AU2013230930B2 - Recombinant host cells for the production of malonate - Google Patents
Recombinant host cells for the production of malonate Download PDFInfo
- Publication number
- AU2013230930B2 AU2013230930B2 AU2013230930A AU2013230930A AU2013230930B2 AU 2013230930 B2 AU2013230930 B2 AU 2013230930B2 AU 2013230930 A AU2013230930 A AU 2013230930A AU 2013230930 A AU2013230930 A AU 2013230930A AU 2013230930 B2 AU2013230930 B2 AU 2013230930B2
- Authority
- AU
- Australia
- Prior art keywords
- malonate
- seq
- coa
- malonyl
- hydrolase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
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- 238000000034 method Methods 0.000 claims abstract description 148
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- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 80
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/347—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups
- C07C51/377—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by splitting-off hydrogen or functional groups; by hydrogenolysis of functional groups
- C07C51/38—Preparation of carboxylic acids or their salts, halides or anhydrides by reactions not involving formation of carboxyl groups by splitting-off hydrogen or functional groups; by hydrogenolysis of functional groups by decarboxylation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/08—Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/30—Preparation of carboxylic acid esters by modifying the acid moiety of the ester, such modification not being an introduction of an ester group
- C07C67/31—Preparation of carboxylic acid esters by modifying the acid moiety of the ester, such modification not being an introduction of an ester group by introduction of functional groups containing oxygen only in singly bound form
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- Botany (AREA)
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| ES2698500T3 (es) | 2012-03-06 | 2019-02-05 | Lygos Inc | Células huésped recombinantes para la producción de malonato |
| EP3161142A4 (en) * | 2014-06-26 | 2018-05-16 | Lygos, Inc. | Recombinant host cells for the production of malonate |
| FR3030575A1 (fr) * | 2014-12-22 | 2016-06-24 | Inst Nat Des Sciences Appliquees De Toulouse | Procede de synthese d'acides gras |
| WO2016130597A1 (en) * | 2015-02-10 | 2016-08-18 | GeneSys Consulting, LLC | Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same |
| US20160230198A1 (en) * | 2015-02-10 | 2016-08-11 | GeneSys Consulting, LLC | Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same |
| CN109072172A (zh) * | 2015-11-12 | 2018-12-21 | 利戈斯股份有限公司 | 用于厌氧产生L-天冬氨酸和β-丙氨酸的重组宿主细胞和方法 |
| KR101828551B1 (ko) * | 2016-01-11 | 2018-02-13 | 한국과학기술원 | 말론산 생성능을 가지는 재조합 변이미생물 및 이를 이용한 말론산의 제조방법 |
| US11326191B2 (en) | 2016-11-14 | 2022-05-10 | Lygos, Inc. | Process for purification of malonic acid from fermentation broth |
| WO2019040737A1 (en) * | 2017-08-23 | 2019-02-28 | Lygos, Inc. | RECOVERING MALONIC ACID AND ITS ESTERS |
| CN108315343A (zh) * | 2018-02-11 | 2018-07-24 | 楚雄医药高等专科学校 | 一种产木犀草素酵母菌株的基因合成方法及菌株和应用 |
| WO2019160862A1 (en) * | 2018-02-13 | 2019-08-22 | Lygos, Inc. | Method for preparation of diester derivatives of malonic acid |
