BR112021011221A2 - Métodos e composições para tratar hiperoxalúria - Google Patents

Métodos e composições para tratar hiperoxalúria Download PDF

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BR112021011221A2
BR112021011221A2 BR112021011221-9A BR112021011221A BR112021011221A2 BR 112021011221 A2 BR112021011221 A2 BR 112021011221A2 BR 112021011221 A BR112021011221 A BR 112021011221A BR 112021011221 A2 BR112021011221 A2 BR 112021011221A2
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identity
nucleotide sequence
bacterium
oxalate
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Zachary Nicholas Russ, Iv
Weston Robert Whitaker
William Cain DeLoache
Elizabeth Joy Stanley Shepherd
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Novome Biotechnologies, Inc.
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Abstract

métodos e composições para tratar hiperoxalúria. a divulgação se refere geralmente às bactérias que foram modificadas para ter atividade degradante de oxalato aumentada, composições farmacêuticas incluindo as bactérias e métodos de tratar transtornos associados com uma quantidade elevada de oxalato, e.g., hiperoxalúria.

Description

“MÉTODOS E COMPOSIÇÕES PARA TRATAR HIPEROXALÚRIA” REFERÊNCIA CRUZADA AOS PEDIDOS RELACIONADOS
[001] Este pedido reivindica o benefício e prioridade do pedido de patente provisória U.S. número de série 62/777.322, depositado em 10 de dezembro de 2018, que é aqui incorporado por referência em sua totalidade.
FUNDAMENTOS
[002] O ácido oxálico, o menor ácido dicarboxílico, frequentemente aparece na natureza como um metabólito secundário útil e também um subproduto de processos catabólicos. As plantas produzem e acumulam grandes quantidades de oxalato como uma forma de envenenar os herbívoros e evitar a predação. Vários processos metabólicos também geram oxalato dentro dos corpos de mamíferos. O oxalato tóxico deve ser eliminado e, em mamíferos, isso é feito pelo transporte de oxalato na urina e fezes. Entretanto, se muito oxalato for eliminado na urina, pode ocorrer hiperoxalúria.
[003] A hiperoxalúria é a condição de excretar o excesso de oxalato na urina. O oxalato urinário elevado tem sido associado a cálculos renais de oxalato de cálcio recorrentes, danos renais e, eventualmente, doença renal em estágio terminal. A hiperoxalúria é categorizada em quatro classes pela principal fonte do excesso oxalato: hiperoxalúria primária, entérica, dietética e idiopática. A hiperoxalúria primária diz respeito à hiperoxalúria onde as causas são genéticas; suas três formas, I, II e III, são causadas por mutações nos genes humanos AGXT, GRHPR e HOGA1, respectivamente. As mutações em qualquer um desses genes resulta em níveis aumentados de oxalato gerados por processos endógenos no fígado. As formas de hiperoxalúria onde uma causa genética não pode ser estabelecida - hiperoxalúria entérica, dietética e idiopática - são denominadas hiperoxalúrias secundarias. Embora algum nível basal de absorção de oxalato da dieta seja normal, em pacientes com hiperoxalúria entérica a permeabilidade do intestino para o oxalato foi aumentada,
causando maior absorção de oxalato da dieta. Isso pode ser causado por distúrbios da fisiologia do intestino a partir de condições incluindo doença inflamatória intestinal e bypass gástrico. A hiperoxalúria dietética decorre de um consumo excessivo de oxalato ou precursores de oxalato na dieta. Finalmente, para pacientes com hiperoxalúria idiopática uma causa aguda de sua hiperoxalúria não foi identificada.
[004] Existem poucos tratamentos para hiperoxalúria. Para os pacientes com hiperoxalúria primária com uma mutação em AXGT, a piridoxina pode ser prescrita para salvar alguma atividade da enzima mutada. Alternativamente, os pacientes com hiperoxalúria primária podem receber um transplante de fígado para repor as enzimas hepáticas ausentes. Para o restante de pacientes com hiperoxalúria, uma dieta com alto teor de cálcio e baixo teor de oxalato é a intervenção primária, junto com outros tratamentos destinados a corrigir complicações - cálculos renais e insuficiência renal. Entretanto, o oxalato é difícil de evitar, mesmo com dieta cuidadosa, e instruir os pacientes a evitar o oxalato costuma ser insuficiente. Com esses tratamentos limitados disponíveis para mitigar a hiperoxalúria, os pacientes precisam de um tratamento poderoso e estável para remover o oxalato de seus corpos.
[005] Consequentemente, existe uma necessidade contínua por terapias novas e eficazes para tratar e controlar doenças ou transtornos associados com uma quantidade elevada de oxalato, tais como hiperoxalúria.
BREVE SUMÁRIO
[006] A divulgação se refere geralmente a uma bactéria que foi modificada para ter atividade degradante de oxalato aumentada. As bactérias divulgadas são úteis para tratar transtornos associados com uma quantidade elevada de oxalato em um sujeito, e.g., hiperoxalúria.
[007] Por exemplo, em um aspecto, é aqui fornecida uma bactéria comensal que foi modificada para ter atividade degradante de oxalato aumentada em relação a uma bactéria similar ou de outro modo idêntica que não foi modificada. Em certas modalidades, uma bactéria similar ou de outro modo idêntica que não foi modificada não tem atividade degradante de oxalato detectável e/ou não tem genes conhecidos por estarem envolvidos, e.g., diretamente envolvidos no catabolismo de oxalato. Em certas modalidades, nenhum membro que ocorre naturalmente do mesmo gênero que a bactéria tem atividade degradante de oxalato detectável.
[008] Em certas modalidades, uma bactéria considerada é de um gênero selecionado do grupo que consiste em Bacteroides, Alistipes, Faecalibacterium, ParaBacteroides, Prevotella, Roseburia, Ruminococcus, Clostridium, Oscillibacter, Gemmiger, Barnesiella, Dialister, Parasutterella, Phascolarctobacterium, Propionibacterium, Sutterella, Blautia, Paraprevotella, Coprococcus, Odoribacter, Spiroplasma, Anaerostipes e Akkermansia. Por exemplo, uma bactéria considerada pode ser do gênero Bacteroides, i.e., pode ser uma bactéria da espécie Bacteroides.
[009] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma proteína, ou um fragmento funcional ou variante da mesma, selecionada do grupo que consiste em: uma oxalato:formiato antiportadora (OxlT); uma oxalato descarboxilase (OXDC)- EC 4.1.1.2; uma oxalato oxidase (OXO)- EC 1.2.3.4; uma oxalato óxido-redutase (OOR)- EC 1.2.7.10; uma oxalato-CoA ligase/Oxalil-CoA sintetase (OXS)- EC 6.2.1.8; uma formil-CoA:oxalato CoA- transferase (FCOCT)- EC 2.8.3.16; uma acetil-CoA:oxalato CoA-transferase (ACOCT)- EC 2.8.3.19; uma succinil-CoA:oxalato CoA-transferase (SCOCT)- EC
2.8.3.2; uma oxalil-CoA descarboxilase (OXC)- EC 4.1.1.8; uma oxalil-CoA redutase/glioxilato:NADP+ óxido-redutase (OXR)- EC 1.2.1.17; e uma combinação de qualquer uma das proteínas anteriores. É considerado que o um ou mais transgenes podem, e.g., estar em um plasmídeo, cromossomo bacteriano artificial ou serem genomicamente integrados. Quando uma bactéria compreende um ou mais transgenes que codificam múltiplas proteínas, é considerado que os quadros de leitura abertos que codificam duas ou mais das proteínas podem, e.g., estar presentes em um único operon.
[010] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma OXS ou um fragmento funcional ou variante da mesma e uma OXC ou um fragmento funcional ou variante da mesma. Em certas modalidades, uma bactéria compreende ainda um ou mais transgenes que codificam uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma.
[011] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma OXS ou um fragmento funcional ou variante da mesma, uma primeira OXC ou um fragmento funcional ou variante da mesma, uma segunda OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma. Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma primeira OXS ou um fragmento funcional ou variante da mesma, uma segunda OXS ou um fragmento funcional ou variante da mesma, uma OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma. Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma primeira OXS ou um fragmento funcional ou variante da mesma, uma segunda OXS ou um fragmento funcional ou variante da mesma, uma primeira OXC ou um fragmento funcional ou variante da mesma, uma segunda OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma.
[012] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT de O. formigenes; um S. cerevisiae ou uma OXS de A. thaliana; um S. cerevisiae ou uma OXC de O. formigenes; uma FCOCT de E. coli; e/ou uma ACOCT de E. coli. Por exemplo, é considerado que uma bactéria pode compreender: (i) um ou mais transgenes que codificam uma OXS de S. cerevisiae, uma OXC de S. cerevisiae e uma OxIT de O. formigenes; (ii) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae e uma OxIT de O. formigenes; (iii) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OxIT de O. formigenes; (iv) ou um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma OXS de A. thaliana, uma ACOCT de E. coli e uma OxIT de O. formigenes; (v) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OxIT de O. formigenes, uma ACOCT de E. coli e uma OXS de S. cerevisiae; (vi) um ou mais transgenes que codificam uma OxIT de O. formigenes, uma FCOCT de E. coli, uma OXC de S. cerevisiae, uma ACOCT de E. coli, uma OXS de S. cerevisiae e uma OXS de A. thaliana; ou (vii) um ou mais transgenes que codificam uma OxIT de O. formigenes, uma FCOCT de E. coli, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OXS de S. cerevisiae.
[013] Em certas modalidades, uma bactéria considerada compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID NOs: 1 a 31, ou compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 1 a
31.
[014] Por exemplo, uma bactéria considerada pode compreender: (i) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21; (ii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21; (iii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21; (iv) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 14, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21; (v) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16; (vi) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 14; ou (vii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO:
16.
[015] Por exemplo, uma bactéria considerada pode compreender: (i) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163 e SEQ ID NO: 21; (ii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16 e SEQ ID NO: 21; (iii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2 e SEQ ID NO: 21;
(iv) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 14, SEQ ID NO: 2 e SEQ ID NO: 21; (v) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 21, SEQ ID NO: 2 e SEQ ID NO: 16; (vi) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 21, SEQ ID NO: 7, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2, SEQ ID NO: 16 e SEQ ID NO: 14; ou (vii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 21, SEQ ID NO: 7, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2 e SEQ ID NO: 16.
[016] Em certas modalidades, um transgene ou ácido nucleico está operacionalmente ligado a um sítio de ligação ao ribossomo (RBS). RBSs exemplificativos incluem aqueles compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 164 a 230. Por exemplo, uma bactéria considerada pode compreender: (i) um transgene que codifica uma OxIT de O. formigenes operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 212 a 219; (ii) um transgene que codifica uma OXS de S. cerevisiae operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 172 a 179; (iii) um transgene que codifica uma OXS de A. thaliana operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 164 a 171; (iv) um transgene que codifica uma OXC de S. cerevisiae operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 188 a 195 ou 220 a 230; (v) um transgene que codifica uma OXC de O. formigenes operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 180 a 187; (vi) um transgene que codifica uma FCOCT de E. coli operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 204 a 211; e/ou (vii) transgene que codifica uma ACOCT de E. coli operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 196 a 203.
[017] Em certas modalidades, uma bactéria considerada compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID NOs: 96 a 162, ou compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 96 a 162.
[018] Por exemplo, uma bactéria considerada pode compreender: (i) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 104; (ii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 148, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (iii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 144, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (iv) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 147, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 104, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 97, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 131, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 136 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113; (v) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; (vi) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; (vii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 112, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 99, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145; (viii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 154, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 131, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 149; (ix) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 148, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO:
129 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (x) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (xi) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 144, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (xii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; (xiii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; ou (xiv) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 112, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 99, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145.
[019] Por exemplo, uma bactéria considerada pode compreender: (i) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 137, SEQ ID NO: 113, SEQ ID NO: 145, SEQ ID NO: 128 e SEQ ID NO: 104; (ii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 148, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105; (iii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 144, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105; (iv) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 147, SEQ ID NO: 104, SEQ ID NO: 153, SEQ ID NO: 97, SEQ ID NO: 131, SEQ ID NO: 136 e SEQ ID NO: 113; (v) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153,
SEQ ID NO: 129, SEQ ID NO: 105, SEQ ID NO: 113 e SEQ ID NO: 96; (vi) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105 e SEQ ID NO: 96; (vii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 129, SEQ ID NO: 155, SEQ ID NO: 112, SEQ ID NO: 99, SEQ ID NO: 106 e SEQ ID NO: 145; (viii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 154, SEQ ID NO: 131, SEQ ID NO: 98, SEQ ID NO: 115, SEQ ID NO: 106 e SEQ ID NO: 149; (ix) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 137, SEQ ID NO: 113, SEQ ID NO: 148, SEQ ID NO: 129 e SEQ ID NO: 105; (x) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129 e SEQ ID NO: 105; (xi) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 144, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105; (xii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105, SEQ ID NO: 113 e SEQ ID NO: 96; (xiii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105 e SEQ ID NO: 96; ou (xiv) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 129, SEQ ID NO: 155, SEQ ID NO: 112, SEQ ID NO: 99, SEQ ID NO: 106 e SEQ ID NO: 145.
[020] Em certas modalidades, uma bactéria considerada compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID NOs: 82 a 95, ou compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 82 a 95.
[021] Em certas modalidades, um transgene ou ácido nucleico está operacionalmente ligado a pelo menos um promotor constitutivo, e.g., um promotor derivado de fago. Promotores exemplificativos incluem aqueles compreendendo a sequência consenso GTTAA(n)4-7GTTAA(n)34-38TA(n)2TTTG (SEQ ID: 70) ou compreendendo a SEQ ID NO: 68, SEQ ID NO: 69 ou SEQ ID NO: 71.
[022] Em certas modalidades, uma bactéria considerada foi modificada para colonizar o intestino humano com maior abundância, estabilidade, previsibilidade ou facilidade de colonização inicial em relação a uma bactéria similar ou de outro modo idêntica que não foi modificada. Por exemplo, uma bactéria considerada pode ser modificada para aumentar sua capacidade de utilizar um nutriente privilegiado como fonte de carbono. Por exemplo, uma bactéria considerada pode compreender um ou mais transgenes que aumentam sua capacidade de utilizar um nutriente privilegiado como fonte de carbono. Nutrientes privilegiados exemplificativos incluem, e.g., um polissacarídeo marinho, e.g., um porfirano. Uma bactéria divulgada pode, e.g., após a administração a um sujeito humano, resultar em uma abundância maior que 1012, 1011, 1010, 109, 108 ou 107 cfu por grama de conteúdo fecal.
[023] Em outro aspecto, é aqui fornecida uma composição farmacêutica compreendendo uma bactéria divulgada e um excipiente farmaceuticamente aceitável. Em certas modalidades, uma composição farmacêutica considerada é formulada como uma cápsula ou comprimido, e.g., uma cápsula revestida entérica. Em certas modalidades, uma composição farmacêutica considerada compreende ainda um nutriente privilegiado, e.g., um polissacarídeo marinho, e.g., um porfirano.
[024] Em outro aspecto, é aqui fornecido um método de reduzir oxalato em um sujeito em necessidade do mesmo. Um método considerado compreende administrar ao sujeito uma quantidade eficaz de uma bactéria ou composição farmacêutica divulgada. Em certas modalidades, um sujeito tem um transtorno associado com uma quantidade elevada de oxalato, e.g., hiperoxalúria. Em outro aspecto, é aqui fornecido um método de tratar um transtorno associado com uma quantidade elevada de oxalato em um sujeito, e.g., hiperoxalúria. Um método considerado compreende administrar ao sujeito uma quantidade eficaz de uma bactéria ou composição farmacêutica divulgada.
[025] Métodos considerados podem compreender a administração de uma bactéria ou composição farmacêutica divulgada a um sujeito a cada 12 horas, 24 horas, dia, 2 dias, 3 dias, 4 dias, 5 dias, 6 dias, 7 dias, semana, 2 semanas, 3 semanas, 4 semanas, mês, 2 meses, 3 meses, 4 meses, 5 meses ou 6 meses. Em certas modalidades, o tempo entre as administrações consecutivas de uma bactéria ou composição farmacêutica divulgada a um sujeito é maior do que 48 horas.
[026] Métodos considerados podem compreender ainda administrar um nutriente privilegiado ao sujeito, e.g., um polissacarídeo marinho, e.g., um porfirano. Por exemplo, um nutriente privilegiado divulgado pode ser administrado ao sujeito antes de, ao mesmo tempo que ou depois de uma bactéria divulgada.
[027] Em outro aspecto, a invenção fornece um vetor de expressão que codifica uma proteína, ou um fragmento funcional ou variante da mesma, selecionado do grupo que consiste em: uma oxalato:formiato antiportadora (OxlT); uma oxalato descarboxilase (OXDC)- EC 4.1.1.2; uma oxalato oxidase (OXO)- EC 1.2.3.4; uma oxalato óxido-redutase (OOR)- EC 1.2.7.10; uma oxalato-CoA ligase/Oxalil-CoA sintetase (OXS)- EC 6.2.1.8; uma formil-CoA:oxalato CoA-transferase (FCOCT)- EC
2.8.3.16; uma acetil-CoA:oxalato CoA-transferase (ACOCT)- EC 2.8.3.19; uma succinil-CoA:oxalato CoA-transferase (SCOCT)- EC 2.8.3.2; uma oxalil-CoA descarboxilase (OXC)- EC 4.1.1.8; uma oxalil-CoA redutase/glioxilato:NADP+ óxido- redutase (OXR)- EC 1.2.1.17; e uma combinação de qualquer uma das proteínas anteriores. Vetores de expressão exemplificativos incluem, e.g., um plasmídeo e um cromossomo bacteriano artificial. Quando um vetor de expressão compreende um ou mais transgenes que codificam múltiplas proteínas, é considerado que os quadros de leitura abertos que codificam duas ou mais das proteínas podem, e.g., estar presentes em um único operon.
[028] Em certas modalidades, um vetor de expressão considerado compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma OXS ou um fragmento funcional ou variante da mesma e uma OXC ou um fragmento funcional ou variante da mesma. Em certas modalidades, um vetor de expressão compreende ainda um ou mais transgenes que codificam uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma.
[029] Em certas modalidades, um vetor de expressão considerado compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma OXS ou um fragmento funcional ou variante da mesma, uma primeira OXC ou um fragmento funcional ou variante da mesma, uma segunda OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma. Em certas modalidades, um vetor de expressão considerado compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma primeira OXS ou um fragmento funcional ou variante da mesma, uma segunda OXS ou um fragmento funcional ou variante da mesma, uma OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma. Em certas modalidades, um vetor de expressão considerado compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma primeira OXS ou um fragmento funcional ou variante da mesma, uma segunda OXS ou um fragmento funcional ou variante da mesma, uma primeira OXC ou um fragmento funcional ou variante da mesma, uma segunda OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma.
[030] Em certas modalidades, um vetor de expressão considerado compreende um ou mais transgenes que codificam uma OxIT de O. formigenes; um S. cerevisiae ou uma OXS de A. thaliana; um S. cerevisiae ou uma OXC de O. formigenes; uma FCOCT de E. coli; e/ou uma ACOCT de E. coli. Por exemplo, é considerado que um vetor de expressão pode compreender: (i) um ou mais transgenes que codificam uma OXS de S. cerevisiae, uma OXC de S. cerevisiae e uma OxIT de O. formigenes; (ii) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae e uma OxIT de O. formigenes; (iii) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OxIT de O. formigenes; (iv) ou um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma OXS de A. thaliana, uma ACOCT de E. coli e uma OxIT de O. formigenes; (v) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OxIT de O. formigenes, uma ACOCT de E. coli e uma OXS de S. cerevisiae; (vi) um ou mais transgenes que codificam uma OxIT de O. formigenes, uma FCOCT de E. coli, uma OXC de S. cerevisiae, uma ACOCT de E. coli, uma OXS de S. cerevisiae e uma OXS de A. thaliana; ou (vii) um ou mais transgenes que codificam uma OxIT de O. formigenes, uma FCOCT de E. coli, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OXS de S. cerevisiae.
[031] Em certas modalidades, um vetor de expressão considerado compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID
NOs: 1 a 31, ou compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 1 a 31.
[032] Por exemplo, um vetor de expressão considerado pode compreender: (i) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21; (ii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21; (iii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21; (iv) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 14, uma sequência de nucleotídeos tendo pelo menos
80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21; (v) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16; (vi) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 14; ou (vii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO:
16.
[033] Por exemplo, um vetor de expressão considerado pode compreender: (i) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163 e SEQ ID NO: 21; (ii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7,
SEQ ID NO: 25, SEQ ID NO: 16 e SEQ ID NO: 21; (iii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2 e SEQ ID NO: 21; (iv) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 14, SEQ ID NO: 2 e SEQ ID NO: 21; (v) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 21, SEQ ID NO: 2 e SEQ ID NO: 16; (vi) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 21, SEQ ID NO: 7, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2, SEQ ID NO: 16 e SEQ ID NO: 14; ou (vii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 21, SEQ ID NO: 7, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2 e SEQ ID NO: 16.
[034] Em certas modalidades, um transgene ou ácido nucleico está operacionalmente ligado a um sítio de ligação ao ribossomo (RBS). RBSs exemplificativos incluem aqueles compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 164 a 230. Por exemplo, um vetor de expressão considerado pode compreender: (i) um transgene que codifica uma OxIT de O. formigenes operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 212 a 219; (ii) um transgene que codifica uma OXS de S. cerevisiae operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 172 a 179; (iii) um transgene que codifica uma OXS de A. thaliana operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 164 a 171; (iv) um transgene que codifica uma OXC de S. cerevisiae operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 188 a 195 ou 220 a 230; (v) um transgene que codifica uma OXC de O. formigenes operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 180 a 187; (vi) um transgene que codifica uma FCOCT de E. coli operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 204 a 211; e/ou (vii) transgene que codifica uma ACOCT de E. coli operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 196 a 203.
[035] Em certas modalidades, um vetor de expressão considerado compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID NOs: 96 a 162, ou compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 96 a 162.
[036] Por exemplo, um vetor de expressão considerado pode compreender: (i) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 104; (ii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 148, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (iii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 144, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (iv) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 147, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 104, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 97, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 131, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 136 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113; (v) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO:
96; (vi) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; (vii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 112, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 99, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145; (viii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 154, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 131, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 149; (ix) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 148, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (x) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (xi) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 144, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (xii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; (xiii) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; ou (xiv) um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 112, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 99, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145.
[037] Por exemplo, um vetor de expressão considerado pode compreender: (i) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 137, SEQ ID NO: 113, SEQ ID NO: 145, SEQ ID NO: 128 e SEQ ID NO: 104; (ii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 148, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105; (iii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 144, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105; (iv) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 147, SEQ ID NO: 104, SEQ ID NO: 153, SEQ ID NO: 97, SEQ ID NO: 131, SEQ ID NO: 136 e SEQ ID NO: 113; (v) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105, SEQ ID NO: 113 e SEQ ID NO: 96; (vi) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105 e SEQ ID NO: 96; (vii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 129, SEQ ID NO: 155, SEQ ID NO: 112, SEQ ID NO: 99, SEQ ID NO: 106 e SEQ ID NO: 145; (viii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 154, SEQ ID NO: 131, SEQ ID NO: 98, SEQ ID NO: 115, SEQ ID NO: 106 e SEQ ID NO: 149; (ix) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 137, SEQ ID NO: 113, SEQ ID NO: 148, SEQ ID NO: 129 e SEQ ID NO: 105; (x) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129 e SEQ ID NO: 105; (xi) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 144, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105; (xii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105, SEQ ID NO: 113 e SEQ ID NO: 96; (xiii) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105 e SEQ ID NO: 96; ou (xiv) um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 129, SEQ ID NO: 155, SEQ ID NO: 112, SEQ ID NO: 99, SEQ ID NO: 106 e SEQ ID NO: 145.
[038] Em certas modalidades, um vetor de expressão considerado compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID NOs: 82 a 95, ou compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 82 a 95.
[039] Em certas modalidades, um transgene ou ácido nucleico está operacionalmente ligado a pelo menos um promotor constitutivo, e.g., um promotor derivado de fago. Promotores exemplificativos incluem aqueles compreendendo a sequência consenso GTTAA(n)4-7GTTAA(n)34-38TA(n)2TTTG (SEQ ID: 70), ou compreendendo a SEQ ID NO: 68, SEQ ID NO: 69 ou SEQ ID NO: 71.
[040] Esses e outros aspectos e características da divulgação são descritos na seguinte descrição detalhada e reivindicações.
DESCRIÇÃO DOS DESENHOS
[041] A divulgação pode ser mais completamente entendida com referência aos seguintes desenhos.
[042] A FIGURA 1 é uma representação esquemática das vias para degradação de oxalato. O oxalato é importado em células bacterianas usando a oxalato:formiato antiportadora OxIT e então convertido em formiato, glioxilato ou CO2 por meio de uma variedade de vias diferentes. A via mais produtiva usa OXS, FCOCT, ACOCT ou SCOCT para conjugar oxalato com CoA para criar oxalil-CoA, e então OXC para decarboxilar oxalil-CoA em formil-CoA. FCOCT é então usada para liberar formiato e reiniciar o ciclo.
[043] A FIGURA 2 é um gráfico de setores que mostra a abundância bacteriana no intestino humano médio com base em sequenciamento de DNA ribossômico 16S. Bacteroides é o gênero mais abundante com 46 %.
[044] A FIGURA 3 descreve uma representação esquemática de cinco plasmídeos diferentes (SEQ ID NOs: 63 a 67) que podem ser conjugados em Bacteroides para produzir cepas de Bacteroides degradantes de oxalato.
[045] A FIGURA 4 é um gráfico de barras que representa a quantidade de oxalato consumido por Bacteroides vulgatus ATCC 8482 sem transgenes (tipo selvagem) ou com integração genômica dos seguintes plasmídeos: pZR748 (SEQ ID NO: 63), pZR753 (SEQ ID NO: 64), pZR753 + pZR761 (SEQ ID NOs: 64, 65) ou pZR856 + pZR878 (SEQ ID NOs: 66, 67). Culturas durante a noite de cada cepa de Bacteroides foram diluídas 1:10 em meios líquidos BHIS frescos suplementados com 10 ou 30 mM de oxalato e as taxas de consumo de oxalato foram monitoradas durante 48 horas.
[046] A FIGURA 5 descreve uma representação esquemática de plasmídeo pWD035 (SEQ ID NO: 73), um cromossomo bacteriano artificial com uma origem de transferência conjugativa e um loco de utilização de polissacarídeo porfirano (PUL).
[047] A FIGURA 6 descreve um ensaio de crescimento in vitro de células de Bacteroides vulgatus que receberam um PUL porfirano funcional (pWD035, SEQ ID NO: 73) em comparação com células de B. vulgatus tipo selvagem. Culturas saturadas foram diluídas 50X em meios mínimos suplementados com 0,2 % de porfirano e cultivadas anaerobicamente por 14 horas a 37 °C. A densidade óptica das culturas foi monitorada do começo ao fim. Apenas as células que continham o PUL porfirano foram capazes de crescer em porfirano.
[048] A FIGURA 7 descreve três plasmídeos diferentes (SEQ ID NOs: 75 a 77) que abrigam genes de consumo de oxalato. Cada plasmídeo foi conjugado na cepa NB075 utilizando porfirano para produzir cepas que podem consumir um nutriente privilegiado, porfirano e uma toxina derivada do intestino, oxalato.
[049] A FIGURA 8 é um gráfico de linha que representa a taxa de consumo de oxalato para as cepas projetadas indicadas de Bacteroides após a adição das cepas a 1010 cfu/ml aos meios BHIS frescos contendo 4mM de oxalato.
[050] A FIGURA 9 é um gráfico de linha que representa a taxa de consumo de oxalato para as cepas projetadas indicadas de Bacteroides após a adição das cepas a 1010 cfu/ml aos conteúdos cecais de ratos Sprague-Dawley contendo 4mM de oxalato.
[051] A FIGURA 10 é um gráfico de barras que representa os níveis de oxalato na urina em um modelo de rato de hiperoxalúria secundária após o tratamento com a cepa indicada. Em relação a uma cepa controle que não consome oxalato (NB075), a cepa que consome oxalato sWW152 produz uma redução de 32 % no oxalato na urina 4 a 6 dias após a administração da cepa (p=0,005). As barras de erro representam intervalos de confiança de 95 %.
[052] A FIGURA 11 é um gráfico de linha que representa os níveis de oxalato na urina em um modelo de rato de hiperoxalúria secundária após o tratamento com a cepa indicada. Em relação a uma cepa controle que não consome oxalato (NB124), a cepa que consome oxalato sWW554 produz uma redução de 46 % no oxalato na urina 4 a 6 dias após a administração da cepa (p=0,0003). As regiões sombreadas representam intervalos de confiança de 95 %.
[053] A FIGURA 12 é um gráfico de barras que representa a abundância fecal de cada cepa do dia 12 do experimento resumido na FIGURA 11.
[054] A FIGURA 13A é um gráfico de barras que representa a abundância de cepa (medida por unidades formadoras de colônia (CFU) por grama de fezes) em um modelo de rato de hiperoxalúria secundária após o tratamento com a cepa indicada e a quantidade indicada (% em peso) de porfirano administrado na água potável. A FIGURA 13B é um gráfico de barras que representa os níveis de oxalato na urina em um modelo de rato de hiperoxalúria secundária após o tratamento com a cepa indicada e a quantidade indicada (% em peso) de porfirano administrado na água potável. As barras representam a média, os pontos representam animais individuais e as barras de erro representam intervalos de confiança de 95 %.
[055] A FIGURA 14 é um gráfico de barras que representa os níveis de oxalato na urina em ratos com hiperoxalúria induzida através de cirurgia de Bypass gástrico em Y-de-Roux (RYGB) após o tratamento com a cepa indicada. As barras representam a média final de cinco dias consecutivos de oxalato diário total na urina dos ratos em cada grupo. As barras de erro representam os intervalos de confiança de 95 % (n = 5 ratos sham, n = 8 para todos os outros grupos).
[056] A FIGURA 15A descreve os resultados da triagem de sítios de ligação ao ribossomo (RBSs). Cada ponto representa a expressão (conforme medida por valores de luminescência log10) para cada gene indicado com um diferente RBS projetado, como descrito no Exemplo 8. A FIGURA 15B descreve a expressão (conforme medida por luminescência) do gene indicado com o RBS indicado. As barras de erro representam os intervalos de confiança de 95 %.
[057] A FIGURA 16 é um gráfico de barras que representa a degradação de oxalato in vitro pelas cepas indicadas. As barras de erro representam os intervalos de confiança de 95 %.
DESCRIÇÃO DETALHADA
[058] O intestino humano, especificamente o cólon, é conhecido por ser permeável ao oxalato. Ele funciona como o sítio primário de absorção de oxalato dietético e é considerado que a secreção de oxalato para fora da corrente sanguínea e no lúmen intestinal fornece uma via secundária para a eliminação de oxalato do corpo. Assim, o cólon é um local atrativo para um sistema para degradar oxalato, prevenindo a absorção de oxalato dietético e a reabsorção de oxalato endógeno secretado.
[059] A divulgação se refere geralmente a bactérias que foram modificadas para ter atividade degradante de oxalato aumentada. Por exemplo, em um aspecto, é aqui fornecida uma bactéria comensal que foi modificada para ter atividade degradante de oxalato aumentada em relação a uma bactéria similar ou de outro modo idêntica que não foi modificada. É considerado que as bactérias divulgadas podem, após a administração a um sujeito, quebrar o oxalato no sujeito, e.g., no intestino grosso do sujeito e, portanto, serem úteis para tratar um transtorno associado com uma quantidade elevada de oxalato no sujeito, e.g., hiperoxalúria. Consequentemente, a divulgação se refere ainda às composições farmacêuticas ou unidades e métodos de uso das bactérias divulgadas para tratar transtornos associados com uma quantidade elevada de oxalato, e.g., hiperoxalúria.
[060] Uma bactéria modificada considerada pode adicionalmente ter a capacidade de utilizar uma fonte de carbono, tal como o polissacarídeo marinho porfirina, que outras bactérias no intestino de um sujeito a ser tratado são amplamente incapazes de utilizar. Como um resultado, a proliferação, abundância ou estabilidade da bactéria modificada no intestino do sujeito pode ser mantida fornecendo-lhe a fonte de carbono. I. Bactéria Modificada
[061] A divulgação se refere geralmente às bactérias que foram modificadas para ter atividade degradante de oxalato aumentada em relação a uma bactéria similar ou de outro modo idêntica que não foi modificada. Por exemplo, uma bactéria considerada pode ser modificada para expressar um ou mais transgenes que aumentam atividade de degradação de oxalato. O termo “degradação de oxalato” é usado permutavelmente com o termo “consumo de oxalato” aqui. As vias para a degradação de oxalato, incluindo genes relacionados à degradação de oxalato, são representadas na FIGURA 1. É considerado que o um ou mais transgenes podem, e.g., estar em um plasmídeo, cromossomo bacteriano artificial ou serem genomicamente integrados. Quando uma bactéria compreende um ou mais transgenes que codificam múltiplas proteínas, é considerado que os quadros de leitura abertos que codificam duas ou mais das proteínas podem, e.g., estar presentes em um único operon.
[062] Genes exemplificativos relacionados à degradação de oxalato, cuja expressão em uma bactéria pode aumentar a atividade de degradação de oxalato,
incluem aqueles que codificam: uma oxalato:formiato antiportadora (OxlT); uma oxalato descarboxilase (OXDC)- EC 4.1.1.2; uma oxalato oxidase (OXO)- EC 1.2.3.4; uma oxalato óxido-redutase (OOR)- EC 1.2.7.10; uma oxalato-CoA ligase/Oxalil-CoA sintetase (OXS)- EC 6.2.1.8; uma formil-CoA:oxalato CoA-transferase (FCOCT)- EC
2.8.3.16; uma acetil-CoA:oxalato CoA-transferase (ACOCT)- EC 2.8.3.19; uma succinil-CoA:oxalato CoA-transferase (SCOCT)- EC 2.8.3.2; uma oxalil-CoA descarboxilase (OXC)- EC 4.1.1.8; e uma oxalil-CoA redutase/glioxilato:NADP+ óxido- redutase (OXR)- EC 1.2.1.17.
[063] Para que uma bactéria degrade oxalato, a expressão de um transportador de oxalato pode ser necessária, tipicamente uma oxalato:formiato antiportadora. Transportadores de oxalato exemplificativos incluem OxlT de Oxalobacter formigenes ou qualquer um de seus homólogos de outra espécie incluindo Cupriavidus oxalaticus e Bifidobacterium animalis subsp. lactis. Esses antiportadores trazem oxalato na célula bacteriana do ambiente intestinal e, em troca, expelem uma molécula de resíduo de formiato para cada molécula de oxalato introduzida. Uma vez que o oxalato entra na célula, pode ser diretamente degradado usando a oxalato descarboxilase (OXDC) - EC 4.1.1.2, oxalato oxidase (OXO) - EC
1.2.3.4 ou oxalato óxido-redutase (OOR) - EC 1.2.7.10.
[064] Alternativamente, o oxalato pode ser conjugado com coenzima A para criar oxalil-CoA usando oxalato-CoA ligase (OXS) - EC 6.2.1.8, formil-CoA:oxalato CoA-transferase (FCOCT) - EC 2.8.3.16, acetil-CoA:oxalato CoA-transferase (ACOCT) - EC 2.8.3.19 ou succinil-CoA:oxalato CoA-transferase (SCOCT) - EC
2.8.3.2. FCOCT usa formil-CoA como um doador de CoA e, como formil-CoA é gerada como um produto residual durante a degradação de oxalil-CoA, resulta em um ciclo eficiente. Também pode ser necessário iniciar o ciclo usando material de partida mais abundante, tal como ATP, acetil-CoA ou succinil-CoA, usados por OXS, ACOCT e SCOCT respectivamente.
[065] Oxalil-CoA é quebrada por oxalil-CoA descarboxilase (OXC) - EC
4.1.1.8. Essa, por sua vez, gera formil-CoA, que é degradada por FCOCT para liberar formiato. Então, o formiato pode ser degradado pela formiato desidrogenase (FDH) - EC 1.2.1.2 para gerar CO2. Uma opção adicional para o descarte de oxalil-CoA é a redução no glioxilato para a construção de biomassa adicional via oxalil-CoA redutase (OXR)/Glioxilato:NADP+ óxido-redutase - EC 1.2.1.17.
[066] Em certas modalidades de uma bactéria modificada considerada, o oxalato é importado na bactéria usando uma antiportadora OxlT e alimentado em um ciclo contínuo de conversão de oxalato em formiato usando FCOCT e OXC. Outro suporte para este ciclo pode ser fornecido pela expressão aumentada de múltiplas cópias de enzimas OXC. Adicionalmente, o pool de ciclos intermediários, oxalil-CoA e formil-CoA, pode ser criado e sustentado pela expressão de OXS, ACOCT ou SCOCT. Assim, em certas modalidades, uma célula bacteriana considerada tem alta expressão de multicópias de OXC e FCOCT, alta expressão de OXS, ACOCT e/ou SCOCT, e expressão moderada de OxlT. Em certas modalidades, um fragmento N-terminal de uma oxalato:formiato antiportadora foi trocado por um fragmento N-terminal de uma proteína transportadora nativa.
[067] Consequentemente, em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma OXS ou um fragmento funcional ou variante da mesma e uma OXC ou um fragmento funcional ou variante da mesma. Em certas modalidades, uma bactéria compreende ainda um ou mais transgenes que codificam uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma.
[068] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma OXS ou um fragmento funcional ou variante da mesma, uma primeira
OXC ou um fragmento funcional ou variante da mesma, uma segunda OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma. Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma primeira OXS ou um fragmento funcional ou variante da mesma, uma segunda OXS ou um fragmento funcional ou variante da mesma, uma OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma. Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma primeira OXS ou um fragmento funcional ou variante da mesma, uma segunda OXS ou um fragmento funcional ou variante da mesma, uma primeira OXC ou um fragmento funcional ou variante da mesma, uma segunda OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma e/ou uma ACOCT ou um fragmento funcional ou variante da mesma.
[069] Como aqui usado, o termo “fragmento funcional” de uma entidade biológica (e.g., um gene, proteína (e.g. ,OxIT, OXS, OXC ou FCOCT), promotor ou sítio de ligação ao ribossomo) se refere a um fragmento da entidade biológica de comprimento completo que mantém, por exemplo, pelo menos 10 %, pelo menos 20 %, pelo menos 30 %, pelo menos 40 %, pelo menos 50 %, pelo menos 60 %, pelo menos 70 %, pelo menos 80 %, pelo menos 90 % ou 100 % da atividade biológica da entidade biológica de ocorrência natural de comprimento completo correspondente.
[070] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT de B. animalis subsp. lactis, e.g., uma OxIT de B. animalis subsp. lactis DSM 10140, uma OxIT de C. oxalaticus, e.g., uma OxIT de C. oxalaticus ATCC 11883, uma OxIT de E. coli, e.g., uma OxIT de E. coli MG1655, uma OxIT de O. formigenes, e.g., uma OxIT de O. formigenes DSM 4420 ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores. Por exemplo, em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OxIT de O. formigenes, e.g., uma OxIT de O. formigenes DSM 4420, ou uma proteína tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com uma OxIT de O. formigenes.
[071] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OXS de S. cerevisiae, e.g., uma OXS de S. cerevisiae S288C, uma OXS de M. extorquens, e.g., uma OXS de M. extorquens AM1/DSM 1338, uma OXS de A. thaliana, e.g., uma OXS de A. thaliana col-1, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores. Por exemplo, em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OXS de S. cerevisiae, e.g., uma OXS de S. cerevisiae S288C, ou uma proteína tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com uma OXS de S. cerevisiae. Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OXS de A. thaliana, e.g., uma OXS de A. thaliana col-1, ou uma proteína tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com uma OXS de A. thaliana.
[072] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OXC de B. animalis subsp. lactis, e.g., uma OXC de B. animalis subsp. lactis DSM 10140, uma OXC de C. oxalaticus, e.g., uma OXC de C. oxalaticus ATCC 11883, uma OXC de E. coli, e.g., uma OXC de E. coli MG1655, uma OXC de S. cerevisiae, e.g., uma OXC de S. cerevisiae S288C, uma OXC de O.
formigenes, e.g., uma OXC de O. formigenes DSM 4420, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores. Por exemplo, em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma OXC de S. cerevisiae, e.g., uma OXC de S. cerevisiae S288C, ou uma proteína tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com uma OXC de S. cerevisiae. Em certas modalidades uma bactéria considerada compreende um ou mais transgenes que codificam uma OXC de O. formigenes, e.g., uma OXC de O. formigenes DSM 4420, ou uma proteína tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com uma OXC de O. formigenes.
[073] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma FCOCT de B. animalis subsp. lactis, e.g., uma FCOCT de B. animalis subsp. lactis DSM 10140, uma FCOCT de C. oxalaticus, e.g., uma FCOCT de C. oxalaticus ATCC 11883, uma FCOCT de E. coli, e.g., uma FCOCT de E. coli MG1655, uma FCOCT de O. formigenes, e.g., uma FCOCT de O. formigenes DSM 4420, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores. Por exemplo, em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma FCOCT de E. coli, e.g., uma FCOCT de E. coli MG1655, ou uma proteína tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com uma FCOCT de E. coli.
[074] Em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma ACOCT de A. aceti, e.g., uma ACOCT de A. aceti cepa 1023, uma ACOCT de E. coli, e.g., uma ACOCT de E. coli MG1655, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores. Por exemplo, em certas modalidades, uma bactéria considerada compreende um ou mais transgenes que codificam uma ACOCT de E. coli, e.g., uma ACOCT de E. coli MG1655, ou uma proteína tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com uma ACOCT de E. coli.
[075] Por exemplo, é considerado que uma bactéria pode compreender: (i) um ou mais transgenes que codificam uma OXS de S. cerevisiae, uma OXC de S. cerevisiae e uma OxIT de O. formigenes, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores; (ii) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae e uma OxIT de O. formigenes, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores; (iii) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OxIT de O. formigenes, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores; (iv) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma OXS de A. thaliana, uma ACOCT de E. coli e uma OxIT de O. formigenes, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores; (v) um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OxIT de O. formigenes, uma ACOCT de E. coli e uma OXS de S. cerevisiae, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores; (vi) um ou mais transgenes que codificam uma OxIT de O. formigenes, uma FCOCT de E. coli, uma OXC de S. cerevisiae, uma ACOCT de E. coli, uma OXS de S. cerevisiae e uma OXS de A. thaliana, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores; ou (vii) um ou mais transgenes que codificam uma OxIT de O. formigenes, uma FCOCT de E. coli, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OXS de S. cerevisiae, ou um fragmento funcional ou variante de qualquer uma das proteínas anteriores.
[076] Sequências de codificação de ACOCT exemplificativas são representadas nas SEQ ID NOs: 1 a 2, sequências de codificação de FDH exemplificativas são representadas nas SEQ ID NOs: 3 a 4, sequências de codificação de FCOCT exemplificativas são representadas nas SEQ ID NOs: 4 a 8, uma sequência de codificação de OXDC exemplificativa é representada na SEQ ID NO: 9, uma sequência de codificação de OXO exemplificativa é representada na SEQ ID NO: 10, sequências de codificação de OOR exemplificativas são representadas nas SEQ ID NOs: 11 a 13, sequências de codificação de OXS exemplificativas são representadas nas SEQ ID NOs: 14 a 16, sequências de codificação de OxIT exemplificativas são representadas nas SEQ ID NOs: 17 a 21, sequências de codificação de OXC exemplificativas são representadas nas SEQ ID NOs: 22 a 26 e 163, uma sequência de codificação de OXR exemplificativa é representada na SEQ ID NO: 27 e sequências de codificação de SCOCT exemplificativas são representadas nas SEQ ID NOs: 28 a 31.
[077] Consequentemente, em certas modalidades, uma bactéria foi modificada para compreender um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 1 a 31, ou um fragmento funcional ou sua variante. Em certas modalidades, uma bactéria foi modificada para compreender um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 1 a 31, ou um fragmento funcional ou sua variante.
[078] Por exemplo, é considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163 e SEQ ID NO: 21, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO:
16, SEQ ID NO: 26 ou SEQ ID NO: 163 e SEQ ID NO: 21. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16 e SEQ ID NO: 21, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16 e SEQ ID NO: 21. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2 e SEQ ID NO: 21, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2 e SEQ ID NO: 21. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 14, SEQ ID NO: 2 e SEQ ID NO: 21, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 16, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 14, SEQ ID NO: 2 e SEQ ID NO: 21. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 21, SEQ ID NO: 2 e SEQ ID NO: 16, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 7, SEQ ID NO: 25, SEQ ID NO: 21, SEQ ID NO: 2 e SEQ ID NO: 16. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 21, SEQ ID NO: 7,
SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2, SEQ ID NO: 16 e SEQ ID NO: 14, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 21, SEQ ID NO: 7, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2, SEQ ID NO: 16 e SEQ ID NO: 14. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 21, SEQ ID NO: 7, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2 e SEQ ID NO: 16, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 21, SEQ ID NO: 7, SEQ ID NO: 26 ou SEQ ID NO: 163, SEQ ID NO: 2 e SEQ ID NO: 16.
[079] Em certas modalidades, uma bactéria foi modificada para compreender um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos que codifica uma sequência de aminoácidos de qualquer uma das SEQ ID NOs: 32 a 62, ou um fragmento funcional ou sua variante. Em certas modalidades, uma bactéria foi modificada para compreender um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos que codifica uma sequência de aminoácidos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 32 a 62, ou um fragmento funcional ou sua variante.
[080] Por exemplo, é considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo uma ou mais sequências de nucleotídeos que codificam as sequências de aminoácidos da SEQ ID NO: 47, SEQ ID NO: 57 e SEQ ID NO: 52, ou sequências de aminoácidos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 47, SEQ ID NO: 57 e SEQ ID NO: 52. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo uma ou mais sequências de nucleotídeos que codificam as sequências de aminoácidos da SEQ ID NO: 38, SEQ ID NO: 56, SEQ ID NO: 47 e SEQ ID NO: 52, ou sequências de aminoácidos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 38, SEQ ID NO: 56, SEQ ID NO: 47 e SEQ ID NO: 52. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo uma ou mais sequências de nucleotídeos que codificam as sequências de aminoácidos da SEQ ID NO: 38, SEQ ID NO: 56, SEQ ID NO: 47, SEQ ID NO: 57, SEQ ID NO: 33 e SEQ ID NO: 52, ou sequências de aminoácidos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 38, SEQ ID NO: 56, SEQ ID NO: 47, SEQ ID NO: 57, SEQ ID NO: 33 e SEQ ID NO: 52. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo uma ou mais sequências de nucleotídeos que codificam as sequências de aminoácidos da SEQ ID NO: 38, SEQ ID NO: 56, SEQ ID NO: 47, SEQ ID NO: 57, SEQ ID NO: 45, SEQ ID NO: 33 e SEQ ID NO: 52, ou sequências de aminoácidos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 38, SEQ ID NO: 56, SEQ ID NO: 47, SEQ ID NO: 57, SEQ ID NO: 45, SEQ ID NO: 33 e SEQ ID NO: 52. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo uma ou mais sequências de nucleotídeos que codificam as sequências de aminoácidos da SEQ ID NO: 38, SEQ ID NO: 56, SEQ ID NO: 52, SEQ ID NO: 33 e SEQ ID NO: 47, ou sequências de aminoácidos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 38, SEQ ID NO: 56, SEQ ID NO: 52, SEQ ID NO: 33 e SEQ ID NO: 47. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo uma ou mais sequências de nucleotídeos que codificam as sequências de aminoácidos da SEQ ID NO: 52, SEQ ID NO: 38, SEQ ID NO: 57, SEQ ID NO: 33, SEQ ID NO: 47 e SEQ ID NO: 45, ou amino ácido tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 52, SEQ ID NO: 38, SEQ ID NO: 57, SEQ ID NO: 33, SEQ ID NO: 47 e SEQ ID NO: 45. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo uma ou mais sequências de nucleotídeos que codificam as sequências de aminoácidos da SEQ ID NO: 52, SEQ ID NO: 38, SEQ ID NO: 57, SEQ ID NO: 33 e SEQ ID NO: 47, ou sequências de aminoácidos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 52, SEQ ID NO: 38, SEQ ID NO: 57 SEQ ID NO: 33 e SEQ ID NO:
47.
[081] A identidade de sequência pode ser determinada de várias maneiras que estão dentro da habilidade na técnica, e.g., usando software de computador publicamente disponível, tal como software BLAST, BLAST-2, ALIGN ou Megalign (DNASTAR). As análises de BLAST (Basic Local Alignment Search Tool) usando o algoritmo utilizado pelos programas blastp, blastn, blastx, tblastn e tblastx (Karlin et al., (1990) Proc. Natl. Acad. Sci. USA 87:2264-2268; Altschul, (1993) J. Mol. Evol. 36, 290-300; Altschul et al., (1997) Nucleic Acids Res. 25:3389-3402, incorporado por referência) são adaptadas para pesquisa de similaridade de sequência. Para um debate de problemas básicos na pesquisa de bancos de dados de sequências ver Altschul et al., (1994) Nature Genetics 6:119-129, que é completamente incorporado por referência. Os especialistas na técnica podem determinar parâmetros apropriados para medir o alinhamento, incluindo quaisquer algoritmos necessários para obter o alinhamento máximo ao longo de todo o comprimento das sequências sendo comparadas. Os parâmetros de pesquisa para histograma, descrições, alinhamentos, espera (i.e., o limiar de significância estatística para relatar correspondências contra sequências do banco de dados), corte, matriz e filtro estão nas configurações padrão. A matriz de pontuação padrão usada pelo blastp, blastx, tblastn e tblastx é a matriz BLOSUM62 (Henikoff et al., (1992) Proc. Natl. Acad. Sci. USA 89:10915-10919, completamente incorporado por referência). Quatro parâmetros de blastn podem ser ajustados como se segue: Q=10 (penalidade de criação de gap); R=10 (penalidade de extensão de gap); wink=1 (gera ocorrência de palavras a cada posição wink.sup.th ao longo da consulta); e gapw=16 (define a largura da janela dentro da qual os alinhamentos espaçados são gerados). Os ajustes de parâmetro de Blastp equivalentes podem ser Q=9; R=2; wink=1; e gapw=32. As pesquisas também podem ser conduzidas usando o parâmetro da Opção Avançada de BLAST do NCBI (National Center for Biotechnology Information) (e.g.: -G, Custo para abrir gap [Número inteiro]: padrão = 5 para nucleotídeos/11 para proteínas; -E, Custo para estender gap [Número inteiro]: padrão = 2 para nucleotídeos/1 para proteínas; -q, Penalidade para correspondência de nucleotídeos [Número inteiro]: padrão = -3; -r, recompensa para correspondência de nucleotídeos [Número inteiro]: padrão = 1; -e, valor de espera [Real]: padrão = 10; -W, tamanho da palavra [Número inteiro]: padrão = 11 para nucleotídeos/28 para megablastos/3 para proteínas; -y, Queda (X) para extensões de blastos em bits: padrão = 20 para blastn/7 para outros; -X, X valor de queda para alinhamento espaçado (em bits): padrão = 15 para todos os programas, não aplicável ao blastn; e -Z, valor X final de queda para alinhamento espaçado (em bits): 50 para blastn, 25 para outros). ClustalW para alinhamentos de proteínas em pares também pode ser usado (parâmetros padrão podem incluir, e.g., matriz Blosum62 e Penalidade de Abertura de Gap = 10 e Penalidade de Extensão de Gap = 0,1). Uma comparação Bestfit entre sequências, disponível no pacote GCG versão 10.0, usa parâmetros de DNA GAP=50 (penalidade de criação de gap) e LEN=3 (penalidade de extensão de gap) e os ajustes equivalentes em comparações de proteínas são GAP=8 e LEN=2.
[082] Uma bactéria modificada considerada, por exemplo, para o uso em uma composição farmacêutica ou método divulgado, inclui uma bactéria do gênero Bacteroides, Alistipes, Faecalibacterium, ParaBacteroides, Prevotella, Roseburia, Ruminococcus, Clostridium, Oscillibacter, Gemmiger, Barnesiella, Dialister, Parasutterella, Phascolarctobacterium, Propionibacterium, Sutterella, Blautia, Paraprevotella, Coprococcus, Odoribacter, Spiroplasma, Anaerostipes ou Akkermansia. Uma bactéria considerada, por exemplo, para o uso em uma composição farmacêutica ou método divulgado, pode ser do gênero Bacteroides, i.e., pode ser uma bactéria da espécie Bacteroides.
[083] Espécies de Bacteroides exemplificativas incluem B. acidifaciens, B. barnesiaes, B. caccae, B. caecicola, B. caecigallinarum, B. cellulosilyticus, B. cellulosolvens, B. clarus, B. coagulans, B. coprocola, B. coprophilus, B. coprosuis, B. distasonis, B. dorei, B. eggerthii, B. gracilis, B. faecichinchillae, B. faecis, B. finegoldii, B. fluxus, B. fragilis, B. galacturonicus, B. gallinaceum, B. gallinarum, B. goldsteinii, B. graminisolvens, B. helcogene, B. intestinalis, B. luti, B. massiliensis, B. melaninogenicus, B. nordii, B. oleiciplenus, B. oris, B. ovatus, B. paurosaccharolyticus, B. pectinophilus, B. plebeius, B. polipragmatus, B. propionicifaciens, B. putredinis, B. pyogenes, B. reticulotermitis, B. rodentium, B. salanitronis, B. salyersiae, B. sartorii, B. sediment B. stercoris, B. suis, B. tectus, B. thetaiotaomicron, B. uniformis, B. vulgatus, B. xylanisolvens e B. xylanolyticusxylanolyticus.
[084] Como aqui usado, o termo “espécie” se refere a uma entidade taxonômica como convencionalmente definida pela sequência genômica e características fenotípicas. Uma “cepa” é um exemplo particular de uma espécie que foi isolada e purificada de acordo com técnicas microbiológicas convencionais. A presente divulgação abrange derivados das cepas bacterianas divulgadas. O termo “derivado” inclui cepas filhas (progênie) ou cepas cultivadas (sub-clonadas) a partir da original, mas modificadas de alguma maneira (incluindo no nível genético), sem alterar negativamente uma atividade biológica da cepa.
[085] Em certas modalidades, uma bactéria modificada considerada é de um gênero que constitui mais de 0,1 %, 0,5 %, 1 %, 5 %, 10 %, 20 %, 30 % ou 40 % do total de micróbios cultiváveis nas fezes de um sujeito a ser tratado, ou nas fezes de um humano médio. Em certas modalidades, uma bactéria modificada considerada é de um gênero que é detectado em um nível maior que 1012, 1011, 1010, 109, 108, 107 unidades formadoras de colônia por grama de fezes de um sujeito a ser tratado, ou por grama de fezes de um humano médio. Em certas modalidades, uma bactéria modificada considerada é de um gênero que constitui mais de 0,1 %, 0,5 %, 1 %, 5 %, 10 %, 20 %, 30 % ou 40 % do microbioma do intestino de um sujeito a ser tratado, ou do microbioma do intestino de um humano médio. Composição do microbioma do intestino ou fezes humanas pode ser avaliada por qualquer técnica conhecida na técnica, incluindo sequenciamento ribossomal 16S. A FIGURA 2 mostra a abundância bacteriana no intestino humano médio com base no sequenciamento de DNA ribossômico 16S. Bacteroides é o gênero mais naturalmente abundante no intestino humano.
[086] rRNA, rDNA 16S, rRNA 16S, 16S, 18S, 18S rRNA e rDNA 18S se referem aos ácidos nucleicos que são componentes de, ou codificam para, os componentes do ribossomo. Existem duas subunidades no ribossomo denominadas subunidade pequena (SSU) e subunidade grande (LSU). Os genes de rDNA e suas sequências de RNA complementares são amplamente usados para a determinação das relações evolutivas entre os organismos, pois são variáveis, embora suficientemente conservados para permitir comparações moleculares entre os organismos.
[087] A sequência rDNA 16S (aproximadamente 1542 nucleotídeos em comprimento) da 30S SSU pode ser usada, em certas modalidades, para atribuições taxonômicas com base molecular de procarióticos e a sequência rDNA 18S (aproximadamente 1869 nucleotídeos em comprimento) de SSU 40S pode ser usada para eucarióticos. Por exemplo, as sequências 16S podem ser usadas para reconstrução filogenética, pois geralmente são altamente conservadas, mas contêm regiões hipervariáveis específicas que abrigam diversidade suficiente de nucleotídeos para diferenciar gêneros e espécie da maioria das bactérias. Embora os dados da sequência rDNA 16S tenham sido usados para fornecer a classificação taxonômica, cepas bacterianas intimamente relacionadas que são classificadas dentro do mesmo gênero e espécie podem exibir fenótipos biológicos distintos.
[088] A identidade das espécies ou cepas bacterianas consideradas pode ser CARACTERIZADA por rRNA 16S ou análise completa de sequência do genoma. Por exemplo, em certas modalidades, as cepas bacterianas consideradas podem compreender um rRNA 16S ou sequência genômica tendo uma certa % de identidade com uma sequência de referência.
[089] Em certas modalidades, uma bactéria modificada considerada é capaz de colonizar de forma estável o intestino humano. Uma bactéria divulgada pode, e.g., após a administração a um sujeito humano, resultar em uma abundância maior que 1012, 1011, 1010, 109, 108 ou 107 cfu por grama de conteúdo fecal. Por exemplo, a administração de cerca de 103, cerca de 104, cerca de 105, cerca de 106, cerca de 107, cerca de 108, cerca de 109, cerca de 1010, cerca de 1011 ou cerca de 1012 células de uma bactéria divulgada a um sujeito humano pode resultar em uma abundância maior que 1012, 1011, 1010, 109, 108 ou 107 cfu por grama de conteúdo fecal com 12 horas, 24 horas, 36 horas, 48 horas, 60 horas ou 72 horas de administração.
[090] Uma bactéria divulgada pode, e.g., ser modificada para colonizar o intestino humano com maior abundância, estabilidade, previsibilidade ou facilidade de colonização inicial em relação a uma bactéria similar ou de outro modo idêntica que não foi modificada. Por exemplo, uma bactéria considerada pode ser modificada para aumentar sua capacidade de utilizar um nutriente privilegiado como fonte de carbono. Um “nutriente privilegiado” é definido como uma molécula ou conjunto de moléculas que pode ser consumida para auxiliar na proliferação de uma cepa bacteriana particular, enquanto fornece assistência à proliferação a não mais que 1 % das outras bactérias no intestino. Consequentemente, em certas modalidades, uma bactéria modificada tem a capacidade de consumir o nutriente privilegiado para sustentar sua colonização e expandir no intestino de um sujeito para uma abundância previsivelmente alta, mesmo na ausência de oxalato ou outras fontes de carbono ou energia, enquanto a maioria das outras bactérias no intestino do sujeito não. Nutrientes privilegiados exemplificativos incluem, e.g., um polissacarídeo marinho, e.g., um porfirano.
[091] Por exemplo, uma bactéria pode compreender um ou mais transgenes que aumentam sua capacidade de utilizar um nutriente privilegiado, e.g., um polissacarídeo marinho, e.g., uma porfirina, como fonte de carbono. Em certas modalidades, uma bactéria pode compreender todo ou uma porção de um loco de utilização de polissacarídeos (PUL), um elemento genético móvel que confere a capacidade de consumir um carboidrato, e.g., um nutriente privilegiado, em uma bactéria. Um PUL de consumo de porfirano exemplificativo é o PUL da cepa NB001 de Bacteroides que consome porfirano representada na SEQ ID NO: 72. Consequentemente, em certas modalidades, uma bactéria modificada compreende a SEQ ID NO: 72, ou um fragmento funcional ou sua variante. Em certas modalidades, uma bactéria modificada compreende uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 72, ou um fragmento funcional ou sua variante.
[092] Modificações bacterianas exemplificativas adicionais para aumentar a abundância no intestino de um sujeito, nutriente privilegiados, transgenes que aumentam a capacidade de uma bactéria utilizar um nutriente privilegiado, PULs e outros métodos e composições para modular o crescimento de uma bactéria modificada são descritos na Publicação de Patente Internacional (PCT) No WO2018112194.
[093] Em certas modalidades, uma bactéria similar ou de outro modo idêntica que não foi modificada não tem atividade degradante de oxalato detectável e/ou não tem genes conhecidos por estarem envolvidos e.g., diretamente envolvido, no catabolismo de oxalato. Por exemplo, uma bactéria similar ou de outro modo idêntica que não foi modificada pode não ter genes que codificam: uma oxalato:formiato antiportadora (OxlT); uma oxalato descarboxilase (OXDC)- EC 4.1.1.2; uma oxalato oxidase (OXO)- EC 1.2.3.4; uma oxalato óxido-redutase (OOR)- EC 1.2.7.10; uma oxalato-CoA ligase/Oxalil-CoA sintetase (OXS)- EC 6.2.1.8; uma formil-CoA:oxalato CoA-transferase (FCOCT)- EC 2.8.3.16; uma acetil-CoA:oxalato CoA-transferase (ACOCT)- EC 2.8.3.19; uma succinil-CoA:oxalato CoA-transferase (SCOCT)- EC
2.8.3.2; uma oxalil-CoA descarboxilase (OXC)- EC 4.1.1.8; ou uma oxalil-CoA redutase/glioxilato:NADP+ óxido-redutase (OXR)- EC 1.2.1.17. Em certas modalidades, nenhum membro que ocorre naturalmente do mesmo gênero que a bactéria tem atividade degradante de oxalato detectável.
[094] Em certas modalidades, um transgene ou ácido nucleico divulgado compreendendo uma sequência exógena de nucleotídeos está operacionalmente ligado a pelo menos um promotor constitutivo, e.g., um promotor derivado de fago. O termo “operacionalmente ligado” se refere a uma ligação de elementos polinucleotídicos em uma relação funcional. Uma sequência de ácidos nucleicos está “operacionalmente ligada” quando é colocada em uma relação funcional com outra sequência de ácidos nucleicos. Por exemplo, um promotor ou realçador está operacionalmente ligado a um gene se afeta a transcrição do gene. Sequências de nucleotídeos operacionalmente ligadas são tipicamente contíguas. Entretanto, como os realçadores geralmente funcionam quando separados do promotor por várias quilobases e as sequências intrônicas podem ser de comprimentos variáveis, alguns elementos polinucleotídicos podem estar operacionalmente ligados, mas não diretamente flanqueados e podem até funcionar in trans de um alelo ou cromossomo diferente. Promotores derivados de fagos exemplificativos incluem aqueles compreendendo a sequência de nucleotídeos da SEQ ID NO: 68, SEQ ID NO: 69, SEQ ID NO: 70 ou SEQ ID NO: 71, ou uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 68, SEQ ID NO: 69, SEQ ID NO: 70 ou SEQ ID NO: 71. Promotores derivados de fagos exemplificativos adicionais são descritos na Publicação de Patente Internacional (PCT) No WO2017184565.
[095] Em certas modalidades, um transgene ou ácido nucleico divulgado compreendendo uma sequência exógena de nucleotídeos está operacionalmente ligado a pelo menos um sítio de ligação ao ribossomo (RBS). RBSs exemplificativos incluem aqueles compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 164 a 230, uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 164 a 230, ou um fragmento funcional ou variante de qualquer uma das sequências de nucleotídeos anteriores. Por exemplo, uma bactéria considerada pode compreender: (i) um transgene que codifica uma OxIT de O. formigenes operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 212 a 219, uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 212 a 219, ou um fragmento funcional ou variante de qualquer uma das sequências de nucleotídeos anteriores; (ii) um transgene que codifica uma OXS de S. cerevisiae operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 172 a 179, uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 172 a 179, ou um fragmento funcional ou variante de qualquer uma das sequências de nucleotídeos anteriores; (iii) um transgene que codifica uma OXS de A. thaliana operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 164 a 171, uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 164 a 171, ou um fragmento funcional ou variante de qualquer uma das sequências de nucleotídeos anteriores; (iv) um transgene que codifica uma OXC de S. cerevisiae operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 188 a 195 ou 220 a 230, uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 188 a 195 ou 220 a 230, ou um fragmento funcional ou variante de qualquer uma das sequências de nucleotídeos anteriores; (v) um transgene que codifica uma OXC de O. formigenes operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 180 a 187, uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 180 a 187, ou um fragmento funcional ou variante de qualquer uma das sequências de nucleotídeos anteriores; (vi) um transgene que codifica uma FCOCT de E. coli operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 204 a 211, uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 204 a 211, ou um fragmento funcional ou variante de qualquer uma das sequências de nucleotídeos anteriores; e/ou (vii) transgene que codifica uma ACOCT de E. coli operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 196 a 203, uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com qualquer uma das SEQ ID NOs: 196 a 203, ou um fragmento funcional ou variante de qualquer uma das sequências de nucleotídeos anteriores.
[096] É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 96 a 162, ou uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 96 a 162.
[097] Por exemplo, é considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 137, SEQ ID NO: 113, SEQ ID NO: 145, SEQ ID NO: 128 e SEQ ID NO: 104, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 137, SEQ ID NO: 113, SEQ ID NO: 145, SEQ ID NO: 128 e SEQ ID NO: 104. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 148, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 148, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 144, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 144, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 147, SEQ ID NO: 104, SEQ ID NO: 153, SEQ ID NO: 97, SEQ ID NO: 131, SEQ ID NO: 136 e SEQ ID NO: 113, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 147, SEQ ID NO: 104, SEQ ID NO: 153, SEQ ID NO: 97, SEQ ID NO: 131, SEQ ID NO: 136 e SEQ ID NO: 113. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105, SEQ ID NO: 113 e SEQ ID NO: 96, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105, SEQ ID NO: 113 e SEQ ID NO: 96. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105 e SEQ ID NO: 96, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105 e SEQ ID NO: 96. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 129, SEQ ID NO: 155, SEQ ID NO: 112, SEQ ID NO: 99, SEQ ID NO: 106 e SEQ ID NO: 145, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 139, SEQ ID NO: 129, SEQ ID NO: 155, SEQ ID NO: 112, SEQ ID NO: 99, SEQ ID NO: 106 e SEQ ID NO: 145. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 154, SEQ ID NO: 131, SEQ ID NO: 98, SEQ ID NO: 115, SEQ ID NO: 106 e SEQ ID NO: 149, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 139, SEQ ID NO: 154, SEQ ID NO: 131, SEQ ID NO: 98, SEQ ID NO: 115, SEQ ID NO: 106 e SEQ ID NO: 149. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 137, SEQ ID NO: 113, SEQ ID NO: 148, SEQ ID NO: 129 e SEQ ID NO: 105, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 137, SEQ ID NO: 113, SEQ ID NO: 148, SEQ ID NO: 129 e SEQ ID NO: 105 É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129 e SEQ ID NO: 105, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129 e SEQ ID NO: 105. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 144, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 155, SEQ ID NO: 137, SEQ ID NO: 128, SEQ ID NO: 144, SEQ ID NO: 115, SEQ ID NO: 98 e SEQ ID NO: 105. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105, SEQ ID NO: 113 e SEQ ID NO: 96, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105, SEQ ID NO: 113 e SEQ ID NO: 96. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105 e SEQ ID NO: 96, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 145, SEQ ID NO: 137, SEQ ID NO: 153, SEQ ID NO: 129, SEQ ID NO: 105 e SEQ ID NO: 96. É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo as sequências de nucleotídeos da SEQ ID NO: 139, SEQ ID NO: 129, SEQ ID NO: 155, SEQ ID NO: 112, SEQ ID NO: 99, SEQ ID NO: 106 e SEQ ID NO: 145, ou sequências de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99 % de identidade com a SEQ ID NO: 139, SEQ ID NO: 129, SEQ ID NO: 155, SEQ ID NO: 112, SEQ ID NO: 99, SEQ ID NO: 106 e SEQ ID NO: 145.
[098] É considerado que uma bactéria pode compreender um ou mais ácidos nucleicos compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 82 a 95, ou uma sequência de nucleotídeos tendo pelo menos 80 %, 85 %, 86 %, 87 %, 88 %, 89 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % ou 99
% de identidade com a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 82 a 95. II. Composições/Unidades Farmacêuticas
[099] Uma bactéria aqui divulgada pode ser combinada com excipientes farmaceuticamente aceitáveis para formar uma composição farmacêutica, que pode ser administrada a um paciente por quaisquer meios conhecidos na técnica. Como aqui usado, o termo “excipiente farmaceuticamente aceitável” é entendido como significando um ou mais de um tampão, portador ou excipiente adequado para a administração a um sujeito, por exemplo, um sujeito humano, sem toxicidade excessiva, irritação, resposta alérgica ou outro problema ou complicação, proporcional a uma relação risco/benefício razoável. O excipiente deve ser “aceitável” no sentido de ser compatível com os outros ingredientes das formulações e não deletério ao receptor.
[0100] Excipientes farmaceuticamente aceitáveis incluem tampões, solventes, meios de dispersão, revestimentos, agentes isotônicos e de retardamento de absorção e semelhantes, que são compatíveis com a administração farmacêutica. Excipientes farmaceuticamente aceitáveis também incluem enchedores, aglutinantes, desintegrantes, deslizantes, lubrificantes e quaisquer combinações dos mesmos. Para mais exemplos de excipientes, portadores, estabilizadores e adjuvantes, ver, e.g., Handbook of Pharmaceutical Excipients, 8a Ed., Editado por P.J. Sheskey, W.G. Cook, e C.G. Cable, Pharmaceutical Press, London, UK [2017]. O uso de tais meios e agentes para substâncias farmaceuticamente ativas é conhecido na técnica.
[0101] As bactérias consideradas podem ser usadas nas composições divulgadas em qualquer forma, e.g., uma forma estável, como conhecido pelos especialistas na técnica, incluindo em um estado liofilizado (com opcionalmente um ou mais crioprotetores apropriados), congelado (e.g., em um refrigerador padrão ou super-esfriado), seco por pulverização e/ou liofilizado. Uma formulação ou composição “estável” é uma em que o material biologicamente ativo nela essencialmente mantém sua estabilidade física, estabilidade química e/ou atividade biológica durante o armazenamento. A estabilidade pode ser medida a uma condição de temperatura e umidade selecionada por um período de tempo selecionado. A análise de tendência pode ser usada para estimar uma vida de prateleira esperada antes que um material tenha realmente sido armazenado por aquele período de tempo. Para bactérias vivas, por exemplo, a estabilidade pode ser definida como o tempo que leva para perder 1 log de cfu/g de formulação seca sob condições predefinidas de temperatura, umidade e período de tempo.
[0102] Uma bactéria aqui divulgada pode ser combinada com um ou mais crioprotetores. Crioprotetores exemplificativos incluem frutoligossacarídeos (e.g., raftilose®), trealose, maltodextrina, alginato de sódio, prolina, ácido glutâmico, glicina (e.g., glicina betaína), mono, di ou polissacarídeos (tais como glicose, sacarose, maltose, lactose), polióis (tais como manitol, sorbitol ou glicerol), dextrano, DMSO, metilcelulose, propileno glicol, polivinilpirrolidona, tensoativos não iônicos, tais como Tween 80 e/ou quaisquer combinações dos mesmos.
[0103] Uma composição farmacêutica deve ser formulada para ser compatível com a via de administração intencionada. Composições bacterianas consideradas aqui divulgadas podem ser preparadas por qualquer método adequado e podem ser formuladas em uma variedade de formas e administradas por vários meios diferentes. As composições consideradas podem ser administradas por via oral, retal ou enteral, em formulações contendo portadores, adjuvantes e veículos convencionalmente aceitáveis conforme desejado. Como aqui usado, “administração retal” é entendida como incluindo a administração por enema, supositório ou colonoscopia. Uma composição farmacêutica divulgada pode, e.g., ser adequada para administração em bolus ou liberação em bolus. Em uma modalidade exemplificativa, uma composição bacteriana divulgada é administrada oralmente.
[0104] As formas de dosagem sólidas para administração oral incluem cápsulas, tabletes, cápsulas, pílulas, pastilhas, pastilhas expectorantes, pós e grânulos. Uma cápsula tipicamente compreende um material de núcleo compreendendo uma composição bacteriana e uma parede de revestimento que encapsula o material de núcleo. Em algumas modalidades, o material de núcleo compreende pelo menos um de um sólido, um líquido e uma emulsão. Em algumas modalidades, o material da parede de revestimento compreende pelo menos um de uma gelatina mole, uma gelatina dura e um polímero. Polímeros adequados incluem, mas não são limitados a: polímeros celulósicos, tais como hidroxipropil celulose, hidroxietil celulose, hidroxipropil metil celulose (HPMC), metil celulose, etil celulose, acetato de celulose, acetato ftalato de celulose, acetato trimelitato de celulose, hidroxipropilmetil ftalato de celulose, hidroxipropilmetil succinato de celulose e carboximetilcelulose sódica; polímeros e copolímeros de ácido acrílico, tais como aqueles formados a partir de ácido acrílico, ácido metracrílico, metil acrilato, metilacrilato de amônio, etil acrilato, metil metacrilato e/ou etil metacrilato (e.g., aqueles copolímeros vendidos sob o nome comercial “Eudragit®”); polímeros e copolímeros de vinila, tais como polivinilpirrolidona, acetato de polivinila, acetato de polivinilftalato, copolímero de ácido crotônico de acetato de vinila e copolímeros de etileno-acetato de vinila; e goma laca (laca purificada). Em algumas modalidades, pelo menos um polímero funciona como um agente de mascaramento de sabor.
[0105] Tabletes, pílulas e semelhantes podem ser comprimidos, multi- comprimidos, multi-camadas e/ou revestidos. Um revestimento considerado pode ser único ou múltiplo. Em uma modalidade, um material de revestimento considerado compreende pelo menos um de um sacarídeo, um polissacarídeo e glicoproteínas extraídas de pelo menos um de uma planta, um fungo e um micróbio. Exemplos não limitativos incluem amido de milho, amido de trigo, amido de batata, amido de tapioca, celulose, hemicelulose, dextranos, maltodextrina, ciclodextrinas, inulinas, pectina,
mananos, goma arábica, goma de alfarroba, goma de mesquite, goma guar, goma karaya, goma ghatti, goma tragacanto, funori, carrageninas, porfiranos, ágar, alginatos, quitosanas ou goma gelana. Em algumas modalidades, um material de revestimento considerado compreende uma proteína. Em algumas modalidades, um material de revestimento considerado compreende pelo menos um de uma gordura e um óleo. Em algumas modalidades, o pelo menos um de uma gordura e um óleo é derretido a alta temperatura. Em algumas modalidades, o pelo menos um de uma gordura e um óleo é hidrogenado ou parcialmente hidrogenado. Em algumas modalidades, o pelo menos um de uma gordura e um óleo é derivado de uma planta. Em algumas modalidades, o pelo menos um de uma gordura e um óleo compreende pelo menos um de glicerídeos, ácidos graxos livres e ésteres de graxo ácido. Em algumas modalidades, um material de revestimento considerado compreende pelo menos uma cera comestível. Uma cera comestível considerada pode ser derivada de animais, insetos ou plantas. Exemplos não limitativos incluem cera de abelha, lanolina, cera de bayberry, cera de carnaúba e cera de farelo de arroz. Os tabletes e pílulas podem ser adicionalmente preparados com revestimentos entéricos ou entéricos reversos.
[0106] Alternativamente, os pós ou grânulos que incorporam uma composição bacteriana aqui divulgada podem ser incorporados em um produto alimentar. Em algumas modalidades, um produto alimentar considerado é uma bebida para administração oral. Exemplos não limitativos de uma bebida adequada incluem água, suco de fruta, uma bebida de fruta, uma bebida artificialmente flavorizada, uma bebida artificialmente adoçada, uma bebida carbonatada, uma bebida esportiva, um produto diário líquido, um shake, uma bebida alcoólica, uma bebida cafeinada, fórmula infantil e assim por diante. Outros meios adequados para a administração oral incluem soluções aquosas e não aquosas, emulsões, suspensões e soluções e/ou suspensões reconstituídas a partir de grânulos não efervescentes, contendo pelo menos um de solventes, conservantes, agentes emulsificadores, agentes de suspensão, diluentes, adoçantes, agentes corantes e agente flavorizantes adequados.
[0107] As composições farmacêuticas contendo uma bactéria aqui divulgada podem ser apresentadas em uma forma de dosagem unitária, i.e., uma unidade farmacêutica. Uma composição, e.g., uma unidade farmacêutica aqui fornecida, pode incluir qualquer quantidade apropriada de bactéria, medida pela massa total ou pelas unidades formadoras de colônia das bactérias.
[0108] Por exemplo, uma composição ou unidade farmacêutica divulgada pode incluir de cerca de 103 cfus a cerca de 1012 cfus, cerca de 106 cfus a cerca de 1012 cfus, cerca de 107 cfus a cerca de 1012 cfus, cerca de 108 cfus a cerca de 1012 cfus, cerca de 109 cfus a cerca de 1012 cfus, cerca de 1010 cfus a cerca de 1012 cfus, cerca de 1011 cfus a cerca de 1012 cfus, cerca de 103 cfus a cerca de 1011 cfus, cerca de 106 cfus a cerca de 1011 cfus, cerca de 107 cfus a cerca de 1011 cfus, cerca de 108 cfus a cerca de 1011 cfus, cerca de 109 cfus a cerca de 1011 cfus, cerca de 1010 cfus a cerca de 1011 cfus, cerca de 103 cfus a cerca de 1010 cfus, cerca de 106 cfus a cerca de 1010 cfus, cerca de 107 cfus a cerca de 1010 cfus, cerca de 108 cfus a cerca de 1010 cfus, cerca de 109 cfus a cerca de 1010 cfus, cerca de 103 cfus a cerca de 109 cfus, cerca de 106 cfus a cerca de 109 cfus, cerca de 107 cfus a cerca de 109 cfus, cerca de 108 cfus a cerca de 109 cfus, cerca de 103 cfus a cerca de 108 cfus, cerca de 106 cfus a cerca de 108 cfus, cerca de 107 cfus a cerca de 108 cfus, cerca de 103 cfus a cerca de 107 cfus, cerca de 106 cfus a cerca de 107 cfus, ou cerca de 103 cfus a cerca de 106 cfus de cada cepa bacteriana, ou podem incluir cerca de 103 cfus, cerca de 106 cfus, cerca de 107 cfus, cerca de 108 cfus, cerca de 109 cfus, cerca de 1010 cfus, cerca de 1011 cfus, ou cerca de 1012 cfus de bactérias. III. Usos Terapêuticos
[0109] As composições e métodos aqui divulgados podem ser usados para tratar várias doenças ou transtornos associados com uma quantidade elevada de oxalato em um sujeito. Como aqui usado, “quantidade elevada de oxalato em um sujeito” pode se referir a uma quantidade elevada de oxalato em um corpo fluido (e.g., sangue, plasma, soro ou urina), tecido e/ou célula em um sujeito, em relação a um sujeito sem a doença ou transtorno. A divulgação fornece um método de tratar uma doença ou transtorno associado com uma quantidade elevada de oxalato em um sujeito. Um método considerado compreende administrar ao sujeito uma quantidade eficaz de uma bactéria ou uma composição farmacêutica aqui divulgada, sozinha ou em uma combinação com outro agente terapêutico, para tratar a doença ou transtorno associado com uma quantidade elevada de oxalato no sujeito.
[0110] Um exemplo de uma doença ou transtorno associado com uma quantidade elevada de oxalato é hiperoxalúria, e.g., hiperoxalúria primária, entérica, dietética ou idiopática. A hiperoxalúria, ou níveis aumentados de oxalato urinário, pode ser caracterizada em humanos pela excreção urinária de oxalato maior que 40 mg (aproximadamente 440 μmol) ou 30 mg por dia. Níveis de corte clínicos exemplificativos são 43 mg/dia (aproximadamente 475 μmol) para homens e 32 mg/dia (aproximadamente 350 μmol) para mulheres. A hiperoxalúria também pode ser definida como excreção urinária de oxalato maior que 30 mg por dia por grama de creatinina urinária. Pessoas com hiperoxalúria leve podem excretar pelo menos 30 a 60 (342 a 684 μmol) ou 40 a 60 (456 a 684 μmol) mg de oxalato por dia. Pessoas com hiperoxalúria entérica podem excretar pelo menos 80 mg de oxalato urinário por dia (912 μmol), e pessoas com a hiperoxalúria primária podem excretar pelo menos 200 mg por dia (2280 μmol).
[0111] O oxalato urinário elevado tem sido associado a vários problemas de saúde nos rins, e.g., cálculos renais (e.g., cálculos renais de oxalato de cálcio recorrentes), nefrocalcinose, nefrolitíase, danos renais ou doença renal em estágio terminal. O oxalato de cálcio também pode ser depositado no trato urinário, cólon, intestino delgado, olhos, vasos sanguíneos, articulações, ossos, músculos, coração e outros órgãos importantes, causando danos aos mesmos. Consequentemente, doenças ou transtornos exemplificativos adicionais associados com uma quantidade elevada de oxalato incluindo transtornos renais (e.g., cálculos renais, nefrocalcinose, doença renal policística, nefrolitíase ou insuficiência renal (incluindo insuficiência renal progressiva, crônico ou estágio terminal)), transtornos do trato urinário (e.g., doença idiopática de cálculo urinário ou urolitíase), transtornos gastrointestinais (e.g., doença inflamatória intestinal, doença de Crohn ou colite ulcerativa), transtornos pancreáticos (e.g., insuficiência pancreática exócrina), transtornos articulares, transtornos oculares, transtornos hepáticos, envenenamento por etileno glicol, fibrose cística, esteatorreia, doença ilíaca, vulvodínia, transtornos de condutância cardíaca, osteoporose e complicações de bypass gástrico ou outros tipos de cirurgia gastrointestinal.
