BR112016024945A2 - edição de gene multiplex em suínos - Google Patents

edição de gene multiplex em suínos

Info

Publication number
BR112016024945A2
BR112016024945A2 BR112016024945A BR112016024945A BR112016024945A2 BR 112016024945 A2 BR112016024945 A2 BR 112016024945A2 BR 112016024945 A BR112016024945 A BR 112016024945A BR 112016024945 A BR112016024945 A BR 112016024945A BR 112016024945 A2 BR112016024945 A2 BR 112016024945A2
Authority
BR
Brazil
Prior art keywords
swine
gene editing
multiplex gene
multiplex
editing
Prior art date
Application number
BR112016024945A
Other languages
English (en)
Portuguese (pt)
Inventor
f carlson Daniel
C Fahrenkrug Scott
Original Assignee
Recombinetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Recombinetics Inc filed Critical Recombinetics Inc
Priority to BR122023023211-9A priority Critical patent/BR122023023211A2/pt
Publication of BR112016024945A2 publication Critical patent/BR112016024945A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR112016024945A 2014-04-28 2015-04-28 edição de gene multiplex em suínos BR112016024945A2 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR122023023211-9A BR122023023211A2 (pt) 2014-04-28 2015-04-28 Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461985327P 2014-04-28 2014-04-28
PCT/US2015/027995 WO2015168125A1 (en) 2014-04-28 2015-04-28 Multiplex gene editing in swine

Publications (1)

Publication Number Publication Date
BR112016024945A2 true BR112016024945A2 (pt) 2017-10-24

Family

ID=53190022

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112016024945A BR112016024945A2 (pt) 2014-04-28 2015-04-28 edição de gene multiplex em suínos
BR122023023211-9A BR122023023211A2 (pt) 2014-04-28 2015-04-28 Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR122023023211-9A BR122023023211A2 (pt) 2014-04-28 2015-04-28 Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano

Country Status (13)