| EP3775182A1 (en) * | 2018-03-30 | 2021-02-17 | INVISTA Textiles (U.K.) Limited | Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides |
| US20220119821A1 (en) * | 2018-06-25 | 2022-04-21 | Lygos, Inc. | Recombinant host cells and methods for the production of aspartic acid and b-alanine |
| US20220049279A1 (en) * | 2018-12-18 | 2022-02-17 | Alderys | Malonic semi-aldehyde-producing yeasts |
| CN111334444A (zh) * | 2018-12-19 | 2020-06-26 | 中国科学院微生物研究所 | 长链二元酸生产菌株及其制备方法和应用 |
| CN110499259B (zh) * | 2019-07-22 | 2021-07-27 | 浙江工业大学 | 一种解酯耶氏酵母yw100-1及其应用 |
| CN112410389B (zh) * | 2019-08-23 | 2023-07-18 | 中国科学院微生物研究所 | 支链α-酮酸脱氢酶复合体在制备丙二酸单酰辅酶A中的应用 |
| CA3149454A1 (en) * | 2019-08-30 | 2021-03-04 | Lygos, Inc. | Recombinant host cells for the production of malonate |
| WO2021133722A1 (en) * | 2019-12-23 | 2021-07-01 | Cargill, Incorporated | Fermentation pathway for producing malonic acid |
| US20230348353A1 (en) * | 2020-05-29 | 2023-11-02 | Cargill, Incorporated | Method for recovering malonate from a fermentation broth |
| EP4214326A4 (en) * | 2020-09-21 | 2024-11-06 | Lygos, Inc. | CONTINUOUS ION EXCHANGE AND ESTERIFICATION OF FERMENTED MALONIC ACID |
| WO2022250697A1 (en) * | 2021-05-28 | 2022-12-01 | Cargill, Incorporated | Fermentation method for producing malonic acid |
| EP4355892A1 (en) * | 2021-06-17 | 2024-04-24 | Lygos, Inc. | Preparing diesters of malonic acid |
| CN116103173B (zh) * | 2021-11-11 | 2025-06-03 | 江南大学 | 一种提高酿酒酵母生产丙二酸的方法 |
| CN116103172B (zh) * | 2021-11-11 | 2025-06-03 | 江南大学 | 一种酿酒酵母通过丙氨酸途径生产丙二酸的方法 |
| CN116135981B (zh) * | 2021-11-18 | 2024-07-12 | 中国科学院天津工业生物技术研究所 | 一种合成丙二酸的方法及应用 |
| CN114736841B (zh) * | 2022-04-02 | 2023-12-19 | 万华化学集团股份有限公司 | 一种重组大肠杆菌及其制备方法与应用 |
| WO2024054828A2 (en) * | 2022-09-06 | 2024-03-14 | Lygos, Inc. | Recombinant host cells for the production of malonate |
| WO2024051825A1 (zh) * | 2022-09-09 | 2024-03-14 | 厦门赛诺邦格生物科技股份有限公司 | 一种碳核阳离子脂质 |
| WO2025038447A1 (en) * | 2023-08-11 | 2025-02-20 | Ginkgo Bioworks, Inc. | Engineered cells for production of malonate |
| CN117757766B (zh) * | 2024-02-20 | 2024-05-14 | 中国科学院天津工业生物技术研究所 | 醛还原酶突变体及其在合成d-1,2,4-丁三醇中的应用 |
| CN119391682A (zh) * | 2025-01-03 | 2025-02-07 | 浙江科技大学 | 一种谷氨酸脱羧酶多位点组合突变体及其应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0808822B1 (en) * | 1996-05-22 | 2001-10-04 | Nippon Paint Co., Ltd. | Method for preparing malonate group-containing acrylate monomers |
| BR9704357A (pt) * | 1997-08-13 | 1999-05-11 | Trikem Sa | Processo para produção de acido malônico e de ésteres de acido malônico |
| DE19752041A1 (de) | 1997-11-24 | 1999-05-27 | Huels Chemische Werke Ag | Verfahren zur C-Alkylierung von Malonsäureestern |
| US8034591B2 (en) * | 2004-07-16 | 2011-10-11 | Techische Universiteit Delft | Xylose-fermenting yeast engineered to increase flux of the pentose phosphate pathway |
| ZA200803368B (en) * | 2005-10-07 | 2009-09-30 | Univ California | Nucleic acids encoding modified cytochrome P450 enzymes and methods of use thereof |
| WO2008143679A2 (en) * | 2006-06-01 | 2008-11-27 | Verenium Corporation | Nucleic acids and proteins and methods for making and using them |
