BR112016016342A2 - POLYPEPTIDE VARIANT HAVING LACTASE ACTIVITY, NUCLEIC ACID SEQUENCE, NUCLEIC ACID CONSTRUCT, RECOMBINANT EXPRESSION VECTOR, METHOD FOR PRODUCING A LACTASE POLYPEPTIDE, METHOD FOR PRODUCING A LACTASE POLYPEPTIDE VARIANT, COMPOSITION, USE OF A POLYPEPTIDE VARIANT, PROCESS FOR PRODUCING A POLYPEPTIDE VARIANT PRODUCTION OF A DAIRY PRODUCT AND MILK PRODUCT - Google Patents

POLYPEPTIDE VARIANT HAVING LACTASE ACTIVITY, NUCLEIC ACID SEQUENCE, NUCLEIC ACID CONSTRUCT, RECOMBINANT EXPRESSION VECTOR, METHOD FOR PRODUCING A LACTASE POLYPEPTIDE, METHOD FOR PRODUCING A LACTASE POLYPEPTIDE VARIANT, COMPOSITION, USE OF A POLYPEPTIDE VARIANT, PROCESS FOR PRODUCING A POLYPEPTIDE VARIANT PRODUCTION OF A DAIRY PRODUCT AND MILK PRODUCT

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Publication number
BR112016016342A2
BR112016016342A2 BR112016016342A BR112016016342A BR112016016342A2 BR 112016016342 A2 BR112016016342 A2 BR 112016016342A2 BR 112016016342 A BR112016016342 A BR 112016016342A BR 112016016342 A BR112016016342 A BR 112016016342A BR 112016016342 A2 BR112016016342 A2 BR 112016016342A2
Authority
BR
Brazil
Prior art keywords
lactase
producing
polypeptide variant
nucleic acid
polypeptide
Prior art date
Application number
BR112016016342A
Other languages
Portuguese (pt)
Other versions
BR112016016342B1 (en
Inventor
Marcel De Jong René
Maria MÜLLER Ulrike
Jacobus Theodorus Dekker Petrus
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of BR112016016342A2 publication Critical patent/BR112016016342A2/en
Publication of BR112016016342B1 publication Critical patent/BR112016016342B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01107—Protein-glucosylgalactosylhydroxylysine glucosidase (3.2.1.107)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

VARIANTES DE ENZIMA MELHORADAS. A invenção se refere a um polipeptídeo variante que tem atividade de lactase. A invenção também se refere a uma sequência de ácido nucleico que codifica tal polipeptídeo variante, a um construto de ácido nucleico que compreende a dita sequência de ácido nucleico, a um vetor de expressão recombinante que compreende o dito construto de ácido nucleico e a uma célula hospedeira recombinante que compreende o dito vetor de expressão. Além disso, a invenção se refere a um método para produzir uma variante de lactase por meio do uso de tal célula hospedeira. Ademais, a invenção se refere a um método para produzir uma variante de polipeptídeo de lactase. A invenção se refere, ainda, a uma composição que compreende uma variante de lactase, ao uso de tal variante de lactase ou ao uso de uma composição que contém variante de lactase na preparação de um produto lácteo, a um processo para a produção de um produto lácteo e ao produto lácteo resultante.IMPROVED ENZYME VARIANTS. The invention relates to a variant polypeptide that has lactase activity. The invention also relates to a nucleic acid sequence encoding such a variant polypeptide, a nucleic acid construct comprising said nucleic acid sequence, a recombinant expression vector comprising said nucleic acid construct, and a cell recombinant host comprising said expression vector. Furthermore, the invention relates to a method of producing a lactase variant through the use of such a host cell. Furthermore, the invention relates to a method for producing a lactase polypeptide variant. The invention further relates to a composition comprising a lactase variant, the use of such a lactase variant or the use of a composition containing a lactase variant in the preparation of a dairy product, a process for producing a dairy product and the resulting dairy product.

BR112016016342-7A 2014-01-14 2015-01-13 POLYPEPTIDE VARIANT HAVING LACTASE ACTIVITY, METHOD FOR PRODUCING A POLYPEPTIDE LACTASE VARIANT, COMPOSITION, USE OF A POLYPEPTIDE VARIANT AND PROCESS FOR PRODUCING A DAIRY PRODUCT BR112016016342B1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14151124.6 2014-01-14
EP14151124 2014-01-14
EP14169816 2014-05-26
EP14169816.7 2014-05-26
PCT/EP2015/050503 WO2015107050A1 (en) 2014-01-14 2015-01-13 Improved enzyme variants of lactase from kluyveromyces lactis

Publications (2)

Publication Number Publication Date
BR112016016342A2 true BR112016016342A2 (en) 2017-10-03
BR112016016342B1 BR112016016342B1 (en) 2023-05-09