Country Link
US (4) US20160029604A1 (https=)
EP (1) EP3136850A1 (https=)
JP (1) JP2017513510A (https=)
KR (1) KR20170003585A (https=)
CN (2) CN111647627A (https=)
AP (1) AP2016009588A0 (https=)
AR (1) AR100216A1 (https=)
AU (1) AU2015253352A1 (https=)
BR (2) BR112016024945A2 (https=)
CA (1) CA2946881A1 (https=)
MX (1) MX2016014066A (https=)
PH (1) PH12016502162A1 (https=)
WO (1) WO2015168125A1 (https=)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
KR101566498B1 (ko) * 2013-11-13 2015-11-06 건국대학교 산학협력단 인터루킨 2 수용체 감마 유전자 적중벡터, 그 벡터가 도입된 면역세포 결핍 형질전환 미니 복제돼지 생산과 그 제조방법 및 활용
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
BR112016024945A2 (pt) * 2014-04-28 2017-10-24 Recombinetics Inc edição de gene multiplex em suínos
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
WO2016049182A1 (en) 2014-09-23 2016-03-31 Aggentics, Inc. Materials and methods for producing animals with short hair
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP4464338A3 (en) 2014-11-07 2025-02-12 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
KR102684690B1 (ko) 2015-03-03 2024-07-15 리젠츠 오브 더 유니버시티 오브 미네소타 Etv2 및 그의 용도
WO2017004388A1 (en) * 2015-06-30 2017-01-05 Regents Of The University Of Minnesota Humanized heart muscle
RU2018103093A (ru) * 2015-06-30 2019-07-31 Реджентс Оф Зэ Юниверсити Оф Миннесота Гуманизированная скелетная мышца
EP3353296B1 (en) 2015-09-24 2020-11-04 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
US20170099813A1 (en) * 2015-10-07 2017-04-13 Recombinetics, Inc. Method of generating sterile terminal sires in livestock and animals produced thereby
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
CN105567689B (zh) * 2016-01-25 2019-04-09 重庆威斯腾生物医药科技有限责任公司 CRISPR/Cas9靶向敲除人TCAB1基因及其特异性gRNA
US11597924B2 (en) 2016-03-25 2023-03-07 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
AU2017252023B2 (en) * 2016-04-18 2024-05-02 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of hemoglobinopathies
CN106191061B (zh) * 2016-07-18 2019-06-18 暨南大学 一种特异靶向人ABCG2基因的sgRNA导向序列及其应用
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US20180084767A1 (en) * 2016-09-21 2018-03-29 Recombinetics, Inc. Animal models for cardiomyopathy
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018094291A1 (en) * 2016-11-18 2018-05-24 Christopher Bradley Massively multiplexed homologous template repair for whole-genome replacement
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3583203B1 (en) * 2017-02-15 2023-11-01 2seventy bio, Inc. Donor repair templates multiplex genome editing
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
CN106987604B (zh) * 2017-03-29 2021-05-28 北京希诺谷生物科技有限公司 一种制备动脉粥样硬化疾病模型犬的方法
CN110476214B (zh) * 2017-03-30 2024-08-27 孟山都技术有限公司 用于鉴定多个基因组编辑和预测鉴定的基因组编辑的集总效应的系统和方法
KR102746223B1 (ko) 2017-04-20 2024-12-27 이제네시스, 인크. 유전자 변형 동물의 생성 방법
WO2018197520A1 (en) * 2017-04-24 2018-11-01 Dupont Nutrition Biosciences Aps Methods and compositions of anti-crispr proteins for use in plants
CN107974462A (zh) * 2017-04-28 2018-05-01 中山大学 一种通过延迟基因的表达时间来提高猪瘦肉产量的方法
CN107182940B (zh) * 2017-05-09 2020-05-15 中国科学院动物研究所 一种抗寒及瘦肉型转基因猪及其制备方法
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
CN107937445B (zh) * 2017-07-25 2021-05-04 北京希诺谷生物科技有限公司 利用体细胞克隆技术制备基因敲除犬的方法
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
US20190045761A1 (en) 2017-08-11 2019-02-14 Recombinetics, Inc. Inducible disease models methods of making them and use in tissue complementation
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
KR102207352B1 (ko) * 2017-09-29 2021-01-26 서울대학교산학협력단 Klotho 유전자 넉아웃 질환모델 동물 및 이의 용도
EP3694561A4 (en) * 2017-10-12 2021-08-04 Mayo Foundation for Medical Education and Research METHODS AND MATERIALS FOR GENEDITATION
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
AU2018378479B2 (en) 2017-12-05 2025-03-13 Vertex Pharmaceuticals Incorporated CRISPR-Cas9 modified CD34+ human hematopoietic stem and progenitor cells and uses thereof
