BR112019026250A2 - CLEANING CLOTH HAVING A RESIDUAL BIOCID PROPERTY AND DISINFECTANT FORMULATION. - Google Patents

Abstract

Disinfectant formulation comprising a poly (2-ethyl-2-oxazoline) and a quaternary ammonium compound or combination of quaternary ammonium compounds is provided. A cleaning cloth having a residual biocidal property is also provided. The cleaning cloth formulation comprises a substrate comprised of a fibrous material, and the formulation present on, or on the substrate. The formulation comprises a poly (2-ethyl-2-oxazoline) and a quaternary ammonium compound or combination of quaternary ammonium compounds, a surfactant, water, and an optional fragrance.

Description

1/38

CLEANING CLOTH HAVING A RESIDUAL BIOCID PROPERTY AND DISINFECTANT FORMULATION CROSS REFERENCE FOR RELATED ORDERS

[001] This application claims priority of the continuation in part of the US application 15 / 625,612 filed on June 16, 2017, which is a continuation in part of the application that claims priority of the continuation in part of the US patent application 15 / 162,068, filed on May 23, 2016, which claims priority for US patent application 14/948962, filed on November 23, 2015, which claims priority for US provisional patent application no. serial number 62 / 084,917, filed on November 26, 2014, and U.S. provisional patent application no. serial number 62 / 127,075, filed on March 2, 2015, and U.S. provisional patent application no. serial number 62 / 166,403, filed on May 26, 2015, with the United States Patent and Trademark Office. The descriptions of which are incorporated herein by reference in their entirety.

FIELD OF THE INVENTION

[002] The present invention relates to the field of disinfectant formulations and, more specifically, to a disinfectant formulation that confers a residual biocidal property.

FUNDAMENTALS OF THE INVENTION

[003] Microbes exist everywhere in the modern world. While some are beneficial to humans and the environment, others can have significant negative consequences for contaminated articles, as well as for people, animals and ecological members who come into contact with them. There are several industries and environments in which such microbes are especially prevalent. Health care

[004] An infection acquired in the hospital (HAI; alternatively, a “hospital infection”) is an infection whose development is favored

2/38 by a hospital or health care environment. Such illnesses are typically fungal or bacterial infections and can affect the victim locally or systemically. Hospital infections can cause severe pneumonia, as well as infections of the urinary tract, bloodstream and other parts of the body.

[005] Hospital infections have serious medical implications for patients and healthcare professionals. In the United States, data suggest that approximately 1.7 million cases of nosocomial infections occur each year, with nearly 100,000 deaths resulting from them. European data and research indicates that Gram-negative bacterial infections alone are responsible for 8,000 to 10,000 deaths each year.

[006] Several aggravating factors contribute to the high rate of HAI. Hospitals, emergency care centers, nursing homes and similar facilities focus their treatment on those with serious illnesses and injuries. As a result, these facilities are home to abnormally highly concentrated populations of patients with weakened immune systems.

[007] A trio of pathogens is commonly found in healthcare facilities and together they are responsible for approximately one third of hospital infections: coagulase-negative Staphilococci (15%), Candida species (11%), and Escherichia coli (10 %).

[008] Worse, it is the pathogens that cause the most robust diseases that are present in such environments. The six species called “ESKAPE pathogens” - Enterococcus faecium, Staphilococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter— are resistant to antibiotics and are implicated in almost half of all hospital infections. Their resistance to one or more biocidal agents makes such infections particularly dangerous.

[009] In particular, the wide nutritional versatility of Pseudomonas allows it to survive in extreme environments, including

3/38 survival on surfaces that are not intensely clean and sterilized. This ubiquity of the pathogen in the hospital environment makes it the main cause of gram-negative hospital infections. Particularly vulnerable are immunocompromised patients (for example, those affected by cystic fibrosis, cancer or burns).

[0010] The most common means of HAI is through the transmission of direct or indirect contact. Direct contact transmission involves a patient in contact with an infected patient or worker. As caregivers move through the health care institution, they come in contact with their many patients. These workers act involuntarily in a manner similar to bees in a garden, "pollinating" rooms and wards as they care for residents.

[0011] Direct contact transmission occurs when the patient comes into contact with a contaminated object or surface. The health care environment features a variety of articles capable of passively transmitting pathogens.

[0012] Hospital infections still cause a serious blow to the volume, quality and cost of health care provided by hospitals and other institutions. In addition to the approximately 100,000 HAI-related deaths that occur annually in the United States, an estimated two million more victims are forced to endure the physical devastation and emotional distress associated with these serious and preventable illnesses.

[0013] The institutions responded by creating policies to impose more stringent cleaning and disinfection requirements on the team and the patient's environment. These programs typically include frequent hand washing and frequent surface disinfection. Despite the implementation of programs to inhibit nosocomial infections, infections still occur at unacceptably high rates.

4/38 Home and household care

[0014] Home environments also face microbes. A major disadvantage associated with disinfectants and sanitizers is that, although they can be effective in killing microbes initially, the surface is easily and quickly recontaminated through contact, airborne microbes and airborne microbes undead before treatment. Although some of the disinfectants continue to offer some control if they are simply left on the surface, this would result in a greasy or sticky residue that would be easily nullified by casual contact with the surface. Thus, there is a desire for a home and household care cleaner that kills microbes quickly when in contact, then acts as a residual disinfectant, but does not yet have this unwanted greasy or sticky effect. Such cleaners can be useful for general household cleaning, bathroom cleaning, and spray protectors.

[0015] The difference between hospital and health care cleaners and household products is the VOC available (volatile organic content). The regulations for most consumer non-aerosol household disinfectants are a maximum of 1% VOC. Food Service

[0016] The food service industry also faces outbreaks of pathogen contamination in the workplace and the spread of disease to consumers. Although food manufacturers adopt vigorous hygiene plans and comply with strict government hygiene regulations, large outbreaks of microbes that cause serious illness among consumers are still occasionally reported. Disinfectants with residual activities should effectively alleviate the problem. Biopellicle

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[0017] A biopellicle is generally defined as a layer of microorganisms that adhere to the surface of a structure, which can be organic or inorganic, that secrete a protective coating that is of biological origin. Biopellets present a major problem for public health due to the increased resistance of organisms associated with biopellets to antimicrobial agents and the potential of organisms transmitted by biopellets to cause infections. Thus, there is a need for a solution for disinfection with respect to biopellicles. Current disinfectants do not have the ability to kill bio-films or to seal or block bio-films on surfaces to prevent cross-contamination events and to prevent growth. There are limited solutions to problems with surface bio-films. For example, there is a body of evidence that surface biopellets pose a problem in the health care environment for the reasons mentioned above.

[0018] In addition, bio-films are a recognized problem of possible contamination in the food industry. Other industries face similar concerns. Thus, there is a need for a solution aimed at potential problem areas of biopellicle that can be used to eradicate biopellicles.

[0019] In summary, there remains a need for a formulation capable of imparting residual biocidal activity to the treated surfaces. It would be additionally advantageous if the formulation were combined with a surface disinfectant, to allow a single cleaning to both disinfect and confer the residual biocidal effect.

[0020] It would be additionally advantageous that the residual biocidal property be associated in a durable way with the treated surface, so that it can continue to provide microbial reduction for an extended period of time after application.

[0021] It would be additionally advantageous if there were formulation (s)

6/38 effective (s) across a wide range of industries and applications.

SUMMARY OF THE INVENTION

[0022] The present invention relates to a disinfectant formulation that confers a residual biocidal property. The disinfectant formulation comprises a polymer binder, wherein the polymer binder is an oxazoline homopolymer or an extended or modified polymer based on an oxazoline homopolymer, and a biocidal compound. The disinfectant formulation additionally comprises a carrier.

