutical Co., Ltd. | Novel vinylbenzene derivatives |
| ATE325795T1 (de) | 2001-03-23 | 2006-06-15 | Bayer Corp | Rho-kinase inhibitoren |
| PT1370552E (pt) | 2001-03-23 | 2007-04-30 | Bayer Pharmaceuticals Corp | Inibidores de rho-quinase |
| KR100883077B1 (ko) | 2001-04-11 | 2009-02-10 | 센주 세이야꾸 가부시키가이샤 | 시각 기능 장애 개선제 |
| EP1403255A4 (en) | 2001-06-12 | 2005-04-06 | Sumitomo Pharma | INHIBITORS OF RHO KINASE |
| US20040014755A1 (en) | 2002-01-10 | 2004-01-22 | Dhanapalan Nagarathnam | Rho-kinase inhibitors |
| WO2003062227A1 (en) | 2002-01-23 | 2003-07-31 | Bayer Pharmaceuticals Corporation | Rho-kinase inhibitors |
| JPWO2004009555A1 (ja) | 2002-07-22 | 2005-11-17 | 旭化成ファーマ株式会社 | ５−置換イソキノリン誘導体 |
| CA2400996A1 (en) | 2002-09-03 | 2004-03-03 | Lisa Mckerracher | 1,4-substituted cyclohexane derivatives |
| US7160894B2 (en) | 2003-06-06 | 2007-01-09 | Asahi Kasei Pharma Corporation | Tricyclic compound |
| CA2530389A1 (en) | 2003-07-02 | 2005-01-13 | Biofocus Discovery Limited | Pyrazine and pyridine derivatives as rho kinase inhibitors |
| WO2005039564A1 (en) | 2003-10-02 | 2005-05-06 | Vertex Pharmaceuticals Incorporated | Phthalimide compounds useful as protein kinase inhibitors |
| US7547779B2 (en) | 2003-10-06 | 2009-06-16 | Glaxo Group Limited | Preparation of 1,6-disubstituted azabenzimidazoles as kinase inhibitors |
| US20070123561A1 (en) | 2003-10-06 | 2007-05-31 | Dennis Lee | Preparation of 1,7-disubstituted azabensimidazoles as kinase inhibitors |
| US20070004771A1 (en) | 2003-10-06 | 2007-01-04 | Glaxo Group Limited | Preparation of 1,6,7-trisubstituted azabenzimidazoles as kinase inhibitors |
| JP2007015928A (ja) | 2003-10-15 | 2007-01-25 | Ube Ind Ltd | 新規オレフィン誘導体 |
| JP2007008816A (ja) | 2003-10-15 | 2007-01-18 | Ube Ind Ltd | 新規イソキノリン誘導体 |
| EP2468729B1 (en) | 2003-10-15 | 2013-12-25 | Ube Industries, Ltd. | Novel indazole derivative |
| EP1720874A4 (en) | 2004-02-24 | 2010-03-03 | Bioaxone Therapeutique Inc | SUBSTITUTED PIPERIDINE DERIVATIVES IN POSITION 4 |
| TW200604168A (en) | 2004-04-02 | 2006-02-01 | Vertex Pharma | Azaindoles useful as inhibitors of rock and other protein kinases |
| JPWO2006057270A1 (ja) | 2004-11-26 | 2008-06-05 | 旭化成ファーマ株式会社 | 含窒素３環化合物 |
| BRPI0614974A2 (pt) | 2005-08-30 | 2010-12-14 | Asahi Kasei Pharma Corp | composto, medicamento, inibidor da fosforilaÇço da cadeia leve regulatària da miosina, inibidor da via rho/rho quinase, e, mÉtodo para tratamento terapÊutico e/ou profilÁtico de glaucoma |
| EP2733201B1 (en) * | 2011-07-15 | 2018-02-21 | Osaka University | Method for preparing corneal endothelial cell |
| WO2013051722A1 (ja) | 2011-10-06 | 2013-04-11 | 学校法人 慶應義塾 | 角膜内皮細胞の製造方法 |
| JP6548576B2 (ja) * | 2013-07-30 | 2019-07-24 | 京都府公立大学法人 | 角膜内皮細胞マーカー |
| WO2016129654A1 (ja) * | 2015-02-12 | 2016-08-18 | 国立大学法人 東京大学 | 角膜内皮前駆細胞の調製方法 |
| JP7008337B2 (ja) * | 2016-02-15 | 2022-02-10 | 京都府公立大学法人 | ヒト機能性角膜内皮細胞およびその応用 |
| JP2020032139A (ja) | 2018-08-31 | 2020-03-05 | 株式会社三洋物産 | 遊技機 |
-
2021
- 2021-02-26 KR KR1020227033002A patent/KR20220146552A/ko unknown
- 2021-02-26 BR BR112022017180A patent/BR112022017180A2/pt unknown
- 2021-02-26 CN CN202180030379.9A patent/CN115427551A/zh active Pending
- 2021-02-26 US US17/802,824 patent/US20230257700A1/en active Pending
- 2021-02-26 AU AU2021225617A patent/AU2021225617A1/en active Pending
- 2021-02-26 WO PCT/JP2021/007490 patent/WO2021172554A1/ja unknown
- 2021-02-26 EP EP21761596.2A patent/EP4116411A4/en active Pending
- 2021-02-26 CA CA3173725A patent/CA3173725A1/en active Pending
- 2021-02-26 JP JP2022503767A patent/JPWO2021172554A1/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4116411A4 (en) | 2024-04-17 |
| EP4116411A1 (en) | 2023-01-11 |
| CA3173725A1 (en) | 2021-09-02 |
| WO2021172554A1 (ja) | 2021-09-02 |
| KR20220146552A (ko) | 2022-11-01 |
| JPWO2021172554A1 (pt) | 2021-09-02 |
| US20230257700A1 (en) | 2023-08-17 |
| AU2021225617A1 (en) | 2022-10-13 |
| CN115427551A (zh) | 2022-12-02 |
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