BR112022007541A2 - Organismo microbiano de ocorrência não natural e métodos para aumentar a disponibilidade de nadph - Google Patents
Organismo microbiano de ocorrência não natural e métodos para aumentar a disponibilidade de nadphInfo
- Publication number
- BR112022007541A2 BR112022007541A2 BR112022007541A BR112022007541A BR112022007541A2 BR 112022007541 A2 BR112022007541 A2 BR 112022007541A2 BR 112022007541 A BR112022007541 A BR 112022007541A BR 112022007541 A BR112022007541 A BR 112022007541A BR 112022007541 A2 BR112022007541 A2 BR 112022007541A2
- Authority
- BR
- Brazil
- Prior art keywords
- methods
- nadph
- availability
- increase
- exogenous nucleic
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/36—Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01036—Acetoacetyl-CoA reductase (1.1.1.36)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/01009—Glyceraldehyde-3-phosphate dehydrogenase (NADP+) (1.2.1.9)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/01—Oxidoreductases acting on NADH or NADPH (1.6) with NAD+ or NADP+ as acceptor (1.6.1)
- C12Y106/01002—NAD(P)+ Transhydrogenase (AB-specific) (1.6.1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01023—NAD+ kinase (2.7.1.23)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
ORGANISMO MICROBIANO DE OCORRÊNCIA NÃO NATURAL E MÉTODOS PARA AUMENTAR A DISPONIBILIDADE DE NADPH. A presente invenção fornece organismos microbianos que têm disponibilidade aumentada de cofatores, tais como NADPH, para aumentar a produção de vários produtos, incluindo 1,3-BDO, MMA, (3R)-hidroxibutil (3R)-hidroxibutirato, aminoácidos, 3HB-CoA, adipato, caprolactama, 6-ACA, HMDA ou MAA e produtos feitos a partir de qualquer um destes. Também são fornecidos um ou mais ácidos nucleicos exógenos que codificam uma enzima expressa em uma quantidade suficiente para aumentar a disponibilidade de NADPH, em que o ácido nucleico exógeno inclui um ou mais dentre ATP-NADH quinase, pntAB, nadK e gapN. Também são fornecidas uma ou mais atenuações de genes ocorrendo em genes, tais como NDH-2, que resultam em uma razão aumentada de NADPH para NADH. Várias combinações dos ácidos nucleicos exógenos e deleções de genes também são fornecidas na presente invenção. A presente invenção também fornece métodos para fabricar e usar os mesmos, incluindo métodos para cultivar células e para a produção dos vários produtos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962925150P | 2019-10-23 | 2019-10-23 | |
PCT/US2020/056831 WO2021081185A1 (en) | 2019-10-23 | 2020-10-22 | Microorganisms and methods for increasing co-factors |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112022007541A2 true BR112022007541A2 (pt) | 2022-07-12 |
Family
ID=75620354
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022007541A BR112022007541A2 (pt) | 2019-10-23 | 2020-10-22 | Organismo microbiano de ocorrência não natural e métodos para aumentar a disponibilidade de nadph |
Country Status (7)
Country | Link |
---|---|
US (1) | US20220403420A1 (pt) |
EP (1) | EP4048781A1 (pt) |
KR (1) | KR20220085818A (pt) |
CN (1) | CN114901815A (pt) |
BR (1) | BR112022007541A2 (pt) |
CA (1) | CA3158515A1 (pt) |
WO (1) | WO2021081185A1 (pt) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA3225234A1 (en) * | 2022-08-04 | 2024-02-08 | Metabolic Explorer | Microorganism and method for the improved production of serine and/or cysteine |
WO2024084049A2 (en) | 2022-10-20 | 2024-04-25 | Cysbio Aps | Genetically modified host cells producing l-serine |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080293101A1 (en) * | 2006-07-27 | 2008-11-27 | Peters Matthew W | Engineered microorganisms for increasing product yield in biotransformations, related methods and systems |
WO2009144245A1 (en) * | 2008-05-28 | 2009-12-03 | Novozymes A/S | Method for increasing growth rate |
US20120190089A1 (en) * | 2008-10-31 | 2012-07-26 | California Institute Of Technology | Engineered microogranisms capable of producing target compounds under anaerobic conditions |
KR101261147B1 (ko) * | 2011-01-18 | 2013-05-06 | 씨제이제일제당 (주) | L-아미노산의 생산능이 향상된 미생물 및 이를 이용하여 l-아미노산을 생산하는 방법 |
CN106559997B (zh) * | 2014-05-12 | 2021-04-09 | 代谢探索者公司 | 基于nadph依赖型丙酮醇还原酶和改进的nadph供给用于生产1,2-丙二醇的新微生物和方法 |
-
2020
- 2020-10-22 BR BR112022007541A patent/BR112022007541A2/pt unknown
- 2020-10-22 WO PCT/US2020/056831 patent/WO2021081185A1/en unknown
- 2020-10-22 US US17/770,233 patent/US20220403420A1/en active Pending
- 2020-10-22 KR KR1020227017292A patent/KR20220085818A/ko unknown
- 2020-10-22 EP EP20878843.0A patent/EP4048781A1/en active Pending
- 2020-10-22 CN CN202080090024.4A patent/CN114901815A/zh active Pending
- 2020-10-22 CA CA3158515A patent/CA3158515A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA3158515A1 (en) | 2021-04-29 |
CN114901815A (zh) | 2022-08-12 |
EP4048781A1 (en) | 2022-08-31 |
US20220403420A1 (en) | 2022-12-22 |
KR20220085818A (ko) | 2022-06-22 |
WO2021081185A1 (en) | 2021-04-29 |
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