[0112] Como aqui usado, “tratar”, “tratando” e “tratamento” significam o tratamento de uma doença em um sujeito, e.g., em um humano. Isso inclui: (a) inibir a doença, i.e., interromper seu desenvolvimento; e (b) aliviar a doença, i.e., causar a regressão do estado da doença. Como aqui usado, os termos “sujeito” e “paciente” se referem a um organismo a ser tratado pelos métodos e composições aqui descritos. Tais organismos preferivelmente incluem, mas não são limitados a mamíferos, e.g., humanos, um animal de companhia (e.g., cão, gato ou coelho) ou um animal de criação (por exemplo, vaca, ovelha, porco, cabra, cavalo, jumento e mula, búfalo, boi ou camelo)).
[0113] Será avaliado que a dosagem exata de uma composição farmacêutica ou bactéria é escolhida por um médico individual em vista do paciente a ser tratado, em geral, a dosagem e administração são ajustados para fornecer uma quantidade eficaz do agente bacteriano ao paciente sendo tratado. Como aqui usado, a “quantidade eficaz” se refere à quantidade necessária para evocar uma resposta biológica benéfica ou desejada. Uma quantidade eficaz pode ser administrada em uma ou mais administrações, aplicações ou dosagens e não é intencionada a ser limitada a uma formulação ou via de administração particular. Como será avaliado por um especialista nesta técnica, a quantidade eficaz de uma unidade farmacêutica, composição farmacêutica ou cepa bacteriana pode variar dependendo de fatores como o ponto final biológico desejado, o fármaco a ser administrado, o tecido alvo, a via de administração, etc. Fatores adicionais que podem ser levados em conta incluem a gravidade do estado da doença; idade, peso e gênero do paciente sendo tratado; dieta, tempo e frequência de administração; combinações de fármacos; sensibilidades de reação; e tolerância/resposta à terapia.
[0114] Os métodos considerados podem compreender ainda administrar um nutriente privilegiado ao sujeito para auxiliar a colonização da bactéria. Nutrientes privilegiados exemplificativos incluem polissacarídeo marinhos, e.g., um porfirano. Por exemplo, um nutriente privilegiado divulgado pode ser administrado ao sujeito antes de, ao mesmo tempo que ou depois de uma bactéria divulgada.
[0115] Os métodos e composições aqui descritos podem reduzir os níveis de oxalato em um sujeito, e.g., em um corpo fluido (e.g., sangue, plasma, soro ou urina), tecido e/ou célula em um sujeito, em pelo menos cerca de 10 %, cerca de 20 %, cerca de 30 %, cerca de 40 %, cerca de 50 %, cerca de 60 %, cerca de 70 %, cerca de 80 %, cerca de 90 %, cerca de 95 % ou mais, em relação aos níveis de oxalato em um sujeito não tratado ou controle.
[0116] Os métodos considerados podem compreender a administração de uma bactéria ou composição farmacêutica divulgada a um sujeito a cada 12 horas, 24 horas, dia, 2 dias, 3 dias, 4 dias, 5 dias, 6 dias, 7 dias, semana, 2 semanas, 3 semanas, 4 semanas, mês, 2 meses, 3 meses, 4 meses, 5 meses ou 6 meses. Em certas modalidades, o tempo entre as administrações consecutivas de uma bactéria ou composição farmacêutica divulgada a um sujeito é maior do que 12 horas, 24 horas, 36 horas, 48 horas, 2 dias, 3 dias, 4 dias, 5 dias, 6 dias, 7 dias, 1 semana, 2 semanas, 3 semanas ou 4 semanas.
[0117] Em certas modalidades, uma bactéria divulgada e um nutriente privilegiado divulgado, e.g., um polissacarídeo marinho, e.g., um porfirano, são administrados a um sujeito com a mesma frequência. Por exemplo, a bactéria e o nutriente privilegiado podem ser administrados ao sujeito a cada 12 horas, 24 horas, dia, 2 dias, 3 dias, 4 dias, 5 dias, 6 dias, 7 dias, semana, 2 semanas, 3 semanas, 4 semanas, mês, 2 meses, 3 meses, 4 meses, 5 meses ou 6 meses. Em certas modalidades, uma bactéria divulgada e um nutriente privilegiado divulgado, e.g., um polissacarídeo marinho, e.g., um porfirano, são administrados a um sujeito com uma frequência diferente. Por exemplo, a bactéria pode ser administrada ao sujeito a cada 12 horas, 24 horas, dia, 2 dias, 3 dias, 4 dias, 5 dias, 6 dias, 7 dias, semana, 2 semanas, 3 semanas, 4 semanas, mês, 2 meses, 3 meses, 4 meses, 5 meses ou 6 meses, e o nutriente privilegiado pode ser administrado ao sujeito a cada 12 horas, 24 horas, dia, 2 dias, 3 dias, 4 dias, 5 dias, 6 dias, 7 dias, semana, 2 semanas, 3 semanas, 4 semanas, mês, 2 meses, 3 meses, 4 meses, 5 meses ou 6 meses. Por exemplo, em certas modalidades, a bactéria pode ser administrada ao sujeito a cada semana, 2 semanas, 3 semanas, 4 semanas, mês, 2 meses, 3 meses, 4 meses, 5 meses ou 6 meses, e o nutriente privilegiado pode ser administrado ao sujeito a cada 12 horas, 24 horas, dia, 2 dias, 3 dias, 4 dias, 5 dias, 6 dias ou 7 dias.
[0118] Os métodos e composições aqui descritos podem ser usados sozinhos ou em combinação com outros agentes terapêuticos e/ou modalidades. O termo administrado “em combinação”, como aqui usado, é entendido como significando que dois (ou mais) tratamentos diferentes são administrados ao sujeito durante o curso da aflição do sujeito com o transtorno, tal que os efeitos dos tratamentos no paciente se sobreponham em um determinado momento. Em certas modalidades, a liberação de um tratamento ainda está ocorrendo quando a liberação do segundo começa, de modo que existe sobreposição em termos de administração. Isso é algumas vezes referido aqui como “simultânea” ou “liberação concorrente”. Em outras modalidades,
a liberação de um tratamento termina antes da liberação do outro tratamento começar. Em certas modalidades de ambos os casos, o tratamento é mais eficaz por causa da administração combinada. Por exemplo, o segundo tratamento é mais eficaz, e.g., um efeito equivalente é observado com menos do segundo tratamento, ou o segundo tratamento reduz os sintomas a uma maior extensão do que seria observado se o segundo tratamento fosse administrado na ausência do primeiro tratamento, ou a situação análoga é observada com o primeiro tratamento. Em certas modalidades, a liberação é tal que a redução em um sintoma, ou outro parâmetro relacionado ao transtorno, é maior do que seria observado com um tratamento liberado na ausência do outro. O efeito dos dois tratamentos pode ser parcialmente aditivo, totalmente aditivo ou maior que aditivo. A liberação pode ser tal que um efeito do primeiro tratamento liberado ainda seja detectável quando o segundo é liberado. Em certas modalidades, um efeito colateral de um primeiro e/ou segundo tratamento é reduzido por causa da administração combinada.
[0119] Em certas modalidades, um método ou composição aqui descrito é administrado em combinação com um ou mais terapias adicionais. Em certas modalidades, uma terapia adicional considerada pode incluir: alta ingestão de fluidos; uma dieta de baixo teor de sal, baixo teor de oxalato e/ou um alto teor de cálcio; suplementação de citrato, cálcio, ortofosfato e/ou piridoxina/vitamina B6; a enzima oxalato descarboxilase; e/ou um peptídeo que estimula a secreção de oxalato do cólon.
[0120] Ao longo da descrição, onde as composições são descritas como tendo, incluindo ou compreendendo componentes específicos, ou onde os processos e métodos são descritos como tendo, incluindo ou compreendendo etapas específicas, é considerado que, adicionalmente, existem composições da presente divulgação que consistem essencialmente em, ou consistem em, os componentes relatados, e que existem processos e métodos de acordo com a presente divulgação que consistem essencialmente em, ou consistem em, as etapas de processamento relatadas.
[0121] No pedido, onde um elemento ou componente é dito como incluído em e/ou selecionado de uma lista de elementos ou componentes relatados, deve ser entendido que o elemento ou componente pode ser qualquer um dos elementos ou componentes relatados, ou o elemento ou componente pode ser selecionado de um grupo que consiste em dois ou mais dos elementos ou componentes relatados.
[0122] Além disso, deve ser entendido que os elementos e/ou características de uma composição ou um método aqui descrito podem ser combinados em uma variedade de maneiras sem se afastar do espírito e escopo da presente divulgação, quer explícito ou implícito neste documento. Por exemplo, onde é feita referência a um composto particular, o composto pode ser usado em várias modalidades de composições da presente divulgação e/ou em métodos da presente divulgação, a menos que de outro modo entendido a partir do contexto. Em outras palavras, dentro deste pedido, as modalidades foram descritas e representadas de uma maneira que permite que um pedido claro e conciso seja escrito e desenhado, mas é intencionado e será avaliado que as modalidades podem ser combinadas ou separadas de várias maneiras sem se separar dos presentes ensinamentos e divulgação. Por exemplo, será avaliado que todas as características descritas e representadas aqui podem ser aplicáveis a todos os aspectos da divulgação descrita e representada aqui.
[0123] Deve ser entendido que a expressão “pelo menos um de” inclui individualmente cada um dos objetos relatados depois da expressão e as várias combinações de dois ou mais dos objetos relatados a menos que de outro modo entendido a partir do contexto e uso. A expressão “e/ou” em relação a três ou mais objetos relatados deve ser entendida para ter o mesmo significado a menos que de outro modo entendido a partir do contexto.
[0124] O uso do termo “incluem”, “inclui”, “incluindo”, “têm”, “tem”, “tendo”,
“contêm”, “contém” ou “contendo”, incluindo os equivalentes gramaticais dos mesmos, deve ser entendido geralmente como aberto e não limitativo, por exemplo, não excluindo elementos ou etapas adicionais não relatados, a menos que de outro modo especificamente estabelecido ou entendido a partir do contexto.
[0125] Onde o uso do termo “cerca de” está antes de um valor quantitativo, a presente divulgação também inclui o valor quantitativo específico em si, a menos que especificamente estabelecido de outro modo. Como aqui usado, o termo “cerca de” se refere a uma variação de ±10 % do valor nominal a menos que de outro modo indicado ou inferido.
[0126] Deve ser entendido que a ordem de etapas ou ordem para realizar certas ações é imaterial, desde que a presente divulgação permaneça operável. Além disso, duas ou mais etapas ou ações podem ser conduzidas simultaneamente.
[0127] O uso de quaisquer e todos os exemplos, ou linguagem exemplificativa aqui, por exemplo, “tal como” ou “incluindo”, é intencionado meramente a ilustrar melhor a presente divulgação e não representa uma limitação no escopo da divulgação a menos que reivindicado. Nenhuma linguagem no relatório descritivo deve ser interpretada como indicando qualquer elemento não reivindicado como essencial à prática da presente divulgação.
EXEMPLOS
[0128] Os exemplos a seguir são meramente ilustrativos e não são intencionados a limitar o escopo ou conteúdo da divulgação de qualquer maneira. Exemplo 1 - Construção de Bacteroides Degradantes de Oxalato
[0129] A adição de capacidades de degradação de oxalato a um membro do gênero Bacteroides naïve de oxalato pode ser realizada pela adição de transgenes de outros organismos (e.g., SEQ ID NOs: 1 a 31). Por exemplo, um construto incluindo cassetes de expressão para a expressão de OxlT, OXS e OXC pode ser usado para obter a degradação de oxalato em Bacteroides. Sequências de codificação de OxIT exemplificativas são representadas nas SEQ ID NOs: 17 a 21, sequências de codificação de OXS exemplificativas são representadas nas SEQ ID NOs: 14 a 16 e sequências de codificação de OXC exemplificativas são representadas nas SEQ ID NOs: 22 a 26. Os construtos considerados podem ainda incluir cassetes de expressão para a expressão de FCOCT, ACOCT ou SCOCT. Sequências de codificação de FCOCT exemplificativas são representadas nas SEQ ID NOs: 4 a 8, sequências de codificação de ACOCT exemplificativas são representadas nas SEQ ID NOs: 1 a 2 e sequências de codificação de SCOCT exemplificativas são representadas nas SEQ ID NOs: 28 a 31. A expressão de cópias adicionais de OXS e/ou OXC também pode melhorar a degradação de oxalato.
[0130] A FIGURA 3 mostra plasmídeos diferentes (SEQ ID NOs: 63 a 67) que podem ser usados individualmente ou em combinação para gerar Bacteroides com capacidades de degradação de oxalato. pZR748 (SEQ ID NO: 63) inclui sequências de codificação para uma OXS de S. cerevisiae, uma OXC de S. cerevisiae e uma OxIT de O. formigenes. pZR753 (SEQ ID NO: 64) inclui sequências de codificação para uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae e uma OxIT de O. formigenes. pZR761 (SEQ ID NO: 65) inclui sequências de codificação para uma OXS de S. cerevisiae e uma OXC de S. cerevisiae. pZR856 (SEQ ID NO: 66) inclui uma sequência de codificação para uma OxIT de O. formigenes. pZR878 (SEQ ID NO: 67) inclui sequências de codificação para uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma OXS de A. thaliana e uma ACOCT de E. coli.
[0131] Cada cópia de um gene relacionado ao catabolismo de oxalato foi operacionalmente ligada a uma cópia de um promotor derivado de fago, P_BfP1E6 (SEQ ID NO: 68) ou uma versão enfraquecida do mesmo, P_BfP4E5 (SEQ ID NO: 69). Outros promotores, incluindo P_BfP2E5 (SEQ ID NO: 80), e outros que correspondem a uma sequência consenso derivada de fago (SEQ ID NO: 70), também podem ser usados. Para otimizar a taxa de tradução das enzimas, as enzimas foram fundidas a um peptídeo líder acoplado a um sítio de ligação ao ribossomo de alta força (RBS) (SEQ ID NO: 78) ou uma enzima repórter de luciferase NanoLuc acoplada a um RBS de alta força (SEQ ID NO: 79). Outros sítios de ligação ao ribossomo e fusões traducionais também podem ser usados. Os plasmídeos carregam um gene NBU2 integrase, que catalisa a integração genômica do plasmídeo na extremidade 3’ de um gene serina-tRNA no genoma de Bacteroides.
[0132] Uma vez construídos, os plasmídeos foram transformados em células doadoras de Escherichia coli S17-1 portadoras de plasmídeo conjugativo RK2. A maquinaria conjugativa RK2 permite a transferência conjugativa de plasmídeos com uma origem de transferência de RP4 para uma variedade de espécies, incluindo Bacteroides. Essas células foram misturadas com células receptoras de Bacteroides e plaqueadas em placas de ágar BHI não seletivas para uma incubação durante a noite a 37 °C. Posteriormente, as placas foram raspadas e a mistura de E. coli S17-1 e Bacteroides foi transferida para meios seletivos com gentamicina para suprimir a E. coli e cultivada sob condições anaeróbicas. Múltiplos marcadores seletivos (tais como tetraciclina, eritromicina ou cloranfenicol) podem ser usados para entregar múltiplos construtos em uma única cepa de Bacteroides. Exemplo 2 - Teste In vitro De Degradação De Oxalato Em Cultura Líquida
[0133] Os Bacteroides projetados, construídos como descrito no Exemplo 1, foram avaliados para a capacidade de degradação de oxalato em um ensaio in vitro. As células de Bacteroides foram diluídas de uma cultura durante a noite 1:10 em meios líquidos BHIS com 10 ou 30 mM adicionados de oxalato de sódio. As culturas foram incubadas anaerobicamente a 37 °C durante a noite. Depois de 48 horas, as culturas foram centrifugadas para separar as células, e o sobrenadante foi retirado para análise. As amostras foram analisadas usando um kit oxalato oxidase de 595 nm (Sigma-Aldrich, Catálogo # MAK315-1KT) e comparadas com uma curva padrão de modo a calcular as taxas de consumo de oxalato. Os dados resultantes dos plasmídeos de teste pZR748 (SEQ ID NO: 63), pZR753 (SEQ ID NO: 64), pZR753 + pZR761 (SEQ ID NOs: 64, 65) e pZR856 + pZR878 (SEQ ID NOs: 66, 67) em um ambiente de Bacteroides vulgatus ATCC 8482 são mostrados na FIGURA 4. Como representado, o consumo de oxalato de cada cepa projetada foi aumentado em relação ao tipo selvagem, que não mostrou capacidade para consumo de oxalato. Exemplo 3 - Engenharia De Consumo de Nutriente Privilegiado Em Bacteroides
[0134] Um loco de utilização de polissacarídeos (PUL) é um elemento genético móvel que confere a capacidade de consumir novos carboidratos a uma bactéria. Um PUL de consumo de porfirano foi identificado no isolado da cepa NB001 de Bacteroides que consome porfirano (SEQ ID NO: 72) e uma região 60kb do PUL foi clonada em um cromossomo bacteriano artificial (BAC) para criar pWD035 (SEQ ID NO: 73). O conteúdo de pWD035 é mostrado na FIGURA 5.
[0135] O plasmídeo pWD035 foi conjugado em Bacteroides vulgatus ATCC 8482 usando E. coli S17-1 para gerar a cepa NB075. Essa cepa foi testada para sua capacidade de consumir o polissacarídeo marinho porfirano. As células foram diluídas 1:50 de uma cultura durante a noite em meios mínimos de Salyer contendo 0,2 % de extrato de porfirano de nori Porphyra yezoensis. Durante 14 horas de incubação anaeróbica a 37 °C, a OD600, representando o crescimento de células, foi medida usando um leitor de placa. Os resultados, mostrados na FIGURA 6, demonstraram que apenas as células que receberam o PUL porfirano ganharam a capacidade de consumir porfirano para crescimento. Exemplo 4 - Cepas de Engenharia Que Possuem Consumo De Oxalato E Um Nutriente privilegiado
[0136] A FIGURA 7 mostra plasmídeos (SEQ ID NO: 75-77) que podem ser usados individualmente ou em combinação para gerar Bacteroides com capacidades de degradação de oxalato de alta eficiência. pZR0879 (SEQ ID NO: 75) inclui sequências de codificação para uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OxIT de O. formigenes. pZR0901 (SEQ ID NO: 76) inclui sequências de codificação para uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma OXS de A. thaliana, uma ACOCT de E. coli e uma OxIT de O. formigenes. pZR1871 (SEQ ID NO: 77) inclui sequências de codificação para uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma OXS de A. thaliana, uma ACOCT de E. coli e uma OxIT de O. formigenes.
[0137] Cepas que são capazes de consumir simultaneamente porfirano e oxalato foram geradas pela conjugação e plaqueamento seletivo de plasmídeos pZR0879, pZR0901 e pZR1871 na cepa NB075 (descrito no Exemplo 3). Um vetor vazio (pZR1622, SEQ ID 74) também foi incluído de modo a construir uma cepa controle que não consome oxalato. Para duas das conjugações (pZR879 e pZR1871), de modo a encontrar cepas com taxas aumentadas de consumo de oxalato, ~3.000 colônias foram isoladas de uma placa seletiva individual. Esses isolados clonais foram então submetidos a uma versão modificada do ensaio de consumo de oxalato descrito no Exemplo 1, que foi conduzido em alto rendimento em um robô de manipulação de líquido acústico Labcyte Echo 525. Aproximadamente 0,001 % dos isolados clonais provaram ser outliers que demonstraram taxas de consumo de oxalato significativamente mais rápidas que a maioria dos isolados. O sequenciamento do genoma inteiro em um Illumina iSeq 100 foi usado para confirmar que essas cepas continham duas cópias do plasmídeo de interesse em seu genoma, presumivelmente levando à expressão aumentada dos genes da via de oxalato. Os detalhes das cepas geradas, incluindo o número de cópias de plasmídeos, são representados na Tabela
1.
TABELA 1 - Cepas de Bacteroides projetadas para consumir porfirano e oxalato Nome da Cepa Nome do SEQ Cópias Consumidora Consumidora Cepa Parental Plasmídeo ID Integradas de Porfirano de Oxalato B. vulgatus - - - - Não Não 8482 B. NB075 vulgatus pWD035 73 1 Sim Não 8482 NB124 NB075 pZR1622 74 1 Sim Não sWW152 NB075 pZR0901 76 1 Sim Sim NB120 NB075 pZR1871 77 1 Sim Sim sWW554 NB075 pZR0879 75 2 Sim Sim sWW626 NB075 pZR1871 77 2 Sim Sim
[0138] As quatro cepas que consomem oxalato (sWW152, NB120, sWW554 e sWW626) foram testadas em um ensaio in vitro de consumo de oxalato para comparar suas respectivas taxas de consumo de oxalato. Cada cepa foi cultivada para saturação anaerobicamente em meios BHIS a 37 °C. As culturas foram então peletizadas e recolocadas em suspensão em um décimo do volume inicial com BHIS frescos que tinham sido suplementados com 4mM de oxalato. O plaqueamento de diluição das culturas concentradas de 10 vezes indicou que elas continham ~1x1010 cfu/ml, que é a abundância aproximada que essas cepas atingem no ceco quando deixadas para colonizar o intestino. As culturas concentradas foram transferidas de volta para uma câmara anaeróbica e deixadas incubar a 37 °C. Em intervalos regulares, as alíquotas de cultura foram removidas da câmara anaeróbica, transferidas para gelo, peletizadas e o sobrenadante foi congelado. As alíquotas de sobrenadante foram então medidas para determinar a concentração de oxalato remanescente. Os resultados são representados na FIGURA 8. Como esperado, cada cepa reduziu a concentração de oxalato no sobrenadante, com sWW626, consumindo 75 % do oxalato dentro de 15 minutos. Exemplo 5 - Teste Ex Vivo De Taxas De Consumo De Oxalato Em Conteúdos
Cecais de Rato
[0139] As cepas que consomem oxalato/porfirano candidatas descritas no Exemplo 4 foram em seguida submetidas a um ensaio ex-vivo de consumo de oxalato projetado para simular as condições in vivo o mais próximo possível. Primeiro, os cecos de múltiplos ratos Sprague-Dawley alimentados com uma dieta de baixo teor de oxalato foram colhidos e os conteúdos foram extrusados e diluídos 3 vezes em água destilada para ajudar na pipetagem. O oxalato foi adicionado a uma concentração final de 4mM. Cepas que consomem oxalato foram então adicionadas a uma concentração final de 1x1010 cfu/ml para corresponder à sua abundância esperada in vivo no ceco. O consumo de oxalato com o passar do tempo foi monitorado como descrito acima. Os resultados são representados na FIGURA 9. Novamente, cada cepa consumiu oxalato nos conteúdos cecais, embora a taxa de consumo de oxalato nos conteúdos cecais fosse ~10 vezes mais lenta que tinha sido em meios BHIS. Exemplo 6 - Eficácia In Vivo Em Um Modelo De Rato De Hiperoxalúria Secundária
[0140] Para demonstrar que as cepas de Bacteroides que consomem oxalato podem ser usadas para tratar hiperoxalúria, cepas candidatas foram submetidas a teste in vivo em um modelo de rato de hiperoxalúria secundária. A hiperoxalúria foi induzida através de uma dieta de baixo teor de cálcio que causa absorção excessiva de oxalato dietético. A FIGURA 10 e a FIGURA 11 representam os resultados de experimentos in vivo projetados para testar a eficácia de cepas terapêuticas candidatas sWW152 e sWW554, respectivamente. Os ratos Sprague-Dawley machos pesando aproximadamente duzentos gramas foram alojados individualmente em gaiolas metabólicas (Tecniplast, 3700M022) e alimentados com uma dieta básica de baixo teor de cálcio (<0,01 %) e baixo teor de oxalato (Envigo, TD.160869). A urina de vinte e quatro horas foi coletada diariamente e analisada para o oxalato total ao longo de cada experimento. Começando no dia três e continuando pelo restante do experimento, 52 µmol de oxalato por dia (FIGURA 10) ou 30 µmol de oxalato por dia (FIGURA 11) na forma de espinafre fresco foram adicionados à dieta básica, resultando em hiperoxalúria. Começando no dia dois (FIGURA 10) ou dia sete (FIGURA 11) e continuando pelo restante do experimento, 780 mg porfirano/dia também foram adicionados à dieta básica. No dia sete, uma cepa controle incapaz de degradar oxalato (NB075 na FIGURA 10 ou NB124 na FIGURA 11) ou a cepa terapêutica de degradação de oxalato (sWW152 na FIGURA 10 ou sWW554 na FIGURA 11) foi administrada adicionando-se 109 cfu à água potável. A adição de sWW152 resultou em uma redução de 32 % no oxalato na urina em comparação com a cepa controle (FIGURA 10, p=0,005), enquanto a adição da cepa sWW554 melhorada resultou em uma redução de 46 % no oxalato na urina em comparação com a cepa controle (FIGURA 11, p=0,0003). Ambas as cepas controle e terapêuticas colonizaram o intestino do rato em alta abundância na presença de porfirano, perfazendo aproximadamente 3*109 cfu/mL de fezes dentro de poucos dias (FIGURA 12). Esses resultados demonstram a capacidade de Bacteroides de degradação de oxalato projetados para reduzir o oxalato urinário em um modelo de roedor de hiperoxalúria secundária. Além disso, dado que a cepa sWW554 teve um impacto terapêutico maior que a sWW152, a taxa crescente de consumo de oxalato da cepa pareceu se traduzir em eficácia in vivo melhorada. Exemplo 7 - Eficácia In Vivo Em Um Modelo De Rato De Hiperoxalúria Secundária
[0141] Para demonstrar que as cepas de Bacteroides que consomem oxalato podem ser usadas para tratar hiperoxalúria, as cepas candidatas foram submetidas a teste in vivo em um modelo de rato de hiperoxalúria secundária.
[0142] sWW626 (como descrita no Exemplo 4) foi modificada para conter um cassete de resistência a eritromicina para criar a cepa sWW627. A FIGURA 13A e a
FIGURA 13B representam os resultados de experimentos in vivo projetados para testar a eficácia de cepas terapêuticas candidatas sWW627.
[0143] Ratos Sprague-Dawley machos pesando aproximadamente seiscentos gramas foram alojados individualmente em gaiolas metabólicas (Tecniplast, 3701M081) e alimentados com uma dieta básica de alto teor de gordura (40 %) contendo 86 µmol de oxalato diário por rato. A urina de vinte e quatro horas foi coletada diariamente e analisada para o oxalato total ao longo de cada experimento. O oxalato basal na urina foi medido nos dias 1 e 2. Começando no dia três e continuando pelo restante do experimento, espinafre foi adicionado à dieta básica, resultando em hiperoxalúria. No dia 7, os ratos foram inoculados com nenhuma cepa, uma cepa controle capaz de crescer em porfirano, mas sem os genes de consumo de oxalato (NB124) ou uma cepa terapêutica capaz de crescer em porfirano e consumir oxalato (sWW627). As cepas foram administradas adicionando-se 109 cfu à água potável. Começando no dia 12, porfirano foi adicionado à água potável (e aumentado ao longo do curso do experimento como mostrado na FIGURA 13A e na FIGURA 13B.)
[0144] A abundância de cepa, medida pela enumeração de unidades formadoras de colônia (CFU) por grama de fezes, é mostrada na FIGURA 13A. Como representado, um aumento na abundância de cepa correspondeu a maiores concentrações de porfirano na dieta. O oxalato na urina é mostrado na FIGURA 13B. Como representado, a administração da cepa sWW627 reduz o oxalato na urina em relação a nenhuma cepa ou a cepa controle. A redução no oxalato na urina pela sWW627 aumentou conforme as concentrações de porfirano na dieta aumentaram. Exemplo 8 - Eficácia In Vivo Em Um Modelo De Rato De Hiperoxalúria Secundária
[0145] Para demonstrar que as cepas de Bacteroides que consomem oxalato podem ser usadas para tratar hiperoxalúria, as cepas candidatas foram submetidas a teste in vivo em um modelo de rato de hiperoxalúria secundária.
[0146] A hiperoxalúria foi induzida em ratos através de cirurgia de Bypass gástrico em Y-de-Roux (RYGB). Os ratos foram alimentados com uma dieta que incluiu porfirano, cálcio e espinafre. A urina de cada animal foi coletada e o volume e a concentração de oxalato foram medidos a cada dia. A concentração de oxalato foi medida por meio do Kit EnzyChrom Oxalate Assay (BioAssay Systems, Hayward, CA). Os ratos foram administrados com uma cepa terapêutica capaz de crescer em porfirano e consumir oxalato (sWW627, descrita no Exemplo 7) ou uma cepa controle.
[0147] Os resultados são representados na FIGURA 14. Os ratos que receberam cirurgia de RYGB e foram administrados com uma cepa controle mostraram um nível mais alto de oxalato na urina que os ratos que foram submetidos a uma cirurgia simulada. Os ratos que receberam a cirurgia de RYGB e foram administrados com uma cepa terapêutica (sWW627) mostraram oxalato reduzido na urina em relação aos ratos administrados com a cepa controle e reverteram os impactos da cirurgia de RYGB. Exemplo 8 - Engenharia De Sítios De Ligação Ao Ribossomo (RBSs)
[0148] Os sítios de ligação ao ribossomo (RBSs) foram triados a partir de uma biblioteca sintética para produzir um conjunto de sequências de RBS que pode ser usado para ajustar a expressão de genes de degradação de oxalato. Para cada gene de consumo de oxalato de interesse, mais de 1000 sequências de RBS foram triadas de uma biblioteca onde a sequência degenerada NNNNNNNNWWWAAAWWTWANAAA foi localizada imediatamente a montante do sítio de partida de tradução de cada gene. Cada gene também foi traducionalmente fundido ao terminal C a uma proteína luciferase. A montante de cada RBS, a transcrição foi conduzida pelo promotor P_BfP1E6 (SEQ ID NO: 68). Os plasmídeos contendo o cassete de expressão foram genomicamente integrados em Bacteroides vulgatus e clones individuais de RBSs únicos foram cultivados para saturação em meios BHIS. A luminescência de cada cultura saturada foi medida com o Nano-Glo Luciferase Assay System (Promega Corporation, Madison, WI).
[0149] A FIGURA 15A descreve os valores de luminescência para cada clone de cada gene indicado. Os clones selecionados foram isolados e sequenciados para determinar cada sequência de RBS de interesse. As SEQ ID NOs: 96 a 103 representam uma sequência de codificação de OXS de A. thaliana com oito RBSs de interesse. Os RBSs nas SEQ ID NOs: 96 a 103 são referidos como RBS1-8, respectivamente. As SEQ ID NOs: 104 a 111 representam uma sequência de codificação de OXS de S. cerevisiae com oito RBSs de interesse. Os RBSs nas SEQ ID NOs: 104 a 111 são referidos como RBS1-8, respectivamente. As SEQ ID NOs: 112 a 119 representam uma sequência de codificação de OXC de O. formigenes com oito RBSs de interesse. Os RBSs nas SEQ ID NOs: 112 a 119 são referidos como RBS1-8, respectivamente. As SEQ ID NOs: 120 a 127 representam uma sequência de codificação de OXC de S. cerevisiae com oito RBSs de interesse. Os RBSs nas SEQ ID NOs: 120 a 127 são referidos como RBS1-8, respectivamente. As SEQ ID NOs: 128 a 135 representam uma sequência de codificação de ACOCT de E. coli com oito RBSs de interesse. Os RBSs nas SEQ ID NOs: 128 a 135 são referidos como RBS1-8, respectivamente. As SEQ ID NOs: 136 a 143 representam uma sequência de codificação de FCOCT de E. coli com oito RBSs de interesse. Os RBSs nas SEQ ID NOs: 136 a 143 são referidos como RBS1-8, respectivamente. As SEQ ID NOs: 144 a 151 representam uma sequência de codificação de OxIT de O. formigenes com oito RBSs de interesse. Os RBSs nas SEQ ID NOs: 144 a 151 são referidos como RBS1-8, respectivamente. As SEQ ID NOs: 152 a 162 representam uma sequência de codificação de OXC de S. cerevisiae de códon otimizado com onze RBSs de interesse. Os RBSs nas SEQ ID NOs: 152 a 162 são referidos como RBS1-8, RBS1b, RBS2b e RBS3b, respectivamente.
[0150] Os plasmídeos foram reconstruídos com a sequência acima e genomicamente incorporados em Bacteroides vulgatus. A luminescência das culturas foi avaliada como descrito acima. Os resultados são mostrados na FIGURA 15B.
[0151] Exemplo 9 - Teste In Vitro De Degradação De Oxalato Em Cultura Líquida
[0152] Os Bacteroides projetados foram avaliados para a capacidade de degradação de oxalato em um ensaio in vitro.
[0153] As células Bacteroides foram diluídas a partir de uma cultura durante a noite 1:10 em meios líquidos BHIS com 16 mM adicionados de oxalato. As culturas foram incubadas anaerobicamente a 37 °C. Depois de 20 horas, as culturas foram centrifugadas para separar as células e o sobrenadante foi retirado para análise. As amostras foram analisadas usando um kit oxalato oxidase de 595 nm (Sigma-Aldrich, Catálogo # MAK315-1KT) e comparadas com uma curva padrão de modo a calcular as taxas de consumo de oxalato.
[0154] As cepas testadas incluíram NB136 (não contendo nenhum gene de consumo de oxalato introduzido), sWW152 (como descrito no Exemplo 4), sWW627 (como descrito no Exemplo 7) e sWW1077. SWW1077 contém a SEQ ID NO: 82, que inclui uma via de consumo de oxalato projetada junto com os RBSs projetados identificados no Exemplo 8 (incluindo RBS2-FCOCT de E. coli (SEQ ID NO: 137), RBS2-OXC de O. formigenes (SEQ ID NO: 113), RBS2-OxIT de O. formigenes (SEQ ID NO: 145), RBS1-ACOCT de E. coli (SEQ ID NO: 128) e RBS1-OXS de S. cerevisiae (SEQ ID NO: 104)). Os resultados são mostrados na FIGURA 16. Como representado, o consumo de oxalato de cada cepa projetada foi aumentado em relação à cepa controle e o maior consumo de oxalato foi observado para sWW1077.
INCORPORAÇÃO POR REFERÊNCIA
[0155] A divulgação completa de cada uma das patentes e documentos científicos referidos aqui é incorporada por referência para todos os propósitos.
EQUIVALENTES
[0156] A divulgação pode ser incorporada em outras formas específicas sem se afastar do espírito ou características essenciais da mesma. As modalidades anteriores devem, portanto, serem consideradas, em todos os aspectos, ilustrativas em vez de limitativas da divulgação aqui descrita. O escopo da divulgação é, portanto, indicado pelas reivindicações anexas em vez da descrição anterior e todas as alterações que vêm dentro do significado e intervalo de equivalência das reivindicações são intencionados a estarem abrangidos nas mesmas.
LISTAGEM DE SEQUÊNCIAS
[0157] SEQ ID NO: 1 (Acetil-CoA:oxalato CoA-transferase (ACOCT) de cepa Acetobacter aceti 1023; EC 2.8.3.19; Acesso UniProt A9X6P9; Aa_CoAT3):
ATGACTGAAACCACGCCAGCAAAACCGAAGGGGCCCTTTGATGGGCTC CTCGTTATTGATCTGACACATGTGCTGAATGGTCCGTTCGGCACAACAATTCTAA CAGATCTGGGGGCTCGTACAATCAAGATCGAGCCACCGGGGCATGGGGATGAT ACGCGTACCTATGGCCCATATGTTGGAGATCAATCGCTTTACTTCTCTTTTGTAA ACAGGGGAAAGGAAAGCATTGTCCTTAACCTCAAGGATGAGGGCGATCGAGCG ATTTTTCTGGAAATGGTGCGTAAGGCCGATGTGCTGGCGGAAAACTTCCGCCCC GGCGTGATGGACCGTTTGGGTTTCAATTATGAGGAACTGGCAAAGATCAATCCA CGGTTGATTTATGCATCTTCATCAGGGTTCGGTCAGACAGGCCCGCTCGCACAT TATCCTGCCTACGATACTATTGTGCAGGCCATGAGCGGCATCATGATGGCCACG GGCTTTCCCGATGGTCCGCCAACGCGGGTAGGGGGCACATCGCTTTCTGATCT GTGCGGTGGGGTTTTCATGTTCTGCGGCATTGCCAGTGCGCTTTATGCGCGTG AGCGCACAGGGAAAGGGGCACACATTGATGTTTCAATGTTTGATGGTACCTTGG CATTTTTGCAGCATGCTCTGATGTGCTGGTCTGCTACAGGCAAAGCCCCTGCGC GTATTGGCAATCGCCATCCTTATATGGCGCCTTTTGATGTGTTTCAGGCGCAGG ACAAGCCTTTTGTCATCTGTTGTGGGAATGACCATCTTTTTAAGGCGCTTTGCGA TGTGATTGGTGCGCCTGAACTGGCGACAGACCCGCGTTTTGTCGAAAATCATGA TAGAATGGCCAATAATGATGCGCTGAAAGCTGCATTGGAAAAAGCACTTTCCAA ACAGCCAGCGGCGCATTGGCTGGATGTTATTCACAAAGCCGGTGTGCCTGTTG GGCCGTTGCTGGACGTGGCAGAGGCGGCAAATCTGCCACAAACAGCCGCGCG GAATATGCTGATTAAATCTGGCGGGGTGATGATGCCCGGTAACCCGGTGAAAAT CAGTGGGTATGATGACCCACATGAACGGCCCGGCGCCCCTAAGCTGGATGAGC AGGGCGCGGCGTTGCGTAAGGAATTTGCCGCACCAGAGGCAAAATGA
[0158] SEQ ID NO: 2 (Acetil-CoA:oxalato CoA-transferase (ACOCT) de Escherichia coli MG1655; EC 2.8.3.19; Acesso UniProt P76518; Ec_yfdE):
ATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCG ATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGG CGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATT TGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAA GAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATAT GCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAA ACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGC TTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGA TACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGC TCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTT ATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGG CGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTC TGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATC CCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTG TTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGA ACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCT ATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGT TAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTG AGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGG GAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCAT GTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGA GTTCTCATCATAA
[0159] SEQ ID NO: 3 (Formiato desidrogenase de Saccharomyces cerevisiae S288C; EC 1.2.1.2; Acesso UniProt Q08911; Sc_FDH1):
ATGTCGAAGGGAAAGGTTTTGCTGGTTCTTTATGAAGGTGGTAAGCATG CTGAAGAGCAGGAAAAGTTATTGGGGTGTATTGAAAATGAACTTGGTATCAGAA ATTTCATTGAAGAACAGGGATACGAGTTGGTTACTACCATTGACAAGGACCCTG AGCCAACCTCAACGGTAGACAGGGAGTTGAAAGACGCTGAAATTGTCATTACTA CGCCCTTTTTCCCCGCCTACATCTCGAGAAACAGGATTGCAGAAGCTCCTAACC TGAAGCTCTGTGTAACCGCTGGCGTCGGTTCAGACCATGTCGATTTAGAAGCTG CAAATGAACGGAAAATCACGGTCACCGAAGTTACTGGTTCTAACGTCGTTTCTG TCGCAGAGCACGTTATGGCCACAATTTTGGTTTTGATAAGAAACTATAATGGTGG TCATCAACAAGCAATTAATGGTGAGTGGGATATTGCCGGCGTGGCTAAAAATGA GTATGATCTGGAAGACAAAATAATTTCAACGGTAGGTGCCGGTAGAATTGGATA TAGGGTTCTGGAAAGATTGGTCGCATTTAATCCGAAGAAGTTACTGTACTACGA CTACCAGGAACTACCTGCGGAAGCAATCAATAGATTGAACGAGGCCAGCAAGCT TTTCAATGGCAGAGGTGATATTGTTCAGAGAGTAGAGAAATTGGAGGATATGGT TGCTCAGTCAGATGTTGTTACCATCAACTGTCCATTGCACAAGGACTCAAGGGG TTTATTCAATAAAAAGCTTATTTCCCACATGAAAGATGGTGCATACTTGGTGAATA CCGCTAGAGGTGCTATTTGTGTCGCAGAAGATGTTGCCGAGGCAGTCAAGTCT GGTAAATTGGCTGGCTATGGTGGTGATGTCTGGGATAAGCAACCAGCACCAAAA GACCATCCCTGGAGGACTATGGACAATAAGGACCACGTGGGAAACGCAATGAC TGTTCATATCAGTGGCACATCTCTGGATGCTCAAAAGAGGTACGCTCAGGGAGT AAAGAACATCCTAAATAGTTACTTTTCCAAAAAGTTTGATTACCGTCCACAGGAT ATTATTGTGCAGAATGGTTCTTATGCCACCAGAGCTTATGGACAGAAGAAATAA
[0160] SEQ ID NO: 4 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Bifidobacterium animalis subsp. lactis DSM 10140; EC 2.8.3.16; Acesso UniProt
B9X295; Ba_frc):
ATGGCTGACAAAAGCACTGCCCCACTGGCAGGCATCAAGGTAATCGAC TGGACGCAGGTTCAGTCTGGCCCTTCGTGCACCCAGATTCTCGCATGGCTGGG CGCCGAGGTCATCAAGCTCGAGAAGGTGCATGGCGGCGACCCGACCCGTAAC GAGATGAACGACGTCGACGGCTCGTACTCGCTGTACTTCCTGCAGCTCAACGC CAACAAGAAGTCGATCACGCTCGACATGAAGGACCCGGAAGGCAAGAAGATCC TCACCGACCTGCTCAAGGACGCAGACGTGTTCGTAGAGAACATCGGCCCAGGC GACGTGGAAAAGCTCGGCTTCGGTTGGGACGAGGTCCACAAGATCAACCCGAA GCTCATCATGGCCTCGCTCAAGGGATTCAACCAGGGCAGCCGCTTCGAACATG TCAAGGCCTTCGAGCCTGTCGCCCAGTGCGCAGGCGGCGCTGCTTCGACCACC GGCTGGTGGGAAGGCGACAAGAACATCCCGACGCAGTCCGGCGCTGCCCTTG GTGATTCGAACACCGGTATGCACCTGACGATTGCAATCCTCACCGCACTGCTGC AGCGCGAGCGCACCGGCGAAGGCGTGTTCGTGTACCAGTCCATGCAGAATGCA GTGCTCAACCTATGCCGCATCAAGCTGCGTGATCAGCTCATTCTCGATCACCTA CACCAGCTTTCCTACTACGACTGCTACCCGGGCTACAAGTTCGGCAAAGCGATT CCGCGTGCGGCCAATGCCGAGGGCGGACTCGTGCTCGGCTGGTGCTATCGCG CCAAGGGCTGGGAAACCGATCCGAACGCCTATGTGTACATTGTGATTCAGCAGT CGCAGAAGGGATTCGAGAACTTCTGCAACGCCATGGGCTTCCAGGATTGGCTC ACCGATCCGAAGTTCAGCACGCCGAACGCTCGCGATGAGCACAAGCAGGAAGT GTACAAGCGCGTCGAGGAATACACGATGCAGTACGACAAGTACACGCTCACCA AGGAACTCGGCGCCAAGGGCGTTCCGGTGGGCCCGGTGCTCGATTGGAACGA ACTCGAAAACGATCCGGATCTCAACGAGGATGGCACGCTGATCACGATCGATCA GGGCGACGCCCGCGGCAAGTTCAAGACGATCGGCCTGCCGTTCACGATGAGC AACTATGCGCCGGATTACCAGCGCGCGCCGAAGCTCGGCGAGAACAACGAGG AGATTCTCAAGTCCCTCGGCTACACCGACGAGCAGATCGCCGATCTGGCGACC AAGGGCGTCATCGGCTCGAACGATGGCGTCAAGGCAGACCTCACCGCCGCTCC GGCACAGGCCTGA
[0161] SEQ ID NO: 5 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.16; Co_frc1):
ATGCCTGCACAACACACCCATGCCACGCCCGCCGGCCCGCTCGACGG CATCCGCGTGCTCGACCTGACCGCGGTCGTGCTCGGCCCGCTGGCCACGCAG GTGCTGGCCGATTTTGGCGCCGACGTGATCAAGATCGAGGGGCCGGAAGGCG ACCTGATGCGCGCCAACGGCGTGTCGCAGCACGCCGGCATGAGCTCGATCTAC CTGGCGCTGAACCGCAACAAGCGCTCGGTCGTGCTCGACCTGAAAATCCCCGA GGGCGCCGAAGCGCTGCGCGCGCTGATCGCCGGCGCCGACGTGCTGGTGCA CAACATGCGCGTGGCGGCGATCGAGCGGCTTGGTTTTGGCTACGAGGCCGTC GCACGCATCAACCCGCGCATCGTCTACTGCGTCGCCACCGGCTTCGGCCAGGA CGGCCCGCACCGCGACAAGCCCGCCTTCGACGACATCATCCAGGCCGGCTGC GGACTGGTGGCGCTGGGCGCCGGCAACGGCGAGCGGCCGGAATACGTGCCC AGCCTGATCGCCGACAAGACCACCGGGCTGGCGCTGGCCAATGCCGTGCTGG CCGCGCTGCTGCACCGCGAACGCCATGGCGTGGGCCAGTCGGTCGAAGTGCC GATGCTGGAAACCATGGCCGCCTTCGTGATGACCGAGCACCTGGGCGGGCTGA CCTTCGAGCCCGCCCCCGCCGGCGCGGGCTATGCGCGGCTACTGCAGGGCGG CCGCCGCCCGGCGCCGACGCGCGACGGCTGGATCTGCGCGCTGCCCTATACC GAGCGCCACTGGCATGCCTTCTTCCGCGCGGTGGGTCGCGATGACCTTGCCGA CCGTTACGAAGTCGGCGACCGCGCACAGCGCAATGCCAATATCCGCGCGCTGT ACGGCCATCTTGCCGAACTGACCCCGGAGCGCAGCACCGCAGAATGGATGGC GCTGTTCGAAGCGCTCGACATCCCCGCCACGCCGATCTACGACCTCGATGCGC TGGTCGACCATCCGCACCTGCGCGCGGTGGGACTGTTCCAGGCCACGCAGCA CCCCACCGAAGGCCCGCTGCGCGAAGTGCGCCCGGCCGCGCGCTTCTCGGCC ACGCCGCTGTCGTTGCGCCGGCATGCGCCGGCACTGGGCGAACACACCGCCG AAGTCTTGCAGGAAGCCGGCATCGCCCTGCCCGCCGCGCATGCCGGCACACC CGCCGGCGCTCAGGAATAA
[0162] SEQ ID NO: 6 (Formil-CoA:oxalato CoA-transferase (FCOCT) de
Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.16; Co_frc2):
ATGAACCTCCCGCTCAACGGCATCAAGATCATCGACTTCACGCACGTCC AGGCCGGCCCCGCGTGCACGCAGCTGCTGGCATGGTTCGGCGCAGACGTCAT CAAGGTGGAGCGCCCCGGTTCCGGCGACGTGACGCGCACGCAGCTGCGCGAC ATCCCGGATGTCGACGCGTTGTACTTCACCATGCTCAACTCCAACAAGCGCAGC CTGACGCTGGACACCAAGAAGCCCGAAGGCAAGAAGATCCTGGAGCAGCTGAT CCGCGAGTCGGACGTGCTGGTCGAGAACTTCGGCCCGGGTGCGCTGGACCGC ATGGGCTTCTCGTGGGAACGCATCAACGAGCTGAACCCGAAGATGATCGTGGC CTCGGTCAAGGGCTTCAGCGACGGCCATCACTATGAAGACCTGAAGGTCTATG AAAACGTGGCGCAGTGCGCGGGCGGTGCGGCGTCGACCACGGGCTTCTGGGA TGGTCCTCCCACCGTTTCCGCAGCGGCGCTCGGCGATTCGAACACCGGCATGC ACCTGGCCATCGGCATCCTGACCGCGCTGATCGGCCGCGACAAGACCGGCAA GGGCCAGAAGGTGGCCGTGTCGATGCAGGACGCCGTGCTCAACCTGTGCCGC GTCAAGCTGCGCGACCAGCAGCGCCTGGACCGCCTGGGCTTCCTGGAAGAGT ATCCGCAGTACCCGCATGGCAGCTTCAGCGACGTGGTGCCGCGCGGCGGCAA CGCTGGCGGCGGCGGCCAGCCGGGCTGGGTGCTGAAGTGCAAGGGCTGGGA AACCGACCCCAACGCCTATATCTACTTCACCATCCAGGGCCATGCCTGGGAGC CGATCTGCAAGGCGCTCGGCAAGCCGGAATGGATCGACGATCCCAACTACGCC ACCGCCAAGGCACGCCAGCCGCATATCTTCGATATCTTCAACACCATCGAGGAA TGGCTCGCCGACAAGACCAAGTACGAGGCGGTGGACATCCTGCGCAAGCATGA CATCCCGTGCTCGCCGGTGCTGTCGATGAAGGAGATCGCGGCCGATCCGTCGC TGCGCGCCAGCGGCAGCATCACCGAGGTGCCACACAAGGAGCGCGGCACGTA CCTGACGGTGGGCAGCCCGATCAAGTTCTCCGACCTCAAGCCGGAAATCACGG GTTCGCCGCTGCTGGGCGAGCATAGCGAAGAAGTGCTGGCCGGCCTGGGGTA CGGCACGGACGACATCAAGCGCCTGCGCGAGTCCCAGGTGATCTGA
[0163] SEQ ID NO: 7 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Escherichia coli MG1655; EC 2.8.3.16; Acesso UniProt P69902; Ec_frc):
ATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCA ATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAA AATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTC CTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGA GTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGA AGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTT CACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAA GGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTC AGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCT GGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCG GTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTC ACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGAC CAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAAT GGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCA GCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATAT TTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAA CCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACAT ATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGA AGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATG AAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTG GAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCT GCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCT GTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAAC CACGCCATCTGA
[0164] SEQ ID NO: 8 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Oxalobacter formigenes DSM 4420; EC 2.8.3.16; Acesso UniProt O06644; Of_frc):
ATGACTAAACCATTAGATGGAATTAATGTGCTTGACTTTACCCACGTCCA GGCAGGTCCTGCCTGTACACAGATGATGGGTTTCTTGGGCGCAAACGTCATCAA GATTGAAAGACGTGGTTCCGGAGATATGACTCGTGGATGGCTGCAGGACAAAC CAAATGTTGATTCCCTGTATTTCACGATGTTCAACTGTAACAAACGTTCGATTGA ACTGGACATGAAAACCCCGGAAGGCAAAGAGCTTCTGGAACAGATGATCAAGAA AGCCGACGTCATGGTCGAAAACTTCGGACCAGGCGCACTGGACCGTATGGGCT TTACTTGGGAATACATTCAGGAACTGAATCCACGCGTCATTCTGGCTTCCGTTAA AGGCTATGCAGAAGGCCACGCCAACGAACACCTGAAAGTTTATGAAAACGTTGC ACAGTGTTCCGGCGGTGCTGCAGCTACCACCGGTTTCTGGGATGGTCCTCCAA CCGTTTCCGGCGCTGCTCTGGGTGACTCCAACTCCGGTATGCACCTGATGATC GGTATTCTGGCCGCTCTGGAAATGCGTCACAAAACCGGCCGTGGTCAGAAAGT TGCCGTCGCTATGCAGGACGCTGTTCTGAATCTGGTTCGTATCAAACTGCGTGA CCAGCAACGTCTGGAAAGAACCGGCATTCTGGCTGAATACCCACAGGCTCAGC CTAACTTTGCCTTCGACAGAGATGGTAACCCACTGTCCTTCGACAACATCACTTC CGTTCCACGTGGTGGTAACGCAGGTGGCGGCGGCCAGCCAGGCTGGATGCTG AAATGTAAAGGTTGGGAAACCGATGCGGACTCCTACGTTTACTTCACCATCGCT GCAAACATGTGGCCACAGATCTGCGACATGATCGACAAGCCAGAATGGAAAGA CGACCCAGCCTACAACACATTCGAAGGTCGTGTTGACAAGCTGATGGACATCTT CTCCTTCATCGAAACCAAGTTCGCTGACAAGGACAAATTCGAAGTTACCGAATG GGCTGCCCAGTACGGCATTCCTTGCGGTCCGGTCATGTCCATGAAAGAACTGG CTCACGATCCTTCCCTGCAGAAAGTTGGTACCGTCGTTGAAGTTGTCGACGAAA TTCGTGGTAACCACCTGACCGTTGGCGCACCGTTCAAATTCTCCGGATTCCAGC CGGAAATTACCCGTGCTCCGCTGTTGGGCGAACATACCGACGAAGTTCTGAAA GAACTGGGTCTTGACGATGCCAAGATCAAGGAACTGCATGCAAAACAGGTAGTT TAA
[0165] SEQ ID NO: 9 (Oxalato decarboxilase (OXDC) de Bacteroides subtilis 168; EC 4.1.1.2; Acesso UniProt O34714; Bs_oxdC):
ATGAAAAAACAAAATGACATTCCGCAGCCAATTAGAGGAGACAAAGGAG CAACGGTAAAAATCCCGCGCAATATTGAAAGAGATCGGCAAAACCCTGATATGC TCGTTCCGCCTGAAACCGATCATGGCACCGTCAGCAATATGAAGTTTTCATTCTC TGATACTCATAACCGATTAGAAAAAGGCGGATATGCCCGGGAAGTGACAGTACG TGAATTGCCGATTTCAGAAAACCTTGCATCCGTAAATATGCGGCTGAAGCCAGG CGCGATTCGCGAGCTTCACTGGCATAAAGAAGCTGAATGGGCTTATATGATTTA CGGAAGTGCAAGAGTCACAATTGTAGATGAAAAAGGGCGCAGCTTTATTGACGA TGTAGGTGAAGGAGATCTTTGGTACTTCCCGTCAGGCCTGCCGCACTCCATCCA AGCGCTGGAGGAGGGAGCTGAGTTCCTGCTCGTGTTTGACGATGGATCATTCT CTGAAAACAGCACGTTCCAGCTGACAGATTGGCTGGCCCACACTCCAAAAGAAG TCATTGCTGCGAACTTCGGCGTGACAAAAGAAGAGATTTCCAATTTGCCTGGCA AAGAAAAATATATATTTGAAAACCAACTTCCTGGCAGTTTAAAAGATGATATTGTG GAAGGGCCGAATGGCGAAGTGCCTTATCCATTTACTTACCGCCTTCTTGAACAA GAGCCGATCGAATCTGAGGGAGGAAAAGTATACATTGCAGATTCGACAAACTTC AAAGTGTCTAAAACCATCGCATCAGCGCTCGTAACAGTAGAACCCGGCGCCATG AGAGAACTGCACTGGCACCCGAATACCCACGAATGGCAATACTACATCTCCGGT AAAGCTAGAATGACCGTTTTTGCATCTGACGGCCATGCCAGAACGTTTAATTACC AAGCCGGTGATGTCGGATATGTACCATTTGCAATGGGTCATTACGTTGAAAACA TCGGGGATGAACCGCTTGTCTTTTTAGAAATCTTCAAAGACGACCATTATGCTGA TGTATCTTTAAACCAATGGCTTGCCATGCTTCCTGAAACATTTGTTCAAGCGCAC CTTGACTTGGGCAAAGACTTTACTGATGTGCTTTCAAAAGAAAAGCACCCAGTA GTGAAAAAGAAATGCAGTAAATAA
[0166] SEQ ID NO: 10 (Oxalato oxidase (OXO) de Hordeum vulgare; EC
1.2.3.4; Acesso UniProt P45850; Hv_OxOx):
ATGTCCGACCCAGACCCACTCCAGGACTTCTGCGTCGCGGACCTCGAT GGCAAGGCGGTTTCGGTGAACGGGCATACGTGTAAGCCCATGTCGGAGGCCG GCGACGACTTCCTCTTCTCGTCCAAGCTGACCAAGGCCGGCAACACGTCCACC CCGAACGGCTCGGCCGTGACGGAGCTCGACGTGGCCGAGTGGCCCGGTACGA ACACGCTGGGCGTGTCCATGAACCGTGTGGACTTCGCGCCGGGGGGCACCAA CCCGCCGCACATCCACCCGCGTGCAACCGAGATCGGCATGGTGATGAAAGGTG AGCTCCTCGTTGGAATCCTCGGCAGCCTTGACTCCGGAAACAAGCTCTACTCCA GGGTGGTGCGTGCCGGAGAGACTTTCGTCATCCCGCGCGGCCTCATGCACTTC CAGTTCAACGTTGGTAAGACGGAAGCCTACATGGTTGTGTCCTTCAACAGCCAG AACCCTGGCATCGTCTTCGTGCCGCTCACACTCTTCGGCTCCGACCCTCCCATC CCCACGCCCGTGCTCACCAAGGCTCTCCGGGTGGAGGCCGGAGTCGTGGAAC TTCTCAAGTCCAAGTTCGCCGGTGGGTCTTAA
[0167] SEQ ID NO: 11 (Oxalato oxidorredutase (OOR) da subunidade alfa de Moorella thermoacetica ATCC 39073; EC 1.2.7.10; Acesso UniProt Q2RI41; Mt_OORA!):
ATGGGTAAAGTAAGGAATATCTCTGGATGTGTGGCGGTAGCACATGGTG TGCGGTTAGCCGATGTCGACGTTATCTGTTCCTATCCCATCCGGCCTTATACCG GTATCATGTCCGAGCTGGCACGGATGGTTGCCGACGGCGAGTTGGATGCCGAA TTTGTCCATGGCGAAGGTGAACACGCCCAGTTGAGTGTCGTTTACGGCGCTTCG GCTGCCGGAGCACGGGTATTCACCGGTAGTTCCGGTGTCGGCGTAACCTATGC CATGGAAGTCTATTCACCTATCTCTGGTGAACGTTTACCGGTACAGATGGCCATT GCCGACCGGACCCTGGACCCGCCCGGTGACTTTGGTGAGGAACATACCGATGC CGAGTGTTGCCGGGACCAGGGCTGGATCCAGGGTTGGGCCTCCACTCCCCAG GAAGCCCTGGATAACACCTTGATTTACTACCGGGTAGGTGAAGACCAGCGGGT CTTGTTGCCCCAGTATGCCTGCCTGGACGGGTACTTCGTCAGCCATATCCTAGG CCCGGTGGATATTCCCGACGAGGCCCAGGTGAAAGAGTTCCTGCCGCCTTACA AGAATCACCATGTCCTCGATCCCAGGAAGCCCCAGATTATCGGCCCTCAAATTG AGCCGGCCATGGGACCTCCCCTCCAATACCAGCGTTACCAGGCCGTTAAAGGC GTGCACAAGGTTCTAGAAGAGGCCTGTGACGAATTCGCCCGGATTTTCGGCCG CAAGTACGATCCCTACCTTGATGAATACCTGACTGACGATGCCGAGGTTATAAT CTTCGGCCAGGGCGCCCACATGGAGACTGCTAAAGCGGTAGCCCGGCGCCTG CGCAACCTTGGTGAAAAGGTAGGAGTAGCGCGGCTACGTACCTTCCGGCCCTT CCCGACGGAACAAATTAAAGAGCGACTTTCTAAATTCAAGGCTATTGGTGTCCT CGATGTTTCGGCCAACTTCGGCATTTCCTGCAGCGGCGGCGTCCTCCTGTCCG AACTGCGGGCCGCCCTCTATGACTACGGCGACAAAGTAAAGACCGTAGGCTTT GTAGCCGGCCTAGGCGGCGAAGTCGTCACCCACGATGAGTTCTACCGCATGTT CCAGAAACTAAAGGAGATCGCCAAGACAGGTAAAGTCGAGCAGACCTCTTATTG GATCCCCTTTGAATTATAA
[0168] SEQ ID NO: 12 (Oxalato oxidorredutase (OOR) da subunidade beta de Moorella thermoacetica ATCC 39073; EC 1.2.7.10; Acesso UniProt Q2RI42; Mt_OORB):
ATGCTGGATCGGATTGCATCTATTAAAAAGGCACCTGATGAGGAATACT ATGTTCCCGGGCATCGTACCTGTGCCGGCTGCGGCCCGGCCCTGACCTATCGC CTGGTGGCCAAAGCCGCCGGTCCCAACACCATCTTCATTGGTCCCACCGGCTG CATGTACGTGGCCAATACCAGCTACGGCTGCGGTCCCTGGCGGGTACCCTGGA TCCATGCCCAGATCACCAATGGCGGCGCCGTGGCCTCCGGTATTGAGGCCGCC TATAAGGCTATGATCCGCAAGAAGAAGACCGACGCCGAGTTCCCGAACATTATC GTTATGGCCGGTGATGGCGGCGCCGTCGATATCGGCCTCCAGGCACTGTCGG CCATGCTCTATCGCGGTCATGACGTCCTCTTTATCTGCTACGATAACGAGTCCTA TGCCAATACCGGTATTCAGACCTCCCCGACGACGCCCTACGGGGCTAATACGA CTTTTACCCCGCCTGGTGAGGTGGTACCGGAGGGCAAGAAGCTCTTCCCGAAG GATAACCCCAAGGTTATCGCCCACGGTCACCCGGAACTGAAGTATGTCGCCAC GGCCTCCATCGGCTGGCCGGTAGACCTGATGAACAAGGTCCGTAAGGGCTTAA ACCAGGAAGGTCCGGCCTATATCCACATCCACGCTCCTTGCCCCAAGGGCTGG CAGTTCCCGGCCGACAAGACCATTGAAATGGCCAAATTGGCCGTCCAGACGGG CATGTTCCAGCTCTACGAATATGAGAATGGTGAGTATAAACTGTCGGTTAAAGTC GATAAACGGAAACCGGTCAGCGAGTACATGAAACTCCAGAAGCGGTTTGCTCAC CTGAAGCCCGAGCATATCGCCAAGATGCAGGCCTTTGTCGACGCCCGCTGCGC TGAAGTAGGTATCACCGTGCCGGTAGTCGCTTCCAACGCTTAA
[0169] SEQ ID NO: 13 (Oxalato oxidorredutase (OOR) da subunidade delta de Moorella thermoacetica ATCC 39073; EC 1.2.7.10; Acesso UniProt Q2RI40; Mt_OORD):
ATGTCCACCAAAGATCTCTTTGCTGAACCCAATCTCAAGCAGATTACTGT GTGGGCCCGGGGCGTTGTCATGAACAAGGACGCCCGTGATATCGTGGTGGCC CTGACAGAAGCGGCTGCCAAAGAGGGTAAATATGTTCAGGCCTGGGAGAACTA TGTTGACCTCCCCGACCGGATCTACGTCCCGGTAAGGGCCTATGCCCGCATCA GCAGCGATCCCATTGAAAGCAAATACATTTACGAGAATGAGACTCCCGACATTG TTGTTTTAGTTGAAGAATCCCTGATCAAAGGAGTGCCCATACTGAAGGGCATCC GTCCGGGTAGTACCCTGGTTGTAAATACCAAACGTTCCATAGATACTATCCTTGA GTTCCTGGGTGATACCGGCAATCTGGCCCAAATCGTAACGGTGGATGCCAACA GTATGGCCGAGGCTGTCATGACTTTGTCTGGCGCTGAAGGCGCTACTGACGCT ACGGGTATTGGCGCCGGCATAGCGGCTCCCATTGCGGGTGCTGTTGTCAAGGC TACAGGTATAGTTGATGTTGAAAACCTCGCCGCTGTGGTCAAGAATCCCGCCGC CATGCGCCGGGGCTATGCGGAAGCCCAGGTGCGCCAGCTGCCGCCCCATGAA GCAGTTGAGGAAGCGGCCGTATCAGCGACTGAATTGTTGAGGCAAATGCCCTTT GCCGGGACGGTTCCCTCACCGGTAACGGAGAATGAGGGCATGGTAACCGGCA ACTGGCGCATCCAAAGGCCCATTATCGACCGGGAAGCCTGTACCGAATGTTATA CCTGCTGGATCTACTGCCCCGATTCCTGCATCACCAGGACCGAAGAAGGACCG GTATTTAATATGAAGTACTGCAAGGGCTGCGGCCTCTGTACGGCGGTCTGCCCC AGCGGCGCCCTGACTAATGTACCGGAACTTGACTTCAAAGATTAA
[0170] SEQ ID NO: 14 (Oxalato--CoA ligase / Oxalil-CoA sintetase (OXS) de Arabidopsis thaliana col-1; EC 6.2.1.8; Acesso UniProt Q9SMT7; At_AAE3):
ATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATT CCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCG TCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAAT CAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTAT AATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAG CGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATT GTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAA AATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATC CGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCC GGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGG GTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGT GTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTT CATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACT CTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAG TATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACC GCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTT GCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAG CACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAA ACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTA GGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAAC AAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAAC CCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATC GGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTT ATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTA ACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATAT GGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAA GAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAG AGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGC CGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGA
[0171] SEQ ID NO: 15 (Oxalato--CoA ligase / Oxalil-CoA sintetase (OXS) de
Methylobacterium extorquens AM1 / DSM 1338; EC 6.2.1.8; Acesso UniProt C5APP0; Me_oxs):
ATGACTATGTTATTACCTGAACCATCAGCTTTGTTACCAACAGATTCAAA GGAAGCCTCAGTCCCAGCAACAACATTGCATGAGTTGATACAAGCTGGTGCAGA CGCTGCCCCTGCCTTATCTTCTCCAGGAGGAGTACCATTAACCTTTCAAGCATTA AGAGCCTTAACCGAGAGAACCGTCGCCGACTTGAACGCAAGGGGTATTGGTAG AGGTGATAGAGTTGCCATTGTTTTGCCAAATGGACCAGAAATGGCTGCAGCTTT CATTGCAGTAGCAGCCGGTACAACTTCAGCACCTTTAAATCCTTCTTACAAGGC GGACGAATTTGAGTTCTATATGTCAGATTTAGGTGCAAAATTATTATTAGTCGCA GAGGGTTCTGAGACTCCTGCCGTTGCCGTGGCTGAGAAATTGGGTGTTTCAGTA GCTAGATTAAGACCAACTCCAGATGAAGGAGCAGGTTCTTTTACTTTACATTTTG CCTCAGAGTCTACTGGTCCAACCGAAAAGTCAGGTCCTGCAGGTTCAGATGATA TAGCTTTGGTATTGCACACATCAGGTACCACATCTAGGCCTAAGATTGTCCCATT GACTCAAGCAAATGTATGCGCATCTGCTAGAAACATTAGGACTGCATTAGCCTTT GGCCCGGAAGATAGAGGATTAAACATAATGCCTTTGTTCCATATACACGGATTAA TCGCTGGAATTTTAGCCCCATTATCTGTCGGTGGTCAAGTCTCTTGCACTCCAG GTTTCAACGCATTGAAATTCTTTGGATGGATGGATGAAGTCAATCCAACCTGGTA CACCGGAGTACCAACAATGCATCAAGCCATTTTAGGAAGGGCAGCAAGAAATAA AGAAATTATCGCCAGGAACCCTTTAAGATTTATCAGATCTTCTTCATCATCTTTGC CACCACAAGTCATGAAAGAGTTGGAGGAAACCTTCGGTGCTCCTGTTATTGAAG CATACGGAATGACCGAAGCTGCACATCAAATGGCCTCAAACCCATTACCTCCTA AGCCTCACTATGCTGGTTCTGTAGGTTTGGCTGCAGGTCCTGAGATTGCTGTTG TCGACTTAGACGGTGAACCTTTACCAGCTGGAGAGACAGGTGAAATAGTCATAA GAGGTGACAACGTTATGAAGGGTTACGAAAACAACGAGAAGGCAAATGCAGAA GCATTCACCAAGCAGGGTTGGTTCAGAACTGGTGACCAAGGTGTCTTGTCTCCA GAGGGATACTTATCTATCACTGGAAGGTTAAAGGAGATAATCAATAGGGGTGGT GAGAAAATCTCACCAAGAGAAGTAGATGAAATTTTGATGGACCATCCTGCAGTAT CACAATGCGTTACATTTGCGGTTCCTCATGATAAATTGGGTGAAGATGTTGCTGC TGCCATCGTCTTAAGGGAGGGTGTTGAAGCCGTTGAAAAGGACATTCGTAGCTT CGCTTCAGAAAGATTAGCAGCATTTAAAGTACCTGCCAAAATCTTAATTTTGGAT GAAATCCCAAAAGGTGCCACAGGTAAGTTGCAAAGAATAGGTTTGGCACAGAAA TTGGGATTGGTTTAA
[0172] SEQ ID NO: 16 (Oxalato--CoA ligase / Oxalil-CoA sintetase (OXS) de Saccharomyces cerevisiae S288C; EC 6.2.1.8; Acesso UniProt P38137; Sc_YBR222C):
ATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGT ATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAG GGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCT CCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAAT GGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGC GCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACC TGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTG AAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGA TGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAA AAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTT AAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGT GGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAA GCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGT CGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTA GAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGG ACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCAT GATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGA TCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTC AATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACC TCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACA AGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAA AGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAA TCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGG TGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAA AGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCAT TATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGA TATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAAT GACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATT CCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCC AGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGT TGTAA
[0173] SEQ ID NO: 17 (Oxalato:formiato antiportadora (oxlT) de Bifidobacterium animalis subsp. lactis DSM 10140; Acesso UniProt B9X293; Ba_OxlT):
ATGACAAAGATATTGATAGACGACATCATCCCCATCATCGTCATCATGG CGTTGGGCTATGTCTGTGGAAAACTCAGCTACTTCGACAACGATCAGCGTCAGG GCCTCAACAAGCTCGTGCTGAACATCGCCCTCCCTGCCGCACTGTTCATCTCGA TCGTCAAGGCGACCCGTGAGATGCTCGCACAGGATGCGGTGCTCACAATTCTC GGCTTCATCGGCATCATCGTGATGTTCATGTTGAGCTACTACCTGTGCCGACTG ATGTTCCACCATTCGATCCAGGAAGCGGCCGTATGCGCGTTGATCGCCGGCTC GCCGACCATCGGCTTCCTCGGCTTCGCCGTGCTCGACCCGATCTACGGCGACA CCGTGAGCACGAACCTCGTCATCGCGATCATCTCGATCGTCGTCAACGCCGTC ACGATTCCGATCGGCATGTATCTGATCAACCTCGGCCAGAGCAAGGATCGCGA GCGCCTGTCGAAGGCCGCCGTCACGAACTCGAAGGGCCAGGTCAGCATCGCC AATCCGAAGGACGATATCGCCGTCGATCCGAACAAGGATGCGAAGACCGACAA GACGGCAGAGGTGATGATCTCCAAGTCGTCGAACATGGGCAAGAAGAAGAACC AGAACCTCGAAGCGCTCATCAACGCACTCAAGCAGCCTGTGTGCTGGGCTCCG CTGCTCGCCATCGTGCTCGTGCTCATCGGCGTGCGCGTGCCGTCCGGTTTCGC TCCCACCTTCGACCTGATTGCCAAAGCGAACTCCGGTGTCGCCGTGCTGGCCG CCGGCCTCGCGCTCTCCACTGTGAAGTTCTCGCTGGGCTGGGAAACGATCTGG AACACGTTCTACCGCTTGATTCTCACCCCTGCGGCATTCCTCGGCGTCGGCCTG CTGCTGGGCATGGGCAGCAATGTGAACAAGCTCTCCATGCTCGTCATGGCTGT GGCCCTGCCGCCTGCATTCTCCGGCATCATCATCTCGAGCCGCTACAACATCTA CGTCAAGGAAGGTGCGTCGACCACCGCGGTTTCCACGGTTGCGTTCGCCGTCA CCTGCCTGCTGTGGATCTGGCTCGTGCCGCTGTGCTGCCACTGA
[0174] SEQ ID NO: 18 (Oxalato:formiato antiportadora (oxlT) de Cupriavidus oxalaticus ATCC 11883; Co_OxlT1):
ATGAGTAACGCAACGGCAGGGGCCCTGAGCCCCAATCAGGGAGCTTCG GAATCCACGCGCTGGATCCAGCTGCTCGTCGGCGTGGTCTGCATGATCGCCAC GGCCAACATCCAGTACGCCTGGACGCTGTTCGTGCCTGAGATCCAGGATACCT ACGGCTGGTCACGCGCCAGCATCCAGATTGCCTTTACCGTGTTCGTGCTGGTG CAGACCTGGCTGGCGCCGATCGAGGGCTACTTCATCGACAAGTTCGGGCCGCG CATGATGGTGGCCTTCGGCGCGCTCTTTATCGGCACGGCGTGGGTCATCAACT CGCAGGCGACCACGCTGATGGGCTTCTACGTCGGCGCCGCCGTCGGCGGCCT GGGCGTGGGCTCCATCTACGCGACCTGCATCAACAACGCGCTGAAGTGGTTCC CGGACCGCCGCGGCCTCGCGGTCGGCCTGACCGCCGGCGGCTATGGCGCGG GCTCGGCGGCGACCATCCTGCCGATCGCGGCGATGATCGAGTCGCAGGGTTT CCAGCACACCTTCCTGTTCTTCGGCCTGCTGCAGGGGTCGCTGGCCTTTGTCG CGGCGTGGTTCCTGCGCTCGCCCAAGACCGGCGAGGTGCGGGGGTCGAAGAA GCTGGCCCAGGCCACGCGCGACTACACCCTCAAGGAAGCGCTGTGCACCAGG CTGTTCTGGCTGATGCTGGTGATGTTCGTGCTGGTGGTCACGGGCGGCATGAT GGCCGTGGCGCAGCTCGGTGTCATCGCCAAGGACCTTGGCGTGAAGGAATTCC AGGTCGACCTGCACTTCTTCGTGATGGCTGCACTGCCGCTCGCCCTGATGCTG GACCGCATCATGAACGGCATCTCGCGTCCGCTGTTCGGCTGGATCTCGGACAA CATCGGCCGTGAAAAGACCATGGTGATCGCCTTTACGCTGGAAGGGCTGGGCA TCATCGCGCTGGGCTACTTCGGCAGCAACCCGTACGCCTTCCTGATCCTGTCC GGCGTGGTGTTCCTGGCCTGGGGCGAAGTCTACTCGCTGTTCTCGGCACTGGC GGGCGATGCCTTCGGCACCAAGCACATCGGCAAGATCTACGGCGTGCTGTACA CCGCCAAGGGCATCGGCGCACTGTTCGTGCCGATCGGCAACCTGCTGATGGAA GCCACCGGCACGTGGTCGACGGTGCTCTACACGGTGGCGGTGATGGACCTGA CCGCGGCGTTCCTTGCCATCATGGTGCTGCGGCCGGTGCTTGCCTCGCACGTT GCGACATCGAGGCAGCGGTTTGCGCAGGAATCTGCCGCGGCCGGCGCGCAAG TGGCGGCTTGA
[0175] SEQ ID NO: 19 (Oxalato:formiato antiportadora (oxlT) de Cupriavidus oxalaticus ATCC 11883; Co_OxlT2):
ATGGAATTGCAGCAGAGGGAGTCCACGTCGCGCTTTGCGTCGCCGTGG GTGCAATTGGTCTTTGGCGTGATCTGCATGGCGATGATCGCCAACATGCAGTAC GGCTGGACGCTGTTCGTCAACCCGATCGACGACAAATACCACTGGGGCCGCAC CGCGATCCAGGTGGCATTCACCATCTTCGTCGTGACGGAAACCTGGCTGGTAC CGATCGAGGGCTACCTCGTCGACAAGTACGGCCCGCGCCCGGTAGTGGTGGG CGGCGGGTTGCTGTGCGCCATCGCGTGGGCGCTGAACTCGGTGGCGTCCTCG CTGCCGATGCTGTACTTCGCGGCCGCAATCGGCGGCGTGGGTGCCGGCGCGG TCTACGGCACCTGCGTGGGCAATGCGCTCAAGTGGTTCCCCAACCGGCGCGG GCTGGCGGCGGGCATTACCGCAGCAGGCTTTGGCGCTGGTTCCGCGATGACC GTGGTGCCGATCGCCAACATGATCAAGACCTCGGGCTATGAAGCCACCTTCCT GTGGTTCGGGCTGGGGCAGGGCCTGATCGTCTTCATCCTCGGCCTGGCCCTGT ATCCGCCGTCGGCCAGGATCCTGAGCGAGGTCAAGACCACGCTCAAGGCCGC CGCGACCTACAACGCCACGCCGCGCCAGGTGCTGAAGTCGCCGATCTTCTGGA TCATGTACGCCATGTTCGTGATGATGGCCGCCGGCGGGCTGATGGCCACCGCG CAGTTGGGCCCGATCGCCAAGGACTTCGGCCTGCATGACTCGCCGGTGTCGAT CCTCGGCCTGACGCTGCCCGCGCTGACCTTCGCGCTGACCATCGACCGCGTG CTCAACGGCCTGACCCGCCCGTTCTTCGGCTGGATCTCCGACCATATCGGCCG CGAGCGCACCATGTTCTTCGCCTTCGCGGTGGAGGCGGTCGGCATCCTGCTGC TGTCCAAGTACGGCCACAACCCGGTGGCGTTCGTGGTGCTGACCGGCATCGTG TTCTTTGCCTGGGGTGAGATCTACAGCCTGTTCCCGGCCACCTGCGGCGATAC CTTCGGTCCCAAGTTCGCCGCCACCAACGCCGGCTTGCTGTACACCGCCAAGG GCACGGCTGCGTTGCTGGTGCCGTTCTCCAGCGTGATCACCGCCGCCACCGG CGACTGGCACGCGGTGTTCATGCTCGCCTCCGGCATGGCGGCGCTGTCGGCG GTGCTGGCGCTGTTCGTGCTCAAGCCGATGCGTGAAGCCCATGCCCGCAAGTA TGTCCACGCCAATACCGCGGCGCCGATGGGCTATCGTGCCGTGCAGGAAGACC TGACCTGA
[0176] SEQ ID NO: 20 (Oxalato:formiato antiportadora (oxlT) de Escherichia coli MG1655; Acesso UniProt P37662; Ec_YhjX):
ATGACACCTTCAAATTATCAGCGTACCCGCTGGCTGACACTCATCGGTA CTATCATTACCCAGTTTGCGCTGGGGTCGGTTTATACCTGGAGCCTGTTTAATG GCGCGCTTTCCGCCAAGCTGGATGCGCCGGTAAGCCAGGTCGCTTTCTCTTTC GGCTTGTTAAGTCTGGGGCTGGCAATTTCGTCTTCTGTTGCGGGCAAATTACAG GAACGTTTTGGCGTTAAACGCGTCACCATGGCTTCCGGCATTTTGCTGGGATTA GGCTTCTTCCTGACAGCGCATTCTGACAACCTGATGATGCTGTGGTTAAGCGCC GGTGTGCTGGTGGGACTGGCAGATGGCGCGGGTTATCTGCTGACGCTCTCTAA CTGTGTGAAGTGGTTCCCGGAGCGTAAAGGTCTGATCTCCGCGTTCGCTATCG GTTCTTATGGTCTGGGTAGCCTGGGTTTCAAATTTATCGACACGCAGCTGCTGG AAACGGTCGGTCTGGAAAAAACCTTTGTGATTTGGGGAGCGATTGCGCTGTTGA TGATTGTTTTCGGCGCAACGTTAATGAAAGACGCACCAAAACAGGAAGTGAAAA CCAGCAATGGTGTGGTGGAGAAAGATTACACGCTGGCAGAGTCGATGCGTAAA CCGCAGTACTGGATGTTAGCGGTAATGTTCCTGACCGCCTGCATGAGCGGCCT GTACGTGATTGGGGTAGCGAAAGATATCGCCCAAAGTCTGGCACACCTTGATGT GGTTTCCGCAGCCAATGCAGTCACTGTTATTTCCATCGCCAACCTTTCAGGTCG TCTGGTGCTGGGTATTCTGTCTGACAAAATCGCCCGTATCCGTGTTATTACCATT GGTCAGGTGATATCGCTGGTGGGTATGGCGGCCCTGCTGTTTGCACCATTGAA TGCAGTGACGTTCTTTGCAGCGATTGCCTGCGTGGCATTTAACTTTGGCGGCAC TATTACCGTCTTTCCGTCACTGGTCAGTGAGTTCTTTGGCCTCAATAACCTGGCG AAAAACTACGGTGTGATTTATCTCGGTTTCGGTATCGGTAGCATTTGTGGTTCGA TTATCGCCTCACTGTTTGGCGGCTTCTATGTGACCTTCTACGTGATTTTCGCCCT GCTGATTCTGTCATTGGCGCTTTCTACGACGATTCGTCAGCCAGAGCAGAAAAT GTTGCGTGAGGCGCATGGCTCCCTTTAA
[0177] SEQ ID NO: 21 (Oxalato:formiato antiportadora (oxlT) de Oxalobacter formigenes DSM 4420; Acesso UniProt Q51330; Of_oxlT):
ATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTT GGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATA TTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGC TGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCC TGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGT GGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCT GCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGT ATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTC CGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGAT CAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTT GATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAG CAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAA ATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACT TTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGT CTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGT GGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACC ATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTG CTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACAT GGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGG CATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGA TCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCG CTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTT ATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAA AAAGCTGTACATTAG
[0178] SEQ ID NO: 22 (Oxalil-CoA decarboxilase (OXC) de Bifidobacterium animalis subsp. lactis DSM 10140; EC 4.1.1.8; Acesso UniProt B9X297; Ba_oxc):
ATGGTTGATGTAAGTGTAACTGCTACAAGCTCAGACCAGAATCTCACGG ATTCTCCCCACTACCTCGCCGAAACGCTCATCAAGAACGGTGTCAAGCATATGT ACGGCGTCGTCGGAATCCCGGTCACTGATTTCGCACGTATCGCACAGGGCATG GGCATCCGCTTCATCGGCATGCGCCATGAGGAGGACGCGGTGAACGCTGCCG CTGCCGAAGGATTCCTCACCGGTCGCCCAGCTGTGGCGCTCACCGTTTCCGCG CCGGGCTTCCTCAATGGTCTGGCACCGCTGCTTGAAGCCACCACGAACGGCTT CCCGGTCATCATGATCGGCGGTTCGTCCACTCGCCATGTCGTCGACATGCACG AAGGCGAATACGAAGGCCTCGACCAAATGAACTATGCGAAGCAGTTCTGCAAG GAATCGTTCCGCATCGACAAGATCGAAGACATTCCGCTTGCTGTGGCCCGCGC CATGCACATCGCATGCTCCGGCCGTCCGGGCGGTGTCTACATCGATTTCCCGG ACGACGCCGTCGCCCAGACGCTCGACAAGGATGTCGCCGAGTCGCAGCTGTG GGTCGCGAACCAGCCGGCTCCGGCAATGCCGCCGGCGCAGTCCTCTGTGGAT GAAGCGCTCAAGCTGCTCTCCGAGGCCAAGAACCCTCTCATGCTTGTGGGCAA GGGTGCGGCGCTGGCCCAGGCCGAGGACGAACTGCGTGAATTCGTCGAGAAG ACCGACATGCCATTCCAGCCGATGTCGATGGCCAAGGGCGTCATTCCGGACGA TGACCCACACTGCACGGCGAGCTGCCGCGGACTCGCGCTGCGCACCGCCGAC GTCGTGCTGCTCGTCGGCGCTCGTCTGAACTGGATGCTCAATTTCGGCGAGGG CAAGGAATGGAACCCGAACGTCAAGTTCATCCAGATCGATATCGACCCGAACGA GATCGAGAACGCCCGTTCCATCGCATGCCCGGTGGTCGGCGACATCAAGTCCG CCATGCAGATGATCAATGCCGGACTCGAGAAGACGCCAGTGAAGGCGTCCGCG CAGTGGCTCGACATGCTCAAGGCCGACGCCGAGAAGAACGATGCCAAGTTCGC CGCCCGCGTGAACTCGAACACCGTGCCGATGGGTCACTACGACGCGCTCGGC GCCATCAAGAAGGTGTACGACCAGCACAAGGACATGATCCTGACCAACGAGGG CGCGAACACGCTCGACGATTGCCGCAACATCATCGACATCTACCAGCCGCGCC ATCGTCTAGACTGCGGCACGTGGGGCGTTATGGGCTGCGCGGTCGGCTATTCA ATCGGCGCCGCAGTGGCCACCGGCAAGCCTGTCCTGTACGTCGGCGGTGATTC CGGCTTCGGCTTTGACGGCATGGAAGTCGAGGTCGCCTGCCGCTACAATCTGC CGATCACCTTCGTCGTGCTCAACAACGGCGGCATCTACCGCGGCGATTTCGAG AATCTCGGCGACGACGGCGACCCGTCGCCGCTGACGCTGAGCTACGACGCCC ACTACGAGCGCATGATCGAGGCGTTCGGCGGCAACGGCTATTACGCGACCACC CCGGCGGAAGTCGAGCAGATGGTCGGCGAGGCCGTCGCCTCCGGCAAGCCGA GCCTCGTGCACGTGCAGCTCGCCGATTATGCGGGCAAGGAGTCCGGGCACATC TCCAACCTGAACCCGAAGCCCGTCGTCGGCCCGCTCGCCACTTCCGAAATGAC CGCGAACCCCTACCTCAAGGGCGCCCATATGTGA
[0179] SEQ ID NO: 23 (Oxalil-CoA decarboxilase (OXC) de Cupriavidus oxalaticus ATCC 11883; EC 4.1.1.8; Co_oxc):
ATGGCAGAAGTCGGAAACGAGCTGCAGCGTCACGCCGCGGAAGAGCA TCAGGCACAAACCGATGGTTTTCATCTGGTCATCGACGCGCTGAAGCTGAACGG CATCGAAAACATCTACGGCCTGCCCGGCATTCCGGTCACCGACCTGGCTCGGC TGGCGCAGGCAAACGGCATGCGCGTCATCAGCTTCCGCCACGAGCAGAACGCA GGCAACGCCGCAGCGATTGCCGGCTTTCTGACGCAAAAGCCAGGCGTTTGCCT GACCGTGTCTGCGCCAGGGTTCCTGAACGGGCTCACGGCGCTGGCGCATGCG ACCACCAACTGCTTCCCGATGATCCTGATCAGCGGCTCGAGCGAGCGCGAGAT CGTCGATCTGCAGCAGGGTGACTACGAAGAGATGGACCAGCTGGCCATCGCCC GTCCGCACGCCAAGGCTGCCTTCCGCGTGCTGCACGCCGAAGACATCGGCGT CGGGATCGCGCGTGCCATCCGTGCCGCCGTGTCGGGCCGCCCGGGCGGTGT GTACCTGGACCTGCCGGCCAAGCTGCTGGGCCAGTCGATGGAAGCCGAGAAG GGCCGCAAGTCGCTGATCAAGGTGGTGGATCCGGCCCCGCGCCAGCTGCCTG CTCCGGACTCGGTCGACCGTGCCGTCGCGCTGCTGAAGAGCGCCAAGCGCCC GCTGATCCTGGTAGGCAAGGGTGCTGCGTACGCACGCGCCGAAGCGGATATCC GCACGCTGGTCGAGAAGACGGGCATTCCGTACCTGCCGATGTCGATGGCCAAG GGCCTGTTGCCCGATACGCACCCGCAATCGGCCTCCGCCGCACGCTCGTATGT GCTGGCCGAAGCCGACGTGGTGCTGCTGGTCGGCGCCCGCCTGAACTGGCTG CTGTCGCACGGCAAGGGCAAGACCTGGGGCAAGCCGAAGCAATTCATCCAGAT CGACATCGCGCCGACCGAGATGGACAGCAACGTCGCCATCGCCGCGCCGGTC GTGGGTGACATCGGTTCGTGCGTGTCGGCGATCCTCGACAAGGTCGGCGACGA CTTTGCCAGGCCGGGCGCCGACTGGCTGAACGCGGTGGCTGACCGCAGGGAC ACCAACCTGGCGAAGATGGCCGAAACCCTGGCCAGGTCCAGGGACGCCTCGC CGATGAACTTCCACGGTGCGCTCGGCGTGCTGAAGGATGTGGTCAAGGCCAAC CCGAACATCTCGTTCGTCAACGAAGGCGCCAACACGCTGGACTACGCCCGTGC CGTGATCGACATGTACGAGCCGCGCAAGCGCCTGGACGTAGGCACCTGGGGC GTGATGGGCGTGGGCATGGGCTACGCGGTCGCCGCCGCGGTCGAAACCGGCA AGCCGGTGCTGGCGCTGTGCGGTGACAGTGCGTTCGGCTTCTCGGGCATGGA AGTCGAAACGATCTGCCGCTACAACCTGCCGGTCTGCATCGTCATTTTCAACAA CAACGGCGTCTACAAGGGGATCGACGTGAATCCGACCGGCGGCAGGGATCCG GCGGTCACGACGTTCGTCCCGGGCGCGCGCTACGACAAGATGATGGAAGCGTT CGGTGGTGTCGGTGCCAATGTCACCACGCCGGCGGAACTCGAAGCCGCGGTA AACGAAGCGCTGCGCTCGGGCAAGCCCACGCTGGTCAACGCCGTCATCGACC CGGCTGCCGGCACCGAAAGCGGTCGCCTGACCAATCTGAATCCGCAAAGCTCG GCCAAGAAGTAA
[0180] SEQ ID NO: 24 (Oxalil-CoA decarboxilase (OXC) de Escherichia coli
MG1655; EC 4.1.1.8; Acesso UniProt P0AFI0; Ec_oxc):
ATGTCAGATCAACTTCAAATGACAGATGGTATGCATATCATCGTTGAAGC ATTAAAACAGAATAATATTGACACTATTTATGGTGTTGTAGGTATTCCTGTGACG GATATGGCACGCCATGCCCAGGCGGAAGGCATTCGTTATATTGGTTTTCGTCAT GAGCAGTCGGCAGGCTATGCCGCTGCGGCAAGCGGTTTTCTTACCCAAAAACC GGGGATCTGCCTGACAGTTTCTGCGCCAGGATTCCTCAATGGTTTGACCGCATT GGCCAACGCAACGGTAAATGGTTTTCCGATGATCATGATTAGCGGCTCCAGCGA CCGCGCGATCGTCGACCTACAGCAAGGTGATTATGAAGAGCTGGACCAAATGA ATGCGGCAAAACCGTATGCCAAAGCAGCATTTCGCGTTAATCAGCCGCAGGATC TTGGCATTGCATTGGCACGCGCTATCCGGGTTTCTGTATCGGGTCGCCCTGGC GGAGTTTATCTTGATTTGCCAGCAAATGTCCTGGCCGCGACGATGGAAAAAGAC GAAGCGTTAACCACGATTGTTAAAGTTGAAAATCCGTCGCCAGCATTATTGCCAT GCCCGAAGTCAGTCACTAGCGCAATTTCGCTTTTAGCAAAAGCTGAACGGCCAT TAATTATCCTTGGCAAAGGCGCGGCGTATTCACAAGCTGATGAACAGCTTCGTG AATTTATTGAAAGTGCTCAGATTCCATTCCTGCCAATGTCTATGGCGAAAGGGAT CCTTGAAGATACGCATCCACTTTCTGCGGCAGCTGCGCGTTCGTTTGCCCTGGC AAATGCTGACGTTGTCATGCTTGTTGGTGCACGACTGAATTGGTTATTGGCACA CGGTAAAAAAGGATGGGCGGCAGATACACAGTTTATTCAACTGGATATTGAACC GCAGGAAATTGACAGCAACCGCCCCATTGCTGTGCCAGTCGTTGGTGATATTGC ATCCAGTATGCAAGGTATGCTGGCAGAACTGAAACAAAACACATTTACGACTCC ACTGGTATGGCGCGATATTTTAAATATCCACAAGCAGCAAAATGCACAAAAAATG CATGAAAAATTAAGTACAGATACTCAACCATTAAATTACTTTAATGCATTAAGTGC TGTGCGCGACGTATTGCGCGAGAACCAGGATATTTATTTAGTTAATGAAGGTGC AAATACCCTGGATAATGCACGAAATATTATTGATATGTATAAACCACGTCGTCGT CTGGATTGTGGTACCTGGGGTGTCATGGGCATCGGTATGGGCTATGCCATCGG TGCTAGCGTGACTTCTGGTTCTCCGGTTGTCGCCATTGAAGGTGATAGTGCTTT TGGTTTCAGTGGGATGGAAATTGAAACGATTTGTCGATATAACCTGCCGGTGAC GATCGTTATTTTTAATAATGGCGGCATCTACAGAGGAGATGGTGTTGATCTCAGT GGCGCTGGTGCACCATCACCAACGGATCTGTTGCACCATGCAAGGTATGACAA ATTAATGGATGCGTTTCGTGGCGTTGGCTATAACGTCACCACGACAGATGAACT TCGTCATGCTTTAACCACCGGTATTCAGTCGCGCAAACCGACCATTATTAATGTG GTCATCGACCCTGCAGCAGGAACTGAAAGTGGCCATATTACCAAACTTAACCCA AAACAAGTCGCTGGTAATTAA
[0181] SEQ ID NO: 25 (Oxalil-CoA decarboxilase (OXC) de Oxalobacter formigenes DSM 4420; EC 4.1.1.8; Acesso UniProt P40149; Of_oxc):
ATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGAT CGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCC TATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTT CCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGG AAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGAC TTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTC CAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCA GATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAA GACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCA GGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTA GAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATT CCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGT CCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATC CGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAA GGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGC ACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGAT GCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGA TCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTG TTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTC CAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAAC AAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAA CTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATAT TTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGA CATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTAT TGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCG CTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCT GCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAA AGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCG TGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAA TACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACC ATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTAT CAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAG
[0182] SEQ ID NO: 26 (Oxalil-CoA decarboxilase (OXC) de Saccharomyces cerevisiae S288C; EC 4.1.1.8; Acesso UniProt P39994; Sc_YEL020C):
ATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATG GCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATA CGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCT CATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTAT TGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAG TAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACAT AAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGT TTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAA TTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTT ATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCG ATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCG TACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGT GCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCAT TTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACG TTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGG GGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAA TCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCT CACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTT TAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGC AAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAA GACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCT TATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATT GCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGAC CAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCA TCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGC CATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGAT GAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCC AACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTG CCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGC CGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGA GCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATG A
[0183] SEQ ID NO: 27 (Oxalil-CoA redutase / Glioxilato:NADP+ oxidorredutase (OXR) de Methylobacterium extorquens AM1 / DSM 1338; EC
1.2.1.17; Acesso UniProt C5APN9; Me_PanE):
ATGTCAATTGCCATTGTTGGTGCCGGTGCAATTGGAGGATATTTAGGTG TCAGATTAGCTGAAGCTGGTGAGGATGTTACATTCATAGCTAGATCTAATGCTGC AGCCATACAAGCAGATGGAATGAGATTGATTGAAGAGGATGGGACCGAAATTCA CTCTAAATCTGTCAAAGCCACTCGTTCAATGCAAGAAGCAGGAGTTCACGAGGT TGTTTTATTAACAGTTAAGGCACACCAAGTTGGTCCTATAGCTGCCGATTTACAT CACTTGATAGGTCCAGACACCGTAGTCGTCACTATGCAGAATGGTATTCCTTGG TGGTACTTTCTAGGTGGATACTCTGGAGATCATGCAGGAACAAGGTTGGAATCA GCTGATCCAGGAGGTTTAATTGCTGATCATTTAGACCCTAAGCATGTCATTGGTT CTGTTGTTTACCCTGCAACTGTATTGACAGACCCAGGTACTGTTAAAGTTATCGA GGGTAATAGGTTTGGTTTGGGAGAATTGGATGGTTCAAAATCTGAAAGAGTTTTA GCCTTATCTCAGAGGTTAGCAAGGGCCGGTTTTAGAGCCCCAGTCACTTCAGAC ATTAGAGCAGAGATCTGGTTGAAGTTGTGGGGAAATTTATCTTTCAATCCCATTA GCGCCTTAACACATGCAACCTTGGAGGACATCTGTAGATTTCCTGATACTAGGG CTATCGCCGCAGAGATGATGAGAGAGGCCGAAGTCATCGCCAATAAATTGGGT GTAACATTTAGATTAGGAATAGATAAGAGAATAGCTGGAGCTGAAAAGGTCGGT CCACACAAGACCTCTATGTTGCAAGACGTAGAAGCTGGTAGACCAATTGAGTTG GAAGCCTTAGTCGGATCTGTAATCGAATTAGGAAGGTTAACTGGTACTCCTACT CCACATATTGATACAGTCTTTGCTTTGATGAGATTATTGGCCCAATCTTTAGAAA GAGCACAAGGTAGATTGGCTATTCAGGGAGCATAA
[0184] SEQ ID NO: 28 (Succinil Putativo-CoA:oxalato CoA-transferase (SCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.2; Co_ACOCT):
ATGACGCAATCCAACCTGCCCGATTTGCCCGACCTGCCGCTGACCGGC CTGCGCGTGATCGATTTCTCGCGCGTGCTGGCCGGCCCCTATTGCACCGCGCT GCTCGGCGACCTCGGCGCCGAGGTGATCAAGGTCGAGCCGCCCGGCGGCGAC GACTATCGCGCGGTGGGTCCCTTCGCCGGCGGCAAGAGCGGCCTGTTCTGCG CGATGAACCGCAACAAGCAGAGCATCGTGATCGACCTGAAGACGGAGGACGGC CTGGCTGTCGCCCGCGCGCTGTGCCGCGGTGCCGACGTGGTGGTGGAAAACT TCCGCCCCGGCGTGGCCGACAAGCTTGGCATCGGCTACGCCGCGCTGCGCGA ACTGAACCCCTCGCTGGTCTATGCCAGCGTGTCGGGCTTCGGCCAGACCGGCC CGGAATCGCACCGCCCGGCCTACGACATCATCCTGCAGGCGATGTGCGGGCT GATGGACGCCACCGGCGCGCCCGACGGCGCGCCGACCATGCTTGGCGAAGCC GTGTCCGATGCGGTCAGCGGCCTGTTCGCCTCGTGGGGCGTGCTGGCCGCGC TGCTGGCGCGCGAGAAGACAGGGCGCGGCACGCATGTCGATGTGTCGATGTT CGATGCCACGCTCAGCCTCAGCGCCACGCTGGTCGCGCGCTACGCGGCCACC GGGCTGGCGCCGCGGCGCGTGGGCAACCGCCACCCGTCGTCGGCGCCGTTT GGCGCCTACCGCGCCGCGGATGGCTTCTACGTGGTCGCGGTGCTGAACAACAA GCTGTTCCAGGCGCTTGCCGACGCCATCGGCCGGCCCGCGATGGCGCACGAC CCGCGCTTTGCCGATGACGAAACGCGCTGCCGCTTCGAGTCCGACCTGCGCGC CATGCTGGAAAACTGGTCGGCCACGCGCACGGTGGCGGAGGTGAACGCAGCG CTCGGCGCCGCCGGCATCCCGGTCGCACCGATCCGCAATGTCAAGGAAGCGC TGGAAAGCGAGCACGCGGCGCATCGCCGCCTGCTGACCGAGGTGCCCGACAC GGACGGCGGCACCGTGCGCCTGCCGTCGCAGCCGGTCAAGTTCTCCGCGTAC GGCACCAACCGCGTGACGCCCGCGCCGGCACTGGGCCAGCACACCGGGGCC ATCCTGGCCGCGCCCGATTTCAGTAATTTCAATAAGGAGAAGCAACATGCATAA
[0185] SEQ ID NO: 29 (Succinil Putativo-CoA:oxalato CoA-transferase (SCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.2; Co_CaiB):
ATGGGAGCCTTAAGCCATCTCCGCGTCCTCGACCTGACCCGTGTCCTT GCCGGGCCCTGGTGCGCCCAGAACCTTGCCGACTTCGGCGCCGATGTGATCAA GATCGAGCGCCCCGGCGCCGGCGACGACACCCGCACCTGGGGCCCGCCCTG GCTCAAAGACGAAGACGGCCGCGACACCGCCGAGGCCGCCTACTACCTGGCC GCCAACCGCAACAAGCGCTCGGTCACCTGCGACATCAGCACGCCCGAAGGCCA GCAGATCGTGCGCGACCTCGCCGCGCAGAGCGACGTGGTGCTGGAGAACTAC AAGGTCGGCCAACTCAAGAAATACGGGCTGGACTACGAGTCGCTCAGGCAGGT CAAGCCCGACCTGATCTACTGCTCGGTCACCGGCTTCGGCCAGACCGGCCCGT ACGCCGCGCGCCCCGGCTATGACTTCATCATCCAGGGCATGGGCGGCTTCATG AGCCTGACCGGCGAGCGCGACGACCTGCCCGGCGGCGGCCCGCAGAAGGCC GGCGTGGCGATTTCCGACCTGATGACGGGCCAGTACGCCACCATCGCCGTGCT GGCGGCGCTGGCGCATCGCGAGCGCACCGGCGAAGGCCAGTACATCGACATG GCGCTGCTGGACGTGCAGGTGGCCATGCTGGCCAACATGAACACCAACTACCT GGCCAGCGGCGAGGCGCCGCGCCGCTGGGGCAATGCGCACCCCAACATCGTG CCCTACCAGACCTTCCAGGCCGCCGACGGCTGGATCATCGTCGCGGTGGGCAA CGACGGGCAGTTCCGCAAGTTCGTCACCGACGGCGGCATGCCCGAACTGGCC GACGATCCGCGCTTTGCCAAAAACCCGCAGCGCGTGGCCAACCGCGACGTGCT GGTGCCGATCCTGGCCGAGATGGTGCGCCCGCGCACCCGCGCGCAGTGGATC CGCGACCTGGAAGCCGCGGGCGTGCCGTGCGGCCCGATCAACACGCTCGACG ATGTGTTCGAGGACGACCAGGTCAAGGCGCGCGGCCTGCGCGTGGACCTGCC GCACCCCAGCGCCGGCGAGGTCAAGCTGGTCGCCAGCCCGATCAAGATGAGC GCGACGCCGCCGCAGGCGCTGCGCCACCCGCCGCTGCTGGGCGAGCACACC GACACGGTGCTGGCCGAAACCCTGGGCTACAGCGCCGGGCAGATCGCGGCAC TTCGCGCAAAAGGTGTGCTGTAA
[0186] SEQ ID NO: 30 (Succinil Putativo-CoA:oxalato CoA-transferase (SCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.2; Co_SCACT):
ATGGACAAGGAACGCATTCGCCTGGCGAGGCTGCATGACAAGGTAGTC TCGGCCGACGAGGCCGCCGCGCTGATCCGCGACGGCATGACCGTAGGCATGA GCGGTTTCACCCGCGCGGGCGACTGCAAGGAAGTCCCGTTCGCGCTGGCGCG GCGCGCGGCGACCGAGCCGCTGCGCATCACCCTGATGACGGGCGCCTCGCTC GGCAACGACATCGACCGCGTCCTGGCCGAGGCCGACGTGATCGCGCGCCGCC TGCCGTTCCAGTCCGATGGCACGCTGCGCCGCAAGATCAACGCCGGCGAGGT GATGTTCATCGACCAGCACCTGTCCGAAACCGTCGAGCAGCTGCGTTCCGGCC AGATCGCGCCGGTGGACGTGGCGGTGGTCGAGGCCGTGGCGATTACCGAGCA GGGCGGCATCATCCCCAGCACCTCGGTGGGCAATTCGGCAAGCTTTGCCATGC TGGCGCGCAAGGTGATCGTCGAGATCAACATGAACATGCCGCTGGAGCTGGAA GGGCTGCACGACATCTATTTCCCGGTCCAGCGCCCGTACCGCCAGCCGATCCC GCTGATCGCGCCGGAACAGCGCATCGGACTCCCGTATATTCCGATCGATCCGG AGAAGATTGCCGCCATCGTCATCACCGCCAAGGACGACAGCCCGTCCAATGCG CTGCCGCCGGACGACGAAACCCGCAGCATCGCCGGCCACCTCAATGACTTCCT GCTGCGCGAAGTGCGCGCGGGCAAGCTGTCGCCGTCGCTGCAGCCGCTGCAG GCCGGCATCGGCACCATTGCCAATGCCGTGCTGCACGGGCTGGTCGAGTCGC CGTTCCGCGACCTGAAGATGTATTCCGAGGTGCTGCAGGACAGCACCATCGAG TTGCTCGATGCGGGCCGGCTGTCGTTCGCGTCGGCGTCGTCGGTGACGCTGA CGCGCGAGGTCTACCAGCGCTTCCTGTCGAACCTGGACCGCTACCGTTCGCGC CTGCTGCTGCGCCCGCAGGAGATCAGCAACCATCCCGAGATCCTGCGCCGGCT GGGGCTGATCACCATCAACACCGCGCTGGAGTGCGACATCTACGGCAACGTCA ACTCCACGCATGTGGGTGGCACGCACATGATGAACGGCATCGGCGGCTCCGG CGACTTTGCGCGCAATGCGCACGTGTCGGTGTTCGTGACCAAGTCGGTGGCCA AGGGCGGCGAAGTCTCGAGCATCGTGCCGATGGTGGCGCACGTGGACCACAC CGAGCACGATGTCGACATCATCGTCACCGAGCACGGCCTGGCCGACCTGCGC GGCCTGGCACCGCGCGAGCGCGCGCGCACCGTCATCGCCAATTGCGCCGATC CGCAATACCGCGAGTTGCTGGGCGACTATTTCCGCCGCGCCAGCGCCCACGG CGGCCAGACGCCGCACCTGCTGGAAGAGGCGTTGTCCTGGCATGTCGGCTTG CGCGATCGCGGCACCATGCGGTCCGCACAGCCGGCGCAGGCCCTGGCTGCCT GA
[0187] SEQ ID NO: 31 (Succinil Putativo-CoA:oxalato CoA-transferase (SCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.2; Co_SCMCT):
ATGGTCAACGAAGCGAACAAGGAAAAGATGCCGCTGCAAGGCGTGCGC GTGTTGGAGCTGGGATCGCTGATCGCCGGCCCGTATGCCGGCGGCCTGCTCG CCCAGTTCGGCGCCGAGGTGCTGAAGATCGAAGCGCCGGGCGAAGGCGATCC GCTGCGCAAATGGCGCAAGCTGTACGAGGGCACCTCGCTGTGGTGGTACAGCC AGAGCCGCAACAAGAAGTCGCTGACGCTGGACCTGCGCAGCGCGCAAGGCCA GGACATCGTGCGCAAGCTGGTGGCCGGCGCCGATATCGTGATCGAGAACTTCC GCCCCGGCACGCTGGAGAAATGGGGCCTGGGCTGGGAAGACCTGCGCAAGGT GAATCCGGCCCTGGTCATGGTGCGCATTTCCGGCTATGGCCAGACCGGCCCCT ACAAGGACCGCCCGGGGTTTGCCGCGATCGCGGAATCGATGGGCGGGCTGCG CTATGTCAGCGGCTACCCCGACCGGCCGCCGGTGCGGTCCGGCGTCAGCATC GGCGACACGCTGGCGTCGCTGTACGGCGTGATCGGCGCGCTGCTGGCCATGC ACCATCTGCGCAACAACGATGGCGAAGGGCAGTTTGTGGACGTGGCCCTGTAC GAGTCGGTCTTTGCCGTGATGGAAAGCCTGGTGCCGGAGTTCGACAAGTTCGG CCATGTGCGCGAGCGTTCCGGCGCCAGCCTCCCCGGCATTTCGCCGTCGAACA CATATCCCTGCCAGGATGGACAGTACGTGATCATCGCGGGCAATGGCGACGCC ATCTTCCGGCGCTTCATGACGGCGATCGGCCGCGACGACCTCGGCCAGGATCC GCGCCTGGCTCACAACGATGGCCGCGTGCAGCACAACGCCATGCTCGACGAC GCCATCGCCGCATGGACCGCCGCGCACGACCTCGACACCGTGCTGGCGACGC TGGAAGCCGCGGCGGTGCCTTGCGGGCGCATCTACACCGCGCAGGACATCTG CAGCGACCCCCACTACAGTGCGCGCGGCATGATCGAGCCGCACCAGCTTCCG GACGGCTCGCCGCTGGCCCTGTCGGGCATCGTGCCCAGGCTCAGTGAAACCC CCGGGCGTACCAACTGGATCGGCCCGGCGCTGGGCGAGCACACCGAAGAGAT CCTGGCGGGCATCGGCATTGCCGGCGCCGAATTCCGCGCGCTGCGCGAGGCC GGCGTGGTCTGA
[0188] SEQ ID NO: 32 (Acetil-CoA:oxalato CoA-transferase (ACOCT) de cepa Acetobacter aceti 1023; EC 2.8.3.19; Acesso UniProt A9X6P9; Aa_CoAT3):
MTETTPAKPKGPFDGLLVIDLTHVLNGPFGTTILTDLGARTIKIEPPGHGDDT RTYGPYVGDQSLYFSFVNRGKESIVLNLKDEGDRAIFLEMVRKADVLAENFRPGVM DRLGFNYEELAKINPRLIYASSSGFGQTGPLAHYPAYDTIVQAMSGIMMATGFPDGP PTRVGGTSLSDLCGGVFMFCGIASALYARERTGKGAHIDVSMFDGTLAFLQHALMC WSATGKAPARIGNRHPYMAPFDVFQAQDKPFVICCGNDHLFKALCDVIGAPELATD PRFVENHDRMANNDALKAALEKALSKQPAAHWLDVIHKAGVPVGPLLDVAEAANLP QTAARNMLIKSGGVMMPGNPVKISGYDDPHERPGAPKLDEQGAALRKEFAAPEAK
[0189] SEQ ID NO: 33 (Acetil-CoA:oxalato CoA-transferase (ACOCT) de Escherichia coli MG1655; EC 2.8.3.19; Acesso UniProt P76518; Ec_yfdE):
MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPPGHGDDT RTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFRPGTME KLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYPDAPP VRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMAYIA TGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPRFS SNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQARN MLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS
[0190] SEQ ID NO: 34 (Formiato desidrogenase de Saccharomyces cerevisiae S288C; EC 1.2.1.2; Acesso UniProt Q08911; Sc_FDH1):
MSKGKVLLVLYEGGKHAEEQEKLLGCIENELGIRNFIEEQGYELVTTIDKDP EPTSTVDRELKDAEIVITTPFFPAYISRNRIAEAPNLKLCVTAGVGSDHVDLEAANER KITVTEVTGSNVVSVAEHVMATILVLIRNYNGGHQQAINGEWDIAGVAKNEYDLEDKI ISTVGAGRIGYRVLERLVAFNPKKLLYYDYQELPAEAINRLNEASKLFNGRGDIVQRV EKLEDMVAQSDVVTINCPLHKDSRGLFNKKLISHMKDGAYLVNTARGAICVAEDVAE AVKSGKLAGYGGDVWDKQPAPKDHPWRTMDNKDHVGNAMTVHISGTSLDAQKRY AQGVKNILNSYFSKKFDYRPQDIIVQNGSYATRAYGQKK
[0191] SEQ ID NO: 35 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Bifidobacterium animalis subsp. lactis DSM 10140; EC 2.8.3.16; Acesso UniProt B9X295; Ba_frc):
MADKSTAPLAGIKVIDWTQVQSGPSCTQILAWLGAEVIKLEKVHGGDPTRN EMNDVDGSYSLYFLQLNANKKSITLDMKDPEGKKILTDLLKDADVFVENIGPGDVEK LGFGWDEVHKINPKLIMASLKGFNQGSRFEHVKAFEPVAQCAGGAASTTGWWEGD KNIPTQSGAALGDSNTGMHLTIAILTALLQRERTGEGVFVYQSMQNAVLNLCRIKLR DQLILDHLHQLSYYDCYPGYKFGKAIPRAANAEGGLVLGWCYRAKGWETDPNAYV YIVIQQSQKGFENFCNAMGFQDWLTDPKFSTPNARDEHKQEVYKRVEEYTMQYDK YTLTKELGAKGVPVGPVLDWNELENDPDLNEDGTLITIDQGDARGKFKTIGLPFTMS NYAPDYQRAPKLGENNEEILKSLGYTDEQIADLATKGVIGSNDGVKADLTAAPAQA
[0192] SEQ ID NO: 36 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.16; Co_frc1):
MPAQHTHATPAGPLDGIRVLDLTAVVLGPLATQVLADFGADVIKIEGPEGDL MRANGVSQHAGMSSIYLALNRNKRSVVLDLKIPEGAEALRALIAGADVLVHNMRVA AIERLGFGYEAVARINPRIVYCVATGFGQDGPHRDKPAFDDIIQAGCGLVALGAGNG ERPEYVPSLIADKTTGLALANAVLAALLHRERHGVGQSVEVPMLETMAAFVMTEHL GGLTFEPAPAGAGYARLLQGGRRPAPTRDGWICALPYTERHWHAFFRAVGRDDLA DRYEVGDRAQRNANIRALYGHLAELTPERSTAEWMALFEALDIPATPIYDLDALVDH PHLRAVGLFQATQHPTEGPLREVRPAARFSATPLSLRRHAPALGEHTAEVLQEAGI ALPAAHAGTPAGAQE
[0193] SEQ ID NO: 37 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.16; Co_frc2):
MNLPLNGIKIIDFTHVQAGPACTQLLAWFGADVIKVERPGSGDVTRTQLRDI PDVDALYFTMLNSNKRSLTLDTKKPEGKKILEQLIRESDVLVENFGPGALDRMGFSW ERINELNPKMIVASVKGFSDGHHYEDLKVYENVAQCAGGAASTTGFWDGPPTVSA AALGDSNTGMHLAIGILTALIGRDKTGKGQKVAVSMQDAVLNLCRVKLRDQQRLDR LGFLEEYPQYPHGSFSDVVPRGGNAGGGGQPGWVLKCKGWETDPNAYIYFTIQG HAWEPICKALGKPEWIDDPNYATAKARQPHIFDIFNTIEEWLADKTKYEAVDILRKHD IPCSPVLSMKEIAADPSLRASGSITEVPHKERGTYLTVGSPIKFSDLKPEITGSPLLGE HSEEVLAGLGYGTDDIKRLRESQVI
[0194] SEQ ID NO: 38 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Escherichia coli MG1655; EC 2.8.3.16; Acesso UniProt P69902; Ec_frc):
MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRHQLR DIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGFT WEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSA AALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLD KLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQE QNWENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDI PCAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLG EHTAAVLQELGYSDDEIAAMKQNHAI
[0195] SEQ ID NO: 39 (Formil-CoA:oxalato CoA-transferase (FCOCT) de Oxalobacter formigenes DSM 4420; EC 2.8.3.16; Acesso UniProt O06644; Of_frc):
MTKPLDGINVLDFTHVQAGPACTQMMGFLGANVIKIERRGSGDMTRGWLQ DKPNVDSLYFTMFNCNKRSIELDMKTPEGKELLEQMIKKADVMVENFGPGALDRMG FTWEYIQELNPRVILASVKGYAEGHANEHLKVYENVAQCSGGAAATTGFWDGPPTV SGAALGDSNSGMHLMIGILAALEMRHKTGRGQKVAVAMQDAVLNLVRIKLRDQQRL ERTGILAEYPQAQPNFAFDRDGNPLSFDNITSVPRGGNAGGGGQPGWMLKCKGW ETDADSYVYFTIAANMWPQICDMIDKPEWKDDPAYNTFEGRVDKLMDIFSFIETKFA DKDKFEVTEWAAQYGIPCGPVMSMKELAHDPSLQKVGTVVEVVDEIRGNHLTVGA PFKFSGFQPEITRAPLLGEHTDEVLKELGLDDAKIKELHAKQVV
[0196] SEQ ID NO: 40 (Oxalato decarboxilase (OXDC) de Bacteroides subtilis 168; EC 4.1.1.2; Acesso UniProt O34714; Bs_oxdC):
MKKQNDIPQPIRGDKGATVKIPRNIERDRQNPDMLVPPETDHGTVSNMKFS FSDTHNRLEKGGYAREVTVRELPISENLASVNMRLKPGAIRELHWHKEAEWAYMIY GSARVTIVDEKGRSFIDDVGEGDLWYFPSGLPHSIQALEEGAEFLLVFDDGSFSENS TFQLTDWLAHTPKEVIAANFGVTKEEISNLPGKEKYIFENQLPGSLKDDIVEGPNGEV PYPFTYRLLEQEPIESEGGKVYIADSTNFKVSKTIASALVTVEPGAMRELHWHPNTH EWQYYISGKARMTVFASDGHARTFNYQAGDVGYVPFAMGHYVENIGDEPLVFLEIF KDDHYADVSLNQWLAMLPETFVQAHLDLGKDFTDVLSKEKHPVVKKKCSK
[0197] SEQ ID NO: 41 (Oxalato oxidase (OXO) de Hordeum vulgare; EC
1.2.3.4; Acesso UniProt P45850; Hv_OxOx):
MSDPDPLQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTS TPNGSAVTELDVAEWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGEL LVGILGSLDSGNKLYSRVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIV FVPLTLFGSDPPIPTPVLTKALRVEAGVVELLKSKFAGGS
[0198] SEQ ID NO: 42 (Oxalato oxidorredutase (OOR) da subunidade alfa de Moorella thermoacetica ATCC 39073; EC 1.2.7.10; Acesso UniProt Q2RI41; Mt_OORA!):
MGKVRNISGCVAVAHGVRLADVDVICSYPIRPYTGIMSELARMVADGELDA EFVHGEGEHAQLSVVYGASAAGARVFTGSSGVGVTYAMEVYSPISGERLPVQMAIA DRTLDPPGDFGEEHTDAECCRDQGWIQGWASTPQEALDNTLIYYRVGEDQRVLLP QYACLDGYFVSHILGPVDIPDEAQVKEFLPPYKNHHVLDPRKPQIIGPQIEPAMGPPL QYQRYQAVKGVHKVLEEACDEFARIFGRKYDPYLDEYLTDDAEVIIFGQGAHMETA KAVARRLRNLGEKVGVARLRTFRPFPTEQIKERLSKFKAIGVLDVSANFGISCSGGV LLSELRAALYDYGDKVKTVGFVAGLGGEVVTHDEFYRMFQKLKEIAKTGKVEQTSY WIPFEL
[0199] SEQ ID NO: 43 (Oxalato oxidorredutase (OOR) da subunidade beta de Moorella thermoacetica ATCC 39073; EC 1.2.7.10; Acesso UniProt Q2RI42; Mt_OORB):
MMDRIASIKKAPDEEYYVPGHRTCAGCGPALTYRLVAKAAGPNTIFIGPTGC MYVANTSYGCGPWRVPWIHAQITNGGAVASGIEAAYKAMIRKKKTDAEFPNIIVMAG DGGAVDIGLQALSAMLYRGHDVLFICYDNESYANTGIQTSPTTPYGANTTFTPPGEV VPEGKKLFPKDNPKVIAHGHPELKYVATASIGWPVDLMNKVRKGLNQEGPAYIHIHA PCPKGWQFPADKTIEMAKLAVQTGMFQLYEYENGEYKLSVKVDKRKPVSEYMKLQ KRFAHLKPEHIAKMQAFVDARCAEVGITVPVVASNA
[0200] SEQ ID NO: 44 (Oxalato oxidorredutase (OOR) da subunidade delta de Moorella thermoacetica ATCC 39073; EC 1.2.7.10; Acesso UniProt Q2RI40; Mt_OORD):
MSTKDLFAEPNLKQITVWARGVVMNKDARDIVVALTEAAAKEGKYVQAWE NYVDLPDRIYVPVRAYARISSDPIESKYIYENETPDIVVLVEESLIKGVPILKGIRPGSTL VVNTKRSIDTILEFLGDTGNLAQIVTVDANSMAEAVMTLSGAEGATDATGIGAGIAAPI AGAVVKATGIVDVENLAAVVKNPAAMRRGYAEAQVRQLPPHEAVEEAAVSATELLR QMPFAGTVPSPVTENEGMVTGNWRIQRPIIDREACTECYTCWIYCPDSCITRTEEG PVFNMKYCKGCGLCTAVCPSGALTNVPELDFKD
[0201] SEQ ID NO: 45 (Oxalato--CoA ligase / Oxalil-CoA sintetase (OXS) de Arabidopsis thaliana col-1; EC 6.2.1.8; Acesso UniProt Q9SMT7; At_AAE3):
MDSDTLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDA GIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTS KEGNAPAQEAASKLKISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALF LHTSGTTSRPKGVPLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSL GAGAAVTLPAAGRFSATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLR FIRSCSASLAPVILSRLEEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKP VGQEMAILNEKGEIQEPNNKGEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIG YFDTDGYLHLVGRIKELINRGGEKISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEIN CAVIPREGTTVTEEDIKAFCKKNLAAFKVPKRVFITDNLPKTASGKIQRRIVAQHFLEK P
[0202] SEQ ID NO: 46 (Oxalato--CoA ligase / Oxalil-CoA sintetase (OXS) de Methylobacterium extorquens AM1 / DSM 1338; EC 6.2.1.8; Acesso UniProt C5APP0; Me_oxs):
MTMLLPEPSALLPTDSKEASVPATTLHELIQAGADAAPALSSPGGVPLTFQA LRALTERTVADLNARGIGRGDRVAIVLPNGPEMAAAFIAVAAGTTSAPLNPSYKADE FEFYMSDLGAKLLLVAEGSETPAVAVAEKLGVSVARLRPTPDEGAGSFTLHFASEST GPTEKSGPAGSDDIALVLHTSGTTSRPKIVPLTQANVCASARNIRTALAFGPEDRGL NIMPLFHIHGLIAGILAPLSVGGQVSCTPGFNALKFFGWMDEVNPTWYTGVPTMHQ AILGRAARNKEIIARNPLRFIRSSSSSLPPQVMKELEETFGAPVIEAYGMTEAAHQMA SNPLPPKPHYAGSVGLAAGPEIAVVDLDGEPLPAGETGEIVIRGDNVMKGYENNEK ANAEAFTKQGWFRTGDQGVLSPEGYLSITGRLKEIINRGGEKISPREVDEILMDHPA VSQCVTFAVPHDKLGEDVAAAIVLREGVEAVEKDIRSFASERLAAFKVPAKILILDEIP KGATGKLQRIGLAQKLGLV
[0203] SEQ ID NO: 47 (Oxalato--CoA ligase / Oxalil-CoA sintetase (OXS) de Saccharomyces cerevisiae S288C; EC 6.2.1.8; Acesso UniProt P38137; Sc_YBR222C):
MTSAATVTASFNDTFSVSDNVAVIVPETDTQVTYRDLSHMVGHFQTMFTNP NSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAPLNPNYKEKEFNFYLNDL KSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVEYDIYSPEDNYKRVIYR SLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYK LTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDGFHPKLFWDQFVKYNCNWF SCVPTISMIMLNMPKPNPFPHIRFIRSCSSALAPATFHKLEKEFNAPVLEAYAMTEAS HQMTSNNLPPGKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVSIRGENVTLGY ANNPKANKENFTKRENYFRTGDQGYFDPEGFLVLTGRIKELINRGGEKISPIELDGIM LSHPKIDEAVAFGVPDDMYGQVVQAAIVLKKGEKMTYEELVNFLKKHLASFKIPTKV YFVDKLPKTATGKIQRRVIAETFAKSSRNKSKL
[0204] SEQ ID NO: 48 (Oxalato:formiato antiportadora (oxlT) de Bifidobacterium animalis subsp. lactis DSM 10140; Acesso UniProt B9X293; Ba_OxlT):
MTKILIDDIIPIIVIMALGYVCGKLSYFDNDQRQGLNKLVLNIALPAALFISIVKA TREMLAQDAVLTILGFIGIIVMFMLSYYLCRLMFHHSIQEAAVCALIAGSPTIGFLGFAV LDPIYGDTVSTNLVIAIISIVVNAVTIPIGMYLINLGQSKDRERLSKAAVTNSKGQVSIAN PKDDIAVDPNKDAKTDKTAEVMISKSSNMGKKKNQNLEALINALKQPVCWAPLLAIV LVLIGVRVPSGFAPTFDLIAKANSGVAVLAAGLALSTVKFSLGWETIWNTFYRLILTPA AFLGVGLLLGMGSNVNKLSMLVMAVALPPAFSGIIISSRYNIYVKEGASTTAVSTVAF AVTCLLWIWLVPLCCH
[0205] SEQ ID NO: 49 (Oxalato:formiato antiportadora (oxlT) de Cupriavidus oxalaticus ATCC 11883; Co_OxlT1):
MSNATAGALSPNQGASESTRWIQLLVGVVCMIATANIQYAWTLFVPEIQDT YGWSRASIQIAFTVFVLVQTWLAPIEGYFIDKFGPRMMVAFGALFIGTAWVINSQATT LMGFYVGAAVGGLGVGSIYATCINNALKWFPDRRGLAVGLTAGGYGAGSAATILPIA AMIESQGFQHTFLFFGLLQGSLAFVAAWFLRSPKTGEVRGSKKLAQATRDYTLKEA LCTRLFWLMLVMFVLVVTGGMMAVAQLGVIAKDLGVKEFQVDLHFFVMAALPLALM LDRIMNGISRPLFGWISDNIGREKTMVIAFTLEGLGIIALGYFGSNPYAFLILSGVVFLA WGEVYSLFSALAGDAFGTKHIGKIYGVLYTAKGIGALFVPIGNLLMEATGTWSTVLYT VAVMDLTAAFLAIMVLRPVLASHVATSRQRFAQESAAAGAQVAA
[0206] SEQ ID NO: 50 (Oxalato:formiato antiportadora (oxlT) de Cupriavidus oxalaticus ATCC 11883; Co_OxlT2):
MELQQRESTSRFASPWVQLVFGVICMAMIANMQYGWTLFVNPIDDKYHW GRTAIQVAFTIFVVTETWLVPIEGYLVDKYGPRPVVVGGGLLCAIAWALNSVASSLP MLYFAAAIGGVGAGAVYGTCVGNALKWFPNRRGLAAGITAAGFGAGSAMTVVPIAN MIKTSGYEATFLWFGLGQGLIVFILGLALYPPSARILSEVKTTLKAAATYNATPRQVLK SPIFWIMYAMFVMMAAGGLMATAQLGPIAKDFGLHDSPVSILGLTLPALTFALTIDRV LNGLTRPFFGWISDHIGRERTMFFAFAVEAVGILLLSKYGHNPVAFVVLTGIVFFAWG EIYSLFPATCGDTFGPKFAATNAGLLYTAKGTAALLVPFSSVITAATGDWHAVFMLA SGMAALSAVLALFVLKPMREAHARKYVHANTAAPMGYRAVQEDLT
[0207] SEQ ID NO: 51 (Oxalato:formiato antiportadora (oxlT) de Escherichia coli MG1655; Acesso UniProt P37662; Ec_YhjX):
MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAPVSQVAFSF GLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMMLWLSAGVL VGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLETVGLEKT FVIWGAIALLMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWMLAVMF LTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVIT IGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYG VIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQKMLREAHGSL
[0208] SEQ ID NO: 52 (Oxalato:formiato antiportadora (oxlT) de Oxalobacter formigenes DSM 4420; Acesso UniProt Q51330; Of_oxlT):
MNNPQTGQSTGLLGNRWFYLVLAVLLMCMISGVQYSWTLYANPVKDNLGV SLAAVQTAFTLSQVIQAGSQPGGGYFVDKFGPRIPLMFGGAMVLAGWTFMGMVDS VPALYALYTLAGAGVGIVYGIAMNTANRWFPDKRGLASGFTAAGYGLGVLPFLPLIS SVLKVEGVGAAFMYTGLIMGILIILIAFVIRFPGQQGAKKQIVVTDKDFNSGEMLRTPQ FWVLWTAFFSVNFGGLLLVANSVPYGRSLGLAAGVLTIGVSIQNLFNGGCRPFWGF VSDKIGRYKTMSVVFGINAVVLALFPTIAALGDVAFIAMLAIAFFTWGGSYALFPSTNS DIFGTAYSARNYGFFWAAKATASIFGGGLGAAIATNFGWNTAFLITAITSFIAFALATF VIPRMGRPVKKMVKLSPEEKAVH
[0209] SEQ ID NO: 53 (Oxalil-CoA decarboxilase (OXC) de Bifidobacterium animalis subsp. lactis DSM 10140; EC 4.1.1.8; Acesso UniProt B9X297; Ba_oxc):
MVDVSVTATSSDQNLTDSPHYLAETLIKNGVKHMYGVVGIPVTDFARIAQG MGIRFIGMRHEEDAVNAAAAEGFLTGRPAVALTVSAPGFLNGLAPLLEATTNGFPVI MIGGSSTRHVVDMHEGEYEGLDQMNYAKQFCKESFRIDKIEDIPLAVARAMHIACS GRPGGVYIDFPDDAVAQTLDKDVAESQLWVANQPAPAMPPAQSSVDEALKLLSEA KNPLMLVGKGAALAQAEDELREFVEKTDMPFQPMSMAKGVIPDDDPHCTASCRGL ALRTADVVLLVGARLNWMLNFGEGKEWNPNVKFIQIDIDPNEIENARSIACPVVGDIK SAMQMINAGLEKTPVKASAQWLDMLKADAEKNDAKFAARVNSNTVPMGHYDALGA IKKVYDQHKDMILTNEGANTLDDCRNIIDIYQPRHRLDCGTWGVMGCAVGYSIGAAV ATGKPVLYVGGDSGFGFDGMEVEVACRYNLPITFVVLNNGGIYRGDFENLGDDGD PSPLTLSYDAHYERMIEAFGGNGYYATTPAEVEQMVGEAVASGKPSLVHVQLADYA GKESGHISNLNPKPVVGPLATSEMTANPYLKGAHM
[0210] SEQ ID NO: 54 (Oxalil-CoA decarboxilase (OXC) de Cupriavidus oxalaticus ATCC 11883; EC 4.1.1.8; Co_oxc):
MAEVGNELQRHAAEEHQAQTDGFHLVIDALKLNGIENIYGLPGIPVTDLARL AQANGMRVISFRHEQNAGNAAAIAGFLTQKPGVCLTVSAPGFLNGLTALAHATTNC FPMILISGSSEREIVDLQQGDYEEMDQLAIARPHAKAAFRVLHAEDIGVGIARAIRAAV SGRPGGVYLDLPAKLLGQSMEAEKGRKSLIKVVDPAPRQLPAPDSVDRAVALLKSA KRPLILVGKGAAYARAEADIRTLVEKTGIPYLPMSMAKGLLPDTHPQSASAARSYVL AEADVVLLVGARLNWLLSHGKGKTWGKPKQFIQIDIAPTEMDSNVAIAAPVVGDIGS CVSAILDKVGDDFARPGADWLNAVADRRDTNLAKMAETLARSRDASPMNFHGALG VLKDVVKANPNISFVNEGANTLDYARAVIDMYEPRKRLDVGTWGVMGVGMGYAVA AAVETGKPVLALCGDSAFGFSGMEVETICRYNLPVCIVIFNNNGVYKGIDVNPTGGR DPAVTTFVPGARYDKMMEAFGGVGANVTTPAELEAAVNEALRSGKPTLVNAVIDPA AGTESGRLTNLNPQSSAKK
[0211] SEQ ID NO: 55 (Oxalil-CoA decarboxilase (OXC) de Escherichia coli MG1655; EC 4.1.1.8; Acesso UniProt P0AFI0; Ec_oxc):
MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFR HEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRA IVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLD LPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIILGKGAAY SQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVMLVGARL NWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTF TTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLVNEG ANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAFG FSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDAF RGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN
[0212] SEQ ID NO: 56 (Oxalil-CoA decarboxilase (OXC) de Oxalobacter formigenes DSM 4420; EC 4.1.1.8; Acesso UniProt P40149; Of_oxc):
MSNDDNVELTDGFHVLIDALKMNDIDTMYGVVGIPITNLARMWQDDGQRFY SFRHEQHAGYAASIAGYIEGKPGVCLTVSAPGFLNGVTSLAHATTNCFPMILLSGSS EREIVDLQQGDYEEMDQMNVARPHCKASFRINSIKDIPIGIARAVRTAVSGRPGGVY VDLPAKLFGQTISVEEANKLLFKPIDPAPAQIPAEDAIARAADLIKNAKRPVIMLGKGA AYAQCDDEIRALVEETGIPFLPMGMAKGLLPDNHPQSAAATRAFALAQCDVCVLIGA RLNWLMQHGKGKTWGDELKKYVQIDIQANEMDSNQPIAAPVVGDIKSAVSLLRKAL KGAPKADAEWTGALKAKVDGNKAKLAGKMTAETPSGMMNYSNSLGVVRDFMLAN PDISLVNEGANALDNTRMIVDMLKPRKRLDSGTWGVMGIGMGYCVAAAAVTGKPVI AVEGDSAFGFSGMELETICRYNLPVTVIIMNNGGIYKGNEADPQPGVISCTRLTRGR YDMMMEAFGGKGYVANTPAELKAALEEAVASGKPCLINAMIDPDAGVESGRIKSLN VVSKVGKK
[0213] SEQ ID NO: 57 (Oxalil-CoA decarboxilase (OXC) de Saccharomyces cerevisiae S288C; EC 4.1.1.8; Acesso UniProt P39994; Sc_YEL020C):
MTTTATQHFAQLLQKYGIDTVFGIVGIPIVQLADTMVANGIKFIPCRNEQAAS YAASAYGYISDKPGVLLIVGGPGLIHALAGIYNSMSNRWPLLVIAGSSSQSDIHKGGF QELDQVSLLSPFLKFTGKLTPDNIDMITQKALNYCIQGTAGVSYIDVPADFIEYEKPLE GNDRTGNELPMILTPNICGPDPSKIKKVVQLILQHKNKNILIVIGKGAVKNSHEIRRLV NTFNLPFLPTPMAKGIVPDSSPLNVSSARSQALKIADIVLVLGARLNWILHFGTSPKW NSESIFIQFDSNPETLGDNNVSPGADLSIWGDIGLSVTALVEELTRQDSCWKYSGVK QEIREKIQLNQTRLLRKEKTRGAQLNYNQVYGTLRPLIDDYRTILVTEGANTMDIARIS FPTDAPRRRLDAGTNATMGIGLGYALACKASHPELDVVLIQGDSAFGFSAMEIETAV RCQLALVIVVMNNSGIYHGEKDIEGDLPPTALSKNCRYDLVGKGLGANDFFVNTISE LSRCFQQAVQLSRTKRETSVINVIIEPGEQKQIAFAWQNKPRL
[0214] SEQ ID NO: 58 (Oxalil-CoA redutase / Glioxilato:NADP+ oxidorredutase (OXR) de Methylobacterium extorquens AM1 / DSM 1338; EC
1.2.1.17; Acesso UniProt C5APN9; Me_PanE):
MSIAIVGAGAIGGYLGVRLAEAGEDVTFIARSNAAAIQADGMRLIEEDGTEIH SKSVKATRSMQEAGVHEVVLLTVKAHQVGPIAADLHHLIGPDTVVVTMQNGIPWWY FLGGYSGDHAGTRLESADPGGLIADHLDPKHVIGSVVYPATVLTDPGTVKVIEGNRF GLGELDGSKSERVLALSQRLARAGFRAPVTSDIRAEIWLKLWGNLSFNPISALTHAT LEDICRFPDTRAIAAEMMREAEVIANKLGVTFRLGIDKRIAGAEKVGPHKTSMLQDVE AGRPIELEALVGSVIELGRLTGTPTPHIDTVFALMRLLAQSLERAQGRLAIQGA
[0215] SEQ ID NO: 59 (Succinil Putativo-CoA:oxalato CoA-transferase (SCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.2; Co_ACOCT):
MTQSNLPDLPDLPLTGLRVIDFSRVLAGPYCTALLGDLGAEVIKVEPPGGDD YRAVGPFAGGKSGLFCAMNRNKQSIVIDLKTEDGLAVARALCRGADVVVENFRPGV ADKLGIGYAALRELNPSLVYASVSGFGQTGPESHRPAYDIILQAMCGLMDATGAPD GAPTMLGEAVSDAVSGLFASWGVLAALLAREKTGRGTHVDVSMFDATLSLSATLVA RYAATGLAPRRVGNRHPSSAPFGAYRAADGFYVVAVLNNKLFQALADAIGRPAMA HDPRFADDETRCRFESDLRAMLENWSATRTVAEVNAALGAAGIPVAPIRNVKEALE SEHAAHRRLLTEVPDTDGGTVRLPSQPVKFSAYGTNRVTPAPALGQHTGAILAAPD FSNFNKEKQHA
[0216] SEQ ID NO: 60 (Succinil Putativo-CoA:oxalato CoA-transferase (SCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.2; Co_CaiB):
MGALSHLRVLDLTRVLAGPWCAQNLADFGADVIKIERPGAGDDTRTWGPP WLKDEDGRDTAEAAYYLAANRNKRSVTCDISTPEGQQIVRDLAAQSDVVLENYKVG QLKKYGLDYESLRQVKPDLIYCSVTGFGQTGPYAARPGYDFIIQGMGGFMSLTGER DDLPGGGPQKAGVAISDLMTGQYATIAVLAALAHRERTGEGQYIDMALLDVQVAML ANMNTNYLASGEAPRRWGNAHPNIVPYQTFQAADGWIIVAVGNDGQFRKFVTDGG MPELADDPRFAKNPQRVANRDVLVPILAEMVRPRTRAQWIRDLEAAGVPCGPINTL DDVFEDDQVKARGLRVDLPHPSAGEVKLVASPIKMSATPPQALRHPPLLGEHTDTV LAETLGYSAGQIAALRAKGVL
[0217] SEQ ID NO: 61 (Succinil Putativo-CoA:oxalato CoA-transferase (SCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.2; Co_SCACT):
MDKERIRLARLHDKVVSADEAAALIRDGMTVGMSGFTRAGDCKEVPFALAR RAATEPLRITLMTGASLGNDIDRVLAEADVIARRLPFQSDGTLRRKINAGEVMFIDQH LSETVEQLRSGQIAPVDVAVVEAVAITEQGGIIPSTSVGNSASFAMLARKVIVEINMN MPLELEGLHDIYFPVQRPYRQPIPLIAPEQRIGLPYIPIDPEKIAAIVITAKDDSPSNALP PDDETRSIAGHLNDFLLREVRAGKLSPSLQPLQAGIGTIANAVLHGLVESPFRDLKM YSEVLQDSTIELLDAGRLSFASASSVTLTREVYQRFLSNLDRYRSRLLLRPQEISNHP EILRRLGLITINTALECDIYGNVNSTHVGGTHMMNGIGGSGDFARNAHVSVFVTKSV AKGGEVSSIVPMVAHVDHTEHDVDIIVTEHGLADLRGLAPRERARTVIANCADPQYR ELLGDYFRRASAHGGQTPHLLEEALSWHVGLRDRGTMRSAQPAQALAA
[0218] SEQ ID NO: 62 (Succinil Putativo-CoA:oxalato CoA-transferase (SCOCT) de Cupriavidus oxalaticus ATCC 11883; EC 2.8.3.2; Co_SCMCT):
MVNEANKEKMPLQGVRVLELGSLIAGPYAGGLLAQFGAEVLKIEAPGEGDP LRKWRKLYEGTSLWWYSQSRNKKSLTLDLRSAQGQDIVRKLVAGADIVIENFRPGT LEKWGLGWEDLRKVNPALVMVRISGYGQTGPYKDRPGFAAIAESMGGLRYVSGYP DRPPVRSGVSIGDTLASLYGVIGALLAMHHLRNNDGEGQFVDVALYESVFAVMESL VPEFDKFGHVRERSGASLPGISPSNTYPCQDGQYVIIAGNGDAIFRRFMTAIGRDDL GQDPRLAHNDGRVQHNAMLDDAIAAWTAAHDLDTVLATLEAAAVPCGRIYTAQDIC SDPHYSARGMIEPHQLPDGSPLALSGIVPRLSETPGRTNWIGPALGEHTEEILAGIGI AGAEFRALREAGVV
[0219] SEQ ID NO: 63 (pZR748 - vetor de expressão Sc_YBR222C, Sc_YEL020C, Of_oxlT):
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGAC TGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTA ATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTG CAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATT ATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAAT GACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGAT AATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTA TCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGT ACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGG AATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCT TGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCT AAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTA AAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACC AGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTT ATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCC CTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCA CCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTT GAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATG CCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCA GGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTT GTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGC TGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTC TTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACC TGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAA TCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCA CCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCG AAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAG CTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAG GTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTAT CAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATC GCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGG CCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGA AGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAG GTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGA GTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGT TCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAG CCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGG GCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATC TATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAG TGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATG AAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGAC CACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACA GTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCC AACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCC TCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGC CCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGG CCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGT TTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAA GTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATA CAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTAT GAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTA ACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTT ATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAA AAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCC ACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCT GCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGA TTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATT CATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGG TGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGA GGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAAT ACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAG AGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGA TGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCG TATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGC GACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGA ACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGA AATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAA TAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGC ACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGA TTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCA GCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGG CGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTC CTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCT GGCGTGGTGCTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGG CCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCT ATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGT GTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGA AAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATA ATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGG TATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACT GTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGAC GGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTA CTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTT CTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCT CTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAAC ACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGC TGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCT GAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTAT CCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAA AAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACA CCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGC TGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGT GTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCT TTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTT TCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCG ATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCT ACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCA GAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTG GTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTA CTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAAT GGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACA TTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACT GCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTG GTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCA GCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAG TGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGC TGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCT GTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCG AAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACA AAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAG GTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAAC AAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTT TCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATAC CTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTC AGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATA TACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAA CATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAG TAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAA TGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAA AGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAA ATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTA TCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATA AAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTT TTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAAT GTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATT TCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAG CACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAA AAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTA CAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAAT ATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTA AAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGG TGGAGGGGAATTACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTA TAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGT AGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTG CCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGG TACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTA GTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAG GTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAA ATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATC GTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACA TCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAG AACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGG TAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGG TGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTT CTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGT GCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCA AACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCC TCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCA CTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGG CTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGG GTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGG CATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTT GGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATC CGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACG GATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGC AAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCC AAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTAC TCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGT GAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAG ATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTG TTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAA TCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTT TACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGA TTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTAT CAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAG GCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCC AACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAG AAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATT TCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCG CATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGC GATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGA ACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATAC CTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGG AGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTT TAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTC AGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCT GATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGT TGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAA CACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATA TTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTT GAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTT GATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTA CTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCA TGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAA TAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAG AAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACA ACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTT TGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
[0220] SEQ ID NO: 64 (pZR753 - vetor de expressão Ec_frc, Of_oxc, Sc_YBR222C, Of_oxlT):