[0023] In one aspect of the invention the oxazoline homopolymer has a structure of: wherein R1 is hydrogen, alkyl, alkenyl, alkoxy, alkylamino, alkynyl, allyl, amino, aniline, aryl, benzyl, carboxyl, carboxyalkyl, carboxyalkenyl, cyano, glycosyl, halo, hydroxyl, oxazolinium mesylate, oxazolinium tosylate, oxazolinium triflate, silyl oxazolinium, phenolic, polyalkoxy, quaternary ammonium, thiol, or thioether group; R2 is a hydrogen, alkyl, alkenyl, alkoxy, alkylamino, alkynyl, allyl, amino, aniline, aryl, benzyl, carboxyl, carboxyalkyl, carboxyalkenyl, cyan, glycosyl, halo, hydroxyl, oxazolinium mesylate, oxazoline oxide, triflate oxide , silyl oxazoline, phenolic, polyalkoxy, quaternary ammonium, thiol, or thioether group or a macrocyclic structure; R3 is a hydrogen, alkyl, alkenyl, alkoxy, aryl, benzyl, hydroxyalkyl, or perfluoroalkyl group; and n is in a range of 1 to 1,000,000.

[0024] In another aspect of the invention other characteristics of the disinfectant formulation (s) are provided.

[0025] In yet another aspect of the invention, an article having the disinfectant formulation (s) of the present invention is provided, as well as

7/38 methods of producing, using and applying the disinfectant formulation (s).

[0026] Additional areas of applicability of the present invention will become apparent from the detailed description provided in the following parts. It should be understood that the detailed description and specific examples, while indicating the preferred embodiments of the invention, are intended for illustration purposes only and are not intended to limit the scope of the invention.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0027] The following description of the modalities of the present invention is only exemplary in nature and is not intended to limit the invention, its application or uses. The present invention has a wide application potential and utility, which is considered to be adaptable across a wide range of industries. The following description is provided here only as an example in order to provide a description that permits the invention, but does not limit the scope or substance of the invention.

[0028] As used here, the terms "microbe" or "microbial" should be interpreted as referring to any of the microscopic organisms studied by microbiologists or found in the environment of use of a treated article. Such organisms include, but are not limited to, bacteria and fungi, as well as other single-celled organisms such as mold, mildew and algae. Viral particles and other infectious agents are also included in the term microbe.

[0029] "Antimicrobial" must be understood additionally as having microbicidal and microbistatic properties. That is, the term comprises killing of microbes, leading to a reduction in the number of microbes, as well as a retarding effect on microbial growth, in which the numbers can remain more or less constant (but still allowing for a slight increase / decrease ).

[0030] To facilitate discussion, this description uses the term

8/38 antimicrobial to denote a broad spectrum activity (for example, against bacteria and fungi). When talking about efficacy against a particular microorganism or a taxonomic classification, the most focused term will be used (for example, antifungal to denote effectiveness against the growth of particular fungi).

[0031] Using the example above, it should be understood that the efficacy against fungi does not in any way exclude the possibility that the same antimicrobial composition can demonstrate efficacy against another class of microbes.

[0032] For example, the discussion of the strong bacterial efficacy demonstrated by a described modality should not be read to exclude that modality from also demonstrating antifungal activity. This method of presentation should not be construed as limiting the scope of the invention in any way. Disinfectant Formulation

[0033] The present invention is directed to a disinfectant formulation. In one aspect of the invention, the disinfectant formulation is in liquid form. The composition of the disinfectant formulation comprises a biocidal compound and a polymer binder. The composition can additionally comprise a solvent (such as water or a low molecular weight alcohol), a surfactant, a colorant, a fragrance, among other components.

[0034] A liquid composition is a formula having residual biocidal properties and surface disinfection. The formulation can be applied to a surface by spraying, rolling, steaming, scrubbing or other means. The formulation acts as a surface disinfectant, killing infectious microbes present on the surface.

[0035] Once dry, the liquid formulation leaves a residual protective film on the surface. The residual film has a property

9/38 biocide, allowing it to maintain surface protection against microbial contamination for a prolonged period of time after application.

[0036] In a preferred embodiment, the surface disinfectant formulation provides a film with the ability to quickly kill bacteria and other germs for at least 24 hours after depositing the film on the treated surface. In one aspect of the invention, rapid death generally refers to a period of time from about 30 seconds to about 5 minutes. The film will remain on the surface and is durable to multiple touches and surface wear.

[0037] In another embodiment of the present invention, the disinfectant formulation is a liquid composition comprising a polymer binder, a biocidal compound, a carrier such as a solvent, and other additional components such as fragrances. Bio-Film Sealant

[0038] In one embodiment of the present invention, the disinfectant formulation is a bio-film sealant. The bio-film sealant, once applied to a surface, forms a polymer film. The polymer film is applied wet and dry as a film layer to block and prevent subsequent growth of microorganisms on the film. The bio-film sealant, although preferably in the form of a liquid, can also take other shapes such as a gel, or other shape. Once the bio-film sealant is in place, microbes in the bio-film have limited access to oxygen and exogenous nutrients. The bio-film sealant has the potential to seal bacteria, but it also provides release of antimicrobials in the film to kill microorganisms in the film.

[0039] In one embodiment of the present invention, the bio-film sealant comprises a polymer binder and a biocidal compound. The biocidal compound may include, but is not limited to, any compound

10/38 biocide defined here.

[0040] In one embodiment of the present invention, the bio-film sealant comprises an oxazoline homopolymer as the polymer binder. The oxazoline homopolymer can have any of the structures as defined here.

[0041] In one embodiment of the present invention, the bio-film sealer comprises a polymer binder, a biocidal compound, and an enzyme (s). The enzyme is to assist the degradation of the bio-film and provides enhanced antimicrobial penetration by the biocidal agent and finally removal of the bio-film. Examples of enzymes include, but are not limited to, specific proteinase, DNase, RNase, and carbohydrate enzymes that can degrade the extracellular matrix associated with the biopellicle.

[0042] In one embodiment of the present invention, the bio-film sealant comprises a polymer binder and an antibody as a biological material for slow release in the film. The antibody's functionality will bind to the microbe of the bio-film to prevent cross-contamination.

[0043] In one embodiment of the present invention, the bio-film sealer comprises a polymer binder, and a bacteriophage or a mixture of bacteriophages as a biological material for slow release in the film. The bacteriophage acts as an antimicrobial agent directed to kill organisms associated with the bio-film.

[0044] In one embodiment of the present invention, the bio-film sealant comprises a polymer binder, and a mixture of bacteriophages, antimicrobial agents, enzymes and antibodies as a biological material for slow release in the film. Polymer Binder

[0045] In one aspect of the invention, the polymer binder is a

11/38 oxazoline homopolymer.

[0046] As another feature of the invention, the oxazoline homopolymer has the following structure: wherein R1 and R2 are terminal groups determined by polymerization techniques used to synthesize oxazoline homopolymer. R1 and R2 are independently selected and include, but are not limited to, hydrogen, alkyl, alkenyl, alkoxy, alkylamino, alkynyl, allyl, amino, aniline, aryl, benzyl, carboxyl, carboxyalkyl, carboxyalkenyl, cyan, glycosyl, halo, hydroxyl , oxazolinium mesylate, oxazolinium tosylate, oxazolinium triflate, silyl oxazoline, phenolic, polyalkoxy, quaternary ammonium, thiol, or thioether groups. Alternatively, R2 may include a macrocyclic structure formed during synthesis, as a consequence of an intramolecular attack.

[0047] For example, R1 is a methyl group and R2 is oxazolinium tosylate if methyl tosylate is used as the initiator in oxazoline initiated cationic polymerization.

[0048] R3 is a terminal group determined by the type of oxazoline used in the preparation of the polymer binder of this invention. R3 includes, but is not limited to, hydrogen, alkyl, alkenyl, alkoxy, aryl, benzyl, hydroxyalkyl, or perfluoroalkyl. For example, R3 is an ethyl group if ethyloxazoline is the monomer used to prepare the polymer binder for the present invention.