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US11566236B2 (en) 2018-02-05 2023-01-31 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of hemoglobinopathies
CN108504685A (zh) * 2018-03-27 2018-09-07 宜明细胞生物科技有限公司 一种利用CRISPR/Cas9系统同源重组修复IL-2RG缺陷基因的方法
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
KR102103103B1 (ko) 2018-08-16 2020-04-21 (주)라트바이오 인위적 뉴클레아제를 생산하는 형질전환 동물 및 형질전환 배아
CN110846330B (zh) * 2018-08-21 2021-08-27 中国科学院动物研究所 一种突变基因及其用于构建蹼足病小型猪模型的用途
CN109321600A (zh) * 2018-10-19 2019-02-12 中国农业大学 一种培育生产低致敏性牛奶的牛的方法及其应用
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
CN109735630A (zh) * 2019-01-05 2019-05-10 兰州大学 绵羊zfy基因单核苷酸多态性标记的检测方法及标记应用
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
CN111484994B (zh) * 2019-01-29 2022-04-19 四川大学华西医院 CRISPR-Cas9特异性敲除猪Fah、Rag2双基因的方法
CN109777834A (zh) * 2019-02-25 2019-05-21 中国科学院广州生物医药与健康研究院 一种重度免疫缺陷猪模型及其构建方法和应用
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US11859213B2 (en) 2019-05-16 2024-01-02 Regents Of The University Of Minnesota Development of superior chimerism by hiPSC engineering and embryo aggregation
CN110452929B (zh) * 2019-07-09 2021-07-20 中山大学 一种非嵌合基因编辑猪胚胎模型的构建方法
WO2021011561A1 (en) * 2019-07-15 2021-01-21 The General Hospital Corporation Zebrafish deletion and compound mutants and uses thereof
CN110305872A (zh) * 2019-07-17 2019-10-08 中国农业科学院北京畜牧兽医研究所 小型猪2型糖尿病模型的构建方法及应用
EP4013859A4 (en) * 2019-08-15 2023-10-11 The Rockefeller University CRISPR-BASED GENOME EDITING WITH CELL SURFACE DISPLAY TO PRODUCE HOMOZYGOTICLY ENGINEERED EUKARYOTIC CELLS
CN112538497B (zh) * 2019-09-20 2023-02-28 南京启真基因工程有限公司 CRISPR/Cas9系统及其在构建α、β和α＆β地中海贫血模型猪细胞系中的应用
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CA3158963A1 (en) 2019-11-22 2021-05-27 Samir Mitragotri Ionic liquids for drug delivery
KR102270145B1 (ko) * 2019-12-27 2021-07-01 서울대학교산학협력단 Btk 넉아웃 질환모델용 돼지 및 이의 용도
WO2021150712A1 (en) * 2020-01-21 2021-07-29 The Board Of Regents Of The University Of Texas System Modulating tlr/nf-kb and p53 signaling pathways to enhance interspecies chimerism between evolutionary distant species
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
CN111334529B (zh) * 2020-05-20 2020-09-29 中国农业大学 一种利用第三代碱基编辑器制备精准blg基因敲除牛的方法
CN111534519B (zh) * 2020-05-26 2021-12-14 中国农业科学院北京畜牧兽医研究所 识别猪eIF4G1基因的sgRNA及其编码DNA和应用
WO2021247672A1 (en) * 2020-06-02 2021-12-09 Catalent Pharma Solutions, Llc Nucleic acid constructs for protein manufacture
JP7849796B2 (ja) * 2020-09-10 2026-04-22 Jsr株式会社 オリゴデンドロサイト様細胞の製造方法
CN113234722B (zh) * 2021-04-30 2023-03-14 肇庆市瑞思元生物科技有限公司 利用碱基编辑修复与板层状鱼鳞病相关的tgm1 c607t突变的试剂和方法
CN113583944B (zh) * 2021-09-15 2023-07-21 广西壮族自治区水牛研究所 激活Wnt/β-catenin信号通路在提高水牛体外胚胎生产效率中的应用
EP4412587A1 (en) 2021-10-08 2024-08-14 President And Fellows Of Harvard College Ionic liquids for drug delivery
WO2023077360A1 (zh) * 2021-11-04 2023-05-11 云南农业大学 一种构建重症免疫缺陷和肝损伤双重猪模型的方法及应用
US20250300846A1 (en) * 2021-12-17 2025-09-25 Laurie Schmelzle Systems and methods for generating, managing, and displaying non-fungible digital representations of living, biological, and/or eukaryotic organisms
JP2023131904A (ja) * 2022-03-10 2023-09-22 国立大学法人愛媛大学 細胞の移植方法
CN115976018B (zh) * 2022-08-26 2025-12-30 南京启真基因工程有限公司 构建abca12基因突变的斑色鱼鳞癣模型猪核移植供体细胞的试剂盒及其应用
CN115341044A (zh) * 2022-10-19 2022-11-15 佛山科学技术学院 一种利用微生物及其相关snp位点预测猪日增重的方法
CN115786343B (zh) * 2022-11-17 2024-08-30 石河子大学 一种猪Zfy基因的RNA干扰片段、表达载体及其应用
CN116334087A (zh) * 2023-05-10 2023-06-27 山东农业大学 特异性靶向猪Pax3基因的sgRNA序列及其应用
CN116790604B (zh) * 2023-08-18 2023-10-27 成都中科奥格生物科技有限公司 一种sgRNA、CRISPR/Cas9载体及其构建方法和用途
CN117982624B (zh) * 2024-02-04 2026-01-27 中国科学院水生生物研究所 肝细胞核因子HNF4β在制备预防和/或治疗鱼类疾病渔用生物制品中的应用
CN118910057B (zh) * 2024-09-04 2025-03-21 江苏农牧科技职业学院 用于敲除猪RBM20基因部分保守序列的sgRNA组合、猪模型和构建方法
CN119432858A (zh) * 2025-01-13 2025-02-14 江苏省农业科学院 一种用于精准编辑奶牛BLG基因的sgRNA组合、引物及应用
CN120204361B (zh) * 2025-03-28 2026-02-03 中国科学院水生生物研究所 Hnf4α或含Hnf4α的生物材料在制备抑制细菌和/或病毒产品中的应用

