BR112022016948A2 - Enzimas para sialilação de glicanos - Google Patents
Enzimas para sialilação de glicanosInfo
- Publication number
- BR112022016948A2 BR112022016948A2 BR112022016948A BR112022016948A BR112022016948A2 BR 112022016948 A2 BR112022016948 A2 BR 112022016948A2 BR 112022016948 A BR112022016948 A BR 112022016948A BR 112022016948 A BR112022016948 A BR 112022016948A BR 112022016948 A2 BR112022016948 A2 BR 112022016948A2
- Authority
- BR
- Brazil
- Prior art keywords
- sialylation
- glycans
- enzymes
- nucleic acid
- methods
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01022—Lactose synthase (2.4.1.22)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01038—Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (2.4.1.38)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
- C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
ENZIMAS PARA SIALILAÇÃO DE GLICANOS. A presente invenção refere-se a proteínas de fusão, por exemplo, proteínas de fusão que compreendem uma porção(ões) enzimaticamente ativa(s) de ST6Gal1 ou B4GalT1, bem como métodos para produzir as mesmas, molécula(s) de ácido nucleico que codifica(m) a(s) proteína(s) de fusão, vetores que compreendem a(s) molécula(s) de ácido nucleico e célula(s) hospedeira(s) que compreende(m) o(s) vetor(es). A invenção também se refere a métodos de sialilação de anticorpos de imunoglobulina G (IgG).
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202062981293P | 2020-02-25 | 2020-02-25 | |
US202063026927P | 2020-05-19 | 2020-05-19 | |
PCT/US2021/019607 WO2021173797A1 (en) | 2020-02-25 | 2021-02-25 | Enzymes for sialylation of glycans |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112022016948A2 true BR112022016948A2 (pt) | 2022-11-22 |
Family
ID=77490192
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022016948A BR112022016948A2 (pt) | 2020-02-25 | 2021-02-25 | Enzimas para sialilação de glicanos |
Country Status (11)
Country | Link |
---|---|
US (1) | US20230303984A1 (pt) |
EP (1) | EP4110391A1 (pt) |
JP (1) | JP2023515554A (pt) |
KR (1) | KR20220145877A (pt) |
CN (1) | CN115605230A (pt) |
AU (1) | AU2021226352A1 (pt) |
BR (1) | BR112022016948A2 (pt) |
CA (1) | CA3173252A1 (pt) |
CO (1) | CO2022013638A2 (pt) |
MX (1) | MX2022010467A (pt) |
WO (1) | WO2021173797A1 (pt) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20240043838A (ko) * | 2022-09-27 | 2024-04-04 | 크리포 주식회사 | 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 비정형 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7094579B2 (en) * | 2002-02-13 | 2006-08-22 | Xoma Technology Ltd. | Eukaryotic signal sequences for prokaryotic expression |
ATE527371T1 (de) * | 2005-03-24 | 2011-10-15 | Biogenerix Ag | Expression löslicher, aktiver, eukaryotischer glucosyltransferasen in prokaryotischen organismen |
EP2900264A4 (en) * | 2012-09-26 | 2016-05-25 | Momenta Pharmaceuticals Inc | GLYKOPROTEINZUBEREITUNGEN |
EP2821482A1 (en) * | 2013-07-05 | 2015-01-07 | Roche Diagniostics GmbH | N-terminally truncated glycosyltransferases |
EP2824176A1 (en) * | 2013-07-11 | 2015-01-14 | Siamed'xpress | Methods for producing sialylated therapeutic proteins |
EP3083977B1 (en) * | 2013-12-20 | 2018-02-28 | F. Hoffmann-La Roche AG | Improved recombinant polypeptide production methods |
SG11201808457PA (en) * | 2016-04-15 | 2018-10-30 | Alpine Immune Sciences Inc | Icos ligand variant immunomodulatory proteins and uses thereof |
US11674125B2 (en) * | 2017-12-18 | 2023-06-13 | The General Hospital Corporation | Glycoengineering |
-
2021
- 2021-02-25 EP EP21760787.8A patent/EP4110391A1/en active Pending
- 2021-02-25 WO PCT/US2021/019607 patent/WO2021173797A1/en unknown
- 2021-02-25 US US17/802,441 patent/US20230303984A1/en active Pending
- 2021-02-25 MX MX2022010467A patent/MX2022010467A/es unknown
- 2021-02-25 KR KR1020227033078A patent/KR20220145877A/ko unknown
- 2021-02-25 CN CN202180030801.0A patent/CN115605230A/zh active Pending
- 2021-02-25 BR BR112022016948A patent/BR112022016948A2/pt unknown
- 2021-02-25 CA CA3173252A patent/CA3173252A1/en active Pending
- 2021-02-25 AU AU2021226352A patent/AU2021226352A1/en active Pending
- 2021-02-25 JP JP2022551014A patent/JP2023515554A/ja active Pending
-
2022
- 2022-09-22 CO CONC2022/0013638A patent/CO2022013638A2/es unknown
Also Published As
Publication number | Publication date |
---|---|
AU2021226352A1 (en) | 2022-09-29 |
KR20220145877A (ko) | 2022-10-31 |
EP4110391A1 (en) | 2023-01-04 |
JP2023515554A (ja) | 2023-04-13 |
CO2022013638A2 (es) | 2022-10-21 |
US20230303984A1 (en) | 2023-09-28 |
CN115605230A (zh) | 2023-01-13 |
CA3173252A1 (en) | 2021-09-02 |
WO2021173797A1 (en) | 2021-09-02 |
MX2022010467A (es) | 2022-12-15 |
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