[0049] n is the degree of polymerization of oxazoline in the homopolymer. n is in a range of 1 to 1,000,000. Preferably, n is in the range of 500 to 250,000; most preferably, n is in a range of 2500 to

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100,000.

[0050] Similar to the oxazoline homopolymer, extended or modified polymers with some variations based on the oxazoline homopolymer are also suitable for the present invention. The techniques and options for carrying out chemical variations or modifications or molecular structure on oxazoline are familiar to those skilled in the art. A class of extended or modified polymers based on the oxazoline homopolymer can be represented with the following molecular structure: where R1 and R3 have the same definition as that given in the oxazoline homopolymer above.

[0051] B is an additional monomer repeat unit attached to oxazoline in a copolymer. The types of arrangement of the repeating units between B and oxazoline in the copolymer may include, but are not limited to, block, alternating, periodic, or combinations thereof. There are no limitations on the types of B that can be used to copolymerize with or modify the oxazoline of the present invention.

[0052] n is the degree of polymerization for an oxazoline repeat unit; n in the copolymer is in a range of 1 to 1,000,000 and the degree of polymerization for B repeat unit in copolymer m is in a range of 0 to 500,000 at the same time. Preferably, n is in the range of 500 to 250,000 and m is in the range of 20 to 10,000; and most preferably, n is in the range of 2500 to 100,000 and m is in the range of 50 to 5,000. In addition to binding B to ethyloxazoline via copolymerization, B could also be attached to oxazoline as a terminal group in a cationic polymerization using B as an initiator

13/38 cationic if B itself is already a quaternary ammonium compound.

[0053] Not planning to be inclusive, B can be, for example, ethyleneimine with the following molecular structure: where the end groups R1 and R2 have the same definition as defined for the oxazoline homopolymer.

[0054] R3 includes, but is not limited to, hydrogen, alkyl, alkenyl, alkoxy, aryl, benzyl, hydroxyalkyl, or perfluoroalkyl.

[0055] R4 includes, but is not limited to, hydrogen, alkyl, alkenyl, alkoxy, aryl, benzyl, hydroxyalkyl, or perfluoroalkyl.

[0056] m is in a range of 0 to 500,000; preferably, in a range of 20 to 10,000; and most preferably, in a range of 50 to 5,000.

[0057] n is in a range of 1 to 1,000,000; preferably 500 to 250, .00; most preferably, in a range of 2500 to 100,000.

[0058] The synthesis of oxazoline and ethyleneimine copolymer can be carried out in two stages, for example. In a first step, a cationic ring opening polymerization technique can be used to produce polyoxazoline homopolymer. In a second step, the polyoxazoline produced in the first step can be hydrolyzed to convert part of a polyoxazoline repeating unit into polyethyleneimine. Alternatively, the oxazoline-ethylenimine copolymer can be produced with the appropriate appropriate monomers, an oxazoline and an aziridine. The result would be a cationic polymer having the above structure.

[0059] The degree of para per repeat unit of oxazoline n in the copolymer is in a range of 1 to 1,000,000 and the degree of polymerization for repeat unit of ethyleneimine in copolymer m is in a range

14/38 from 0 to 500,000 at the same time. Preferably, n is in a range of 500 to 250,000 and m is in a range of 20 to 10,000, and most preferably n is in a range of 2500 to 100,000 and m is in a range of 50 to 5,000.

[0060] Alternatively, the nitrogen in the ethyleneimine repeating unit could be additionally quaternized to generate the following cationic copolymer:

[0061] Any quaternization technique that is familiar to those skilled in the art can be used to quaternize the polymer of this example. R1, R2, R3 and R4 have the same meaning as designated in the above oxazoline-ethyleneimine copolymer. R5 includes, but is not limited to, a hydrogen, methyl, ethyl, propyl, or other types of alkyl group. The corresponding X anion is a halogen, sulfonate, sulfate, phosphonate, phosphate, carbonate / bicarbonate, hydroxy, or carboxylate.

[0062] The ranges for n and m are also the same as those described in the oxazoline-ethyleneimine copolymer.

[0063] Another example of B that can be used for the present invention is polydialyldimethylammonium chloride. Polyethyloxazoline modified with polydialyldimethylammonium chloride has the following structure: wherein R1 and R4 have the same meaning as described in the previous example for quaternized oxazoline-ethyleneimine copolymer.

[0064] R2 and R3, independently, include, but are not limited to, lower alkyl groups such as C1 to C6. The corresponding X anion

15/38 is a halogen, sulfonate, sulfate, phosphonate, phosphate, carbonate / bicarbonate, hydroxy, or carboxylate.

[0065] n and m are defined and numbered in the same way as in the previous examples.

[0066] B can be other olefins including, but not limited to, diallyldimethylammonium chloride, styrene, methoxystyrene, and methoxyethene. Ethoxoxazoline can also be copolymerized with heterocyclic monomers such as oxirane, 1,3-dioxepan tiethane, oxetan-2-one, and tetrahydrofuran to enhance the performance of the polymer for the present invention. The binder used in this invention can also employ oxazoline groups dangling from a polymer backbone, such as an acrylic or styrene-based polymer, or a copolymer containing acrylic or styrene.

[0067] Examples of commercially available polyethyloxazolines include, but are not limited to, Aquazol 500 from Polymer Chemistry Innovations, Inc.

[0068] The amount of polymer binder that can be used in the liquid formulation may vary slightly, depending on the desired length of the residual activity of the composition and the nature of all other components in the composition. Preferably, the amount of polymer binder in the liquid formulation is in the range of 0.1% to 20% based on the weight of the liquid formulation. In a liquid formulation for healthcare applications, the amount of polymer binder in the liquid formulation is most preferably in the range of 0.5% to 10%, and most preferably in the range of 0.8% to 5% . In liquid formulations for general purpose and bathroom cleaners, the amount of polymer binder in the liquid formulation is most preferably in the range of 0.1% to 10%, and most preferably in the range of 0.1% to 5% .

[0069] The polymer binder is preferably soluble in water and

16/38 can be quickly removed if any build-up is observed. Present in small quantities, it can still provide a durable bond between the biocidal compound and the treated surface to facilitate residual effectiveness. Biocidal Compound

[0070] The biocidal compound may be a quaternary ammonium compound (QAC) with the following molecular structure: wherein R1, R2, R3, and R4 are independently selected and include, but are not limited to, alkyl, alkoxy, or aryl , either with or without heteroatoms, or saturated or unsaturated. Some or all of the functional groups can be the same.

[0071] The corresponding X anion includes, but is not limited to, a halogen, sulfonate, sulfate, phosphonate, phosphate, carbonate / bicarbonate, hydroxy, or carboxylate.

[0072] QACs include, but are not limited to, n-alkyl dimethyl benzyl ammonium chloride, n-octyl dimethyl ammonium chloride, dodecyl dimethyl ammonium chloride, n-alkyl dimethyl benzyl ammonium saccharate, and 3- ( trimetoxisily1) propyl dimethyloctadecyl ammonium.

[0073] Combinations of monomeric QACs are preferred for use in the present invention. A specific example of a QAC combination is N-alkyl dimethyl benzyl ammonium chloride (40%); N-octyl decyl dimethyl ammonium chloride (30%); di-n-decyl dimethyl ammonium chloride (15%); and di-n-dioctyl dimethyl ammonium chloride (15%). The percentage is the percentage by weight of individual QACs based on the total weight of QACs composition

17/38 mixed.

[0074] The polymeric version of the QACs with the following structures can also be used for the invention.

or where R1, R2, R5, and R6, independently, include, but are not limited to, hydrogen, methyl, ethyl, propyl or other longer carbon alkyl groups.

[0075] R3 and R4 are independently selected and include, but are not limited to, methylene, ethylene, propylene or other longer alkylene linking groups.

[0076] n is the degree of polymerization; n is an integer in a range of 2 to 10,000.

[0077] Examples of cationic polymers with the above structure include, but are not limited to, polyamines derived from dimethylamine and epichlorohydrin such as Superfloc C-572 commercially available from Kemira Chemicals.