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGAC TGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTA ATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTG CAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATT ATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAAT GTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGC CCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAAC GTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATC GATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATA CCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGAT ATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGG GAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTG ATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAG CGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGC GCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTT GCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTC AATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCG TCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTT GGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCT GGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCAC TATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATG GATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGA TATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTG GCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAA TTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAAC CGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTAC GCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCA GGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCAT CTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGAC TGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAA AGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATG GTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATA TTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCC GGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCC ATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTG GCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAA GCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTT CTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAA GGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTAC CCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATT GATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATT GCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAAT AAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGT GACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTT CAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGA TGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATC ACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGT CACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAA CCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCC CTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGT GAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATG AATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACA TTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGT GGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAA GAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCT GCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCC AGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCC GCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAA GGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGC ACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGAT GCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGA TCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTG TTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTC CAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAAC AAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAA CTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATAT TTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGA CATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTAT TGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCG CTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCT GCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAA AGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCG TGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAA TACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACC ATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTAT CAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGAT TAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTG CTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTT TACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAAT TTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAA ACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGA AAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTA CTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTAT TGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGG TCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTC AGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCT TTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTAT AAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCG TCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCAC GTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGA GTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTG AACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTT CCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAA CGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACAC TTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTC CATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGG TTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTG TAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAA CCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTC CAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCT ATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAA AGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTA GATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGA GGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAAC TTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCT GAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATC GATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAA GCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAAC TTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTG ATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAA CTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTA GAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAAT CCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTA CAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTT AAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAAC AAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAA AGAGATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATAATCCACAAACAG GACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTT GCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCC GGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACT CTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAA ATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTG GACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTG GCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAG ATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACG GTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAG GTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCC TGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCG TTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTG GGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGC CAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGA TCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTT TCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAA TGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTT TATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTT CCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGG TTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGC TGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACT TCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAG TCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTA GCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTATGACTAAA CGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCC ATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGC AGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAAC ACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGG ATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCG AAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTAT GCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTC TCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTAT CGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCA TTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTG CATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGAC CGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGA GAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGA TACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATT CGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAA AGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCA TAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTT TACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCT AAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGT AAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTG GCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGT TCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGA TACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTA GCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAA TTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGA AACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAAT CAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTT CGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAAT AGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGG GAATTACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGAT TAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTT CATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGAT TCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAG CAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTT TCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCG TTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATG TCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGC TGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTC TTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGA AAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTT CAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTC AGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGC TCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTC TCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTT GAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCT GCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTA CAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTT CACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGG CATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTAT TTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATC CGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGC CCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTA CGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGAT GTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACA CCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTG TAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATA AGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAA CAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAA GATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAG AAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTC CTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAA CTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCAT ATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCA TAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAAT ACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCA TGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAG ACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACC AAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCC AGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATG CTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGG ATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTG ACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACA ACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCC CGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATT TAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCT TGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATC TTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAAT CGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTAC GTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTC TCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAAC GTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAA TATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAG AATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAG GGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACA CAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
[0221] SEQ ID NO: 65 (pZR761 - vetor de expressão Sc_YBR222C, Sc_YEL020C):
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGAC TGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTA ATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTG CAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATT ATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAAT GACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGAT AATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTA TCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGT ACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGG AATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCT TGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCT AAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTA AAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACC AGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTT ATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCC CTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCA CCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTT GAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATG CCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCA GGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTT GTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGC TGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTC TTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACC TGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAA TCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCA CCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCG AAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAG CTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAG GTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTAT CAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATC GCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGG CCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGA AGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAG GTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGA GTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGT TCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAG CCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGG GCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATC TATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAG TGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATG AAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGAC CACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACA GTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCC AACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCC TCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGC CCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGG CCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGT TTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAA GTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATA CAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTAT GAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTA ACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTT ATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAA AAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCC ACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCT GCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGA TTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATT CATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGG TGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGA GGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAAT ACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAG AGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGA TGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCG TATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGC GACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGA ACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGA AATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAA TAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGC ACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGA TTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCA GCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGG CGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTC CTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCT GGCGTGGTGCTCGCATAGTAGACTAGAGATCTATGTGAGGATCCTAACTCGAGA TCGAGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGG TTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAG CCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAA GGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCT GCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGC AAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAAT GACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGTCCATTTAT GTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGCAAAACCA TATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATACTCATAAT AAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAAAGAAGA TATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAACTTCCTA AAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACCTACGTT TCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTGTTAGAT ATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACATATTAAT ACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAAGGTGC GTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATATTATA AATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGAGAATC TGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATGGTGAC ACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTCGGGCT TCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACACTCCGG GACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGATGGAG CAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTTGCTGT TCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGATTGAC CGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATCTGTCT CAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGTTTGCT CCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGACGACAA TATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATTGGAAT ACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATGGATCA GCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTTTATAC TTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAGAGCA TGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCTGAGA CTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAAAATC TAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCCAATG ATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAGGTGC TGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATCCTCC GTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGAATAC ATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGATGAAT TGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGCTGGA AGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAAAGAA CGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAACCCT TATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGGGTTG CAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAATGCCG TTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAAGTGA CTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTACACC AGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAACAGTC AGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCCAAGC GGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGAAGAT ATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAATACAA GTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTTTTAT GGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATATCCG CATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAAAAT AACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATT AAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTC ATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATT CCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGC AAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTT CCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGT TCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGT CCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCT GTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCT TTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAA AACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTC AGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCA GTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCT CCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCT CATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTG AAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTG CAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTAC AAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTC ACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGC ATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATT TCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCC GTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCC CGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTAC GTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATG TTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACAC CCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGT AATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAA GTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAAC AAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAG ATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGA AAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCC TGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACT CATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATAT TTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATA GGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATAC AACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATG AGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGAC TTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAA CCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTA AAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAG CGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCT GTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATA AAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACC ATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACT CTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGA CATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAA TCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGT ATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTG TGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGA ACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTA CTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCA TGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTA CTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATAT AAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAAT ATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGA TGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAG GAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
[0222] SEQ ID NO: 66 (pZR856 - vetor de expressão Of_oxlT):
GATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATT GGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTGAAATTTAAAG TTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAG TAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAG ATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATAATCCACAAACAGGACA ATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTG ATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTT AAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCT CAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTC GGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACC TTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCG GTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGT TCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTG GGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTT GGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATC GCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTT ACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTT CTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACA GCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGT GTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTT CCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGT TGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATC GACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTC TGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAAT TGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTC ATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAG AAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGA TTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTATGACTTGAGACC GGCTATTACGAGCGCTTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGG TTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAG CCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAA GGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCT GCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGC AAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAAT GACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGTCCATTTAT GTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGCAAAACCA TATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATACTCATAAT AAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAAAGAAGA TATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAACTTCCTA AAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACCTACGTT TCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTGTTAGAT ATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACATATTAAT ACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAAGGTGC GTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATATTATA AATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGAGAATC TGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATGGTGAC ACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTCGGGCT TCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACACTCCGG GACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGATGGAG CAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTTGCTGT TCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGATTGAC CGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATCTGTCT CAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGTTTGCT CCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGACGACAA TATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATTGGAAT ACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATGGATCA GCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTTTATAC TTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAGAGCA TGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCTGAGA CTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAAAATC TAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCCAATG ATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAGGTGC TGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATCCTCC GTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGAATAC ATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGATGAAT TGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGCTGGA AGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAAAGAA CGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAACCCT TATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGGGTTG CAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAATGCCG TTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAAGTGA CTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTACACC AGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAACAGTC AGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCCAAGC GGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGAAGAT ATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAATACAA GTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTTTTAT GGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATATCCG CATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAAAAT AACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATT AAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTC ATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATT CCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGC AAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTT CCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGT TCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGT CCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCT GTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCT TTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAA AACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTC AGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCA GTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCT CCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCT CATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTG AAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTG CAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTAC AAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTC ACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGC ATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATT TCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCC GTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCC CGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTAC GTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATG TTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACAC CCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGT AATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAA GTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAAC AAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAG ATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGA AAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCC TGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACT CATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATAT TTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATA GGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATAC AACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATG AGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGAC TTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAA CCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTA AAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAG CGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCT GTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATA AAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACC ATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACT CTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGA CATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAA TCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGT ATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTG TGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGA ACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTA CTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCA TGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTA CTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATAT AAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAAT ATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGA TGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAG GAACACTTAACGGCTGACATGGGGCGGCCGCTCAACGTACCGGTCTCAGTAGG GAGAGCTGTATGTGGGTAG
[0223] SEQ ID NO: 67 (pZR878 - vetor de expressão Ec_frc, Of_oxc, Sc_YBR222C, Sc_YEL020C, At_AAE3, Ec_yfdE):
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGAC TGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTA ATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTG CAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATT ATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAAT GTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGC CCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAAC GTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATC GATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATA CCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGAT ATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGG GAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTG ATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAG CGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGC GCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTT GCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTC AATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCG TCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTT GGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCT GGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCAC TATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATG GATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGA TATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTG GCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAA TTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAAC CGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTAC GCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCA GGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCAT CTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGAC TGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAA AGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATG GTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATA TTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCC GGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCC ATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTG GCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAA GCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTT CTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAA GGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTAC CCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATT GATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATT GCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAAT AAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGT GACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTT CAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGA TGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATC ACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGT CACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAA CCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCC CTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGT GAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATG AATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACA TTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGT GGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAA GAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCT GCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCC AGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCC GCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAA GGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGC ACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGAT GCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGA TCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTG TTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTC CAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAAC AAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAA CTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATAT TTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGA CATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTAT TGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCG CTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCT GCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAA AGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCG TGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAA TACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACC ATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTAT CAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGAT TAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTG CTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTT TACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAAT TTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAA ACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGA AAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTA CTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTAT TGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGG TCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTC AGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCT TTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTAT AAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCG TCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCAC GTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGA GTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTG AACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTT CCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAA CGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACAC TTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTC CATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGG TTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTG TAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAA CCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTC CAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCT ATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAA AGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTA GATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGA GGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAAC TTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCT GAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATC GATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAA GCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAAC TTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTG ATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAA CTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTA GAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAAT CCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTA CAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTT AAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAAC AAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAA AGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTAC CCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATT GTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAA TTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGT TATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATC CACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTA ATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTG GATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTG ACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGC AGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTA GAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATAT GTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAA AAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAA ATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTA AGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAG CATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATT GCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGAT TCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCC ATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGC CAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATT CAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTG AATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAA TACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTAC AGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTG GGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGC TGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAG TAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACC ATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACT GCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACA CAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTA AAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAA TTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAA GGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTCTGGGTCTGACGGCCATCA AGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAA TTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAA GTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAA GTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGA GATTCAAAGGTAATATTAACAATAATTTATTTTCAATGGATAGCGATACTCTCTCA GGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTT TCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCC GCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTC ACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTA GAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTT TACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCAC CGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGC TTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTG ACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACA CTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAG CTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTAC GGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGC TCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCA ACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGC CGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAAT ATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATT GTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGA CAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACA AGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAG AAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCA AATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAG TTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGC ATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTC CAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTG CATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTC CAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGA ATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCA AAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGA AGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTT TGACTGGGTGTTCCGATACACTCAATTTGTGTAGGATAAAACGAAAGGCTCAGT CGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGC AATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCT TATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAA ATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTA TTTTCAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCG ATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGG CGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATT TGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAA GAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATAT GCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAA ACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGC TTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGA TACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGC TCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTT ATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGG CGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTC TGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATC CCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTG TTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGA ACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCT ATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGT TAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTG AGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGG GAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCAT GTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGA GTTCTCATCATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTT TTTTTTGACTTAGTTTCAATTTTACATTCCAGGAAGTAGACTAGAGATCTATGTGA GGATCCTAACTCGAGATCGAGACTGCTATGACTAAACGGCGCGCCTGATAGGT GGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCT GTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCA CCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGG GCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTC TACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATAC CGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGG ATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATAT CGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGC TCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTA TATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCT TAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAA GAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTA GTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGA ACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAA ATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATT ACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGA TAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAG AGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTC GTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAA TTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACAT AAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATT TAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCA TTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTA TTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCA GCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGG TTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAA CATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATT TAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAAT GTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTACGAGTCATTGGTAAC TATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTG CCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTG TCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGC AGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGA CTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGAT TTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATG ACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATG GCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTG GTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCG AATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCA GGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCA AGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCG TATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGA GGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCA GGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATC CTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGAT GCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCC AAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCAT GTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAA TTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTT CGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATC TGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTG GCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACAC CGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATT GGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAA GACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTC GTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCG CAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAA GAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATA AAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCC ATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTG TTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACT GCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGT AATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTAT CGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGT CAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTG AGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATT ACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGC GCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGG AATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACC TGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGT GGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAG AGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTG GCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCA TACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTAT ACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACG TTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGA CAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGAT TTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGA TCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGT ACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTC ATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGT ACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCC TCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTT TAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCC CTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACA TGGGGCGGCCGCTCAAGTAG
[0224] SEQ ID NO: 68 (P_BfP1E6):
GATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATT GGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGT TTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGT AG
[0225] SEQ ID NO: 69 (P_BfP4E5):
GATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATT GGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTGAAATTTAAAG TTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAG TAG
[0226] SEQ ID NO: 70 (Consenso do promotor de fago): GTTAA(n)4-7GTTAA(n)34-38TA(n)2TTTG
[0227] SEQ ID NO: 71 (Consenso do promotor de fago): GTTAAnnnnnnnGTTAAnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnTAnnT
TTG
[0228] SEQ ID NO: 72 (PUL porfirano de NB001):
TAAGGATTGATTCGCTAGCTCAGCAGGTAGAGCACAACACTTTTAATGTT GGGGTCCTGGGTTCGAGCCCCAGGCGGATCACTGAAACAAAAAGCAAAACAAT GAAAACCGCTGATAATCAATCATTATCAGCGGTTTTTCTTTTTATCCATACTGCAA ATTGAAGCAGAATACCGCATTTTACTGGAGGTGAAATAGGTGGACTTAATTTCCA CATAAAAACAAGTCCACCTGATTGGATTATATTTCACTGATTCTCTGCGTTTTGCA TAAAACAAACTCTTTTCAAAACATGTATTTTTACACCATCAAAAAAAGAAGAGTAT GGCAATGCAAAGAAACTATTTTACGGTATTGTTTTTCCTGAAGAAATCAAAGCTG CTTAAAAATGGAGAAGCACCAATCTGTATGCGTATCACAATAAACGGAAAACGT GCAGAGGTACAAATCAAGCGAAGTATAGATGTTACAAAATGGAATACGCAAAAA GAATGCGCGATTGGCAGGGAAAAGAAGTATCAAGAAATAAACCACTATCTTGAT ACGATAAGAACTAAAATCCTTCAAATTCACCGTGAACTTGAGCAGGACGGTAAA CCTATTACAGCAGATATTATAAAAAATATCTATTATGGAGAACACTCTACTCCCAA AATGCTGCTTGAAGTATTCCAGGAACACAATTCGGAATATCGGGAATTAATGAAC AAGGAATATGCCGAAGGTACTGTACTTCGATACGAACGTACAGCAAGATATTTG AAGGAGTTTATCAGTGAACAATATAAACTGGCTGATATTCCATTAAAATCAATCAA CTATGAATTTATAACCAAATTCGAACATTTCATTAAAATACAGAAAAACTGTGCGC AAAATGCGACAGTGAAATATCTGAAAAATTTAAAGAAAATCATCAAAACTGCATT GATAAAGAAGTGGATAACTGATGATCCGTTTGCAGAAATACACTTCAAACAGACC AAGTGTAACCGTGAATTCTTAAACGAAATGGAACTTCGCAAAATCATCAATAAAG ATTTTGATATTCAACGATTACAAACCGTAAGGGACATATTCATCTTCTGTTGTTTC ACCGGTTTGGCTTTCACAGACGTAAAGAATCTGAAAAAGGAACACCTTGTACAG GCTGATAATGGTGAATGGTGGATAAGAAAAGCAAGGGAAAAGACCGATAATATG TGCGACATTCCATTGTTGGATATACCAAGACTTATTTTAGAGAAATATCAGTCAA ATCCAATCTGCAATGAAAAAGGATTATTACTTCCTGTTCCCAGCAACCAACGAAT GAACAGTTATTTGAAAGAAATAGCTGATGTATGTGGTATTCAGAAGAATCTTTCC ACACATATTGCAAGACATACATTTGCATCACTGGCTATTGCAAATAAGGTTTCCT TGGAATCCATTGCCAAAATGTTAGGACACACGGACATTCGTACAACTCGTATTTA TGCCAAAATAATGAATTCTACCATTGCCAATGAAATGAAAGTACTGCAAAACAAG TTCGCAATATAATTTTCAACCATTATTTCATTTCTTACAGCAAATATCGCACTTTG CCACTGACTGTGCAAGGCGGCCCTGTCGGGCTGGTTGGCGGAAAAAAATCATC CTCGCTTCGCTCCGGTATTTTTTTCCGCCAAGCCTTGCACCGGTCATTGGCAAA GAACAGCCGGGCCAGTAAGAAATTGAAATACTGGCTCCACGGAGCCGGTCATG TCTAATTTAAATAAAAGAATATGACTGAAGAAGTTGGAAAGAAGGTATGTGAAGG TACAGTAGCAGACCTCATGAAGGACAAGACCGGAAAACAGACGGTTGTCACGTT GACAAGAAAGAATGCTTACCGAGTGAAGAAAATCAGAGAACAAGGGACGGATG ACGAAGCTGTCCTTTTTCATTTCCGTGAACGCTGTACGGGAATGGGCTCCTATG TACACACAATCGAAGCGGCAGACGGAGAAACAGAACTTCATCCGTCTGAATTTG AAAAATGGGAAGCTGTGGAATTCCTGTATCCCGGCTATCTGGAAGACCTGCTTG ATGCTGCATACAACGCATACAGATGGAGTTCCTTCGAACCTGAAGCAAGGGCG GAAACAGACATCATGCAATATGAAAAACAACTTGTAGAGGATCTGAAACAGATTC CGGAAGAAAAACAGAACGAGTATACCAGTGCATACCATAGCAAGTTCTCTGCCT TGCTGGGCTGTCTCTCACGATGTGCCAGTCCGATGGTGACAGGGCCTGCCAAA TTCAACTGCCAGCGCAACAACAAAGCCTTGGATGCATACCAGAACAGATTTGAT GAATTTCATGATTGGCGTAACCGCTTCAAGGCTGCCATGGAAAGGATGAAAGAG GCTGCCAAACCGGAAGAACAGAAGCAAGAGGAGGCATGGAACCGCCTGAAGC GTGACATTGCAAGCAGCGCACAGACCATTCATGATATTGATACCGGTAAAGCAA GAGGATACAGCCGTGCCTTGTTTGTCAGCAGTATCCTTAATAAAGTAAGCACCT ATGCAGGAAAAGGAGAAGTGGAAATCGTACAGAAAGCGGTGGACTTCATTACAG ACTTCAATGCACAATGCAAAAAACCGGTTATCACTCCGCGGAACCGTTTCTTCCA ACTGCCGGAAATGGCACGCCAGGCCAGACTGAAACTTCAGGAAATCAGAGAAC GGGAAAACCGTGAACTGAAATTTGAAGGCGGAACGCTGGTATGGAACTATGAG GCAGACCGCCTGCAAATCCAGTTTGACAATATTCCGGATGACCAGAGGCGCAA GGAACTGAAATCATACGGTTTCAAATGGTCGCCGAGATACCAGGCATGGCAACG GCAACTTACACAGAATGCCGTATATGCAGTCAAAAGAGTGTTGAACCTTCAAAAC CTATAAGACATGAAAGACCGATTGAAATATGTAATCGATTCCCGCTACTTCGACG GAACATGCCTGACAAGTATGAGTGACGGATTCCATAATGACTATGGTGGGGAAA CAATCGAAGAACTGCGCATACGGGAAAACAATCCCTATCTGAAAGCAGTAACAC CTTCTGATATAGACAAGAAGCTGCGGCTATACAATCAGTCCCTGTCCGAACCGT TCAAGGAAATCACTGAAGAAGAATACTATGACCTGCTGGATGTACTGCCACCCT TGCGCATGAGACAAAACTCGTTCTTTGTAGGAGAACCGTATTACGGAAATATGTA CTCTTTCTGCTTTACTCGTCAAGGAAGATATTTCAAGGGCCTACGCTCCGTACTT ACTCCGCAATCCGAACTGGACAGTCAGATAGACCGTCACATGGAAATCATCAAC CGGAAAGCCGTGATCTCAAAAGAGGAAACAAGTAAAACGGTCACAACCGGAAC CAGACTCATTCCCTATTATTTTTCACTGGACGGAAAACAGCCCGTATTCATCTGC AACCTTGTCATCCAATCAGATTCCAGTCAAGCAAGGACGGACATGGCGAATACC CTGAAAAGTCTTCGCCGGAACCATTATCAGTTCTATAAAGGAAAAGGGCATTAC GAAACTCCGGACGAACTGATAGACCATGTATCAGGAAAGAAGCTCACCCTTGTT TCCGACGGACATTTCTTTCAATATCCTCCCGGCAGGGAATCCGCAACTTTCATC GGACACATCAAGGAGACATCAGAGGAATTTCTTTTCCGGATCTATGACCGTGAA TATTTCCTGTATCTTCTTAAAAGACTGAGGACCGTGAAAAAGGAATCGGCACAG GAACAAATAAATATCAAATCATAACATTCGGGGGAATGCGGTAAAATGACTGCC GTATTCCCTCATAAAAACAATACAAGTATGAACAAATCAAACACTCTATACTGGA AAACAGCCACAGATCCGGCTGAACGCATTGAGGTCAGACTCGTCCTGAACAGTT ATATCGACAATGACAATCTGTATGTAGGACTTGAATCCCGGTCTAAGGAGAATC CGGAATGCTGGGAATCCTACACGGACATCACCGTCAACCTCAATTCTCTTCCCC CGTTCCATGCCTATGTGGACAACCGGGACTGCAACAGACATGTGCATGATTTTC TGACCAGTAACAGAATAGCAGAACCTGCCGGATTTGAATATCAGGGATTCAGAA TGTTCCGCTTCAATCCTGACAGGTTGAAGGAACTCGCACCCGAACAGTTCAAGA CAATCAGCGCCAAACTGCCACCACAGGATGACATGATAAAGGACATCATCTATC AGGAAAGACGTTTCCCTTTGAGAACTGTTCAAGACATTCACGGAATATATCTTGT TTCAAGCAAGGAACTGGAAGAATCTCTGATCGAAGGAGTACGGAACCTGGATGC TGCGGCATATGAACTGCTGGATGGCATCTGCCTGTTCTGCTCCACACAGGAACT GCGCTATCTTACGGATGCAGAACTGATAGAAACAATCTACGCACAATAAAAAGG AGGAACAAATATGAAAACCGGAGACATTGTATTTCTGAGACGTCCCTATAAGGG ATACCGTGCCGTCGAACTGATGGAAAGACTGGAATGCCGCTGGCTGGTCAGGA TTGTCGAGAGCGGTCTTGAACTGGAGGTATATGAAGATGAACTTATATCAGAATT TTAATACAGACAAAGTGTTATGGAAAAATATCAGTTTGCATTCCATTCGGAAATAA TCGGCTATACCTCTCCTCATATCGGTGAGGTCAGAAAAGCCATACACAGAAAAG TGGAAAAGGAAAAGTCTGCCGCCATAAAGAATGATATTGAGCTGCACATGTACA AAGTGCATGACGGCATACCGGTTCTCCTTAACACCTGCTACCTGTACGATGAAA AAGGATGTATGGTACACGGAAGTATCAAGGGAACCAAGGATTATCTGCTTGAGA CATGGAGATACCATACAAACAGACATTCTAAAGGCATCAGTTCCACAAGAATCA GGCCTTGCACGACAAGCAGGGCTTTTTCATTTGTATAACTCTTAAAATCAGAAAT CATGAACCAGACATTACAACTTACAGACTATATTCCACAGAATGTAAGCCTCTAC TACGTGGACTACCGGGATGATCTTGATGAGCATGAAGACATCCAGGAGGAATG CATCCGTTCCAACAAAATGGAAAAACTCTATGAAAAGGCATACGAATGGTATGA GGAACAGGAAAGTTCAAACATGCACGACTATCTGGAGGAGACAAGAAAGAATAT GGAAACGGACAATTTAGCCGGAGAGTTTGAAGAGCATGAAGATGAAATCAGGG AACTTATCTACGACCGGAACGATTCCGACCCGGTAAAGGATATGATACGCAACT CGTCCGTCACTAATTTCTTCTATTCGCTCGGAGTGGAAATCAGCGGATATCTGA CCGGTTGTTCACTGCGGGGAGAATCAGTCGCCATGGCCTGCCATAAGGTACGT CGCGCACTGCATCTGAAAAAGGGGCAGTTTGACGAGAAGATTGAAGAACTGGT AGAGAATGCCACATACGGTGGAGAACTGCGCATCTACTTCAACGCCATGTTTGA CAGGCTCATCAGCAAAGGCCCTGAGAACGATTTCAAGAGCATCCGTTTCCACGG GAATGTAGTGGTGGTCATTGCCGACAGCCGGAACGGTTCCGGACATCATGTAC GGATTCCGCTGGACATCACTTTCCCTTTCCGAAGGGAGAACCTGTTTGTCGATT CACAGGTACACTATTCCTATGCCAATGAAGTCTGCGGCATGACCAATGACTGGT GTGATTCCACAAAATGGGAAACAGGCATGATACCTTTTACCGGATCTGTCCGAA AAAGCCGGATGGCTGAATACAAGAAACAGGAAGCCGCTTATGAGCAGACATTCC GAGACGGGAAATGCACCTTCGGTGACATGAACTACAAACGCCACCGTGACGTG CGGTATTCGAATGAATATCCTGCCGGATGCAGGTGCCCTCATTGCGGTACATTC TGGATTGACTGAAAAAACATTTACCAACCAATAAATTCAAACGATATGAAAATCT GCTGTTCACAAGAGCATTACGACAAGGTCGTACAGTATGCAAAATCAATCAATG ACAAGACACTGGAAAACTGTCTTGAACGTCTAAAACAATGGGAGAAGAACGAGA ACCGTCCATGCGAAATCGAACTCTATTACGATCATGCGCCGTATTCGTTCGGAT TCTGCGAACGTTATCCGGACGGAAATACAGGCATTGTCGGAGGACTGCTGTATC ATGGAAATCCGGACGAATCCTTTGCCGTCACCATGGAACGTTTCCACGGATGGA GCATACATACCTGACATATATGCGACAGTCTGTATTGGGGAGCCTCATGCAATA TGGGGTTCCCTTTTTTTATGCCGCAGACATGATGACAGCATCCTCATTTCTTGCT GCAAAAATAGCTGTTTGCCGCGCAACTCCCGCAAGGCGGCCCTGCCGGGCTGG TTGTCTGGAAAAAAATCATCCTCGCTTCGCTCCGGTATTTTTTTCCGCCAAGCCT TGCAGGGATGCGGGCAAACAGACAACAGGGACAACAAGAAATAAGAATGCCTG TACCTTACAGGCAGACAATGTATAACAATAAATATCAGAAGTCATGATTACAGAC CAGAAGACACAGAACAGGCTTCACGCGGATACCGGAACGGAACTGTTCTCCAT CAGACAAAGGAAGGAAGCCGTCACAAGGATGCTGGACATTCTGAAAGAGACTC CGGAATACCTGCAGGTTATGAACCATATACCGGCTTATGCCATGGATGACGATA CGTCAGAATGGTGGAAATCGGAAGAATCGGAAAATTTCATGAACTCACTCCTGG AAGTGATGGAAAGCTATACTCCGGACGGATACAGGTTCGGACCGAAATCCGGC ACGACTGACCTTTACGGCTACTGGGAAAGCAAGACCGGGCGGACAACCCTCTT CCATCTGCTTTTCAGTCTGGAAAGCGGATATGAATGGGGAAAAGGTCTTTCCCA TGAGAAAACGGACGCATTCTACAAGGAAATAAAAGAGAAATTTCATGGAGAAGG ATTCGACACGGACAGAACCGGCTGTACATCACAGGCCATGTATCTTGTAAAAGG AAAAACACGCCTGTACGTGCATCCGATGGAAATAAGCGGCTACTGTGAAACACT GCATATTCCACAGATTACAGCCATACTGAAAAAAGGAGGCCGTACATTCCGTCT TGTAAAGGATACGATAGCGGAAGAGGTGTATTCCTTCACCGATGAAGAAGAACT GGAATATTACCGTGCCAGATACGGAACGTGCATCCACCGGAATATACTGGATGC CTTCAGCAACCGCCACGCAGGGAAAGAGGACATACTTTCCATGATGGCATCAC GGATAAATGTGGCTACGACATCACATCTTTACGGTATCGGATATGATTCGCCTG CATACAGGTTTGTGCATGAGGCATACGACAGACTGGTAAACAATGGAAAGCTGA AGGAGAATGTCCGGGAAATCGGTTGCTGCAACATCATAATGGCCATTTCAAATA CCAACGCAATATGAGACTGAATTACAATGACATGCTGCTTCTGGCAATATGGGA ATACAACAGGAGACAGGACGAGGATCTGACCCTGGAACTGTTTCAGGAAACATT CGGACAGGTTCCCGGCGCACATTTCCATGACAAATGGGTGCATTATTACAACAA GAACCTGCTGATGATGGCCGCCTATTTCAGGGGTGAGGAAGAAAACGGCCAGA AATTCTGTGATATGATCACCCGACAGGTTGAACGCTATACACAAAACAGGAGGA GAACAGGATGAATACAAAGATACGATATGACCTTGACAGTCTTGAACTGGCAAA CGGTGACTTCGGGTATCCCATTACAGAAAAGGAAGTACGGAAAGTGAACCGTAT GCTGGAACTGATGGAGAATGTCCGAAGCAGGCAGATGTGCCCGACAGAAGGAG ACTGCGTGGAATTTGTCTCACGTTCTGGTGACTATTTCGGAAAAGCTCATATAGA ACGGATAACAGGAAAATATGCGGATATATGCCTGATACCGGAAACGGTATTCTG TTTTGATGACATGGGAAAAGCCGCCTATGATACCACCGGAAGTCCCTGGACGCA GGTCAATATCCGGAACATGAAACCCGCAGGTTCTGAAATCCGCATATTCAGAAC ATGGGGATTCGGGAAGCGCAGCAATACGGGCAGTCTCAGGTTCGATGCTCCGG TCAGGAAATGGGAATACAGAGAACCGAATCCGTTATATGACGGTTACACCACCC GTAACTGGTTCCGCTATCATATCATGAAACACCGGGACAGGGAAAGGACAGGC GAATACACCTTCCGCAGCGATTCATTCACGCTGTACAGCCGGAGCGAGCTGGA CGAGCTGGCCGCAATCCTGAAAGGCAGACTCTACAAGGGAATCCTGCCTGACT CTCTTGTACTTTGGGGATACCGCATGGATATTAAGGAAATATCACGTGAACAGT GGAACGGTATGGGACAGCACGGACAAATCCGCATGAAATTCATGGGATACGGT CCGGTCAGAATCCACACGGACAATGAAAACCATACCGTAACAGTATACAGAATC AACGACATATTGTCTTCAACTATCAGAATTTTCATATTTTTTCAGTTCTTTTTTTGT TTCTTCTATTAATATTTTAAGCCACTCCATGATTTGTATTGCATGTTCATGAACAG TTTCATTTTGGCTATCACTGTCGTGTAGTAGCCTTTGAAAATCACGTAAAATATTG TCTTTCCCAAGCATCTCCCATACAGGCATCATCCGGTGGATTATTTTTCTCATGG TCTCACGGTCGGTTATCCTGTCAGCAGATTCCATCTCCTCCAGTTCTTTTTCAGA TTCCATAACAACGAGAGAAAGCATATGACTATAATCATCCGTATTCTCCAGTAAA CTGGAAAAATCGAATTCTCCGGAAACTGAAACTTGTGTACGAGATATAATGGTG GATAAAAAAGCAAGCAGTCCGTGGATATTGAACGGTTTATGAATACAGCCTACA AATCCTTCTTTTTCATAAATTCCGGAATTTCCGTCACCACGGGCAGTCATGACTG CTACTGGAACAGTTCTAGAATTGCCGATGTCCGAATTGCGAAGCAATCTTAACAA ACCGAATCCGTCAGTATCAGGCATTTGTACATCTGTCAAGATCAAATCATATTCA GAATTTTCAAGAGCGGCCACTACTTCACGTGCATTCTTACAGGTTTTACAGGATA TACCTTTGCGCCCGAGCATATCTTCCGCTATTTTCAGTTGTATAGGATCATCGTC CACTACAAGAACATTCTTAGGCAATATAGTTATTGTATTATGGTCCGATTTGTCTT CCTCAACTAACTCATCCGTTTCAGGCAAAGAAAGTTCCAGTCTGAACATGCTTCC TTTACCGAGTACACTTTCTACATCCATTTTTCCTTCCAAAACCTTAATTAATCCTTT GGTAAGGAAAAGTCCCAAACCAAACCCTTCAGAATTGACATTCTGTGCGGCACG CTCAAATGGAGCAAATATTCTTTTCAGTGTTTCCTCATCCATACCGATACCAGTA TCCCTTATTTCAATACGAAGTTTTCCTTCTGAATATTCTGAATGGAAATTGACGTT ACCCCTGGAAGTAAACTTAATAGCGTTTGTAAGTAGATTGGCTAAAACCTGTTCA AGTTTGTCCGCATCACCTTTTACTATTACATTTGATCCTTTATGTTCAGAATATAA AATCAGACCTTTTGAAGTCGCTTTACGAGAAAACTCATCTGAAATTCGTTGCAAG AAACGGTCAAGATAAAATGGTGTGTCGTTACGCAAATTACCGGCTTCATTGATTC GGTAAGCATCCATCAAATCATTAACCAGATGTAAAACGTGTCGACAAGAATGAC GGATGTCATCTAAATATTTTTCGCGCTTCCTCTTTTCACGCGTTTCAGATACCAA ATCTGCACAGTTATGGATATTACCAAGTGGACCTCTAATATCATGAGAAACTGTC AGGATGATTTTCTTACGCATATCAAGCAAATTCTCGTTTTCTTGAATAGCTTGTTG TAATTTAAATTTAATTATTTCTTCCTTACGTAAATCTGATTGTATAATTAAAAATGA AATTAATATTATAAAAACCGCAATACTCATCATTACGATAAATAATCGAAAGGATT CTTGTTTGACTTCCGTTACCTCTAAGTTTCGTTCTATAAATGACAGCTGTACCTG ATTATCTAAAAAAGATACAAAATCATATAATTTTTGATTTAACAGCCTATTCTGCAA ACGCAAGCTATCCACATAAGTTTCTATCTGATTGTTTCGCATATCTATGACAGAA ACCAATCTATTATTAAAATTCTGTATTTCATTAGTTATATAGGGGACTTGTATCGT CTCCTTCTTTCCGAATAATCCGGCAATTCCTTTCTTTTTCTGAGTTATTGTCTTCA CTTTTACTGTTTGAGTAGCTATTACAGGCAATTCATTAGTAAGAATACTATCAGAT TTATTCGCAAATTGGACTGCTTTCATTATTTGAAACAAGTGCATTTCTTTCGTTTT AAGCAATTCCCGTAAAGAATCAATTTGAACTGGACATAAAAAATCACAACTCCTT AATTTTATTTCAAGTAGAACACTATCTGTTTTAAAACGTTGATTATGAAATATGTTA TAATCAGACTCATCCCATACTATAACTGATTCGCCTAAAGTTGCCAACTTAGTAA TATACAAATGAACTTTATTAGTATTCTCATAAGCTTCATTAATTTGAATTATCAGAT TCTCAAGTTCTTTCAACCGGCAACGTTCATTTATCATTACAGTAACCATACTTAAG ACTATAAATCCTGTAATAAAATATCCAATAAATAGTCTTTTGCGTAATAATGAAGT CATCAGGAACATTCTATTGATTTATTTGACATCATAATTCTATATATTTAACTAGTC ATAGTATATATCATTCTCAAATATTTATTTCAAATTCAAGCAATAAAATAAAAAAAC ACTTCATATTACAACTGAACTCTTTTATGAAAAAGTTGAATATATGAAGTGTTTTTT TATTACGATATAAACTATAAAATCCTATTCTTCGGGAACTGGTGTATAAACCCTTA TCCAGTCCACCAGGAAGGTGTGGTCTTCCACATTTTTCAGTTCCTCATCCGTAG GGCTTAAACCTTTAACGGCTCTCCAGCTTTGGTCTTCCATATTTATTATGATGTC CATGTCTTTTACCAGACCTGTACCACCAGTGTAGTTGTTGGGGTCGATAATATCC TTGCCGCTTACGGTTCTGACAAGTTCTCCATCTACATAATATTCAAGTGTGAAAG GGTCTTTCCAGAACACTCCTACACGATGAAAATCGTCGCGCCACAATGTTCCCT TGTCATCCTTATACCATGAGCCAAGATCTTTCGGCTGATAATCCTTGAATGGCTG GCGGATGAATATGTGATGGCTCAGGTGAAGTCTGTCGGCACCGTAACCTCCGC CGTCTCTGTCGCCGCCGTATGCTTCTATGATGTCGATTTCCTGAGTATCGTCAG GGCTGAGCATCCATACATCGGATGCCATGGTTGAATTTGAAAGTTTTGCGTATG CCTCTACATAAACCGGATACTTTACACGTGTCTTCGATGTGATACATCCCGTATA GGTTCCCGGCAGTTCCTTTGTGTTGGGTCCGCTTACAACTTTCTTCATGGGGAC ATCTTCAGGACGGCTGGCTCTTATTTTAAGGTATCCGTCGGAAACGGAAACATG GTCTCTCTGCCATATTGTAGGAGCAGGTCCTGTCCAATGATTATGATAGAAATCG GTCCATTTGGCATAGAACTCTTTTCCTTTATCCTTTTCGTCGGCAACATAATTAAA GTCGTCCGACTGTGGATGGAGTTTCCACACCATACCGTCGCCGGCATCAGCGG GTACAGGATAGATATCCCACTCGTACGATTTATTATTGAAATCTTCTGCTGCACA GGCTATTTGCAGCGATGCTAAACAAATGGTAAACAGTTTTCTCATCGTGGTATCT TAGTTTAAGTTATAATAATTATTTTCGTTCTTTTGATTCACCTTTAGCGGTATGTGT CTGCAATGTCCAGGTAGAAAATCTCATTATGCTCTGATAGTCTGAACTGTTGTAT ATATGAGTAAGACCCCATCTCAATATTTCGGTAGGTTCTTTTTCGGCATCTGCAC TGCGGTTCAGGCCAATGGCGTGTGGCGCGCCTTTTACTACTGACATTATTTCAA AGTTTATTCCGTCAGGCGACCACTGGAGTGTGTTCTTTTCAGGACCGTCGGTGG TGATAAGTGAAGCTATACCTCCTTTGTAAGGCCATACGCAAACTTCATGCCCGCT GTTTGAAATAGGATTATATTCCGATTTCACATACGGACCCATAGGATTTTCCGCA ATAGCCACTCCGTGTTTGATTTCACGGCCGCCCCATGTTATTTCTTCTCCCATAC GTTCGCCTTTGTAGTACATATAGAACTTACCTTTATAAGGTATTATACACGGGTC GTGTACCTTATGACTGTCGAAATCACCTTTCGACACTACCTTGAATCTGTTATCC TCATCGCCTTCCCATTCGCCGGTATTAGAAGGTTCCAGTACAGGCTTGTCTGTC TTGATCCACGGTCCTTCAGGGGAATCAGCACATGCCATACCGATAGTATTCTTTA CACGGACTGTGTAAGGGGATTTTACCGCCTGATAGCAAAGATAATACTTTCCTTT CCATTCCATCACCTCAGGAGTGAAGACTGAACGGTCGTCGTAAGCACCTTTTTC ACCACGTTTCACTGCAATTCCCTGTTCCTTCCATGTCCATCCGTCTTTTGATGTG GCATACCATATATCACATCTGTCCCATGGGAAAACCTTATCTTTCTCTATATCTCC AGCAAATCCTTGGGTAGGTCCATAGCTCTTTGAATACCATACATAATATGTATTA CCTATTTTCAGCATTGCACTCGGGTCTCTTCTTACTACGCCCTCTTCATAAGCAA GATCACCTTTAAGTGGTTCCATCTTATACTCAAAGAACCATTTATTGTCGTGATTT TCCCATTTCATGGCACGTTTCATAGCTGCACTTAACTTATTTCCCTTAGGTATTCC CAATGAATCGGCCTTACGCTCATCATAATTCTGAGTGTCGTCAACGGCAATAGT CTGTGTATTGCCTGTATTTCCGCATGCTGCCAATAGCGACATCATGCCGGCTGC AAGAATAATTTTTCTCATACTAGACTTTATTTTATATTAATTGTTAGTTTATTCGAG TGTAATTCACTTGTTTCTGCACTGATATTCAGTACCGATGATTTTTCTGTCGACTG AAGCATCAGCATACATCTTCCCTGATATGTCATAATATCCTTACTTTGATATGGA GAAACGTTCTTCACGTTTCCATTGTCTATACCAAGCAGACGGTACTCTCCATCAA TGTTGAACTTAAGCATCTGTTCTGTTGTCTTTACAGGATTACCTTTTTTGTCTGTT AGCTGAGCTGTGACATGCAGAACATCCTTTCCATTTGCTGCGATACTTTGTTTGT CAACCGTCAGCAATATCGAATGTTCTTTGCCTGAAGTCCTTATAGCTGTAGTGGT ATTACCTAACTTATTTTTTCCTTTTGCGGTAATAGTGCCAGGCTTGTACTGAACTG CCCATTTATAGATATGATCCTCAAAATCGTCTATATACTTCTTTCCCATCGACTTA CCGTTAACGAAAAGTTCCACTTCATCACAATTGGAATATATCTCTACTATTACCG AGTCACCTTTCTGATAATTCCAGTGAGAGTTTACATCATCCCAAACCCATAATTTT CTATCCCATTCATGTCCTTTCTTATCAGTAAATCCATCTTTTACATGGAGATACGA AGATTTGTCTGTAGTCTGTGAATATATAGCAATAAAAGGCTTGTCTGTCCACAAT GATTTCATCATGTCGTACGAAGGCTTCACATAGCCGCACATATCCAGGAGACCA CATCCTATCGACTTTTGAGGCCATTTTGAAAGACGGCTTTCACTTTCTCCCAGAT AATCGACTCCTGTCCATATAAACATACCCGGAACGAAATCCCTTTCAATCACCGC CTTCCATTCGTGCCACTGACCGAGATTTTCTGTACCCATTATAGGCTTGTCAGGA TAATTCTTCTTAGCATAATCATACATCACGCGACGGTAGCTGAAGCCTGCCACAT CGAGCGCGTCGATATATCCTGACTCAAAGCTTATGGAAGGCAGGATGCAGTTG GCGGTAACTACACGTGTGGTGTCCATCTGGCGTGTCCATGCAGCTAATTTTTGC GCTGTACGGCCAATGTCGTATGCATGTTTAGGCTGGATTTTCCACATTTCTCTGA TTTTTTCTTTAGAGTATGGAGGCTGATTCCAGAAATAATTACCGTTGGAATCGGC ACCGAAGAAACCTGTCGCCTCGCGGCATCCGGTATAAGTCCATTCTATTTCATT ACCTATACTCCACTGGAAGATACAGGCATGATTACGGCTTCTCCTCATTACGTTT TTCAAATCTCTTTCTGCCCATTCCTGGAAATGCTCGCAATAGCCATGCGTAGGAT AGTCTTCTACAGTTTCCTTCATATTGAGTCTTTTATCTTTGGGATAATCCCACTCA TCGAAGAATTCTTCCTGAACCAGAAGACCTATCTCATCGCACAAAGACAGAAACT CTTCCGCTCCCGGATTGTGCGAGAGGCGGATGGCATTGCATCCTCCTTCCTTTA GGGTTTTCAGACGCCGGTACCACACATCGCGTATCATTGCCGCGCCAACCATTC CGGCATCATGGTGCAGGCATACTCCTTTTATCTTCATGTTTTTCCCGTTAAGGAA GAAACCTTTGTCTGCATCAAAACGGAATGTCCGTATGCCGAACCTGACAGTGTT TTCAGAAATTACTTCATCGCCATTCTTGATGCGTGTCTCGGCTGTATAGAGGACA GGTGTATCGACGCTCCACAAATCAGGCTGTTTAATCTCAGATACGATGTCGATA ATTTTCTCCTCACCAGCATTCAGTTTTATACTGAAGACCTCAAAGGCTGCGATAT TGCCTTTATTATCCTTATATACTACCTCAACAACTGCAGCTCTGGGTTCGGAGTA GCTGTTGCACACGGTAACCTGGTTGTTTACTTTAGCATATTTATCAGTAACCACG GGAGTAGTGACAAATGTTCCCCAAACCGGAATATGCAGTCTGTCGGTTACAATC ATTTTCACATCCCTGTATATACCTGAACCGGTGTACCATCTGCTGTCGGCATAAT GGCTGTGGTCGACCCTTACAGTCATACGGTTATCCTCATTGGGATTGAGATAGT CTGTGACATCAAAATAAAAAGGAGCATATCCCGAAGGATGATATCCAAGCTTTTT GCCATTTATCCAATACTCAGAATTATTATATACTCCATCGAACACTATATAGCATT TCTGATTTGCACTGATTGTTGTGGGAAATGATTTGCTATACCATCCTATTCCTCC CTGAAGGAAAGCTACACATCCTTCACCCGAAATGGAATCGTAAGGTAAACCAAC ACTCCAGTCATGTGGCAGGTTCACTTTCTTCCATTCATCACCAGGGACATAAGAA GTATATGAATAATGAGCAGAATCTTTCAGTACGAATTTCCAATCTTTATTGAAATC AACATTTGAATCAGATGCTGAAACCTTTAGGGTTGATAATAGGATTATTAAAGCT AAAAGATTTTTATTTCTCATAATCTTAGGTTTTACATGTTTTTTGATGTCACAAAAC TATATCTTTCACTTATAATATATGAGGGGGATATTAATGTGATATAGGGTGGGAA ATCAGAATTTTACATCTGCCCTGTATTCCACCGTCACCTACAACCTTGACAAAGG ATGTTCCTTTCTTCCCTCTTATGGTTCTCAGGACAAACAGACACTTTCCGTTATAT GTCCTTACACTATTGTTTATGACGTTGATGTTCAAATCTTCTATCGAAGGCGATC CATTGTCGAGTCCGGCAAGTTCAAGCTTGTCGTCGAGGATTATCCTCACATCCG AAGGTATATCGACTACTGTGTTTCCTTCTTTATCTTCAATGGATACTTCTACATGG ATAAGGTCATAACCGTTGTCGGTAGCTGTTTTGCGGTCGCAGTTCAGTGCCAGA CGGCACGGCTTGCCGCTTGTGGACAAAGTGTCTTTCGACAATATTCTGTCGCCG TCCTTGCCTACCGCAAGGAGTGTTCCTTCCTTGTATGCCACCTTCCACATCAGTA TATTATGCTCCATGAAATCGCTGCGTTTCTTTGTTCCCAACGATTTGCCGTTCAG AAACAGTTCCACTTCTGGGGCGTTGGTATATACCTGCACCAGTATGTCCTCGTC CCTGCGGTACTTCCATTTATCGCGTGTGTCGTACCACTCCCAGCGTCTGATCCA TCCCGGGCGTGGAGTGTAGGTGAAACTTCCGTCAGTATCCATCTTGAACTCGCT TTCCTTTTCAGGTATTGTTACAATATGGGTTTTCGGTGTGTCTTTCCACAGACATT CAAAGAAATGGCCACGCGCTGTCTTGTTGCCCACGAAATCGAAGAAAGAACAGT CTCCACCCCTTGCAGGCCATGGGCCGTTCTCGCCAAGATAGTCGAATCCTGTC CACACGAAGATGCCCGCTATGTACTTCTTGTCGGCCACGGCTGTCCATTCAAAG AGCTGACCAACATTCTCCGAACCGATAATAGGCTGATATGGATATAGCTTATGGT CGATTTCATAATATTTGTCTTTATAGTTATATCCCACTACATCAAGAACGTCTGTA TATCCGGAGAGACGCGAAACTGACGGAACAACGACTCCTGAAGAGACGGGACG GGTAGTGTCCACATCCTTAACCCAACCGGCAAGGACAGCGGCTGTTTCAGCCA AATCGTCTTTTCCTCCTGACAGACGGTTGAACTCTTTCAGTATAGACTTGTTGTC TGTTTCCGGGTCGCCCGTATGGATAAGACCCTTGAACCCTTTATTGTCTTTGCTC GATGCCCAGTAATATGGATAGGTCCATTCTATTTCATTGCCTATACTCCAGAGTA TCACGCAAGGATGATTTCTGTCTCGCCTGATGAACGACTTGAGGTCGTGCTCGG CATGCGTATCGAAGTATCTGGTATATCCTATTGATATGCTGTCGGGCGCATCTTC CTTAGCTCGCTCAGTAATCCACTTTTTCTTTGCCACCTTCCATTCGTCGATAAATT CATTCATTACAAGAAGTCCCAGACTGTCGCACATTTCCAGCAGACTTTCCGAATG CGGATTATGGGCTGTACGTATGGCATTGCAGCCTATGGAACGAAGTTTCAGAAG GCGTCGCAACAGGGCATCATCGTATGCGGCAACACCCATACATCCCAAGTCGT GGTGTATGTTCACTCCTTTTATTTTTACTGATTTTCCGTTTAGAAGGAAGCCTTCA TCCGCATCGAATTTAATGTCGCGGATACCAAATTTTGTTGTTTTCTTATCCATCAC ATATCCGTCAGAAGCAATCAGAGTAGTATGAAGCTCATACATCGAAGGCGTTTC AAGACTCCAGAGATGACAATTCTCCAGTTCAACAGATGCAGTGAACTCATTGAAA TCGCCTTTCAGGGCAACAAAATCATCGGAAACAGAAGCTATTGTCTTGCCGTCG TACACTACTTCGTGCTTCACGGTGACTCCTTTTACACCTGTTCCAGCATTCTTCA CCTCGCATACCACATTCACCATCGAACGGTTGCCTACCTGTGGTGTGGTAACGA ATATTCCGTCTGAAGGAATATAGAGCTCGTTTCTTAGAATAAGACTCACATTCCT GTATATACCGGCACCGACATACCATCTGCTATCGGCATACGCTCTTCTGTCAAC GCAGACAGTTATTGTATTCATCGAACCTTTTGGTTTCAGATATTGAGTAAGTTCAT ATTCAAATCCCACATATCCGTTAGGACGGAATCCCAACATATGCCCGTTTATCCA AACCTTTGAGTTATTATATACACCTTCGAAATGAATGAACACTTTTTTCCCATTCA TATCATCCGAGGTGAGAAAATTCTTCATGTAAATCCCCACACCGCCAGACAGAA AACCATTGCTTCCGGCTGTCTGAGTCTTGGTATATCCTTCGCTGATACTCCAGTC ATGAGGCAGACACACATCCTCCCACTTTATATCTGGACTCAGGAACAAAGTGTC CTGAGGCACGAAACCTGCTGGTTTGCTGAATTTCCAATCGAAGTTGAAATCCAC TTTAGTGGAGGTTCCGGCATAACAGAATCCGGACAGAAAGATAGTTAAGACTGT GATAATGTTTTTTATGGTCATATCGATTTTCAGATTAATATTAATGACAAAAATAAT TTCAAAAGTGTAAAAACAAAAAAACTCTCCATTTATATTTCAGATATCAACGGAGA GTTTCATCATTAAAAAAAATAAAACATTTTATAAAGTTACTCCTTGCTTAAGGATA GCTATTTCCCGGTATCCCTTCTTTTCGTTCAGTGCCTGCTTTCCGCTTGCCACTT CCACCACAAAGTCTATAAAACGTCTGCTTAAAGATTCCATGCTTTCTCCCTCTAC CAGAGTTCCGGCATTGAAATCAATCCACGTATGTTTCTGTTCATAAAGCGGAGT GTTGGTCGAAACCTTCACGGTTGGAACGAATGTTCCGAACGGTGTTCCGCGGC CTGTTGTGAACAGCACGATATGGCATCCGGCAGAAGCAAGAGCCGTACTTGCC ACTAGGTCGTTGCCTGGTGCGCTCAACAGGTTAAGTCCGTGTGTTGTGACACG GTCGCCATATTTCAGAACATCCTCCACCATCGAGCTTCCCGACTTCTGTGTACAT CCCAATGATTTCTCCTCAAGCGTGGAAATACCTCCCGCCTTGTTTCCCGGTGAA GGATTTTCATATATTGGCTGGTCGTTGCGGATGAAGTAGTTCTTGAAGTCGTTTA TCATGGCCACTGTGTCGTCGAATATCTCCTTCGTGCGGCAACGGTTCATGAGCA GTGTCTCGGCTCCGAACATTTCAGGTACCTCCGTGAGGACTGTTGTCCCACCCT GGGCAACAAGATAGTCAGAGAACACCCCAAGCATCGGATTGGCCGTGATACCG GACAGTCCATCAGACCCGCCGCACTTGAGTCCTATACGCAGTTTTGACAGGGG GACATCAGTCCGCTTGTCTTCCCTGGCTATGGCATACATCTCACGGAGAAGTTT CATACCCTCTTCTATCTCATCATCTACTTTCTGAGAAACAAGGAAACGGATCCTT TGGGTATCATAGTCACCTATAAACTCACGAAAGGCATCAGGCTGGTTGTTCTCA CAGCCAAGACCTACGACAAGGACAGCTCCGGCATTGGGATGAAGGACCATGTC ACGCAATATCTTACGGGTGTTCTCATGGTCGTCACCCAACTGCGAGCATCCGTA GTTATGAGGGAAAGATATAATGGAGTCAACCCCCTCGCAACCTGTTTCCTTGCG AAGCTGCTCGGCCAACTGGTTTACTATTCCGTTCACGCAACCCACCGTAGGGAT AATCCATATCTCATTACGTATGCCGGCTTCTCCGTTAGCACGCAAATACCCTTTG AATGTATGGTTCTCGTTCGTGAATGTCTGTTTCTCGAACTTCGGAGTGTAAGTGT ATGTACTCAGACCGGAAAGGTTCGTCTTGACGGTTTTCTCGTTCAGCAGATGTC CTTTCCTGACTTCCTTTACAGCGTGCGATATGGGGAAACCGTATTTTATCACCAT ATCACCTTCTGCAAAATCCTTCAGGGCAATCTTATGACCGGCAGGTATATCCTCC ATTAATTCTATGGAATTGCCGTTCACCTCTATTACAGTCCCTTTGGACAATGGGT GCAGTGCCACAGCCACATTGTCCGCAGGGTTTATCTGGATATATTCAGTCATAA CAAACTAACATTTATAAATTGAAGAATACAGGTAGAAGTATCAACCTACAAGGTC TTTTACTGTCTGAAGCATTCCTTCGCTCTGGATTTTGTTGATATAGTAAATTACAC GGTCTGCCAGTCCCGAGATAGTATTAAGGTCTTCACCCCAAATGGAAGTATCGG CGAGAACTGTCTTCACAAGATTTTCTACCGAGCCATCGTTCCACAAACTTGTAAG CATCGCCATGATTTCCTGTGCATCGTTAGGAACTATCTCTACACCATCGGCACG CTTTCCACCTTTGTAGTATACTATGATGGCTGCAAGACCGAGTACAAGTCCTTCA GGAAGCACACCCTTACGTTTCAGATATTCCTTCACTCCTGGAAGGTCGCGTGTG GCATACTTAGGGAATGAGTTAAGCATGATTGATGTTACCTGATGGTCTACGAAA GGATTATTGAAACGTTCCAGGACATCATCGGCAAACTTCTTGAGTTCCTCTTTCG GCAGGTTGAGGGTCTCCATCAGCTCGTCGAACATCACACGTTTGATGAACTTGC CTATCACCTCATGTTGGCATGCGTCTCTCACGATATTGACGCCCGAAAGGAATG CCACCGGCGACAATACAGTGTGAGGACCGTTCAGCAGAGTAACCTTGCGTTCAT GATAAGGCTCCTCCGACGGGACGAACAGAACGTTCAGTCCCGCCTTGTTTGCA GGAAATTCTTCGGCAACCGATTCCGGTGCTTCGATAACCCACAGATGAAAAGCC TCGCCCTGTACAACTAAATTGTCATCAAAGTATAGTTTAGTTTTTATGTTGTCTAT GTCTTTACGAGGGAAACCCGGTACGATACGGTCCACCAGTGTGGCATATACACC ACATGCAGTTTCAAACCATGACTTGAACTCTTCGCCAAGGTTCCACAATTCAATA TACTGATAGATTGTTTCCTTCAGTTTGTGACCGTTGAGGAAGATAAGCTCGCATG GGAAGATGATGAGTCCTTTCGACTTGTCACCGTTGAAATGTTTGAATCTGTGATA AAGCAACTGTGTCAGCTTGCCCGGATAAGAGCTTGCAGGAGCATCCTCAAGCTT GCACGACGGATCGAAGTTGATACCGGCCTCAGTAGTGTTCGAGATTACGAATCT CATATCAGGCTGTTCCGCCAGTGCCATGAAGTCATTATACTGGCTGTATGGATT CAGCGCGCGGCTGATGACATCAATCATTCTGAATGAGTTCACCACCTCGCCATT GTTCAGTCCCTGAAGATTGACATGATACAGACAGTCCTGGGCATTGAGGGCATC AACCATACCTTTTTCTATAGGCTGCACCACAACAACACTGCTGTTGAAATCTGTC TTTTCATTCATATTCGAGATAATCCAGTCGACAAACGCACGAAGGAAATTACCTT CGCCAAACTGTATGATACGTTCCGGACGTACTGCCTTTACTGCAGTCTTACTATT TAAAGCTTTCATTGTAATGCCAAAAAATTAAAATTGATAAGATTAAAATTCAACCA ACATTCTGAATACCTTACCTGGATTTTCCGACCATTTCTGCAGAGCCTCGCCTGC CTCTTCAGGTTTCACTACGGCAGAGATAAGTTCGTTCATCGGGCAGTTGCCATT CTGAAGATAATGTATCACGGCACGGAAATCCTCAGGCATTGCATTGCGCGAACC GCGTATGTCGAGTTCCTTCTGGACAAAATATTTTGTCTGGAAAGCCACTTCACTC TTGGCATAGCCGATACATGCCACACGGCCTGTGAAACCTACAATGTCGATGGCA GTAACATATGTGATAGGACTACCCACAGCCTCTATCACCACATCAGCCATATAG CCGTCAGTAAGTTCCCTTACTCTTTCCACCACATTTTCAGTCTTCGAATTGATAA CCATCGAAGCACCCAGGCGTTTTGCCAGTTCAAGCTTCTCATCGTCAATATCCA ATGCTATTACCCTTGCGCCACGAAGCGATGCTCTTACTATGGCGCCAAGTCCAA TCATTCCGCAACCAATCACGGCCACAGTATCAATGTCAGTTACCTGAGCTCTCG ACACGGCATGGAAACCTACGCTCATAGGCTCAATCAGCGCACATTCCTTATCCG AAAGACCGGCAGCCGGAATAACCTTTGTCCAAGGGAGGACAAGGAACTCCTGC ATAGAACCGTTACGCTGAACACCCAAAGTCTCGTTGTGTTCGCAGGCATTCACA CGTCCGTTGCGGCATGAAGCACACTTTCCGCAGTTGGTATATGGATTTACTGTC ACGTTCATTCCCTTCTCGAAACCGACAGGAACGCCTTCGCCTATTTCCTCTATCA CAGCACCCACTTCATGTCCCGGGATGACAGGCATCTTCACCATAGGATTTCTTC CCAGGTAAGTATTAAGGTCGGAACCACAGAATCCGACATATTTGATACGAAGTA AAATTTCTCCGGCTCCAAGTGTTGGTTTAACTATATCAGCTACTTGAACCTTTCC GGCTTCAGTAATTTGTACAGCTTTCATAATCTATGTATTTATTTAAATTTGTTATTG TATTATTTTGATGTTGCATTAATTCAATGTTGTTTTTTCTCTATCTTATATCCTCTC CAGCCATAATATGCCGTAAAGAAGAAACATATCAGAGGTATTACATATGCCACCT GATAGAAGTCCGCGTTATGATTCATCACAAATGCGGTGAACTGAGGGATGCACG CATTACCTATAATAGCCATCACAAGGAATGCCGAACCACTCTTTGTGTCCTCGCC AAGGTCGCGTAGTGCAAGTGAGAACTGGGTTGGATACATTATCGACATGAAGAA CGACACTGCAAGCATGGCATAAAGTCCTGTCATACCACCGAACATGATAATTAC TCCACACAGTATGATATTTACTATAGCGTATGTAAGCAGCATATCCTGAGGTCTG AATTTCGACATTAGCATAGTACCTATCCATCTGCCGCCAAGGAAAGCCAGCATAT ACAGTCCGAAGAATGTGGTCGCCTCATCCTCCGACAGACCTGCATACATGCAGC AGTAAACTAGGAACAGGCTGTTGATGGCTGTCTGCCCTCCGTTATAGAAGAACT GTGCGATAACTCCCCATCTCAGGTGTTTGCGTTTCAACACTGCAAAATTGATAAG CTTGCCCTTCTCGCCGTGCGATTCCTCCTTGTCAATATCAGGCAACTTATACAGT GCAAACACCACAGCAAGAATAATCAGCAGGACTGCAAGAACCAGATAAGGCATC TTCATGGAGTCTGTCTCCATCTGAATAAATCCGTCCCAACCTCCGGGAAAGTCG GCAGGCAGAGTCTCGCGAGTATAGTTCTGTCCGGTAAGTATAAGCTTACTCAGA AACATTGCGGATATGAAAGCACCAAGACCGTTGAACGACTGTGCAAGATTCAGT CTTCTTGAAGCCGTATCGTGTGTACCCAGAGCTGTCACATACGGATTGGCAGCA GTTTCGAGGAAGCACATTCCCGTTGCCATGATGAAGAAGATTACAAGATATGCC CAGTATTCCTTTATCTCGGCTGCAGGGAAGAAAAGCAGACCACCGATGGCTGCA AGAATGAGACCGACAATTATACCCGACTTATAGCTGAAACGTTTCATGAACATTG CTATCGGTATGGGAAACAGGAAGTAGGCCAGCCAATAGGCAGCTTCAGTGAAC GAGGCCTCAAAAGCATTCAGTTCACAGGTTTTCATCAACTGCCTGATCATTGTAG GCAATAGATTACTGCTGATAGCCCACATGAAGAACAAGCTGAATATCAGTAAAA GCGGTATAAAATATTTGTTTTTCATTCTGACATGTTTTTAATATAAGGTAACTCAG GCAGATTCTTGAAACCGTAAAAGGCTTTCGCGTTCTCGCCCAAGAAAAGTTTTTT GCTTCTCTCTTCCAATTCTTTTGATTTAATCACAAAGTCGTACGACATCTTGTAGG TAATGGCTGTGATTGTGCGTGGATAGTCGGAACCCCACATCAGTTTCTCGAAGC CAACAAGGTCGGCAGCTTCGTTGATGGCTCTGACAGCGCTGCGGAACGGATAG AACTCGTCATTGAACAGCCAAGTGATACCGCCCGACTCAATCATCACATTCTTAT GACGGGCAAGCATTATCTGCTTCTTCCAATCCGGTTTAGTCACCATACCGAAAT GCCCGATGGCAATCTTCAAGTACGGACATTCTGAAATGATTTCTTCCATCTCGCC CACCTGGAGGTCTCCCTCTGCCATATCTATGGAAAGAATCACCCCCTTGTCTTC CATTAGATGAAACATCCTCATCATCTCGTCCGAGTTGAGCATCACCCTACCGTC CTTCAGTTGCAGGCGGTGTCCCGGAATCTTTATGGCCTTGAACCCTTTGTCTAT AAGTTCAACCGCCTGGTTATAGAAACCCGGTTTTCTGAATTCACACATACCACAC ACGAAGAACCTGTCCGGATATTTCGTCATCACCTCCATCAGATAGTCATTCTGAA TGCCGTCGATATACTCCTGTGTGACAACAGCCGCGCCAATCAGGGCATAATTCA TATTAGCCAGGAAAACCTCAGCCGTGTTTCTTCCGTCAATCATAAAGGGGGGGG GAGCATTTGTCTCACCTCCCCCATAAACAATGATTGACCGTTCTCTGTAGTCTTG ATTTTCAGGCCATCTACTTCAGTGTCCTGATAAAGCCACAGATGCGAATGGGCG TCAATTATTGTATAATCCATAGAAACAGTATTTATGAATTTGCCCAACTTACTCTT TGCTGATCGCCTATTATCTCCTTAACCTTTTCCACAAGGCTCCAGTCTATCGGTT CCTCAATGTATTTTATGTTCTGAAGCACAGACTCTGTTCTTGCCGAGCTGAACAA TGTTGTAGGTATTCTCGGATTGCTTACAGAGAACTGCACCGCAAGTTTCTCGATA GGGTATCCCTGTTCAGCACAATACTTGGCAGCCTTTGCACACACCTCAATCAAT GGTTTTGGAGCCGGATGCCATTCAGGAACACCTCTATGTGTGAGAAGTCCCATA CCGAACGGCGAAGCGTTTATCACTCCCACACCATTTTCGTCAAAATAGTCGAGG AAGTCCACCAGCTTGTCGTCGTTCAATGAATAGTGACAGAAGTTAAGCACCGCC TCTACTGTACCCGGAGCGGCATGGTCGATAATCCATTTCAGGTTTTCGAGCTGC AGGTCGGTGATACCCACGTGGCCCACCACGCCTTTCTTCTTCAGTTCCACCAGA GCAGGCAATGTCTCGTTCACCACCTGGTTCATATCCGAGAACTCAACGTCGTGA ACGTTGATAAGGTCGATATAGTCGATGTTCAGACGTTCCATACTTTCGTAAACAC TCTCCTGAGCGCGTTTGTCCGAGTAGTCCCACGTATTCACACCGTCCTTGCCAT AGCGTCCCACCTTTGTAGAAAGGATGAACGATTCTCTTGGCAATTCCTTCAGAG CCTTACCCAATACGGTTTCGGCTTTATAATGTCCGTAATATGGAGAAACATCAAT AAAGTTCAGTCCGCGTTCCACTGCTGTAAAAACAGACTGTATAGCGTCACTTTCT TTGATAGAATGAAAAACTCCGCCCAATGAAGATGCGCCATAACTCAATACAGGA ACCTTAAGTCCTGTCTTTCCCAATTCACGATATTCCATTTTTGATAAATAATTTAA AGGTTAATATTTTTTACTCTGTTTATTCTTATTCATACAGATAGAACATACGTTCCA TCATCTTCCATTTCTCGTCCGATGTGGCCCCCTCGGCACACTGCTGGAATTTGG CTACGTATTCTTCCCATTCGGCCTGACGCGGCAGAGTGGCAAGCTTTGCCATAG CTGTATCCCAGTCAAAATCCAGAGGTGTTTCCACTATCATAAAGAGTTTTGACCC CAATATGTATATTTCCATTTCCAGGATTCCCACCTCGCGTATTCCGGCGCGTATC TCAGGCCATGCCTCTTCCTTACTGTGAGCCTTTCTGTAGGCTTCAATCAATTCCG GATTCTCACGCAGACTCAATGTCTGACAGTATCTCTTCACAGGCAGGGAATAAC TTTTCACTTTATATCCTTCTGTCTTCATGATATTATTGATATTAATATGTTAGTATT ACATGTCACTGTCTTTATCTTTTCGACGATGCTAAAGTATGAAGTATCCATCAAAA CAATAGAGGAGATTTTCAAAAAAGAAAGAGGGGATATTATACCCCCTCTTTTTCG ACATTTTTACCCCTCATAAAGGAGATAAAAAGTCACCCCAAACTCTATAAAAAAT CAAAACAGATTGAACTGCATTCCTGTGTAGAAAAATCCCTGGTTGGATTTCGGAT TCCAATACGTCATCACCGTCAACGGGATTTCATATTCCATAATCCGAAGTTTATA AATCACATTCAGGGACACCTGAGTAATTCCTGCCGATTCGGCATACATGGTTCT GTTCACCATTTCCCCGCTTTCATTTCTTGAATTTCTCAATGCGAAAGCTGTTCCA ATACCAGGACCGACCCTTAGCTTTTCGTTCTGATAGATGGTATAGCCCACATATA CGAAACTGGAGTAGATGTTCTTGCTGTTGTCCAGATCCCTGTCGCGACCGTAAA CAAGTGTAGAGAAGCTCAACTCCAGCGGAAATTTCCTGTCGCCCGTATAATTGA CCATGAGATCAACGAAACGTCCAGTTTCATCAGGCTTATAGTTGAAGAACTCCTT ATTATTATATGTAGCCCCGGGCGAGAAATTATATGTATCTATAGCCTTTATCTGA AACCTGCCATGAGTATATGCTATATACTGGCTCAGCTCCTTATAACTCCCCCTGG TGTTCGATCCGCCAAGGAAACCGGCGGTAAACCTCCCCGATGGGTCGGAAACC GACAAATCGGACGAGAGAATCAGTCCGTCGGCCACTTCAATGCCACGCCATAG AATCATGTTCTGTAGAGTAGTACTGAAATGAAGCTGAGCCTGAACATTTGCTGAC AAAAATATAAATACAGGAATTAACAGTCGCTTTTTATACTTACAGGTATCCAATGA TAATATATGTATCATACTCAGAGCAGTAGAAAATCGGTTTTAAATTATTATTATGG ATTTATTTGTCGAAATACTCTATAAGATTATAAACATTCCAGTTAATATCCGACAT GTATTTGGTCAATGATGTATAAGGTTTATAGTTATAATCGAGCATACCTTTATTGC AATCCTCATCATCCAGATACTTGAAGAAAACCCATCCTACACAATTCTTGGCTTC GAGCAGTCCCAAGGTAAAATGCTGGTAAGCGAATCCACGGTTTTGCTGGTCGC GTACCACGAAACCAGCTCCACTTGAATTGTCAAGCTTAGTATCCTCACCCTTGGT ATAGAATTCCGTTACCATGAAAGGAGTACCGCCCGCCTGGTTCTTCCAGCCATC CATGTAGCCTTTTTCAGGCGACCATTTACTATAATAATTTATGGAAATGACATCAC AATATTTTCCCGCTGCCTTAATTATATAACTGTTGTATTTAGGAAGGCTGTGCAG GCGTGAACCCAGATAAAGCAATTCAGGATCCTTCGATGCCTTAACCGCATTCTTT ATGGCAGAATAATATTTTTCCGCACAAATACCGGCAAACTCATTGTTCAGTTCAT CCGTTACATCAGAAACATTTGCACTCTTGTCCTTATCCGTCATAAACTTGGCGGC TGCAATATAAGCAGGATCCTGCTTGTTTGAAATTTTCAGGAATCTGTCGAGCAGC CTGTTTCCCCATGTAGAGAAGTCTATCTCATTATCCGAGAAGAATCCCAACACAT CCGGGTTGTTTCTGAACATGCCGAAAGCATCCGAATTGAGATACTCCTTGCACC ATTCATCCCATCCATCATAAAACACAAGACCTATCTTAAGATTCACGTTCTGCCC CGGATAGCTAATTCCCTTGCTATTCTTGAACTCTGCAAGGAATGAAAAGGAAGG AGCCTGTGTCAGAGGACTTGAAGCCGATTTATTATAATCATTTACAGCCTTGTCG CCTTCTTCCTTACCGAAAGCGCAGACACTATGAAATCCTATTTCAGAGAATTGTT TCTGCGACTTTGCCACCCAGTCATCTACTGAACTGTAAAGCTTGCCGAAAGCTG AGCTGTTGCCATCCATTCTGAATGAGGCGATACCCCTTACATAATATGGATAACC TTCGGGGTCGACTATCCAACTTCTTCCATTTGAGTTTTTCTCAACCCTGAACCGT CCAGTAGCCTTGGATTTTTGCCCTTTTGCGTATGAGCCATATTTATTCACGCTTT GCAAATACTCATCCTGTGTTTTTGTCTGCTGTTCATAACCAACCAGGTATGGCAA TATCCTTGTCTTTGCCTCTATAAAAGCCTTGTCAGGTTTTTCCGCATACTCGACA ATTATCGGTTGATACTGCTTGGTGCTATTAGGATAGGTTTCAGCAGGACCGGGA ACAGGCAGTTGCAGTTCTACATCATCATCGTCATTATCGCCTGCATTGTCGCCG GGAGTATTATAGTCCTCCACATTCCCCGGTTGTGAGTAAATAACCTCAGGCGGA ATATATGAGAACTCCTCCTGAGGGTCTTCACATGACAAAGCGAAGAACGGAACA CTCAAGCAAATGGTTTTAGTAATAATAGTAGAATATTTCATTGTTGCAAATATTTA GTAAATTAATATAAATCCCATGTCCTGATTGTATCCCCCCATCGGTGGTCTATCG GGAACTCCATTTCTCCCCATGCCTTAACAGAAGTCCAAGGTTGGTCGGCATCAG TCCAGAATGGGTCAGAGGCAGGCAATCCCAACGGAAGGAATGCAAGTGTAGTC ATATACAGGCTGCCATTGTTTGTATAATGATTCGAAATGCCAGTCTGATGTCCGC AGAATCCTATGGTGAGGAATCCGCCCTCATTGAAGTTATTGCCCGACTTGAACA TACGTTTCATACACGCTGTCAGCGCACATCTCACCTGTGCTTTCGATACTCCCG CCGGCAACTCATTATACCATGCTATAAGAGCCAGTGGCTGCATTGTTGCCATAC GGTAAGGTATAGAGCGTCCGAAAACAGGGAATGTTCCTTCAGGAGATATGAAAC GCTCCAGAATCATGGCGAACCTCTGTGCCCTCATCAATGCCCTGTCATAGTACT TGCGATAGTCGAAACGTGTCCTCACGCCCGATTCCATTATTGCATGTATAGATTC GAGATACATAGGATGGAACACATAACTGCTATAATAATCGAATGCAAAGTGCTGT CCGTCTGCGTACCATCCGTCGCCTACATACCATTCCTCCACCTTGCGGAAAGTA GAATTTATACGATATGTATCCTGTCCGGCATCAATTTTGGCAAGGAAGCTTTCAA TGGTGGCCGAGAACAGCAGCCAGTTAGTGTAAGGAGGGTCAATGCGTCGGAGA CCTTTGAACTCTTTTATGTAGCGTTCCTTTGTTGTCTGGTCCAGCGGTTTCCACA GCTGGTCGAACGCGCGCAGGAAACTTTCCGCAATATAGGCAGCATCAACCAGT GCCTGACCATGACCGTTCCACAACAGATAATCCGGACTATTAGGGTCCACCGCA TTTGCATAACTCTTCAATGCCCATTCTTTCAGTTGCTTGCGCTGCTGTCCTTCTG CTGTATCATCGTCAGGCAGGCTCAACCATGGAGCTATACCGGCCATGAGACGT CCGAAAGTTTCCATATATGCAACCTTCTTGTTACGGTTATCCCAGTTTGGACTTA CCTCAAGAATCATATTTTTCTGCAGTTCCCCTTTCGCCATATTGCTCAACACAGG AGCAGCCATCCTGTAAGCCATATCCGTCCAGTATTTTCTTGTCTCGTTGTTGTTT GCCTCGAGATAACGCACATACTCGCAAGCGGCAAGAAGGAATGCGCCTACCCC AAAGTTGGCAGTCGACTTGGCGTCAACCACCTGTCCCGGAATAGCCTTTTCACC GATTGGCTGGACATAACCCACCGACCAGTCTTTCTGCAGTGCAGTCTTGGTAAG ATATTTCCATGCTTTCCCCACTACAGGCATAAATTCATCCTTGTCAAGATAACCG TTGTTTATCCCCCAAAGCATACCGTAAGTGAAGAAAGCGGTACCGCTTGTTTCC GGTCCCGGAGCATGTTCCGGATCCATCATACTTCTTGTCCAGTAGCCCTCCGGC TGCTGCAGACATGCAACCGCCTTTGCCATACGCACAAACTTATCCTCGAAAAAA GACAGATGCTCATAACCCTCCGGCAGGTCCTTCAGCACCTTTGCCAGAGCGGC AAGCACCCATCCGTCGCCTCTTGCCCAGAAATCCTTCTTTCCGTTCAGACTCTTA TGCTTGGGATAAACATATTTTGCGTCGCGATAATAGAGTCCTTCCTCCTCATCAT ACATTATTGAGTCCGACGTACAAAGATATTCATACAGTTTCTTAAGATACCGGTG ATTATGCGTAATCTTATACATCTTCGTCATTACCGGCATCACCATATAAAGTCCG TCGCTCCACCACCAGTAATCCTTACGCGGTGTGCTCATCTGGTACTCCATGACT TCGCGTGCACGCTTGATTTTATAATTCTCCGGCATGACGTTATACAAGTCCGCAT AAGTCTGGAAGCACACCTGATAATCGCCGAACAGCACATAATCATCCTTTACCC CGTATTTATACTTCCATTCAGATTTGTTGTTGCTTTTCGCACCCATCCACTGGTTA TACTCAGCCCATGCCTCCGAATACTTTCTGTATTCTTCTTTCCCAGTAAGGAAAT AGGCTTCCATATTACCGGTGTGATATGCCGCATAATCCCAGAAAGACCTTGCTT CGGGGGCATGATTTTTCTGCCAGGCATCGTTCACTTTTTCAATCATCTCCCTAAC TTGCTGAGCCTCAGTTTTTTTTTGCGAAGGAAAATGAAGGTAAAACAGCTATAAG GATGTATAACATCCAGTAGTATCTATAACAGTTCATCTTTGTGATATTGTTTACAT TTTCTAAAACGAAATGGGGAAGAATATATATTCCTCCCTCATTTCACGAATAATTG TATTATTATATTTATTTGTTAGGAGTCCATTCTGCTCCGTTGTTGAAACCTTCTGT TGTAGAGTCAAAACTTGCATCTGCTCCTGTACTTGGTCTTTCTGTAATTTCTTCAA TCTTAAAAGAAGTGATTTTAGCGGTTCCAGTAGCATCAGTACCACCAGGGACATT AGTCTGTACAGTTAAAATAACGTTCTCAAGAACCGGCCACACAAGTGAACCATCT GCTCTTGAAGCTGGAGTTTCAGCAGAAGTAGAACTACTGATTGTGAATGTATTTG TATAGGTTCCACTTCCGGTATTTCTTCCAATCCAGAATTTATATTTATCAGATGCT CCCAATCTGAATGTTGTTGCACAGTCGTTAGATGCGTATGTATAAGTAAATTTGT AAGTACAACCATCACGGAATGACATTGATTTAGTAACTGGGAATTGATTATCTGC TGGAACAATTTCCAATTCTCCACTTGCATTAATTTTTTCGGCAACTCCTTCTGCAA GATATTCCTTAACTGCATCTATGTTAGCGAAGTTAAAATCAAAAGCATCTGCATG AGTCAAAGCAACATTGGCAGATTCAATCTTGATATTAGCATCTTCGTTGTTTTCG TTTTTAGCAGTCAAAGCACTAACAGCATAATCAGTGTTATAACTTACGCTAATATT TGCGTCATTACTATAAATCTTATCACCAAGAATAAGAGTCATAGTAGTTCCATTCA CAGAACCGGAAGCAACAGGAATTGTTTTTCCTGCTACTGTTATGGTAAATGCTTT GTTAACAGCATCAGTGAATGTTCCAGAAACTTCCTTATCGAGTGTAAGTTCAATT CGGTCATTACCTGTTGTCTGATCAGGAACAATTTCTTTAGCTGAAGAAACGGCAA CAGTAGTTTGTTTTTCCAAATCCACAGGAGGTTCACCGCCTCCTTGATCATCATT CAATACTATCGTTACAATCTGTCCTTTAGTAACTATAAGGTTTTCACCACTGAAGT TATAAGTTTTAGTACCAGAATTTCTTGTAAGTTCTAAAGTAAATCCATCGGTAAAT GTCACCGGAGCTACAACCATTGAGTATTCCTTGGCATTTTTATTTTGTTCATTAG GACCAACAAATGTTCCCTCTTTAGCGGTTAGAGTTATAACATTAGAACCGGATTC CACTGTCAGGTTTGCTGAAGCATCAATTTTTACGTTCCCTGCAATCTTTACATCA CCACCAGCAGTAAGTTTAATACCTGTAAGGTCAGTAAGATTATTTTTAAACTTAAC CAATCCACAAGTATTCTGGAAAGTTAAAGATTTGTTATTATCTGTTGCAGTAGCAT AAGATATATTTGCATTTGCATCGAATCCCCAAGCCGGAGCTGTCTGTTCAGATG GCAGTGTAGTAGTTACGACACCTTCAAGACACACAGCTTCGGCATTATAAGGAT AAAGAGCTGTATATGAATTGTTAGGTGTAGCCTTACCTGTAAACGTTGTAACTGT GCTACCACCTGTAGCGGTAGTAAACTTGTTATTTTCTTGGCCTGAAAAGATATTG ATTGCATCTCCTGTTGTCCACCACACCGTTGTTCCATTCTGCAACGAACTACGG CTTGAAGGCGTACCGGCAACAAAAGTCATATCCTGAGGACCACTGACTGCATTT ACATTCGACAGTTCGTCTTTTGTACAAGACTGGAGCATTGCAATACTCATCAAAG CCGCTCCACAAAATAGCATCGTATTTTTCATGACATAAATTATTTGTTAAACAGTT TCAATAATAAAAAATCACATCACTTGTTATTCATATTCTTATTCTTTAGGATCAGGT TTCCATTCAGTACCGTCATCTTCAAAATCATCATGACCGCCATCTACAATTCCGG GAGGTATTGATATTCGGCATACCGCACTTTTTATTCCATTACCCGTATCTACAGA AGCACCGATATTAGAATCTCTGCCCCCGTCGATTGCCACGACCGTACATCTCAT TTTATCGTCCGATGGTGTAATCATCAACACATCAGGGAAAGAAGTTCCCCAAACT ATTGACTTGTAACCGGTATAAGGGAGATTATCCTTGGTTATATTAATACCCAACT CCACAGTGCCACTATATGGTAATTCTATATAACTGACAGGTTTGTTGTCAGTCTG CCCATCCTTGAACACTACATATTCAATTTTTATCTCCTCAGCTGGTGTTCCATCAC CTCCACCTACGCCATCATCATCCTTATCACACGAGATTGCCGTAAACTGTATAAA AAGAAGTATGAAAAGGTTGTATACTGACAGAATCCGTGGTTTTATATCAACCATA ATAAAATGTTATTTAAGCGCCAAACAAAATTTTCAATATTCAAAAGGCATAAGAGG AAACCCTGAATATGCCTTATTACCATGAAAACAAATCAATCTACCTTTTTCAATCC GGAATCAGAAAAATATGTTATTTATTTAGAACATATTTTTCCGATTTGCCAGATTA CAATCACAATAAATAAATCAACAACTAAATCTAATTACCTAATCTTATAACTAAAC CCTCAAACAATGTTATTTAACCTTTTCTATCTTGACATCATCAAGCAGGAAGCATC CACCATTACCTGAACCCGGAACAGCTGTGAAACGATATACAAAACCATTTTCCTG CAATTTGAATTTAACTGTTGTAAGATTGTAATTCTTACGGTCTTTCTTGACCTCAG CAGTGGCAATTTCTTCCAGTTTCTTTGAATCCGGATTATAGTACTCAATCCTGAA GTTAGGTTTGTCACCCCAACTGTATTTGGTATAAGCTGAAATCTGATATTCTGCT CCAGTTTCATAGCTGATGTTTACAGCCTGCCACATACCAACCTTCACCTCAACAG CATAGTTGCCTGAATGTGCCTTTTTCGCATCAACTATTTTGTTATCTTTCTTTTCC CAGACATTCCATGATGTCAAGTCACCTGACTCAAAATCACCGTTCTTAATTTCCT GAGCGTATGCAGAAGTCATCATCATTCCGCAAGCCATCATTGCTAAAATTTCTTT TTTCATTTTTTCTAAGGTTTTTAATTTAAGTATTATGTTGTATCTATTAAAATCACT CTTCTATTGGAACCAACTTATAAGCCCTGACCCAGTCATAATAAGTAGTACTTTT GTCCTTATCCTTCAAGTCCTCAGCTGTAGGTACTTGTTTTTCCCAATCGTATGTTT CAGTAACTATATGTATGAACATAGGTCGGTCAAACGGAGTATCTGTATATTTTGT TGTAGGCTTGATAGTGTACATATACTTTCCGTCATAATAGAATTTCACGGTATTTG CATCCACCCACCAACAACCGTAAGTATGGAAATCTTCTGCCGATGGGTCCGTCA TATACGAAACCACATCCGAACGTTTCGCCGTATTGTCAGTACGTTTGCCTCCTTG TTCCTGATACCAATAGTGAGTATTACTGTTCATCTGCATATTCCATGTCTTGTTCC ACGGATTATCAGGGTTGACACTTCTTATTATACCCATTGTTTCTATAATATCAAGT TCCTGACTGCTCCATGTCTTTATCTTCTTGCCGCCTTTCATTATTTCCTTCATTAC CGGGCGGTTGGAAAGCCAAAAAGTAGACGACATGGTAGTGAGCGAAGCCTTCA TCCTTGTTTCATAATACCCATAATGTGCCTGGTTCTTTGCAGAAGCAACCGCTCC ACCGGCAAGACGATATTTATCGCCCGGCTTTCCATCAAGTCCTTCTGTTGGCGA CAAAACGGTATTGATTATACGAAGACAACCTTTCTTGACACTAACATTCTCTGCC TTGAAAGTTGCAGGCGGCCGACCGTTAGTCCAATAAGGACTTTTAGCATGCCAT TTAGCGGCATTAAGACGTTTACCATTGAATTCATCAGTATAATCTTCGTTAACTAC CCATTTATAACCCTCAGGAGCCTCAGGCAAATTTTTTATATGCTCTTCAGCCAAA GAATATTCCTTATCATTTTTTAATGTATAAGATGACAGGAATAAAGATGCAGCAGA TAAATACAATACTGTTTTTCTCATAAACTTTGTCGTTTTAGATTTTTTGTTACACGA CAAAAGTATATAAGTTTCATGAAAGCATTAAGGGGGATTTACATCGTAAAAGGTG GGGTAAAATTCTACCACTCCCTGAAACACAATTATTTCACTCATGAAACCATGTG TTTTTACGATATATAAAACCCGACAGAAGAATAATACCGTATTACCGGCTAATTTA CATAAGAATAACTTTTCAAACCGCCATATACCCCACTTTACGTCCGTACCCTCAG TCCTCGACTCCGGCAATATGTTTTCCATATCGAGATCTATGGTTTTCTGCCTCGG ATTCAACCACTAACTGTCGAGCATGTGGATTGCGTATCTGTCATAGAATCTCTTT CCGAACCATATTATCTCGTCTGTGCTAAGTATGTTGTTCAGACGGATAATCTTTC CGGTATTTTACCACCTACTTCTCTTGCAAATCCTGATCTGATATAACCGGATACT CTCAATTCATTGATTTCCGACTTGTATACAGTCTGCGAAGAGGCATTGAAACTAC TGCACAGACTGAACAGCAGCAGGGGAATAATTTAACTGATTTTAATAGTAGACAT TCTGTGTTCATAATATTTCATTTTAATGATTACGTTTCTGACTTTCGTCTGATGCA AAATTATGAGGTATCGGACGGGGTTGTATCTTTCAGTAAAAATCAGTAAAGTCTT GGCAAGGGGTAAAAAACTTAACATCTTGTATATAAATATATTACAAACAAGGTGC AAAGATTTTCAGTAAACGATGGCGAATACAGAACCTATATATTTACACGCCATAA AATGAAGAAAAAGCAGTAGGAAAAAAATGCGGGCAAGTTCCGGATAAAATGTGG GCAAGTTTAAGGTAAAACTTGCCCGCATTTTAGATAGAATGCGATCGCATTTAAA ACAAGTAAAAAACGAAGAAAAAAAATATGTGTTCTTCACAGAACACATATTTCAAA AATAGGTATAAACACGCTAAACAATGTTAACAAAATCTATTTATAAAAAAAGCTCA CATCAATAATATCTGCAACATTTTTACAATACTCCATAAATGAAGAGACCTTGGG ATGATTTATACACAGAGCTATCTGTGATGTAGGCGAAAAACGTCCTGTCCCGTC AAGAAACGCTGTAAGCTCAGATGGGAGGAGTATACTGCCAATACCTGGATTTAC GTCAGTCAGAACGACTGTATTTACAGCTTCCACCGCTGACACATCAAGATAATC GAGTGCCGGAAGATCTGCGAAGTGCAATTTTCCTATCATATTGCCGCCTTTGCT GCCCTGAAGAGAGACACTCTCCAATGAAGAACAACCGGATATATGGATTTCACT GTCGAATATTGAAGTTTCGGAAACATCATCATTAAGTATAACAGAAGGAACAACT ACCAATTGAAGCGAACTGTTATTCTCCACCCTAAGTACTTTCAATGATGATGCGG AACTTAAATCCATTCCCAAAGGTGTATCAATATTAGAGATTGAAAATACTGAAACT CCCGAAGACGGCTTGACATACGACATGGAATAATGCTTGGACTTCACTCCCGAA ATGTCTACTTTTCCTCTGAAACCGGGATTTGACAATATATACTCCACACCCTCAA GGTTAGCTGTCTGCGACAGGAAAATGAGGTCGTTCCCTTCGGTTATCCTCTTCG TGACATCAATCTCCAACGATGAGACAAACACCGACGGGAAGTTTCTGTAAAGAT ATGAACGGAGCAAAGGATCCGGTACTCTTCGGTTTACTGTATATTCAGTGTAATT TCCATCCTCGTCCGACATCACGACAAGACATTTGTCCGTCATGGCTTTATAGAAT GCAGGTATGACATCTGTATTCCATTTTGCAAAGTAAGGAAGTTTCAGATTTGTAA GACTTTTACAAAGCACCGTAGTGCCATCGGTTGAAATAAGATTGAGATACGAAG TTATGCCGTCATTGCCGCGTAAAGCTACCGATTTTATTCCTTCGGGCAGGTCAG CAAAGTCGAATATAGAAAAACTGTTACACTCAAGATTGACATCTGCGAGCGAAG GAAAACTCCTCAAACCGCTAATAGATGTAAGTTCGCATCTACTCAAGTCCAAAGA AGTGGTATTGAGAACTTGATTGTCACAAATCAGCTCTCCGTTTTCGCTGAAATTA AATCCTTTCCGGGTCAAGACATCGCGTAACTTTGTATCAAAAGTCACTTCAGACA CTTCAAAGTCGGAAATTTCTGTTTCATCCTTACACGAGATTATTGTGAAACAGAG AACTATCAGTACATAAAAGCTAATAAAATTCCTCATAACAATCAGTTTTGTGGTAA TAAGACTATATTATCAATCCAAGCCGCGTCGTTCTGTCTTTCGCACACAATGGCA CACACTACTTTTTTCACTGTAGAATTAAAATCGAAAGATACGGCTTTATAATTGCC GGGAGAAGAAAATTCCTCTGTATATACCGTTCCTGTAGACATATCCTGTAGCATG ACTTTCAACTTACATGCTCCTTCGGTCTTTACATCAGCAGAGAAGCGATAAGTCC TGCCACTCTCCATGTCAACCCTCTGCATGAGTCCTGCATGACCAGATATACAGG CTACATTATTGCCTGCATTGTCAGTCTGTACGCAAACCGTACCATAGTTACCCAA TGGCTGCCATGCTGAAAGTCCTTCGCTGAAGGTTCCATTCTGCAAGGTAGAGAC AGTATATTTCTCAACCTGCAGTATCATGGACGATACGTGACCTCCTCCGTCGGA AAAGGTGATGTCGACATTATTATCGCCATTCTTCAGCAGCTGTATGTCGAACGGT ACTTCTATCATACCGAAAAATATATTGCGGTTGCTCTGGCCGTAGCCTTTCCAGT TGTCGGGAACACTCACAGCGGTACCATTAATCTTTACCACCGGTTTCTTGGAAG CAGAGACAGGACGGCCTATCGACATACGCAAGCTTGCTCTGCCCGAACCGGAC TCGATTCCTGTGAAGGGGAACGAAAGGGATGATCCGGCGGAAATCGGTTTCAG ATACTCACTGCTGTAATATTTATTGCGGATTATGGAGTTCGTGAATGCTGACGAA GACACATCTGCTACAAGGACTATGGTCTGATTTGGGACAATTGAGATGCTTTCA GGCATGGACGGGACATTCTGTTCCGTATATTCTATACCTGCGTTATAATTGACAT ATAGAGAACGCTTTGTGACATTCGATACATCCTTCCAGCTATTCTTATTGTTCAG ATATACAGTCTGCGGGTTATCATCAAGATTATCAAGGGCGATATAGAGTCTGCCT CCATCCTTGAATGCCTGTACCTGAATATCAGGATTACTGCTGGTTATATCAACAC GTTCGCCTTTTACATTCTTCCAGAGTTCGAAGAAATATTTTTTGTCATTAAGCCTC CATGTGGTATTCTTCAGATTCTGAGGATTGTCGGGAATAAACAGTGCCGCACTA TATGAAGTATAATTGTTTGCAGCGGTGATATGCCACTCAGCCTTATCTGAGACAA AAGGTATTGAGATAAACAAATTGTCCTGACGTTCCATCAGATTAAACAGAAAATG ATTAAACGACGAAACACTCCGCACACTGCTTATGTCATCATAGCTGTCGTCGGG CTTGCTGTTGTCAATACCTCCAAACTCGGAAATGGCAAGAGGCTTGACATGTCC GAACTTAATATAGGAATACGCCTCAACCATATCAAGAACTGCTTCGGAGTTACTT CCTGAACGTTTCGTATCGGTGCCGGTTACATTTATTCCATCATAAAGATGTACAG AGAATCCATCCATATATGCACCTGCCCGATCGATGAACATTTTCATGCGGGTGTT CCAGTAATTGAAGTTCCCATCCTCCCAGGCGGGGTAGGCTGCGGCATAGCCTA TCACCTTCATCTTTCCGTTAAGACGCGGATTATTGTGTATATGTTTACCTATTGAA GCATAAAAATCGACCATCAGTTCGCGCATAGCCTGTCCCTGAACGGTAAAACCG GCATCATTTGCATGAACGAACGGTTCATTGAGGGGTTCAAAAAACTCAGGTACC AGCTCGCTGTTGGAATAATACTCAGCCGACCATGCACCTGCAGCCTGAACGTCT ATGCCGCCCTGTATGTGCTGTACATAGGGATGCTCTGTGGCAATATATCTTTTTA CGGAAATATTTCCGCTGTATGGTTTCATCTGAGGATATTTGCCTACCTCATGCGT CTTGTTATACGCATACGAGTATGGTCCCCAGAACTTTCTTCCAAGACCGACCTG ATAGTCGGCAAGAAACTTGCCTACATCCTTATCATCATCGGAGGTGGAATGAAT ATTGAAATATTTAGAACGGTCGAGTTCTGAAACACCGCTCAAAAAGCGACGGGT ATTATAGTCGACAACCACCTCGTTCCTTTCCTGACAATAAATACCGGGAGGAACA CCTAGGGTAAATGCCGATAACAGAAAAATATATTTATAGCTCATAATTTCTTTCCT TTTAGACACAGAAACTTGTCAGTCCTGATGTGGATACATTATTTTCTCACTTTCTT ATCGTAGCGTTCAGTCTGAAGAATCATAGTAGCCACACGGCCTCCATTATCCGG GAATGTTACTGACACCGAATTTTTTCCTTTTCTGATTAACCGGTAGTCGAAAGGT ATTTCTATCATACCGAAGAAATCGTCTCTGCCGGTCTGGTCATATCCTCTCCAAT TGTCGGGCATGTCGACTTTCTTGCCATTAACCATTATTTCAGGTTTCTTCGACAT CTCGTGCTTCCTGCCTATTGACATACGCAGAACAGCTCTTCCTGTACCCGGTTT CAGACCATCGAAATCAAACACAATTGGTTTTCCGGCTTCCACCGGCTGAAGATA AGTGTTGCTATAATATTTAGTACGAACTATTCTGTTTGAATACTTTTTACGGATGA TGTCGGCACACAATATTATTGTCTCATCTTTTATAATGTCAATACTTTGAGGCATC GAGTTCAGCGTCTTTTCATCATAAACTATACCTTTATCGAAAATCATCTTCAAAGA GCGCACAGAAACATTATCTACACCCTTCCAATTCAGTACGTTTTTCAAGTTTACC TTATGTGTATAGTCATCAAGATTGTCGACAGCTATGTAAAGCCTGTCATCGTCCT TAAAAGCTGCCACCTGTATGTCCGGATTGTCGGAAACAATATCTACACGTTCGC CTTTCACATCCTTCCATAACTTGAAGAAATATTTCTTGTCGTTCAGTTTCCATGCG GTATTCTTCAAGTCGTGAGGATTGTTGGCAACAAATAAAGCAGCTCCGTATGGTT CGAAATTATATTGTTTCGTTATATGCCATTCGGCCTTGTCAGAAACAAAGGGTAT TGAGATGAGCATCTTGTCTTCGCGTTCAAGAAGATTGAACAGTATATGATTGAAC GAAGCGACAGTTCGTACAGAGGCTATCGGATTATATCCTTTGGAAGTGTTGTCT ATTCCTCCATATTCGGTTACGGCAAGAGGAAGAACTTTCCCCAAGCGGATGAAC GAGTAGTTTTCCATAAGGTCGAGAATAGCTTCGGAATTACTTCCCGAACGGCGG GAACTCTTGCCTACTATGTTTATTCCATCGTAAAGATGTACCGACAAGCCATCCA TGTACTCCCCGGCACGGTCAATGAACATCTTCATAGTATTATTCCAATGGTCGAA ATCGCGCAACTCCATAGCCGGATATGCCGCGGCATATCCAATGATTTTCATTTTT TTCAGACTTGGCTCAGCGTGAATATGCTTTCCTGTCTGTGCATAAAAATCTGCCA TGAGCATCCTCATTTCCTGACCATGCATATTGAAACATTTGTCGCGTGCATGGAC AAAGGGTTCGTTAATGGGTTCGAAAAATTCAGGAACTGCCCCTTTCACATGCTT GGAATAGTATTCGGCAGCCCATGCACCCGCCTTCACTGGGTCTATGCCCCATTG TATGGTACGCGCGTTGGCATGTTCCGTAGCGACATATCGTTTTGTTTCCTTCAAA TCAGTGTAGTTCAAAGGCTTTTCTGAAAAAGGATATTCGCCAACCTTTTTTGTCTT GCCATATGAATAAGAGAACGGTCCCCAGAAAGAGCGGCCGATTCCTACACCGT AATCTGCAAGAAATTTCCTGACATCTGGATCAGAATCTTTAGATGTGTGTATATT GAAATATTTACCTCTGTCAAGTGCCGATACATCATTCAGGTATCTCTGAGTGGCA TAATCCACTGTGACAGTAGTGTTATAAGTCTTATTCTCGGAAGATGATAAAGGAA AAACCGAGAAAGACAAACACACAGACAAAGCTGTAAGAATTATGTTATTCATTGT ATTATCAAAATTTAAAAGGCAGAGAACACTCCGATAGTTCAATTAAAGTATTCCCT GCCATTAAGATTATCACTTCTGTTTAAACACTAATATCAGAAATCGGCCGGTTTG AGTACATCGTTCAGCACCACTTCATATTCAACTTCTGTTCCGTCGTTTTCAGTAA CAGTAAGATGGCCGTAACCGCCACTTGAGTTATTTTCTTTCTTACCTTCAAACAT GAACATTCTCTTCTTCGTCACTTCCTGTTCTTCTTTATCGCCTGTTTCAGGATTGA TAACTTCTTCCTTTTCAGTATAGACTTCATTGAAAGAGAATGAGAGATGTTTTTCT GTATCCGAATTGATTTTCAGCCACTCGGGCAATTCCGAAGGAGCCTCAGACGAC TCGGCAAAGAACTCGATCTTATTCATTCTCAAAGTCTGATAGTCATTCTTCCAGG CAATGAGGTCGAACAATTCGCGATAAACAGAAAACTTGGAAACCTCGCCTGTCT TTCCTGTTTCCACATTCTTGAGATAGGTAAGTTCATACACGGGAGTAGAATCAAG TTCGACCTCAGCCCATTTGTCATTATCACATGCTCCGAACAAAACCAAAGCACAT AAGAATGTAATTGTCTTATAAATTTTATCTATTAGCTTCATTGTTACTATAATTTAT TATGGTCTTACTTCAATATATCCGAAAAATATATCGTCAAAATAAATATTATCCTTA AAGGCATTAAAGCGCATACTGAGCAATATATTGTCCATTTCAGCCTTTGAAGTCA CAGTGGTTGTGGCCGACATCCATTTGCTGTCGGAGCCATTCACAATGCCGCACC ATGGTCTATCGCTCTGCCATGTCATATCTTCAGCTCCTTCTTTACCTGCCGGAAC GAAATACGGACTCATACCCTTACCCTGTTTATACCCCGGTGTATAATATTTGTAG CTGAAAGTATATGTACCTTTACCACCAGTAAATGTCTTGGAGAGTAATGCCCTGC ATCGGTCAAATGCTTCGACAAACATACATTTTGCACTGTTGTTTATTCCATCCTTC AGAGGATTGTCCACAACCTGTGAAGGAACTACAGGATGTGTTTTGGTATCGGCA TCAATAACTTTCCAGTCGGCATATGTGTCAGAATTTTCAAAATCTTCATCCAGGA ACGCACCAAAAGTAGTCGCTACGTTTGGAGCTGTAGCCTTTATCTCAAGGTTCT GATATCCAACCAACGCTTCAGTTAAAGTTCCTGTAAGGGTCAGTTCATCTGTGTT ATAGATTTTCTCAACCAAAGTAAGAATCAGTTCATATCTGCTTTGCTTGTTTACTT CTGCTGCTGTGATGTTTACGCTACCCCTGACAGCTGACGGTCTGTTATACGAGT TGGAGTAAGTAAGCTTTAGAGATGATGGATTTATCTCTTTATATCCAAACTCAGA ATTATCCAAATCTATAGCAATGTGTGTTTGGTCAATCTGACGGATGTTATAAGTA ATAGGATCATCACTAGGTACTACTGTAATAGCCAAAGGCACAACAAGAGTTTTTG GCGAAGCTTTTGGAGTGTACTTACCTTTACCCTCACTGGCAGAAGTTCTTTCTAT TGTCATGGAAAGAAGCAATGGCTTATCGCTGAATTTCTTTGCAGTGAACTGGTAT GGAGTGTCAAAACTGGTTAATTCGTCATTTACGCCAGTATCCGCACATTTGAAAG TCCATTTGTTAGGCAATCCGTATGAATCGTCCTTAATATAGACAGACTTACCATA TTCAAGTTCGTATTTTTCGTATTCGGGAGCTTCCTCAGTTCCACCGACTATTCCG GTCTTTATTTCCTGTGTACACTCCGGATCACTGTATACCTTTACGGCCGGTACGA GGTTAGGATCATACACGCGGATATGGAAAGTTGTATCCATCACATATACATCACC CTCCTGCTTAGCATAACAATATTTTTTGATATATCCTCCGGTATTGTCGTCATACA CCGAATATGGATATACAACCTGTCTGCGGAAAGTATTGCACAAACGTACCGTAT GGTCACCGGGTTTAGTGAAATACACATGTATGGTTTTCAAATCGTTGGTATGAG GGATGGATTCATCAATCAGGTTTGTATAGTCTGTCTGTCCCCACTCCATCTTACC ATTAAGGAACTTTGTACCATCATCCGACACAACCCACTGATGCGACAACATGCC TTGGGATAAGTCCATTATACTTATATAGTTATTAAGATTCAGCTGAATAGGTGAAA CGTTTTCCTGATCTGTACTCACATGCCAGGTACATTCAGCCACGTTATTCAACGG TTCAAACTCATCATCCTTACAAGATGTCAGAACCGAGATTAATGAAAGAGCAATA TATAAAAATCTATTTTTCATCGTATTTATTTATTAATATCAGGATTTGATGTAATTT CTATATTTGGAATAGGCCAGTATGCCACTTGCGGACCGTAGTTCAATGATGCTT GGAAATAATCCACAAAAGCGTTTCCTCTCTTTTCTGGCGGCAGCTCATAAAATCT GTACTGCTTTCCAAAGTTGAATGCTGATACCAAAGCATTAGGGTCATCAGGATTA GGCTTAAGATATTTGGTCTGAATCATACAGTACTTATATTCGTCGGATGCCAACT GATCAAACCTTTCCTTAGTTATATTCCAGCGTCTCAAATCAATGACACGTATGGC ATGTCCTTCCATACACAGTTCAAGAGGACGTTCCACATACATCAGATGATTCATT ACATCACTTGCAGCATATTCCTTCTCATCGTATGTATATCTCTTGAATTCTCCCTG TTCCGATTTTCCGATAAGCACAACTCCAGCACGGTGACGTACCTTGTTGATGGC ATTGATAGCTGACTGAACATTTCCATCGCTTGCACCGCCTTTAATCAGACATTCT GCATACATCAGATATATATCTGCCAAACGGATAAGACGATAGTTTATTCCTGAGG CCATAGCAGGCTTAAATTCAGTTTCACTCTTACGTGTATCCCAATTTGATAATTTT CTGAAATACGCTGAAGAGCCACGGTTGAATTTTGATACCTGTTGTGGGAGAGAC TGATAATATATCAGACTTTCATCGCCGTTTATTGCAAGAGAGGCAGATGCACGCA TGGAATAGCTTCTGAGGCGATATGCCTGACCGTCTTCCCATTTAAATTCCGGAA CTATGTCATCGTAGCCGGTAATCTTATTGTATAAAACTTTATTATCTCCGACAGTT GAGACGAGTCGTTCGCGTACTCCAACATATTTTCCTGCTGTTGCATCCCACGTA TAAACGTACGTTCTGTTATATACGACACCCTGACGGTCCACCTGCGAGCTGAAA GTTGTTCCCAACTGGTCGTATATAATATCCCTATGTTCAGGATCACCATAATTGT CGGACTGCATTTTTATCCAGTTACGTTCATCAAGTCTGTCCACCGGCTCTGTTTC GAATGCTTCAACAAGCCAAAAAGCAGGAACAGTGTTAAGCCAGGCATCGCCCAA GCCATTTACATTCATTCCCCATATATTATATAAGGTAGACTCCGACCATGTACCG AATTCTGTATTATACTGTGTAGAATAGGAAACCTCGAGAATAGATTCCGAATTGA ATTCATTGGCAGCAGTAAAATTATCGACTATGTCATCAACCAAAGCAAAACCTCC ATTATCAATAATATCCTTAAAATATTCGGCAGCTTTATTATACTCTTTATCATAAAG GTAGCTTTTGCCTAATATTGCCTTTACAGCCCAAGAGGTGATACGTCCCAAATCG GTTTTCTCCCATTTGTCATTCAAGCCAAGGTCAAGAGCTTTCTGTAAATCTTCTCT GTAATATTTCTTGATTTCATCACTTGGTGTAACCTTTTTATAGTAATCTTCTTCTAC CTCTGCAATTTCATTAATATAAGGAACATTACCATTATTGAATGAATTATTGAGAT AAAAATAAAACAAGCCACGCAAAGAATATGCCTGTGCCTCAATCTGAGCAAGCT TGGTTATTTGAGGTTCATCTGTAACATTTGGACGGATTTTCTCTATACTGGCCAG AACCTGATTCGCACGGAACACACCAGTATACAGTGCAGACCATTTACCACGGAC TGTTCCGTATGAATCATTAAAGGTTTGCTTATAGGCTTCGTTATCAAACTGCTTTC TGTCCTTATTACCTTCAACTGCTATATCACTTCTACGGTTCTCATCGAGCGGATG ATAAATATTGGTATTTTTCAAAGCATTATATACAGCAGCCAGTCCTTTCTCGCAGT CGCCTATTGTTTTATAAAAATTCTGTGTTGTCAGCTGATGTATGTTTTCCTGCGTA AGGAAATCGTCGCATGAAACCAATGTCATGCCCGACATCAACAGACTGAATACT ATTGTTTTATATCTGAAGTTCATATATTTATATTATTAAAAGTTAGAAATTAATCTG GAATCCGCCACGCATCTGGATACTTATAGGATATGTTCCATAGTCCAAACCACG ACGTGACAATCCATTACTACCGACCTCAGGGTCGTATCCGTCGTATTTTGTCAGT GTAAGAAGATTATCGGCTGCAACGTATAAACGGAACTTGCCCAATCCAAGCTTT GATACCCAACTCTTGGGGAATGAATATCCTAACATAATATTTTTAAGTCTGACAA ATGAACCGTCCTCAATCCACATATCAGTATGAGCACGATAGTTGTTATGCCCCTC TGTACGATAAGAAGGAATGGTAGAGGTATAGTTGGTAGGGGTCCACATGTATAT CAGTTCCTTATTGGTTCTTCTTTGATATGTATATATCTTCGTACCGTTTATTATTTC ATTTCCAACTGAAGCATACCAGTTCATAGAGAAATCGAAGCCTCTATAGTCGGC CGAGAAGTTCAAACCAAGTTCATAATCCGGCATACCACTACCGGCATAAACACG GTCGTCATCATTAAGAACACCATCATTATTGGTATCGATATACATAAGGTCACCC ATACGGGCACTTGACTGTAATTTCTGATATTCTGCAAGCTTCTGTTCAGTATTGA TTACCCCTGCGGTTGGCATAACAAAGAAAGCACCGGCTTCATATCCTTTCTTGAT TGCAGTTACATAATCACTTCCTGATGAAACAGGTTTACCGTCGGGGAAGAAATAT AACTCATTTTTTCCTGCCATAGACACAATCTCATTCACGTTTTTGGTAAATGTACC AGTCAAGCTGTAATTAACACCACGTATTTTGTTGCGGTGAGTAAGTGAAAACTCA ACACCACGGTTTTCCATATCTCCGGCATTCAATGTAACAGTTGAACTCTGGCCC CCTCCATTTGACGGTGGCACGACCATCGGGAAAAGCATATTCTTCTTGTTACTCT TGTACAAATCAAGACCTAAGATAAGCTTGTTATTATATAAAGCCATGTCGATACC GGCATTAAGCTGCTGGGTTGTTTCCCATTTCACATTCGGATTGGCAAATCCCAAT TGGGTAAAACCATTTGCAAGAATTTCGGAAGTTCCGGTACCAAAAGTATAGTCGT AGTTTTTGTATATAGCTGGTGCGTATGAATAATCAGGGAAGTTCTGATTACCGGT AGTACCATAGCTGAATCTTAATTTTAACGAATTTACTAGCCACCTGAATCTGTCG AAGAATGATTCCTCAGAAATATTCCATCCTACAGACAATGACGGGAACAATCCCC AACGATTTTCTTCGGAGAACTTAGATGAACCGTCGCGCCTGATACTGGCACTTG CCATGTATTTGTCTGCATAGCTATATTGTAGACGACCCAACATACCAACCATTGT ACTGATACGGTCCTGTCCCCACTGGCCACTGCCTGTACCCACAGTCATATCGGA TGTTCCCGCATTTAGGTTCGGAATCTCGTTAGTAACCAAATCCATTATACTGGCA TAGAACATCTCGTATGTATATTTCTCCATACTGAAAACTCCGGTAAATTTAATATC ATGCTTTTTTATCTTCTTATTATAATTTACCATTGTTTCCCAAGTGAGACTGGTATT CTTTGAATGAGTATCTTTTAATTGCGAACGGTAATTAGAGCTGGTTACCTTTTCG CCTTTCTGATTATATACCTCAAACTCAGGTCGAATTGAGACAGCTTTCTGATTGT TATATCCAAAGCCCAAACGTGTGGAAACATTCAGTCCGGGAATTACATTATAAGC AAGATAAAAATTACCGTTAAATGATTCTGTGTCCTTATGATTTTCCTCTTTCAATC TTCCCAATGTATAACTTACGCCCTGTAAATCTGCAGGATCGCCAGCTGCATTTAC TATACTTGCCTGTGGATAAATCTGAGAACGAGTAGGCGAGTAGTCATAACATTC GTTCAATAACCCCCAAGCCGGAGATAACTGGTTTTCTATCTTCATAGCGATGTTA GTGTTGATAGTCCATTTTCCGCGCTGAAAATGTGTATTCGAACGAATATTATATC TTTTGTAATCGGAATTTATCAACACACCTTTCTGGTCGAAATAGTTCGCGGTAAG GTTATATGTCAAATCTTTCTTGCCGCCATTCGCAGTAACAGAATAATTCTGTATTG GTGCGTTATTATTGACTACATATTCATATAAACTAGAGTTGTTGAAGAAATTCACA GGATATGTTTTCAGATTAGACCAGGCCAGGTCGTCTGTATTCTGGTTTCCTTCCA TCATTCTGTTAGACATCACTTTTACAAATATACTCTCGTTGGCATCAAGCAAATGA ATATTCGAAGTAATGTGCTGTACACCATAATATCCGTCGACAGCTATCTTCATTT CTCCTTCCTTACCCTTCTTTGTGGTAATAAGGATAACACCGGAAGCACCGCGAG TACCATAAATGGCAGCCGAAGCAGCATCCTTAAGAATATCTATACTTGCTATTTC GCTACTACTCAATCCCGGGTCGCCCTCGAACGGGACACCATCGACAACATATAA AGGAGAACTGTCGCCTGAGATAGAACTTAAACCACGAATCTGGATGTTGGATTT GGCTCCAGGCTCACCAGAACTTGCCTGAACGTTAACTCCGGCAACCATACCCTG AAGAGCTGTACCCAAGTCGGAAGTACTGATCTTAGTAATCTCATCTGAGTTTACA CGTGCCACTGCACCTGTCACCTCTTTTTTACGCATTGAGCCATAACCTACAACAA CCACTTCATCCAACACTTTTGTGTCTTCCTGAAGCTTGATATTATAAATCTGACCA TTCTTGATTGCAGCTTTTACAGTTTTATACCCAACAAAACTGAACACTAAGTTACC TTTAGTCGGTACCCCTTGAAGAACGAAATTACCATCCATATCAGTAATAGTTCCA AGAGAAGTACCTTCAACTTGAACAGCTGCGCCTATAACTTCAAGGTTATTGGCA GCATCAATCACCTTTCCTTTAACTGTTATCTTCTGTGAATACATAGACAATGTATA GAAGATAAGCATCACGAACAACATGTACCTGCCATGGTACCATTTTTTCTGATTT CTCATTTGTAAAAATTTTAATTTAGCAATAGGTTATGAAATTCCTTTTATAACTGAC GCTAAATTATTTATTTATAATGGTACAAAAGGGGAGAATTATATATTTAAAAAGGG GGTAAAATTTTACCCCCACTTATATTAAGAATCCAAATCGGTCTGTATACTCTGTT CTTTGTACTGTTGCGGCAATACACCGAATTCTTTCTTGAAACATTCTCTGAAATA CTTCAAATCATTGAACCCTACATCGTATGTCACCTCTGATACAGAATACCGTCCT GTCTTCAACAGTTCTGCCGCTCTCTTCATTCTTATTGAACGTACAAAAGCATTGG CTGTTACTCCCATAAGTGCTTTCAGCTTCTTGTTCAGAACCAAGGCCGTCACGC CAAGACCTTTACATATATCCTCTATCTGGAACGAAGAGTCTGTAATGTTGTCCTC TATTATCTTTACAAGTTTCTCAAGGAACTTATCGTCGGTAGATGTAGTGCTTACCT CGGAAATCTTTATTGCCGGAACTTTCTTGTGTTGAAGAATCCGCTTCCTGTTGGT TATAATGGAATTAAGCAGCTCTTTCATTATCTTGTTGTCGAAAGGTTTAGGGCAA TAAGCATCTGCATGGAATTTATATCCGATGAAATAATCCTGCAATGTAGTCTTGG CTGAAAGCAATACTACAGGAATATGAGATGTCCTTACATCCTGCTTGATTCTCTC ACACAGTTCCAGACCATTCATGCCCGGCATCATTATATCGGATAAAACAAGATCC GGTTGCAAATCTGGAATCATGTTCCATGCCATCTCCCCATCATGGGCTATCATTA TCTTATACTTATCCGACAACAGTAATGACAACATATTACATATATCCTTATTGTCA TCAACAATCAATATAGCCGGAGATTCTCCGTCCACTTCTATGTCTATCATCTCTT CATGCTCGCACGATTCACTTCTTAACACATCAGCAAACTTTTCATCCTCCCCACT GTTGGCAGAGATATTCTCCGTAACCATGTCCCCCTCAGTTATCATAGGAATTACA ACATGGAAAACAGTGCCTTTACCTTCCTCTGATACAAACGTAATATTTCCATTAT GTATCTCTACAAGCCGCTTGGTCAGAAACAGACCTATACCGGTACCTCCTTCAG CAGAGTTTTTATTCTGACTGTAGAAACGCTCGAAGAGGTGTGTTTTCAGGTTGTC GGATATTCCGTTTCCCGAGTCTGCCACAGAGATGTTTATTTTGTTATCCTGTTCA TTGACAGTAAACGATACAAATCCTCCGGCAGGAGTATGCTTAATGGCATTCGAT ACGAGATTATAGATTATCTGTTCCATAAGATGAGGGTCGAACAGAAAGCTTATAT CACTGCGTGAGACAGAATATTCCAGCCCTACACCTTTCTGTTTTGCCCAATACGT GAACTGCTGAAATACTTCTTTTGAGAAAGACGAGAAGTTGCCATATTTGAGATTC AGACTAAGCATTCCTTTCTCGCTCTTTGAGAAGTTCATCAGCTGGTTGACAAGAC TTAACAGGAACTTACTGTTATGCTCCATTGTCTGCAGCATGCCGGCAAGATACTT GTCGGACGAATACTTGCCCGATTCAATAATCATACTAAGTGGAGAATGAATAAGT GTGAGTGGTGTCCTCAATTCATGCGATATGTTGGTAAAAAATGTAGTCTCCTTTT CAAGAAGTTCTTCAGTCTTGCGTTTTTCCATGTTTGCTATATATAGAGCATTTCTG CGCTGCACCCGTGAGGTATAATACACCTTGAACCGGTATAAAGACAAGACAAGC AATATAAAATAGAGTGTATAGGCATACCATGTACGCCAGAAAGGAGGGTTAATAA TGACAGGTATGGAAAGTTCATTCAAACTGTAGACTCCATCGCTATTCCTGACCCT CAGTCTGAACATATATTCGCCTGAAGGAAGCTTTGTGTAGAAAGCCTCACGATG AAAAGCGGAGGTGGAAATCCATGAATCATCTACGCCTTCGAGCATATATTCGTA ACCAACCTTATAAGGACTTCTGTAATCCAGGGAGCTGAACTGGAATGAGAAAGT GTTTAAATTATAAGGCAATTCAATGTGCTCTGTAAAACTTACACTTTTGTCGAAAT AAGCTGAATATGTGGAATCTGCCTCAACGCTGTGATTGAAGATTTTAAAATCAAC GAGTGTAGGACTACCGTTGAAATCTATCACATCAAAGTCATTAGGTCTAAAGACG TTAATTCCGTTTACGCCACCGAATATCATTGTTCCATCCGTCATTACTCCAGCAG AAAGTTCCATAAATTCATAATCCTGAAGACCATCGAAAATATCATAAGATCTTATT CTCTGTGTGTTGATATTCAACGAATTAATTCCTTTATTGGTAGAAATCCATAATGT TCCATCCGTGCCATTAACAATTGATTTTATTGTATTGCTGCTCAACCCGTCTGCA GAGCTAAAATTTTCAACGCAGGCATTATGGTTTTCATCCAAATCCACGATTTTCC TTAACCCACGTCCAAGTGTTCCATACCAGATATTATGATTCAAGTCTTCACATAC AGGCACTATATAGTCGAGTTCATCAAGTCCCTTGACTGAGTTCAAAACAGGATTA TCTATATACAAATCTGCAGATTCCAATACTTTAAGACCGAAGCTGGAAGCTACCC ATATATTACCCTTATGATCTTTAATGATGTTTCTTACTATCTTAAGTTCTTTATTGT CAGATGTTTTGATTTCCTTCATCACACCTGTGGACAAATCATATCTGAAAAGACC TTTATTATATGTGCCAATCCACAAATATTTTCCATCGGCAAGCATTGCGCGCACA TTTCTCAAACCTGAGATCTTTTTATAATCATTATCAGAAGTGAAACTGTAAATACC ATCGTACATCAGAGACACATACATGCAGTCGGTGTAGTTTGAGTATGCTGTTGA GTATACTATCCTGTTTGCCGTGAAAGGAATAAGTCTGGCATTACCGGTAATGGA ATTAAAATGATATAGCCCTGAGCCTTCTGTGCCTAAATATATATCAGATTTGGCA AATGTATAAACGGACGATATATGATCATTTCCTATTCCTCTGAATAAATCTATAGG TTTATTATTTTCGCGTATACTCATAAAGCCACTCTTGAAAAATCCTATCCAAAGAA TATCGTTTTTATCAAGAACTACAGTTTGCGGATAGCTGTAAGAATATGTAGCAAT AACCTGTGGTTTTGACTCGATGGCATGCAATACATCAAAAGTCAACACATTCACA GTGCTTGTAGTGGCATAAAATAATCTTTTGTTTTTATATACCATTTTTCGTATATCA CAGTTTTCCAACAGGGTACTTACCTTGCAGGTATGCTTGTCGTATAAACATAATT GATGATTTTCCAGATTTGAGTACAATATTTGAGAAGATGAGATGACTATGGCTGA AGCTATAGGGCATCCCAATAGTTTGTTAAGCAGTAATTCATCTCCATCGACGTTA CATTCGTACAGGCCGTCTTCGGAGGAGAGCATTATCGTATTATCTATTTCTATGA TGTCGGAAATGTATGGTAATTTTAATGTTGATCTTAAGACAGTATTTATTTTGCCA TTTTGAAAATCATAATTTACAAGGTATATACTTTCATCAGAGGAATGAAACCAGAC TCTGTCTTTAGAGTCGACAAGAATCTTATCGCAAGTGAAATTTTTATCAATACCG CTGTGACCAAGATTTAATGAAACGAATTCGTTCTTTACAGAATTGAACAGGAACA CTCCTCTATCGGCTGTACCTATCCACAGATTTCCATGTGAATCTTCGTCAATACA TACTATCAGATTACTGTTAAGACCGTTTGACTGATATCCGTAAACCTTAAATTCAT ATCCGTCAAACCTGTTCAGTCCGTCGTTCGTGGCCAACCATATAAAGCCTTTTGA GTCTTGATAAATACATTGCACATCATTTTGGGAAAGTCCATCAAGAGTAGTGTAC TTTCTTGTGACAAACTCATTGGATGCAAAGGATTTGCAAACTATAATCAGAACTG ATATTAAACTTAAGATTAATCTAAACATATAACTATTATTCTTTATATTTCATCAAG ATTACAAAGTTATTGATTTTATCTAAAACATCAAGTATTTACAGTAGTTAATAGATA ATTATAGATATTTTCCACTTTAGAATGCGTATCAAAATCAATCAAGAAAAAAATAA ATCTTTAACTTCATTTCATAGTATAAAACAAAAAAAGCATCGTACCATTACACTCA ATAATAGATACGATGCCCGAAAGAAATTACAGTAACAGACTGTATTGGGATTGTT CTTAAAAAGACTTATCTGTATGACTTTATATATATGTCGAGTATTTCGGTATCCGA CAGTTCATGAGGGTCCAGACTGAACAATGCACCCATGGCAGTTCGCGCATTATC AATCATCTTAGGGAAATCTTCCTTTACTATTCCCCAGTCGCTAAGCTTCAAATCG CGGACATTGCATTCCTTCTGCATTCTCACCAAAGCATCTATAAAATGTTCGGGAT TAAGGTTCTTGCATCCGGTCATAACATCTGCCATGCGCATATATCTCTTTGTCCT GTCATAAATAAAAGTAGAGAAATAGGCCTCGCTTATAGCTATCAGGCCAACACC ATGAGGAAGAGCGGGATAGTATGCGCTGAGAGCGTGCTCGAGAGAATGTTCGG AAGTACAACTGGATGTGGATTCAACCATTCCCGCCAGCGTACTTGCCCAAGCCA CCTTTGCCCTCGCTTTCAGGTTATTTCCATCCTTCACCGCAACAGGTAAATATTT ATACAGCAGTCTGATGGCCTCAAGAGCGAAAATATCACTTATTGGGGTTGCACA ATTGGCAATATAGCCTTCGGCTGCATGAAAGAATGCGTCGAATCCCTGATAGGC AGTCAGATGTGGCGGAACTGAAACCATCAGTTCCGGGTCGATTATCGACAGACA TGGGAAAGTTAAAGTGGAGCCGATACCTATCTTTTCGTTTGTTTCCAGATTGGTT ATGACAGTCCATGGGTCAGCCTCGGTTCCGGTTCCGGCTGTTGTAGGAATGGC TATGATGGGCAATGCTTTGCTGTAAGGAAGCCCCTTGCCGGTACCTCCTTCAAC ATATTCCCAATAATCGCCATCATTACATGCCATGATTGCAATGGATTTGGCCGTA TCTATCGAACTTCCGCCTCCCAAACCTATAATCATATCGCAATTTTCCTCACGAC AGATTGCCGTACCTTCCATTACATGGTCTTTTATTGGGTTAGGCAATATCTTGTC GTACACCACGGCATCAACATTATTTTCTTTCAGCAGACCAATCACCTTATCCAGA TAACCATATTTACGCATTGATGTTCCGGATGAAATGACTATCAAAGCCTTTTTGC CGGGCAATGTCTCTGTTGAAAGACGTTTAAGTTCGCCACATCCGAAGAGAATCT TCGTCGGAATATTATAACCAAAAACAAAATTATTGTCCATAAATATTATCAGTCAG TCAACTTACTATCTTAAAGCCTCATCAATCACTTTCTTGAGTTCAGGATAAGCCTC ATCTGTATCGCCCACCTGTTTTCTCAACTCACGCAGTTTCTTTTTCATGTCCTTAA GAACTTTGGCGTATTTAGGATTATCAGCCAGGTTTACCATTTCGTAAGGGTCGTT CTTCACATCGTAGAGTTCGAAAGAAACCGGAGTAGGAACAATCTTGTGGCTGTT CTTCAACCATGACATTGATTTCTGTCCGTAACGTTTGTCGTCGTAATGACGGCCA TAGAAAAGTATCAGCTTATAGTTTTCCGTGCGGATACCTATGTGTGCCGGAACG TCGTGATGAATCATGTGCATCCAGTATCTGTAGTAAACAGCATCCTTCCAGTTTT CTGGCTTTTTGCCTTCGAACACAGAGGCAAAGCTCTTTCCATCCATGTATGAAG GTTCTTTGCCACCGACCATCTCTATAAGAGTTGGAGCAAAATCAATGTTGTTAAT CATCAGGTCCGACTTGGCTCCCTTGTAAGGACATCTCGGGTCGCGGACTATGAA AGGCATTCTTTGAGATTCTTCATACATCCATCTCTTATCCTGCAGATCGTGTTCG CCAAGCATCATACCCTGGTCGCCTGTATATACGATAATGGTATTTTCCCAGAGTC CTTCCTTCTTGAGATAGTCGAAAAGACGTTTCAGGTTGTCATCCACACCCTTTAC GCAACGCAGATACGATTTCAGGTAATGCTGGTAGGCAAGGTATGTATTCTCCAT TTCATCACCTGTATTGCACTTATATTCCATTACATAATTGCGGATTTCATGACGGC TTGAGACAGAAGTTCCGATGAAGTGACGAAGTGAATCGTTCTTGCCTCTTGTGC CTTCGGAGCCCCATTTGTCTGTATCGAACAATGACAATGGAACAGGCACTTCCA CATCGTCAAGATAATATTCATAGCGCGGTGCGTACTCGAACATATCGTGCGGTG CCTTGTAATGATGCATCATGAAGAAAGGTTTGGACTTGTCGCGTCTGTTCTTCAA CCAGTCAATAGCAAGGTTGGTCACGATATCCGAGGAGTAACCCATTTTCTTTATC TGGTTATTAGGCCATTTCTTGTCAGTTACGTCACTTGTAAGGAAAATAGGGTCGA AGTATTCGCCCTGTCCGCCATGACCGTTGAATACAGAATAATAGTCGAAGTGCG ACGGTTCGCATCCCAAATGCCATTTACCGATCATGGCAGTCTGATATCCCATATT ATGGAACTCATCAACCAGATATTCCTGGTCCGGCTGAAGCACTTCATCCAAAGT GAGCACCTTGTTACGATGGGAATACTGTCCGGTCATGATACATGCACGGCTTGG GGTACTGATGGAGTTTGTACAGAAACAGTTCTCGAAGAGCATACCGTCCCTTGC CAGTTCATCAATTGTAGGAGTAGGGTTCAGTACTGCAAGACGACTTCCGTATGC GCCGATAGCCTGCGAAGTATGGTCGTCCGACATGATGTAGATGACATTCATCTG TTTCTGCTGTGCTGCGACACCAACACATACAGACAGGAATGGCATAACAGCCAT TCCCTTCATTATATTATTTTTTAAATTCGTTTTCATAAGTCAGATTATCATTGAAAT AGAACTTGCAAGACATATCATCGAATGATTTTACGTCCTTATTCTGCATTTTAACC CATTGTTCTGATTTAGCCTTGACAGCGACCTGAGTTGAAACCTCATTACCGTCGA CTACACTTTTAAGAGTGACATTTGCATCCTCTGCATTATGGTTTGCCACACGTAC AGTGATAAGGCATCCGTTATCAACCTTATCGTATAGCGGTTTGGAAACCACCGC CCCTTTAAGCTTAATCTTGAACACATGTGCATATTCAGTAGGTTTGTTCTTAGGG AAGTTTACTACAAGACCCTCGTCAGTCATCTTATAGTCAATCTTCTCTGAGCTTC CAAGCATTTCAACCGACTCAATTTCCACGTTCTGGCAATACTTAGGAGCAAATGA CTTGATAGTAACACTACCATCTGTCCAAGCCAGAGACACGGCATAGAGGTTATT GTCGCGTGTAGTAAAGCGAATGTCGTCCGCTGTATATTCAGTTTTTGTATTGTCT GTCATATAACCTGCGGTGCCTGCGTTATGTCCTTCGAAAGCAATCACCCATGGT CGTGAGCCATAAATAGCCTCACCGTTAGTCTTCAACCATTTACCTATCTCGGCAA GTACGTTCTTCTGTTCGTCTGTAATAGTACCGTCGGCCTTAGGACCTATATTCAG CAATAAGTTACCGTTCTTGCTGACAATATCAACAAAGTCGTCGATGATATGGTCA GGACTCTTGTTTTCCTCGCCCACACAATAGCTCCACGATTTCTTGCCTACAGAAG TATCAGTCTGCCATGGATATTCACGGATTCTGTCGCTCTTACCTCTTTCTATATC GAACACCTGGATATTGTCGCCATATCCGAATTTAGTGTTAACCACAACTTCTTTA TTCCAATCAAGAGCCGAATTGTAATAATAAGCCATGAATTTATAGAAAGTAGGCT GGAACGGATATTTTCCCACAGTCCAGTCGAACCATATCAATTCAGGCTGATATTT GTCGATAAGCTCGTATGTATGCATAAGGAACTGACGGCGTGAACGTTCGTTCGA GCCTTCATACTTACCACAATAAGGTGTCATACCCTGACCTTCGGGCTCATGCAG TCTTTCGCCATACAGAGTGATTGTAGTGTCCTGAACATCAGAAGGAGTTTCCATT CCATATTCATAGAACCATGCATTCTCGCATCTGTGAGAAGAAAGTCCGAAACGC AGACCGGCTTTCTTGGTAGCTTCCTTCAATTCGCCGATTATATCCCTTTTCGGTC CCATATCCACAGCATTCCACTTATTGAAAGTACTGCTGTACATGGCAAATCCGTC GTGATGCTCGGCCACCGGAACAATGTATTGTGCTCCAGATGATTTTACCACTGC CAGCCACTCGTCGGCATTGAAATTTTCGGCTTTGAACATAGGGATGAAATCCTTA TATCCGAATTTGGTCAAAGGACCGTAAGTCTGTACGTGATACTTATTAATAGGAT GACCTTCCTTGTACATCCAGCGGGAATACCATTCACTGCCGTATGCAGGAACGG AATAAACTCCCCAGTGGATAAAGATACCGAACTTGGCATCCTTAAACCATTCAGG AATAGTGTAATTTTGAGCAATCGATGCCGAATCGGCCTTGAACACATCAGTACCT TTTAAAGATACAGTAGAATCTACATTAGGAGCGTATGTAGAATTGCACGACGCCA ACAGGCTTAATGCCGCAACTCCTAAAACCGTTTTCATGGATTTCTTATTCATAATA ATCTTATTACATTAAATAATGACATTAATTTTTTCTGTAAGCAAAGATACACTTGA GTTCCATTTACAATAAATAATTTAATTACTATAGTAAGGGGTAAAATATTTACCAC CTATTATTGAACAAATTTACCCCCTCTCATATATGATAATAAACTGCCAATATCGA ATTACAAGTAAATATATATTTCAACAAAAAAGGTTTAGCCTATTATTACACAACAA TTTCACCCTAAGAATAAAATATATATAGAGTAAATTTGCCAATATAACAAACTGTA AAAACAAATTTATGAAAAACTATTTGATTTACTTACTCGCAGCAGTATCGTGTACA ACTGTAGCAGACCTAAATGCTCAAGTCAGTACAAAAACAGGTAATGAAACCACA GAACTTACAATTCCGAAAAAGTTCTACAAGGACAGCATTGATTTCAGCAATGCTC CGAAAAGACTTAACAACAAGTACCCTCTTTCCGACCAGAAGAACGAAGGCGGAT GGGTTCTAAACAAAAAGGCCTCTGACGAGTTCAAAGGAAAGAAGCTGAATGAGG AAAGATGGTTCCCGAACAACCCTAAATGGAAAGGAAGACAACCTACTTTCTTTG CAAAGGAGAATACTACATTTGAAGACGGCTGTTGCGTGATGAGAACTTACAAGC CAGCAGGATCACTGCCCGAAGGATATACTCACACTGCCGGTTTCCTGGTAAGCA AAGAACTTTTCCTTTACGGATATTTCGAAGCAAGACTGAGACCAAACGACTCGC CATGGGTTTTCGGTTTCTGGATGTCGAACAATGAAAGAAACTGGTGGACTGAAA TAGACATTTGCGAGAACTGCCCCGGCAATCCTGCCAACAGACATGACCTGAACT CGAACGTGCATGTATTTAAAGCTCCAGCAGATAAGGGTGATATAAAGAAACATAT CAACTTCCCTGCCAAATACTATATACCATTCGAATTGCAGAAAGACTTTCACGTA TGGGGACTTGACTGGAGCAAGGAATATATCCGACTATATATAGACGGAGTACTG TACAGAGAAATAGAGAACAAGTACTGGCACCAGCCATTACGCATCAATCTTAAC AACGAATCGAACAAATGGTTCGGAGCCTTGCCGGACGACAACAATATGGATTCT GAATATCTGATAGATTATGTAAGGGTGTGGTACAAGAAATAAGAAATAACATAAT CTGAAATTATAAAAGGCAGTCTTCATTATCAGTATGCTGATGATAAAGTCTGCCT TTTTAACAAGAAGATAAAGATTTTAATCTGCCCTATCACTCATTTACTTCATCCGG ATACTCTGTAAGCGAGTTTCCCGAATTGCTTATTTCAATAGAGCCGATAGGAAGA TAATTGAACTTCTTGCTCCATGCAGAGATACCATAATCTCTTCTAAGAATAGGCA TCATGACCTCCTCGGCACGTCCTGAGCGGACGAGGTCAAACCATCTGTCACCC TCGCATGCCAGTTCACAACGACGCTCATACCATAGAACATCAATTACGCTTTTAA ATCTGTCAGGATACATCTGCATTAGCTTGTCAACATCAATATAACTTCCGTCGTC TGCATGAACATGCTTCTTTCTGAGTTCATTTATGTAATACTTCGCTTTTGCTTCAT CAGGATTAGTACCTCTGAGATATGCTTCGGCAAGCATCAGATACACTTCACCATA TCTGATGACCCTTACGTTTCCAGGCTTGTTTAGATTGGGGTTTCCTATCATATCG TAATTTTTGAAAGGAGGATATTTCTTCTGGGCATATCCCTGGAAATCAGGCCCGT AAGAGCCTGTCTCCCAAACAACTTTTTTTGATTCATCCTGAATATTGGCATTAGG TTTGGTTACAAGTTCATCGTAAGTAAATATCGCCGCATCACGACGCACATGGTCA TCCGGAAGGAAATAATCATACAATTCCTTAGTAGGCAGACAAAAGCCATATCCAT TATCATAATCAGGACTATTTTTCAACTGTCTCGGTCCGCAGAAAGTCACCCACAT AGCACCTTCGCCTGCATCAATATTACCCCAGTTTGTATTACCAGATTTGGTAGAG GTCTGTATTTCAAATATAGATTCCTCGTTATTCTCCTGATGAGCCGCAAACAATTT AGAATAATCATCCGTCAGAGTATAATTACCACTTGAAATTACATCCTCCAATAAA GGTTTCGCTTTGTCAAAAATCTTAGCATCATCGTTGCTCCAGTCAGCCCAATAAA GATAGACCTTGGCCAACAGGGCTTGAGCCGCAGTCTTGGTAATACGTCCTTTCA TTGTGTCCGGGAAATTATCCTTTAGAGAAGGGATAGCTTCAAGAAGATCTTTCTC TATTGCTTTATTTACATTTTCGCGAGTATCTCTCGTAAACTTGAATCCTTCAGGAT AAAGAGTCTCAAGACTGATAAAGCATGGACCATAATATCTCAACAATTCAAAATG ATACCAAGCACGTAAGAACTTAGCTTCAGCTTTATAAACTTTAGCTTCCGGACTG TCATACTCTGAATTTATTACAAGATTACATCTATATATACCACGGTAACGAGTTTT CCACAAATTATCGGAAATAGAATTGACACTCGTATTTGAATAATCCTCTATAGCC TGCATGTAAGGCTGATCCTGATCAGAGCCACCACCAGTACGAGCATTATCCGAA CGGATTTCACCCATAGGTACAATGGAAGCAAGTGCATTACCCGAAGCACCACCT ATGTGAGCTAACGGATCATAACAAGCAGTAAGCGCTTTGAACATCTGTTCATCG GTCCTATAAAAAGAACTTTCTGTTTCGGACATTATAGGAGCTGTATCCAGGAAAC TGTCGCTGCAAGATGATGATGCAATAGCAGCAAACATGAGGACAAGAATATTAT TATGTATTTTCGACTTCATAATTTTCAATTTTAGAAATTAAGACTTAAACCAAATCT GAATGTACGGGCCTGAGGGTAAGTACCATAGTCAATACCTGTGCTAAGAATATT GCCACCTGCCATATTTCCTACTTCAGGATCCATAAACGGATAGCTGGTGAAAGT GGCAAGATTATCAATTGCTGCATAAATTCTTGCTTTATTCAGCATCAACTTGTTTA TTAATTTAGTTGGGAATGAATAGCCTACCTCAAGTGAAGAAATCTTTAAATGCGA ACCATCATAAAGATAAAAATCGGATGGTTTGCCAAAGTTTCCATTAGGATCTTTG GATGAAAGACGAGGCACTCCATTATCATCACCTTCTTTCCGCCATCTGTCAAGAT AGAATGATGGAAGGTTGCTGCGTCCGTATGCTTCCTGTCGGTAAATATCAGAGA AGACTTTATATCCAGCTTTTCCTGTTAAGAAGATTGTCATATCAATACCTCTCCAG TCGGCACCTAAATTCAAACCGAATGTCCATTTTGGCCAAGGATTGCCACAATCG GTTCTATCTTCATCTGTAATCTGCCCATCGTTATTTGTATCTTGCCATATAAAGTC ACCCGGAACGGCATCAGGTTGTATCACTTTACCGTCTTTTGATTTATAGTTCTGT ATCTGCTCTTCATTTTGGAATATTCCTAAGTTCTTATAAAGGCGGAAATAACCCAT AGCATGACCTTCCTCCATACGCGTTACATTAACAGATGTTCTCCAGCTACCACCA TCAGTATATCCATTTACATTTCCTATCTTTACAACCTCATTTTTAAGATATGAGGC