| BRPI1013505A2 (pt) | 2009-04-30 | 2018-02-14 | Genomatica Inc | organismos para a produção de isopropanol, n-butanol, e isobutanol |
| SG179048A1 (en) * | 2009-09-09 | 2012-04-27 | Genomatica Inc | Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids |
| GB201002824D0 (en) * | 2010-02-19 | 2010-04-07 | Temasek Polytechnic | A method of preparing a substrate for immobilization of functional substances thereon and the substrate obtained therefrom |
| CN103502432A (zh) * | 2010-11-22 | 2014-01-08 | 诺维信股份有限公司 | 用于生产3-羟基丙酸的组合物和方法 |
| ES2698500T3 (es) | 2012-03-06 | 2019-02-05 | Lygos Inc | Células huésped recombinantes para la producción de malonato |
-
2013
- 2013-03-06 ES ES13758501T patent/ES2698500T3/es active Active
- 2013-03-06 WO PCT/US2013/029441 patent/WO2013134424A1/en not_active Ceased
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- 2013-03-06 EP EP13758501.4A patent/EP2823032B1/en active Active
- 2013-03-06 CN CN202111260243.5A patent/CN114395496A/zh active Pending
- 2013-03-06 EP EP18182411.1A patent/EP3404094A1/en active Pending
- 2013-03-06 CN CN202410200340.2A patent/CN118086086A/zh active Pending
- 2013-03-06 CN CN201380023454.4A patent/CN104284974B/zh active Active
- 2013-03-06 US US14/386,272 patent/US9816114B2/en not_active Expired - Fee Related
- 2013-03-06 CN CN201710980850.6A patent/CN107586732A/zh active Pending
- 2013-03-06 MY MYPI2014002575A patent/MY173953A/en unknown
- 2013-03-06 BR BR112014022117A patent/BR112014022117B8/pt active IP Right Grant
-
2017
- 2017-06-19 US US15/627,191 patent/US10472653B2/en active Active
-
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- 2019-01-10 AU AU2019200158A patent/AU2019200158A1/en not_active Abandoned
- 2019-10-07 US US16/595,253 patent/US11248215B2/en active Active
-
2021
- 2021-12-29 US US17/646,437 patent/US20220119783A1/en not_active Abandoned
-
2023
- 2023-03-20 US US18/123,840 patent/US20240191216A1/en not_active Abandoned
Non-Patent Citations (3)
| Title |
|---|
| Claxton HB et al, "Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway", Journal of Biological Chemistry, 2009, 284(8):5021-5029 * |
| Rangan VS et al, Journal of Biological Chemistry, 1997, 272(18):11975-11978 * |
| Rouhier MF, "Characterization of YDR036C From Saccharomyces cerevisiae", Dissertation, Miami University, Miami University and OhioLINK (2011) <URL: https://etd.ohiolink.edu/pg_10?0::NO:10:P10_ETD_SUBID:57666 > * |
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| BR112014022117B1 (pt) | 2022-02-15 |
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| US20220119783A1 (en) | 2022-04-21 |
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| US10472653B2 (en) | 2019-11-12 |
| US20200071733A1 (en) | 2020-03-05 |
| AU2013230930A1 (en) | 2014-10-23 |
| US20170362614A1 (en) | 2017-12-21 |
| CN104284974A (zh) | 2015-01-14 |
| US11248215B2 (en) | 2022-02-15 |
| AU2019200158A1 (en) | 2019-01-31 |
| US9816114B2 (en) | 2017-11-14 |
| US20160177345A1 (en) | 2016-06-23 |
| ES2698500T3 (es) | 2019-02-05 |
| BR112014022117B8 (pt) | 2023-01-10 |
| CN114395496A (zh) | 2022-04-26 |
| EP2823032A1 (en) | 2015-01-14 |
| CN107586732A (zh) | 2018-01-16 |
| CN118086086A (zh) | 2024-05-28 |
| BR112014022117A2 (enExample) | 2017-06-20 |
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