[0078] Yet another polymeric QAC suitable for the invention is diallyldimethylammonium polychloride or polyDADMAC.

[0079] Yet another class of QACs useful for the present invention are chemical compounds with a portion of biguanide in the molecule. Examples

18/38 of this class of cationic antimicrobials include, but are not limited to, PHMB and chlorhexidine.

[0080] Examples of commercially available quaternary ammonium compounds include, but are not limited to, Bardac 205M and 208M from Lonza, and BTC885 from Stepan Company.

[0081] The biocidal compound may be a weak acid, which has been shown to be particularly effective in bathroom cleaners. In these types of products, citric, sulfamic acids (also known as amidosulfonic acid, amidosulfuric acid, aminosulfonic acid, and sulfamidic acid), glycolic, lactic, lauric and capric acid are useful as both, an effective biocide and a cleaning agent for foam. soap and hard water deposits.

[0082] Other compounds that may be useful are quaternary silane salts such as 3 (trihydroxysilyl) propyldimethyloctadecyl ammonium chloride. This can have the added benefit of reacting with the surface being treated for an intensification of residual properties.

[0083] Additional biocidal compounds suitable for use in the present liquid formulation cover a range of antimicrobials, biocides, sanitizers, and disinfectants. A water-soluble or dispersible biocidal compound is preferred, although alcohol-soluble biocides may be employed alternatively.

[0084] A non-exhaustive list of biocidal compounds suitable for use in the present formulation includes triclosan, zinc pyrithione, metal salts and oxides, phenols, botanicals, halogens, peroxides, heterocyclic antimicrobials, aldehydes, and alcohols.

[0085] The concentration of biocidal compound in the formulation can be in a range of 0.05% to 20% based on the weight of the liquid composition. For a liquid formulation for a health care application, preferably in a range of 0.1% to 20%, and more preferably in a range of 0.5% to 3%. For an all-purpose liquid formulation and

19/38 bathroom cleaners, preferably in a range of 0.05% to 10%. For a formulation for a protector, preferably in a range of 0.05% to 2%. Carrier

[0086] The carrier or medium for the liquid formulation of this invention can be any solvent that is volatile and allows easy evaporation in the ambient condition. Examples of liquid carriers include, but are not limited to, water and low molecular weight alcohols such as C1 to C8 alkanols. Specific examples include, but are not limited to, ethanol, isopropyl alcohol, butanol, pentanol, and combinations thereof.

[0087] Another class of solvents for use in the invention includes alkylene glycol ether. Examples include, but are not limited to, ethylene glycol monopropyl ether, ethylene glycol monobutyl ether, ethylene glycol monohexyl ether, ethylene glycol monohexyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol monohexyl ether , triethylene glycol monomethyl ether, triethylene glycol monoethyl ether, triethylene glycol monobutyl ether, propylene glycol methyl ether, propylene glycol methyl ether, propylene glycol n-butyl ether, dipropylene glycol n-butyl ether, dipropylene glycol methyl ether, dipropylene acetate methyl ether glycol, propylene glycol n-propyl ether, dipropylene glycol n-propyl ether, and tripropylene glycol methyl ether.

[0088] Another class of solvents for use in the invention is based on terpenes and their derivatives such as terpene alcohols, terpene esters, terpene ethers, or terpene aldehydes. Examples of solvents include, but are not limited to, pine oil, lemon oil, limonene, pinene, cimene, mircene, fenchone, borneol, nopol, cineole, ionone and the like.

[0089] A preferred carrier in a liquid formulation for a home care cleaning application is water.

[0090] If the method of applying the liquid formulation of the present invention is pressurized aerosol, a propellant may be required in

20/38 composition. A variety of propellants or mixtures can be used for the present invention and should be familiar to those skilled in the art. C1 to C10 hydrocarbons or halogenated hydrocarbons are typical propellants in aerosol compositions known in the industry. Examples of such propellants include, but are not limited to, pentane, butane, propane, and methane. Other types of propellants that can be used for the present invention also include compressed air, nitrogen or carbon dioxide. Alternatively, a bag in the valve package can be used to aerosolize the product without directly adding a propellant to the composition.

[0091] Both a single solvent and a mixture of the above solvents can be used for the present invention. The types of solvents used for the present invention may depend on the intended uses of the residual disinfectant composition. For example, if the composition of the present invention is intended for use in home care, cleaning contaminated surfaces free of all types of stain or dirt may be of primary interest. A carrier or liquid medium that helps and intensifies the removal of dirt can be the formulation of the invention. For example, the residual disinfectant formulation or composition of the present invention may wish to include alkyl or multiple alkyl glycols ethers for better cleaning performance in the home care version of the formulation of the present invention. On the other hand, if the primary purpose of the residual disinfectant composition is to be used in a health care facility where the greatest concern is hospital acquired infection, then quick drying of the liquid composition of the present invention may be more desirable than cleaning from stains or dirt on the surface. Low molecular weight alcohols should be considered to help the liquid formulation of the present invention to dry quickly after application. In addition, a low molecular weight alcohol in the liquid formulation will strengthen the sanitizing activity of the liquid composition.

21/38

[0092] For health care use of residual disinfectant, a mixture of water and low molecular weight alcohol is preferred. It is preferred that the amount of alcohol present in the liquid formulation is at such a level that the liquid formulation is capable of forming a zeotropic mixture between alcohol and water. A minimum amount of alcohol, if present, in the liquid composition is 10%. Preferably, for health care use of the residual disinfectant, the alcohol concentration is 30%, and most preferably the alcohol concentration is at least 50% based on the weight of the liquid formulation for health care use of the composition of the invention. . Surfactant

[0093] A surfactant or wetting agent can be used. The surfactant assists the spreading and uniform coating of the liquid formulation on the surface being treated. The surfactant additionally contributes to the formation of a zeotropic mixture between alcohol and water, thus facilitating the rapid and uniform drying of the liquid formulation once applied on the surface. A surfactant also plays an important role in the residual liquid disinfectant formulation of the present invention for domestic use if dirt cleaning performance is the main feature that the product is designed to have.

[0094] Surfactants suitable for the present liquid formulation include, but are not limited to, those that are non-ionic, anionic, or amphoteric in nature. Examples of commercially available wetting agents include, but are not limited to, Ecosurf SA-4 or Tergitol TMN-3 from Dow Chemical, and Q2-5211 from Dow Corning.

[0095] An amine oxide surfactant is preferred especially when QAC is used as the biocidal compound in the formulation.

[0096] In the category of non-ionic surfactants, ethoxylated alcohols with different amounts of ethylene oxides or HLB values can be used. Examples of ethoxylated alcohols include, but are not limited to,

22/38 Triton X-100 (Dow Chemical, Midland MI), Dow Chemical's Ecosurf EH nonionic surfactant series, Dow Chemical's Tergitol nonionic surfactant series, Huntsman Corp.'s sulfonic surfactant series, the surfactant series Shell Neodol, the Ethox surfactant series from Ethox Chemicals and the Tomadol surfactant series from Air Products and Chemicals, Inc.

[0097] Another class of non-ionic surfactants include alkyl polyglucosides. Examples include BASF's Glucopon series and Huntsman's Ecoteric series.

[0098] An alternative class of surfactants that is preferred for the liquid formulation are silane-based surfactants. Examples include, but are not limited to, organofunctional or silane reactive silicone polyether wetting agents, and fluorochemical based wetting agents.

[0099] The surfactant content in the liquid formulation is in a range of 0% to 10%, preferably in a range of 0.01% to 5%.