ATTTGCGGAAATAGAGAAGTTGATTTCGTTCCAATTTTTATTAAATGTCATCTGCA TTTCCACACCCTGGTTTGTTATATTACCAAGGTTTCTAAAAGCTGCATTATTACCT CTAATGGCTTCAACTGTTGGCTGGAACAACAAATCCTTAGTACTTTTTTTAAACC AGTCGAAACTTGCTCTAATCATACCATTATAGAATGTCATATCGGCACCAACATT AAATTGTTCAGAAGTTTCCCATTTCACGTCTGGATTAACAAGGTTATTAGGAGCA GATCCCACAGTGATGGCATTACCAAACGTGTAATTATAATTATTGCCAATAATAG AAGTATAGGAGAATGGAGAAATTCGCTCATTTCCGTTCTGTCCCCAAGAGAATCT AAGTTTGAAGACATCAAAGTTCTTAATTTTCCAGAATTTCTCATTTGAAACATTCC AACCTAATGAAACGCCCGGGAAAGTAGCATATCTGTTATTGGGACCGAAATTTG AAGACCCATCGCGTCTGACCACAACTTCCGCCATATATTTTTCAGCATAATTATA GCTTAGACGAGCAAAATATGAGAACATACTATGTCTAGGATTAGCACCGCCACT ATTAGCTGATGTCATAACATCACCAGCATTAAGATACCAGTAATTCTCATTGGTC ATTGCTTCATTTGGATATTTATTTCGTGTTCCGGCCATAAACTCATAAACATCTCT TGATGCAGAAGTACCTAACAGGACAGATGTAGAATGTTCACCAAAAGATTTTTTA TATCGCAATGTATTCTCCCACTGCCAACTACTATTAGCATTTGTACTTTGTTCTAC CCTAGAATTATCTTCTTTACATTCTGCAGAATGAAAAAACTTTGGTGCAAACATTC TTCCACGGAAATTCCGATGATTAATACCAAAATCTGTGCGGAAAACAAGGTCTTT AATAAAAGTGATCTCAGCATAAACATTACCAAAAAATTGCTGGGTAATATTTTTAT TCTTAGGTGCCTCATCCATAAATGCAATAGGGTTCCACATACGGCTATAAGGTAC AGGAGAGACTCCATATCCGAAAGTATCGTTGCTATTCTCATCATAAACCGGAGTA GTAGGATCAATATTATAGGCGTATGATATCGGATTATAACCATTGATACCGGTTG CCACTCCACTATTCTCTATATATGCATAGTTGACGTTTGCACCTACACTTAAGAA ATCATTTATAGAATAGGAACTGTTCAGCCTTGTGCTGAATCGTTTGTAAAATGAC GCATCTTCACCGATAATACCATTCTGGTCTAGATAATTCAATGAAAGCAAGCTTG AACCCTTATCACTGCCAAAGTTAGCAGTAATGTTATGCTCAGTAACAGGAGCTGT ATTCAATATTTCATTAAACCAGTCTGTATTATAACCTGTTGGAGCAGTAGGTACA CCACCGGCAAGCGGCATATCATCATTGTCGGCAAACTCTTTCATCAGCATAATG TACTGTTCATCATTCAGCATGGTTGGTTTCTTTGCTACTGTAGAGAAACCATAGT AACCATCATAAGCAAGCGATGTCTTTCCTTTCTTTCCTTTCTTTGTGGTTATAAGG ACTACACCATTAGCGGCTCTGGCACCATAAATAGCAGCTGAAGTTGCATCCTTC AAGACTTCCATGCTTTCAATGTCGTTGGGATTTACACTGTTCATGTCGTCCATAG GCAGTCCGTCAATTACAAAAAGAGGATTAGAGTTTCCATTTGTACCAACACCACG AATTACCAGCTTCGGTGCTGTTCCTGGCTGACCGGAATTTGTCACAACGTTCAC ACCACTAACCCTACCGCTCAATGCATTCACGGCATTTGCTGGTTTAGATTGCAAT AAATCATCGGAATCGATGCTACTGATAGCACCTGTTACAACACTTTTTTTCTTAAC CTCATATCCTATTGCTACAACTTCCTCGAGTGCAATGGCAGATGTTTTTAATTGA ACGTCTATCTTAGACTGACCTTTATACACTATATTCTGTGTATCATATCCTACGAA GCTATAAATCAATGTCGATTCCATTGGTACATTTTCCAAGATATAATTTCCGTCCA AATCAGAAATAATACCGTTTGTGGTACCTTTAACTAAAATACTTGCACCTATCACA GGTAAACCATCGGAGTCTGTTATACAACCGGTAACTTTCCCGTTCTGTGCATTTA ATGGTAAACTGAACGTTATAAGAATCAGCATACACATTAATGATAGTGTTCTGTT CATAATCTAGAGTTTTTTGTAATTAGTGTTTTTCTTAAAATAAAAAGTTTTGTTCTA TCAGTTGCGCGCTACTTACTGACACTTGCAAATATATATACTATGTAATATAACCA AAGGGGGAAAATTTCATTTAAATAGGGGGGGGAAATAGATTAACTAAATATTTTA AGGAAAAATGGCTGTTAGAATCCATTCCCAGACTCCAACAGCCATTTTATCACTA ACAATCGCCTGTTAATCAATATATTTTTCTGCCCATTTCCTTAAGATTTGCATCCC TGCCCAGTGGAACAAAAGTAAATCCGTATGAATAGCTTCCCTTCAGAAGACGCT TGTCTATTGAAGGACGGGCTTTCAGACTCCAGCTATCTGTTCCGCCCACTCCAG CCTGAACCAGGTCGATATTAAGAGTATTAGAATACAAGTCCTTTTCAAGTTCATT TATATGTTTAGCCTTATCAATCGCATTCTGCGACATCTCCCACACTGAAACAGAT AGGGGTTCATCGCCGACAATCATCACACCTGCCTTATCCGACTGCAAGGCAAAC CATCTCACGTCACAACGGTTTCCGTTTTCCTGCGGCATTACATAGTCAAATCCCA GAGCGGACACCTTGCAGTTATATATAGACACCATTGCAGAGGCTTTTCTGTCGG AATAGTTTTCCCATGGGCCACGTCCATAATATGTCACATCCGACAAACGATTGGT ACATTCGCATTGCAATCCTACGCGCAACATTTCTGATATTTCAGGAGACTTCATC ATTGAATAATGAACGCCTATTGTTCCGTCTGCTTTTACTTTATAATTCAAGGTAAG TCTCAGTCTTTCATCTATAGCCTTTAGCACCTTAACCTCAAGATTGCCTTCCGATT TGCGTACATCTATAGAAACTGTCTTTAGCTTTAATGGAGCATCTTTCCAGAATGC AAACAGTCTATCGACCTTCCATCCTCGCCAGTCATTGTCTGTTGACGCTCTCCA GAAGTTTGGTTTCAGAGCAGATGTGATGATACTTTCATTATCTATCTTATACTGAC TGATATAACCATCACTGATATTCAGATAAAAGTTCTTTCCCTTCACGCTGATGTCT TTCTTGTTATCTGAATCGATTTCCATATCCAATGTAGTATCAACGCATTCTACTAT CTTTGGTAAAGAAAGATACTTAAACTGTTCCCAGGCAACCTCGTATCCAGCTTTG GCATACAGATTGTCATTCTTGAGCCTGGCACTCAGGAATAACCAATATTCCGCA CCGTCATCGGCCTTGAAATTCTGAATAGGAAGTTTTAGTTTACAGCTCTCACCAG CTGGTGTTGTCGGCACAATAATCTCACCTTCCTGCAATACACTGTCTTCGTCCTT CAATTGCCAAAAATAACGATACTCATCTGTTGAAAGGAAGAAGTTTCTGTTTTTTA CAGTTATCTCTCCACTATAGACATTATCAGTTGTAAATGATACAGGAGCAAACAC GTACTTGCATTCCTCAGTAGCAGGTTTAATGGAGCGGTCGGCACTGATAACACC ATTTATACAGAAGTTTTGGTCGTTGTGCTCCCCTTTCTCATAGTCACCACCATAA TTCCATGATTTCTTATTATATTTCCGTTCATTATCCAGCAATCCCTGGTCTATCCA GTCCCAAATATATCCGCCGGCAAGCGCATCATGAGAACGTATTGCATCCCAGTA TTCTTTCAGCCCGCCGGTAGAGTTTCCCATAGAATGTGCATATTCACACATTATT ATCGGACGGTTCATGACCGGATTCTTAGTCATTGCTATAAGCTCATCGACCATA GGATACATACGGCTAATGACATCGACGTATAAAGGATCATCGGGATTGGCATAC ACACAAAGCTCTTTCTTTGCCGGTTTGACATCTTCGTTCACATTAAAATCTATCTC ACTAGTAACGATTGACGCTTCCTTACGTCCGATAGGTTTGTATAAAGGATTTTCC GGCTGTCCTTGCGCCCCCTCGTAATGAACAGGACGGGTTGGGTCATAATCTTTC AGCCATCCTGACAGAGCTGCATGATTAGGGCCGCATCCAGACTCGTTGCCCAA CGACCACATAAACACAGAAGGATGGTTCCTGTCTCTCACAGCCATTCTTACCAC TCTCTCCATGAACGAGTTAGCCCACTCAGGCCTATTGGACAGATACCCCCTTTG ATGATGAGTTTCAAGATTAGCCTCATCCATTACGTATATACCATACTTATCGCAC AGTTCATAGAAATAAGGGTCGTTAGGATAGTGCGATGTACGGACTGTATTGAAG TTATAACGCTTCATAAGCAGAACGTCTTCGAGCATCTCATCACGTGTAACGGTCT TACCTCCGGTCTCGCTATGGTCATGGCGGTTTACACCAATGAGTTTAATAGGAG TGTCATTCACCAGAATCTGATTACCTGTTATTTTAATATCCCTGAACCCTACCTTA TTACTTCTCGCATCCACCACGTTGCCCTTTTTGTCTGTGAGCTTTATAACCAAAG TGTATAGATAAGGGTGTTCCGAATTCCATAGTTTTGGCTTAGAAACAATTCCCTC CATCATTCCGTAATAAACATTATCACGCTGAGGATAAGGTTCGTTCACCACATAA TCGGCAGTAACGGTAATGTCTTTTCCAAACACCGGTTTCCCATCGGCATCATATA ATTGGGCTGACAGATTCCATCCCTTCAAATCATCCATATTCTGATTTGTTATTTCC GGACGGATCTGTAACCGTGCTATATTCTTCCGGAAATCGATGCGTGTCCTTACT CCATAATCATATATTGCCACCTGCGGAATGGACATGATATATACTTCACGATGGA TACCAGCCATTCGCCAGTGGTCGGCATCTTCCATATAACTTCCGTCGGTCCACT TATACACTTGCACCGCCAGTTTATTCTCCCCCTTCTTAACGTATTCGGTAATATC AAATTCAGTAGGCAGACAACTGTCTTCGGAATATCCCACCTTCTGTCCGTTTATC CATACATTAAATCCCGAATAGACGCCTCCGAAATGGAGTATAATCCTGTCGCTCT TCCACTTGTCAGGAACAACAAACTCCTTGATATAACACCCCGTCTGATTATTCCT GTCAATATATGGCGGACGAGCAGGGAAAGGATAAATAGTATTTGTATATATAGG ATAGCCATATCCCTGCATCTCCCAACATGAAGGAACAGGAATAGTTTTCCATGAT GATGAATTGTACTCCACTTTATAAAAACCGGCGGGAGCCAATGCCATATCCTCG GAAAAGTTAAACTTCCATTGGCCGTTCAACGACATATACTCCGATTTCTCTCTGT CTCCATCCAAAGCCCAATCCACTCTCCGGAAAGAATAAGTAGTACTGCGGGAAG GCAAACGGTTAATTCCGTTTATGGTCTGATCCTGCCATACATTCTGATTGTTTCT CCACTGATTGGCACCGTTGTCCGATGCAGACAGAAATTGCATCATGAAAAATAA CACAGAAAATGAAAAAATAGATTTTAAGTTCAAGTTCATAAATTCGCATTTTAAGT TTCTATGCAAATATATAAGTATAACGAACAATGAATAGGGGGTATTTCTATCTATA TAGAGTGGTATTTTTACATATGAGCTAAAACTTAAAAAAAACTGTCAGTATTACTA TGCTATGTAGCACTCTATATGAAAATATTATATATTCCCAAGTCAAAAGCCTTTTC AAACAATTTTTATATATTCTCATCCTATCCCTTCCATCAAAGATAAATTCCAATCCT GATTTGCCAGCCGCATTTATTCCTTTTTTCAGGAGAATTTTCTTTATGGCTATCGC CATGAAAATTCACCTGAAAAAGAATGCGGCGGCAAACGGATTAGAATTAAAGAA AAGATTACAGGGATTAACTGCGACCGACGTGACGCATAGCCGTAATTCAAAGGC GGCTATCCTTATATTCCATATATGACCTCACAAATACTGTGAAAATCCACTTTCCC CAATAACAAAACATAGCCTGCCATATCAACACCCAAAATAAGACAGGGATTTCAA CTCCCTCCGATCTGCATAGTCTGGTGGCTTCGCTATGCTTTTACTCCTACATCCA TTTTTTTTCTTTCTTTTTTCCTCTGTTCCCGTTCTTTCCTATCCTTCGTGTGACATT TGATGACACCTGATGACATCTAATGTCATCTATTTGTAAATCAATTGTTTACTCAA TTTATCATCTTACATTTGGACTGTGAAACAAATCAAGTAGTCACTCAAAACAAAAG ATTATGGCACAAGAAAACAGTCCTGACAAGGAAAAAAGGCAAGGCCGGACAAA GAAACCCGAAAAGCCTTATGTGGAACAAATTGACGAGCTTCTGCTGGTACATAA CAAGAATGACCCAAAGGAAGGTTTGGGAGTAATCAGCAAGATGGACGAGAAAG GCAATTATCAGACGGTTACACCGGAAGAGAAGAATGAGAACTCATTCCTGAAAT TCGACAAGAATTCGAGTATTCTCGAAAACTTCATCAAGAATTTCTGGAGCCAGCT GAAGGAGCCTACGCATTTCAGGCTTATCCGTATGACCTTCAATGATTACAAACA GAACAAACAGGCTCTCAAGGACCTGGCCGAAGGCAAGAAGACAGACGCGGTAA AGGAGTTTCTGAAACGCTATGAAATCAGACCGAAAGTAAACAATCAGAAAAACA GTCAAACAAAAGAGGAGGAAACAACAATGGCAAAGAAGCAGGAACAGACAACG CAGGCTCAGCCTGAACAGGTATCACAGGTGGAAGCTGCCGCACAGGGGCGCG AACAGCAGGAACCGCAACGCCAGCAGACACCCACGTACCGCTACAACGAGAAC ATGATTAATTGGGAGGAACTGGGTAAGTTCGGTATATCCAAAGAAATGCTGGAG CAGTCCGGACAGCTTGACAGCATGTTGAAAGGATACAAGACCAACAGAACCATG CCGCTGACACTCAACATTCCTGGGGTACTGACCGCAAAACTTGATGCACGCCTT TCGTTCATATCCAACGGCGGGCAGGTCATGCTGGGCATCCACGGTATCAGAAA GGAACCTGAACTGGACCGTCCTTATTTCGGACATATCTTCACGGAAGAGGACAA GAAAAACCTGCGTGAAAGTGGAAACATGGGACGCGTGGCTGACCTTAACCTGC GTGGCAACACGACAGAGCCGTGTCTGATTTCCATCGACAAGAATACCAACGAAC TGGTAGCCGTACGGCAGGAGCATGTCTATATCCCGAATGAAATCAAAGGGATAA CCTTGACTCCGGACGAAATCCAGAAACTGAAAAACGGAGAACAGATATTCGTAG AGGGAATGAAGTCCAATCAAGGTAAAGAGTTTAATGCCAATCTGCAATATAGTG CGGAAAGAAGAGGCATCGAATTTATCTTCCCGAAAGACCAGGCTTTCAACCAGC AGACGCTTGGCGGTGTACCGCTTTCCCCCATGCAGCTCAAAGCGTTGAACGAA GGACACACCATCCTTGTAGAGGATATGAAACGAAAGAACGGCGAACTGTTTTCT TCCTTTGTTACCATGGACAAGGTTACAGGCGGGCTCCAATATACGCGCCACAAT CCGGAAACGGGAGAAATCTACATACCAAAGGAAATCTGTTCGGTACAGCTCACA CCGGAGGACAAGGAAGCGTTACGCAAAGGGCAGCCCATCTATCTTGAGAACAT GATCAACCGTAAAGGTGAGGAATTCTCGTCATTCGTCAAGCTGGACCTGGCAAG CGGAAGACCACAGTATTCCAGAACTCCGGACGGTTTCAACGAACGACAGGCAC CAGCCATCCCGGCTGAGGTTTACGGACACCTGCTTTCGGCACAGGAAAGAGCT AATCTTCAGGACGGAAAGGCTATCCTCGTAACGGGTATGAAAGGTCCCAACGG CAAACCGTTCGATTCCTATCTGAAAGTAAACGCAAACACCGGACAGCTGCAATA TTTCCAGGAAAATCCGGATGTGCGCCGCAATACTTCACAGCGTGCTTCACAGAC TGACAATACCCAGCAGCAGGAACAGAAGAAGGGAGCAAAACAGGCTGTCTGAC CTGAACGGGATTCAAATCATTCAAATCATCAATTACTAAAAAAGGAAAGAACATG AACAAGACCAATCATCATATCTACAAGACTGAACAAATCGACTGGGAGAAACTG GAATCGGTAGGTATCAGCAGATCGCAAATTGAAAAGGACGGAAACATGGACCTG CTCCTTCAGGGAGAGGAAACCAATGTCATGTCCATTAAAATCAAGACTCCTGTAT TTTCACTGACCATGGACGCCACACTCAGTCTGATTGAAGACGAGAATGGAAATC CGGTCATCAGCGTAAACGGTATCAACCCTTCAGGTGAATAAATAAGAAACCATA ATGTATCATCTCTCTTTCCATACGGACTTACCGTATGGAAAGAGATAAAAACAGA ATTTATCATGATTGCCATATTAACAGACAAACCAAGTGTAGGAAAAGAAATCGGA AGAATCATCGGTGCAACCAAAGTAAGAAACGGATATGTGGAAGGAAACGGCTAC ATGGTTACATGGACTTTCGGGAACATGCTGTCACTGGCCATGCCGAAGGACTAC GGAACCCAGAAGCTGGAACGGAATGACTTTCCTTTCATCCCGTCCGAATTCGAA CTGATGGTACGGCATACACGCACCGAGAACGGATGGATACCGGACATTGATGC CGTGCTCCAGCTTAAAGTAATCGAGAGAGTGTTTCAGGCATGCGATACCATCAT TGCGGCTACCGATGCCAGCCGTGACGGGGAAATGACATTCCGCTATGTCTATC AATACCTGAACTGTACACTGCCTTGCTTCCGTCTGTGGATTTCCTCTCTTACCGA CGAGTCTGTGCGTAAAGGCATGGAAAACCTGAAGCCGGACAGTTGCTACGACA GCCTGTTCCTTGCTGCCGACAGCCGCAACAAGGCGGACTGGATTCTCGGAATC AACGCCAGCTATGCCATGTGCAAGGCGACGGGCCTTGGCAACAATTCTCTCGG ACGGGTACAGACACCGGTACTGGCTACCATCAGCAGACGCTACCGTGAAAGGG AGAACCATATTTCATCGGACAGCTGGCCCATCTACATCAGCCTGCAAAAGGACG GCATCCTTTTCAAGATGCGCCGCACACAGGATCTTCCCGACAAAGAATCCGCTA CAATGTTTTTCCAGGACTGCAAGCTGGCACATCAGGCACAGATTACAGGTATCA GCCACAGCGTTAAGGAAATACTTCCACCGGACCTGCTTGACCTGACACAACTTC AGAAGGAAGCGAACATCCGCTATGGTTTTACCGCATCAGAGGTGTATGACATCG CCCAGTCTCTTTATGAAAAGAAACTGATTTCCTATCCGCGGACTTCCAGCCGTTA TCTGACGGAGGATGTGTTTGACTCGCTTCCACCAATCATGGCGCGTCTGCTTTC ATGGGAGCTGTTCCCTGCAGCTAAAGGAACTGGAGGTATTGACATATCCAATTT GTCCCGCCACGTAATAAGCGCAGAAAAAGCCAATGTACATCATGCCATCATCAT TACAGGTATCCGTCCCGGAAATCTGTCCGAAAAGGAAATACAGGTTTACAGACT TGTAGCCGGAAGGATGCTTGAAACATTCATGGCTCCATGCCGCATAGAAACGAC AAATGTTGAAGCGGTTTGTGCGGCACAGCATTTCAAGGCCGAACAAACAAGAAT CATTGAAGCCGGCTGGCATGATGTGTTTATGCGTTCCGACATGGTTCCAAAATC AGGATATTCTGTCAATGAACTCCCCGAAGTGGAGAAAAGTGATACTCTGAATGT ATGCGGATGCAACATGGTACACAAGAAACAGCTGCCGGTAAATCCGTTCACGGA TGCAGAACTGGTGGAATACATGGAACAGAACGGACTGGGTACAGTATCCTCAC GTACCAATATCATCCGTACACTGGTTAACCGTAAGTATATCCGTTATTCAGGGAA ATATATCGTTCCGACCCCGAAAGGCATGTTCACCTACGAAACCATCCGTGGAAA GAAAATTGCGGATACTTCACTCACCGCAGACTGGGAAAAACAGCTGGCCGGAC TTGAAAGCGGAATGATAACCGGACAGGACTTCCTGAACAGGATCAGGACTCTCG CCAAGGAAATGACTGATGACATTTTCAACACCTATTCCACAAAAGAAGAATAACA TCTATACCTAATCAACCAAGAGAATGCAGGCCGGAAGGTCTGCATTTTTTTGTAT CCGTACAGAAAAGAATCTGTTTTTCCGCTTTTAAGCGGCAAAGGTCTTGGATTGC CTGCCTTTTGCCGCAAGGCTGCCCTCATGGGCTTGGCTGGACAGGAAAAAATC ATCCTCGCTGCGCTCCGGTATTTTTTCCTGCCAGGCCTTGCGCAAAAAGGCAAT CCAAGAGGCCGGAGGCCTATAAAATCGGGAAAACACATCCCGATGGGATTATTC ATTCATAAAATTAAGGATTATGAAACTACAGATTATCAGAAAGATCGGCAGACAT GCAACAGCGATATTCCTGATTACCGGAATATGTCTGCTGACAAGTAAAGGGATT GTCCCTACTGGGATGATTACGCTGCTGTTGCTTGCAGGAGGGTTCATCGGTTTT CTGTTCAGGATACTGGTCATTATTTTCAAGATTCTTATTCTTCTGTTCATTGTAGG ATTATTTGTCGCATAACCCAAAATATAAATATACATATATGGAAACAGTTGCTATA ACCTCACAAGCTCCTGTCATGCCGGCTGTATGGCCACAGAACGAACATATCAGA CCGGTTAAAAGACGTCTGCCCAATACAGTTGATGAACCTAAAAATATCGGCTACT ATCTGGAATCGCTACGTGATATTTCCAGCAATCCGGACAGAGAGAATATTCTGA AAGAATTCTTCAAGGAAACTTATGTATAACCATAAAATTTTTCAATTATGTTTTTTC AATCAATTTATCAGATGATTACAGCAGGTACGGATCTGAATATCAATATCCGTAA AGTGGACAACAGCCTGAGCGTAGCAGTCATGCCAAGGCGGAACAGCCTGAAAG AGGATACGCGACAGAACATGGTGCCACTGATCGTGAACGGAACACCGGCAGAA CTGGATATGGGCTTCCTGCAGACCATACTCCAACCGATACAGAAGGTACAGGGA CTGCTTGTCAATGCGGAAAATTTCGAGAAACAGGCAGAAAAGGCTACATCACAG GCCAAATCATCCAAGGCTCCAACAATACCGGCCGAATCAAAGGAAGCCAGGGA AAAACGGGAAAAGATGGAAAAGCTCCTCAAGAAGGCTGATGAAGCAACCGCCG CAAAAAGGTACTCCGAAGCAATGACATGGCTGAAACAGGCACGGGTACTGGCT CCTACAGAAAAACAGAAGGATATTGACGAAAAGATGCAGGAAGTACAGAAACAG GCTAGTGCAGGAAGCCTGTTCGGTATGGCAGAGGAACCGGCGCCGGTAATTCC CCAACCACAAGGCTATATGAACGGTCAGTCACAACCAGGTATGCAAACAAGCAT ATTCCCGGAGCAACAGACCCATACTATGAATCCTGAACCTGTCATGCAGCCTGC TCCACAGCAGGTATCACAACAAATTCCACAAGGAATACCTCAACCGGCATATGG AACGAACGGGACATATAACCCACCTGCTCCAAACAGCCCGATAGTAAAAGGAGC AGACATACCGCAAGGCGCAACAATGCATCCTTACCCACAGCAGCCATACTACCA GCAAGAGGCGACTCCTTATCCAACACAACAGCCACAGCAACCGACAAACGGAC ATATACCGAATGGGGCTGCGCAAGTACAGAATGGAAACGGACGGGAATACCAG ACTGCATCGGCTACACATGAGACATTCTGCTTCGATCCGGAAGACGAGAATGAC AGGGAACTTCTAAGAGAGGACCCGTATGCGGAATATCCGGATTTTCCGGCTGA GTACCGAATGAAGGACGAGGCACAGGTAGAAATGGTATACTGCTGATATACACA ATAAACGATTTGTAAAACCAATAAACTATAAACAATATGGCACTGGAAATTAAAG GAATGAAAAGAGTATTCAAGATGAAGAAGAACAATCAGGAAATCGTACTGGATG ATCCGAACGTAAACATGTCTCCGGCTGAAGTGATGGACTTCTATTCCATGAATTA TCCGGAACTGACAACCGCGACCGTACACGGACCGGAAATCGAAGACGACCGG GCGGTATATGAATTCAAGACCACTATCGGAGTAAAAGGGTAAGAGCATGAAAAA AGGACAACGTAAAGACAAGAAACCATGTACACAACTTACGGAACGGGCTTTGGA AAATTTAGCCAGACTTATCATATCGGAACTCGAAAATACGGACATAAGCCGGGG CATCAGGAACAGAAAGAAAAGAAGACTCCCTCCCGCAGAAAGCCTCATGGTTTT CTGAACACGAGAATACCTTCCATCGCTCCCGATCTGTATGTTGAGAATGACAGG GATGTAACGGTAAATGTCACCACCAAAGAGAATCTTGATTTCCTGTACCGTTCAG CCATGAAGTATGCGCAGCTCCTGGATGTGGAGCTGCCATACCATCCTACAGGC AGGACTTCCACAAGAGAGAAAATATGCCTGCTATATAATGCACTGGATTCCATAG TATCTCATCATGTAAATCTGGAACTTATTGGTGACAGGCTCCAGTTCTGCATCTA CCATTTCCATGAATGGCCGGATTATACGCTTTTCTTTATGCCGATAGACTTTACG GAAAGGCTGCACGGTGAAATTAAAAAGATTACACTGGAGTTCATCAGAAAGTTC ATCAAATATCACAGGATGATGGATATAACCGATACCCCTTATTTTGAGATGTCGG AAGTCTGTATCGATTATGTGGACTTTGAACAGCTCGATGAGGAAGAGAAAAAGG ATTTGTACAGAAAGGAAAAGCTTTTCAGGTCATATGAGAAAGGGAGAATCCACA GGAAGCTGTGCCGGATGCACTCCAGGGCTTTCTGTAGGAATCTGGAAGAACAT ATCCGCAACTGTACTCCTTCCAGCGATAAGGAAAGAAGACTTTTGGAACTGATTA CCGAAGGGCTGTCCCTGATTGCAAAGGACAGCCCTTATATCTTGAATTATGATTA TGATTTTGCAAGCGAAAAGGAACGGGATTTCGAGCCGCCACCGCTCGAATATCA GATTCTGCTTACATATTCCATCACGGATACGGTTACCAAAGACATGGAAAGCTGT TTCAGTACTGACTGTCAGGAAACATATAACCAGACTCCCGTATCATTTACCTTCA TCACGCCGGAAACAGAGGAACTTTTCAAGCCGGACAACTATCCGGAACGGTTTG AGAAATGGTTTGAGAAATTTGTAGAACATGTTACCTATAATTTATAAACATCATGA ATGAACTGACCAAAAATATGCAAAAAATGATGGTACCGAAGGCTGCAATCATAG CCTACAAGTATGAAGACAGAAGAAATCTTGATACCAGGTACTTTATAGAATTACG TCCAATCAGAAAAAGCGGACAGATGGGGGCAGGTATCCCCGTCACATACGAATT CATGAATACCCTGCTGGAATCCTATACGGAAGAAATGAGCGGGATACCGGCAG GCAGAGTCCCTGAAAACATGCTGGCCTGCAATCCGAGAAAAGGACAGGAAGAA TATATCTGGTACAATCCGCCCGGAAAAAGACAGATGTTCTTTCACAAGGATCTCA ATATACAGGACGGCATGTTCAATCTGCCGGGAATTATCTACCAAGTAAAAAACG GAAACATGGACGTGTTCGCTTTCAAGGGGAAACGTCCGGTGGAGACGACTCCG CTGTTCCGTGCCCCGTTCTTCAACGTGACCGGATCAAGTGTCTGCCTTGGCAAC AGTTCTCTGGAAAAGCCACAGAACCCGACTTTCCTTTCCCTGCTGGAATACTGG GAAAAACGGTTCTGGCTGACTGAATTCTCCCATCTGGGAGGAAATGTCAATCCT ACCGTTTCAAATCTTGTCATCGTCACCGAAAATATAAGAAACAATCCGTTCGACA TGAACGAACTCAAGCCCATGAATAAAAAACTTAAAGACATACTTCCATGAAAAAG ATACATTTTACCGACCGCTACCTGCTCAATCCACGTCATCCGGTAACGGTATTC GTCATCGGAGCTGGAGGTACCGGCTCACAAGTGATAACCAATCTGGCACGCAT GAGCATGGCACTTCAGGCATTAGGTCATCCGGGACTGCATGTCACCGTATTCGA TCCCGATACGGTTAGCCAGGCCAATATAGGACGCCAGCTTTTCAGTGAGACGG AACTGGGACTGAACAAGGCCGTATCACTTGTCACACGCATCAACCGTTTCTTCG GATACGCATGGACTGCCGAACCGAAATGTTTCCCAACGAAGAAATTTTCAGGAT ATGATACAGCCAACATATTTATCACCTGCACTGACAATATACGTTCACGTCTTGA GATTTGGAAATTTCTAAAGAAAACTCGTAAAGAGAACTTCAATGACTATTTGGTT CCTATATATTGGATGGATTTTGGGAACAGCCAGACAAAGGGACAGGTCATCATC GGGACGGTACGTGAGAAAGTTCTCCAACCTTCTTCACAAGAATATATTCCCATG CCTAAAATGAATGTCATCACCGAGGAAGTGGACTATGCGAAAATCAAGGAAAAA GAATCAGGACCAAGCTGTTCTCTGGCGGAAGCCCTGGAAAAACAGGATTTGTTC ATTAACTCCACACTGGCACATATCGGATGTGACATATTATGGAGAATGTTCAAGG AAGGAAAGACACTGTATCGCGGTGCCTATGTCAATCTGGATACATTGAAAATGA CCGCAATCCCGGTGTAATGACAGAAGTGACCGTATCATCTTTCCATCAGAATAC GGTCACTTATTCTATTTGCTACTTATTATTTACTACGTTCTTACCACGCTGGAGCA GGAAACTCTGTATCTCTGAGGCGAGATAGAATGATTTCCCGTTCTTTTCCACCGA GTAATATTTAATCTTGCCCTCTTGCCTGTAACGTGCCAAAGTTCTTTGTGACACA CCAAGGAGTTCTGCCAGATCCACATTATCAAGCAGTCTGTCTCCATTCATACATT CTTTCAGACGATTCATCTGGTCCAGTTTCTTTTCAATGCGGGCAAATCCCTCTAC CATTGTTCCTATAAGTCTTTCGAGTATCTCATTATCTATATATGACATAATTCCAA TGTTATTAAGTGAATAAATCGATACTCTCTTCGTGCGCACTCTAAGAGTATGTAC TTATAGTAGTGAAAATAGTATGCCTGAATCTAAGACAAAGATCAACAAGCTTATT AGGCGCTGATAATCAGGCGTATAATTTTTTCTACTTAATATTTAGTGTAAACCAAA AGTGTAAACTATGTAATACAGAATTGGGAACGGGTTAACACAGCCACCAACAAT GACATCTGATGCTACCTGACGACACCTAATGACAACATTTTGTATCATATACATA TTCAAAATACATTTGTACAAACTCAACTTTTTTGGATATGGAAATCATTGGAATTG AAACAGCTACATATGAAAAGACATTAAAGGAAATTGAAAACTTCCTTGATACCAT TGATAAATTGATTACAGCTTCTTCACAGAAAACAATAGGGGAATGGTTGGATAAC CAAGAAGTTTGCCTGATCCTCAAAATTTCTCCAAGAACATTACAGAATCTTAGAG ATACAGACCAAATCTCTTATTCTCAAATTGGGAAAAAGATTTATTATAAAAAAGAA GATATTCAGAAGTTCATTGAAAAACACAACAGAAAATTATGAGCAAGGTAATTAC CCAAGATAATGAGCAAGTTATTCAGATATACAATAGGTTAAAAGATACGCTAACA AGACTCGAAGATATTCTGAAGAATAACAACCCAACACTTAATGGGCATAGATATA TGAATGATGCAGAATTGGCTAATTACCTTAAAGTATCAAGACGCACTTTACAAGA ATATAGAAATAATGGAATCTTATCTTATTATCAGATTGGAGGTAAAATTCTATATC GGGAATCTGATATAGAAGAACTTCTTGAGAAAAACAGACAGGAAGCATTCCGTT AAACATTTCTTGGAATTTTCGTTGATTTTCAAAGCAAAAATCAGTATCTTTGCAAT ACTGACAAAGAGTTGTATATCAGTGCAGAACAAAGAAGTTCAATCGAGGTGAAA TAGGTGGACTAAATGACAAACAACAAGATAAGTAATTGATTATTAGCGATAAAAA ATATAAGGTTCCGCCCCCAGGCGGATCACTGAAAACAAAAGAGAAAT
[0229] SEQ ID NO: 73 (plasmídeo pWD035 com PUL porfirano): aacaaatactttcaggacgatgtaaagtcctgagaaatcaaactgaatggatagataaatattagtcttttct catcggattcttttaatatcgctactcttttattatgtagtggttgtattatcttaaataaaacaaaaacacccgactttacattct tgggtgtaaaacagggtgtttgttttgcaatctgctgattttcagcgttttttgcggagagacaggctctcgaacttatactttc acagaatatcataaaactgcaaacgactgataatcaagtgtaaattacaatatagagtaaaagtaaatgtttctaaatg tcttttgctatctcaataattgggtgtatatttgggtgtagaatttcaaacgcacccaattatgaatatcaagcgcaacatca tttttgcattggagagccggaaaaagaacggtgtgccaatcgtagagaacgtacccatccgtatgcgtgtcatctttgcc agccaacgtatcgagtttacaacgggctaccggattgacgtagccaaatgggatgcagataagcagcgggtaaag aacggatgtaccaacaagctaaagcaaagtgcagccgaaatcaatacggacttgctgaaatactatgccgaaatcc agaatattttcaaggaatttgaggtgcaggaggtcatgccaacgacccaacagttgaaggaagctttcaacatgaga atgaaagataccagtgaagaacagccggaagaagcccctgtcagcttttgggaggtgttcgatgagtttgtaaaaga gtgcggtaaccagaataactggacggcatccacctatgaaaaatttgcagcagtgaggaaccacctcaaagagttc aaggaggatgcaacgttcaactatttcaacgagtttggattgaacgaatacgtcaacttcctgcgtgacaccaaggata tgagaaacagcaccatcggcaagcaaatgggattcctcaaatggttcctgcgctggagcttcaagaaaggacatcat cagaacattgcatacgatacgttcaaaccgaaactgaaaaccacctcgaaaaaagtaatcttcctgacttgggatga actgaacaagctgaaagactaccagatacccaaggataagcaatacctggaacgtgtgcgtgatgttttcctgttctgc tgctttacgagtttgcggtattcggatgttcgcaatctgaaaagaagcgatgtgaagtccgaccacatcgaaataacca cagtcaagactgccgacagcctgacgattgaactgaacaaatacagcaaagccatactggacaaatacaaggac atccatttcgagaattacatggctctgcccgtcatcagcaaccagaagatgaacgattacctgaaagagctgggcga actggcagaaatcaacgagcctgtacgggaaacctactacaagggaaatgaacgtattgatgaagtcacacccaa atacgctttgctcagtacccatgcaggaaaaaggacattcatctgcaatgcgctggctctcggaatcccggcacaggt ggtcatgaaatggacgggacacagcgactacaaagctatgaaaccctacattgacatagcggatgatattaaggca aatgccatgaacaagtttaatcaactataaaagtatcaaacagtttcatagatagttaccaatgactgataggtgggctg cccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcaga gcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcc tacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgta tatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaac ggaattgatccggccacgatgcgtccggcgtagaggatctgaagatcagcgggattaaaagtcggggattggtgaa caaaaaggtgtttctctctttaagagaaatatcgttttgctaaacagttgatattgaggtatcattttatcgtaaaagacatttt tgctcaacaattgcttgacggaaatcaacaaattttagcattttgtaaaaaagtcgctatataatttggtgaattggagttat tttcatatttttgcatcccgaagagtttctcttaaagagagaaacatcttttgcataccttttccgaccgaatttttatgtcgtaa agaggggctttgcagggggtggactcagaaagatgagaatagatgactattgtagttgaaacacatagaaagttgct gatatacagaccgatacgcatatcgggatgaaccatgagtacgttcttttctcaaaaaacataaatattcgaaaagag atgcaataaattaaggagaggttataatgaacaaagtaaatataaaagatagtcaaaattttattacttcaaaatatcac atagaaaaaataatgaattgcataagtttagatgaaaaagataacatctttgaaataggtgcagggaaaggtcatttta ctgctggattggtaaagagatgtaattttgtaacggcgatagaaattgattctaaattatgtgaggtaactcgtaataagct cttaaattatcctaactatcaaatagtaaatgatgatatactgaaatttacatttcctagccacaatccatataaaatatttg gcagcataccttacaacataagcacaaatataattcgaaaaattgtttttgaaagttcagccacaataagttatttaatag tggaatatggttttgctaaaatgttattagatacaaacagatcactagcattgctgttaatggcagaggtagatatttctata ttagcaaaaattcctaggtattatttccatccaaaacctaaagtggatagcacattaattgtattaaaaagaaagccagc aaaaatggcatttaaagagagaaaaaaatatgaaacttttgtaatgaaatgggttaacaaagagtacgaaaaactgt ttacaaaaaatcaatttaataaagctttaaaacatgcgagaatatatgatataaacaatattagtttcgaacaatttgtatc gctatttaatagttataaaatatttaacggctaaaaacaataggccacatgcaactgtaaatgtttacgcgggtaccgac accgcggtggaggggaattatcacgtgctataaaaataattataatttaaattttttaatataaatatataaattaaaaata gaaagtaaaaaaagaaattaaagaaaaaatagtttttgttttccgaagatgtaaaagactctagggggatcgccaac aaatactaccttttatcttgctcttcctgctctcaggtattaatgccgaattgtttcatcttgtctgtgtagaagaccacacacg aaaatcctgtgattttacattttacttatcgttaatcgaatgtatatctatttaatctgcttttcttgtctaataaatatatatgtaaa gtacgctttttgttgaaattttttaaacctttgtttatttttttttcttcattccgtaactcttctaccttctttatttactttctaaaatccaa atacaaaacataaaaataaataaacacagagtaaattcccaaattattccatcattaaaagatacgaggcgcgtgta agttacaggcaagcgatccgtcagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaata ccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccat gtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcg agcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggtta ggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataa acaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtata aatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtt tctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatg cctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagc attccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcg attcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctagctcaggcgcaatcacgaatgaataacggtttggtt gatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccatt ctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattga tgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcatta cagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttct aatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaa atgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgc gtcaatcgtatgtgaatgctggtcgctatactgccagaagagagaaagaaggaaagcggccgcacaggtttcccga ctggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatg cttccggctcgtatgttgtgtggaattgtgagcggacaacaatttcacacaggaaacagctatgaccatgattacgcca agctatttaggtgagactatagaatactcaagcttgcatgcgatacgtatcgttaacgatggatccgacgcacgtgcga attcgccctatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtcaccc aacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccc aacagttgcgcagctgaatggcgaatggcgcctgatgcggtattttctccttacggcggccgcttgacataacttcgtat agcatacattatacgaagttatgtttaaacattagcagaaagtcaaaggcctccggtcggaggcttttgactaaaacttc ccttggggttatcattgggtcgagaccgcctgaagaggacttccattgttcattccacggacaaaaacagagaaagga aacgacagaggccaaaaagctcgctttcagcacctgtcgtttcctttcttttcagagggtattttaaataaaaacattaagt tatgacgaagaagaacggaaacgccttaaaccggaaaattttcataaatagcgaaaacccgcgaggtcgccgccc cgtaacctgtcggatcaccggaaaggacccgtaaagtgataatgattatcatctacatatcacaacgtgcgtggaggc catcaaaccacgtcaaataatcaattatgacgcaggtatcgtattaattgatctgcatcaacttaacgtaaaagcaactt cagacaatacaaatcagcgacactgaatacggggcaacctcatgtcgcctgaagagtgagaccgtcccaactttca ccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggaga aaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctc aatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttat ccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtg atatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacg acgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtt tattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaactt cttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatc atgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggc gtaaaaatgtaatcacctggctcaccttcgggtgggcctttcacacttgcatcggatgcagcccggtgaacgtgccggc acggcctgggtaaccaggtattttgtccacataaccgtgcgcaaaatgttgtggataagcaggacacagcagcaatc cacagcaggcatacaaccgcacaccgaggttactccgttctacaggttacgacgacatgtcaatacttgcccttgaca ggcattgatggaatcgtagtctcacgctgatagtctgatcgacaatacaagtgggaccgtggtcccagaccgataatc agaccgacaacacgagtgggatcgtggtcccagactaataatcagaccgacgatacgagtgggaccgtggtccca gactaataatcagaccgacgatacgagtgggaccgtggttccagactaataatcagaccgacgatacgagtgggac cgtggtcccagactaataatcagaccgacgatacgagtgggaccatggtcccagactaataatcagaccgacgata cgagtgggaccgtggtcccagtctgattatcagaccgacgatacgagtggtaccgtggtcccagactaataatcaga ccgacgatacgagtgggaccgtggtcccagactaataatcagaccgacgatacgagtgggaccgtggtcccagtct gattatcagaccgacgatacaagtggaacagtgggcccagagagaatattcaggccagttatgctttctggcctgtaa caaaggacattaagtaaagacagataaacgtagactaaaacgtggtcgcatcagggtgctggcttttcaagttcctta agaatggcctcaattttctctatacactcagttggaacacgagacctgtccaggttaagcaccattttatcgcccttataca atactgtcgctccaggagcaaactgatgtcgtgagcttaaactagttcttgatgcagatgacgttttaagcacagaagtt aaaagagtgataacttcttcaacttcaaatatcaccccagcttttttctgctcatgaaggttagatgcctgctgcttaagtaa ttcctctttatctgtaaaggctttttgaagtgcatcacctgaccgggcagatagttcaccggggtgagaaaaaagagcga caactgatttaggcaatttggcggtgttgatacagcgggtaataatcttacgtgaaatattttccgcatcagccagcgcag aaatatttccagcaaattcattctgcaatcggcttgcataacgctgaccacgttcataagcacttgttgggcgataatcgtt acccaatctggataatgcagccatctgctcatcatccagctcgccaaccagaacacgataatcactttcggtaagtgc agcagctttacgacggcgactcccatcggcaatttctatgacaccagatactcttcgaccgaacgccggtgtctgttga ccagtcagtagaaaagaagggatgagatcatccagtgcgtcctcagtaagcagctcctggtcacgttcattacctgac catacccgagaggtcttctcaacactatcaccccggagcacttcaagagtaaacttcacatcccgaccacatacagg caaagtaatggcattaccgcgagccattactcctacgcgcgcaattaacgaatccaccatcggggcagctggtgtcg ataacgaagtatcttcaaccggttgagtattgagcgtatgttttggaataacaggcgcacgcttcattatctaatctcccag cgtggtttaatcagacgatcgaaaatttcattgcagacaggttcccaaatagaaagagcatttctccaggcaccagttg aagagcgttgatcaatggcctgttcaaaaacagttctcatccggatctgacctttaccaacttcatccgtttcacgtacaa cattttttagaaccatgcttccccaggcatcccgaatttgctcctccatccacggggactgagagccattactattgctgta tttggtaagcaaaatacgtacatcaggctcgaaccctttaagatcaacgttcttgagcagatcacgaagcatatcgaaa aactgcagtgcggaggtgtagtcaaacaactcagcaggcgtgggaacaatcagcacatcagcagcacatacgac attaatcgtgccgatacccaggttaggcgcgctgtcaataactatgacatcatagtcatgagcaacagtttcaatggcc agtcggagcatcaggtgtggatcggtgggcagtttaccttcatcaaatttgcccattaactcagtttcaatacggtgcaga gccagacaggaaggaataatgtcaagccccggccagcaagtgggctttattgcataagtgacatcgtccttttcccca agatagaaaggcaggagagtgtcttctgcatgaatatgaagatctggtacccatccgtgatacattgaggctgttccct gggggtcgttaccttccacgagcaaaacacgtagccccttcagagccagatcctgagcaagatgaacagaaactga ggttttgtaaacgccacctttatgggcagcaaccccgatcaccggtggaaatacctcttcagcacgtcgcaatcgcgta ccaaacacatcacgcatatgattaatttgttcaattgtataaccaacacgttgctcaacccgtcctcgaatttccatatccg ggtgcggtagtcgccctgctttctcggcatctctgatagcctgagaagaaaccccaactaaatccgctgcttcacctattc tccagcgccgggttattttcctcgcttccgggctgtcatcattaaactgtgcaatggcgatagccttcgtcatttcatgacca gcgtttatgcactggttaagtgtttccatgagtttcattctgaacatcctttaatcattgctttgcgtttttttattaaatcttgcaatt tactgcaaagcaacgacaaaatcgcaaagtcatcaaaaaaccgcaaagttgtttaaaataagagcaacactacaa aaggagataagaagagcacatacctcagtcacttattatcactagcgctcgccgcagccgtgtaatcgagcatagcg agcgaactggcgaggaagcaaagaagaactgttctgtcagatagctcttacgctcagcgcaagaagaaatatccac cgtgggaaaaactccaggtagaggtacacacgcggatagccaattcagagtaataaactgtgataatcaaccctcat caatgatgacgaactaacccccgatatcaagtcacatgacgaagggaaagagaaggaaatcaactgtgacaaact gccctcaaatttggcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggctgaaggaaacagcaa aactgtgacaaattgccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacagataaccctcag actatcctgtcgtcatggaagtgatatcgcggaaggaaaatacgatatgagtcgtctggcggcctttctttttctcaatgtat gagaggcgcattggagttctgctgttgatctcattaacacagacctgcaggaagcggcggcggaagtcaggcatacg ctggtaactttgaggcagctggtaacgctctatgatccagtcgattttcagagagacgatgcctgagccatccggcttac gatactgacacagggattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaagccgaaactgcgtaa accggttctgtaacccgataaagaagggaatgagatatgggttgatatgtacactgtaaagccctctggatggactgtg cgcacgtttgataaaccaaggaaaagattcatagcctttttcatcgccggcatcctcttcagggcgataaaaaaccactt ccttccccgcgaaactcttcaatgcctgccgtatatccttactggcttccgcagaggtcaatccgaatatttcagcatattta gcaacatggatctcgcagataccgtcatgttcctgtagggtgccatcagattttctgatctggtcaacgaacagatacag catacgtttttgatcccgggagagactatatgccgcctcagtgaggtcgtttgactggacgattcgcgggctatttttacgtt tcttgtgattgataaccgctgtttccgccatgacagatccatgtgaagtgtgacaagtttttagattgtcacactaaataaa aaagagtcaataagcagggataactttgtgaaaaaacagcttcttctgagggcaatttgccacagggttaagggcaat ttgtcacagacaggactgtcatttgagggtgatttgtcacactgaaagggcaatttgtcacaacaccttctctagaacca gcatggataaaggccaacaaggcgctctaaaaaagaagatctaaaaactataaaaaaaaaataattataaaaata tccccgtggataagtggataaccccaagggaagttttttcaggcatcgtgtgtaagcagaatatataagtgctgttccctg gtgcttcctcgctcactcgaccgggagggttcgagaaggggggtaccccccttcggcgtgcgcggtcacgcgcacag ggcgcagccctggttaaaaacaaggtttataaatattggtttaaaagcaggttaaaagacaggttagcggtggccgaa aaacgggcggaaacccttgcaaatgctggattttctgcctgtggacagcccctcaaatgtcaataggtgcgcccctcat ctgtcagcactctgcccctcaagtgtcaaggatcgcgcccctcatctgtcagtagtcgcgcccctcaagtgtcaatacc gcagggcacttatccccaggcttgtccacatcatctgtgggaaactcgcgtaaaatcaggcgttttcgccgatttgcgag gctggccagctccacgtcgccggccgaaatcgagcctgcccctcatctgtcaacgccgcgccgggtgagtcggccc ctcaagtgtcaacgtccgcccctcatctgtcagtgagggccaagttttccgcgaggtatccacaacgccggcggtcgg ccgcggtgtctcgcacacggcttcgacggcgtttctggcgcgtttgcagggccatagacggccgccagcccagcggc gagggcaatcagcccggtgagcgtcggaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgt gaaggtgagccagtgagttgattgctacgtaaataacttcgtatagcatacattatacgaagttatggactacgcaggtc aatatccggaacatgaaacccgcaggttctgaaatccgcatattcagaacatggggattcgggaagcgcagcaata cgggcagtctcaggttcgatgctccggtcaggaaatgggaatacagagaaccgaatccgttatatgacggttacacc acccgtaactggttccgctatcatatcatgaaacaccgggacagggaaaggacaggcgaatacaccttccgcagcg attcattcacgctgtacagccggagcgagctggacgagctggccgcaatcctgaaaggcagactctacaagggaat cctgcctgactctcttgtactttggggataccgcatggatattaaggaaatatcacgtgaacagtggaacggtatgggac agcacggacaaatccgcatgaaattcatgggatacggtccggtcagaatccacacggacaatgaaaaccataccg taacagtatacagaatcaacgacatattgtcttcaactatcagaattttcatattttttcagttctttttttgtttcttctattaatattt taagccactccatgatttgtattgcatgttcatgaacagtttcattttggctatcactgtcgtgtagtagcctttgaaaatcacg taaaatattgtctttcccaagcatctcccatacaggcatcatccggtggattatttttctcatggtctcacggtcggttatcctg tcagcagattccatctcctccagttctttttcagattccataacaacgagagaaagcatatgactataatcatccgtattctc cagtaaactggaaaaatcgaattctccggaaactgaaacttgtgtacgagatataatggtggataaaaaagcaagca gtccgtggatattgaacggtttatgaatacagcctacaaatccttctttttcataaattccggaatttccgtcaccacgggc agtcatgactgctactggaacagttctagaattgccgatgtccgaattgcgaagcaatcttaacaaaccgaatccgtca gtatcaggcatttgtacatctgtcaagatcaaatcatattcagaattttcaagagcggccactacttcacgtgcattcttac aggttttacaggatatacctttgcgcccgagcatatcttccgctattttcagttgtataggatcatcgtccactacaagaac attcttaggcaatatagttattgtattatggtccgatttgtcttcctcaactaactcatccgtttcaggcaaagaaagttccagt ctgaacatgcttcctttaccgagtacactttctacatccatttttccttccaaaaccttaattaatcctttggtaaggaaaagtc ccaaaccaaacccttcagaattgacattctgtgcggcacgctcaaatggagcaaatattcttttcagtgtttcctcatccat accgataccagtatcccttatttcaatacgaagttttccttctgaatattctgaatggaaattgacgttacccctggaagtaa acttaatagcgtttgtaagtagattggctaaaacctgttcaagtttgtccgcatcaccttttactattacatttgatcctttatgtt cagaatataaaatcagaccttttgaagtcgctttacgagaaaactcatctgaaattcgttgcaagaaacggtcaagata aaatggtgtgtcgttacgcaaattaccggcttcattgattcggtaagcatccatcaaatcattaaccagatgtaaaacgtg tcgacaagaatgacggatgtcatctaaatatttttcgcgcttcctcttttcacgcgtttcagataccaaatctgcacagttat ggatattaccaagtggacctctaatatcatgagaaactgtcaggatgattttcttacgcatatcaagcaaattctcgttttctt gaatagcttgttgtaatttaaatttaattatttcttccttacgtaaatctgattgtataattaaaaatgaaattaatattataaaaa ccgcaatactcatcattacgataaataatcgaaaggattcttgtttgacttccgttacctctaagtttcgttctataaatgaca gctgtacctgattatctaaaaaagatacaaaatcatataatttttgatttaacagcctattctgcaaacgcaagctatccac ataagtttctatctgattgtttcgcatatctatgacagaaaccaatctattattaaaattctgtatttcattagttatatagggga cttgtatcgtctccttctttccgaataatccggcaattcctttctttttctgagttattgtcttcacttttactgtttgagtagctattac aggcaattcattagtaagaatactatcagatttattcgcaaattggactgctttcattatttgaaacaagtgcatttctttcgttt taagcaattcccgtaaagaatcaatttgaactggacataaaaaatcacaactccttaattttatttcaagtagaacactat ctgttttaaaacgttgattatgaaatatgttataatcagactcatcccatactataactgattcgcctaaagttgccaacttag taatatacaaatgaactttattagtattctcataagcttcattaatttgaattatcagattctcaagttctttcaaccggcaacg ttcatttatcattacagtaaccatacttaagactataaatcctgtaataaaatatccaataaatagtcttttgcgtaataatga agtcatcaggaacattctattgatttatttgacatcataattctatatatttaactagtcatagtatatatcattctcaaatatttat ttcaaattcaagcaataaaataaaaaaacacttcatattacaactgaactcttttatgaaaaagttgaatatatgaagtgt ttttttattacgatataaactataaaatcctattcttcgggaactggtgtataaacccttatccagtccaccaggaaggtgtg gtcttccacatttttcagttcctcatccgtagggcttaaacctttaacggctctccagctttggtcttccatatttattatgatgtc catgtcttttaccagacctgtaccaccagtgtagttgttggggtcgataatatccttgccgcttacggttctgacaagttctcc atctacataatattcaagtgtgaaagggtctttccagaacactcctacacgatgaaaatcgtcgcgccacaatgttccctt gtcatccttataccatgagccaagatctttcggctgataatccttgaatggctggcggatgaatatgtgatggctcaggtg aagtctgtcggcaccgtaacctccgccgtctctgtcgccgccgtatgcttctatgatgtcgatttcctgagtatcgtcaggg ctgagcatccatacatcggatgccatggttgaatttgaaagttttgcgtatgcctctacataaaccggatactttacacgtg tcttcgatgtgatacatcccgtataggttcccggcagttcctttgtgttgggtccgcttacaactttcttcatggggacatcttc aggacggctggctcttattttaaggtatccgtcggaaacggaaacatggtctctctgccatattgtaggagcaggtcctgt ccaatgattatgatagaaatcggtccatttggcatagaactcttttcctttatccttttcgtcggcaacataattaaagtcgtc cgactgtggatggagtttccacaccataccgtcgccggcatcagcgggtacaggatagatatcccactcgtacgattta ttattgaaatcttctgctgcacaggctatttgcagcgatgctaaacaaatggtaaacagttttctcatcgtggtatcttagttt aagttataataattattttcgttcttttgattcacctttagcggtatgtgtctgcaatgtccaggtagaaaatctcattatgctctg atagtctgaactgttgtatatatgagtaagaccccatctcaatatttcggtaggttctttttcggcatctgcactgcggttcag gccaatggcgtgtggcgcgccttttactactgacattatttcaaagtttattccgtcaggcgaccactggagtgtgttcttttc aggaccgtcggtggtgataagtgaagctatacctcctttgtaaggccatacgcaaacttcatgcccgctgtttgaaatag gattatattccgatttcacatacggacccataggattttccgcaatagccactccgtgtttgatttcacggccgccccatgtt atttcttctcccatacgttcgcctttgtagtacatatagaacttacctttataaggtattatacacgggtcgtgtaccttatgact gtcgaaatcacctttcgacactaccttgaatctgttatcctcatcgccttcccattcgccggtattagaaggttccagtaca ggcttgtctgtcttgatccacggtccttcaggggaatcagcacatgccataccgatagtattctttacacggactgtgtaa ggggattttaccgcctgatagcaaagataatactttcctttccattccatcacctcaggagtgaagactgaacggtcgtc gtaagcacctttttcaccacgtttcactgcaattccctgttccttccatgtccatccgtcttttgatgtggcataccatatatcac atctgtcccatgggaaaaccttatctttctctatatctccagcaaatccttgggtaggtccatagctctttgaataccatacat aatatgtattacctattttcagcattgcactcgggtctcttcttactacgccctcttcataagcaagatcacctttaagtggttc catcttatactcaaagaaccatttattgtcgtgattttcccatttcatggcacgtttcatagctgcacttaacttatttcccttagg tattcccaatgaatcggccttacgctcatcataattctgagtgtcgtcaacggcaatagtctgtgtattgcctgtatttccgca tgctgccaatagcgacatcatgccggctgcaagaataatttttctcatactagactttattttatattaattgttagtttattcga gtgtaattcacttgtttctgcactgatattcagtaccgatgatttttctgtcgactgaagcatcagcatacatcttccctgatat gtcataatatccttactttgatatggagaaacgttcttcacgtttccattgtctataccaagcagacggtactctccatcaat gttgaacttaagcatctgttctgttgtctttacaggattaccttttttgtctgttagctgagctgtgacatgcagaacatcctttcc atttgctgcgatactttgtttgtcaaccgtcagcaatatcgaatgttctttgcctgaagtccttatagctgtagtggtattaccta acttattttttccttttgcggtaatagtgccaggcttgtactgaactgcccatttatagatatgatcctcaaaatcgtctatatac ttctttcccatcgacttaccgttaacgaaaagttccacttcatcacaattggaatatatctctactattaccgagtcacctttct gataattccagtgagagtttacatcatcccaaacccataattttctatcccattcatgtcctttcttatcagtaaatccatctttt acatggagatacgaagatttgtctgtagtctgtgaatatatagcaataaaaggcttgtctgtccacaatgatttcatcatgt cgtacgaaggcttcacatagccgcacatatccaggagaccacatcctatcgacttttgaggccattttgaaagacggct ttcactttctcccagataatcgactcctgtccatataaacatacccggaacgaaatccctttcaatcaccgccttccattcg tgccactgaccgagattttctgtacccattataggcttgtcaggataattcttcttagcataatcatacatcacgcgacggt agctgaagcctgccacatcgagcgcgtcgatatatcctgactcaaagcttatggaaggcaggatgcagttggcggta actacacgtgtggtgtccatctggcgtgtccatgcagctaatttttgcgctgtacggccaatgtcgtatgcatgtttaggctg gattttccacatttctctgattttttctttagagtatggaggctgattccagaaataattaccgttggaatcggcaccgaaga aacctgtcgcctcgcggcatccggtataagtccattctatttcattacctatactccactggaagatacaggcatgattac ggcttctcctcattacgtttttcaaatctctttctgcccattcctggaaatgctcgcaatagccatgcgtaggatagtcttctac agtttccttcatattgagtcttttatctttgggataatcccactcatcgaagaattcttcctgaaccagaagacctatctcatcg cacaaagacagaaactcttccgctcccggattgtgcgagaggcggatggcattgcatcctccttcctttagggttttcag acgccggtaccacacatcgcgtatcattgccgcgccaaccattccggcatcatggtgcaggcatactccttttatcttcat gtttttcccgttaaggaagaaacctttgtctgcatcaaaacggaatgtccgtatgccgaacctgacagtgttttcagaaatt acttcatcgccattcttgatgcgtgtctcggctgtatagaggacaggtgtatcgacgctccacaaatcaggctgtttaatct cagatacgatgtcgataattttctcctcaccagcattcagttttatactgaagacctcaaaggctgcgatattgcctttattat ccttatatactacctcaacaactgcagctctgggttcggagtagctgttgcacacggtaacctggttgtttactttagcatat ttatcagtaaccacgggagtagtgacaaatgttccccaaaccggaatatgcagtctgtcggttacaatcattttcacatc cctgtatatacctgaaccggtgtaccatctgctgtcggcataatggctgtggtcgacccttacagtcatacggttatcctca ttgggattgagatagtctgtgacatcaaaataaaaaggagcatatcccgaaggatgatatccaagctttttgccatttatc caatactcagaattattatatactccatcgaacactatatagcatttctgatttgcactgattgttgtgggaaatgatttgctat accatcctattcctccctgaaggaaagctacacatccttcacccgaaatggaatcgtaaggtaaaccaacactccagt catgtggcaggttcactttcttccattcatcaccagggacataagaagtatatgaataatgagcagaatctttcagtacga atttccaatctttattgaaatcaacatttgaatcagatgctgaaacctttagggttgataataggattattaaagctaaaag atttttatttctcataatcttaggttttacatgttttttgatgtcacaaaactatatctttcacttataatatatgagggggatattaa tgtgatatagggtgggaaatcagaattttacatctgccctgtattccaccgtcacctacaaccttgacaaaggatgttcctt tcttccctcttatggttctcaggacaaacagacactttccgttatatgtccttacactattgtttatgacgttgatgttcaaatctt ctatcgaaggcgatccattgtcgagtccggcaagttcaagcttgtcgtcgaggattatcctcacatccgaaggtatatcg actactgtgtttccttctttatcttcaatggatacttctacatggataaggtcataaccgttgtcggtagctgttttgcggtcgca gttcagtgccagacggcacggcttgccgcttgtggacaaagtgtctttcgacaatattctgtcgccgtccttgcctaccgc aaggagtgttccttccttgtatgccaccttccacatcagtatattatgctccatgaaatcgctgcgtttctttgttcccaacgat ttgccgttcagaaacagttccacttctggggcgttggtatatacctgcaccagtatgtcctcgtccctgcggtacttccattt atcgcgtgtgtcgtaccactcccagcgtctgatccatcccgggcgtggagtgtaggtgaaacttccgtcagtatccatctt gaactcgctttccttttcaggtattgttacaatatgggttttcggtgtgtctttccacagacattcaaagaaatggccacgcg ctgtcttgttgcccacgaaatcgaagaaagaacagtctccaccccttgcaggccatgggccgttctcgccaagatagt cgaatcctgtccacacgaagatgcccgctatgtacttcttgtcggccacggctgtccattcaaagagctgaccaacattc tccgaaccgataataggctgatatggatatagcttatggtcgatttcataatatttgtctttatagttatatcccactacatca agaacgtctgtatatccggagagacgcgaaactgacggaacaacgactcctgaagagacgggacgggtagtgtcc acatccttaacccaaccggcaaggacagcggctgtttcagccaaatcgtcttttcctcctgacagacggttgaactctttc agtatagacttgttgtctgtttccgggtcgcccgtatggataagacccttgaaccctttattgtctttgctcgatgcccagtaa tatggataggtccattctatttcattgcctatactccagagtatcacgcaaggatgatttctgtctcgcctgatgaacgactt gaggtcgtgctcggcatgcgtatcgaagtatctggtatatcctattgatatgctgtcgggcgcatcttccttagctcgctca gtaatccactttttctttgccaccttccattcgtcgataaattcattcattacaagaagtcccagactgtcgcacatttccagc agactttccgaatgcggattatgggctgtacgtatggcattgcagcctatggaacgaagtttcagaaggcgtcgcaaca gggcatcatcgtatgcggcaacacccatacatcccaagtcgtggtgtatgttcactccttttatttttactgattttccgtttag aaggaagccttcatccgcatcgaatttaatgtcgcggataccaaattttgttgttttcttatccatcacatatccgtcagaag caatcagagtagtatgaagctcatacatcgaaggcgtttcaagactccagagatgacaattctccagttcaacagatg cagtgaactcattgaaatcgcctttcagggcaacaaaatcatcggaaacagaagctattgtcttgccgtcgtacactac ttcgtgcttcacggtgactccttttacacctgttccagcattcttcacctcgcataccacattcaccatcgaacggttgccta cctgtggtgtggtaacgaatattccgtctgaaggaatatagagctcgtttcttagaataagactcacattcctgtatatacc ggcaccgacataccatctgctatcggcatacgctcttctgtcaacgcagacagttattgtattcatcgaaccttttggtttca gatattgagtaagttcatattcaaatcccacatatccgttaggacggaatcccaacatatgcccgtttatccaaacctttg agttattatatacaccttcgaaatgaatgaacacttttttcccattcatatcatccgaggtgagaaaattcttcatgtaaatcc ccacaccgccagacagaaaaccattgcttccggctgtctgagtcttggtatatccttcgctgatactccagtcatgaggc agacacacatcctcccactttatatctggactcaggaacaaagtgtcctgaggcacgaaacctgctggtttgctgaattt ccaatcgaagttgaaatccactttagtggaggttccggcataacagaatccggacagaaagatagttaagactgtgat aatgttttttatggtcatatcgattttcagattaatattaatgacaaaaataatttcaaaagtgtaaaaacaaaaaaactctc catttatatttcagatatcaacggagagtttcatcattaaaaaaaataaaacattttataaagttactccttgcttaaggata gctatttcccggtatcccttcttttcgttcagtgcctgctttccgcttgccacttccaccacaaagtctataaaacgtctgctta aagattccatgctttctccctctaccagagttccggcattgaaatcaatccacgtatgtttctgttcataaagcggagtgttg gtcgaaaccttcacggttggaacgaatgttccgaacggtgttccgcggcctgttgtgaacagcacgatatggcatccg gcagaagcaagagccgtacttgccactaggtcgttgcctggtgcgctcaacaggttaagtccgtgtgttgtgacacggt cgccatatttcagaacatcctccaccatcgagcttcccgacttctgtgtacatcccaatgatttctcctcaagcgtggaaat acctcccgccttgtttcccggtgaaggattttcatatattggctggtcgttgcggatgaagtagttcttgaagtcgtttatcatg gccactgtgtcgtcgaatatctccttcgtgcggcaacggttcatgagcagtgtctcggctccgaacatttcaggtacctcc gtgaggactgttgtcccaccctgggcaacaagatagtcagagaacaccccaagcatcggattggccgtgataccgg acagtccatcagacccgccgcacttgagtcctatacgcagttttgacagggggacatcagtccgcttgtcttccctggct atggcatacatctcacggagaagtttcataccctcttctatctcatcatctactttctgagaaacaaggaaacggatccttt gggtatcatagtcacctataaactcacgaaaggcatcaggctggttgttctcacagccaagacctacgacaaggaca gctccggcattgggatgaaggaccatgtcacgcaatatcttacgggtgttctcatggtcgtcacccaactgcgagcatc cgtagttatgagggaaagatataatggagtcaaccccctcgcaacctgtttccttgcgaagctgctcggccaactggttt actattccgttcacgcaacccaccgtagggataatccatatctcattacgtatgccggcttctccgttagcacgcaaatac cctttgaatgtatggttctcgttcgtgaatgtctgtttctcgaacttcggagtgtaagtgtatgtactcagaccggaaaggttc gtcttgacggttttctcgttcagcagatgtcctttcctgacttcctttacagcgtgcgatatggggaaaccgtattttatcacca tatcaccttctgcaaaatccttcagggcaatcttatgaccggcaggtatatcctccattaattctatggaattgccgttcacc tctattacagtccctttggacaatgggtgcagtgccacagccacattgtccgcagggtttatctggatatattcagtcataa caaactaacatttataaattgaagaatacaggtagaagtatcaacctacaaggtcttttactgtctgaagcattccttcgct ctggattttgttgatatagtaaattacacggtctgccagtcccgagatagtattaaggtcttcaccccaaatggaagtatcg gcgagaactgtcttcacaagattttctaccgagccatcgttccacaaacttgtaagcatcgccatgatttcctgtgcatcgt taggaactatctctacaccatcggcacgctttccacctttgtagtatactatgatggctgcaagaccgagtacaagtcctt caggaagcacacccttacgtttcagatattccttcactcctggaaggtcgcgtgtggcatacttagggaatgagttaagc atgattgatgttacctgatggtctacgaaaggattattgaaacgttccaggacatcatcggcaaacttcttgagttcctcttt cggcaggttgagggtctccatcagctcgtcgaacatcacacgtttgatgaacttgcctatcacctcatgttggcatgcgtc tctcacgatattgacgcccgaaaggaatgccaccggcgacaatacagtgtgaggaccgttcagcagagtaaccttgc gttcatgataaggctcctccgacgggacgaacagaacgttcagtcccgccttgtttgcaggaaattcttcggcaaccga ttccggtgcttcgataacccacagatgaaaagcctcgccctgtacaactaaattgtcatcaaagtatagtttagtttttatgt tgtctatgtctttacgagggaaacccggtacgatacggtccaccagtgtggcatatacaccacatgcagtttcaaaccat gacttgaactcttcgccaaggttccacaattcaatatactgatagattgtttccttcagtttgtgaccgttgaggaagataag ctcgcatgggaagatgatgagtcctttcgacttgtcaccgttgaaatgtttgaatctgtgataaagcaactgtgtcagcttg cccggataagagcttgcaggagcatcctcaagcttgcacgacggatcgaagttgataccggcctcagtagtgttcga gattacgaatctcatatcaggctgttccgccagtgccatgaagtcattatactggctgtatggattcagcgcgcggctgat gacatcaatcattctgaatgagttcaccacctcgccattgttcagtccctgaagattgacatgatacagacagtcctggg cattgagggcatcaaccatacctttttctataggctgcaccacaacaacactgctgttgaaatctgtcttttcattcatattcg agataatccagtcgacaaacgcacgaaggaaattaccttcgccaaactgtatgatacgttccggacgtactgcctttac tgcagtcttactatttaaagctttcattgtaatgccaaaaaattaaaattgataagattaaaattcaaccaacattctgaata ccttacctggattttccgaccatttctgcagagcctcgcctgcctcttcaggtttcactacggcagagataagttcgttcatc gggcagttgccattctgaagataatgtatcacggcacggaaatcctcaggcattgcattgcgcgaaccgcgtatgtcg agttccttctggacaaaatattttgtctggaaagccacttcactcttggcatagccgatacatgccacacggcctgtgaaa cctacaatgtcgatggcagtaacatatgtgataggactacccacagcctctatcaccacatcagccatatagccgtca gtaagttcccttactctttccaccacattttcagtcttcgaattgataaccatcgaagcacccaggcgttttgccagttcaag cttctcatcgtcaatatccaatgctattacccttgcgccacgaagcgatgctcttactatggcgccaagtccaatcattccg caaccaatcacggccacagtatcaatgtcagttacctgagctctcgacacggcatggaaacctacgctcataggctc aatcagcgcacattccttatccgaaagaccggcagccggaataacctttgtccaagggaggacaaggaactcctgc atagaaccgttacgctgaacacccaaagtctcgttgtgttcgcaggcattcacacgtccgttgcggcatgaagcacact ttccgcagttggtatatggatttactgtcacgttcattcccttctcgaaaccgacaggaacgccttcgcctatttcctctatca cagcacccacttcatgtcccgggatgacaggcatcttcaccataggatttcttcccaggtaagtattaaggtcggaacc acagaatccgacatatttgatacgaagtaaaatttctccggctccaagtgttggtttaactatatcagctacttgaacctttc cggcttcagtaatttgtacagctttcataatctatgtatttatttaaatttgttattgtattattttgatgttgcattaattcaatgttgtt ttttctctatcttatatcctctccagccataatatgccgtaaagaagaaacatatcagaggtattacatatgccacctgata gaagtccgcgttatgattcatcacaaatgcggtgaactgagggatgcacgcattacctataatagccatcacaaggaa tgccgaaccactctttgtgtcctcgccaaggtcgcgtagtgcaagtgagaactgggttggatacattatcgacatgaag aacgacactgcaagcatggcataaagtcctgtcataccaccgaacatgataattactccacacagtatgatatttacta tagcgtatgtaagcagcatatcctgaggtctgaatttcgacattagcatagtacctatccatctgccgccaaggaaagc cagcatatacagtccgaagaatgtggtcgcctcatcctccgacagacctgcatacatgcagcagtaaactaggaaca ggctgttgatggctgtctgccctccgttatagaagaactgtgcgataactccccatctcaggtgtttgcgtttcaacactgc aaaattgataagcttgcccttctcgccgtgcgattcctccttgtcaatatcaggcaacttatacagtgcaaacaccacag caagaataatcagcaggactgcaagaaccagataaggcatcttcatggagtctgtctccatctgaataaatccgtccc aacctccgggaaagtcggcaggcagagtctcgcgagtatagttctgtccggtaagtataagcttactcagaaacattg cggatatgaaagcaccaagaccgttgaacgactgtgcaagattcagtcttcttgaagccgtatcgtgtgtacccagag ctgtcacatacggattggcagcagtttcgaggaagcacattcccgttgccatgatgaagaagattacaagatatgccc agtattcctttatctcggctgcagggaagaaaagcagaccaccgatggctgcaagaatgagaccgacaattataccc gacttatagctgaaacgtttcatgaacattgctatcggtatgggaaacaggaagtaggccagccaataggcagcttca gtgaacgaggcctcaaaagcattcagttcacaggttttcatcaactgcctgatcattgtaggcaatagattactgctgata gcccacatgaagaacaagctgaatatcagtaaaagcggtataaaatatttgtttttcattctgacatgtttttaatataaggt aactcaggcagattcttgaaaccgtaaaaggctttcgcgttctcgcccaagaaaagttttttgcttctctcttccaattcttttg atttaatcacaaagtcgtacgacatcttgtaggtaatggctgtgattgtgcgtggatagtcggaaccccacatcagtttctc gaagccaacaaggtcggcagcttcgttgatggctctgacagcgctgcggaacggatagaactcgtcattgaacagc caagtgataccgcccgactcaatcatcacattcttatgacgggcaagcattatctgcttcttccaatccggtttagtcacc ataccgaaatgcccgatggcaatcttcaagtacggacattctgaaatgatttcttccatctcgcccacctggaggtctcc ctctgccatatctatggaaagaatcacccccttgtcttccattagatgaaacatcctcatcatctcgtccgagttgagcatc accctaccgtccttcagttgcaggcggtgtcccggaatctttatggccttgaaccctttgtctataagttcaaccgcctggtt atagaaacccggttttctgaattcacacataccacacacgaagaacctgtccggatatttcgtcatcacctccatcagat agtcattctgaatgccgtcgatatactcctgtgtgacaacagccgcgccaatcagggcataattcatattagccaggaa aacctcagccgtgtttcttccgtcaatcataaagggggggggagcatttgtctcacctcccccataaacaatgattgacc gttctctgtagtcttgattttcaggccatctacttcagtgtcctgataaagccacagatgcgaatgggcgtcaattattgtata atccatagaaacagtatttatgaatttgcccaacttactctttgctgatcgcctattatctccttaaccttttccacaaggctcc agtctatcggttcctcaatgtattttatgttctgaagcacagactctgttcttgccgagctgaacaatgttgtaggtattctcgg attgcttacagagaactgcaccgcaagtttctcgatagggtatccctgttcagcacaatacttggcagcctttgcacaca cctcaatcaatggttttggagccggatgccattcaggaacacctctatgtgtgagaagtcccataccgaacggcgaag cgtttatcactcccacaccattttcgtcaaaatagtcgaggaagtccaccagcttgtcgtcgttcaatgaatagtgacaga agttaagcaccgcctctactgtacccggagcggcatggtcgataatccatttcaggttttcgagctgcaggtcggtgata cccacgtggcccaccacgcctttcttcttcagttccaccagagcaggcaatgtctcgttcaccacctggttcatatccgag aactcaacgtcgtgaacgttgataaggtcgatatagtcgatgttcagacgttccatactttcgtaaacactctcctgagcg cgtttgtccgagtagtcccacgtattcacaccgtccttgccatagcgtcccacctttgtagaaaggatgaacgattctcttg gcaattccttcagagccttacccaatacggtttcggctttataatgtccgtaatatggagaaacatcaataaagttcagtc cgcgttccactgctgtaaaaacagactgtatagcgtcactttctttgatagaatgaaaaactccgcccaatgaagatgc gccataactcaatacaggaaccttaagtcctgtctttcccaattcacgatattccatttttgataaataatttaaaggttaata ttttttactctgtttattcttattcatacagatagaacatacgttccatcatcttccatttctcgtccgatgtggccccctcggcac actgctggaatttggctacgtattcttcccattcggcctgacgcggcagagtggcaagctttgccatagctgtatcccagt caaaatccagaggtgtttccactatcataaagagttttgaccccaatatgtatatttccatttccaggattcccacctcgcgt attccggcgcgtatctcaggccatgcctcttccttactgtgagcctttctgtaggcttcaatcaattccggattctcacgcag actcaatgtctgacagtatctcttcacaggcagggaataacttttcactttatatccttctgtcttcatgatattattgatattaat atgttagtattacatgtcactgtctttatcttttcgacgatgctaaagtatgaagtatccatcaaaacaatagaggagattttc aaaaaagaaagaggggatattataccccctctttttcgacatttttacccctcataaaggagataaaaagtcaccccaa actctataaaaaatcaaaacagattgaactgcattcctgtgtagaaaaatccctggttggatttcggattccaatacgtca tcaccgtcaacgggatttcatattccataatccgaagtttataaatcacattcagggacacctgagtaattcctgccgattc ggcatacatggttctgttcaccatttccccgctttcatttcttgaatttctcaatgcgaaagctgttccaataccaggaccga cccttagcttttcgttctgatagatggtatagcccacatatacgaaactggagtagatgttcttgctgttgtccagatccctgt cgcgaccgtaaacaagtgtagagaagctcaactccagcggaaatttcctgtcgcccgtataattgaccatgagatca acgaaacgtccagtttcatcaggcttatagttgaagaactccttattattatatgtagccccgggcgagaaattatatgtat ctatagcctttatctgaaacctgccatgagtatatgctatatactggctcagctccttataactccccctggtgttcgatccg ccaaggaaaccggcggtaaacctccccgatgggtcggaaaccgacaaatcggacgagagaatcagtccgtcggc cacttcaatgccacgccatagaatcatgttctgtagagtagtactgaaatgaagctgagcctgaacatttgctgacaaa aatataaatacaggaattaacagtcgctttttatacttacaggtatccaatgataatatatgtatcatactcagagcagtag aaaatcggttttaaattattattatggatttatttgtcgaaatactctataagattataaacattccagttaatatccgacatgt atttggtcaatgatgtataaggtttatagttataatcgagcatacctttattgcaatcctcatcatccagatacttgaagaaa acccatcctacacaattcttggcttcgagcagtcccaaggtaaaatgctggtaagcgaatccacggttttgctggtcgcg taccacgaaaccagctccacttgaattgtcaagcttagtatcctcacccttggtatagaattccgttaccatgaaaggagt accgcccgcctggttcttccagccatccatgtagcctttttcaggcgaccatttactataataatttatggaaatgacatca caatattttcccgctgccttaattatataactgttgtatttaggaaggctgtgcaggcgtgaacccagataaagcaattcag gatccttcgatgccttaaccgcattctttatggcagaataatatttttccgcacaaataccggcaaactcattgttcagttcat ccgttacatcagaaacatttgcactcttgtccttatccgtcataaacttggcggctgcaatataagcaggatcctgcttgttt gaaattttcaggaatctgtcgagcagcctgtttccccatgtagagaagtctatctcattatccgagaagaatcccaacac atccgggttgtttctgaacatgccgaaagcatccgaattgagatactccttgcaccattcatcccatccatcataaaaca caagacctatcttaagattcacgttctgccccggatagctaattcccttgctattcttgaactctgcaaggaatgaaaagg aaggagcctgtgtcagaggacttgaagccgatttattataatcatttacagccttgtcgccttcttccttaccgaaagcgc agacactatgaaatcctatttcagagaattgtttctgcgactttgccacccagtcatctactgaactgtaaagcttgccga aagctgagctgttgccatccattctgaatgaggcgataccccttacataatatggataaccttcggggtcgactatccaa cttcttccatttgagtttttctcaaccctgaaccgtccagtagccttggatttttgcccttttgcgtatgagccatatttattcacg ctttgcaaatactcatcctgtgtttttgtctgctgttcataaccaaccaggtatggcaatatccttgtctttgcctctataaaagc cttgtcaggtttttccgcatactcgacaattatcggttgatactgcttggtgctattaggataggtttcagcaggaccgggaa caggcagttgcagttctacatcatcatcgtcattatcgcctgcattgtcgccgggagtattatagtcctccacattccccgg ttgtgagtaaataacctcaggcggaatatatgagaactcctcctgagggtcttcacatgacaaagcgaagaacggaa cactcaagcaaatggttttagtaataatagtagaatatttcattgttgcaaatatttagtaaattaatataaatcccatgtcct gattgtatccccccatcggtggtctatcgggaactccatttctccccatgccttaacagaagtccaaggttggtcggcatc agtccagaatgggtcagaggcaggcaatcccaacggaaggaatgcaagtgtagtcatatacaggctgccattgtttg tataatgattcgaaatgccagtctgatgtccgcagaatcctatggtgaggaatccgccctcattgaagttattgcccgact tgaacatacgtttcatacacgctgtcagcgcacatctcacctgtgctttcgatactcccgccggcaactcattataccatg ctataagagccagtggctgcattgttgccatacggtaaggtatagagcgtccgaaaacagggaatgttccttcaggag atatgaaacgctccagaatcatggcgaacctctgtgccctcatcaatgccctgtcatagtacttgcgatagtcgaaacgt gtcctcacgcccgattccattattgcatgtatagattcgagatacataggatggaacacataactgctataataatcgaat gcaaagtgctgtccgtctgcgtaccatccgtcgcctacataccattcctccaccttgcggaaagtagaatttatacgatat gtatcctgtccggcatcaattttggcaaggaagctttcaatggtggccgagaacagcagccagttagtgtaaggaggg tcaatgcgtcggagacctttgaactcttttatgtagcgttcctttgttgtctggtccagcggtttccacagctggtcgaacgcg cgcaggaaactttccgcaatataggcagcatcaaccagtgcctgaccatgaccgttccacaacagataatccggact attagggtccaccgcatttgcataactcttcaatgcccattctttcagttgcttgcgctgctgtccttctgctgtatcatcgtca ggcaggctcaaccatggagctataccggccatgagacgtccgaaagtttccatatatgcaaccttcttgttacggttatc ccagtttggacttacctcaagaatcatatttttctgcagttcccctttcgccatattgctcaacacaggagcagccatcctgt aagccatatccgtccagtattttcttgtctcgttgttgtttgcctcgagataacgcacatactcgcaagcggcaagaagga atgcgcctaccccaaagttggcagtcgacttggcgtcaaccacctgtcccggaatagccttttcaccgattggctggac ataacccaccgaccagtctttctgcagtgcagtcttggtaagatatttccatgctttccccactacaggcataaattcatcc ttgtcaagataaccgttgtttatcccccaaagcataccgtaagtgaagaaagcggtaccgcttgtttccggtcccggagc atgttccggatccatcatacttcttgtccagtagccctccggctgctgcagacatgcaaccgcctttgccatacgcacaa acttatcctcgaaaaaagacagatgctcataaccctccggcaggtccttcagcacctttgccagagcggcaagcacc catccgtcgcctcttgcccagaaatccttctttccgttcagactcttatgcttgggataaacatattttgcgtcgcgataatag agtccttcctcctcatcatacattattgagtccgacgtacaaagatattcatacagtttcttaagataccggtgattatgcgt aatcttatacatcttcgtcattaccggcatcaccatataaagtccgtcgctccaccaccagtaatccttacgcggtgtgctc atctggtactccatgacttcgcgtgcacgcttgattttataattctccggcatgacgttatacaagtccgcataagtctgga agcacacctgataatcgccgaacagcacataatcatcctttaccccgtatttatacttccattcagatttgttgttgcttttcg cacccatccactggttatactcagcccatgcctccgaatactttctgtattcttctttcccagtaaggaaataggcttccatat taccggtgtgatatgccgcataatcccagaaagaccttgcttcgggggcatgatttttctgccaggcatcgttcactttttca atcatctccctaacttgctgagcctcagtttttttttgcgaaggaaaatgaaggtaaaacagctataaggatgtataacat ccagtagtatctataacagttcatctttgtgatattgtttacattttctaaaacgaaatggggaagaatatatattcctccctc atttcacgaataattgtattattatatttatttgttaggagtccattctgctccgttgttgaaaccttctgttgtagagtcaaaactt gcatctgctcctgtacttggtctttctgtaatttcttcaatcttaaaagaagtgattttagcggttccagtagcatcagtaccac cagggacattagtctgtacagttaaaataacgttctcaagaaccggccacacaagtgaaccatctgctcttgaagctg gagtttcagcagaagtagaactactgattgtgaatgtatttgtataggttccacttccggtatttcttccaatccagaatttat atttatcagatgctcccaatctgaatgttgttgcacagtcgttagatgcgtatgtataagtaaatttgtaagtacaaccatca cggaatgacattgatttagtaactgggaattgattatctgctggaacaatttccaattctccacttgcattaattttttcggca actccttctgcaagatattccttaactgcatctatgttagcgaagttaaaatcaaaagcatctgcatgagtcaaagcaac attggcagattcaatcttgatattagcatcttcgttgttttcgtttttagcagtcaaagcactaacagcataatcagtgttataa cttacgctaatatttgcgtcattactataaatcttatcaccaagaataagagtcatagtagttccattcacagaaccggaa gcaacaggaattgtttttcctgctactgttatggtaaatgctttgttaacagcatcagtgaatgttccagaaacttccttatcg agtgtaagttcaattcggtcattacctgttgtctgatcaggaacaatttctttagctgaagaaacggcaacagtagtttgtttt tccaaatccacaggaggttcaccgcctccttgatcatcattcaatactatcgttacaatctgtcctttagtaactataaggttt tcaccactgaagttataagttttagtaccagaatttcttgtaagttctaaagtaaatccatcggtaaatgtcaccggagcta caaccattgagtattccttggcatttttattttgttcattaggaccaacaaatgttccctctttagcggttagagttataacatta gaaccggattccactgtcaggtttgctgaagcatcaatttttacgttccctgcaatctttacatcaccaccagcagtaagttt aatacctgtaaggtcagtaagattatttttaaacttaaccaatccacaagtattctggaaagttaaagatttgttattatctgtt gcagtagcataagatatatttgcatttgcatcgaatccccaagccggagctgtctgttcagatggcagtgtagtagttacg acaccttcaagacacacagcttcggcattataaggataaagagctgtatatgaattgttaggtgtagccttacctgtaaa cgttgtaactgtgctaccacctgtagcggtagtaaacttgttattttcttggcctgaaaagatattgattgcatctcctgttgtc caccacaccgttgttccattctgcaacgaactacggcttgaaggcgtaccggcaacaaaagtcatatcctgaggacc actgactgcatttacattcgacagttcgtcttttgtacaagactggagcattgcaatactcatcaaagccgctccacaaaa tagcatcgtatttttcatgacataaattatttgttaaacagtttcaataataaaaaatcacatcacttgttattcatattcttattct ttaggatcaggtttccattcagtaccgtcatcttcaaaatcatcatgaccgccatctacaattccgggaggtattgatattc ggcataccgcactttttattccattacccgtatctacagaagcaccgatattagaatctctgcccccgtcgattgccacga ccgtacatctcattttatcgtccgatggtgtaatcatcaacacatcagggaaagaagttccccaaactattgacttgtaac cggtataagggagattatccttggttatattaatacccaactccacagtgccactatatggtaattctatataactgacagg tttgttgtcagtctgcccatccttgaacactacatattcaatttttatctcctcagctggtgttccatcacctccacctacgccat catcatccttatcacacgagattgccgtaaactgtataaaaagaagtatgaaaaggttgtatactgacagaatccgtgg ttttatatcaaccataataaaatgttatttaagcgccaaacaaaattttcaatattcaaaaggcataagaggaaaccctg aatatgccttattaccatgaaaacaaatcaatctacctttttcaatccggaatcagaaaaatatgttatttatttagaacata tttttccgatttgccagattacaatcacaataaataaatcaacaactaaatctaattacctaatcttataactaaaccctca aacaatgttatttaaccttttctatcttgacatcatcaagcaggaagcatccaccattacctgaacccggaacagctgtga aacgatatacaaaaccattttcctgcaatttgaatttaactgttgtaagattgtaattcttacggtctttcttgacctcagcagt ggcaatttcttccagtttctttgaatccggattatagtactcaatcctgaagttaggtttgtcaccccaactgtatttggtataa gctgaaatctgatattctgctccagtttcatagctgatgtttacagcctgccacataccaaccttcacctcaacagcatagt tgcctgaatgtgcctttttcgcatcaactattttgttatctttcttttcccagacattccatgatgtcaagtcacctgactcaaaat caccgttcttaatttcctgagcgtatgcagaagtcatcatcattccgcaagccatcattgctaaaatttcttttttcattttttcta aggtttttaatttaagtattatgttgtatctattaaaatcactcttctattggaaccaacttataagccctgacccagtcataat aagtagtacttttgtccttatccttcaagtcctcagctgtaggtacttgtttttcccaatcgtatgtttcagtaactatatgtatga acataggtcggtcaaacggagtatctgtatattttgttgtaggcttgatagtgtacatatactttccgtcataatagaatttca cggtatttgcatccacccaccaacaaccgtaagtatggaaatcttctgccgatgggtccgtcatatacgaaaccacatc cgaacgtttcgccgtattgtcagtacgtttgcctccttgttcctgataccaatagtgagtattactgttcatctgcatattccatg tcttgttccacggattatcagggttgacacttcttattatacccattgtttctataatatcaagttcctgactgctccatgtctttat cttcttgccgcctttcattatttccttcattaccgggcggttggaaagccaaaaagtagacgacatggtagtgagcgaag ccttcatccttgtttcataatacccataatgtgcctggttctttgcagaagcaaccgctccaccggcaagacgatatttatc gcccggctttccatcaagtccttctgttggcgacaaaacggtattgattatacgaagacaacctttcttgacactaacattc tctgccttgaaagttgcaggcggccgaccgttagtccaataaggacttttagcatgccatttagcggcattaagacgttta ccattgaattcatcagtataatcttcgttaactacccatttataaccctcaggagcctcaggcaaattttttatatgctcttcag ccaaagaatattccttatcattttttaatgtataagatgacaggaataaagatgcagcagataaatacaatactgtttttctc ataaactttgtcgttttagattttttgttacacgacaaaagtatataagtttcatgaaagcattaagggggatttacatcgtaa aaggtggggtaaaattctaccactccctgaaacacaattatttcactcatgaaaccatgtgtttttacgatatataaaacc cgacagaagaataataccgtattaccggctaatttacataagaataacttttcaaaccgccatataccccactttacgtc cgtaccctcagtcctcgactccggcaatatgttttccatatcgagatctatggttttctgcctcggattcaaccactaactgtc gagcatgtggattgcgtatctgtcatagaatctctttccgaaccatattatctcgtctgtgctaagtatgttgttcagacggat aatctttccggtattttaccacctacttctcttgcaaatcctgatctgatataaccggatactctcaattcattgatttccgactt gtatacagtctgcgaagaggcattgaaactactgcacagactgaacagcagcaggggaataatttaactgattttaat agtagacattctgtgttcataatatttcattttaatgattacgtttctgactttcgtctgatgcaaaattatgaggtatcggacgg ggttgtatctttcagtaaaaatcagtaaagtcttggcaaggggtaaaaaacttaacatcttgtatataaatatattacaaa caaggtgcaaagattttcagtaaacgatggcgaatacagaacctatatatttacacgccataaaatgaagaaaaagc agtaggaaaaaaatgcgggcaagttccggataaaatgtgggcaagtttaaggtaaaacttgcccgcattttagatag aatgcgatcgcatttaaaacaagtaaaaaacgaagaaaaaaaatatgtgttcttcacagaacacatatttcaaaaata ggtataaacacgctaaacaatgttaacaaaatctatttataaaaaaagctcacatcaataatatctgcaacatttttaca atactccataaatgaagagaccttgggatgatttatacacagagctatctgtgatgtaggcgaaaaacgtcctgtcccgt caagaaacgctgtaagctcagatgggaggagtatactgccaatacctggatttacgtcagtcagaacgactgtatttac agcttccaccgctgacacatcaagataatcgagtgccggaagatctgcgaagtgcaattttcctatcatattgccgccttt 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cacgagattattgtgaaacagagaactatcagtacataaaagctaataaaattcctcataacaatcagttttgtggtaat aagactatattatcaatccaagccgcgtcgttctgtctttcgcacacaatggcacacactacttttttcactgtagaattaaa atcgaaagatacggctttataattgccgggagaagaaaattcctctgtatataccgttcctgtagacatatcctgtagcat gactttcaacttacatgctccttcggtctttacatcagcagagaagcgataagtcctgccactctccatgtcaaccctctgc atgagtcctgcatgaccagatatacaggctacattattgcctgcattgtcagtctgtacgcaaaccgtaccatagttaccc aatggctgccatgctgaaagtccttcgctgaaggttccattctgcaaggtagagacagtatatttctcaacctgcagtatc atggacgatacgtgacctcctccgtcggaaaaggtgatgtcgacattattatcgccattcttcagcagctgtatgtcgaa cggtacttctatcataccgaaaaatatattgcggttgctctggccgtagcctttccagttgtcgggaacactcacagcggt accattaatctttaccaccggtttcttggaagcagagacaggacggcctatcgacatacgcaagcttgctctgcccgaa ccggactcgattcctgtgaaggggaacgaaagggatgatccggcggaaatcggtttcagatactcactgctgtaatatt tattgcggattatggagttcgtgaatgctgacgaagacacatctgctacaaggactatggtctgatttgggacaattgag atgctttcaggcatggacgggacattctgttccgtatattctatacctgcgttataattgacatatagagaacgctttgtgac 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gctgtatggtttcatctgaggatatttgcctacctcatgcgtcttgttatacgcatacgagtatggtccccagaactttcttcca agaccgacctgatagtcggcaagaaacttgcctacatccttatcatcatcggaggtggaatgaatattgaaatatttag aacggtcgagttctgaaacaccgctcaaaaagcgacgggtattatagtcgacaaccacctcgttcctttcctgacaata aataccgggaggaacacctagggtaaatgccgataacagaaaaatatatttatagctcataatttctttccttttagaca cagaaacttgtcagtcctgatgtggatacattattttctcactttcttatcgtagcgttcagtctgaagaatcatagtagccac acggcctccattatccgggaatgttactgacaccgaattttttccttttctgattaaccggtagtcgaaaggtatttctatcat accgaagaaatcgtctctgccggtctggtcatatcctctccaattgtcgggcatgtcgactttcttgccattaaccattatttc aggtttcttcgacatctcgtgcttcctgcctattgacatacgcagaacagctcttcctgtacccggtttcagaccatcgaaa tcaaacacaattggttttccggcttccaccggctgaagataagtgttgctataatatttagtacgaactattctgtttgaatac tttttacggatgatgtcggcacacaatattattgtctcatcttttataatgtcaatactttgaggcatcgagttcagcgtcttttca tcataaactatacctttatcgaaaatcatcttcaaagagcgcacagaaacattatctacacccttccaattcagtacgttttt caagtttaccttatgtgtatagtcatcaagattgtcgacagctatgtaaagcctgtcatcgtccttaaaagctgccacctgt atgtccggattgtcggaaacaatatctacacgttcgcctttcacatccttccataacttgaagaaatatttcttgtcgttcagt ttccatgcggtattcttcaagtcgtgaggattgttggcaacaaataaagcagctccgtatggttcgaaattatattgtttcgtt atatgccattcggccttgtcagaaacaaagggtattgagatgagcatcttgtcttcgcgttcaagaagattgaacagtat atgattgaacgaagcgacagttcgtacagaggctatcggattatatcctttggaagtgttgtctattcctccatattcggtta cggcaagaggaagaactttccccaagcggatgaacgagtagttttccataaggtcgagaatagcttcggaattacttc ccgaacggcgggaactcttgcctactatgtttattccatcgtaaagatgtaccgacaagccatccatgtactccccggc acggtcaatgaacatcttcatagtattattccaatggtcgaaatcgcgcaactccatagccggatatgccgcggcatatc caatgattttcatttttttcagacttggctcagcgtgaatatgctttcctgtctgtgcataaaaatctgccatgagcatcctcatt tcctgaccatgcatattgaaacatttgtcgcgtgcatggacaaagggttcgttaatgggttcgaaaaattcaggaactgc ccctttcacatgcttggaatagtattcggcagcccatgcacccgccttcactgggtctatgccccattgtatggtacgcgc gttggcatgttccgtagcgacatatcgttttgtttccttcaaatcagtgtagttcaaaggcttttctgaaaaaggatattcgcc aaccttttttgtcttgccatatgaataagagaacggtccccagaaagagcggccgattcctacaccgtaatctgcaaga aatttcctgacatctggatcagaatctttagatgtgtgtatattgaaatatttacctctgtcaagtgccgatacatcattcagg tatctctgagtggcataatccactgtgacagtagtgttataagtcttattctcggaagatgataaaggaaaaaccgagaa agacaaacacacagacaaagctgtaagaattatgttattcattgtattatcaaaatttaaaaggcagagaacactccg atagttcaattaaagtattccctgccattaagattatcacttctgtttaaacactaatatcagaaatcggccggtttgagtac atcgttcagcaccacttcatattcaacttctgttccgtcgttttcagtaacagtaagatggccgtaaccgccacttgagttatt ttctttcttaccttcaaacatgaacattctcttcttcgtcacttcctgttcttctttatcgcctgtttcaggattgataacttcttcctttt cagtatagacttcattgaaagagaatgagagatgtttttctgtatccgaattgattttcagccactcgggcaattccgaag gagcctcagacgactcggcaaagaactcgatcttattcattctcaaagtctgatagtcattcttccaggcaatgaggtcg aacaattcgcgataaacagaaaacttggaaacctcgcctgtctttcctgtttccacattcttgagataggtaagttcatac acgggagtagaatcaagttcgacctcagcccatttgtcattatcacatgctccgaacaaaaccaaagcacataagaa tgtaattgtcttataaattttatctattagcttcattgttactataatttattatggtcttacttcaatatatccgaaaaatatatcgtc aaaataaatattatccttaaaggcattaaagcgcatactgagcaatatattgtccatttcagcctttgaagtcacagtggtt gtggccgacatccatttgctgtcggagccattcacaatgccgcaccatggtctatcgctctgccatgtcatatcttcagctc cttctttacctgccggaacgaaatacggactcatacccttaccctgtttataccccggtgtataatatttgtagctgaaagta tatgtacctttaccaccagtaaatgtcttggagagtaatgccctgcatcggtcaaatgcttcgacaaacatacattttgca ctgttgtttattccatccttcagaggattgtccacaacctgtgaaggaactacaggatgtgttttggtatcggcatcaataac tttccagtcggcatatgtgtcagaattttcaaaatcttcatccaggaacgcaccaaaagtagtcgctacgtttggagctgt agcctttatctcaaggttctgatatccaaccaacgcttcagttaaagttcctgtaagggtcagttcatctgtgttatagattttc tcaaccaaagtaagaatcagttcatatctgctttgcttgtttacttctgctgctgtgatgtttacgctacccctgacagctgac ggtctgttatacgagttggagtaagtaagctttagagatgatggatttatctctttatatccaaactcagaattatccaaatct atagcaatgtgtgtttggtcaatctgacggatgttataagtaataggatcatcactaggtactactgtaatagccaaaggc acaacaagagtttttggcgaagcttttggagtgtacttacctttaccctcactggcagaagttctttctattgtcatggaaag aagcaatggcttatcgctgaatttctttgcagtgaactggtatggagtgtcaaaactggttaattcgtcatttacgccagtat ccgcacatttgaaagtccatttgttaggcaatccgtatgaatcgtccttaatatagacagacttaccatattcaagttcgtat ttttcgtattcgggagcttcctcagttccaccgactattccggtctttatttcctgtgtacactccggatcactgtatacctttac ggccggtacgaggttaggatcatacacgcggatatggaaagttgtatccatcacatatacatcaccctcctgcttagca taacaatattttttgatatatcctccggtattgtcgtcatacaccgaatatggatatacaacctgtctgcggaaagtattgca caaacgtaccgtatggtcaccgggtttagtgaaatacacatgtatggttttcaaatcgttggtatgagggatggattcatc aatcaggtttgtatagtctgtctgtccccactccatcttaccattaaggaactttgtaccatcatccgacacaacccactga tgcgacaacatgccttgggataagtccattatacttatatagttattaagattcagctgaataggtgaaacgttttcctgatc tgtactcacatgccaggtacattcagccacgttattcaacggttcaaactcatcatccttacaagatgtcagaaccgaga ttaatgaaagagcaatatataaaaatctatttttcatcgtatttatttattaatatcaggatttgatgtaatttctatatttggaata ggccagtatgccacttgcggaccgtagttcaatgatgcttggaaataatccacaaaagcgtttcctctcttttctggcggc agctcataaaatctgtactgctttccaaagttgaatgctgataccaaagcattagggtcatcaggattaggcttaagatat ttggtctgaatcatacagtacttatattcgtcggatgccaactgatcaaacctttccttagttatattccagcgtctcaaatca atgacacgtatggcatgtccttccatacacagttcaagaggacgttccacatacatcagatgattcattacatcacttgca gcatattccttctcatcgtatgtatatctcttgaattctccctgttccgattttccgataagcacaactccagcacggtgacgt accttgttgatggcattgatagctgactgaacatttccatcgcttgcaccgcctttaatcagacattctgcatacatcagata tatatctgccaaacggataagacgatagtttattcctgaggccatagcaggcttaaattcagtttcactcttacgtgtatcc caatttgataattttctgaaatacgctgaagagccacggttgaattttgatacctgttgtgggagagactgataatatatca gactttcatcgccgtttattgcaagagaggcagatgcacgcatggaatagcttctgaggcgatatgcctgaccgtcttcc catttaaattccggaactatgtcatcgtagccggtaatcttattgtataaaactttattatctccgacagttgagacgagtcgt tcgcgtactccaacatattttcctgctgttgcatcccacgtataaacgtacgttctgttatatacgacaccctgacggtccac ctgcgagctgaaagttgttcccaactggtcgtatataatatccctatgttcaggatcaccataattgtcggactgcatttttat ccagttacgttcatcaagtctgtccaccggctctgtttcgaatgcttcaacaagccaaaaagcaggaacagtgttaagc caggcatcgcccaagccatttacattcattccccatatattatataaggtagactccgaccatgtaccgaattctgtattat actgtgtagaataggaaacctcgagaatagattccgaattgaattcattggcagcagtaaaattatcgactatgtcatca accaaagcaaaacctccattatcaataatatccttaaaatattcggcagctttattatactctttatcataaaggtagcttttg cctaatattgcctttacagcccaagaggtgatacgtcccaaatcggttttctcccatttgtcattcaagccaaggtcaaga gctttctgtaaatcttctctgtaatatttcttgatttcatcacttggtgtaacctttttatagtaatcttcttctacctctgcaatttcatt aatataaggaacattaccattattgaatgaattattgagataaaaataaaacaagccacgcaaagaatatgcctgtgc ctcaatctgagcaagcttggttatttgaggttcatctgtaacatttggacggattttctctatactggccagaacctgattcgc acggaacacaccagtatacagtgcagaccatttaccacggactgttccgtatgaatcattaaaggtttgcttataggctt cgttatcaaactgctttctgtccttattaccttcaactgctatatcacttctacggttctcatcgagcggatgataaatattggt atttttcaaagcattatatacagcagccagtcctttctcgcagtcgcctattgttttataaaaattctgtgttgtcagctgatgta tgttttcctgcgtaaggaaatcgtcgcatgaaaccaatgtcatgcccgacatcaacagactgaatactattgttttatatct gaagttcatatatttatattattaaaagttagaaattaatctggaatccgccacgcatctggatacttataggatatgttccat agtccaaaccacgacgtgacaatccattactaccgacctcagggtcgtatccgtcgtattttgtcagtgtaagaagatta tcggctgcaacgtataaacggaacttgcccaatccaagctttgatacccaactcttggggaatgaatatcctaacataa tatttttaagtctgacaaatgaaccgtcctcaatccacatatcagtatgagcacgatagttgttatgcccctctgtacgata agaaggaatggtagaggtatagttggtaggggtccacatgtatatcagttccttattggttcttctttgatatgtatatatcttc gtaccgtttattatttcatttccaactgaagcataccagttcatagagaaatcgaagcctctatagtcggccgagaagttc aaaccaagttcataatccggcataccactaccggcataaacacggtcgtcatcattaagaacaccatcattattggtat cgatatacataaggtcacccatacgggcacttgactgtaatttctgatattctgcaagcttctgttcagtattgattacccct gcggttggcataacaaagaaagcaccggcttcatatcctttcttgattgcagttacataatcacttcctgatgaaacaggt ttaccgtcggggaagaaatataactcattttttcctgccatagacacaatctcattcacgtttttggtaaatgtaccagtcaa gctgtaattaacaccacgtattttgttgcggtgagtaagtgaaaactcaacaccacggttttccatatctccggcattcaat gtaacagttgaactctggccccctccatttgacggtggcacgaccatcgggaaaagcatattcttcttgttactcttgtaca aatcaagacctaagataagcttgttattatataaagccatgtcgataccggcattaagctgctgggttgtttcccatttcac attcggattggcaaatcccaattgggtaaaaccatttgcaagaatttcggaagttccggtaccaaaagtatagtcgtagt ttttgtatatagctggtgcgtatgaataatcagggaagttctgattaccggtagtaccatagctgaatcttaattttaacgaat ttactagccacctgaatctgtcgaagaatgattcctcagaaatattccatcctacagacaatgacgggaacaatcccca acgattttcttcggagaacttagatgaaccgtcgcgcctgatactggcacttgccatgtatttgtctgcatagctatattgta gacgacccaacataccaaccattgtactgatacggtcctgtccccactggccactgcctgtacccacagtcatatcgg atgttcccgcatttaggttcggaatctcgttagtaaccaaatccattatactggcatagaacatctcgtatgtatatttctcca tactgaaaactccggtaaatttaatatcatgcttttttatcttcttattataatttaccattgtttcccaagtgagactggtattcttt gaatgagtatcttttaattgcgaacggtaattagagctggttaccttttcgcctttctgattatatacctcaaactcaggtcga attgagacagctttctgattgttatatccaaagcccaaacgtgtggaaacattcagtccgggaattacattataagcaag ataaaaattaccgttaaatgattctgtgtccttatgattttcctctttcaatcttcccaatgtataacttacgccctgtaaatctg caggatcgccagctgcatttactatacttgcctgtggataaatctgagaacgagtaggcgagtagtcataacattcgttc aataacccccaagccggagataactggttttctatcttcatagcgatgttagtgttgatagtccattttccgcgctgaaaat gtgtattcgaacgaatattatatcttttgtaatcggaatttatcaacacacctttctggtcgaaatagttcgcggtaaggttat atgtcaaatctttcttgccgccattcgcagtaacagaataattctgtattggtgcgttattattgactacatattcatataaact agagttgttgaagaaattcacaggatatgttttcagattagaccaggccaggtcgtctgtattctggtttccttccatcattct gttagacatcacttttacaaatatactctcgttggcatcaagcaaatgaatattcgaagtaatgtgctgtacaccataatat ccgtcgacagctatcttcatttctccttccttacccttctttgtggtaataaggataacaccggaagcaccgcgagtaccat aaatggcagccgaagcagcatccttaagaatatctatacttgctatttcgctactactcaatcccgggtcgccctcgaac gggacaccatcgacaacatataaaggagaactgtcgcctgagatagaacttaaaccacgaatctggatgttggattt ggctccaggctcaccagaacttgcctgaacgttaactccggcaaccataccctgaagagctgtacccaagtcggaa gtactgatcttagtaatctcatctgagtttacacgtgccactgcacctgtcacctcttttttacgcattgagccataacctaca acaaccacttcatccaacacttttgtgtcttcctgaagcttgatattataaatctgaccattcttgattgcagcttttacagtttt atacccaacaaaactgaacactaagttacctttagtcggtaccccttgaagaacgaaattaccatccatatcagtaata gttccaagagaagtaccttcaacttgaacagctgcgcctataacttcaaggttattggcagcatcaatcacctttcctttaa ctgttatcttctgtgaatacatagacaatgtatagaagataagcatcacgaacaacatgtacctgccatggtaccatttttt ctgatttctcatttgtaaaaattttaatttagcaataggttatgaaattccttttataactgacgctaaattatttatttataatggt acaaaaggggagaattatatatttaaaaagggggtaaaattttacccccacttatattaagaatccaaatcggtctgtat actctgttctttgtactgttgcggcaatacaccgaattctttcttgaaacattctctgaaatacttcaaatcattgaaccctaca tcgtatgtcacctctgatacagaataccgtcctgtcttcaacagttctgccgctctcttcattcttattgaacgtacaaaagc attggctgttactcccataagtgctttcagcttcttgttcagaaccaaggccgtcacgccaagacctttacatatatcctcta tctggaacgaagagtctgtaatgttgtcctctattatctttacaagtttctcaaggaacttatcgtcggtagatgtagtgctta cctcggaaatctttattgccggaactttcttgtgttgaagaatccgcttcctgttggttataatggaattaagcagctctttcatt atcttgttgtcgaaaggtttagggcaataagcatctgcatggaatttatatccgatgaaataatcctgcaatgtagtcttgg ctgaaagcaatactacaggaatatgagatgtccttacatcctgcttgattctctcacacagttccagaccattcatgcccg gcatcattatatcggataaaacaagatccggttgcaaatctggaatcatgttccatgccatctccccatcatgggctatca ttatcttatacttatccgacaacagtaatgacaacatattacatatatccttattgtcatcaacaatcaatatagccggagat tctccgtccacttctatgtctatcatctcttcatgctcgcacgattcacttcttaacacatcagcaaacttttcatcctccccact gttggcagagatattctccgtaaccatgtccccctcagttatcataggaattacaacatggaaaacagtgcctttaccttc ctctgatacaaacgtaatatttccattatgtatctctacaagccgcttggtcagaaacagacctataccggtacctccttca gcagagtttttattctgactgtagaaacgctcgaagaggtgtgttttcaggttgtcggatattccgtttcccgagtctgccac agagatgtttattttgttatcctgttcattgacagtaaacgatacaaatcctccggcaggagtatgcttaatggcattcgata cgagattatagattatctgttccataagatgagggtcgaacagaaagcttatatcactgcgtgagacagaatattccag ccctacacctttctgttttgcccaatacgtgaactgctgaaatacttcttttgagaaagacgagaagttgccatatttgagat tcagactaagcattcctttctcgctctttgagaagttcatcagctggttgacaagacttaacaggaacttactgttatgctcc attgtctgcagcatgccggcaagatacttgtcggacgaatacttgcccgattcaataatcatactaagtggagaatgaat aagtgtgagtggtgtcctcaattcatgcgatatgttggtaaaaaatgtagtctccttttcaagaagttcttcagtcttgcgttttt ccatgtttgctatatatagagcatttctgcgctgcacccgtgaggtataatacaccttgaaccggtataaagacaagaca agcaatataaaatagagtgtataggcataccatgtacgccagaaaggagggttaataatgacaggtatggaaagttc attcaaactgtagactccatcgctattcctgaccctcagtctgaacatatattcgcctgaaggaagctttgtgtagaaagc ctcacgatgaaaagcggaggtggaaatccatgaatcatctacgccttcgagcatatattcgtaaccaaccttataagg acttctgtaatccagggagctgaactggaatgagaaagtgtttaaattataaggcaattcaatgtgctctgtaaaacttac acttttgtcgaaataagctgaatatgtggaatctgcctcaacgctgtgattgaagattttaaaatcaacgagtgtaggact accgttgaaatctatcacatcaaagtcattaggtctaaagacgttaattccgtttacgccaccgaatatcattgttccatcc gtcattactccagcagaaagttccataaattcataatcctgaagaccatcgaaaatatcataagatcttattctctgtgtgtt gatattcaacgaattaattcctttattggtagaaatccataatgttccatccgtgccattaacaattgattttattgtattgctgc tcaacccgtctgcagagctaaaattttcaacgcaggcattatggttttcatccaaatccacgattttccttaacccacgtcc aagtgttccataccagatattatgattcaagtcttcacatacaggcactatatagtcgagttcatcaagtcccttgactgag ttcaaaacaggattatctatatacaaatctgcagattccaatactttaagaccgaagctggaagctacccatatattacc cttatgatctttaatgatgtttcttactatcttaagttctttattgtcagatgttttgatttccttcatcacacctgtggacaaatcata tctgaaaagacctttattatatgtgccaatccacaaatattttccatcggcaagcattgcgcgcacatttctcaaacctgag atctttttataatcattatcagaagtgaaactgtaaataccatcgtacatcagagacacatacatgcagtcggtgtagtttg agtatgctgttgagtatactatcctgtttgccgtgaaaggaataagtctggcattaccggtaatggaattaaaatgatata gccctgagccttctgtgcctaaatatatatcagatttggcaaatgtataaacggacgatatatgatcatttcctattcctctg aataaatctataggtttattattttcgcgtatactcataaagccactcttgaaaaatcctatccaaagaatatcgtttttatca agaactacagtttgcggatagctgtaagaatatgtagcaataacctgtggttttgactcgatggcatgcaatacatcaaa agtcaacacattcacagtgcttgtagtggcataaaataatcttttgtttttatataccatttttcgtatatcacagttttccaaca gggtacttaccttgcaggtatgcttgtcgtataaacataattgatgattttccagatttgagtacaatatttgagaagatgag atgactatggctgaagctatagggcatcccaatagtttgttaagcagtaattcatctccatcgacgttacattcgtacagg ccgtcttcggaggagagcattatcgtattatctatttctatgatgtcggaaatgtatggtaattttaatgttgatcttaagacag tatttattttgccattttgaaaatcataatttacaaggtatatactttcatcagaggaatgaaaccagactctgtctttagagtc gacaagaatcttatcgcaagtgaaatttttatcaataccgctgtgaccaagatttaatgaaacgaattcgttctttacaga attgaacaggaacactcctctatcggctgtacctatccacagatttccatgtgaatcttcgtcaatacatactatcagatta ctgttaagaccgtttgactgatatccgtaaaccttaaattcatatccgtcaaacctgttcagtccgtcgttcgtggccaacc atataaagccttttgagtcttgataaatacattgcacatcattttgggaaagtccatcaagagtagtgtactttcttgtgaca aactcattggatgcaaaggatttgcaaactataatcagaactgatattaaacttaagattaatctaaacatataactattat tctttatatttcatcaagattacaaagttattgattttatctaaaacatcaagtatttacagtagttaatagataattatagatatt ttccactttagaatgcgtatcaaaatcaatcaagaaaaaaataaatctttaacttcatttcatagtataaaacaaaaaaa gcatcgtaccattacactcaataatagatacgatgcccgaaagaaattacagtaacagactgtattgggattgttcttaa aaagacttatctgtatgactttatatatatgtcgagtatttcggtatccgacagttcatgagggtccagactgaacaatgca cccatggcagttcgcgcattatcaatcatcttagggaaatcttcctttactattccccagtcgctaagcttcaaatcgcgga cattgcattccttctgcattctcaccaaagcatctataaaatgttcgggattaaggttcttgcatccggtcataacatctgcc atgcgcatatatctctttgtcctgtcataaataaaagtagagaaataggcctcgcttatagctatcaggccaacaccatg aggaagagcgggatagtatgcgctgagagcgtgctcgagagaatgttcggaagtacaactggatgtggattcaacc attcccgccagcgtacttgcccaagccacctttgccctcgctttcaggttatttccatccttcaccgcaacaggtaaatattt atacagcagtctgatggcctcaagagcgaaaatatcacttattggggttgcacaattggcaatatagccttcggctgcat gaaagaatgcgtcgaatccctgataggcagtcagatgtggcggaactgaaaccatcagttccgggtcgattatcgac agacatgggaaagttaaagtggagccgatacctatcttttcgtttgtttccagattggttatgacagtccatgggtcagcct cggttccggttccggctgttgtaggaatggctatgatgggcaatgctttgctgtaaggaagccccttgccggtacctccttc aacatattcccaataatcgccatcattacatgccatgattgcaatggatttggccgtatctatcgaacttccgcctcccaa acctataatcatatcgcaattttcctcacgacagattgccgtaccttccattacatggtcttttattgggttaggcaatatcttg tcgtacaccacggcatcaacattattttctttcagcagaccaatcaccttatccagataaccatatttacgcattgatgttcc ggatgaaatgactatcaaagcctttttgccgggcaatgtctctgttgaaagacgtttaagttcgccacatccgaagagaa tcttcgtcggaatattataaccaaaaacaaaattattgtccataaatattatcagtcagtcaacttactatcttaaagcctca tcaatcactttcttgagttcaggataagcctcatctgtatcgcccacctgttttctcaactcacgcagtttctttttcatgtcctta agaactttggcgtatttaggattatcagccaggtttaccatttcgtaagggtcgttcttcacatcgtagagttcgaaagaaa ccggagtaggaacaatcttgtggctgttcttcaaccatgacattgatttctgtccgtaacgtttgtcgtcgtaatgacggcc atagaaaagtatcagcttatagttttccgtgcggatacctatgtgtgccggaacgtcgtgatgaatcatgtgcatccagta tctgtagtaaacagcatccttccagttttctggctttttgccttcgaacacagaggcaaagctctttccatccatgtatgaag gttctttgccaccgaccatctctataagagttggagcaaaatcaatgttgttaatcatcaggtccgacttggctcccttgta aggacatctcgggtcgcggactatgaaaggcattctttgagattcttcatacatccatctcttatcctgcagatcgtgttcgc caagcatcataccctggtcgcctgtatatacgataatggtattttcccagagtccttccttcttgagatagtcgaaaagac gtttcaggttgtcatccacaccctttacgcaacgcagatacgatttcaggtaatgctggtaggcaaggtatgtattctccat ttcatcacctgtattgcacttatattccattacataattgcggatttcatgacggcttgagacagaagttccgatgaagtgac gaagtgaatcgttcttgcctcttgtgccttcggagccccatttgtctgtatcgaacaatgacaatggaacaggcacttcca catcgtcaagataatattcatagcgcggtgcgtactcgaacatatcgtgcggtgccttgtaatgatgcatcatgaagaaa ggtttggacttgtcgcgtctgttcttcaaccagtcaatagcaaggttggtcacgatatccgaggagtaacccattttctttat ctggttattaggccatttcttgtcagttacgtcacttgtaaggaaaatagggtcgaagtattcgccctgtccgccatgaccg ttgaatacagaataatagtcgaagtgcgacggttcgcatcccaaatgccatttaccgatcatggcagtctgatatcccat attatggaactcatcaaccagatattcctggtccggctgaagcacttcatccaaagtgagcaccttgttacgatgggaat actgtccggtcatgatacatgcacggcttggggtactgatggagtttgtacagaaacagttctcgaagagcataccgtc ccttgccagttcatcaattgtaggagtagggttcagtactgcaagacgacttccgtatgcgccgatagcctgcgaagtat ggtcgtccgacatgatgtagatgacattcatctgtttctgctgtgctgcgacaccaacacatacagacaggaatggcat aacagccattcccttcattatattattttttaaattcgttttcataagtcagattatcattgaaatagaacttgcaagacatatc atcgaatgattttacgtccttattctgcattttaacccattgttctgatttagccttgacagcgacctgagttgaaacctcatta ccgtcgactacacttttaagagtgacatttgcatcctctgcattatggtttgccacacgtacagtgataaggcatccgttatc aaccttatcgtatagcggtttggaaaccaccgcccctttaagcttaatcttgaacacatgtgcatattcagtaggtttgttctt agggaagtttactacaagaccctcgtcagtcatcttatagtcaatcttctctgagcttccaagcatttcaaccgactcaattt ccacgttctggcaatacttaggagcaaatgacttgatagtaacactaccatctgtccaagccagagacacggcatag aggttattgtcgcgtgtagtaaagcgaatgtcgtccgctgtatattcagtttttgtattgtctgtcatataacctgcggtgcctg cgttatgtccttcgaaagcaatcacccatggtcgtgagccataaatagcctcaccgttagtcttcaaccatttacctatctc ggcaagtacgttcttctgttcgtctgtaatagtaccgtcggccttaggacctatattcagcaataagttaccgttcttgctga caatatcaacaaagtcgtcgatgatatggtcaggactcttgttttcctcgcccacacaatagctccacgatttcttgcctac agaagtatcagtctgccatggatattcacggattctgtcgctcttacctctttctatatcgaacacctggatattgtcgccata tccgaatttagtgttaaccacaacttctttattccaatcaagagccgaattgtaataataagccatgaatttatagaaagta ggctggaacggatattttcccacagtccagtcgaaccatatcaattcaggctgatatttgtcgataagctcgtatgtatgc ataaggaactgacggcgtgaacgttcgttcgagccttcatacttaccacaataaggtgtcataccctgaccttcgggctc atgcagtctttcgccatacagagtgattgtagtgtcctgaacatcagaaggagtttccattccatattcatagaaccatgc attctcgcatctgtgagaagaaagtccgaaacgcagaccggctttcttggtagcttccttcaattcgccgattatatcccttt tcggtcccatatccacagcattccacttattgaaagtactgctgtacatggcaaatccgtcgtgatgctcggccaccgga acaatgtattgtgctccagatgattttaccactgccagccactcgtcggcattgaaattttcggctttgaacatagggatga aatccttatatccgaatttggtcaaaggaccgtaagtctgtacgtgatacttattaataggatgaccttccttgtacatccag cgggaataccattcactgccgtatgcaggaacggaataaactccccagtggataaagataccgaacttggcatcctt aaaccattcaggaatagtgtaattttgagcaatcgatgccgaatcggccttgaacacatcagtaccttttaaagatacag tagaatctacattaggagcgtatgtagaattgcacgacgccaacaggcttaatgccgcaactcctaaaaccgttttcat ggatttcttattcataataatcttattacattaaataatgacattaattttttctgtaagcaaagatacacttgagttccatttaca ataaataatttaattactatagtaaggggtaaaatatttaccacctattattgaacaaatttaccccctctcatatatgataat aaactgccaatatcgaattacaagtaaatatatatttcaacaaaaaaggtttagcctattattacacaacaatttcaccct aagaataaaatatatatagagtaaatttgccaatataacaaactgtaaaaacaaatttatgaaaaactatttgatttactt actcgcagcagtatcgtgtacaactgtagcagacctaaatgctcaagtcagtacaaaaacaggtaatgaaaccaca gaacttacaattccgaaaaagttctacaaggacagcattgatttcagcaatgctccgaaaagacttaacaacaagtac cctctttccgaccagaagaacgaaggcggatgggttctaaacaaaaaggcctctgacgagttcaaaggaaagaag ctgaatgaggaaagatggttcccgaacaaccctaaatggaaaggaagacaacctactttctttgcaaaggagaata ctacatttgaagacggctgttgcgtgatgagaacttacaagccagcaggatcactgcccgaaggatatactcacactg ccggtttcctggtaagcaaagaacttttcctttacggatatttcgaagcaagactgagaccaaacgactcgccatgggtt ttcggtttctggatgtcgaacaatgaaagaaactggtggactgaaatagacatttgcgagaactgccccggcaatcctg ccaacagacatgacctgaactcgaacgtgcatgtatttaaagctccagcagataagggtgatataaagaaacatatc aacttccctgccaaatactatataccattcgaattgcagaaagactttcacgtatggggacttgactggagcaaggaat atatccgactatatatagacggagtactgtacagagaaatagagaacaagtactggcaccagccattacgcatcaat cttaacaacgaatcgaacaaatggttcggagccttgccggacgacaacaatatggattctgaatatctgatagattatg taagggtgtggtacaagaaataagaaataacataatctgaaattataaaaggcagtcttcattatcagtatgctgatgat aaagtctgcctttttaacaagaagataaagattttaatctgccctatcactcatttacttcatccggatactctgtaagcgag tttcccgaattgcttatttcaatagagccgataggaagataattgaacttcttgctccatgcagagataccataatctcttct aagaataggcatcatgacctcctcggcacgtcctgagcggacgaggtcaaaccatctgtcaccctcgcatgccagttc acaacgacgctcataccatagaacatcaattacgcttttaaatctgtcaggatacatctgcattagcttgtcaacatcaat ataacttccgtcgtctgcatgaacatgcttctttctgagttcatttatgtaatacttcgcttttgcttcatcaggattagtacctct gagatatgcttcggcaagcatcagatacacttcaccatatctgatgacccttacgtttccaggcttgtttagattggggtttc ctatcatatcgtaatttttgaaaggaggatatttcttctgggcatatccctggaaatcaggcccgtaagagcctgtctccca aacaactttttttgattcatcctgaatattggcattaggtttggttacaagttcatcgtaagtaaatatcgccgcatcacgacg cacatggtcatccggaaggaaataatcatacaattccttagtaggcagacaaaagccatatccattatcataatcagg actatttttcaactgtctcggtccgcagaaagtcacccacatagcaccttcgcctgcatcaatattaccccagtttgtattac cagatttggtagaggtctgtatttcaaatatagattcctcgttattctcctgatgagccgcaaacaatttagaataatcatcc gtcagagtataattaccacttgaaattacatcctccaataaaggtttcgctttgtcaaaaatcttagcatcatcgttgctcca gtcagcccaataaagatagaccttggccaacagggcttgagccgcagtcttggtaatacgtcctttcattgtgtccggg aaattatcctttagagaagggatagcttcaagaagatctttctctattgctttatttacattttcgcgagtatctctcgtaaactt gaatccttcaggataaagagtctcaagactgataaagcatggaccataatatctcaacaattcaaaatgataccaagc acgtaagaacttagcttcagctttataaactttagcttccggactgtcatactctgaatttattacaagattacatctatatata ccacggtaacgagttttccacaaattatcggaaatagaattgacactcgtatttgaataatcctctatagcctgcatgtaa ggctgatcctgatcagagccaccaccagtacgagcattatccgaacggatttcacccataggtacaatggaagcaag tgcattacccgaagcaccacctatgtgagctaacggatcataacaagcagtaagcgctttgaacatctgttcatcggtc ctataaaaagaactttctgtttcggacattataggagctgtatccaggaaactgtcgctgcaagatgatgatgcaatagc agcaaacatgaggacaagaatattattatgtattttcgacttcataattttcaattttagaaattaagacttaaaccaaatct gaatgtacgggcctgagggtaagtaccatagtcaatacctgtgctaagaatattgccacctgccatatttcctacttcag gatccataaacggatagctggtgaaagtggcaagattatcaattgctgcataaattcttgctttattcagcatcaacttgttt attaatttagttgggaatgaatagcctacctcaagtgaagaaatctttaaatgcgaaccatcataaagataaaaatcgg atggtttgccaaagtttccattaggatctttggatgaaagacgaggcactccattatcatcaccttctttccgccatctgtca agatagaatgatggaaggttgctgcgtccgtatgcttcctgtcggtaaatatcagagaagactttatatccagcttttcctg ttaagaagattgtcatatcaatacctctccagtcggcacctaaattcaaaccgaatgtccattttggccaaggattgccac aatcggttctatcttcatctgtaatctgcccatcgttatttgtatcttgccatataaagtcacccggaacggcatcaggttgta tcactttaccgtcttttgatttatagttctgtatctgctcttcattttggaatattcctaagttcttataaaggcggaaataacccat agcatgaccttcctccatacgcgttacattaacagatgttctccagctaccaccatcagtatatccatttacatttcctatcttt acaacctcatttttaagatatgaggcatttgcggaaatagagaagttgatttcgttccaatttttattaaatgtcatctgcattt ccacaccctggtttgttatattaccaaggtttctaaaagctgcattattacctctaatggcttcaactgttggctggaacaac aaatccttagtactttttttaaaccagtcgaaacttgctctaatcataccattatagaatgtcatatcggcaccaacattaaa ttgttcagaagtttcccatttcacgtctggattaacaaggttattaggagcagatcccacagtgatggcattaccaaacgt gtaattataattattgccaataatagaagtataggagaatggagaaattcgctcatttccgttctgtccccaagagaatct aagtttgaagacatcaaagttcttaattttccagaatttctcatttgaaacattccaacctaatgaaacgcccgggaaagt agcatatctgttattgggaccgaaatttgaagacccatcgcgtctgaccacaacttccgccatatatttttcagcataatta tagcttagacgagcaaaatatgagaacatactatgtctaggattagcaccgccactattagctgatgtcataacatcac cagcattaagataccagtaattctcattggtcattgcttcatttggatatttatttcgtgttccggccataaactcataaacatc tcttgatgcagaagtacctaacaggacagatgtagaatgttcaccaaaagattttttatatcgcaatgtattctcccactgc caactactattagcatttgtactttgttctaccctagaattatcttctttacattctgcagaatgaaaaaactttggtgcaaaca ttcttccacggaaattccgatgattaataccaaaatctgtgcggaaaacaaggtctttaataaaagtgatctcagcataa acattaccaaaaaattgctgggtaatatttttattcttaggtgcctcatccataaatgcaatagggttccacatacggctata aggtacaggagagactccatatccgaaagtatcgttgctattctcatcataaaccggagtagtaggatcaatattatag gcgtatgatatcggattataaccattgataccggttgccactccactattctctatatatgcatagttgacgtttgcacctac acttaagaaatcatttatagaataggaactgttcagccttgtgctgaatcgtttgtaaaatgacgcatcttcaccgataata ccattctggtctagataattcaatgaaagcaagcttgaacccttatcactgccaaagttagcagtaatgttatgctcagta acaggagctgtattcaatatttcattaaaccagtctgtattataacctgttggagcagtaggtacaccaccggcaagcgg catatcatcattgtcggcaaactctttcatcagcataatgtactgttcatcattcagcatggttggtttctttgctactgtagag aaaccatagtaaccatcataagcaagcgatgtctttcctttctttcctttctttgtggttataaggactacaccattagcggct ctggcaccataaatagcagctgaagttgcatccttcaagacttccatgctttcaatgtcgttgggatttacactgttcatgtc gtccataggcagtccgtcaattacaaaaagaggattagagtttccatttgtaccaacaccacgaattaccagcttcggt gctgttcctggctgaccggaatttgtcacaacgttcacaccactaaccctaccgctcaatgcattcacggcatttgctggtt tagattgcaataaatcatcggaatcgatgctactgatagcacctgttacaacactttttttcttaacctcatatcctattgcta caacttcctcgagtgcaatggcagatgtttttaattgaacgtctatcttagactgacctttatacactatattctgtgtatcatat cctacgaagctataaatcaatgtcgattccattggtacattttccaagatataatttccgtccaaatcagaaataataccgt ttgtggtacctttaactaaaatacttgcacctatcacaggtaaaccatcggagtctgttatacaaccggtaactttcccgttc tgtgcatttaatggtaaactgaacgttataagaatcagcatacacattaatgatagtgttctgttcataatctagagttttttgt aattagtgtttttcttaaaataaaaagttttgttctatcagttgcgcgctacttactgacacttgcaaatatatatactatgtaat ataaccaaagggggaaaatttcatttaaataggggggggaaatagattaactaaatattttaaggaaaaatggctgtt agaatccattcccagactccaacagccattttatcactaacaatcgcctgttaatcaatatatttttctgcccatttccttaag atttgcatccctgcccagtggaacaaaagtaaatccgtatgaatagcttcccttcagaagacgcttgtctattgaaggac gggctttcagactccagctatctgttccgcccactccagcctgaaccaggtcgatattaagagtattagaatacaagtcc ttttcaagttcatttatatgtttagccttatcaatcgcattctgcgacatctcccacactgaaacagataggggttcatcgccg acaatcatcacacctgccttatccgactgcaaggcaaaccatctcacgtcacaacggtttccgttttcctgcggcattac atagtcaaatcccagagcggacaccttgcagttatatatagacaccattgcagaggcttttctgtcggaatagttttccca tgggccacgtccataatatgtcacatccgacaaacgattggtacattcgcattgcaatcctacgcgcaacatttctgatat ttcaggagacttcatcattgaataatgaacgcctattgttccgtctgcttttactttataattcaaggtaagtctcagtctttcat ctatagcctttagcaccttaacctcaagattgccttccgatttgcgtacatctatagaaactgtctttagctttaatggagcat ctttccagaatgcaaacagtctatcgaccttccatcctcgccagtcattgtctgttgacgctctccagaagtttggtttcaga gcagatgtgatgatactttcattatctatcttatactgactgatataaccatcactgatattcagataaaagttctttcccttca cgctgatgtctttcttgttatctgaatcgatttccatatccaatgtagtatcaacgcattctactatctttggtaaagaaagata cttaaactgttcccaggcaacctcgtatccagctttggcatacagattgtcattcttgagcctggcactcaggaataacca atattccgcaccgtcatcggccttgaaattctgaataggaagttttagtttacagctctcaccagctggtgttgtcggcaca ataatctcaccttcctgcaatacactgtcttcgtccttcaattgccaaaaataacgatactcatctgttgaaaggaagaag tttctgttttttacagttatctctccactatagacattatcagttgtaaatgatacaggagcaaacacgtacttgcattcctcag tagcaggtttaatggagcggtcggcactgataacaccatttatacagaagttttggtcgttgtgctcccctttctcatagtca ccaccataattccatgatttcttattatatttccgttcattatccagcaatccctggtctatccagtcccaaatatatccgccgg caagcgcatcatgagaacgtattgcatcccagtattctttcagcccgccggtagagtttcccatagaatgtgcatattcac acattattatcggacggttcatgaccggattcttagtcattgctataagctcatcgaccataggatacatacggctaatga catcgacgtataaaggatcatcgggattggcatacacacaaagctctttctttgccggtttgacatcttcgttcacattaaa atctatctcactagtaacgattgacgcttccttacgtccgataggtttgtataaaggattttccggctgtccttgcgccccctc gtaatgaacaggacgggttgggtcataatctttcagccatcctgacagagctgcatgattagggccgcatccagactc gttgcccaacgaccacataaacacagaaggatggttcctgtctctcacagccattcttaccactctctccatgaacgagt tagcccactcaggcctattggacagataccccctttgatgatgagtttcaagattagcctcatccattacgtatataccata cttatcgcacagttcatagaaataagggtcgttaggatagtgcgatgtacggactgtattgaagttataacgcttcataag cagaacgtcttcgagcatctcatcacgtgtaacggtcttacctccggtctcgctatggtcatggcggtttacaccaatgag tttaataggagtgtcattcaccagaatctgattacctgttattttaatatccctgaaccctaccttattacttctcgcatccacc acgttgccctttttgtctgtgagctttataaccaaagtgtatagataagggtgttccgaattccatagttttggcttagaaaca attccctccatcattccgtaataaacattatcacgctgaggataaggttcgttcaccacataatcggcagtaacggtaat gtcttttccaaacaccggtttcccatcggcatcatataattgggctgacagattccatcccttcaaatcatccatattctgatt tgttatttccggacggatctgtaaccgtgctatattcttccggaaatcgatgcgtgtccttactccataatcatatattgccac ctgcggaatggacatgatatatacttcacgatggataccagccattcgccagtggtcggcatcttccatataacttccgtc ggtccacttatacacttgcaccgccagtttattctcccccttcttaacgtattcggtaatatcaaattcagtaggcagacaa ctgtcttcggaatatcccaccttctgtccgtttatccatacattaaatcccgaatagacgcctccgaaatggagtataatcc tgtcgctcttccacttgtcaggaacaacaaactccttgatataacaccccgtctgattattcctgtcaatatatggcggacg agcagggaaaggataaatagtatttgtatatataggatagccatatccctgcatctcccaacatgaaggaacaggaat agttttccatgatgatgaattgtactccactttataaaaaccggcgggagccaatgccatatcctcggaaaagttaaactt ccattggccgttcaacgacatatactccgatttctctctgtctccatccaaagcccaatccactctccggaaagaataagt agtactgcgggaaggcaaacggttaattccgtttatggtctgatcctgccatacattctgattgtttctccactgattggcac cgttgtccgatgcagacagaaattgcatcatgaaaaataacacagaaaatgaaaaaatagattttaagttcaagttcat aaattcgcattttaagtttctatgcaaatatataagtataacgaacaatgaatagggggtatttctatctatatagagtggta tttttacatatgagctaaaacttaaaaaaaactgtcagtattactatgctatgtagcactctatatgaaaatattatatattcc caagtcaaaagccttttcaaacaatttttatatattctcatcctatcccttccatcaaagataaattccaatcctgatttgcca gccgcatttattccttttttcaggagaattttctttatggctatcgccatgaaaattcacctgaaaaagaatgcggcggcaa acggattagaattaaagaaaagattacagggattaactgcgaccgacgtgacgcatagccgtaattcaaaggcggc tatccttatattccatatatgacctcacaaatactgtgaaaatccactttccccaataacaaaacatagcctgccatatca acacccaaaa
[0230] SEQ ID NO: 74 (pZR1622 - vetor de resistência ao cloranfenicol):
ACTAGAGATCTATGTGAGGATCCTAACTCGAGATCGAGACTGCTATGAC TTGAGACCGGCTATTACGAGCGCTTAAACGGCGCGCCTGATAGGTGGGCTGCC CTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCC GGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGG TGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTC ACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAA CCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAAT CGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGATAAAACGAAAGG CTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTT GTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAA TGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATT TAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAAT AATTTATTTTCAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCA ATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTAT AACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATA AGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCA TCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGT TCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGG AGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTG GCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGT TTTTCGTTTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGC CAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAA GGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGA TGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTG GCAGGGCGGGGCGTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGG CCTTTTTTTTTGATAATAAGCGGATGAATGGCAGAAATTCGACGAGTCATTGGTA ACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATT TGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTG TGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATT GCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGT GACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTG ATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGA TGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTAT GGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGT GGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCC GAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCC AGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCC AAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATC GTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTG AGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGC AGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCAT CCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGA TGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCC CAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCA TGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAA ATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATT TCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTAT CTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTT GGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACA CCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAA TTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAA AAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAG TCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTC CGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACC CAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATA ATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTAT CCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAG TGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAA ACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTC TGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGG TATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCT CGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCG GTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGC CATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGA TTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAAC AGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTC ACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCA GTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGA AGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCAT TGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCC CATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATT TATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGA CGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCA GACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAG ATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAG GATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCA CGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACC CTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCA CGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATA GCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGC TTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAG CCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGA CATGGGGCGGCCGCTCAACGTACCGGTCTCAGTAGGGAGAGCTGTATGTGGGT AG
[0231] SEQ ID NO: 75 (pZR0879 - vetor de expressão Ec_frc, Of_oxc, Sc_YBR222C, Sc_YEL020C, Ec_yfdE, Of_oxlT (promotor de alta resistência)):
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGAC TGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTA ATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTG CAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATT ATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAAT GTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGC CCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAAC GTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATC GATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATA CCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGAT ATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGG GAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTG ATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAG CGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGC GCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTT GCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTC AATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCG TCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTT GGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCT GGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCAC TATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATG GATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGA TATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTG GCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAA TTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAAC CGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTAC GCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCA GGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCAT CTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGAC TGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAA AGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATG GTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATA TTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCC GGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCC ATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTG GCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAA GCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTT CTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAA GGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTAC CCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATT GATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATT GCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAAT AAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGT GACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTT CAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGA TGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATC ACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGT CACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAA CCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCC CTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGT GAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATG AATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACA TTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGT GGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAA GAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCT GCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCC AGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCC GCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAA GGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGC ACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGAT GCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGA TCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTG TTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTC CAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAAC AAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAA CTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATAT TTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGA CATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTAT TGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCG CTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCT GCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAA AGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCG TGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAA TACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACC ATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTAT CAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGAT TAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTG CTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTT TACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAAT TTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAA ACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGA AAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTA CTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTAT TGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGG TCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTC AGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCT TTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTAT AAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCG TCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCAC GTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGA GTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTG AACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTT CCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAA CGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACAC TTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTC CATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGG TTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTG TAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAA CCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTC CAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCT ATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAA AGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTA GATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGA GGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAAC TTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCT GAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATC GATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAA GCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAAC TTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTG ATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAA CTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTA GAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAAT CCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTA CAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTT AAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAAC AAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAA AGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTAC CCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATT GTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAA TTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGT TATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATC CACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTA ATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTG GATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTG ACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGC AGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTA GAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATAT GTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAA AAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAA ATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTA AGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAG CATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATT GCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGAT TCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCC ATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGC CAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATT CAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTG AATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAA TACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTAC AGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTG GGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGC TGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAG TAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACC ATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACT GCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACA CAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTA AAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAA TTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAA GGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTCTGGGTCTGACGGCCATCA AGGGTAGTGAGACTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTT GACTGGGTGTTCCGATACACTCAATTTGTGTAGGATAAAACGAAAGGCTCAGTC GAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCA ATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTT ATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAA TAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTAT TTTCAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGA TATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGC GCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTT GGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAG AGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATG CTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAAC TGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTT CATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATA CCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTC CGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTAT TCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCG CATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTG ATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCC TACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTT GTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAAC TGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTAT TCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTA GCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAG GCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGA ATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTT ATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTT CTCATCATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTT TTGACTTAGTTTCAATTTTACATTCCAGGAAGTAGGATAAAACGAAAGGCTCAGT CGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGC AATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCT TATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAA ATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTA TTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTG GTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATAT TCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCT GCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCT GGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTG GTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTG CTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTAT CGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCG GTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCA GCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGA TCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCA AGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAAT GCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTT GGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCT TGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTG GTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCAT GTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCT GCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGG GGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCA TACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATC TTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCT TTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTAT TCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAA AGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTT TTTTTTGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTG GTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTA GCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAAT AAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATC CTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTG GCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATA ATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGTCCATTT ATGTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGCAAAAC CATATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATACTCATA ATAAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAAAGAA GATATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAACTTCC TAAAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACCTACG TTTCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTGTTAG ATATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACATATTA ATACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAAGGT GCGTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATATTA TAAATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGAGAA TCTGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATGGTG ACACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTCGGG CTTCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACACTCC GGGACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGATGG AGCAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTTGCT GTTCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGATTG ACCGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATCTGT CTCAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGTTTG CTCCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGACGAC AATATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATTGGA ATACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATGGAT CAGCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTTTAT ACTTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAGAG CATGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCTGA GACTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAAAA TCTAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCCAA TGATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAGGT GCTGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATCCT CCGTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGAAT ACATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGATG AATTGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGCT GGAAGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAAA GAACGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAAC CCTTATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGGG TTGCAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAATG CCGTTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAAG TGACTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTAC ACCAGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAACA GTCAGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCCA AGCGGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGAA GATATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAATA CAAGTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTTT TATGGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATATC CGCATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAAA ATAACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGA TTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTT CATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGAT TCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAG CAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTT TCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCG TTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATG TCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGC TGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTC TTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGA AAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTT CAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTC AGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGC TCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTC TCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTT GAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCT GCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTA CAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTT CACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGG CATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTAT TTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATC CGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGC CCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTA CGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGAT GTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACA CCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTG TAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATA AGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAA CAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAA GATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAG AAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTC CTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAA CTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCAT ATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCA TAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAAT ACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCA TGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAG ACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACC AAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCC AGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATG CTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGG ATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTG ACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACA ACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCC CGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATT TAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCT TGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATC TTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAAT CGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTAC GTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTC TCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAAC GTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAA TATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAG AATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAG GGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACA CAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
