BR112022012881A2 - MICROFLUID CHIP, A METHOD OF FOCUSING PARTICLES IN A FLOW OF FLUID AND A METHOD FOR PRODUCING A FLUID WITH GENDER-BIASED SPERM CELLS - Google Patents

MICROFLUID CHIP, A METHOD OF FOCUSING PARTICLES IN A FLOW OF FLUID AND A METHOD FOR PRODUCING A FLUID WITH GENDER-BIASED SPERM CELLS

Info

Publication number
BR112022012881A2
BR112022012881A2 BR112022012881A BR112022012881A BR112022012881A2 BR 112022012881 A2 BR112022012881 A2 BR 112022012881A2 BR 112022012881 A BR112022012881 A BR 112022012881A BR 112022012881 A BR112022012881 A BR 112022012881A BR 112022012881 A2 BR112022012881 A2 BR 112022012881A2
Authority
BR
Brazil
Prior art keywords
fluid
flow
sperm cells
gender
producing
Prior art date
Application number
BR112022012881A
Other languages
Portuguese (pt)
Inventor
Xia Zheng
Kamalakshakurup Gopakumar
Original Assignee
Abs Global Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abs Global Inc filed Critical Abs Global Inc
Publication of BR112022012881A2 publication Critical patent/BR112022012881A2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06—Investigating concentration of particle suspensions
    • G01N15/075—Investigating concentration of particle suspensions by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1413—Hydrodynamic focussing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

CHIP MICROFLUÍDICO, MÉTODO DE FOCAGEM DE PARTÍCULAS EM UM FLUXO DE FLUIDO E MÉTODO PARA PRODUZIR UM FLUIDO COM CÉLULAS DE ESPERMA ENVIESADAS POR GÊNERO. Dispositivos microfluídicos e métodos para focar componentes em uma amostra de fluido são descritos aqui. Os dispositivos microfluídicos apresentam um chip microfluídico tendo um microcanal tendo uma porção constritiva que se estreita em largura e uma região de focagem de fluxo a jusante do microcanal. A região de focagem de fluxo inclui uma superfície inferior positivamente inclinada que reduz uma altura da região de focagem de fluxo e paredes laterais que se afunilam para reduzir uma largura da região de focagem de fluxo, deste modo constringindo geometricamente a região de focagem de fluxo. Os dispositivos e métodos podem ser utilizados na triagem por sexo de células de esperma para melhorar o desempenho e aumentar a elegibilidade.MICROFLUID CHIP, METHOD OF FOCUSING PARTICLES IN A FLOW OF FLUID AND METHOD FOR PRODUCING A FLUID WITH GENDER-BIDDED SPERM CELLS. Microfluidic devices and methods for focusing components in a fluid sample are described here. Microfluidic devices feature a microfluidic chip having a microchannel having a constricting portion that narrows in width and a flow focusing region downstream of the microchannel. The flux focusing region includes a positively sloped bottom surface that reduces a height of the flux focusing region and side walls that taper to reduce a width of the flux focusing region, thereby geometrically constricting the flux focusing region. The devices and methods can be used in sex screening of sperm cells to improve performance and increase eligibility.

BR112022012881A 2020-01-13 2020-01-13 MICROFLUID CHIP, A METHOD OF FOCUSING PARTICLES IN A FLOW OF FLUID AND A METHOD FOR PRODUCING A FLUID WITH GENDER-BIASED SPERM CELLS BR112022012881A2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2020/013392 WO2021145854A1 (en) 2020-01-13 2020-01-13 Single-sheath microfluidic chip

Publications (1)

Publication Number Publication Date
BR112022012881A2 true BR112022012881A2 (en) 2022-09-06

Family

ID=76864024

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022012881A BR112022012881A2 (en) 2020-01-13 2020-01-13 MICROFLUID CHIP, A METHOD OF FOCUSING PARTICLES IN A FLOW OF FLUID AND A METHOD FOR PRODUCING A FLUID WITH GENDER-BIASED SPERM CELLS

Country Status (5)

Country Link
EP (1) EP4090932A4 (en)
JP (1) JP2023514941A (en)
CN (1) CN115023599A (en)
BR (1) BR112022012881A2 (en)
WO (1) WO2021145854A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201608159XA (en) * 2012-02-29 2016-11-29 Fluidigm Corp Methods, systems, and devices for multiple single-cell capturing and processing using microfluidics
US9757726B2 (en) * 2013-03-14 2017-09-12 Inguran, Llc System for high throughput sperm sorting
US11796449B2 (en) * 2013-10-30 2023-10-24 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
NZ740697A (en) * 2015-09-30 2022-07-01 Semen Refinement B V Microfluidic device for selection of semen

Also Published As

Publication number Publication date
CN115023599A (en) 2022-09-06
JP2023514941A (en) 2023-04-12
WO2021145854A1 (en) 2021-07-22
EP4090932A1 (en) 2022-11-23
EP4090932A4 (en) 2023-10-18

Similar Documents

Publication Publication Date Title
CA2966603C (en) Combined sorting and concentrating particles in a microfluidic device
Zhou et al. Enhanced size-dependent trapping of particles using microvortices
KR100508326B1 (en) Cascaded hydrodynamic focusing in microfluidic channels
CN104225964B (en) Microfluid removal of bubbles device and preparation method thereof and microfluidic device
Zhou et al. Hybrid microfluidic sorting of rare cells based on high throughput inertial focusing and high accuracy acoustic manipulation
Tan et al. Continuous separation of white blood cells from whole blood using viscoelastic effects
SG149889A1 (en) Multilayer hydrodynamic sheath flow structure
US10688490B2 (en) Microfluidic device
Gao et al. Inertial lateral migration and self-assembly of particles in bidisperse suspensions in microchannel flows
CN106422924B (en) Square wave passive micro mixer
CN105032518B (en) Micro-fluidic chip heat abstractor and preparation method thereof
BR112022012881A2 (en) MICROFLUID CHIP, A METHOD OF FOCUSING PARTICLES IN A FLOW OF FLUID AND A METHOD FOR PRODUCING A FLUID WITH GENDER-BIASED SPERM CELLS
Huang et al. Enhanced efficiency of sorting sperm motility utilizing a microfluidic chip
Liu et al. Hydrodynamic separation by changing equilibrium positions in contraction–expansion array channels
CN107159326A (en) Inertia based on enhanced Secondary Flow focuses on chip
US20100059120A1 (en) Microfluidic device and methods for droplet generation and manipulation
WO2022154755A1 (en) Methods and devices for separation of motile sperm
Suwannaphan et al. Investigation of shear stress and cell survival in a microfluidic chip for a single cell study
GAO et al. Analysis of the Inhibitory Effect of Tirofiban on Shear-Induced Platelet Aggregation by Microfluidic Technique
DK173796B1 (en) Method for controlling flow in a flow system
CN114643088B (en) Micro-droplet generation chip based on karman vortex street
CN114713302B (en) Microfluidic chip and application thereof
Mane et al. Effect of Newtonian and shear thinning medium on human sperm motion within a microchannel
CN108855267B (en) Micro-channel platform for micro-control of biological micro-nano particles
JP2020025900A (en) Particle separation method