[00100] Depending on the intended uses, a liquid formulation of the present invention for use in home care may require appropriate pH conditions. For example, if the liquid product is used in the kitchen area, a product with a high pH may be desired in order to effectively remove greasy dirt commonly found in the area. If the product is used in the bathroom area, soap foam and hard water deposits may be the main concern. In such a case, a product with a low pH may be more appropriate for this purpose. There is no limitation on the types of pH adjusting agents that can be added to the liquid composition of the present invention. Examples of pH adjusting agents that can be used include, but are not limited to, triethanolamine, diethanolamine, monoethanolamine, sodium hydroxide, sodium carbonate, potassium hydroxide, potassium carbonate, calcium carbonate, acid

23/38 citric, acetic acid, hydrochloric acid, sulfamic acid, sulfuric acid and the like.

[00101] In addition to the components mentioned above, additional functional components can be included in the liquid composition of the present invention. Additional components include, but are not limited to, chelators, compatibilizers, coupling agents, corrosion inhibitors, rheology modifiers, fragrances, colorants, preservatives, UV stabilizers, optical brighteners, and active ingredient indicators.

[00102] In one embodiment of the present invention, the liquid solution comprises a polymer binder, a quaternary ammonium compound, a silicone-based surfactant, and ethanol. The liquid formulation can be made or mixed by any conventional method known to those skilled in the art. There are no preferred addition procedures for the formulation of the present invention as long as the formulation is fundamentally homogeneous, compatible and stable. For example, if the polymer binder is a solid, it may be preferable to first dissolve or disperse the polymer in a carrier such as water or alcohol to stock the liquid polymer binder dispersion. The stock of the liquid polymer binder dispersion can be readily added to the formulation of the present invention during the mixing procedure. Liquid Formulation Application

[00103] The liquid formulation can be applied by a variety of means. If sprayed, the liquid formulation can advantageously be supplied in a conventional bottle with a sprayer. The sprayer can be a trigger sprayer. As an option for a trigger spray, an aerosol can also be used to distribute the liquid formulation to surfaces. Additional means of application include, but are not limited to, steam, rolling, brushing, scrubbing,

24/38 and using a cleaning cloth for a variety of application devices. It is within the scope of the present invention that cleaning products may also be made comprising or pretreated with the disinfectant formulation (s) of the present invention, for example, for sale or use alone.

[00104] In one embodiment of the invention, a cleaning cloth is provided having up to 24 hours of residual sanitizing activity. The cleaning cloth is able to protect a hard growth surface and protect against bacteria without leaving a significant residue or build-up.

[00105] In one embodiment of the invention, the cleaning cloth is comprised of a substrate and a formulation applied to the substrate or present on the substrate of the cleaning cloth.

[00106] The cleaning cloth formulation provides a solid transparent film soluble in water enough to be easily removed with additional cleaning, but durable enough to remain on the surface even after being touched.

[00107] The substrate is any fibrous material capable of absorbing and / or adsorbing water, preferably at least three times its weight in water. The substrate comprises a synthetic material, a natural material, or a combination thereof. Examples of synthetic materials include, but are not limited to, polypropylene, polystyrene, polyester, nylon, rayon, acrylic, spandex, and a combination thereof. Examples of natural materials include, but are not limited to, cotton, linen, silk, wool, hemp, linen, and a combination thereof.

[00108] The substrate can be woven or non-woven. A non-woven substrate can be made by any of several manufacturing methods. The resulting non-woven substrate can be, but is not limited to, melt blown, co-formed, spunbonded, airway / airflow, hydroentangled, spunlaced, bonded, carded, laminated, or a combination thereof. For example, a substrate can comprise a polymeric material blown in

25/38 merger.

[00109] In one embodiment of the invention, the cleaning cloth formulation comprises a biocidal compound, a polymer binder and a wetting / surfactant.

[00110] For a substrate comprising a natural material, the cleaning cloth formulation may comprise a cationic-containing compound that is designed to compete with the quaternary ammonium compound (s) for the locations of cationic species on the substrate. Salts with high ionic strengths can also be used. Examples of salts for use in formulating cleaning cloths include, but are not limited to, potassium citrate, sodium citrate, magnesium sulfate, sodium chloride, ammonium chloride, potassium chloride, and a combination thereof.

[00111] The formulation of cleaning cloths provides initial disinfection so, once dry it leaves a residual protective film on the surface. The film has biocidal properties with the ability to kill bacteria, for example, within a contact time of 5 minutes for up to 24 hours after initial application.

[00112] In a preferred embodiment, the cleaning cloth formulation comprises a poly (2-ethyl-2-oxazoline), a quaternary ammonium compound or a combination of quaternary ammonium compounds, an alcohol, water ethoxylate, and an optional fragrance.

[00113] Quaternary ammonium compounds (QAC) are especially suitable for this cleaning cloth application as they provide protection against a wide range of microorganisms. Examples of such compounds are available commercially from Stepan Chemical under the trade name BTC885. BTC885 contains 50% of a mixture of quaternary ammonium compounds. A similar material is also commercially available from Lonza under the trade name Bardac 205M.

[00114] Preferably, the quaternary ammonium compound and the

26/38 binders are in a ratio of quaternary ammonium compound to binder from 1: 4 to 1: 1.

[00115] Preferably, the cleaning cloth formulation comprises a poly (2-ethyl-2-oxazoline) in an amount of 1% by weight to 5% by weight, where the percentage by weight is based on the total weight of the formulation of cleaning cloths.

[00116] Preferably, the cleaning cloth formulation comprises a quaternary ammonium compound in an amount of at least 0.2 weight percent, preferably 0.2 weight percent to 2 weight percent, where the percentage in weight is based on the total weight of the cleaning cloth formulation.

[00117] Preferably, the formulation of cleaning cloths comprises a non-ionic surfactant.

[00118] In one embodiment of the invention, the non-ionic surfactant is an alcohol ethoxylate. Preferably, the cleaning cloth formulation comprises alcohol ethoxylate in an amount of 0.05% by weight to 0.5% by weight, where the weight percentage is based on the total weight of the cleaning cloth formulation. A preferred alcohol ethoxylate is a linear alcohol ethoxylate with at least 9 moles of ethylene oxide, more preferably with at least 12 moles of ethylene oxide. It is unexpected that an alcohol ethoxylate, as in this group, when used in the formulation of the present invention surprisingly and favorably contributes to the aesthetics of the resulting film by drying on a hard transparent film (as opposed to a sticky, cloudy film) still remains soluble in enough water to allow the release of the quaternary ammonium compound (s) for antimicrobial effectiveness.

[00119] A weak acid, including, but not limited to, citric acid, lactic acid, sulfamic acid, glycolic acid, or a combination thereof, can be added to the formulation to increase effectiveness against

27/38 gram-negative bacteria and assist in removing dirt from hard water stains or soap foam.

[00120] Ethylenediaminetetraacetic acid, disodium salt or EDTA disodium salt can be added in the formulation to increase the antimicrobial effectiveness in the presence of hard water.

[00121] The formulation of cleaning cloths optionally comprises a fragrance. If a fragrance is present, the cleaning cloth formulation preferably comprises a fragrance in an amount from 0% by weight to 0.5% by weight, where the weight percentage is based on the total weight of the cleaning cloth formulation .

[00122] Water is a suitable carrier for aggregates and active materials. Solvents such as ethanol or isopropanol are optionally added to decrease drying time.

[00123] The cleaning cloth formulation of the present invention is effective for the above purposes without requiring other components to be present in the formulation, although other components may be present. For example, the cleaning cloth formulation of the present invention is effective for its residual sanitizing activity without hydrogen peroxide as a component of the formulation.

[00124] To disinfect a contaminated surface, spray the liquid formulation until the area is completely covered. The wet formulation can subsequently be dry cleaned with a dry cloth or paper towel.

[00125] The invention also relates to an article treated with a disinfectant formulation according to aspects of the invention. Examples

[00126] The following examples illustrate liquid formulations made according to aspects of the present invention. The test results in these formulations demonstrate the desired sanitizing performance or

28/38 residual disinfection once applied on surfaces and dried. The cleaning performance is also tested in formulations that provide not only the benefit of residual disinfection, but also cleaning characteristics.