[0232] SEQ ID NO: 76 (pZR0901 - vetor de expressão Ec_frc, Of_oxc,
Sc_YBR222C, Sc_YEL020C, At_AAE3, Ec_yfdE, Of_oxlT (promotor de média resistência)):
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGAC TGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTA ATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTG CAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATT ATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAAT GTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGC CCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAAC GTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATC GATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATA CCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGAT ATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGG GAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTG ATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAG CGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGC GCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTT GCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTC AATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCG TCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTT GGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCT GGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCAC TATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATG GATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGA TATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTG GCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAA TTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAAC CGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTAC GCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCA GGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCAT CTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGAC TGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAA AGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATG GTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATA TTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCC GGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCC ATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTG GCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAA GCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTT CTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAA GGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTAC CCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATT GATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATT GCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAAT AAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGT GACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTT CAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGA TGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATC ACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGT CACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAA CCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCC CTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGT GAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATG AATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACA TTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGT GGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAA GAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCT GCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCC AGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCC GCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAA GGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGC ACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGAT GCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGA TCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTG TTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTC CAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAAC AAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAA CTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATAT TTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGA CATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTAT TGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCG CTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCT GCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAA AGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCG TGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAA TACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACC ATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTAT CAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGAT TAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTG CTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTT TACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAAT TTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAA ACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGA AAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTA CTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTAT TGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGG TCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTC AGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCT TTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTAT AAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCG TCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCAC GTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGA GTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTG AACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTT CCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAA CGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACAC TTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTC CATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGG TTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTG TAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAA CCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTC CAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCT ATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAA AGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTA GATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGA GGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAAC TTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCT GAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATC GATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAA GCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAAC TTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTG ATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAA CTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTA GAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAAT CCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTA CAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTT AAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAAC AAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAA AGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTAC CCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATT GTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAA TTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGT TATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATC CACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTA ATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTG GATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTG ACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGC AGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTA GAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATAT GTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAA AAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAA ATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTA AGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAG CATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATT GCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGAT TCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCC ATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGC CAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATT CAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTG AATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAA TACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTAC AGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTG GGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGC TGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAG TAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACC ATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACT GCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACA CAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTA AAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAA TTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAA GGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTCTGGGTCTGACGGCCATCA AGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAA TTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAA GTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAA GTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGA GATTCAAAGGTAATATTAACAATAATTTATTTTCAATGGATAGCGATACTCTCTCA GGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTT TCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCC GCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTC ACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTA GAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTT TACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCAC CGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGC TTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTG ACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACA CTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAG CTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTAC GGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGC TCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCA ACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGC CGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAAT ATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATT GTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGA CAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACA AGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAG AAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCA AATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAG TTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGC ATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTC CAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTG CATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTC CAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGA ATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCA AAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGA AGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTT TGACTGGGTGTTCCGATACACTCAATTTGTGTAGGATAAAACGAAAGGCTCAGT CGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGC AATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCT TATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAA ATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTA TTTTCAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCG ATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGG CGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATT TGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAA GAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATAT GCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAA ACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGC TTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGA TACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGC TCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTT ATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGG CGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTC TGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATC CCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTG TTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGA ACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCT ATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGT TAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTG AGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGG GAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCAT GTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGA GTTCTCATCATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTT TTTTTTGACTTAGTTTCAATTTTACATTCCAGGAAGTAGGATAAAACGAAAGGCTC AGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTT TGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGT TCTTATATTTCCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAA AAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAAT TTATTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCG TTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAA TATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTG GCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAG CCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCG GTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTC CTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACG GTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCT TCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACT GATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGG TTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGC CAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGC GAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTA ACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCG GTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATG GTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAA CCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGAT TGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCAC ATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTAC GGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTC GATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATAC CGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTC GTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAA GAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATG GCCTTTTTTTTGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCT TCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGG CGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTG CGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCAC CTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACC CTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCG CTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGT CCATTTATGTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGC AAAACCATATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATAC TCATAATAAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAA AGAAGATATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAAC TTCCTAAAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACC TACGTTTCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTG TTAGATATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACAT ATTAATACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAA GGTGCGTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATA TTATAAATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGA GAATCTGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATG GTGACACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTC GGGCTTCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACAC TCCGGGACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGA TGGAGCAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTT GCTGTTCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGA TTGACCGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATC TGTCTCAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGT TTGCTCCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGAC GACAATATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATT GGAATACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATG GATCAGCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTT TATACTTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAG AGCATGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCT GAGACTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAA AATCTAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCC AATGATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAG GTGCTGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATC CTCCGTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGA ATACATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGAT GAATTGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGC TGGAAGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAA AGAACGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAA CCCTTATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGG GTTGCAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAAT GCCGTTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAA GTGACTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTA CACCAGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAAC AGTCAGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCC AAGCGGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGA AGATATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAAT ACAAGTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTT TTATGGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATAT CCGCATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAA AATAACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTG ATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTT TCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGA TTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGA GCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGT TTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATC GTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGAT GTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGG CTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTT CTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGG AAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTT TCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTT CAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAG CTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTT CTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGT TGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGC TGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTT ACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCT TCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTG GCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTA TTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACAT CCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAG CCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGT ACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGA TGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATAC ACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATT GTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTAT AAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAA ACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTA AGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGA GAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGT CCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAA ACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCA TATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCC ATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAA TACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACC ATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCA GACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAAC CAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCT GTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGC CAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAAT GCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACG GATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCT GACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAAC AACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGC CCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAAT TTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCC TTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTAT CTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAA TCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTA CGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCT CTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAA CGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTA ATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAA GAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCA GGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGAC ACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
[0233] SEQ ID NO: 77 (pZR1871 - vetor de expressão Ec_frc, Of_oxc,
Sc_YBR222C, Sc_YEL020C, At_AAE3, Ec_yfdE, Of_oxlT (promotor de alta resistência)):
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGAC TGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTA ATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTG CAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATT ATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAAT GTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGC CCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAAC GTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATC GATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATA CCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGAT ATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGG GAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTG ATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAG CGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGC GCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTT GCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTC AATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCG TCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTT GGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCT GGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCAC TATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATG GATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGA TATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTG GCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAA TTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAAC CGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTAC GCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCA GGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCAT CTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGAC TGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAA AGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATG GTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATA TTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCC GGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCC ATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTG GCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAA GCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTT CTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAA GGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTAC CCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATT GATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATT GCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAAT AAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGT GACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTT CAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGA TGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATC ACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGT CACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAA CCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCC CTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGT GAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATG AATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACA TTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGT GGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAA GAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCT GCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCC AGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCC GCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAA GGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGC ACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGAT GCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGA TCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTG TTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTC CAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAAC AAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAA CTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATAT TTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGA CATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTAT TGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCG CTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCT GCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAA AGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCG TGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAA TACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACC ATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTAT CAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGAT TAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTG CTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTT TACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAAT TTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAA ACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGA AAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTA CTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTAT TGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGG TCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTC AGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCT TTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTAT AAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCG TCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCAC GTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGA GTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTG AACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTT CCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAA CGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACAC TTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTC CATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGG TTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTG TAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAA CCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTC CAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCT ATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAA AGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTA GATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGA GGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAAC TTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCT GAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATC GATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAA GCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAAC TTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTG ATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAA CTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTA GAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAAT CCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTA CAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTT AAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAAC AAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAA AGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTAC CCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATT GTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAA TTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGT TATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATC CACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTA ATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTG GATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTG ACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGC AGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTA GAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATAT GTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAA AAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAA ATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTA AGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAG CATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATT GCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGAT TCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCC ATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGC CAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATT CAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTG AATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAA TACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTAC AGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTG GGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGC TGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAG TAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACC ATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACT GCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACA CAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTA AAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAA TTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAA GGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTCTGGGTCTGACGGCCATCA AGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAA TTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAA GTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAA GTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGA GATTCAAAGGTAATATTAACAATAATTTATTTTCAATGGATAGCGATACTCTCTCA GGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTT TCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCC GCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTC ACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTA GAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTT TACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCAC CGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGC TTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTG ACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACA CTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAG CTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTAC GGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGC TCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCA ACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGC CGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAAT ATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATT GTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGA CAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACA AGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAG AAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCA AATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAG TTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGC ATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTC CAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTG CATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTC CAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGA ATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCA AAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGA AGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTT TGACTGGGTGTTCCGATACACTCAATTTGTGTAGGATAAAACGAAAGGCTCAGT CGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGC AATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCT TATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAA ATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTA TTTTCAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCG ATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGG CGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATT TGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAA GAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATAT GCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAA ACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGC TTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGA TACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGC TCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTT ATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGG CGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTC TGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATC CCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTG TTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGA ACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCT ATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGT TAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTG AGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGG GAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCAT GTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGA GTTCTCATCATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTT TTTTTTGACTTAGTTTCAATTTTACATTCCAGGAAGTAGGATAAAACGAAAGGCTC AGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTT TGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGT TCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAA AAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAAT TTATTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCG TTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAA TATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTG GCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAG CCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCG GTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTC CTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACG GTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCT TCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACT GATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGG TTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGC CAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGC GAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTA ACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCG GTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATG GTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAA CCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGAT TGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCAC ATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTAC GGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTC GATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATAC CGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTC GTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAA GAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATG GCCTTTTTTTTGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCT TCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGG CGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTG CGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCAC CTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACC CTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCG CTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGT CCATTTATGTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGC AAAACCATATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATAC TCATAATAAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAA AGAAGATATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAAC TTCCTAAAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACC TACGTTTCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTG TTAGATATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACAT ATTAATACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAA GGTGCGTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATA TTATAAATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGA GAATCTGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATG GTGACACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTC GGGCTTCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACAC TCCGGGACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGA TGGAGCAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTT GCTGTTCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGA TTGACCGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATC TGTCTCAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGT TTGCTCCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGAC GACAATATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATT GGAATACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATG GATCAGCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTT TATACTTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAG AGCATGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCT GAGACTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAA AATCTAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCC AATGATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAG GTGCTGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATC CTCCGTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGA ATACATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGAT GAATTGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGC TGGAAGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAA AGAACGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAA CCCTTATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGG GTTGCAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAAT GCCGTTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAA GTGACTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTA CACCAGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAAC AGTCAGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCC AAGCGGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGA AGATATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAAT ACAAGTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTT TTATGGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATAT CCGCATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAA AATAACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTG ATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTT TCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGA TTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGA GCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGT TTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATC GTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGAT GTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGG CTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTT CTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGG AAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTT TCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTT CAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAG CTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTT CTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGT TGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGC TGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTT ACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCT TCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTG GCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTA TTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACAT CCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAG CCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGT ACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGA TGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATAC ACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATT GTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTAT AAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAA ACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTA AGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGA GAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGT CCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAA ACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCA TATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCC ATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAA TACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACC ATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCA GACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAAC CAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCT GTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGC CAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAAT GCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACG GATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCT GACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAAC AACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGC CCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAAT TTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCC TTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTAT CTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAA TCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTA CGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCT CTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAA CGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTA ATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAA GAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCA GGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGAC ACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
[0234] SEQ ID NO: 78 (RBS e peptídeo líder N-terminal):
GATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGAT TCAAAGGTAATATTAACAATAATTTATTTTCA
[0235] SEQ ID NO: 79 (RBS e repórter de luciferase NanoLuc N-terminal):
TCCATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTT GGCGATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGG TGGCGTAAGCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGC GCATCGTTCTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCC CGTACGAAGGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAG TCGTCTACCCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCT GGTGATTGATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGA AGGCATTGCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAA TGGCAATAAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTT CCGCGTGACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGG CA
[0236] SEQ ID NO: 80 (P_BfP2E5):
GATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATT GGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGT TTCACTTGAACTTTCAAATAATGTTCTTATATTTCCAGTGTCGAAAGAAACAAAGT AG
[0237] SEQ ID NO: 81 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGC AGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACG AGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGG ACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGT ATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTA AAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAA GTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTG AAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATA AGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAA GAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTT GCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATT TTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAG CGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATT GGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGA TTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGC AGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGC TATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAG TATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACA GCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGG TATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTC TCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCA TTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTC AATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTG CTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCC CTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGAT ATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGC TGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAG TCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTG AAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTT TACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAAT TTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAA ACAAAGTAGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAA AAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACC GGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGAC GTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGA GATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTT CTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGA TCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACA TGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTC GATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAAC GTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCC GCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGC TGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAAC GAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTAC GTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATC CGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGC GGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGC CTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATC GGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACA GCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATA AACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTT AAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTT GAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAA TTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACC GCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAG CAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGGCATCAACTACCTAAATTAA TTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATG TAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACAT CGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTG GCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTA TGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGT TTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAA CTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTT GCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTG CAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGT GCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCA GCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCA AACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCG CTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCG CTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGC ATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCA CAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTA CTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTG GGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGG ACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTT CCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACC GGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGAT GACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGT TCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAA TGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCT TGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGC AGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCG GTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCG TTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACC AGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGA AGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGC TCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGA TCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAA AGTTGGCAAGAAATAAGGTTCCTAAACGACCTGGCATCACCTAAAAATTATAAAT AAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGA CAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGC TGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGG TTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCT CTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATT CGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGAC CTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCC GGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATG GTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTC TGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTG TTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGAT CGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGT TACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGT TCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAAC AGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGG TGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTT TCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTG TTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCG CCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTT CTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAA TTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTT CATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAA GAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTC CCCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATT TAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCG ATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGG CGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATT TGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAA GAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATAT GCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAA ACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGC TTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGA TACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGC TCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTT ATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGG CGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTC TGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATC CCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTG TTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGA ACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCT ATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGT TAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTG AGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGG GAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCAT GTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGA GTTCTCATCATAAGGTTCCTAAAAACCCTGGCATCATCTACCTATATTAATCATCA ACCCAATTTTCATATGTATAAAATTAACAAAATGACAAGTGCCGCTACTGTTACTG CTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGA AACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCA GACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGAT ACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGT GCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAG GAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGG GTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATG TTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATAT ATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGC CAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGAC GTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCT TTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGC TAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTT AATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGAT GGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGT TCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACC CTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAAC GTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAA GCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACA ATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAA AACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCA AGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGT TTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGA TTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAG CCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCA TTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAA GAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTA CCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCT AAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCA TCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAAT AATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCG GTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCAC TACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCA AGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGA GTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGA CTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAG GCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAA TCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTAT CAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCT AGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAA CGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAG TATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAA GATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCC ATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGC TTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTG GTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGC CCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCG GAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTG GTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCT GGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAAC GTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATT GTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGT TTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTG CCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCAT TTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCA GACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAA CATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTC GCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTT CATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCT CGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAA GAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGAC GCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTAT ATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGC AGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAA GGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTAT CATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAAT TTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCAT ATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTT TCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAA AGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACA GACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATA AATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAA TATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAA TTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGT CATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTG ATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAA ATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAAT ATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTG AAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTA TTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTA TATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCAC ATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAA TATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAA AAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATT AGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAA ACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTG GAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAG CATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAA AAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGG CAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTA TTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGAC GACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCA ACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTA TTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGA CAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATC AACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCC CGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGC TTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCA TCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCG CATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTAT CGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGG CCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACT GTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAA TGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTT TGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCA TCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCG TGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTG ATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATAC CATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCC AGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGA TATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGG ATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCA AGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAA ACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGAT GATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGT ATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCAT ATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGT CATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATAT CAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAG GATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAA ACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGT CTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTA AGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTC TTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTA GCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGC TGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAG GCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGT GAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTA GCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCT ACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATG GCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCA GAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATAC CCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAG TACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTT ACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGC ACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGC ACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGA TACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGA TAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGA GGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAAT ACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGG ATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTC CGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTT CTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAAC GCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAG CGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACT TCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGG CTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGA GGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTT TAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCT CACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTT TCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAA TTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTA AAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCT GTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCAC GTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCC TCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCAC GTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAG CCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCT TTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGC CCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGAC ATGGGGCGGCCGCGTAG
[0238] SEQ ID NO: 82 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAA AATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATC TGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAA TTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCC TGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGA GTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGC GAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGG CTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGAT CAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACG TTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCC GCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTG CTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGC AACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAA TTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCA GTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGT GGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATC CTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTA AAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCA TGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGT CACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTG TGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAG TGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTG GTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTA TTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATG AAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGG CATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAA AATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCG ATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCT ATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTT CCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAA GGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCG TGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGC GGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAAT GGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACA GCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCC GGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGA CCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCT CCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCA AGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACA ATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTG CCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTG CTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGG CGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGAC GAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAA CCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTG CTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCG CTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGAC TGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTC GTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAAT GCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCT TGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCT GCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCAT TCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTT ACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCC ACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGA TGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTG AAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACG CGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAA CGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAACGACCTGGCATCACC TAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATA ATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTG GTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGAC ACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTC AGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGT GGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGC GATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTC TGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATC GCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCG GTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGAT CAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTT TGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCC AGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGC GAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGT TAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGC CTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGT TCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGT TACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTC CCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCG CATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGAT ATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAA AGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAAC TTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTT GCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGT CAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTCCCCTGGC ATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATTTAAAAAA TGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATG ACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCA AGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTG GTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAG AGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATAT GCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAA AACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATAT GCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTA CGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTG ATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGT CTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGA GAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGA GCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGA AATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCC GATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACT GGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCG TACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAG GCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGC CGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAAT ATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAG CGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCA TGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAAACCCTGGC ATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAATTAACAAA ATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCC GATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGA TCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCC ATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATG GGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGC GCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGAC CTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTC TGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTT CGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACT ACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATC CCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCAT ACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACAT TGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACA GATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTAC TTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAA AGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTC CCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACAC ATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAG CTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAG CATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGT ACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACA ATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGT CACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGA GAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTT TTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGAT TTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAG CCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGC CATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCT TAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATA AGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACT TTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAG AAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGG TGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATT CAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGG CTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAA CCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGA TAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTG AAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCC GGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGT AATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCAT CGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTA CGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATC CCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGC TGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTT AGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACC CTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCC TTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAAT TAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGA AAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCAC ATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTG TGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCT GCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGT GCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGG AATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTG GCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGT GTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGA AACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTT GTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGG AGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACT TTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGC GCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATC CGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCA GGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAAC ACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTG GATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTG CGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGT TATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTG TTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCAT TTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTT TAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATA TTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTT TTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCA GAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATA TACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAA AACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACA AAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAA AATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGC AGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGG CGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATT ATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCA CAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATT CGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATAT GGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATG GCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCA AAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATG GCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAA GAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCG AGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATA GTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTT TACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAAC CAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCAT ATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGA AAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCG ATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATA AGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATG GCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGC TCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCC TGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAAT CGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCT GAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGT GGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAA GAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCAT TGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTC CCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCA TTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCA AGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTA AGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACAT CAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGA GTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGT CAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTA TCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATA CGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACC ATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCC AGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTG ATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGAC GGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGC TTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGG AAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGG ACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACA GAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGT GTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAAC ACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACG GGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTAC CGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTG TCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTT TTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTA CGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATG CGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTT TCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTA AACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAA AGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATAT AAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACC ATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGT CTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTT CGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTC GAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCA CCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCG CTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATA CCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTT ACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACG GGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTG GCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTAC ATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTT CGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGT TTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCG GGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTC GGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTT CGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACA ATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCA AATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGT TGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTT TTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTC CCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAG GCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTT TAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCC TCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTT GTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACT TTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCC AATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAG GATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCT CATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAAC GAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGC TCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATC AGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATAT AAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATG TAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGG AACACTTAACGGCTGACATGGGGCGGCCGCGTAG
[0239] SEQ ID NO: 83 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAA AATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAA TTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACT ATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAG TTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTA ATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTAT GTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGAC ATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTT CCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAA AAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTC CCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTG GTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAG TAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATT AATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGG TTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTG CCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGA TTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTC GGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAA CCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCT GGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACA GGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATA CAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGC TTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCA CGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTT CCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGG GTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGAC GTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGA GACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTG GGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTT GTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACT TCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAAT TGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGG AGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGT TCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGA AAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTT CACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGC GCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCA GCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAA CAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGG AAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCC ATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCT GATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAA AGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGG TTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAAC ACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAA AACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAAC CTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCT GGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGC GGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAG GCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACT GGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGC ATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAAT CGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTC AGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATC CCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGG AAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATAT TAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTG GGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAG GTTCCTAAACGACCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTA ATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGC TTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTT CTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTG ATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGT TTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGAT AAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTC GCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATC AACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCT AAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGA TGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGC GGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATG GCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGC CACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGT CACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTC AATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCT GCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAG CAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCG GACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTG GTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAA ACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTA ATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGG CAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGG TTCCTAAGGGTCCTGGCATCAACTACCTACATTAATAACCAACCCAAATGCCTA ATCAATAAATATTATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTC TTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATC TCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCT TGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTC AAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGA ATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGG GTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCA GGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCC GGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGT GTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGG CGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCG CTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTT ACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGT TCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCA ACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGAT CGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTT TCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATC AATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGC CTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTC TGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAAC TATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCT GGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTG CGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGG GCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT TAAGGTTCCTAATTCCCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCA ACAATTTAAAAAAAATTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGA TGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTA TGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGAC GGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTC TATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCC CTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTT CCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAAC AGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAA GCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGC AGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCA GCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTT CAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTC GCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAA AGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAA ACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACA ACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGA CGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAA GGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGG CTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATC AAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTG ACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAA ACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCC AATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCT GGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGC TGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGT ATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTG TTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGC CGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAA GGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCC GTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCC AATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAA ACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGC CGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTA AAATGCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAA AAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCC AAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACT CACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCG ACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTC GAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCC GTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAG ATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCA GCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTC GGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACG GAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCAC TACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCC GTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATT GTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTT GGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAAC ATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGA CCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATAT CCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATT GTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATG ACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCAC ACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAA TGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGA GGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAG GTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGAT GGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTG AGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTT TCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAA CTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAA GCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCAT CACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCG CACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAAACCCTGGCATCTTTTC CATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAG TGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGT CGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCC ACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTAC GGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGG AATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCC TTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAA TCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAAT TCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATG CGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAA AGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTT AAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAG TGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGA GAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCT TATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCC ACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTA TTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACA ATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGA TTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGA GAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCA CATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGT GGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTC TTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTT TGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAG AACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGT CCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCAC CCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTT GCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGT TTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGA AGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTAC CAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCT AAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCC ATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGA ATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCA TCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTG CCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACT CCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGA TGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAA TGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACT TTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCC ACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTG GTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTA GAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCG GTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATG TAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTG GCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCC CGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACT TTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATA ATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTG GTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGC GATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATT CTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTG TTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCT CGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGG CATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTT CATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCAT GATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATT CGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTT CCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAAT AAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTA GTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCC TGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTG CCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTC CCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCG CTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATAC ACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAA AAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGC AGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCT CTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTAT CGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGC ATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTT TGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCC GACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAA GATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACA GACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACA TAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGT AAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATA ATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGG AAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATA GAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTA ACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCC ATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAA AATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTT GCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAG GTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTA AAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTA AAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACG AAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATA TGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAA ATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGG GTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTG ATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGAT TATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCAC CGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGAC TCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATC AAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGC TTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCA AAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAG GCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCA ACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGG ATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTA ACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATA AATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAAC GCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACA ATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATAC CCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGT TTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGA CAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGA TTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAG GATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGAT GGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTT CAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCG ATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGAT TCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGG TGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCC ATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCT TTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAG TCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAA CAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGAT GATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAA GTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTG CATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGC CAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAA GATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTT CTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGA CTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTT CATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAAT CTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGA GCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAG TTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAA TTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACAT CCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACA TTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAA ACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGA CTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAA AAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTT TTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATAT TTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATAT TTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACC GAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCG CAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTA AGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGA GCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGC GCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACG ATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATC ACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAG CTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAG TTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAAT GCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGA GCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAG TCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTT GTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTG TTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTT ACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTA AGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTT TTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGA CTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTT CGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTT AACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAA ACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTG TACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCAC GTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACC CTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTC ACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAA TAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAA AGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGA GAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACG GCTGACATGGGGCGGCCGCGTAG
[0240] SEQ ID NO: 84 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAA AATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAA TTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACT ATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAG TTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTA ATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTAT GTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGAC ATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTT CCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAA AAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTC CCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTG GTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAG TAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATT AATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGG TTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTG CCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGA TTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTC GGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAA CCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCT GGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACA GGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATA CAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGC TTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCA CGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTT CCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGG GTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGAC GTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGA GACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTG GGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTT GTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACT TCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAAT TGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGG AGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGT TCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGA AAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTT CACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGC GCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCA GCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAA CAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGG AAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCC ATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCT GATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAA AGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGG TTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAAC ACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAA AACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAAC CTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCT GGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGC GGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAG GCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACT GGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGC ATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAAT CGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTC AGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATC CCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGG AAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATAT TAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTG GGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAG GTTCCTAAACGACCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTA ATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGC TTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTT CTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTG ATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGT TTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGAT AAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTC GCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATC AACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCT AAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGA TGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGC GGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATG GCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGC CACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGT CACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTC AATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCT GCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAG CAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCG GACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTG GTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAA ACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTA ATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGG CAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGG TTCCTAAGGGTCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACCTTTTA ACATAAAAATTAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCT TGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCT CGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTT GGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCA AGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAA TTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGT ATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGG TGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGG ACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGT TCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCG CAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTT TCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACC GACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCT GTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACA GCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCG GTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTC GTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAAT GCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTT TATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGT TCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTAT GGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGG GTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCG ATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGC CGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTA AGGTTCCTAATTCCCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAAC AATTTAAAAAAAATTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATG GCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATG GTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGG TCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTA TCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCC TGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTC CCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACA GGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAA GCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGC AGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCA GCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTT CAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTC GCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAA AGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAA ACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACA ACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGA CGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAA GGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGG CTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATC AAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTG ACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAA ACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCC AATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCT GGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGC TGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGT ATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTG TTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGC CGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAA GGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCC GTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCC AATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAA ACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGC CGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTA AAATGCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAA AAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCC AAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACT CACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCG ACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTC GAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCC GTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAG ATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCA GCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTC GGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACG GAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCAC TACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCC GTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATT GTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTT GGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAAC ATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGA CCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATAT CCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATT GTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATG ACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCAC ACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAA TGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGA GGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAG GTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGAT GGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTG AGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTT TCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAA CTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAA GCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCAT CACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCG CACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAAACCCTGGCATCTTTTC CATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAG TGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGT CGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCC ACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTAC GGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGG AATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCC TTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAA TCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAAT TCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATG CGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAA AGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTT AAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAG TGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGA GAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCT TATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCC ACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTA TTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACA ATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGA TTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGA GAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCA CATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGT GGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTC TTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTT TGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAG AACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGT CCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCAC CCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTT GCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGT TTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGA AGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTAC CAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCT AAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCC ATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGA ATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCA TCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTG CCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACT CCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGA TGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAA TGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACT TTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCC ACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTG GTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTA GAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCG GTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATG TAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTG GCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCC CGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACT TTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATA ATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTG GTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGC GATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATT CTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTG TTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCT CGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGG CATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTT CATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCAT GATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATT CGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTT CCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAAT AAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTA GTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCC TGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTG CCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTC CCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCG CTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATAC ACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAA AAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGC AGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCT CTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTAT CGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGC ATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTT TGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCC GACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAA GATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACA GACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACA TAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGT AAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATA ATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGG AAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATA GAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTA ACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCC ATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAA AATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTT GCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAG GTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTA AAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTA AAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACG AAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATA TGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAA ATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGG GTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTG ATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGAT TATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCAC CGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGAC TCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATC AAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGC TTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCA AAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAG GCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCA ACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGG ATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTA ACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATA AATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAAC GCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACA ATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATAC CCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGT TTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGA CAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGA TTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAG GATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGAT GGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTT CAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCG ATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGAT TCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGG TGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCC ATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCT TTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAG TCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAA CAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGAT GATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAA GTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTG CATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGC CAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAA GATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTT CTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGA CTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTT CATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAAT CTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGA GCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAG TTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAA TTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACAT CCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACA TTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAA ACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGA CTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAA AAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTT TTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATAT TTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATAT TTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACC GAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCG CAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTA AGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGA GCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGC GCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACG ATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATC ACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAG CTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAG TTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAAT GCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGA GCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAG TCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTT GTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTG TTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTT ACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTA AGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTT TTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGA CTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTT CGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTT AACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAA ACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTG TACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCAC GTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACC CTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTC ACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAA TAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAA AGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGA GAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACG GCTGACATGGGGCGGCCGCGTAG
