BR112022000061A2 - Método para o cultivo de células aderentes em um bioreator multiparalelo - Google Patents

Método para o cultivo de células aderentes em um bioreator multiparalelo

Info

Publication number
BR112022000061A2
BR112022000061A2 BR112022000061A BR112022000061A BR112022000061A2 BR 112022000061 A2 BR112022000061 A2 BR 112022000061A2 BR 112022000061 A BR112022000061 A BR 112022000061A BR 112022000061 A BR112022000061 A BR 112022000061A BR 112022000061 A2 BR112022000061 A2 BR 112022000061A2
Authority
BR
Brazil
Prior art keywords
adherent cells
multiparallel
bioreactor
cultivating
cultivating adherent
Prior art date
Application number
BR112022000061A
Other languages
English (en)
Inventor
Berrie Dalton
Green April
Vela Eric
Original Assignee
Ology Bioservices Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ology Bioservices Inc filed Critical Ology Bioservices Inc
Publication of BR112022000061A2 publication Critical patent/BR112022000061A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Abstract

método para o cultivo de células aderentes em um bioreator multiparalelo. a invenção refere-se a um método para o cultivo de células aderentes em um biorreator multiparalelo, utilizando fitas de pet veículo, para a otimização dos parâmetros de crescimento e produção de células aderentes. a invenção refere-se também a um método para a propagação de vírus e vetores a partir de células aderentes em um biorreator multiparalelo para a otimização do processo de produção.
BR112022000061A 2019-07-01 2020-06-30 Método para o cultivo de células aderentes em um bioreator multiparalelo BR112022000061A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962869050P 2019-07-01 2019-07-01
PCT/US2020/040359 WO2021003188A1 (en) 2019-07-01 2020-06-30 Method for cultivation of adherent cells in a multiparallel bioreactor

Publications (1)

Publication Number Publication Date
BR112022000061A2 true BR112022000061A2 (pt) 2022-05-24

Family

ID=74100625

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022000061A BR112022000061A2 (pt) 2019-07-01 2020-06-30 Método para o cultivo de células aderentes em um bioreator multiparalelo

Country Status (11)

Country Link
US (1) US20220186167A1 (pt)
EP (1) EP3994246A4 (pt)
JP (1) JP2022539210A (pt)
KR (1) KR20220075305A (pt)
CN (1) CN114599782A (pt)
AU (1) AU2020300517A1 (pt)
BR (1) BR112022000061A2 (pt)
CA (1) CA3144960A1 (pt)
IL (1) IL289454A (pt)
MX (1) MX2022000002A (pt)
WO (1) WO2021003188A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3230028A1 (en) 2019-06-17 2020-12-24 Deka Products Limited Partnership System and method for cell and tissue preparation
CN117402835A (zh) * 2023-12-11 2024-01-16 深圳源兴基因技术有限公司 一种痘苗病毒的高产培养方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070178586A1 (en) * 2005-11-09 2007-08-02 The Ohio State University Research Foundation Methods and apparatuses for growing cells
KR101504392B1 (ko) * 2008-11-03 2015-03-19 크루셀 홀란드 비.브이. 아데노바이러스 벡터의 제조방법
US9399755B2 (en) * 2010-05-11 2016-07-26 Pall Artelis Apparatus and methods for cell culture
US20120156772A1 (en) * 2010-12-16 2012-06-21 General Electric Company Cell carrier and methods of making
CN107794248A (zh) * 2017-10-24 2018-03-13 广州齐志生物工程设备有限公司 一种用微载体悬浮培养犬瘟热病毒的方法
SG11202005815UA (en) * 2017-12-20 2020-07-29 Univercells Tech S A Bioreactor and related methods

Also Published As

Publication number Publication date
CN114599782A (zh) 2022-06-07
IL289454A (en) 2022-02-01
EP3994246A1 (en) 2022-05-11
US20220186167A1 (en) 2022-06-16
WO2021003188A1 (en) 2021-01-07
EP3994246A4 (en) 2023-10-04
JP2022539210A (ja) 2022-09-07
AU2020300517A1 (en) 2022-02-03
KR20220075305A (ko) 2022-06-08
MX2022000002A (es) 2022-03-17
CA3144960A1 (en) 2021-01-07

Similar Documents

Publication Publication Date Title
BR112022000061A2 (pt) Método para o cultivo de células aderentes em um bioreator multiparalelo
CY1123146T1 (el) Καλλιεργεια κυτταρου θηλαστικου
MY162865A (en) Cultivation, harvesting and processing of floating aquatic species with high growth rates
AR073113A1 (es) Produccion de metionina sin n- acil -metionina
PH12016502148A1 (en) Methods of microalgae cultivation for increased resource production
NZ597742A (en) Method of producing a polypeptide or virus of interest in a continuous cell culture
BR112015011722A2 (pt) produção de altos rendimentos de polissacarídeos bacterianos
WO2017051347A3 (en) Cells and method of cell culture
EA201592169A1 (ru) Рекомбинантные микроорганизмы, приводящие к увеличению потока через путь ферментации
WO2012014207A3 (en) Method for generating induced pluripotent stem cells from keratinocytes derived from plucked hair follicles
BR112016010340A2 (pt) novos métodos para se produzir tert ativa
WO2018203947A3 (en) MODIFIED BIOSYNTHESIS PATHWAYS FOR FERMENTATION TYRAMINE PRODUCTION
MX2012007117A (es) Metodo para cultivar celulas adherentes.
WO2018226170A3 (en) MUTANT STRAIN OF CLOSTRIDIUM THERMOCELLUM FOR THE PRODUCTION OF CELLULASE AND XYLANASE AND PREPARATION METHOD THEREOF
EP3868863A4 (en) CELL CULTURE SUBSTRATE, METHOD FOR PREPARING A CELL CULTURE SUBSTRATE, AND METHOD FOR PREPARING SPHEROIDS
BR112016016585A2 (pt) processo para a produção de açúcares solúveis a partir de biomassa
MX358824B (es) Proceso de produccion de un coctel enzimatico utilizando los residuos liquidos de un proceso de conversion bioquimica de materiales ligno-celulosicos.
IN2014CN01889A (pt)
CA2865497A1 (en) Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation
CL2023000576A1 (es) Estrategias de suministro con asparagina para mejorar el rendimiento del cultivo celular y mitigar las variantes de secuencia de asparagina.
BR112017017303B8 (pt) Método para obtenção de clones de células de alta produtividade
MX2020005183A (es) Procedimientos de alta productividad para cultivar algas.
SG10201811177SA (en) Cell culture methods and media comprising n-acetylcysteine
Fang et al. Effects of irrigation amount and various fertigation methods on yield and quality of cucumber in greenhouse
EP3943555A4 (en) CELL CULTURE CARRIER AND METHOD AND DEVICE FOR ITS PRODUCTION