[00127] The formulations were tested for residual efficacy using the EPA 01-1A protocol. Briefly, bacteria were added to a glass slide and allowed to dry on the surface. The formulation was then sprayed onto the surface and dried to form a clear film. Once the film was formed, the glass slide was exposed to alternating wetting and drying cycles using the Gardner wear tester described in the protocol. Between each cycle the slide was reinoculated with bacteria. After the appropriate number of wear and reinoculations (48 passes and 11 reinoculations for health care formulation and 24 passes, 5 reinoculations for home care formulation) the slide was exposed to bacteria for the indicated time period (ie 5 minutes ) followed by recovery in an appropriate neutralizing solution.

[00128] In addition to residual effectiveness, the initial effectiveness of the composition of the present invention was also tested according to ASTM E 1153.

[00129] A modified ASTM D4488 was used to evaluate the cleaning performance of hard surfaces for the home care composition of the present invention. A dirt with the following composition was used for the evaluation. Table 1 Components Percentage by weight of each component (%) Pure vegetable oil 75 Carpet dirt TM-122 AATCC 25 * Carpet dirt TM-122 AATCC was obtained from Textile Innovators.

[00130] In the process of making a dirty ceramic tile for the cleaning test, around 2 grams of the liquid dirt was placed on an aluminum sheet. A roller was used to roll and spread the dirt over the leaf and let the roller catch as much dirt as possible. The dirty

29/38 on the roller was transferred uniformly to the glazed surface of a ceramic tile by rolling the dirty roller over the ceramic surface. The dirty ceramic tile was then baked in an oven set at 180C for 45 minutes. The baked tile was conditioned at room temperature for 24 hours before being used for the cleaning test.

[00131] A Gardner wear tester was used in the cleaning test. Cleaning pads around 1 cm wide were attached to the abrasion boat for wear. Around 4 grams of test formulation were placed in a weighing boat. The cleaning pad was immersed inside the weighing boat to pick up the test formulation.

[00132] The cleaning process was started immediately after the pad was moistened with the cleaning formulation. Seven wear cycles (back and forth) were used in the test. Examples of residual disinfectant for healthcare

[00133] The following formulation in the example uses alcohol as the main carrier in order to provide quick drying properties for liquid formulations. Table 2 Components HE1 HE2 HE3 (% by weight) (% by weight) (% by weight) Water remainder remainder Ethanol 70 70 0 2-Propanol 0 0 70 Polyethyloxazoline 2 2 2 Quaternary ammonium compound 0.8 1.2 1 , 2 Wetting Agent / Surfactant 0.1 0.1 0.1

[00134] The residual effectiveness test was conducted using the EP01-1A protocol and the results are listed in the following table. Table 3 FormulationEP01-1A (average bacterial log reduction) HE1 3.53 HE2 5.50 HE3 4.50

[00135] These formulations show an excellent result of effectiveness

30/38 residual based on the EP01-1A test.

[00136] The ASTM E 1153 test protocol was also followed to assess the initial biocidal property of HE2. The test results are shown in the following table. Table 4 Initial Efficacy Time Method Bacterials 3 log reduction Complete death (<10 CFU / PFU) Klebsiella pneumoniae 30 seconds 1 minute ASTM E 1153 Pseudonomas aerugniosa 30 seconds 30 seconds ASTM E 1153 Staphylococcus aureus 30 seconds 30 seconds ASTM E 1153 MRSA 30 seconds 30 seconds ASTM E 1153 VRE 30 seconds 30 seconds ASTM E 1153 Enterobacter aerogenes 30 seconds 30 seconds ASTM E 1153 Enterococcus faecallis 30 seconds 1 minute ASTM E 1153 Fungi Aspergillus niger 1 minute 5 minutes ASTM E 1153 Tricophyton 1 minute 5 minutes ASTM And mentagrophytes 1153 Viral HINI (envelope) 30 seconds 30 seconds ASTM 1053 MS2 (non-enveloped) 30 seconds 5 minutes ASTM 1053 Residual efficacy Log reduction time period Exposure method Pseudonomas aerugniosa 5 minutes> 3 EPA 01-1A Enterobacter aerogenes 5 minutes> 3 EPA 01-1A Staphylococcus aureus 5 minutes> 3 EPA 01-1A

[00137] These data clearly demonstrate that the sample surfaces treated with the exemplary liquid formulation described here have demonstrable biocidal activity over the indicated period of time. Examples of home care residual disinfectant cleaner

[00138] These compositions are formulated using water as the vehicle. They are designed for use in home care where VOC regulations prohibit the use of high levels of organic solvents such as alcohols. Table 5

31/38 Components H1 H1 H1 H1 H1 (% by weight) (% by weight) (% by weight) (% by weight) (% by weight) Water rest rest rest rest EDTA tetrasodium ED 0 0 0 0 0 0.4 Polyethyloxazoline1 1 1 1 0.5 0.5 Ethoxylated alcohol # 1 0.33 0 0 0 0 Ethoxylated alcohol # 2 0 0 0.2 0.2 0.2 Quaternary ammonium compound 0.4 0.4 0.4 0, 4 0.4 Ethanolamine 0.2 0.2 0.2 0.2 0.2 Wetting agent 0.1 0.1 0.1 0.1 0.1 0.1

[00139] The residual effectiveness of these formulations was evaluated using the EP01-1A protocol and the results are listed in the following table. Table 6 FormulationEP01-1A average log bacterial reduction H1 3.53 H2 5.50 H3 5.50 H4 4.90 H15 3.80

[00140] Enterobacter aerogenes were the bacteria for the H1 test and Staphilococcus aureus was the bacteria used in the test for the rest of the formulations.

[00141] The test results showed that all H1 to H5 provided residual efficacy for the treated surfaces. Cleaning performance was also assessed using the modified ASTM D4488 test method.

[00142] The test results also showed clearly visually that the formulation of the present invention provided not only residual efficacy against bacteria, but also good cleaning performance on dirty surfaces.

[00143] Additional formulations defined in the tables below have been tested for home care and home cleaning applications. To solubilize the fragrance, a premix is prepared containing the fragrance, composed of quaternary ammonium, surfactant and glycol ether if present. Table 7 - Protective formulations for light services Component P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12 P13 P14 P15 (% (% (% (% (% (% (% (% (% (% (% (% (% (% (% (% em em em em em em em em em em em em em weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) Polyethyl - 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 0.50 1.0 0.50 1.0 0.50 oxazoline

32/38 Compound of 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.20 0.20 0.20 0.10 0.10 quaternary ammonium

Fragrance 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Agent of 0, 30 0.10 0.10 0.10 0.10 0.10 0.10 wetting 0.30 0.30 0.30 0.30 0.30 amine Surfactant 0.30 ethoxylated cationic Quat dicoco 0.30 Alcohol 0.30 ethoxylated Tri- 0.50 0.50 0.50 0.50 0.50 0.50 0.50 ethanolamine NaEDTA 0.10 0.10 metasilicate sodium pentahydrate 0.10 sodium carbonate Water * BBBBBBBBBBBBBBB

Component P16 P17 P18 P19 P20 P21 P22 P23 P24 P25 P26 P27 P28 P29 (% (% (% (% (% (% (% (% (% (% (% (% (% (% (% in in in in in in in em em em em em by weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) Polyethyl- 1.0 0.50 1.0 0.50 1 , 0 0.50 1.0 0.50 1.0 1.0 1.0 0.50 0.50 0.50 oxazoline Compound of 0.20 0.20 0.10 0.10 0.20 0.20 0.10 0.10 0.20 0.20 0.20 0.20 0.20 0.20 quaternary ammonium Fragrance 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0, 05 0.05 0.05 0.05 0.05 0.05 0.05 Wetting agent 0.30 0.30 0.30 0.30 amine Oxide ethoxylated cationic surfactant Quat dicoco Alcohol 0.20 0.20 0 , 20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 ethoxylated Tri-0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 ethanolamine NaEDTA 0.10 0.10 0.10 metasilicate 0.10 0.10 sodium pentahydrate 0.10 0.10 sodium carbonate Water * BBBBBBBBBBBBBBB * B means rest of water

