BR112018003576A2 - aumentando teor de óleo de planta através de alteração de um regulador negativo de acetil coa carboxilase - Google Patents

aumentando teor de óleo de planta através de alteração de um regulador negativo de acetil coa carboxilase

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Publication number
BR112018003576A2
BR112018003576A2 BR112018003576A BR112018003576A BR112018003576A2 BR 112018003576 A2 BR112018003576 A2 BR 112018003576A2 BR 112018003576 A BR112018003576 A BR 112018003576A BR 112018003576 A BR112018003576 A BR 112018003576A BR 112018003576 A2 BR112018003576 A2 BR 112018003576A2
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BR
Brazil
Prior art keywords
regulator
negative
changing
oil content
coa carboxylase
Prior art date
Application number
BR112018003576A
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English (en)
Inventor
Thelen Jay
Salie Matthew
Original Assignee
Univ Missouri
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Univ Missouri filed Critical Univ Missouri
Publication of BR112018003576A2 publication Critical patent/BR112018003576A2/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

a presente invenção refere-se ao provimento de um método e meios para alterar produção de ácido graxo e por último triacil glicerol emplantas e algas. métodos da invenção compreendem a etapa de alteração de níveis de atividade da etapa realizada para biossíntese de ácido graxo de novo. acetil-coa carboxilases (accase). mais especificamente, métodos da invenção aperfeiçoam diretamente a atividade de accase através de regulaçãodescendente de genes contendo domínio de ligação de biotina / lipoíla (badc) através de abrodagens de biotecnologia ou cultivo seletivo.
BR112018003576A 2015-08-28 2016-07-07 aumentando teor de óleo de planta através de alteração de um regulador negativo de acetil coa carboxilase BR112018003576A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562211371P 2015-08-28 2015-08-28
PCT/US2016/041386 WO2017039834A1 (en) 2015-08-28 2016-07-07 Increasing plant oil content by altering a negative regulator of acetyl-coa carboxylase

Publications (1)

Publication Number Publication Date
BR112018003576A2 true BR112018003576A2 (pt) 2018-09-25

Family

ID=58188729

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018003576A BR112018003576A2 (pt) 2015-08-28 2016-07-07 aumentando teor de óleo de planta através de alteração de um regulador negativo de acetil coa carboxilase

Country Status (7)

Country Link
US (2) US10883113B2 (pt)
EP (1) EP3341486A4 (pt)
CN (2) CN116121294A (pt)
AU (1) AU2016317094B2 (pt)
BR (1) BR112018003576A2 (pt)
CA (1) CA2995573A1 (pt)
WO (1) WO2017039834A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116121294A (zh) 2015-08-28 2023-05-16 密苏里大学管理者 通过改变乙酰-coa羧化酶的负调控因子来增加植物油含量
US11802286B2 (en) * 2016-07-07 2023-10-31 The Curators Of The University Of Missouri Increasing plant oil content by improving activity of acetyl-CoA carboxylase
CA3101203A1 (en) * 2018-05-30 2019-12-05 The Curators Of The University Of Missouri Methods of altering seed weight and seed oil content by manipulating alpha-carboxyltransferase (a-ct) activity via carboxyltransferase interactor (cti) protein expression
CN116064654A (zh) * 2020-08-06 2023-05-05 中国农业科学院棉花研究所 GbBCCP5蛋白质及其编码基因在调控生物油脂含量中的应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9306490D0 (en) 1993-03-29 1993-05-19 Zeneca Ltd Plant gene specifying acetyl,coenzyme a carboxylase and transformed plants containing same
WO1998005758A1 (en) 1996-08-02 1998-02-12 Board Of Trustees Operating Michigan State University STRUCTURE AND EXPRESSION OF THE ALPHA-CARBOXYLTRANSFERASE SUBUNIT OF HETEROMERIC-ACETYL-CoA CARBOXYLASE
US20110281765A1 (en) * 2000-03-29 2011-11-17 Bush David F Plant polymorphic markers and uses thereof
JP3656104B2 (ja) 2001-03-13 2005-06-08 国立大学法人 奈良先端科学技術大学院大学 植物において脂肪酸合成を促進させる方法
US20030110533A1 (en) * 2001-07-06 2003-06-12 Cahoon Rebecca E. Acetyl-CoA carboxylase subunits
AU2001289843A1 (en) * 2001-08-28 2002-02-13 Bayer Cropscience Ag Polypeptides for identifying herbicidally active compounds
DE102005013779A1 (de) * 2005-03-22 2006-09-28 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen
MX2009010858A (es) * 2007-04-09 2009-11-02 Evogene Ltd Polinucleotidos, polipeptidos y metodos para aumentar el contenido de aceite, la velocidad de crecimiento y biomasa de las plantas.
PL222498B1 (pl) 2009-05-27 2016-08-31 Inst Hodowli I Aklimatyzacji Roślin Kaseta DNA, wektor binarny, szczep A. tumefaciens oraz sposób otrzymywania rośliny zbożowej o zwiększonej produktywności
CA2840525A1 (en) * 2011-06-28 2013-01-03 Brookhaven Science Associates, Llc Modified plants with increased oil content
CN116121294A (zh) 2015-08-28 2023-05-16 密苏里大学管理者 通过改变乙酰-coa羧化酶的负调控因子来增加植物油含量

Also Published As

Publication number Publication date
US11959087B2 (en) 2024-04-16
US20180251775A1 (en) 2018-09-06
EP3341486A1 (en) 2018-07-04
US10883113B2 (en) 2021-01-05
AU2016317094B2 (en) 2021-12-16
CN116121294A (zh) 2023-05-16
US20210171970A1 (en) 2021-06-10
EP3341486A4 (en) 2019-05-15
CA2995573A1 (en) 2017-03-09
CN108699568A (zh) 2018-10-23
WO2017039834A1 (en) 2017-03-09
AU2016317094A1 (en) 2018-03-08

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