[0241] SEQ ID NO: 85 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCACCTAAAAATTATAAATAAATTAATAAACCACAATACTAAAAATTTAATAA AATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGT TCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATT CCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCT GCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCC TGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCG GTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGT TCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTT ACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCT GGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTG CCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTA CACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTT CCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCA ATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTC TTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGG TCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAG AACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAAT CGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCG CACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTG GCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAA CAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGG CTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGC AACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCAT CGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGA AAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTG CCTGGCATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAATT AACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGC GTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTA CAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTA ATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATG CGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAA AATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTT GAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTAC AGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGC TGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAG GACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAAC ACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGAT TTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTT GAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCT TGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGT GTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTC CATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGT TGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTT CCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTT CCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATG ACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAA GCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGAC AATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCG AAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTC ACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGA GGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAA CGACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAA AATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAA GTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGG CCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAA GCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTG TCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTAT AATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCC AGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCT AAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGA TAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTAT ATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATG ATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCA GACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAA GAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTA GACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAA GGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAG GCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGAT CCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATT TGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGAT TTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGC TCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAG GGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTG GAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGAC GACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTA GAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAAT GCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCC TGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTA TGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATG AATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCC GACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAG CTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGG CAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATC GAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGAC TGTAAGGTTCCTAAGGGTCCTGGCATCTAAACTAAAATACAATAACCAAAAATC ATGTTTAACAAATAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATT GGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAA AATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCA CGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCT TCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGA GCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTT TACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCA CCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCT GCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTC GTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCC TCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCT CAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTC TGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGG GTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTA GATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGG TATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCA CCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTT TGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCT CGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTAC CAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGA AATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGA GAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGA AGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGT TACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTAT TAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAA CGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATAT GGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCG AAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCA AAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCA GCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAATTCC CCTGGCATCATCTACCTATATTACTAAACAAACCAATTATAATAATTTTAAAAAT AAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATC GATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATG GGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCA CATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATG GCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTA TAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAA TGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTC ATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCC TGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGAT ATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGG CGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGA GCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTT TCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGT CTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGA TAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCA GGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTT TACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAA CGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGT CGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAATG CCTGGCATCATCTACCTAAATTAATAAACAACCCATTTATAAACCCTTTAAAATT AACAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTG CAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTAT TAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGAT ATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCT ATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGAT CCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACA TGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGT TCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGA AAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGAT GGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATG CATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCG TGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGC GTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATA CCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAAT GCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAAAA CCCTGGCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATA TAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTT GATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCA TTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTAC AGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACAT CGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAAC GGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTT GAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAA GAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTAT CAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTG TATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGT CAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCC AGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTG ATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACG CACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATT CCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCC GCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGA TCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGG CGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACA GCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTC CCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACC GGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGA TGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTT GTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGC TAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAAC GTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTT GCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCG CATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCA GTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGA TCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACA TGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAA CTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAA CGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTG AACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGATTAGAAGGCC ATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGA ATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCA TCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTG CCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACT CCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGA TGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAA TGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACT TTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCC ACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTG GTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTA GAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCG GTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATG TAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTG GCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCC CGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACT TTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATA ATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTG GTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGC GATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATT CTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTG TTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCT CGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGG CATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTT CATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCAT GATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATT CGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTT CCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAAT AAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTA GTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCC TGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTG CCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTC CCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCG CTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATAC ACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAA AAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGC AGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCT CTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTAT CGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGC ATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTT TGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCC GACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAA GATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACA GACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACA TAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGT AAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATA ATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGG AAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATA GAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTA ACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCC ATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAA AATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTT GCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAG GTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTA AAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTA AAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACG AAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATA TGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAA ATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGG GTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTG ATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGAT TATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCAC CGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGAC TCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATC AAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGC TTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCA AAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAG GCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCA ACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGG ATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTA ACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATA AATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAAC GCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACA ATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATAC CCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGT TTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGA CAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGA TTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAG GATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGAT GGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTT CAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCG ATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGAT TCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGG TGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCC ATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCT TTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAG TCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAA CAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGAT GATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAA GTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTG CATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGC CAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAA GATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTT CTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGA CTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTT CATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAAT CTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGA GCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAG TTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAA TTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACAT CCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACA TTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAA ACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGA CTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAA AAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTT TTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATAT TTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATAT TTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACC GAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCG CAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTA AGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGA GCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGC GCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACG ATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATC ACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAG CTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAG TTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAAT GCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGA GCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAG TCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTT GTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTG TTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTT ACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTA AGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTT TTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGA CTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTT CGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTT AACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAA ACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTG TACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCAC GTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACC CTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTC ACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAA TAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAA AGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGA GAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACG GCTGACATGGGGCGGCCGCGTAG
[0242] SEQ ID NO: 86 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAA ATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTT CTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTC CTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTG CGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCT GGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGG TGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTT CCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTA CGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTG GCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGC CACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTAC ACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTC CCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAA TTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCT TTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGT CGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGA ACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATC GGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGC ACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGG CAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAAC AGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGC TGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCA ACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATC GCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAA AATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTG CCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAAT AATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTG CAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTAT TAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGAT ATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCT ATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGAT CCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACA TGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGT TCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGA AAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGAT GGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATG CATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCG TGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGC GTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATA CCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAAT GCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACG ACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAA TAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG TAAGGTTCCTAAGGGTCCTGGCATCAACTACCTAAATTACTTATCAAACCACTT AGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTG AAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTC AACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCAT GGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTA CAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCA CGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAA TTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAG AAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGT CCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGAT AATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTC TTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTT TATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTG ATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGG AAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGT TTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTT TTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGAT TTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTG AGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGA AGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGC CACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCC GGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGA GCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCAT AAGGTTCCTAATTCCCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCA TATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTT CAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGG ACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGAC CATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATAC AGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTG CTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAG GAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAG GGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGG ATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATG ATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACA ACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCC AGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAAC GGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACA CTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCAC GTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTT GTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTA CAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACAT GCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGC TTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCT TGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTG CCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACC GTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCG AAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAA GCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCA AGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGC TTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATG CTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATAT GTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGA CCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTC CAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATC CAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAA GTTGTAGGGTTCCTAAAATGCCTGGCATCAACTACCTAAATTAATTATCACCCC AAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTG ACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACC ATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGA TGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAG CTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCC GCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACT GCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTG CAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACT GCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCT CGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACT TGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTG CTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTAT CGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTG GGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTG AAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCC TGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAG TGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACG GTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACAT CCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGT GACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAA AAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAA AGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAAC TACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATAT TTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTG ACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGG TATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTA TCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAAC CATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTA TCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGT CTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTT ATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCT TCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCG AATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAA GGTTCCTAAAAACCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATTAC AAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAA ACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTC AATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCT TGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTA ACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACG GCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCT CCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCT CAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGA CGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACT CAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACAC TTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTA GCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCT ACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTT AGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTC AGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTG CTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGA AACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTC CGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGC CTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGG AAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGC GATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTT GTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAAC AAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGA TACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTG GAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCT GACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGA GATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGAC ATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGT GTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTA TCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGG CCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTG GAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGC CATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCC TGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTA CTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGT GATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGG AATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATA CTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCC CCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGC TGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGG TAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCAT CGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAA TGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTT TGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTC CCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTA CTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAA TAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGT GGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGG CGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAA TTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTC TGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCA GCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATG GGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTC TTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTC ATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACA TTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGG TTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAA TAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGT AGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGC CTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTT GCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATT CCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCC GCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATA CACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAA AAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATG CAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTC TCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTA TCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAG CATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTT TTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTC CGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAA AGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATAC AGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAAC ATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAG TAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAAT AATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGG GAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGA TAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCC TAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAAT CCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGA AAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTT TTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAG AGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACC TAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATT TAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTA CGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAAT ATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTAT AAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGC GGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATT CTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAG GATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACT CACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCC GACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTT ATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAA GCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCAT CAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCG AGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATG CAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAG GATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGT AACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCAT AAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAAC GCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACA ATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATAC CCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGT TTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGA CAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGA TTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAG GATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGAT GGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTT CAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCG ATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGAT TCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGG TGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCC ATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCT TTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAG TCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAA CAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGAT GATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAA GTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTG CATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGC CAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAA GATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTT CTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGA CTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTT CATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAAT CTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGA GCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAG TTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAA TTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACAT CCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACA TTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAA ACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGA CTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAA AAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTT TTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATAT TTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATAT TTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACC GAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCG CAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTA AGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGA GCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGC GCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACG ATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATC ACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAG CTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAG TTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAAT GCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGA GCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAG TCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTT GTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTG TTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTT ACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTA AGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTT TTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGA CTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTT CGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTT AACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAA ACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTG TACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCAC GTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACC CTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTC ACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAA TAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAA AGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGA GAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACG GCTGACATGGGGCGGCCGCGTAG
[0243] SEQ ID NO: 87 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAA ATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTT CTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTC CTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTG CGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCT GGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGG TGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTT CCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTA CGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTG GCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGC CACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTAC ACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTC CCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAA TTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCT TTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGT CGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGA ACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATC GGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGC ACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGG CAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAAC AGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGC TGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCA ACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATC GCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAA AATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTG CCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAAT AATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTG CAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTAT TAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGAT ATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCT ATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGAT CCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACA TGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGT TCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGA AAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGAT GGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATG CATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCG TGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGC GTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATA CCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAAT GCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACG ACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAA TAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG TAAGGTTCCTAATTCCCCTGGCATCAACTACCTAAATTACTTATCAAACCACTT AGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTG AAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTC AACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCAT GGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTA CAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCA CGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAA TTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAG AAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGT CCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGAT AATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTC TTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTT TATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTG ATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGG AAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGT TTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTT TTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGAT TTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTG AGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGA AGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGC CACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCC GGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGA GCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCAT AAGGTTCCTAAAATGCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCA TATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTT CAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGG ACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGAC CATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATAC AGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTG CTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAG GAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAG GGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGG ATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATG ATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACA ACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCC AGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAAC GGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACA CTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCAC GTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTT GTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTA CAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACAT GCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGC TTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCT TGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTG CCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACC GTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCG AAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAA GCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCA AGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGC TTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATG CTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATAT GTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGA CCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTC CAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATC CAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAA GTTGTAGGGTTCCTAAAAACCCTGGCATCTAAACTAAAATACAATAACCAAAAA TCATTACAAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTA TTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGG AAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTT CACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCAC CTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAG AGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTT TTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGC ACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACG CTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGT CGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTC CTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGC TCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGT CTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTG GGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGG TAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATG GTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGC CACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTC TTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTG CTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTT ACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAA GAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAG GAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCA GAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCG GTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTT ATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTT AACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAAT ATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAAC CGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGC CAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATT CAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCT GATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCT TTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCAC CTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGA TAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCG GGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCAC TTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGT GTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAAT CTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGG CGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTT TCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGT ATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGT GTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGC TGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGT TTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGC ATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTA GAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTC TAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTG GAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGAC GTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACA TGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGG CTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAG AACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAA CTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCC GGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATT ATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACAT TCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGC AGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGG GCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTAC TCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATG ACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCA GCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCA GAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAA GGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACG CCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTAT ATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAG CAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAA AAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAG GTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCA ACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTA TTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGC ATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTG GACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTT GCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTT CTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATA ATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACA TAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAAT AGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTG TAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCT TAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCC TAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAA TATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTG GAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTG TTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCC ATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAA AAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTA ACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACA TGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTT AATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAA TGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAAT TAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAG GAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTC TGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAA AATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGA ATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTAC GCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGC GCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAG GAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCA CCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGC AGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCG GAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACA TCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGG CTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAG CCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGG AGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTA TGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTA ACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGC TGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATC CGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGT GTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTG ATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATA CCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTAC CCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGC TGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAG ACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCT GCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGAT GGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCC GGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTA CAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAA GTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGA ACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGA CGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGT ACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGT TGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTG TTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACAT TACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGA TGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTC TTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGT TAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGG AAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAAT ATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAA CCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCA GTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGC TTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTT CGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTC ACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACC GCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCAT ACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTT TACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAAC GGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTT GGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTA CATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCT TCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAG TTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCC GGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTT CGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCT TCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTAC AATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTC AAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCG TTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTT TTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTC CCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAG GCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTT TAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCC TCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTT GTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACT TTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCC AATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAG GATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCT CATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAAC GAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGC TCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATC AGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATAT AAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATG TAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGG AACACTTAACGGCTGACATGGGGCGGCCGCGTAG
[0244] SEQ ID NO: 88 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATATAAAAA AATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATC TGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAA TTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCC TGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGA GTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGC GAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGG CTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGAT CAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACG TTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCC GCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTG CTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGC AACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAA TTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCA GTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGT GGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATC CTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTA AAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCA TGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGT CACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTG TGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAG TGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTG GTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTA TTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATG AAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGG CATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAA AATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATAT GACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCG CAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTT GGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAA GAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAAT ATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGA AAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATAT ATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCC TACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCC TGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGT GTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCA GAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTG GAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGG GAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGC CGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCAC TGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGC GTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCA GGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCG CCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAA TATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCA GCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGC ATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAACGACCTGG CATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAA AATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAA TTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACT ATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAG TTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTA ATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTAT GTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGAC ATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTT CCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAA AAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTC CCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTG GTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAG TAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATT AATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGG TTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTG CCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGA TTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTC GGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAA CCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCT GGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACA GGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATA CAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGC TTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCA CGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTT CCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGG GTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGAC GTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGA GACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTG GGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTT GTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACT TCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAAT TGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGG AGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGT TCCTAAGGGTCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCCCTAAC CCTTAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCT TTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTG TTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCA GCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCG CCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGG CTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAA TGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGG CGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTT CTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTT CGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAA AACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAA CCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCG CTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGG CGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACC GGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACC ATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGT TTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGC AAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTA ACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAA GTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGAC GCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAAC TGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAA TTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGG TTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTG AAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTAT GGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTT GAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCC GTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAG GTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCG TGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCA ATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAA CCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCC GTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAA TTCCCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATACTACATGTATA AAATTAAAAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCA AAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACT CACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCG ACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTC GAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCC GTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAG ATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCA GCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTC GGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACG GAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCAC TACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCC GTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATT GTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTT GGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAAC ATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGA CCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATAT CCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATT GTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATG ACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCAC ACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAA TGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGA GGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAG GTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGAT GGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTG AGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTT TCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAA CTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAA GCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCAT CACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCG CACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAATGCCTGGCATCAACTA CCTCAATTAATAATCAAACCATCTGAACAATGAATAAATTTAAAAAAATGACAAG TGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGT CGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCC ACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTAC GGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGG AATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCC TTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAA TCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAAT TCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATG CGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAA AGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTT AAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAG TGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGA GAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCT TATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCC ACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTA TTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACA ATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGA TTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGA GAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCA CATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGT GGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTC TTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTT TGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAG AACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGT CCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCAC CCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTT GCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGT TTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGA AGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTAC CAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCT AAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAAACCCTGGCATCA CCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAA TAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACT TGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGG ACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGT TCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTG GTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGT GCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTG CTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGT ATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTT CCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACT GATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCG GTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTG GCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCT GGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTC CGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCA GCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCT GTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCC GTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTT TCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATC GCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCG ATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCA AAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCA ACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCAT TTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATG GTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGA AGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGT GTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTC AGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGC TCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAAC CCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGAT AAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGA AGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCC GGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGT AATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCAT CGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTA CGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATC CCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGC TGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTT AGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACC CTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCC TTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAAT TAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGA AAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCAC ATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTG TGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCT GCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGT GCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGG AATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTG GCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGT GTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGA AACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTT GTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGG AGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACT TTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGC GCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATC CGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCA GGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAAC ACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTG GATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTG CGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGT TATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTG TTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCAT TTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTT TAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATA TTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTT TTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCA GAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATA TACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAA AACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACA AAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAA AATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGC AGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGG CGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATT ATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCA CAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATT CGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATAT GGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATG GCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCA AAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATG GCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAA GAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCG AGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATA GTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTT TACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAAC CAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCAT ATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGA AAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCG ATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATA AGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATG GCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGC TCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCC TGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAAT CGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCT GAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGT GGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAA GAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCAT TGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTC CCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCA TTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCA AGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTA AGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACAT CAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGA GTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGT CAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTA TCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATA CGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACC ATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCC AGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTG ATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGAC GGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGC TTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGG AAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGG ACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACA GAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGT GTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAAC ACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACG GGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTAC CGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTG TCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTT TTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTA CGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATG CGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTT TCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTA AACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAA AGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATAT AAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACC ATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGT CTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTT CGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTC GAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCA CCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCG CTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATA CCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTT ACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACG GGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTG GCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTAC ATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTT CGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGT TTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCG GGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTC GGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTT CGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACA ATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCA AATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGT TGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTT TTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTC CCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAG GCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTT TAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCC TCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTT GTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACT TTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCC AATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAG GATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCT CATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAAC GAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGC TCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATC AGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATAT AAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATG TAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGG AACACTTAACGGCTGACATGGGGCGGCCGCGTAG
[0245] SEQ ID NO: 89 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATATAAAAA AATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATC TGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAA TTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCC TGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGA GTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGC GAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGG CTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGAT CAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACG TTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCC GCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTG CTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGC AACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAA TTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCA GTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGT GGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATC CTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTA AAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCA TGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGT CACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTG TGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAG TGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTG GTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTA TTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATG AAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGG CATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATATAATAA AATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAA TTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACT ATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAG TTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTA ATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTAT GTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGAC ATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTT CCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAA AAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTC CCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTG GTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAG TAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATT AATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGG TTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTG CCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGA TTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTC GGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAA CCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCT GGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACA GGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATA CAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGC TTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCA CGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTT CCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGG GTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGAC GTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGA GACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTG GGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTT GTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACT TCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAAT TGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGG AGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGT TCCTAAACGACCTGGCATCATCTACCTATATTACTAAACAAACCAATTATAATA ATTTTAAAAATAAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCT TATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTC TTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGAT GATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTT TATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAA AAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCG CCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCA ACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTA AAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGAT GGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCG GATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGG CCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCC ACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTC ACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCA ATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTG CGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGC AGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGG ACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGG TGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAA CTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAA TCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGC AACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTT CCTAAGGGTCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCG TAAAAAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGT CGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATC TCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTC TCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACAC CGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGG CGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGA TTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAG AAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCT GGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGC GACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTG GCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTC ATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGG TGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCT CGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCA ACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGT GCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACG GAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGT GATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTAT GCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAG GTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGAT CCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTA TAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAG GCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATAC CGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGA GGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGA CGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGA TTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATT AAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTT CATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCG TCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAATTCCCCTGGCATCCC CAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAATTAAAAAATGAGT AACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCT GAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGA ACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCAC GAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACC TGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCC CTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAG TGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGA TGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAA GACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTC CAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCT GTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACA GATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCC AAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACG ACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGG TATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACC CGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTC TGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAA GAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTA TCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAA GCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAG CCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAAC CCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCA TGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGAC AACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGG TACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCT GTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTC CGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCA TCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGC GTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGC ATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTC TGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGA TCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGT AAAGTTGGCAAGAAATAAGGTTCCTAAAATGCCTGGCATCAACTACCTCAATTA ATAATCAAACCATCTGAACAATGAATAAATTTAAAAAAATGACAAGTGCCGCTA CTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTA TTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTG GGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTT TTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGT CGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCA ATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGA TTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTG CCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTT AGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTA CCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTG GGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACC TCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTT GAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGA TGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAA CTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGAC CAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATG ATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGA TCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATT CAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGA CCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCC ACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGG GCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGC TAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCA GAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGG CAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGC TCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGT GTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAA GGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAG CCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCG CTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGC AGAAATAAGAGTAAGTTGTAGGGTTCCTAAAAACCCTGGCATCATCTACCTATA TTATTCATCAAACCATTTGATCCAACATTAAATATTAAAAAATGAATAATCCACA AACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAG CAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTAC GCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGG CTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTAC TTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGT TCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATG CTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATG AACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCA CCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTC CGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCA TGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAA GGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAAT GCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACT TTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGG TCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATG GTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAA ACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGAC GATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCT TCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTC GGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAAC TGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGA TGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTG GCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATT GTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCAT CCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAAT AATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATC GGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCC ACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCC GCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATG GAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATG ACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTT ACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCAC TGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGT CTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGA ATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGT TGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAA AACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCA TTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGA ATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTT GCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATT TAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTG ATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGAT GAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTT GGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTT GTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCG CGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCAT CTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCAT TTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGAT TTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCG GATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCC ACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAA ATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTT CTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGA TAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCC CTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCC GTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCT CGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACAC CAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAA GGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAG CGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCT CTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCG TAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCAT TTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTG CATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGA CCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGA TGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGA CCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAA ATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAA TATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATG AATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAA GGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGA AATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAAC TATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCAT ATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAA TTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGC TAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGT AGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAA GTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAA GAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAA AAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATG ATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAAT ATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGT ACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGAT TAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTA TCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCG AGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTC GTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAA GTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTT ATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAA ATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGC GAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAAC CGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATA TTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACC ATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAAT TCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCT ACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATC GATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCA TATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTC CCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAG TTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTT TGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGA TCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGG CCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCA GAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGAT ACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTC AAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTG TGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATT TCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTG TATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCG GTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAG TGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGAT GCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTA TGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCAT ATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAG TCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGAT ATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTA CAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTT TCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCAT TCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTG CCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTT CGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGT TCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTAT TTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCG GTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTAT TTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAA AACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGA GCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAA GGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAG GAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCA GGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTG CTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAG GATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGA TATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGAC GGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAG CAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTC TACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATA CACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACG AGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGA TAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGT GCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAA TCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGT GGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTC ACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTG CGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGC AGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAG GTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCT TCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGC GGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCAT CAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACA CTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAA TAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAA AGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGT ACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACT AAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCAT GGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGT ACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGC CTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCT TTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAG CCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTG ACATGGGGCGGCCGCGTAG
[0246] SEQ ID NO: 90 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAA AATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATC TGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAA TTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCC TGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGA GTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGC GAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGG CTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGAT CAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACG TTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCC GCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTG CTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGC AACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAA TTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCA GTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGT GGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATC CTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTA AAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCA TGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGT CACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTG TGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAG TGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTG GTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTA TTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATG AAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGG CATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAA AATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCG ATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCT ATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTT CCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAA GGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCG TGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGC GGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAAT GGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACA GCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCC GGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGA CCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCT CCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCA AGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACA ATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTG CCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTG CTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGG CGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGAC GAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAA CCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTG CTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCG CTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGAC TGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTC GTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAAT GCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCT TGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCT GCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCAT TCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTT ACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCC ACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGA TGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTG AAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACG CGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAA CGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAACGACCTGGCATCAAC TACCTACATTAATAACCAACCCAAATGCCTAATCAATAAATATTATAAAATGAAT AATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTT GGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGA CACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTT CAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGG TGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTG CGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCT CTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTAT CGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCC GGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGA TCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGT TTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGC CAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGG CGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCG TTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGC CTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGT TCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGT TACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTC CCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCG CATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGAT ATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAA AGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAAC TTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTT GCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGT CAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTCCCCTGGC ATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAA ATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATAT GACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCG CAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTT GGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAA GAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAAT ATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGA AAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATAT ATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCC TACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCC TGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGT GTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCA GAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTG GAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGG GAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGC CGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCAC TGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGC GTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCA GGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCG CCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAA TATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCA GCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGC ATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAAACCCTGG CATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAA AATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATC CGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGG ATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTC CATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAAT GGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGG CGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGA CCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTT CTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCT TCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAAC TACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAAT CCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCA TACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACA TTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGAC AGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTA CTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCA AAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTT CCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACA CATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAA GCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAA GCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGG TACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGAC AATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACG TCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAG AGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTT TTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGA TTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAA GCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCG CCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTC TTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGAT AAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAAC TTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTA GAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAG GTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTAT TCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCG GCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAA ACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGG ATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCT GAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTAT CCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTAT GTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGC ATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCT ACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACAT CCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGG CTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATT TAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTAC CCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTC CTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAA TTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGG AAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCA CATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTT GTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGC TGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCG TGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTG GAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATT GGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGG TGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTG AAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTT TGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTG GAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTA CTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTG GCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCA TCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAG CAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGA ACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGT TGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCG TGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGG GTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGG TGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATC ATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAA TTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTC ATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATAC CTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACT CAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTG ATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCA AAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGA ACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGA AAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGG TGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAA CGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAA ATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAG CCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATAT AATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAA TATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTA ATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCAT CCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAA ATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAAC AAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATG CGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAA TAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATG TTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTA ACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTC ATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGA GAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTG CGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAA TAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAAT GGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACG CTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGC CTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGA ATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACC TGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAG TGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGA AGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATC ATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCT TCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCC CATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAG CAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAAC ATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTG AGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCC GTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTG TATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGA TACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATAC CATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACC CAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCT GATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGA CGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTG CTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATG GAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCG GACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTAC AGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAG TGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAA CACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGAC GGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTA CCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTT GTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGT TTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATT ACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGAT GCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCT TTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTT AAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGA AAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATA TAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAAC CATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAG TCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCT TCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTC GAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCA CCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCG CTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATA CCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTT ACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACG GGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTG GCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTAC ATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTT CGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGT TTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCG GGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTC GGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTT CGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACA ATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCA AATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGT TGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTT TTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTC CCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAG GCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTT TAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCC TCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTT GTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACT TTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCC AATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAG GATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCT CATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAAC GAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGC TCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATC AGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATAT AAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATG TAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGG AACACTTAACGGCTGACATGGGGCGGCCGCGTAG