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
EP0821070A1 (en) 1996-07-22 1998-01-28 Carelli, Claude Marcel Henri Pit-1 gene polymorphism and trait selection in animals
GB2365445A (en) 1998-10-12 2002-02-20 Geron Bio Med Ltd Porcine occcytes with improved developmental competence
WO2001030965A2 (en) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US7468248B2 (en) 2002-12-31 2008-12-23 Cargill, Incorporated Methods and systems for inferring bovine traits
US7985739B2 (en) 2003-06-04 2011-07-26 The Board Of Trustees Of The Leland Stanford Junior University Enhanced sleeping beauty transposon system and methods for using the same
US20050153317A1 (en) 2003-10-24 2005-07-14 Metamorphix, Inc. Methods and systems for inferring traits to breed and manage non-beef livestock
AU2005237494B2 (en) * 2004-04-22 2010-06-10 Kyowa Hakko Kirin Co., Ltd Transgenic animals and uses thereof
CA2958259C (en) * 2004-10-22 2020-06-30 Revivicor, Inc. Ungulates with genetically modified immune systems
US20070033666A1 (en) 2005-06-24 2007-02-08 Harris Reuben S Using cytosine deaminases to diminish retroelement transfer from pigs to humans
AU2008305590A1 (en) * 2007-09-27 2009-04-02 Sangamo Therapeutics, Inc. Genomic editing in zebrafish using zinc finger nucleases
WO2010008562A2 (en) 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
US20110023159A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Ovine genome editing with zinc finger nucleases
EP2352369B1 (en) * 2008-12-04 2017-04-26 Sangamo BioSciences, Inc. Genome editing in rats using zinc-finger nucleases
US20110023140A1 (en) 2008-12-04 2011-01-27 Sigma-Aldrich Co. Rabbit genome editing with zinc finger nucleases
US20110030072A1 (en) * 2008-12-04 2011-02-03 Sigma-Aldrich Co. Genome editing of immunodeficiency genes in animals
KR20100080068A (ko) 2008-12-31 2010-07-08 주식회사 툴젠 신규한 징크 핑거 뉴클레아제 및 이의 용도
CN102858985A (zh) * 2009-07-24 2013-01-02 西格马-奥尔德里奇有限责任公司 基因组编辑方法
US20120192298A1 (en) 2009-07-24 2012-07-26 Sigma Aldrich Co. Llc Method for genome editing
WO2011017315A2 (en) 2009-08-03 2011-02-10 Recombinetics, Inc. Methods and compositions for targeted gene modification
US8518392B2 (en) 2009-08-14 2013-08-27 Regeneron Pharmaceuticals, Inc. Promoter-regulated differentiation-dependent self-deleting cassette
WO2011072246A2 (en) 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
EP2660318A1 (en) * 2010-02-09 2013-11-06 Sangamo BioSciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
EP2533629B1 (en) 2010-02-11 2018-11-28 Recombinetics, Inc. Methods and materials for producing transgenic artiodactyls
JP5841322B2 (ja) 2010-04-22 2016-01-13 オレゴン ヘルス アンド サイエンス ユニバーシティ フマリルアセト酢酸ヒドロラーゼ（ｆａｈ）欠損性ブタおよびその使用
MX2012012211A (es) * 2010-04-23 2012-12-17 Du Pont Composiciones de interruptores genicos y metodos de uso de las mismas.
EP2392208B1 (en) 2010-06-07 2016-05-04 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Fusion proteins comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use
CN103228130B (zh) * 2010-06-17 2016-03-16 科马布有限公司 动物模型及治疗分子
US9512444B2 (en) * 2010-07-23 2016-12-06 Sigma-Aldrich Co. Llc Genome editing using targeting endonucleases and single-stranded nucleic acids
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
MX363013B (es) 2011-02-25 2019-03-04 Recombinetics Inc Animales genéticamente modificados y métodos para su obtención.
AU2012249390B2 (en) 2011-04-27 2017-03-30 Amyris, Inc. Methods for genomic modification
WO2013123365A1 (en) 2012-02-15 2013-08-22 Mice With Horns, Llc Transgenic animals with customizable traits
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
AR091482A1 (es) * 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
US10058078B2 (en) 2012-07-31 2018-08-28 Recombinetics, Inc. Production of FMDV-resistant livestock by allele substitution
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
AP2015008495A0 (en) 2012-10-30 2015-05-31 Ct For Aquaculture Technologies Inc Control of sexual maturation in animals
MX377561B (es) 2012-11-05 2025-03-10 Regeneron Pharma Animales no humanos genéticamente modificados y métodos de uso de los mismos.
PL3360964T3 (pl) 2012-12-06 2020-03-31 Sigma-Aldrich Co. Llc Modyfikacja i regulacja genomu oparta na crispr
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP2840140B2 (en) 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
DK2931891T3 (da) 2012-12-17 2019-08-19 Harvard College Rna-styret modificering af menneskelige genomer
WO2014143381A1 (en) * 2013-03-09 2014-09-18 Agilent Technologies, Inc. Methods of in vivo engineering of large sequences using multiple crispr/cas selections of recombineering events
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
CA2908403A1 (en) 2013-04-02 2014-10-09 Bayer Cropscience Nv Targeted genome engineering in eukaryotes
US20150013025A1 (en) 2013-05-19 2015-01-08 Mice With Horns, Llc Transgenic animals with customizable traits
US20140359796A1 (en) 2013-05-31 2014-12-04 Recombinetics, Inc. Genetically sterile animals
US20140359795A1 (en) 2013-05-31 2014-12-04 Recombinetics, Inc. Genetic techniques for making animals with sortable sperm
WO2014197748A2 (en) 2013-06-05 2014-12-11 Duke University Rna-guided gene editing and gene regulation
ES2929143T3 (es) 2013-07-09 2022-11-25 Harvard College Ingeniería genómica guiada por ARN múltiplex
WO2015009571A1 (en) 2013-07-13 2015-01-22 Mice With Horns, Llc Improved methods for spermatogonial stem cell (ssc) transfer
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US20150064149A1 (en) 2013-09-04 2015-03-05 Mice With Horns, LLC. Materials and methods for correcting recessive mutations in animals
WO2015052231A2 (en) 2013-10-08 2015-04-16 Technical University Of Denmark Multiplex editing system
US10787684B2 (en) 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
EP3690044B1 (en) 2014-02-11 2024-01-10 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering
BR112016024945A2 (pt) * 2014-04-28 2017-10-24 Recombinetics Inc edição de gene multiplex em suínos

