BR112014022117B8 - Célula hospedeira de levedura recombinante para a produção de malonato e métodos de produção de malonato e acrilato - Google Patents
Célula hospedeira de levedura recombinante para a produção de malonato e métodos de produção de malonato e acrilatoInfo
- Publication number
- BR112014022117B8 BR112014022117B8 BR112014022117A BR112014022117A BR112014022117B8 BR 112014022117 B8 BR112014022117 B8 BR 112014022117B8 BR 112014022117 A BR112014022117 A BR 112014022117A BR 112014022117 A BR112014022117 A BR 112014022117A BR 112014022117 B8 BR112014022117 B8 BR 112014022117B8
- Authority
- BR
- Brazil
- Prior art keywords
- malonate
- production
- host cell
- yeast host
- recombinant yeast
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/02—Thioester hydrolases (3.1.2)
- C12Y301/02004—3-Hydroxyisobutyryl-CoA hydrolase (3.1.2.4)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/347—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups
- C07C51/377—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by splitting-off hydrogen or functional groups; by hydrogenolysis of functional groups
- C07C51/38—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by splitting-off hydrogen or functional groups; by hydrogenolysis of functional groups by decarboxylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/08—Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/30—Preparation of carboxylic acid esters by modifying the acid moiety of the ester, such modification not being an introduction of an ester group
- C07C67/31—Preparation of carboxylic acid esters by modifying the acid moiety of the ester, such modification not being an introduction of an ester group by introduction of functional groups containing oxygen only in singly bound form
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261607479P | 2012-03-06 | 2012-03-06 | |
| US61/607.479 | 2012-03-06 | ||
| US201261619112P | 2012-04-02 | 2012-04-02 | |
| US61/619.112 | 2012-04-02 | ||
| PCT/US2013/029441 WO2013134424A1 (en) | 2012-03-06 | 2013-03-06 | Recombinant host cells for the production of malonate |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112014022117A2 BR112014022117A2 (enExample) | 2017-06-20 |
| BR112014022117B1 BR112014022117B1 (pt) | 2022-02-15 |
| BR112014022117B8 true BR112014022117B8 (pt) | 2023-01-10 |
Family
ID=49117310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112014022117A BR112014022117B8 (pt) | 2012-03-06 | 2013-03-06 | Célula hospedeira de levedura recombinante para a produção de malonato e métodos de produção de malonato e acrilato |
Country Status (8)
| Country | Link |
|---|---|
| US (5) | US9816114B2 (enExample) |
| EP (2) | EP2823032B1 (enExample) |
| CN (4) | CN107586732A (enExample) |
| AU (2) | AU2013230930B2 (enExample) |
| BR (1) | BR112014022117B8 (enExample) |
| ES (1) | ES2698500T3 (enExample) |
| MY (1) | MY173953A (enExample) |
| WO (1) | WO2013134424A1 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2823032B1 (en) | 2012-03-06 | 2018-08-22 | Lygos, Inc. | Recombinant host cells for the production of malonate |
| EP3161142A4 (en) * | 2014-06-26 | 2018-05-16 | Lygos, Inc. | Recombinant host cells for the production of malonate |
| FR3030575A1 (fr) * | 2014-12-22 | 2016-06-24 | Inst Nat Des Sciences Appliquees De Toulouse | Procede de synthese d'acides gras |
| WO2016130597A1 (en) * | 2015-02-10 | 2016-08-18 | GeneSys Consulting, LLC | Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same |