[0247] SEQ ID NO: 91 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAA ATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTT CTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTC CTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTG CGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCT GGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGG TGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTT CCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTA CGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTG GCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGC CACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTAC ACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTC CCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAA TTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCT TTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGT CGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGA ACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATC GGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGC ACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGG CAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAAC AGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGC TGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCA ACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATC GCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAA AATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTG CCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAAT AATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTG CAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTAT TAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGAT ATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCT ATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGAT CCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACA TGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGT TCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGA AAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGAT GGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATG CATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCG TGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGC GTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATA CCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAAT GCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACG ACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAA TAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG TAAGGTTCCTAATTCCCCTGGCATCAACTACCTAAATTACTTATCAAACCACTT AGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTG AAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTC AACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCAT GGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTA CAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCA CGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAA TTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAG AAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGT CCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGAT AATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTC TTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTT TATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTG ATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGG AAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGT TTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTT TTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGAT TTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTG AGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGA AGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGC CACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCC GGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGA GCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCAT AAGGTTCCTAAAAACCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCA TATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTT CAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGG ACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGAC CATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATAC AGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTG CTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAG GAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAG GGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGG ATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATG ATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACA ACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCC AGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAAC GGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACA CTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCAC GTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTT GTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTA CAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACAT GCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGC TTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCT TGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTG CCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACC GTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCG AAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAA GCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCA AGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGC TTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATG CTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATAT GTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGA CCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTC CAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATC CAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAA GTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTT TGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTAT CTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGA AAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTG CACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAA GGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAG AAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGG TGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGAC CTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTC CTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGA GTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAA TCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGA TGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGA AAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAG GAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAA TTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTT TAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGT TAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCC CTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTC GGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGT TGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCT TTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAG TGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTG GGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAA ATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTAC AGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGC TTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGG AGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAA GACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGT CGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCT GGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCG GTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGC GAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCAC CTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAAC CCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAA TCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAA GTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTT GCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAA CAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATA TAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTT AAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAA GAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGT AGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGAT GAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCA ATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAAT TTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATG AAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGA TTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGT GAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGAT GATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGC ATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAG CCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATAC AAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGC AAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAAT TGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGA AACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAA TCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGT TTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAAC AATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGG AGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAG CATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGA AAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGAT GGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAAC CTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAG TGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACT TGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAA ACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTG CCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGA ATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGT ACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTA GTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCA GAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCT GATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCAT GTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCA TAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATG ATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGC TTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCC GAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGC CGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGT CTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCAT AACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTA CCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACA TCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAG CAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACAT TTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTT ATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGA TAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTG TGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTA CGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATG ATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCG GACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTC TTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAAC GCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCA GCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGA CTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAA TGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCA TTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTAT CATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTT TCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGC TACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTG AAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTA GCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATC CTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGG ATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTT TTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCT CGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTG ATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCG GTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATC CATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATC TTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAA GTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGA AACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACA GATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAA GAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTC CTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACG TGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGC AAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGA GTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCC TGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCA GTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGA AATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGA GAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGA AGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAA AATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCAC ATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAA CATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGC AAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTC GACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAAC CTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTT AACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAA GCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGT TTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAG TTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGG TTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCT CAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCC GCGTAG
[0248] SEQ ID NO: 92 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAA AATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAA TTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACT ATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAG TTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTA ATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTAT GTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGAC ATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTT CCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAA AAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTC CCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTG GTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAG TAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATT AATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGG TTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTG CCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGA TTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTC GGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAA CCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCT GGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACA GGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATA CAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGC TTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCA CGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTT CCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGG GTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGAC GTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGA GACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTG GGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTT GTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACT TCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAAT TGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGG AGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGT TCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGA AAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTT CACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGC GCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCA GCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAA CAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGG AAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCC ATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCT GATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAA AGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGG TTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAAC ACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAA AACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAAC CTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCT GGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGC GGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAG GCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACT GGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGC ATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAAT CGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTC AGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATC CCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGG AAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATAT TAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTG GGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAG GTTCCTAAACGACCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTA ATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGC TTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTT CTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTG ATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGT TTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGAT AAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTC GCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATC AACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCT AAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGA TGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGC GGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATG GCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGC CACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGT CACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTC AATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCT GCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAG CAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCG GACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTG GTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAA ACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTA ATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGG CAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGG TTCCTAAGGGTCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACCTTTTA ACATAAAAATTAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCT TGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCT CGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTT GGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCA AGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAA TTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGT ATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGG TGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGG ACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGT TCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCG CAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTT TCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACC GACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCT GTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACA GCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCG GTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTC GTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAAT GCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTT TATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGT TCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTAT GGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGG GTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCG ATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGC CGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTA AGGTTCCTAATTCCCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAAC AATTTAAAAAAAATTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATG GCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATG GTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGG TCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTA TCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCC TGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTC CCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACA GGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAA GCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGC AGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCA GCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTT CAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTC GCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAA AGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAA ACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACA ACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGA CGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAA GGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGG CTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATC AAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTG ACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAA ACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCC AATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCT GGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGC TGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGT ATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTG TTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGC CGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAA GGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCC GTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCC AATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAA ACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGC CGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTA AAATGCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAA AAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCC AAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACT CACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCG ACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTC GAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCC GTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAG ATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCA GCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTC GGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACG GAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCAC TACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCC GTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATT GTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTT GGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAAC ATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGA CCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATAT CCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATT GTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATG ACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCAC ACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAA TGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGA GGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAG GTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGAT GGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTG AGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTT TCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAA CTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAA GCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCAT CACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCG CACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAAACCCTGGCATCTTTTC CATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAG TGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGT CGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCC ACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTAC GGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGG AATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCC TTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAA TCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAAT TCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATG CGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAA AGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTT AAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAG TGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGA GAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCT TATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCC ACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTA TTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACA ATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGA TTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGA GAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCA CATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGT GGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTC TTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTT TGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAG AACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGT CCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCAC CCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTT GCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGT TTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGA AGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTAC CAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCT AAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCC ATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGA ATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCA TCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTG CCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACT CCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGA TGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAA TGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACT TTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCC ACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTG GTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTA GAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCG GTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATG TAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTG GCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCC CGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACT TTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATA ATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTG GTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGC GATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATT CTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTG TTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCT CGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGG CATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTT CATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCAT GATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATT CGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTT CCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAAT AAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTA GTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCC TGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTG CCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTC CCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCG CTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATAC ACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAA AAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGC AGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCT CTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTAT CGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGC ATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTT TGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCC GACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAA GATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACA GACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACA TAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGT AAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATA ATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGG AAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATA GAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTA ACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCC ATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAA AATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTT GCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAG GTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTA AAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTA AAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACG AAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATA TGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAA ATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGG GTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTG ATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGAT TATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCAC CGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGAC TCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATC AAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGC TTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCA AAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAG GCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCA ACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGG ATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTA ACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATA AATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAAC GCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACA ATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATAC CCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGT TTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGA CAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGA TTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAG GATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGAT GGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTT CAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCG ATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGAT TCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGG TGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCC ATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCT TTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAG TCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAA CAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGAT GATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAA GTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTG CATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGC CAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAA GATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTT CTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGA CTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTT CATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAAT CTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGA GCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAG TTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAA TTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACAT CCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACA TTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAA ACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGA CTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAA AAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTT TTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATAT TTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATAT TTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACC GAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCG CAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTA AGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGA GCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGC GCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACG ATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATC ACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAG CTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAG TTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAAT GCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGA GCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAG TCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTT GTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTG TTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTT ACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTA AGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTT TTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGA CTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTT CGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTT AACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAA ACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTG TACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCAC GTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACC CTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTC ACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAA TAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAA AGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGA GAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACG GCTGACATGGGGCGGCCGCGTAG
[0249] SEQ ID NO: 93 (Plasmídeo que codifica a via de degradação de oxalato para integração genômica no genoma de Bacteroides vulgatus):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAA ATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTT CTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTC CTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTG CGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCT GGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGG TGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTT CCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTA CGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTG GCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGC CACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTAC ACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTC CCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAA TTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCT TTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGT CGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGA ACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATC GGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGC ACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGG CAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAAC AGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGC TGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCA ACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATC GCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAA AATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTG CCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAAT AATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTG CAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTAT TAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGAT ATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCT ATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGAT CCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACA TGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGT TCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGA AAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGAT GGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATG CATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCG TGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGC GTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATA CCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAAT GCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACG ACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAA TAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG TAAGGTTCCTAAGGGTCCTGGCATCAACTACCTAAATTACTTATCAAACCACTT AGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTG AAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTC AACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCAT GGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTA CAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCA CGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAA TTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAG AAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGT CCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGAT AATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTC TTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTT TATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTG ATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGG AAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGT TTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTT TTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGAT TTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTG AGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGA AGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGC CACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCC GGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGA GCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCAT AAGGTTCCTAATTCCCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCA TATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTT CAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGG ACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGAC CATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATAC AGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTG CTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAG GAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAG GGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGG ATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATG ATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACA ACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCC AGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAAC GGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACA CTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCAC GTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTT GTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTA CAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACAT GCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGC TTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCT TGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTG CCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACC GTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCG AAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAA GCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCA AGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGC TTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATG CTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATAT GTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGA CCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTC CAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATC CAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAA GTTGTAGGGTTCCTAAAATGCCTGGCATCAACTACCTAAATTAATTATCACCCC AAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTG ACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACC ATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGA TGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAG CTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCC GCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACT GCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTG CAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACT GCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCT CGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACT TGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTG CTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTAT CGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTG GGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTG AAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCC TGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAG TGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACG GTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACAT CCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGT GACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAA AAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAA AGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAAC TACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATAT TTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTG ACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGG TATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTA TCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAAC CATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTA TCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGT CTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTT ATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCT TCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCG AATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAA GGTTCCTAAAAACCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATTAC AAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAA ACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTC AATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCT TGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTA ACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACG GCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCT CCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCT CAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGA CGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACT CAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACAC TTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTA GCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCT ACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTT AGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTC AGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTG CTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGA AACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTC CGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGC CTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGG AAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGC GATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTT GTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAAC AAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGA TACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTG GAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCT GACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGA GATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGAC ATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGT GTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTA TCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGG CCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTG GAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGC CATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCC TGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTA CTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGT GATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGG AATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATA CTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCC CCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGC TGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGG TAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCAT CGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAA TGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTT TGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTC CCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTA CTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAA TAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGT GGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGG CGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAA TTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTC TGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCA GCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATG GGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTC TTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTC ATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACA TTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGG TTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAA TAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGT AGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGC CTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTT GCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATT CCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCC GCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATA CACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAA AAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATG CAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTC TCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTA TCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAG CATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTT TTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTC CGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAA AGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATAC AGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAAC ATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAG TAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAAT AATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGG GAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGA TAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCC TAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAAT CCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGA AAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTT TTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAG AGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACC TAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATT TAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTA CGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAAT ATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTAT AAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGC GGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATT CTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAG GATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACT CACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCC GACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTT ATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAA GCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCAT CAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCG AGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATG CAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAG GATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGT AACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCAT AAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAAC GCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACA ATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATAC CCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGT TTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGA CAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGA TTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAG GATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGAT GGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTT CAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCG ATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGAT TCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGG TGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCC ATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCT TTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAG TCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAA CAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGAT GATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAA GTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTG CATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGC CAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAA GATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTT CTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGA CTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTT CATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAAT CTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGA GCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAG TTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAA TTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACAT CCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACA TTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAA ACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGA CTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAA AAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTT TTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATAT TTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATAT TTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACC GAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCG CAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTA AGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGA GCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGC GCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACG ATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATC ACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAG CTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAG TTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAAT GCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGA GCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAG TCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTT GTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTG TTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTT ACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTA AGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTT TTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGA CTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTT CGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTT AACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAA ACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTG TACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCAC GTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACC CTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTC ACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAA TAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAA AGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGA GAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACG GCTGACATGGGGCGGCCGCGTAG
[0250] SEQ ID NO: 94 (Sítio de ligação ao ribossomo e quadro de leitura aberto At_AAE3):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAA ATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTT CTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTC CTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTG CGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCT GGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGG TGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTT CCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTA CGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTG GCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGC CACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTAC ACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTC CCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAA TTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCT TTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGT CGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGA ACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATC GGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGC ACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGG CAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAAC AGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGC TGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCA ACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATC GCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAA AATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTG CCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAAT AATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTG CAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTAT TAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGAT ATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCT ATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGAT CCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACA TGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGT TCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGA AAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGAT GGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATG CATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCG TGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGC GTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATA CCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAAT GCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACG ACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAA TAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG TAAGGTTCCTAATTCCCCTGGCATCAACTACCTAAATTACTTATCAAACCACTT AGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTG AAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTC AACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCAT GGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTA CAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCA CGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAA TTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAG AAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGT CCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGAT AATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTC TTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTT TATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTG ATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGG AAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGT TTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTT TTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGAT TTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTG AGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGA AGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGC CACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCC GGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGA GCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCAT AAGGTTCCTAAAATGCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCA TATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTT CAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGG ACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGAC CATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATAC AGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTG CTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAG GAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAG GGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGG ATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATG ATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACA ACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCC AGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAAC GGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACA CTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCAC GTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTT GTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTA CAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACAT GCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGC TTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCT TGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTG CCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACC GTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCG AAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAA GCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCA AGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGC TTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATG CTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATAT GTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGA CCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTC CAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATC CAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAA GTTGTAGGGTTCCTAAAAACCCTGGCATCTAAACTAAAATACAATAACCAAAAA TCATTACAAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTA TTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGG AAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTT CACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCAC CTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAG AGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTT TTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGC ACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACG CTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGT CGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTC CTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGC TCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGT CTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTG GGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGG TAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATG GTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGC CACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTC TTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTG CTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTT ACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAA GAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAG GAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCA GAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCG GTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTT ATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTT AACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAAT ATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAAC CGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGC CAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATT CAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCT GATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCT TTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCAC CTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGA TAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCG GGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCAC TTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGT GTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAAT CTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGG CGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTT TCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGT ATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGT GTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGC TGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGT TTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGC ATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTA GAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTC TAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTG GAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGAC GTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACA TGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGG CTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAG AACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAA CTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCC GGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATT ATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACAT TCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGC AGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGG GCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTAC TCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATG ACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCA GCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCA GAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAA GGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACG CCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTAT ATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAG CAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAA AAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAG GTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCA ACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTA TTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGC ATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTG GACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTT GCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTT CTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATA ATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACA TAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAAT AGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTG TAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCT TAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCC TAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAA TATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTG GAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTG TTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCC ATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAA AAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTA ACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACA TGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTT AATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAA TGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAAT TAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAG GAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTC TGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAA AATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGA ATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTAC GCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGC GCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAG GAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCA CCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGC AGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCG GAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACA TCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGG CTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAG CCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGG AGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTA TGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTA ACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGC TGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATC CGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGT GTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTG ATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATA CCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTAC CCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGC TGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAG ACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCT GCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGAT GGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCC GGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTA CAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAA GTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGA ACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGA CGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGT ACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGT TGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTG TTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACAT TACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGA TGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTC TTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGT TAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGG AAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAAT ATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAA CCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCA GTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGC TTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTT CGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTC ACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACC GCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCAT ACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTT TACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAAC GGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTT GGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTA CATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCT TCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAG TTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCC GGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTT CGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCT TCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTAC AATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTC AAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCG TTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTT TTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTC CCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAG GCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTT TAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCC TCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTT GTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACT TTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCC AATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAG GATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCT CATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAAC GAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGC TCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATC AGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATAT AAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATG TAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGG AACACTTAACGGCTGACATGGGGCGGCCGCGTAG
[0251] SEQ ID NO: 95 (Sítio de ligação ao ribossomo e quadro de leitura aberto At_AAE3):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAG CAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAA CGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCG GAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAG TCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGC CGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAA GATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCC CTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATAT GATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCA TGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGAC TTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGA TATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCC GTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAAC GGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGT GACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAG TTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGA TCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAA ACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAA AGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCA AGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGA TTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCG TGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAA AGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAG TGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGT GGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGA TGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCA TTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACC GGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACT GCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTT TTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGA ACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGG CATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATATAAAAA AATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATC TGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAA TTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCC TGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGA GTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGC GAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGG CTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGAT CAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACG TTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCC GCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTG CTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGC AACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAA TTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCA GTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGT GGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATC CTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTA AAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCA TGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGT CACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTG TGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAG TGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTG GTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTA TTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATG AAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGG CATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAA AATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATAT GACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCG CAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTT GGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAA GAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAAT ATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGA AAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATAT ATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCC TACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCC TGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGT GTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCA GAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTG GAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGG GAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGC CGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCAC TGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGC GTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCA GGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCG CCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAA TATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCA GCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGC ATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAACGACCTGG CATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAA AATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAA TTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACT ATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAG TTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTA ATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTAT GTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGAC ATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTT CCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAA AAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTC CCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTG GTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAG TAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATT AATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGG TTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTG CCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGA TTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTC GGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAA CCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCT GGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACA GGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATA CAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGC TTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCA CGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTT CCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGG GTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGAC GTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGA GACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTG GGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTT GTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACT TCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAAT TGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGG AGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGT TCCTAAGGGTCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCCCTAAC CCTTAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCT TTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTG TTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCA GCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCG CCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGG CTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAA TGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGG CGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTT CTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTT CGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAA AACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAA CCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCG CTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGG CGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACC GGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACC ATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGT TTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGC AAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTA ACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAA GTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGAC GCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAAC TGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAA TTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGG TTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTG AAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTAT GGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTT GAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCC GTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAG GTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCG TGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCA ATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAA CCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCC GTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAA TTCCCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATACTACATGTATA AAATTAAAAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCA AAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACT CACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCG ACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTC GAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCC GTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAG ATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCA GCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTC GGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACG GAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCAC TACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCC GTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATT GTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTT GGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAAC ATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGA CCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATAT CCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATT GTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATG ACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCAC ACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAA TGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGA GGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAG GTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGAT GGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTG AGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTT TCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAA CTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAA GCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCAT CACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCG CACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAATGCCTGGCATCAACTA CCTCAATTAATAATCAAACCATCTGAACAATGAATAAATTTAAAAAAATGACAAG TGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGT CGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCC ACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTAC GGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGG AATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCC TTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAA TCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAAT TCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATG CGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAA AGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTT AAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAG TGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGA GAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCT TATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCC ACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTA TTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACA ATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGA TTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGA GAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCA CATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGT GGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTC TTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTT TGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAG AACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGT CCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCAC CCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTT GCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGT TTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGA AGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTAC CAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCT AAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAAACCCTGGCATCA CCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAA TAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACT TGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGG ACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGT TCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTG GTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGT GCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTG CTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGT ATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTT CCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACT GATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCG GTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTG GCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCT GGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTC CGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCA GCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCT GTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCC GTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTT TCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATC GCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCG ATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCA AAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCA ACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCAT TTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATG GTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGA AGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGT GTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTC AGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGC TCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAAC CCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGAT AAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGA AGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCC GGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGT AATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCAT CGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTA CGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATC CCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGC TGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTT AGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACC CTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCC TTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAAT TAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGA AAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCAC ATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTG TGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCT GCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGT GCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGG AATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTG GCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGT GTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGA AACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTT GTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGG AGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACT TTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGC GCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATC CGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCA GGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAAC ACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTG GATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTG CGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGT TATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTG TTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCAT TTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTT TAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATA TTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTT TTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCA GAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATA TACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAA AACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACA AAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAA AATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGC AGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGG CGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATT ATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCA CAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATT CGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATAT GGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATG GCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCA AAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATG GCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAA GAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCG AGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATA GTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTT TACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAAC CAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCAT ATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGA AAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCG ATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATA AGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATG GCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGC TCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCC TGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAAT CGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCT GAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGT GGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAA GAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCAT TGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTC CCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCA TTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCA AGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTA AGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACAT CAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGA GTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGT CAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTA TCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATA CGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACC ATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCC AGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTG ATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGAC GGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGC TTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGG AAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGG ACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACA GAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGT GTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAAC ACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACG GGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTAC CGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTG TCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTT TTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTA CGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATG CGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTT TCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTA AACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAA AGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATAT AAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACC ATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGT CTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTT CGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTC GAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCA CCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCG CTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATA CCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTT ACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACG GGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTG GCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTAC ATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTT CGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGT TTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCG GGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTC GGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTT CGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACA ATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCA AATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGT TGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTT TTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTC CCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAG GCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTT TAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCC TCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTT GTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACT TTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCC AATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAG GATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCT CATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAAC GAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGC TCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATC AGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATAT AAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATG TAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGG AACACTTAACGGCTGACATGGGGCGGCCGCGTAG
[0252] SEQ ID NO: 96 (Sítio de ligação ao ribossomo RBS1 e quadro de leitura aberto At_AAE3):
GCATCTAAACTAAAATACAATAACCAAAAATCATTACAAATGAAAAAAA ATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAA AATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCAC GCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACG CTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGA GTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGT TGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGAT TCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGC TTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGG ACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAA CTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTAC GAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTC AAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTT CTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGG AGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACAT TTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACC ATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCC TAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGT CCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGAC AGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACAC AAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATG AGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGG TCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTT TCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGG TATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAA GATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCA GGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTG CGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTT TGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGA TAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAAC ATTTCCTTGAGAAGCCC
[0253] SEQ ID NO: 97 (Sítio de ligação ao ribossomo RBS2 e quadro de leitura aberto At_AAE3):
GCATCTAAACTAAAATACAATAACCAAAAATCATGTTTAACAAATAAAA ATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAA AATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCAC GCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACG CTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGA GTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGT TGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGAT TCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGC TTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGG ACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAA CTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTAC GAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTC AAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTT CTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGG AGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACAT TTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACC ATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCC TAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGT CCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGAC AGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACAC AAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATG AGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGG TCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTT TCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGG TATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAA GATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCA GGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTG CGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTT TGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGA TAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAAC ATTTCCTTGAGAAGCCC
[0254] SEQ ID NO: 98 (Sítio de ligação ao ribossomo RBS3 e quadro de leitura aberto At_AAE3):
GCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAAAAAA ATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAA AATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCAC GCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACG CTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGA GTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGT TGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGAT TCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGC TTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGG ACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAA CTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTAC GAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTC AAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTT CTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGG AGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACAT TTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACC ATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCC TAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGT CCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGAC AGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACAC AAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATG AGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGG TCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTT TCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGG TATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAA GATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCA GGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTG CGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTT TGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGA TAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAAC ATTTCCTTGAGAAGCCC
[0255] SEQ ID NO: 99 (Sítio de ligação ao ribossomo RBS4 e quadro de leitura aberto At_AAE3):
GCATCTAAACTAAAATACAATAACCAAAAATCATACTACATGTATAAAA TTAAAAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAA AATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCAC GCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACG CTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGA GTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGT TGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGAT TCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGC TTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGG ACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAA CTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTAC GAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTC AAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTT CTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGG AGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACAT TTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACC ATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCC TAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGT CCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGAC AGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACAC AAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATG AGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGG TCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTT TCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGG TATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAA GATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCA GGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTG CGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTT TGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGA TAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAAC ATTTCCTTGAGAAGCCC
[0256] SEQ ID NO: 100 (Sítio de ligação ao ribossomo RBS5 e quadro de leitura aberto At_AAE3):
GCATCTAAACTAAAATACAATAACCAAAAATCATTTTCACAATATAAAAA TAACAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAA AATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCAC GCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACG CTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGA GTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGT TGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGAT TCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGC TTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGG ACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAA CTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTAC GAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTC AAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTT CTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGG AGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACAT TTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACC ATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCC TAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGT CCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGAC AGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACAC AAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATG AGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGG TCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTT TCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGG TATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAA GATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCA GGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTG CGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTT TGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGA TAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAAC ATTTCCTTGAGAAGCCC
[0257] SEQ ID NO: 101 (Sítio de ligação ao ribossomo RBS6 e quadro de leitura aberto At_AAE3):
GCATCATCTACCTCAATTATTCACCATTCCATCTGTCTAGCCAAAAAAT TTAAAAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAA AATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCAC GCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACG CTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGA GTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGT TGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGAT TCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGC TTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGG ACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAA CTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTAC GAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTC AAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTT CTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGG AGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACAT TTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACC ATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCC TAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGT CCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGAC AGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACAC AAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATG AGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGG TCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTT TCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGG TATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAA GATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCA GGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTG CGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTT TGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGA TAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAAC ATTTCCTTGAGAAGCCC
[0258] SEQ ID NO: 102 (Sítio de ligação ao ribossomo RBS7 e quadro de leitura aberto At_AAE3):
GCATCATCTACCTAAATTACTCATCATCCCATTTTAGTCAGCTTAAAAT ATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAA AATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCAC GCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACG CTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGA GTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGT TGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGAT TCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGC TTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGG ACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAA CTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTAC GAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTC AAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTT CTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGG AGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACAT TTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACC ATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCC TAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGT CCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGAC AGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACAC AAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATG AGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGG TCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTT TCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGG TATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAA GATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCA GGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTG CGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTT TGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGA TAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAAC ATTTCCTTGAGAAGCCC
[0259] SEQ ID NO: 103 (Sítio de ligação ao ribossomo RBS8 e quadro de leitura aberto At_AAE3):
GCATCATCTACCTATATTATTTATCATTCCATATCATGCGTGAATAAAAA TAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAA ATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACG CGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGC TGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGT TTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTG AACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTC AAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTT CAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGA CCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAAC TCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACG AGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAA GAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCT CCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAG CTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTT TGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCAT TCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTA AACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCC AGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAG AGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAA GCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAG AAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTC CAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTC GAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTA TTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAG ATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAG GGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGC GGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTT GTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGAT AACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACA TTTCCTTGAGAAGCCC
[0260] SEQ ID NO: 104 (Sítio de ligação ao ribossomo RBS1 e quadro de leitura aberto Sc_YBR222C):
GCATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAAT TAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAG CGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCT ACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCT AATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCAT GCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTA AAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATT TGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTA CAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAG CTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGA GGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCA ACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTG ATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACAT TTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCC CTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTG GTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGAT TCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCA GTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCT TTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACG TTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAA AGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGA CAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGC GAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTT CACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTG AGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
[0261] SEQ ID NO: 105 (Sítio de ligação ao ribossomo RBS2 e quadro de leitura aberto Sc_YBR222C):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAA ATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTA GCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACC TACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCC TAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCAT GCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTA AAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATT TGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTA CAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAG CTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGA GGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCA ACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTG ATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACAT TTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCC CTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTG GTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGAT TCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCA GTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCT TTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACG TTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAA AGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGA CAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGC GAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTT CACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTG AGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
[0262] SEQ ID NO: 106 (Sítio de ligação ao ribossomo RBS3 e quadro de leitura aberto Sc_YBR222C):
GCATCAACTACCTCAATTAATAATCAAACCATCTGAACAATGAATAAAT TTAAAAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTA GCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACC TACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCC TAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCAT GCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTA AAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATT TGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTA CAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAG CTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGA GGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCA ACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTG ATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACAT TTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCC CTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTG GTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGAT TCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCA GTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCT TTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACG TTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAA AGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGA CAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGC GAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTT CACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTG AGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
[0263] SEQ ID NO: 107 (Sítio de ligação ao ribossomo RBS4 e quadro de leitura aberto Sc_YBR222C):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAGATATAAAA TTAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTA GCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACC TACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCC TAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCAT GCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTA AAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATT TGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTA CAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAG CTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGA GGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCA ACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTG ATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACAT TTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCC CTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTG GTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGAT TCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCA GTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCT TTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACG TTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAA AGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGA CAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGC GAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTT CACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTG AGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
