BR112021024174A2 - Sequenciamento flexível e de alto rendimento de regiões genômicas alvo - Google Patents

Sequenciamento flexível e de alto rendimento de regiões genômicas alvo

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Publication number
BR112021024174A2
BR112021024174A2 BR112021024174A BR112021024174A BR112021024174A2 BR 112021024174 A2 BR112021024174 A2 BR 112021024174A2 BR 112021024174 A BR112021024174 A BR 112021024174A BR 112021024174 A BR112021024174 A BR 112021024174A BR 112021024174 A2 BR112021024174 A2 BR 112021024174A2
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BR
Brazil
Prior art keywords
probe
target genomic
genomic region
extension
flexible
Prior art date
Application number
BR112021024174A
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English (en)
Inventor
Adam Payton
Gomide Neves Leandro
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Rapid Genomics Llc
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Publication date
Application filed by Rapid Genomics Llc filed Critical Rapid Genomics Llc
Publication of BR112021024174A2 publication Critical patent/BR112021024174A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

sequenciamento flexível e de alto rendimento de regiões genômicas alvo. a divulgação refere-se a materiais e métodos para capturar uma região genômica alvo, compreendendo a hibridização de uma sonda de extensão e uma sonda de ligação para sequências alvo que flanqueiam a região genômica alvo; alongamento da extremidade 3' da extensão sonda até que a extremidade 3' da sonda de extensão alongada esteja adjacente à extremidade 5' da sonda de ligação; e ligando a extremidade 3' do sonda de extensão alongada com a extremidade 5' da sonda de ligação para produzir uma sonda ligada. a sonda ligada pode ser amplificada por pcr para produzir cópias da região genômica alvo que podem ser detectadas ou sequenciadas. certas modalidades da invenção também fornecer métodos de produção de sondas de fita dupla adequadas para capturar e analisar ambas as fitas de uma região genômica alvo em um dna genômico de fita dupla. a invenção também fornece kits para realizar os métodos aqui divulgados.
BR112021024174A 2019-05-30 2020-05-29 Sequenciamento flexível e de alto rendimento de regiões genômicas alvo BR112021024174A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962854458P 2019-05-30 2019-05-30
PCT/US2020/035365 WO2020243597A1 (en) 2019-05-30 2020-05-29 Flexible and high-throughput sequencing of targeted genomic regions

Publications (1)

Publication Number Publication Date
BR112021024174A2 true BR112021024174A2 (pt) 2022-04-26

Family

ID=73551132

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021024174A BR112021024174A2 (pt) 2019-05-30 2020-05-29 Sequenciamento flexível e de alto rendimento de regiões genômicas alvo

Country Status (11)

Country Link
US (2) US11168367B2 (pt)
EP (1) EP3976899A4 (pt)
JP (1) JP2022535029A (pt)
KR (1) KR20220038604A (pt)
CN (1) CN114207229A (pt)
AU (1) AU2020283039A1 (pt)
BR (1) BR112021024174A2 (pt)
CA (1) CA3142010A1 (pt)
CL (1) CL2021003171A1 (pt)
MX (1) MX2021014653A (pt)
WO (1) WO2020243597A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3969581A4 (en) * 2019-05-13 2023-06-14 Rapid Genomics LLC DETECTION AND ANALYSIS OF TARGET GENOMIC REGIONS
WO2022200793A1 (en) * 2021-03-25 2022-09-29 The University Of Birmingham Analyte capture

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Publication number Priority date Publication date Assignee Title
GB8311018D0 (en) 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
DE69943392D1 (de) * 1998-08-20 2011-06-09 Du Pont Gene für fettsäuremodfizierende enzyme von planzen und damit verbundene bildung von konjugierten doppelbindungen
NZ514569A (en) * 1999-03-02 2004-02-27 Invitrogen Corp Compositions and methods for use in recombinational cloning of nucleic acids
US20020086289A1 (en) 1999-06-15 2002-07-04 Don Straus Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
EP1602733A1 (en) 2004-06-02 2005-12-07 Keygene N.V. Detection of target nucleotide sequences using an asymmetric oligonucleotide ligation assay
US8808991B2 (en) 2003-09-02 2014-08-19 Keygene N.V. Ola-based methods for the detection of target nucleic avid sequences
JP5237126B2 (ja) 2006-03-01 2013-07-17 キージーン ナムローゼ フェンノートシャップ ライゲーションアッセイを用いてハイスループットシークエンスに基づき遺伝子関連配列を検出する方法
US7897747B2 (en) 2006-05-25 2011-03-01 The Board Of Trustees Of The Leland Stanford Junior University Method to produce single stranded DNA of defined length and sequence and DNA probes produced thereby
KR100843133B1 (ko) 2006-09-20 2008-07-02 삼성전자주식회사 플래시 메모리에서 매핑 정보 재구성을 위한 장치 및 방법
US20090156412A1 (en) 2007-12-17 2009-06-18 Helicos Biosciences Corporation Surface-capture of target nucleic acids
US8986958B2 (en) * 2009-03-30 2015-03-24 Life Technologies Corporation Methods for generating target specific probes for solution based capture
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BR112013033462A2 (pt) 2011-06-27 2017-03-01 Univ Florida método para a redução de complexidade do genoma e detecção de polimorfismo
US9476089B2 (en) * 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
US20150141257A1 (en) 2013-08-02 2015-05-21 Roche Nimblegen, Inc. Sequence capture method using specialized capture probes (heatseq)
WO2018231985A1 (en) * 2017-06-13 2018-12-20 Genetics Research, Llc, D/B/A Zs Genetics, Inc. Polynucleic acid molecule enrichment methodologies
CN110079592B (zh) * 2018-01-26 2021-02-12 厦门艾德生物医药科技股份有限公司 用于检测基因突变和已知、未知基因融合类型的高通量测序靶向捕获目标区域的探针和方法

Also Published As

Publication number Publication date
AU2020283039A1 (en) 2022-01-20
EP3976899A4 (en) 2023-10-04
CA3142010A1 (en) 2020-12-03
CL2021003171A1 (es) 2023-03-03
MX2021014653A (es) 2022-04-06
US11168367B2 (en) 2021-11-09
WO2020243597A1 (en) 2020-12-03
US20200377943A1 (en) 2020-12-03
EP3976899A1 (en) 2022-04-06
KR20220038604A (ko) 2022-03-29
JP2022535029A (ja) 2022-08-04
CN114207229A (zh) 2022-03-18
US20220042096A1 (en) 2022-02-10

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