| US20160230198A1 (en) * | 2015-02-10 | 2016-08-11 | GeneSys Consulting, LLC | Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same |
| CA3042854A1 (en) * | 2015-11-12 | 2017-05-18 | Lygos, Inc. | Recombinant host cells and methods for the anaerobic production of l-aspartate and beta-alanine |
| KR101828551B1 (ko) * | 2016-01-11 | 2018-02-13 | 한국과학기술원 | 말론산 생성능을 가지는 재조합 변이미생물 및 이를 이용한 말론산의 제조방법 |
| US11326191B2 (en) * | 2016-11-14 | 2022-05-10 | Lygos, Inc. | Process for purification of malonic acid from fermentation broth |
| WO2019040737A1 (en) * | 2017-08-23 | 2019-02-28 | Lygos, Inc. | RECOVERING MALONIC ACID AND ITS ESTERS |
| CN108315343A (zh) * | 2018-02-11 | 2018-07-24 | 楚雄医药高等专科学校 | 一种产木犀草素酵母菌株的基因合成方法及菌株和应用 |
| WO2019160862A1 (en) * | 2018-02-13 | 2019-08-22 | Lygos, Inc. | Method for preparation of diester derivatives of malonic acid |
| EP3775182A1 (en) * | 2018-03-30 | 2021-02-17 | INVISTA Textiles (U.K.) Limited | Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides |
| EP3810786A4 (en) * | 2018-06-25 | 2022-06-08 | Lygos, Inc. | RECOMBINED HOST CELLS AND METHODS FOR PRODUCING ASPARTIC ACID AND BETA-ALANINE |
| US20220049279A1 (en) * | 2018-12-18 | 2022-02-17 | Alderys | Malonic semi-aldehyde-producing yeasts |
| CN111334444A (zh) * | 2018-12-19 | 2020-06-26 | 中国科学院微生物研究所 | 长链二元酸生产菌株及其制备方法和应用 |
| CN110499259B (zh) * | 2019-07-22 | 2021-07-27 | 浙江工业大学 | 一种解酯耶氏酵母yw100-1及其应用 |
| CN112410389B (zh) * | 2019-08-23 | 2023-07-18 | 中国科学院微生物研究所 | 支链α-酮酸脱氢酶复合体在制备丙二酸单酰辅酶A中的应用 |
| CN119709450A (zh) * | 2019-08-30 | 2025-03-28 | 利戈斯股份有限公司 | 用于生产丙二酸的重组宿主细胞 |
| US20230203430A1 (en) * | 2019-12-23 | 2023-06-29 | Cargill, Incorporated | Fermentation pathway for producing malonic acid |
| EP4158046A4 (en) * | 2020-05-29 | 2025-07-16 | Cargill Inc | PROCESS FOR RECOVERING MALONATE FROM FERMENTATION BROTH |
| WO2022061230A2 (en) * | 2020-09-21 | 2022-03-24 | Lygos, Inc. | Continuous ion exchange and esterification of fermented malonic acid |
| US20250290082A1 (en) * | 2021-05-28 | 2025-09-18 | Cargill, Incorporated | Fermentation method for producing malonic acid |
| WO2022265934A1 (en) * | 2021-06-17 | 2022-12-22 | Lygos, Inc. | Preparing diesters of malonic acid |
| CN116103172B (zh) * | 2021-11-11 | 2025-06-03 | 江南大学 | 一种酿酒酵母通过丙氨酸途径生产丙二酸的方法 |
| CN116103173B (zh) * | 2021-11-11 | 2025-06-03 | 江南大学 | 一种提高酿酒酵母生产丙二酸的方法 |
| CN116135981B (zh) * | 2021-11-18 | 2024-07-12 | 中国科学院天津工业生物技术研究所 | 一种合成丙二酸的方法及应用 |
| CN114736841B (zh) * | 2022-04-02 | 2023-12-19 | 万华化学集团股份有限公司 | 一种重组大肠杆菌及其制备方法与应用 |
| WO2024054828A2 (en) * | 2022-09-06 | 2024-03-14 | Lygos, Inc. | Recombinant host cells for the production of malonate |
| EP4585586A4 (en) * | 2022-09-09 | 2025-12-03 | Xiamen Sinopeg Biotech Co Ltd | CARBON CORE CATIONIC LIPID |
| WO2025038447A1 (en) * | 2023-08-11 | 2025-02-20 | Ginkgo Bioworks, Inc. | Engineered cells for production of malonate |
| CN117757766B (zh) * | 2024-02-20 | 2024-05-14 | 中国科学院天津工业生物技术研究所 | 醛还原酶突变体及其在合成d-1,2,4-丁三醇中的应用 |
| CN119391682A (zh) * | 2025-01-03 | 2025-02-07 | 浙江科技大学 | 一种谷氨酸脱羧酶多位点组合突变体及其应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2205753A1 (en) * | 1996-05-22 | 1997-11-22 | Kei Aoki | Method for preparing malonate group-containing acrylate monomers |
| BR9704357A (pt) * | 1997-08-13 | 1999-05-11 | Trikem Sa | Processo para produção de acido malônico e de ésteres de acido malônico |
| DE19752041A1 (de) | 1997-11-24 | 1999-05-27 | Huels Chemische Werke Ag | Verfahren zur C-Alkylierung von Malonsäureestern |
