BR112017006215A2 - método para detectar uma proximidade espacial de um primeiro e um segundo epítopos, método para estratificação de um indivíduo, kit para realizar o método, e, uso do kit - Google Patents

método para detectar uma proximidade espacial de um primeiro e um segundo epítopos, método para estratificação de um indivíduo, kit para realizar o método, e, uso do kit

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Publication number
BR112017006215A2
BR112017006215A2 BR112017006215A BR112017006215A BR112017006215A2 BR 112017006215 A2 BR112017006215 A2 BR 112017006215A2 BR 112017006215 A BR112017006215 A BR 112017006215A BR 112017006215 A BR112017006215 A BR 112017006215A BR 112017006215 A2 BR112017006215 A2 BR 112017006215A2
Authority
BR
Brazil
Prior art keywords
kit
spatial proximity
oligonucleotide
epitopes
individual
Prior art date
Application number
BR112017006215A
Other languages
English (en)
Inventor
Arnoldina Margaretha Wilhelmina Van Strijp Dianne
Van Hemert Freek
Wimberger-Friedl Reinhold
Original Assignee
Koninklijke Philips Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips Nv filed Critical Koninklijke Philips Nv
Publication of BR112017006215A2 publication Critical patent/BR112017006215A2/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in eptitope analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/56—Staging of a disease; Further complications associated with the disease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/70—Mechanisms involved in disease identification
    • G01N2800/7057—(Intracellular) signaling and trafficking pathways

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

a presente invenção refere-se a um método para detectar uma proximidade espacial de um primeiro e um segundo epítopos (11, 21) de uma proteína ou de uma primeira e uma segunda proteínas (10, 20) de um complexo de proteína (1) em uma amostra de um indivíduo. o método compreende ligar um primeiro membro de ligação (30), que tem um primeiro oligonucleotídeo (31) conjugado ao mesmo, ao primeiro epítopo (11), ligar um segundo membro de ligação (40), que tem um segundo oligonucleotídeo (41) conjugado ao mesmo, ao segundo epítopo (21), e determinar se um efeito de transferência de energia de ressonância por fluorescência (fret) está presente ou não entre um fluoróforo doador (32) e um fluoróforo receptor (42), os quais estão associados ao primeiro oligonucleotídeo (31) e ao segundo oligonucleotídeo (41), sendo que a presença do efeito de fret indica uma proximidade espacial do primeiro e do segundo oligonucleotídeos (31, 41) e, dessa forma, a proximidade espacial do primeiro e do segundo epítopos (11, 21).
BR112017006215A 2014-09-30 2015-09-30 método para detectar uma proximidade espacial de um primeiro e um segundo epítopos, método para estratificação de um indivíduo, kit para realizar o método, e, uso do kit BR112017006215A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14187001 2014-09-30
PCT/EP2015/072495 WO2016050813A1 (en) 2014-09-30 2015-09-30 Method for detecting a spatial proximity of a first and a second epitope

Publications (1)

Publication Number Publication Date
BR112017006215A2 true BR112017006215A2 (pt) 2018-03-06

Family

ID=51628033

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017006215A BR112017006215A2 (pt) 2014-09-30 2015-09-30 método para detectar uma proximidade espacial de um primeiro e um segundo epítopos, método para estratificação de um indivíduo, kit para realizar o método, e, uso do kit

Country Status (7)

Country Link
US (1) US20170234887A1 (pt)
EP (1) EP3201629A1 (pt)
JP (1) JP6640843B2 (pt)
CN (1) CN106715724A (pt)
BR (1) BR112017006215A2 (pt)
RU (1) RU2717956C2 (pt)
WO (1) WO2016050813A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108956991B (zh) * 2018-07-25 2021-04-27 南通大学 一种荧光共振能量转移生物传感器及其应用
CN115112880B (zh) * 2022-06-23 2023-12-22 南京浦光生物科技有限公司 检测小分子物质的试剂组合、试剂盒、检测系统及检测方法
CN115112902B (zh) * 2022-06-23 2024-05-24 南京浦光生物科技有限公司 检测目标蛋白的试剂组合、试剂盒、检测系统及检测方法
NL2032916B1 (en) * 2022-08-31 2024-03-15 Univ Delft Tech Single-molecule aptamer FRET for protein identification and structural analysis

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
CA2545006C (en) * 2003-12-12 2013-09-17 Saint Louis University Biosensors for detecting macromolecules and other analytes
US9173573B2 (en) * 2005-02-23 2015-11-03 Koninklijke Philips N.V. Imaging an object of interest
JP5088034B2 (ja) * 2006-08-14 2012-12-05 ソニー株式会社 物質の検出等に有用な核酸鎖とその方法
JP2011505549A (ja) * 2007-11-21 2011-02-24 エラスムス ユニバーシティ メディカル センター ロッテルダム 改良型ｆｒｅｔプローブおよびその使用
EP2304436A1 (en) * 2008-07-10 2011-04-06 Nodality, Inc. Methods for diagnosis, prognosis and treatment
WO2011022820A1 (en) * 2009-08-31 2011-03-03 The Governors Of The University Of Alberta Binding-induced hairpin detection system
WO2011106583A1 (en) * 2010-02-26 2011-09-01 Ventana Medical Systems, Inc. Polytag probes
GB201107863D0 (en) * 2011-05-11 2011-06-22 Olink Ab Method and product
WO2014071070A1 (en) * 2012-11-01 2014-05-08 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
WO2014165210A2 (en) * 2013-03-12 2014-10-09 Life Technologies Corporation Universal reporter-based genotyping methods and materials

Also Published As

Publication number Publication date
EP3201629A1 (en) 2017-08-09
RU2717956C2 (ru) 2020-03-27
JP2017533417A (ja) 2017-11-09
US20170234887A1 (en) 2017-08-17
JP6640843B2 (ja) 2020-02-05
WO2016050813A1 (en) 2016-04-07
CN106715724A (zh) 2017-05-24
RU2017114633A (ru) 2018-11-05
RU2017114633A3 (pt) 2019-05-07

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2690 DE 26-07-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.