Family

ID=52350110

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016016342-7A BR112016016342B1 (en) 2014-01-14 2015-01-13 POLYPEPTIDE VARIANT HAVING LACTASE ACTIVITY, METHOD FOR PRODUCING A POLYPEPTIDE LACTASE VARIANT, COMPOSITION, USE OF A POLYPEPTIDE VARIANT AND PROCESS FOR PRODUCING A DAIRY PRODUCT

Country Status (10)

Country Link
US (2) US9994837B2 (en)
EP (2) EP3339433A1 (en)
JP (1) JP6632526B2 (en)
CN (1) CN105899659A (en)
AU (1) AU2015206094B2 (en)
BR (1) BR112016016342B1 (en)
DK (1) DK3094723T3 (en)
ES (1) ES2670422T3 (en)
MX (1) MX2016009149A (en)
WO (1) WO2015107050A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11479763B2 (en) * 2017-04-07 2022-10-25 Dupont Nutrition Biosciences Aps Bacillus host cells producing β-galactosidases and lactases in the absence of p-nitrobenzylesterase side activity
MA50220A (en) * 2017-04-11 2020-07-22 Chr Hansen As LACTASE ENZYMES WITH ENHANCED ACTIVITY AT LOW TEMPERATURES
CA3059275A1 (en) * 2017-04-11 2018-10-18 Chr. Hansen A/S Lactase enzymes with improved properties
AU2018251967B2 (en) * 2017-04-11 2022-04-14 Chr. Hansen A/S Lactase enzymes with improved properties
CN107523595A (en) * 2017-08-21 2017-12-29 天津大学 A kind of environment-friendly preparation method thereof of high-purity oligomate
CN108102936B (en) * 2018-02-26 2020-12-22 董颖军 Kluyveromyces lactis mutant strain, glycosidase thereof and application thereof
AU2019392275A1 (en) * 2018-12-03 2021-06-24 Dupont Nutrition Biosciences Aps Use of lactase to provide high GOS fiber level and low lactose level
CN110616228B (en) * 2019-10-22 2023-07-04 怀化学院 High-activity lactase gene and preparation method and application of recombinant protein thereof
CN110951630B (en) * 2019-12-10 2021-06-18 刘建蔚 Kluyveromyces lactis mutant strain XT82 and application thereof
US20230220366A1 (en) * 2020-02-26 2023-07-13 Novozymes A/S Polypeptide Variants
CN114806906B (en) * 2022-05-31 2023-02-21 江南大学 Kluyveromyces lactis for producing neutral lactase

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5492813A (en) * 1993-11-01 1996-02-20 Boehringer Mannheim Corporation Muteins of β-galactosidase fragments having increased activity
EP1261697B1 (en) * 1999-07-09 2004-07-28 Université de Liège Cold-active beta galactosidase, the process for its preparation and the use thereof
DK1283876T3 (en) * 2000-05-26 2007-02-19 Arla Foods Amba Betagalactosidase isolated from Bifidobacterium
WO2004074468A1 (en) 2002-05-30 2004-09-02 Dsm Ip Assets B.V. Novel pectinases and uses thereof
PL1954808T3 (en) * 2005-11-28 2014-05-30 Dsm Ip Assets Bv Enzyme preparation yielding a clean taste
EP2380988A3 (en) * 2007-07-10 2012-04-11 Mosanto Technology LLC Transgenic plants with enhanced agronomic traits
CN102250856B (en) * 2011-06-23 2013-09-18 江南大学 Construction of heat-resistant beta-galactosidase mutant
ES2397334B1 (en) * 2011-06-24 2014-06-06 Queizúar, S.L. KLUYVEROMYCES LACTIS YEAST CEPA AND PROCEDURE FOR OBTAINING SUGARS, ETHANOL, BETA-GALACTOSIDASE AND BIOMASS.

Also Published As

Publication number Publication date
CN105899659A (en) 2016-08-24
JP2017507644A (en) 2017-03-23
EP3339433A1 (en) 2018-06-27
US10612013B2 (en) 2020-04-07
ES2670422T3 (en) 2018-05-30
EP3094723A1 (en) 2016-11-23
DK3094723T3 (en) 2018-06-06
EP3094723B1 (en) 2018-02-28
US20160333331A1 (en) 2016-11-17
MX2016009149A (en) 2016-09-09
NZ720283A (en) 2022-03-25
WO2015107050A1 (en) 2015-07-23
JP6632526B2 (en) 2020-01-22
AU2015206094B2 (en) 2020-09-10
US20180320156A1 (en) 2018-11-08
US9994837B2 (en) 2018-06-12
BR112016016342B1 (en) 2023-05-09
AU2015206094A1 (en) 2016-06-09

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B350 Update of information on the portal [chapter 15.35 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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