BR112021025372A2 - Células hospedeiras modificadas para produção de vanilina de alta eficiência - Google Patents

Células hospedeiras modificadas para produção de vanilina de alta eficiência

Info

Publication number
BR112021025372A2
BR112021025372A2 BR112021025372A BR112021025372A BR112021025372A2 BR 112021025372 A2 BR112021025372 A2 BR 112021025372A2 BR 112021025372 A BR112021025372 A BR 112021025372A BR 112021025372 A BR112021025372 A BR 112021025372A BR 112021025372 A2 BR112021025372 A2 BR 112021025372A2
Authority
BR
Brazil
Prior art keywords
host cells
efficiency
modified host
vanillin
production
Prior art date
Application number
BR112021025372A
Other languages
English (en)
Portuguese (pt)
Inventor
Chad Hansen
Lauren Raetz
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of BR112021025372A2 publication Critical patent/BR112021025372A2/pt

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C47/00Compounds having —CHO groups
    • C07C47/52Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings
    • C07C47/575Compounds having —CHO groups bound to carbon atoms of six—membered aromatic rings containing ether groups, groups, groups, or groups
    • C07C47/58Vanillin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/46Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/24Preparation of oxygen-containing organic compounds containing a carbonyl group
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01003Aldehyde dehydrogenase (NAD+) (1.2.1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
BR112021025372A 2019-08-01 2020-07-31 Células hospedeiras modificadas para produção de vanilina de alta eficiência BR112021025372A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962881874P 2019-08-01 2019-08-01
PCT/US2020/044613 WO2021022216A1 (en) 2019-08-01 2020-07-31 Modified host cells for high efficiency production of vanillin

Publications (1)

Publication Number Publication Date
BR112021025372A2 true BR112021025372A2 (pt) 2022-02-08

Family

ID=72179198

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021025372A BR112021025372A2 (pt) 2019-08-01 2020-07-31 Células hospedeiras modificadas para produção de vanilina de alta eficiência

Country Status (8)

Country Link
US (1) US12480101B2 (https=)
EP (1) EP4007815A1 (https=)
JP (1) JP7728744B2 (https=)
CN (1) CN114174489B (https=)
BR (1) BR112021025372A2 (https=)
CA (1) CA3147424A1 (https=)
MX (1) MX2022001073A (https=)
WO (1) WO2021022216A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230175023A1 (en) * 2021-02-18 2023-06-08 Zymergen Inc. Engineered biosynthetic pathways for production of 3,4-dihydroxybenzoic acid by fermentation
WO2023159017A1 (en) 2022-02-15 2023-08-24 Evolva Sa Process for recovering and purifying vanillin
CN114790472B (zh) * 2022-03-25 2024-04-16 上海龙殷生物科技有限公司 一种果糖糖基化的姜黄素、制备方法及应用
CN117004544B (zh) * 2023-09-14 2023-12-12 北京化工大学 一种以葡萄糖为底物从头合成香兰素的基因工程菌及其应用
KR20250098104A (ko) * 2023-12-21 2025-07-01 대상 주식회사 1,4-부탄디올 생산능이 향상된 변이 미생물 및 이를 이용한 1,4-부탄디올의 생산 방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2344665A1 (en) 1998-09-18 2000-03-30 Board Of Trustees Operating Michigan State University Synthesis of vanillin from a carbon source
US20140017748A1 (en) * 2010-02-12 2014-01-16 Gevo, Inc. Modified alcohol dehydrogenases for the production of fuels and chemicals
CN108570464B (zh) * 2011-08-08 2021-12-21 国际香料香精公司 用于香草醛或香草醛β-D-葡萄糖苷的生物合成的组合物和方法
US10000782B2 (en) * 2013-07-16 2018-06-19 International Flavors & Fragrances Inc. Recombinant host cell for the biosynthesis of vanillin or vanillin beta-D-glucoside
BR112016002526B1 (pt) 2013-08-07 2021-11-23 Total Marketing Services Método para produção de um composto heterólogo não catabólico, e, composição de fermentação
US20170172184A1 (en) 2014-02-12 2017-06-22 Evolva Sa Methods of Improving Production of Vanillin
EP2957629A1 (en) * 2014-06-18 2015-12-23 Rhodia Opérations Improved production of vanilloids by fermentation
WO2016044713A1 (en) * 2014-09-18 2016-03-24 Genomatica, Inc. Non-natural microbial organisms with improved energetic efficiency
EP4134442A1 (en) 2015-05-29 2023-02-15 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high ph
US10808015B2 (en) 2015-06-25 2020-10-20 Amyris, Inc. Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds

Also Published As

Publication number Publication date
MX2022001073A (es) 2022-02-14
CA3147424A1 (en) 2021-02-04
JP2022542228A (ja) 2022-09-30
JP7728744B2 (ja) 2025-08-25
CN114174489A (zh) 2022-03-11
US12480101B2 (en) 2025-11-25
WO2021022216A1 (en) 2021-02-04
US20220282226A1 (en) 2022-09-08
CN114174489B (zh) 2024-11-08
EP4007815A1 (en) 2022-06-08

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