Also Published As

Publication number Publication date
CN106535630A (zh) 2017-03-22
US20180235194A1 (en) 2018-08-23
AR100216A1 (es) 2016-09-21
CN111647627A (zh) 2020-09-11
EP3136850A1 (en) 2017-03-08
US20220056482A1 (en) 2022-02-24
CN106535630B (zh) 2020-04-24
AU2015253352A1 (en) 2016-12-08
CA2946881A1 (en) 2015-11-05
BR122023023211A2 (pt) 2024-01-23
US12070022B2 (en) 2024-08-27
WO2015168125A1 (en) 2015-11-05
JP2017513510A (ja) 2017-06-01
AP2016009588A0 (en) 2016-11-30
PH12016502162A1 (en) 2017-02-06
KR20170003585A (ko) 2017-01-09
MX2016014066A (es) 2017-05-03
US20160029604A1 (en) 2016-02-04
US20240381856A1 (en) 2024-11-21

Similar Documents

Publication Publication Date Title
BR112016024945A2 (pt) edição de gene multiplex em suínos
IL252386A0 (en) Directed RNA editing
EP3359661C0 (en) MULTIPLEXED GENOME EDITING
IL233902B (en) Propulsion system
EP3334584A4 (en) EXTRUDER
DK3167234T3 (da) Kølesystem
DK3140500T3 (da) Brøndkompletteringssystem
IL255626B (en) Nutrient system
DK3517268T3 (da) Læggeenhed
FI20155573A7 (fi) Aikajanakäyttöliittymä
DK3161247T3 (da) Brøndsystem
CL2016002165A1 (es) Compuestos de aminocarbonilcarbamato
IL249254A0 (en) Gene expression system
EP3342940A4 (en) Hybrid construction machine
EP3251522C0 (en) FUNCTIONAL FOOD
IT201600102119A1 (it) Seminatrice
DK2902983T3 (da) Trådløst system
FR3034825B1 (fr) Insert taraude
GB201517227D0 (en) Genetically-edited swine
DK3053873T3 (da) Gjordsystem
DE112016002291A5 (de) Gurtretter-System
ITBO20140104U1 (it) Raccordo per ventilazione
DE102014119374B8 (de) Beatmungssystem
FR3020448B1 (fr) Systeme de ventilation
DE112014006555A5 (de) Kommutierungsschaltung

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B154 Notification of filing of divisional application [chapter 15.50 patent gazette]

Free format text: O PEDIDO FOI DIVIDIDO NO BR122023023211-9 PROTOCOLO 870230098010 EM 06/11/2023 17:44.

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 28/04/2015, OBSERVADAS AS CONDICOES LEGAIS