Table 8 - General purpose cleaner formulations

33/38 Component A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 (% (% (% (% (% (% (% (% (% (% (% (% (% (% (% em em em em em em em em em em em weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) Polyethyl- 1.0 1 , 0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.20 1.0 1.20 1.0 oxazoline Compound 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.50 0.50 0.40 0.80 0.40 quaternary ammonium Fragrance 0.10 0.10 0, 10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Oxide 0.90 0.90 0.90 0, 90 0.90 0.90 0.90 0.60 0.45 0.45 0.60 0.60 0.60 0.45 amine Alcohol 0.50 ethoxylated 1 Alcohol ethoxylated 2 Alkyl-polyglucoside Tri- 1.0 ethanolamine 5.00 glycol ether 1 Glycol ether 2 NaEDTA 0.40 Metasilicate 0.10 0.25 0.25 0.25 0.10 0.10 0.10 0.10 sodium pentahydrate

0.10 sodium carbonate STPP 0.10 TKPP 0.10 Water * B B B B B B B B B B B B B B

Component A16 A17 A18 A19 A20 A21 A22 A23 A24 A25 A26 A27 A28 A29 A30 A31 A32 (% (% (% (% (% (% (% (% (% (% (% (% (% (% (% (% (% (% em em em em em em em em em em em em em em em weight weight weight weight weight weight weight weight weight weight weight weight weight weight weight weight weight)))))))))))))))) ))) Polyethyl- 1.0 0.80 0.80 1.0 1.0 1.20 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 oxazoline Compound of 0.80 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0, 50 0.50 0.50 0.50 quaternary ammonium Fragrance 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0 , 10 0.10 0.10 0.10 0.10 Oxide 0.60 0.60 0.60 1.5 1.2 0.60 0.60 amine Alcohol 0.10 0.20 0.60 0, 60 0.80 ethoxylated 1 Alcohol 0.10 0.20 0.20 0.80 ethoxylated 2 Alkyl- 0.60 0.50 0.50 0.40 0.40 0.40 polyglucoside o Tri-0.50 0, 50 0.50 0.50 0.50 0.50 0.50 ethanolamine 5.00 ether 2.40 2.40 glycol 1

34/38 Ether of 2.40 2.40 2.40 glycol 2 NaEDTA Metasilicate 0.10 0.10 0.05 0.05 0.05 0.05 0.05 sodium pentahydrate Sodium carbonate

STPP

TKPP Water * BBBBBBBBBBBBBBBBB Table 9 - Bathroom cleaner formulations Comp B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 B13 B14 B15 (% (% (% (% (% (% (% (% (% (% % (% (% (% (% (% em em em em em em em em em em em em em em by weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) weight) Polyethyl- 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1, 0 oxazoline Compound 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 of ammonium Quaternary Fragrance 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Oxide of 0 , 84 0.42 0.84 0.42 0.84 0.42 0.84 0.42 0.84 0.42 amine Alcohol 0.84 0.84 0.84 0.84 0.84 ethoxylated 1 Alcohol 0 , 50 0.50 0.50 0.50 0.50 ethoxylated 2 4.00 ether 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 glycol NaEDTA 2.90 2.90 2.90 2.90 2.90 2.90 Acid 2.50 2.50 2.50 2.50 2.50 2.50 Citric Acid 2.50 2.50 2.50 Sulfamic Water + * BBBBBBBBBBBBBBB Component B16 B17 B18 B19 B20 B 21 B22 B23 B24 (% in (% in (% in (% in (% in (% in (% in (% by weight) weight) weight) weight) weight) weight) weight) weight) weight) Polyethyl - 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Oxazoline Compound of 0.80 0.80 0.80 0.80 0.80 0.80 0 , 80 0.80 0.80 quaternary ammonium Fragrance 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Oxide of 0.80 0.40 0.80 0 , 40 0.80 0.40 amine Alcohol 0.80 0.80 0.80 ethoxylated 1 Alcohol 0.50 0.50 0.50 ethoxylated 2 4.0 4.0 4.0 4.0 4.0 glycol NaEDTA Acid citrus 2.50 2.50 2.50 2.50 2.50 2.50

35/38 Sulfuric acid 2.50 2.50 2.50 Water * B B B B B B B B B

[00144] A concentrate from a water-based formulation is prepared by removing a portion of the water from the formulation, concentrating the remaining raw materials. The resulting concentrate can then be diluted again to use resistance before use. Concentrates 5 times and 10 times are typically used in the cleaning and sanitization industries with customers in the hospital area. Concentrates can be prepared 15 times, 20 times or even higher levels, as desired, and still be within the scope of the present invention. Concentrates are desirable in these industries as they reduce transportation costs and conserve storage space. Table 10 illustrates examples of formulations of concentrate formulations in one embodiment of the invention. Table 10 - Concentrate formulations Component Protector Protector Protector Cleaner Cleaner Cleaner Cleaner Cleaner 5x 10x 20x use of general use 5x general use 10x general 20x bathroom toilet bathroom 5x 10x 20x Polyethyloxazoli 5.0 10 20 5.0 10 20 5.0 10 20 na Compound of 1.0 2.0 4 2.5 5.0 10 1.6 3.2 6.4 quaternary ammonium Fragrance 0.25 0.50 1.0 0.5 1.0 2.0 0.75 1.5 3.0 Surfactant 0.50 1.0 2.0 4.0 8.0 16 3.0 6.0 12 Glycol ether 12.5 25 50 TEA 2.5 5, 0 10 Citric acid 12.5 25 50 Water * BBBBBBBBB Comparative Example

[00145] Multiple polymeric compounds were tested for the ability to provide persistent reduction of biological load using the EPA 01-1A protocol against Enterobacter aerogenes. 50 microL of each formulation was allowed to dry on glass carriers before being subjected to the test. Each test was conducted in the presence of 5% FBS. As noted in Table 11, the combination of polyoxazoline and quaternary ammonium provided a synergistic residual sanitization benefit.

36/38 Table 11 Formula 1 Formula 2 Formula 3 Formula 4 Formula 5 Ingredient Mixture Mixture Mixture Mixture Mixture of Active Ammonium Ammonium Ammonium Quaternary Quaternary Quaternary Quaternary Quaternary Quaternary Polymer 3 Glycidoxy-Trimethoxysilyl-emulsion polyoxazoline propyl acrylate propyl acrylic polyamide i- (Polyethyleneimin silane) SSP-060 Aesthetics cloudy film clear film, clear film, clear, non-sticky film non-sticky non-sticky sticky when dry Log reduction NT * NT * log reduction log reduction log reduction log reduction associated with 0.5 of 1.8 5 * indicates not tested (NT) due to poor aesthetics Example - Formulation of cleaning cloths

[00146] A-I cleaning cloth formulations were prepared and tested on various substrates.

[00147] In tests for formulations A-F, 8 grams of formulation were added to a 7 ”x 8” substrate, 100% PP, 34 grams per square meter. The substrate, which was obtained from Rockline Industries, was a Kimberly Clark fusion blown product. The results are shown in table 12 below. Table 12

ABCDEF (% by weight) (% by weight) (% by weight) (% by weight) (% by weight) (% by weight) Aquazol 1.50 1.50 1.50 0.60 0.90 1.20 500 BTC 885 0.45 0.90 1.43 1.00 1.00 1.00 (50% active) Tomadol 25-12 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Fragrance 0 , 10 0.10 0.10 0.10 0.10 0.10 Water rest rest rest rest rest Reduction * of Log 2.4 3.9 4.7 <0.5 2.8 3.2 Result * Failure Approved Approved Failure Failed Approved

[00148] * Results of the Clorox method 01-1A - The method was adapted for cleaning cloths by folding the substrate longitudinally in 1 ”folds then wrapping the folded substrate strip through the second, third and fourth fingers. A pass was made through the glass carriers. A log reduction greater than 3.0 was required to be considered approved.