| CA2861834A1 (en) * | 2004-07-16 | 2006-01-26 | Technische Universiteit Delft | Metabolic engineering of xylose fermenting eukaryotic cells |
| CN101283089A (zh) * | 2005-10-07 | 2008-10-08 | 加利福尼亚大学董事会 | 修饰细胞色素p450酶的编码核酸和其应用方法 |
| WO2008143679A2 (en) * | 2006-06-01 | 2008-11-27 | Verenium Corporation | Nucleic acids and proteins and methods for making and using them |
| KR20120068763A (ko) * | 2009-04-30 | 2012-06-27 | 게노마티카 인코포레이티드 | 아이소프로판올, ｎ-부탄올 및 아이소부탄올 생산 유기체 |
| MX2012003025A (es) * | 2009-09-09 | 2012-06-27 | Genomatica Inc | Microorganismos y metodos para la co-produccion de isopropanol con alcoholes, dioles y acidos primarios. |
| GB201002824D0 (en) * | 2010-02-19 | 2010-04-07 | Temasek Polytechnic | A method of preparing a substrate for immobilization of functional substances thereon and the substrate obtained therefrom |
| EP3287519B1 (en) * | 2010-11-22 | 2020-03-18 | Cargill, Incorporated | Compositions and methods for 3-hydroxypropionic acid production |
| EP2823032B1 (en) | 2012-03-06 | 2018-08-22 | Lygos, Inc. | Recombinant host cells for the production of malonate |
-
2013
- 2013-03-06 EP EP13758501.4A patent/EP2823032B1/en active Active
- 2013-03-06 AU AU2013230930A patent/AU2013230930B2/en not_active Ceased
- 2013-03-06 ES ES13758501T patent/ES2698500T3/es active Active
- 2013-03-06 CN CN201710980850.6A patent/CN107586732A/zh active Pending
- 2013-03-06 CN CN201380023454.4A patent/CN104284974B/zh active Active
- 2013-03-06 BR BR112014022117A patent/BR112014022117B8/pt active IP Right Grant
- 2013-03-06 CN CN202111260243.5A patent/CN114395496A/zh active Pending
- 2013-03-06 US US14/386,272 patent/US9816114B2/en not_active Expired - Fee Related
- 2013-03-06 WO PCT/US2013/029441 patent/WO2013134424A1/en not_active Ceased
- 2013-03-06 EP EP18182411.1A patent/EP3404094A1/en active Pending
- 2013-03-06 CN CN202410200340.2A patent/CN118086086A/zh active Pending
- 2013-03-06 MY MYPI2014002575A patent/MY173953A/en unknown
-
2017
- 2017-06-19 US US15/627,191 patent/US10472653B2/en active Active
-
2019
- 2019-01-10 AU AU2019200158A patent/AU2019200158A1/en not_active Abandoned
- 2019-10-07 US US16/595,253 patent/US11248215B2/en active Active
-
2021
- 2021-12-29 US US17/646,437 patent/US20220119783A1/en not_active Abandoned
-
2023
- 2023-03-20 US US18/123,840 patent/US20240191216A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20160177345A1 (en) | 2016-06-23 |
| BR112014022117A2 (enExample) | 2017-06-20 |
| CN118086086A (zh) | 2024-05-28 |
| US20170362614A1 (en) | 2017-12-21 |
| US9816114B2 (en) | 2017-11-14 |
| CN104284974B (zh) | 2018-06-26 |
| EP3404094A1 (en) | 2018-11-21 |
| CN104284974A (zh) | 2015-01-14 |
| US20200071733A1 (en) | 2020-03-05 |
| US11248215B2 (en) | 2022-02-15 |
| AU2013230930B2 (en) | 2018-10-11 |
| WO2013134424A1 (en) | 2013-09-12 |
| AU2013230930A1 (en) | 2014-10-23 |
| CN107586732A (zh) | 2018-01-16 |
| US20220119783A1 (en) | 2022-04-21 |
| BR112014022117B1 (pt) | 2022-02-15 |
| MY173953A (en) | 2020-02-28 |
| ES2698500T3 (es) | 2019-02-05 |
| EP2823032B1 (en) | 2018-08-22 |
| AU2019200158A1 (en) | 2019-01-31 |
| CN114395496A (zh) | 2022-04-26 |
| EP2823032A4 (en) | 2016-03-02 |
| US10472653B2 (en) | 2019-11-12 |
| EP2823032A1 (en) | 2015-01-14 |
| US20240191216A1 (en) | 2024-06-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 06/03/2013, OBSERVADAS AS CONDICOES LEGAIS. |
|
| B16C | Correction of notification of the grant [chapter 16.3 patent gazette] |
Free format text: REF. RPI 2667 DE 15/02/2022 QUANTO AO ENDERECO. |