[0264] SEQ ID NO: 108 (Sítio de ligação ao ribossomo RBS5 e quadro de leitura aberto Sc_YBR222C):
GCATCAACTACCTTAATTATTCATCAAACCATATAACATACTAAAAAAAA TAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAG CGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCT ACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCT AATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCAT GCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTA AAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATT TGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTA CAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAG CTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGA GGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCA ACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTG ATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACAT TTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCC CTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTG GTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGAT TCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCA GTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCT TTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACG TTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAA AGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGA CAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGC GAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTT CACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTG AGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
[0265] SEQ ID NO: 109 (Sítio de ligação ao ribossomo RBS6 e quadro de leitura aberto Sc_YBR222C):
GCATCATCTACCTATATTACTAATCATTCCAATTAATTACCTTATAAAAT TAAGAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAG CGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCT ACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCT AATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCAT GCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTA AAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATT TGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTA CAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAG CTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGA GGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCA ACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTG ATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACAT TTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCC CTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTG GTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGAT TCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCA GTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCT TTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACG TTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAA AGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGA CAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGC GAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTT CACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTG AGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
[0266] SEQ ID NO: 110 (Sítio de ligação ao ribossomo RBS7 e quadro de leitura aberto Sc_YBR222C):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCCCCCCACGTTTAAAA TTAAAAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTA GCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACC TACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCC TAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCAT GCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTA AAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATT TGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTA CAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAG CTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGA GGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCA ACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTG ATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACAT TTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCC CTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTG GTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGAT TCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCA GTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCT TTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACG TTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAA AGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGA CAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGC GAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTT CACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTG AGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
[0267] SEQ ID NO: 111 (Sítio de ligação ao ribossomo RBS8 e quadro de leitura aberto Sc_YBR222C):
GCATCAACTACCTTCATTACTTAACAATCCACCTGTTGTGAGTAAAAAT ATAAGAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTA GCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACC TACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCC TAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCAT GCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTA AAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATT TGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTA CAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAG CTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGA GGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCA ACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTG ATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACAT TTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCC CTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTG GTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGAT TCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCA GTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCT TTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACG TTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGAT GACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAA AGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGA CAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGC GAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTT CACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTG AGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGT GAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGAT CGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTC AAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTG AACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTT GTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGC AGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
[0268] SEQ ID NO: 112 (Sítio de ligação ao ribossomo RBS1 e quadro de leitura aberto Of_oxc):
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCCCTAACCCTTAAAATA TAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTT GATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCA TTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTAC AGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACAT CGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAAC GGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTT GAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAA GAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTAT CAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTG TATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGT CAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCC AGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTG ATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACG CACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATT CCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCC GCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGA TCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGG CGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACA GCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTC CCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACC GGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGA TGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTT GTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGC TAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAAC GTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTT GCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCG CATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCA GTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGA TCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACA TGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAA CTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAA CGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTG AACGTTGTAAGTAAAGTTGGCAAGAAA
[0269] SEQ ID NO: 113 (Sítio de ligação ao ribossomo RBS2 e quadro de leitura aberto Of_oxc):
GCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATA TAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTT GATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCA TTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTAC AGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACAT CGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAAC GGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTT GAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAA GAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTAT CAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTG TATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGT CAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCC AGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTG ATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACG CACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATT CCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCC GCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGA TCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGG CGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACA GCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTC CCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACC GGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGA TGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTT GTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGC TAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAAC GTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTT GCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCG CATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCA GTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGA TCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACA TGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAA CTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAA CGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTG AACGTTGTAAGTAAAGTTGGCAAGAAA
[0270] SEQ ID NO: 114 (Sítio de ligação ao ribossomo RBS3 e quadro de leitura aberto Of_oxc):
GCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATA TAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTT GATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCA TTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTAC AGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACAT CGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAAC GGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTT GAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAA GAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTAT CAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTG TATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGT CAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCC AGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTG ATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACG CACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATT CCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCC GCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGA TCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGG CGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACA GCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTC CCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACC GGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGA TGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTT GTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGC TAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAAC GTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTT GCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCG CATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCA GTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGA TCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACA TGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAA CTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAA CGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTG AACGTTGTAAGTAAAGTTGGCAAGAAA
[0271] SEQ ID NO: 115 (Sítio de ligação ao ribossomo RBS4 e quadro de leitura aberto Of_oxc):
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAA TTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTT GATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCA TTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTAC AGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACAT CGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAAC GGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTT GAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAA GAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTAT CAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTG TATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGT CAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCC AGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTG ATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACG CACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATT CCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCC GCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGA TCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGG CGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACA GCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTC CCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACC GGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGA TGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTT GTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGC TAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAAC GTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTT GCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCG CATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCA GTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGA TCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACA TGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAA CTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAA CGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTG AACGTTGTAAGTAAAGTTGGCAAGAAA
[0272] SEQ ID NO: 116 (Sítio de ligação ao ribossomo RBS5 e quadro de leitura aberto Of_oxc):
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCGATCCTATAAAAAATA TAACAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTT GATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCA TTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTAC AGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACAT CGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAAC GGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTT GAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAA GAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTAT CAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTG TATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGT CAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCC AGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTG ATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACG CACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATT CCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCC GCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGA TCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGG CGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACA GCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTC CCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACC GGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGA TGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTT GTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGC TAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAAC GTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTT GCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCG CATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCA GTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGA TCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACA TGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAA CTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAA CGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTG AACGTTGTAAGTAAAGTTGGCAAGAAA
[0273] SEQ ID NO: 117 (Sítio de ligação ao ribossomo RBS6 e quadro de leitura aberto Of_oxc):
GCATCATCTACCTTTATTATTAATCAATCCATCTCAGTAAGTTTAAAATA TAACAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTT GATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCA TTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTAC AGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACAT CGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAAC GGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTT GAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAA GAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTAT CAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTG TATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGT CAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCC AGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTG ATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACG CACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATT CCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCC GCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGA TCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGG CGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACA GCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTC CCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACC GGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGA TGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTT GTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGC TAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAAC GTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTT GCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCG CATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCA GTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGA TCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACA TGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAA CTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAA CGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTG AACGTTGTAAGTAAAGTTGGCAAGAAA
[0274] SEQ ID NO: 118 (Sítio de ligação ao ribossomo RBS7 e quadro de leitura aberto Of_oxc):
GCATCAACTACCTCTATTAATAATCAATCCACATCTTGATCGTAAAAAA TTTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTT TGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGC ATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTA CAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACA TCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAA CGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGT TGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGA AGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTA TCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCT GTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCG GTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGAT CCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACC TGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATAC GCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCAT TCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATC CGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTG ATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGG GCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGA CAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTT CCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGAC CGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAG ATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGT TGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCG CTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAA CGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCG TTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAG CGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGC CAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCA GATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACG ACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCA GAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGA TCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAG CCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
[0275] SEQ ID NO: 119 (Sítio de ligação ao ribossomo RBS8 e quadro de leitura aberto Of_oxc):
GCATCACCTACCTAAATTACTAAACAAACCACCTGCATTTGTTTTAAAA ATTACAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTT TGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGC ATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTA CAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACA TCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAA CGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGT TGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGA AGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTA TCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCT GTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCG GTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGAT CCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACC TGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATAC GCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCAT TCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATC CGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTG ATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGG GCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGA CAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTT CCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGAC CGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAG ATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGT TGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCG CTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAA CGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCG TTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAG CGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGC CAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCA GATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACG ACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCA GAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGA TCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAG CCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
[0276] SEQ ID NO: 120 (Sítio de ligação ao ribossomo RBS1 e quadro de leitura aberto Sc_YEL020C):
GCATCAACTACCTAAATTAATCATCAAACCAACTAGAATATGAAAAAAA ATAAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAAT ATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCG GATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGC TGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGT TACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAAC TCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAG TGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCC CATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTC AGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGAC GTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTAC AGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGT CGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTC TGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTA GTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTC CCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATA GCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGT ACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCC GAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGG AGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATT CCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTG AATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTA CAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATAC TTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACA GACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATT GGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGG TGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACG GCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTAT TTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCA AGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTT GTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTC TCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAAC AAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
[0277] SEQ ID NO: 121 (Sítio de ligação ao ribossomo RBS2 e quadro de leitura aberto Sc_YEL020C):
GCATCAACTACCTCAATTAATAAACAAACCAACTGTCCAATGTATAAAA TTAAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAAT ATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCG GATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGC TGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGT TACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAAC TCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAG TGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCC CATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTC AGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGAC GTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTAC AGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGT CGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTC TGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTA GTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTC CCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATA GCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGT ACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCC GAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGG AGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATT CCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTG AATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTA CAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATAC TTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACA GACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATT GGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGG TGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACG GCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTAT TTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCA AGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTT GTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTC TCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAAC AAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
[0278] SEQ ID NO: 122 (Sítio de ligação ao ribossomo RBS3 e quadro de leitura aberto Sc_YEL020C):
GCATCAACTACCTCAATTACTAAACAATCCACATTATTAATATAAAAATA TAAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATA TGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGG ATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCT GCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTT ACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACT CAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGT GATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCC ATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCA GAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACG TCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACA GGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTC GAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCT GATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAG TAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCC CAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAG CGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTA CTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCG AAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGA GATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTC CTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGA ATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTAC AACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTT GTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGA CGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGG GCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTG CTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGC AGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTT ACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAA GAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTG TCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCT CGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACA AAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
[0279] SEQ ID NO: 123 (Sítio de ligação ao ribossomo RBS4 e quadro de leitura aberto Sc_YEL020C):
GCATCAACACCTAAATTAATTAACACACCATATATTAAACCATAAAAAA TAAGAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATA TGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGG ATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCT GCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTT ACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACT CAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGT GATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCC ATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCA GAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACG TCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACA GGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTC GAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCT GATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAG TAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCC CAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAG CGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTA CTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCG AAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGA GATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTC CTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGA ATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTAC AACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTT GTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGA CGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGG GCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTG CTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGC AGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTT ACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAA GAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTG TCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCT CGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACA AAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
[0280] SEQ ID NO: 124 (Sítio de ligação ao ribossomo RBS5 e quadro de leitura aberto Sc_YEL020C):
GCATCTTCTACCCATATTCCCCCTAATCCCATCTGTTATATTATTAAATT TAATAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATA TGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGG ATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCT GCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTT ACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACT CAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGT GATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCC ATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCA GAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACG TCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACA GGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTC GAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCT GATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAG TAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCC CAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAG CGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTA CTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCG AAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGA GATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTC CTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGA ATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTAC AACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTT GTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGA CGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGG GCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTG CTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGC AGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTT ACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAA GAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTG TCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCT CGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACA AAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
[0281] SEQ ID NO: 125 (Sítio de ligação ao ribossomo RBS6 e quadro de leitura aberto Sc_YEL020C):
GCATCATCTACCTACATTATTTAACAAACCACATTTAAAAGGAAAAAAT ATTAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAAT ATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCG GATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGC TGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGT TACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAAC TCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAG TGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCC CATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTC AGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGAC GTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTAC AGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGT CGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTC TGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTA GTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTC CCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATA GCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGT ACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCC GAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGG AGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATT CCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTG AATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTA CAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATAC TTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACA GACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATT GGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGG TGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACG GCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTAT TTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCA AGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTT GTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTC TCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAAC AAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
[0282] SEQ ID NO: 126 (Sítio de ligação ao ribossomo RBS7 e quadro de leitura aberto Sc_YEL020C):
GCATCACCTACCTATATTATTTATCATTCCACATGCGTTATTATTAAATT TTAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATA TGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGG ATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCT GCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTT ACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACT CAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGT GATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCC ATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCA GAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACG TCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACA GGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTC GAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCT GATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAG TAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCC CAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAG CGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTA CTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCG AAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGA GATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTC CTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGA ATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTAC AACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTT GTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGA CGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGG GCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTG CTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGC AGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTT ACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAA GAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTG TCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCT CGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACA AAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
[0283] SEQ ID NO: 127 (Sítio de ligação ao ribossomo RBS8 e quadro de leitura aberto Sc_YEL020C):
GCATCTAAACAACATATTCCCCCTAAACCAAAATATTTAGGGTATAAAA ATTAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAAT ATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCG GATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGC TGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGT TACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAAC TCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAG TGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCC CATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTC AGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGAC GTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTAC AGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGT CGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTC TGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTA GTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTC CCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATA GCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGT ACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCC GAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGG AGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATT CCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTG AATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTA CAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATAC TTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACA GACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATT GGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGG TGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACG GCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTAT TTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCA AGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTT GTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTC TCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAAC AAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
[0284] SEQ ID NO: 128 (Sítio de ligação ao ribossomo RBS1 e quadro de leitura aberto Ec_yfdE):
GCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAAT TTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTAT CGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATAT GGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGC ACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCAT GGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTT ATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACA ATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCT CATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTC CTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGG ATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCG GCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAG AGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTT TTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGT CTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGA TAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCA GGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTT TACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAA CGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGT CGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
[0285] SEQ ID NO: 129 (Sítio de ligação ao ribossomo RBS2 e quadro de leitura aberto Ec_yfdE):
GCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAAT ATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTA TCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATA TGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCG CACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCA TGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATT TATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTAC AATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCC TCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCT CCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAG GATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGC GGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAA GAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGT TTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACG TCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGG ATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCC AGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATT TTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAA ACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCG TCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
[0286] SEQ ID NO: 130 (Sítio de ligação ao ribossomo RBS3 e quadro de leitura aberto Ec_yfdE):
GCATCACTATTACAAAAAAAACACCAATATTACATGGTCTCTTTTAAAT ATTATAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTA TCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATA TGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCG CACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCA TGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATT TATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTAC AATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCC TCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCT CCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAG GATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGC GGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAA GAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGT TTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACG TCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGG ATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCC AGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATT TTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAA ACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCG TCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
[0287] SEQ ID NO: 131 (Sítio de ligação ao ribossomo RBS4 e quadro de leitura aberto Ec_yfdE):
GCATCATCTACCTATATTACTAAACAAACCAATTATAATAATTTTAAAAA TAAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTAT CGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATAT GGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGC ACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCAT GGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTT ATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACA ATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCT CATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTC CTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGG ATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCG GCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAG AGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTT TTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGT CTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGA TAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCA GGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTT TACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAA CGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGT CGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
[0288] SEQ ID NO: 132 (Sítio de ligação ao ribossomo RBS5 e quadro de leitura aberto Ec_yfdE):
GCATCACCTACCTCTATTACTAATCAAACCATTTAAATGTAGTAAAAAT ATAATAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTA TCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATA TGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCG CACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCA TGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATT TATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTAC AATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCC TCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCT CCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAG GATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGC GGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAA GAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGT TTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACG TCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGG ATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCC AGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATT TTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAA ACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCG TCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
[0289] SEQ ID NO: 133 (Sítio de ligação ao ribossomo RBS6 e quadro de leitura aberto Ec_yfdE):
GCATCAACTACCTAAATTAATAAACACACCAACTTCCATGAATAAAAAT ATAAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTA TCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATA TGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCG CACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCA TGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATT TATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTAC AATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCC TCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCT CCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAG GATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGC GGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAA GAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGT TTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACG TCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGG ATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCC AGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATT TTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAA ACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCG TCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
[0290] SEQ ID NO: 134 (Sítio de ligação ao ribossomo RBS7 e quadro de leitura aberto Ec_yfdE):
GCATCATCTACCTATATTATTTACCACCCCACCTAACTTTAGATTAAAA TTTACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTA TCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATA TGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCG CACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCA TGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATT TATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTAC AATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCC TCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCT CCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAG GATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGC GGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAA GAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGT TTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACG TCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGG ATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCC AGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATT TTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAA ACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCG TCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
[0291] SEQ ID NO: 135 (Sítio de ligação ao ribossomo RBS8 e quadro de leitura aberto Ec_yfdE):
GCATCACCTACCTTCATTACTCACCATACCAACTAGCACAGTTATAAAA ATAAGAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTA TCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATA TGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCG CACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCA TGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATT TATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTAC AATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCC TCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCT CCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAG GATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGC GGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAA GAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGT TTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACG TCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGG ATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCC AGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATT TTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAA ACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCG TCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCG AGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAA AATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGA CCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
[0292] SEQ ID NO: 136 (Sítio de ligação ao ribossomo RBS1 e quadro de leitura aberto Ec_frc):
GCATCATCTACCTAAATTAATAAACAACCCATTTATAAACCCTTTAAAAT TAACAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGT GCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTA TTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGA TATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTC TATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGA TCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCAC ATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGG TTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATG AAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGA TGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAAT GCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGC GTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCG CGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAAT ACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAA TGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
[0293] SEQ ID NO: 137 (Sítio de ligação ao ribossomo RBS2 e quadro de leitura aberto Ec_frc):
GCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAA TAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGT GCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTA TTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGA TATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTC TATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGA TCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCAC ATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGG TTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATG AAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGA TGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAAT GCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGC GTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCG CGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAAT ACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAA TGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
[0294] SEQ ID NO: 138 (Sítio de ligação ao ribossomo RBS3 e quadro de leitura aberto Ec_frc):
GCATCATCTACCTTAATTAATAAACAAACCAATTTAACTAATTTAAAAAA TATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTG CAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTAT TAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGAT ATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCT ATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGAT CCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACA TGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGT TCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGA AAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGAT GGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATG CATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCG TGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGC GTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATA CCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAAT GCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
[0295] SEQ ID NO: 139 (Sítio de ligação ao ribossomo RBS4 e quadro de leitura aberto Ec_frc):
GCATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATA TAAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGT GCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTA TTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGA TATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTC TATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGA TCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCAC ATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGG TTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATG AAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGA TGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAAT GCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGC GTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCG CGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAAT ACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAA TGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
[0296] SEQ ID NO: 140 (Sítio de ligação ao ribossomo RBS5 e quadro de leitura aberto Ec_frc):
GCATCTTAATCAATTTTAAACTCACCAAATCAAATATCACGTAAAAAATA TAAGAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGT GCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTA TTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGA TATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTC TATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGA TCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCAC ATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGG TTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATG AAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGA TGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAAT GCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGC GTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCG CGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAAT ACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAA TGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAA ACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAAC ACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTAC AGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATA CACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATAT TCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCG CCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGA CCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCG CCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCG ACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
[0297] SEQ ID NO: 141 (Sítio de ligação ao ribossomo RBS6 e quadro de leitura aberto Ec_frc):
GCATCAACTACCTCAATTATTTAACACACCAATTACTATACGTAAAAAT ATAAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTG TGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTT ATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAG ATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTT CTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTG ATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCA CATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTG GTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTAT GAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGG GATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGA ATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGG GCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGC CGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGA ATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGT AATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGG AAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAA ACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGT ACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAA TACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGAT ATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTG CGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCT GACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTG CGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAG CGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
[0298] SEQ ID NO: 142 (Sítio de ligação ao ribossomo RBS7 e quadro de leitura aberto Ec_frc):
GCATCTTAATCAATTTTAAACTCACCAAATCAAAAAAATGCGTATAAAA ATTAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTG TGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTT ATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAG ATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTT CTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTG ATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCA CATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTG GTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTAT GAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGG GATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGA ATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGG GCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGC CGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGA ATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGT AATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGG AAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAA ACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGT ACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAA TACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGAT ATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTG CGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCT GACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTG CGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAG CGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
[0299] SEQ ID NO: 143 (Sítio de ligação ao ribossomo RBS8 e quadro de leitura aberto Ec_frc):
GCATCTTAATCAATTTTAAACTCACCAAATCAAAGGCCGACTTAAAAAT ATTAGAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTG TGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTT ATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAG ATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTT CTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTG ATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCA CATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTG GTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTAT GAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGG GATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGA ATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGG GCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGC CGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGA ATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGT AATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGG AAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAA ACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGT ACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAA TACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGAT ATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTG CGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCT GACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTG CGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAG CGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
[0300] SEQ ID NO: 144 (Sítio de ligação ao ribossomo RBS1 e quadro de leitura aberto Of_oxlT):
GCATCACCTAAAAATTATAAATAAATTAATAAACCTTTTAACATAAAAAT TAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATC GTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTC CAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTC TTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTT CTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTG ATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTG ACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGT ATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAAC GCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCC GTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCAT TCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTA TCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAG GATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGAC CGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCC CTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTC GATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCG ATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTT GTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTC TTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTG CAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTT CTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCA GTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
[0301] SEQ ID NO: 145 (Sítio de ligação ao ribossomo RBS2 e quadro de leitura aberto Of_oxlT):
GCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAA TTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATC GTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTC CAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTC TTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTT CTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTG ATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTG ACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGT ATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAAC GCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCC GTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCAT TCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTA TCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAG GATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGAC CGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCC CTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTC GATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCG ATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTT GTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTC TTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTG CAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTT CTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCA GTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
[0302] SEQ ID NO: 146 (Sítio de ligação ao ribossomo RBS3 e quadro de leitura aberto Of_oxlT):
GCATCACCTAAAAATTATAAATAAATTAATAAACATAACATAAATAAAAT TAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATC GTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTC CAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTC TTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTT CTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTG ATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTG ACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGT ATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAAC GCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCC GTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCAT TCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTA TCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAG GATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGAC CGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCC CTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTC GATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCG ATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTT GTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTC TTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTG CAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTT CTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCA GTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
[0303] SEQ ID NO: 147 (Sítio de ligação ao ribossomo RBS4 e quadro de leitura aberto Of_oxlT):
GCATCACCTAAAAATTATAAATAAATTAATAAACCACAATACTAAAAATT TAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATC GTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTC CAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTC TTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTT CTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTG ATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTG ACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGT ATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAAC GCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCC GTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCAT TCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTA TCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAG GATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGAC CGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCC CTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTC GATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCG ATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTT GTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTC TTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTG CAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTT CTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCA GTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
[0304] SEQ ID NO: 148 (Sítio de ligação ao ribossomo RBS5 e quadro de leitura aberto Of_oxlT):
GCATCAACTACCTACATTAATAACCAACCCAAATGCCTAATCAATAAAT ATTATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAAT CGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGT CCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTT CTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGT TCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATT GATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTT GACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGG TATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAAC GCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCC GTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCAT TCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTA TCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAG GATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGAC CGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCC CTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTC GATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCG ATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTT GTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTC TTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTG CAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTT CTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCA GTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
[0305] SEQ ID NO: 149 (Sítio de ligação ao ribossomo RBS6 e quadro de leitura aberto Of_oxlT):
GCATCATCTACCTATATTATTCATCAAACCATTTGATCCAACATTAAATA TTAAAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATC GTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTC CAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTC TTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTT CTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTG ATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTG ACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGT ATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAAC GCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCC GTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCAT TCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTA TCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAG GATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGAC CGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCC CTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTC GATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCG ATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTT GTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTC TTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTG CAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTT CTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCA GTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
[0306] SEQ ID NO: 150 (Sítio de ligação ao ribossomo RBS7 e quadro de leitura aberto Of_oxlT):
GCATCACCTACCTCAATTATTAATCATACCAACTGGATTTGATTTAAAA ATAAGAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAAT CGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGT CCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTT CTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGT TCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATT GATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTT GACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGG TATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAAC GCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCC GTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCAT TCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTA TCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAG GATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGAC CGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCC CTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTC GATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCG ATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTT GTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTC TTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTG CAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTT CTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCA GTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
[0307] SEQ ID NO: 151 (Sítio de ligação ao ribossomo RBS8 e quadro de leitura aberto Of_oxlT):
GCATCACCTACCTTTATTACTCATCATACCAAATGGTAGCGGATTAAAT ATTAGAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAAT CGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGT CCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTT CTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGT TCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATT GATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTT GACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGG TATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAAC GCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCC GTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCAT TCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTA TCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAG GATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGAC CGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCC CTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTC GATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCG ATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTT GTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGC CATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCAT CGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTC TTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTG CAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTT CTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCA GTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
[0308] SEQ ID NO: 152 (Sítio de ligação ao ribossomo 'RBS1 e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCATCTACCTTAATTAATCAACAAACCAATTACCAACCTAAAAAAT ATAAGAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAG TACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGC CGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAG CCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGT CCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATA ATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCA GTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTA AGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGAT AACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATA TCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGA TAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCA GACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAA GAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTA GACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAA GGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAG GCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGAT CCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATT TGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGAT TTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGC TCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAG GGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTG GAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGAC GACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTA GAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAAT GCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCC TGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTA TGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATG AATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCC GACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAG CTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGG CAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATC GAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGAC TG
[0309] SEQ ID NO: 153 (Sítio de ligação ao ribossomo RBS2 e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAA TAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
[0310] SEQ ID NO: 154 (Sítio de ligação ao ribossomo RBS3 e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATA TAATAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
[0311] SEQ ID NO: 155 (Sítio de ligação ao ribossomo RBS4 e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAA TAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
[0312] SEQ ID NO: 156 (Sítio de ligação ao ribossomo RBS5 e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCTACCCCTAACAACCCATAAATTTAACTTTTCTACAAAAAAAAAT ATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAG TACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGC CGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAG CCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGT CCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATA ATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCA GTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTA AGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGAT AACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATA TCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGA TAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCA GACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAA GAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTA GACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAA GGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAG GCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGAT CCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATT TGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGAT TTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGC TCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAG GGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTG GAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGAC GACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTA GAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAAT GCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCC TGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTA TGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATG AATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCC GACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAG CTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGG CAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATC GAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGAC TG
[0313] SEQ ID NO: 157 (Sítio de ligação ao ribossomo RBS6 e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCACCTACCTACATTAATAACCATACCATCTCAGTTTTAAATAAAT TTTAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAG TACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGC CGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAG CCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGT CCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATA ATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCA GTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTA AGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGAT AACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATA TCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGA TAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCA GACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAA GAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTA GACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAA GGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAG GCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGAT CCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATT TGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGAT TTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGC TCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAG GGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTG GAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGAC GACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTA GAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAAT GCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCC TGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTA TGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATG AATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCC GACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAG CTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGG CAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATC GAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGAC TG
[0314] SEQ ID NO: 158 (Sítio de ligação ao ribossomo RBS7 e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCACCTACCTTAATTAATCACCATTCCATATCCTTTCAAAAAAAAT ATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAG TACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGC CGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAG CCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGT CCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATA ATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCA GTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTA AGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGAT AACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATA TCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGA TAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCA GACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAA GAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTA GACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAA GGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAG GCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGAT CCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATT TGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGAT TTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGC TCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAG GGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTG GAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGAC GACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTA GAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAAT GCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCC TGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTA TGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATG AATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCC GACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAG CTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGG CAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATC GAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGAC TG
[0315] SEQ ID NO: 159 (Sítio de ligação ao ribossomo RBS8 e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCACCTACCTCTATTAATCATCATTCCAATTTAGGCTCATTAAATA TTAGAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
[0316] SEQ ID NO: 160 (Sítio de ligação ao ribossomo RBS1b e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCATCTACCTTAATTAATCAACAAACCAATTACCAACCTAAAAAAT ATAAGAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAG TACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGC CGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAG CCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGT CCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATA ATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCA GTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTA AGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGAT AACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATA TCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGA TAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCA GACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAA GAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTA GACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAA GGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAG GCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGAT CCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATT TGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGAT TTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGC TCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAG GGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTG GAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGAC GACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTA GAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAAT GCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCC TGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTA TGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATG AATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCC GACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAG CTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGG CAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATC GAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGAC TG
[0317] SEQ ID NO: 161 (Sítio de ligação ao ribossomo RBS2b e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCAACTACCTTTATTATTCAACAAACCAATTTAACATAGTAAAAAAA TAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
[0318] SEQ ID NO: 162 (Sítio de ligação ao ribossomo RBS3b e quadro de leitura aberto Sc_YEL020C (códon otimizado)):
GCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATA TAAGAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGT ACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCC GACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGC CGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTC CTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAA TAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAG TCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAA GTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATA ACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATAT CGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGAT AGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAG ACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAG AACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAG ACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAG GCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGC TCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCC TTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTG ACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTT AAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTC ACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGG AAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGA GCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGA CTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAA TAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCT ACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGA GTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGG AGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAAT AACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGA CCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCT AACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
[0319] SEQ ID NO: 163 (Oxalil-CoA decarboxilase (OXC) de Saccharomyces cerevisiae S288C; EC 4.1.1.8; Acesso UniProt P39994; Sc_YEL020C; Códon otimizado):
ATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTAC GGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGA CACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCG CAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCT GTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATA GTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTC AGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGT CCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAAC CCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCG ATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAG AACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGAC CCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAA CATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGAC GCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGC ATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTC TTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTT CACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGA CAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTA AGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCA CGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGA AAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAG CTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGAC TATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAAT AAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTA CAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAG TTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGA GATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATA ACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGAC CGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTA ACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCA GTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGA ACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
[0320] SEQ ID NO: 164 (Sítio de ligação ao ribossomo):
GCATCTAAACTAAAATACAATAACCAAAAATCATTACAAATGAAAAAAA ATAATAAA
[0321] SEQ ID NO: 165 (Sítio de ligação ao ribossomo):
GCATCTAAACTAAAATACAATAACCAAAAATCATGTTTAACAAATAAAA ATAATAAA
[0322] SEQ ID NO: 166 (Sítio de ligação ao ribossomo):
GCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAAAAAA ATTATAAA
[0323] SEQ ID NO: 167 (Sítio de ligação ao ribossomo):
GCATCTAAACTAAAATACAATAACCAAAAATCATACTACATGTATAAAA TTAAAAAA
[0324] SEQ ID NO: 168 (Sítio de ligação ao ribossomo):
GCATCTAAACTAAAATACAATAACCAAAAATCATTTTCACAATATAAAAA TAACAAA
[0325] SEQ ID NO: 169 (Sítio de ligação ao ribossomo):
GCATCATCTACCTCAATTATTCACCATTCCATCTGTCTAGCCAAAAAAT TTAAAAAA
[0326] SEQ ID NO: 170 (Sítio de ligação ao ribossomo):
GCATCATCTACCTAAATTACTCATCATCCCATTTTAGTCAGCTTAAAAT ATTATAAA
[0327] SEQ ID NO: 171 (Sítio de ligação ao ribossomo):
GCATCATCTACCTATATTATTTATCATTCCATATCATGCGTGAATAAAAA TAATAAA
[0328] SEQ ID NO: 172 (Sítio de ligação ao ribossomo):
GCATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAAT TAACAAA
[0329] SEQ ID NO: 173 (Sítio de ligação ao ribossomo):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAA ATTATAAA
[0330] SEQ ID NO: 174 (Sítio de ligação ao ribossomo):
GCATCAACTACCTCAATTAATAATCAAACCATCTGAACAATGAATAAAT TTAAAAAA
[0331] SEQ ID NO: 175 (Sítio de ligação ao ribossomo):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAGATATAAAA TTAACAAA
[0332] SEQ ID NO: 176 (Sítio de ligação ao ribossomo):
GCATCAACTACCTTAATTATTCATCAAACCATATAACATACTAAAAAAAA TAACAAA
[0333] SEQ ID NO: 177 (Sítio de ligação ao ribossomo):
GCATCATCTACCTATATTACTAATCATTCCAATTAATTACCTTATAAAAT TAAGAAA
[0334] SEQ ID NO: 178 (Sítio de ligação ao ribossomo):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCCCCCCACGTTTAAAA TTAAAAAA
[0335] SEQ ID NO: 179 (Sítio de ligação ao ribossomo):
GCATCAACTACCTTCATTACTTAACAATCCACCTGTTGTGAGTAAAAAT ATAAGAAA
[0336] SEQ ID NO: 180 (Sítio de ligação ao ribossomo):
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCCCTAACCCTTAAAATA TAATAAA
[0337] SEQ ID NO: 181 (Sítio de ligação ao ribossomo):
GCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATA TAATAAA
[0338] SEQ ID NO: 182 (Sítio de ligação ao ribossomo):
GCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATA TAATAAA
[0339] SEQ ID NO: 183 (Sítio de ligação ao ribossomo):
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAA TTAAAAA
[0340] SEQ ID NO: 184 (Sítio de ligação ao ribossomo):
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCGATCCTATAAAAAATA TAACAAA
[0341] SEQ ID NO: 185 (Sítio de ligação ao ribossomo):
GCATCATCTACCTTTATTATTAATCAATCCATCTCAGTAAGTTTAAAATA TAACAAA
[0342] SEQ ID NO: 186 (Sítio de ligação ao ribossomo):
GCATCAACTACCTCTATTAATAATCAATCCACATCTTGATCGTAAAAAA TTTAAAAA
[0343] SEQ ID NO: 187 (Sítio de ligação ao ribossomo):
GCATCACCTACCTAAATTACTAAACAAACCACCTGCATTTGTTTTAAAA ATTACAAA
[0344] SEQ ID NO: 188 (Sítio de ligação ao ribossomo):
GCATCAACTACCTAAATTAATCATCAAACCAACTAGAATATGAAAAAAA ATAAAAAA
[0345] SEQ ID NO: 189 (Sítio de ligação ao ribossomo):
GCATCAACTACCTCAATTAATAAACAAACCAACTGTCCAATGTATAAAA TTAAAAAA
[0346] SEQ ID NO: 190 (Sítio de ligação ao ribossomo):
GCATCAACTACCTCAATTACTAAACAATCCACATTATTAATATAAAAATA TAAAAAA
[0347] SEQ ID NO: 191 (Sítio de ligação ao ribossomo):
GCATCAACACCTAAATTAATTAACACACCATATATTAAACCATAAAAAA TAAGAAA
[0348] SEQ ID NO: 192 (Sítio de ligação ao ribossomo):
GCATCTTCTACCCATATTCCCCCTAATCCCATCTGTTATATTATTAAATT TAATAAA
[0349] SEQ ID NO: 193 (Sítio de ligação ao ribossomo):
GCATCATCTACCTACATTATTTAACAAACCACATTTAAAAGGAAAAAAT ATTAAAAA
[0350] SEQ ID NO: 194 (Sítio de ligação ao ribossomo):
GCATCACCTACCTATATTATTTATCATTCCACATGCGTTATTATTAAATT TTAAAAA
[0351] SEQ ID NO: 195 (Sítio de ligação ao ribossomo):
GCATCTAAACAACATATTCCCCCTAAACCAAAATATTTAGGGTATAAAA ATTAAAAA
[0352] SEQ ID NO: 196 (Sítio de ligação ao ribossomo):
GCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAAT TTAAAAA
[0353] SEQ ID NO: 197 (Sítio de ligação ao ribossomo):
GCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAAT ATAACAAA
[0354] SEQ ID NO: 198 (Sítio de ligação ao ribossomo):
GCATCACTATTACAAAAAAAACACCAATATTACATGGTCTCTTTTAAAT ATTATAAA
[0355] SEQ ID NO: 199 (Sítio de ligação ao ribossomo):
GCATCATCTACCTATATTACTAAACAAACCAATTATAATAATTTTAAAAA TAAAAAA
[0356] SEQ ID NO: 200 (Sítio de ligação ao ribossomo):
GCATCACCTACCTCTATTACTAATCAAACCATTTAAATGTAGTAAAAAT ATAATAAA
[0357] SEQ ID NO: 201 (Sítio de ligação ao ribossomo):
GCATCAACTACCTAAATTAATAAACACACCAACTTCCATGAATAAAAAT ATAAAAAA
[0358] SEQ ID NO: 202 (Sítio de ligação ao ribossomo):
GCATCATCTACCTATATTATTTACCACCCCACCTAACTTTAGATTAAAA TTTACAAA
[0359] SEQ ID NO: 203 (Sítio de ligação ao ribossomo):
GCATCACCTACCTTCATTACTCACCATACCAACTAGCACAGTTATAAAA ATAAGAAA
[0360] SEQ ID NO: 204 (Sítio de ligação ao ribossomo):
GCATCATCTACCTAAATTAATAAACAACCCATTTATAAACCCTTTAAAAT TAACAAA
[0361] SEQ ID NO: 205 (Sítio de ligação ao ribossomo):
GCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAA TAATAAA
[0362] SEQ ID NO: 206 (Sítio de ligação ao ribossomo):
GCATCATCTACCTTAATTAATAAACAAACCAATTTAACTAATTTAAAAAA TATAAA
[0363] SEQ ID NO: 207 (Sítio de ligação ao ribossomo):
GCATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATA TAAAAAA
[0364] SEQ ID NO: 208 (Sítio de ligação ao ribossomo):
GCATCTTAATCAATTTTAAACTCACCAAATCAAATATCACGTAAAAAATA TAAGAAA
[0365] SEQ ID NO: 209 (Sítio de ligação ao ribossomo):
GCATCAACTACCTCAATTATTTAACACACCAATTACTATACGTAAAAAT ATAAAAAA
[0366] SEQ ID NO: 210 (Sítio de ligação ao ribossomo):
GCATCTTAATCAATTTTAAACTCACCAAATCAAAAAAATGCGTATAAAA ATTAAAAA
[0367] SEQ ID NO: 211 (Sítio de ligação ao ribossomo):
GCATCTTAATCAATTTTAAACTCACCAAATCAAAGGCCGACTTAAAAAT ATTAGAAA
[0368] SEQ ID NO: 212 (Sítio de ligação ao ribossomo):
GCATCACCTAAAAATTATAAATAAATTAATAAACCTTTTAACATAAAAAT TAATAAA
[0369] SEQ ID NO: 213 (Sítio de ligação ao ribossomo):
GCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAA TTACAAA
[0370] SEQ ID NO: 214 (Sítio de ligação ao ribossomo):
GCATCACCTAAAAATTATAAATAAATTAATAAACATAACATAAATAAAAT TAATAAA
[0371] SEQ ID NO: 215 (Sítio de ligação ao ribossomo):
GCATCACCTAAAAATTATAAATAAATTAATAAACCACAATACTAAAAATT TAATAAA
[0372] SEQ ID NO: 216 (Sítio de ligação ao ribossomo):
GCATCAACTACCTACATTAATAACCAACCCAAATGCCTAATCAATAAAT ATTATAAA
[0373] SEQ ID NO: 217 (Sítio de ligação ao ribossomo):
GCATCATCTACCTATATTATTCATCAAACCATTTGATCCAACATTAAATA TTAAAAA
[0374] SEQ ID NO: 218 (Sítio de ligação ao ribossomo):
GCATCACCTACCTCAATTATTAATCATACCAACTGGATTTGATTTAAAA ATAAGAAA
[0375] SEQ ID NO: 219 (Sítio de ligação ao ribossomo):
GCATCACCTACCTTTATTACTCATCATACCAAATGGTAGCGGATTAAAT ATTAGAAA
[0376] SEQ ID NO: 220 (Sítio de ligação ao ribossomo):
GCATCATCTACCTTAATTAATCAACAAACCAATTACCAACCTAAAAAAT ATAAGAAA
[0377] SEQ ID NO: 221 (Sítio de ligação ao ribossomo):
GCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAA TAAAAAA
[0378] SEQ ID NO: 222 (Sítio de ligação ao ribossomo):
GCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATA TAATAAA
[0379] SEQ ID NO: 223 (Sítio de ligação ao ribossomo):
GCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAA TAAAAAA
[0380] SEQ ID NO: 224 (Sítio de ligação ao ribossomo):
GCATCTACCCCTAACAACCCATAAATTTAACTTTTCTACAAAAAAAAAT ATAAAAAA
[0381] SEQ ID NO: 225 (Sítio de ligação ao ribossomo):
GCATCACCTACCTACATTAATAACCATACCATCTCAGTTTTAAATAAAT TTTAAAAA
[0382] SEQ ID NO: 226 (Sítio de ligação ao ribossomo):
GCATCACCTACCTTAATTAATCACCATTCCATATCCTTTCAAAAAAAAT ATAAAAAA
[0383] SEQ ID NO: 227 (Sítio de ligação ao ribossomo):
GCATCACCTACCTCTATTAATCATCATTCCAATTTAGGCTCATTAAATA TTAGAAA
[0384] SEQ ID NO: 228 (Sítio de ligação ao ribossomo):
GCATCATCTACCTTAATTAATCAACAAACCAATTACCAACCTAAAAAAT ATAAGAAA
[0385] SEQ ID NO: 229 (Sítio de ligação ao ribossomo):
GCATCAACTACCTTTATTATTCAACAAACCAATTTAACATAGTAAAAAAA TAAAAAA
[0386] SEQ ID NO: 230 (Sítio de ligação ao ribossomo):
GCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATA TAAGAAA

Claims (64)

REIVINDICAÇÕES
1. Bactéria comensal, CARACTERIZADA pelo fato de que foi modificada para ter atividade degradante de oxalato aumentada em relação a uma bactéria similar ou de outro modo idêntica que não foi modificada.
2. Bactéria, de acordo com a reivindicação 1, CARACTERIZADA pelo fato de que a bactéria é de um gênero selecionado do grupo que consiste em Bacteroides, Alistipes, Faecalibacterium, Parabacteroides, Prevotella, Roseburia, Ruminococcus, Clostridium, Oscillibacter, Gemmiger, Barnesiella, Dialister, Parasutterella, Phascolarctobacterium, Propionibacterium, Sutterella, Blautia, Paraprevotella, Coprococcus, Odoribacter, Spiroplasma, Anaerostipes e Akkermansia.
3. Bactéria, de acordo com a reivindicação 2, CARACTERIZADA pelo fato de que o gênero é Bacteroides.
4. Bactéria, de acordo com qualquer uma das reivindicações 1 a 3, CARACTERIZADA pelo fato de que a bactéria similar ou de outro modo idêntica que não foi modificada não tem atividade degradante de oxalato detectável.
5. Bactéria, de acordo com qualquer uma das reivindicações 1 a 4, CARACTERIZADA pelo fato de que a bactéria similar ou de outro modo idêntica que não foi modificada não tem genes conhecidos por estarem envolvidos no catabolismo de oxalato.
6. Bactéria, de acordo com qualquer uma das reivindicações 1 a 5, CARACTERIZADA pelo fato de que nenhum membro que ocorre naturalmente do mesmo gênero como a bactéria tem atividade degradante de oxalato detectável.
7. Bactéria, de acordo com qualquer uma das reivindicações 1 a 6, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma proteína, ou um fragmento funcional ou variante da mesma, selecionada do grupo que consiste em: (i) uma oxalato:formiato antiportadora (OxlT);
(ii) uma oxalato descarboxilase (OXDC)- EC 4.1.1.2; (iii) uma oxalato oxidase (OXO)- EC 1.2.3.4; (iv) uma oxalato óxido-redutase (OOR)- EC 1.2.7.10; (v) uma oxalato-CoA ligase/Oxalil-CoA sintetase (OXS)- EC 6.2.1.8; (vi) uma formil-CoA:oxalato CoA-transferase (FCOCT)- EC 2.8.3.16; (vii) uma acetil-CoA:oxalato CoA-transferase (ACOCT)- EC 2.8.3.19; (viii) uma succinil-CoA:oxalato CoA-transferase (SCOCT)- EC 2.8.3.2; (ix) uma oxalil-CoA descarboxilase (OXC)- EC 4.1.1.8; (x) uma oxalil-CoA redutase/glioxilato:NADP+ óxido-redutase (OXR)- EC
1.2.1.17; e (xi) uma combinação de qualquer uma das proteínas anteriores.
8. Bactéria, de acordo com a reivindicação 7, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma OXS ou um fragmento funcional ou variante da mesma, e uma OXC ou um fragmento funcional ou variante da mesma.
9. Bactéria, de acordo com a reivindicação 8, CARACTERIZADA pelo fato de que a bactéria compreende ainda um ou mais transgenes que codificam uma FCOCT ou um fragmento funcional ou variante da mesma.
10. Bactéria, de acordo com a reivindicação 8 ou 9, CARACTERIZADA pelo fato de que a bactéria compreende ainda um ou mais transgenes que codificam uma ACOCT ou um fragmento funcional ou variante da mesma.
11. Bactéria, de acordo com a reivindicação 7, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma OXS ou um fragmento funcional ou variante da mesma, uma primeira OXC ou um fragmento funcional ou variante da mesma, uma segunda OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma, e uma ACOCT ou um fragmento funcional ou variante da mesma.
12. Bactéria, de acordo com a reivindicação 7, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma primeira OXS ou um fragmento funcional ou variante da mesma, uma segunda OXS ou um fragmento funcional ou variante da mesma, uma OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma, e uma ACOCT ou um fragmento funcional ou variante da mesma.
13. Bactéria, de acordo com a reivindicação 7, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma OxIT ou um fragmento funcional ou variante da mesma, uma primeira OXS ou um fragmento funcional ou variante da mesma, uma segunda OXS ou um fragmento funcional ou variante da mesma, uma primeira OXC ou um fragmento funcional ou variante da mesma, uma segunda OXC ou um fragmento funcional ou variante da mesma, uma FCOCT ou um fragmento funcional ou variante da mesma, e uma ACOCT ou um fragmento funcional ou variante da mesma.
14. Bactéria, de acordo com qualquer uma das reivindicações 7 a 13, CARACTERIZADA pelo fato de que: (i) a OxIT é uma OxIT de O. formigenes; (ii) a OXS é uma OXS de S. cerevisiae ou A. thaliana; (iii) a OXC é uma OXC de S. cerevisiae ou O. formigenes; (iv) a FCOCT é uma FCOCT de E. coli; e/ou (v) a ACOCT é uma ACOCT de E. coli.
15. Bactéria, de acordo com a reivindicação 14, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma OXS de S. cerevisiae, uma OXC de S. cerevisiae e uma OxIT de O. formigenes.
16. Bactéria, de acordo com a reivindicação 14, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae e uma OxIT de O. formigenes.
17. Bactéria, de acordo com a reivindicação 14, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OxIT de O. formigenes.
18. Bactéria, de acordo com a reivindicação 14, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OXS de S. cerevisiae, uma OXC de S. cerevisiae, uma OXS de A. thaliana, uma ACOCT de E. coli e uma OxIT de O. formigenes.
19. Bactéria, de acordo com a reivindicação 14, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma FCOCT de E. coli, uma OXC de O. formigenes, uma OxIT de O. formigenes, uma ACOCT de E. coli e uma OXS de S. cerevisiae.
20. Bactéria, de acordo com a reivindicação 14, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma OxIT de O. formigenes, uma FCOCT de E. coli, uma OXC de S. cerevisiae, uma ACOCT de E. coli, uma OXS de S. cerevisiae e uma OXS de A. thaliana.
21. Bactéria, de acordo com a reivindicação 14, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que codificam uma OxIT de O. formigenes, uma FCOCT de E. coli, uma OXC de S. cerevisiae, uma ACOCT de E. coli e uma OXS de S. cerevisiae.
22. Bactéria, de acordo com qualquer uma das reivindicações 1 a 21, CARACTERIZADA pelo fato de que o um ou mais transgenes são em um plasmídeo, em um cromossomo artificial bacteriano ou são integrados genomicamente.
23. Bactéria, de acordo com qualquer uma das reivindicações 1 a 22, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID NOs: 1 a 31.
24. Bactéria, de acordo com a reivindicação 23, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21.
25. Bactéria, de acordo com a reivindicação 23, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21.
26. Bactéria, de acordo com a reivindicação 23, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21.
27. Bactéria, de acordo com a reivindicação 23, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 14, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO:
21.
28. Bactéria, de acordo com a reivindicação 23, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 25, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16.
29. Bactéria, de acordo com a reivindicação 23, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 14.
30. Bactéria, de acordo com a reivindicação 23, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 21, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 7, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 26 ou SEQ ID NO: 163, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 2 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 16.
31. Bactéria, de acordo com qualquer uma das reivindicações 7 a 30, CARACTERIZADA pelo fato de que pelo menos um dos um ou mais transgenes está operacionalmente ligado a um sítio de ligação ao ribossomo (RBS).
32. Bactéria, de acordo com a reivindicação 31, CARACTERIZADA pelo fato de que o RBS compreende a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 164 a 230.
33. Bactéria, de acordo com a reivindicação 32, CARACTERIZADA pelo fato de que: (i) a bactéria compreende um transgene que codifica uma OxIT de O. formigenes operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 212 a 219; (ii) a bactéria compreende um transgene que codifica uma OXS de S. cerevisiae operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 172 a 179; (iii) a bactéria compreende um transgene que codifica uma OXS de A. thaliana operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 164 a 171; (iv) a bactéria compreende um transgene que codifica uma OXC de S. cerevisiae operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 188 a 195 ou 220 a 230; (v) a bactéria compreende um transgene que codifica uma OXC de O. formigenes operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 180 a 187; (vi) a bactéria compreende um transgene que codifica uma FCOCT de E. coli operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 204 a 211; e/ou (vii) a bactéria compreende um transgene que codifica uma ACOCT de E. coli operacionalmente ligado a um RBS compreendendo a sequência de nucleotídeos de qualquer uma das SEQ ID NOs: 196 a 203.
34. Bactéria, de acordo com qualquer uma das reivindicações 1 a 33, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID NOs: 96 a 162:
35. Bactéria, de acordo com a reivindicação 34, CARACTERIZADA pelo fato de que (i) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 104; (ii) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 148, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (iii) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 144, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (iv) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 147, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 104, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 97, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 131, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 136 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113; (v) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO:
145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; (vi) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; (vii) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 112, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 99, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145; (viii) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID
NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 154, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 131, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 149; (ix) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 148, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (x) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (xi) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 128, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 144, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 115, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 98 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105; (xii) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 113 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; (xiii) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 137, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 153, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 105 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 96; ou (xiv) a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 139, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 129, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 155, uma sequência de nucleotídeos tendo pelo menos
80 % de identidade com a SEQ ID NO: 112, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 99, uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 106 e uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com a SEQ ID NO: 145.
36. Bactéria, de acordo com qualquer uma das reivindicações 1 a 35, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais ácidos nucleicos compreendendo uma sequência de nucleotídeos tendo pelo menos 80 % de identidade com qualquer uma das SEQ ID NOs: 82 a 95.
37. Bactéria, de acordo com qualquer uma das reivindicações 7 a 36, CARACTERIZADA pelo fato de que pelo menos um dos um ou mais transgenes está operacionalmente ligado a pelo menos um promotor constitutivo.
38. Bactéria, de acordo com a reivindicação 37, CARACTERIZADA pelo fato de que o promotor é um promotor derivado de fago.
39. Bactéria, de acordo com a reivindicação 37 ou 38, CARACTERIZADA pelo fato de que o promotor compreende a sequência consenso GTTAA(n)4-7GTTAA(n)34- 38TA(n)2TTTG (SEQ ID: 70).
40. Bactéria, de acordo com qualquer uma das reivindicações 37 a 39, CARACTERIZADA pelo fato de que o promotor compreende SEQ ID NO: 68, SEQ ID NO: 69 ou SEQ ID NO: 71.
41. Bactéria, de acordo com qualquer uma das reivindicações 1 a 40, CARACTERIZADA pelo fato de que a bactéria foi modificada para colonizar o intestino humano com maior abundância, estabilidade, previsibilidade ou facilidade de colonização inicial.
42. Bactéria, de acordo com a reivindicação 41, CARACTERIZADA pelo fato de que a bactéria foi modificada para aumentar sua capacidade de utilizar um nutriente privilegiado como fonte de carbono.
43. Bactéria, de acordo com a reivindicação 42, CARACTERIZADA pelo fato de que a bactéria compreende um ou mais transgenes que aumentam sua capacidade de utilizar um nutriente privilegiado como fonte de carbono.
44. Bactéria, de acordo com a reivindicação 42 ou 43, CARACTERIZADA pelo fato de que o nutriente privilegiado é um polissacarídeo marinho.
45. Bactéria, de acordo com a reivindicação 44, CARACTERIZADA pelo fato de que o polissacarídeo marinho é um porfirano.
46. Bactéria, de acordo com qualquer uma das reivindicações 41 a 45, CARACTERIZADA pelo fato de que, após administração a um sujeito humano, a abundância da bactéria é maior do que 1012, 1011, 1010, 109, 108 ou 107 cfu por grama de teor fecal.
47. Composição farmacêutica, CARACTERIZADA pelo fato de que compreende a bactéria de acordo com qualquer uma das reivindicações 1 a 46 e um excipiente farmaceuticamente aceitável.
48. Composição farmacêutica, de acordo com a reivindicação 47, CARACTERIZADA pelo fato de que a composição é formulada como uma cápsula ou um comprimido.
49. Composição farmacêutica, de acordo com a reivindicação 48, CARACTERIZADA pelo fato de que a cápsula é uma cápsula revestida entérica.
50. Composição farmacêutica, de acordo com qualquer uma das reivindicações 47 a 49, CARACTERIZADA pelo fato de que a composição compreende ainda um nutriente privilegiado.
51. Composição farmacêutica, de acordo com a reivindicação 50, CARACTERIZADA pelo fato de que o nutriente privilegiado é um polissacarídeo marinho.
52. Composição farmacêutica, de acordo com a reivindicação 51, CARACTERIZADA pelo fato de que o polissacarídeo marinho é um porfirano.
53. Método de reduzir um nível de oxalato em um sujeito, o método
CARACTERIZADO pelo fato de que compreende administrar a bactéria de acordo com qualquer uma das reivindicações 1 a 46 ou a composição farmacêutica de acordo com qualquer uma das reivindicações 47 a 52 ao sujeito.
54. Método, de acordo com a reivindicação 53, CARACTERIZADO pelo fato de que o sujeito tem um transtorno associado com uma quantidade elevada de oxalato.
55. Método de tratar um transtorno associado com uma quantidade elevada de oxalato em um sujeito, o método CARACTERIZADO pelo fato de que compreende administrar a bactéria de acordo com qualquer uma das reivindicações 1 a 46 ou a composição farmacêutica de acordo com qualquer uma das reivindicações 47 a 52 ao sujeito.
56. Método, de acordo com a reivindicação 54 ou 55, CARACTERIZADO pelo fato de que o transtorno é hiperoxalúria.
57. Método, de acordo com qualquer uma das reivindicações 53 a 56, CARACTERIZADO pelo fato de que compreende ainda administrar um nutriente privilegiado ao sujeito.
58. Método, de acordo com a reivindicação 57, CARACTERIZADO pelo fato de que o nutriente privilegiado é administrado ao sujeito antes, ao mesmo tempo ou depois da bactéria.
59. Método, de acordo com a reivindicação 58, CARACTERIZADO pelo fato de que o nutriente privilegiado é um polissacarídeo marinho.
60. Método, de acordo com a reivindicação 59, CARACTERIZADO pelo fato de que o polissacarídeo marinho é um porfirano.
61. Método, de acordo com qualquer uma das reivindicações 53 a 60, CARACTERIZADO pelo fato de que a bactéria ou composição farmacêutica é administrada ao sujeito a cada 12 horas, 24 horas, dia, 2 dias, 3 dias, 4 dias, 5 dias, 6 dias, 7 dias, semana, 2 semanas, 3 semanas, 4 semanas, mês, 2 meses, 3 meses, 4 meses, 5 meses ou 6 meses.
62. Método, de acordo com qualquer uma das reivindicações 53 a 61, CARACTERIZADO pelo fato de que o tempo entre administrações consecutivas da bactéria ou composição farmacêutica ao sujeito é maior do que 48 horas.
63. Método, de acordo com qualquer uma das reivindicações 53 a 62, CARACTERIZADO pelo fato de que o sujeito é um animal.
64. Método, de acordo com a reivindicação 63, CARACTERIZADO pelo fato de que o sujeito é um ser humano.
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