37/38

[00149] In each of the tests for formulations G and H, 11 grams of a formulation was added to a US Nonwovens 7 ”x 8” 100% polyester substrate (Item # XNW-8045).

[00150] The results are shown in table 13 below. Table 13

GH (% by weight) (% by weight) Aquazol 500 1.50 1.50 BTC 885 1.00 1.40 (50% active) Tomadol 25-12 0.20 0.20 Fragrance 0.10 0.10 Water rest rest Log reduction * 2.2 4.1 Result * Failure Approved

[00151] * Results of the Clorox method 01-1A— The method was adapted for cleaning cloths by folding the substrate longitudinally in 1 ”folds and then wrapping the folded substrate strip through the second, third and fourth fingers. A pass was made through glass carriers. A log reduction greater than 3.0 was required to be considered approved.

[00152] In tests for formulation I, 8 grams of the formulation were added on a substrate 100% polypropylene (PP) of 7 ”x 8”, 35 grams per square meter. The substrate was Diamond cleaning cloths (Item # P50535). The results are shown in table 14 below. Table 14

I (% by weight) Aquazol 500 1.50 BTC 0.90 (50% active) Tomadol 25-12 0.20 Fragrance 0.10 Water rest Log reduction * 4.3 Result * Approved

[00153] * Results of the Clorox method 01-1A— The method was adapted for cleaning cloths by folding the substrate longitudinally in 1 ”folds and then wrapping the folded substrate strip through the second,

38/38 third and fourth fingers. A pass was made through glass carriers. A log reduction greater than 3.0 was required to be considered approved.

[00154] Therefore, it will be quickly understood by those skilled in the art that the present composition and methods are susceptible of wide utility and application. Many modalities other than those described here, as well as many variations, modifications and equivalent arrangements, will be apparent or reasonably suggested to those skilled in the art by the present description and the preceding description thereof, without deviating from the substance or scope thereof.

[00155] Consequently, although the present composition and methods have been described here in detail in relation to their preferred modality, it should be understood that this description is only illustrative and exemplary and is made only for the purpose of providing a complete and facilitating description.

[00156] The preceding description is not intended or should be interpreted to limit or otherwise exclude any other modalities, adaptations, variations, modifications and equivalent arrangements.

Claims (25)

1. Cleaning cloth having a residual biocidal property, the cleaning cloth characterized by the fact that it comprises: a substrate comprised of a fibrous material, and a formulation present on or in the substrate; wherein the formulation comprises a poly (2-ethyl-2-oxazoline), a quaternary ammonium compound or combination of quaternary ammonium compounds, a surfactant, water, and an optional fragrance. 2. Cleaning cloth according to claim 1, characterized by the fact that the substrate is water-absorbent, water-adsorbent, or both. 3. Cleaning cloth according to claim 1, characterized by the fact that the substrate is comprised of a material selected from the group consisting of a natural material, a synthetic material, and a combination thereof. 4. Cleaning cloth according to claim 3, characterized by the fact that the natural material is selected from the group consisting of cotton, linen, silk, wool, hemp, linen, and a combination thereof. 5. Cleaning cloth according to claim 3, characterized by the fact that the synthetic material is selected from the group consisting of polypropylene, polystyrene, polyester, nylon, rayon, acrylic, spandex, and a combination thereof. 6. Cleaning cloth according to claim 1, characterized by the fact that the substrate is woven or non-woven. 7. Cleaning cloth according to claim 1, characterized by the fact that non-woven substrate is selected from the group consisting of blown, co-formed, spunbonded, airway / airflow, hydroentralization, spunlaced, bonded, carded, laminated, and a combination of them. 8. Cleaning cloth according to claim 1, characterized by the fact that the quaternary ammonium compound or a combination of quaternary ammonium compounds is in a 1: 4 ratio of poly (2-ethyl-2-oxazoline) to 1: 1. 9. Cleaning cloth according to claim 1, characterized by the fact that poly (2-ethyl-2-oxazoline) is in an amount of 1% by weight to 5% by weight, and the percentage by weight is with based on the total weight of the formulation. 10. Cleaning cloth according to claim 1, characterized by the fact that the quaternary ammonium compound is in an amount of at least 0.2 weight percent, and the weight percentage is based on the total weight of the formulation . 11. Cleaning cloth according to claim 10, characterized by the fact that the quaternary ammonium compound is in an amount of 0.2 weight percent to 2 weight percent, and the weight percentage based on weight total formulation. 12. Cleaning cloth according to claim 1, characterized by the fact that the surfactant is a non-ionic surfactant. 13. Cleaning cloth according to claim 1, characterized by the fact that the surfactant is an alcohol ethoxylate. Cleaning cloth according to claim 13, characterized in that the alcohol ethoxylate is in an amount of 0.05% by weight to 0.5% by weight, and the percentage by weight is based on weight total formulation. 15. Cleaning cloth according to claim 13, characterized by the fact that alcohol ethoxylate is a linear alcohol ethoxylate with at least 9 moles of ethylene oxide. 16. Cleaning cloth according to claim 1, characterized by the fact that the fragrance is in an amount from 0% by weight to 0.5% by weight, and the percentage by weight is based on the total weight of the formulation. 17. Cleaning cloth according to claim 1, characterized in that the formulation additionally comprises an alcohol, a salt, an acid, or a combination thereof. 18. Cleaning cloth having a residual biocidal property, the cleaning cloth characterized by the fact that it comprises: a substrate comprised of a fibrous material, and a formulation present on or in the substrate; wherein the formulation comprises: 1% by weight to 2% by weight of a poly (2-ethyl-2-oxazoline), at least 0.4% by weight of a quaternary ammonium compound or a combination of quaternary ammonium compounds 0.1% by weight to 0.3% by weight of an alcohol ethoxylate with at least 9 moles of ethylene oxide, and a remainder of water, in which weight percentages are based on the total weight of the formulation. 19. Cleaning cloth according to claim 18, characterized by the fact that the substrate is comprised of a material selected from the group consisting of a natural material, a synthetic material, and a combination thereof. 20. Cleaning cloth according to claim 19, characterized by the fact that the synthetic material is selected from the group consisting of polypropylene, polystyrene, polyester, nylon, rayon, acrylic, spandex, and a combination thereof. 21. Cleaning cloth according to claim 18, characterized in that the alcohol ethoxylate has at least 12 moles of ethylene oxide. 22. Cleaning cloth according to claim 18, characterized in that it additionally comprises 0.05 weight percent to 0.2 weight percent of a fragrance. 23. Disinfectant formulation, characterized by the fact that it comprises: 1% by weight to 2% by weight of a poly (2-ethyl-2-oxazoline), at least 0.4% by weight of a quaternary ammonium compound or a combination of quaternary ammonium compounds, 0.1% by weight to 0.3% by weight of an alcohol ethoxylate with at least 9 moles of ethylene oxide, and a remainder of water, in which weight percentages are based on total formulation weight, and where the formulation provides up to 24 hours of residual sanitizing activity. 24. Disinfectant formulation according to claim 23, characterized in that the alcohol ethoxylate has at least 12 moles of ethylene oxide. 25. Disinfectant formulation, characterized by the fact that it comprises: a polymer binder in a range of 0.1% to 20% based on the weight of the disinfectant formulation, in which the polymer binder is an oxazoline homopolymer or an extended polymer or a modified one based on an oxazoline homopolymer, a biocidal compound in a range of 0.05% to 20% based on the weight of the disinfectant formulation, a surfactant in a range of 0.01% to 20% based on the weight of the disinfectant formulation, a pH adjusting agent in a range of 0.01% to 10% based on the weight of the disinfectant formulation, glycol ether in a range of 1% to 50% based on the weight of the disinfectant formulation, and water in a range of 0% to 99.9% based on the weight of the disinfectant formulation.