BR112013009244A2 - processo para a produção semi-sintética de vetores de dna de "minicírculo" altamente puros, a partir de plasmídios - Google Patents

processo para a produção semi-sintética de vetores de dna de "minicírculo" altamente puros, a partir de plasmídios

Info

Publication number
BR112013009244A2
BR112013009244A2 BR112013009244A BR112013009244A BR112013009244A2 BR 112013009244 A2 BR112013009244 A2 BR 112013009244A2 BR 112013009244 A BR112013009244 A BR 112013009244A BR 112013009244 A BR112013009244 A BR 112013009244A BR 112013009244 A2 BR112013009244 A2 BR 112013009244A2
Authority
BR
Brazil
Prior art keywords
highly pure
plasmids
mini
semi
circle
Prior art date
Application number
BR112013009244A
Other languages
English (en)
Inventor
Bernd Rehberger
Class Wodarczyk
Markus Heine
Roland Wagner
Original Assignee
Rentschler Biotech Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rentschler Biotech Gmbh filed Critical Rentschler Biotech Gmbh
Publication of BR112013009244A2 publication Critical patent/BR112013009244A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

processo para a produção semi-sintética de vetores de dna de "minicírculo" altamente puros, a partir de plasmídios. a invenção refere-se a processos e reagentes para a produção de vetores de dna, em particular, vetores de minicírculo (mc) de dna em forma super-helicoidal. além disso, a invenção refere-se a preparações altamente puras de vetores de dna circulares, em particular, vetores dna mc.
BR112013009244A 2010-10-05 2011-10-04 processo para a produção semi-sintética de vetores de dna de "minicírculo" altamente puros, a partir de plasmídios BR112013009244A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10186568A EP2439276A1 (de) 2010-10-05 2010-10-05 Verfahren zur semi-synthetischen Herstellung hochreiner "MiniCircle" DNA-Vektoren aus Plasmiden
PCT/EP2011/067280 WO2012045722A1 (de) 2010-10-05 2011-10-04 Verfahren zur semi-synthetischen herstellung hochreiner "minicircle" dna-vektoren aus plasmiden

Publications (1)

Publication Number Publication Date
BR112013009244A2 true BR112013009244A2 (pt) 2016-07-26

Family

ID=43733185

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013009244A BR112013009244A2 (pt) 2010-10-05 2011-10-04 processo para a produção semi-sintética de vetores de dna de "minicírculo" altamente puros, a partir de plasmídios

Country Status (8)

Country Link
US (1) US20130203121A1 (pt)
EP (2) EP2439276A1 (pt)
AU (1) AU2011311637A1 (pt)
BR (1) BR112013009244A2 (pt)
CA (1) CA2813664A1 (pt)
IL (1) IL225518A0 (pt)
SG (1) SG189272A1 (pt)
WO (1) WO2012045722A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201307075D0 (en) 2013-04-19 2013-05-29 Mayrhofer Peter Plasmid for minicircle production
PT107663B (pt) * 2014-05-28 2019-02-26 Inst Superior Tecnico Processo para a purificação de minicírculos
CN115066255A (zh) * 2019-09-18 2022-09-16 星际治疗有限公司 合成的dna载体和使用方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0108968D0 (en) 2001-04-10 2001-05-30 Imp College Innovations Ltd Methods
US20040191799A1 (en) * 2003-03-25 2004-09-30 Hyman Edward David Method for plasmid preparation by conversion of open circular plasmid
US7510856B2 (en) * 2003-03-25 2009-03-31 Hyman Edward D Method for plasmid preparation by conversion of open circular plasmid to supercoiled plasmid
AT412400B (de) 2003-05-08 2005-02-25 Mayrhofer Peter Mag Dr Minicircle-herstellung
US7622252B2 (en) 2005-06-10 2009-11-24 Baylor College Of Medicine Generation of minicircle DNA with physiological supercoiling
CN106434725B (zh) 2008-07-03 2019-08-20 小利兰·斯坦福大学托管委员会 小环dna载体制剂及其制备方法和使用方法

Also Published As

Publication number Publication date
US20130203121A1 (en) 2013-08-08
WO2012045722A1 (de) 2012-04-12
CA2813664A1 (en) 2012-04-12
EP2625275A1 (de) 2013-08-14
SG189272A1 (en) 2013-05-31
EP2439276A1 (de) 2012-04-11
AU2011311637A1 (en) 2013-04-18
IL225518A0 (en) 2013-06-27
AU2011311637A8 (en) 2013-05-02

Similar Documents

Publication Publication Date Title
PH12015501672A1 (en) Binding molecules for bcma and cd3
PH12014501091A1 (en) Binding molecules for bcma and cd3
SI3536703T1 (sl) Konsenzni prostatični antigeni, molekule nukleinske kisline, ki jih kodirajo in uporabe le-teh
MY171038A (en) Bispecific antigen binding molecules
WO2014191359A9 (en) Aptamers and use of the aptamers in the diagnosis and treatment of cancer
EP2709970A4 (en) INTEGRATED PROCESS FOR THE PREPARATION OF 1-CHLORO-3,3,3-TRIFLUORPROPENES
EP2536675A4 (en) INTEGRATED PROCESS AND METHODS OF PRODUCING (E) -1-CHLORO-3,3,3-TRIFLUOROPROPENE
WO2013021279A3 (en) Highly galactosylated antibodies
IT1405680B1 (it) Processo per la produzione di l-fucosio.
PH12016501094A1 (en) Recombinant microorganism for improved production of fine chemicals
MX2016003737A (es) Microorganismo recombinante para la mejora de produccion de productos quimicos finos.
EP2677054A4 (en) DUPLEX STAINLESS STEEL AND PROCESS FOR PRODUCTION THEREOF
EP2738253A4 (en) CELL FOR USE IN IMMUNOTHERAPY WITH A MODIFIED NUCLEIC ACIDIC CONSTRUCTIVE FOR WILMS TUMORGEN PRODUCT OR FRAGMENT THEREOF, METHOD FOR PRODUCING THIS CELL AND NUCLEIC ACIDIC CONCRETE SAID
EP2888369A4 (en) MICRO-ORGANISMS AND METHODS FOR IMPROVING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL AND FOR THE PREPARATION OF 1,4-BANDALIUM
WO2014017493A9 (ja) ワクチン
IL214621A (en) An expression vector system containing two selection markers, a host cell and a production process containing it, and a method for making or selecting this host cell
IN2015DN01811A (pt)
EP2826862A4 (en) SYNTHESIS METHOD OF GENES, DNA CHIP AND KIT
WO2013053857A3 (en) Gene cluster for biosynthesis of griselimycin and methylgriselimycin
HK1199242A1 (zh) 生產 -丁二烯的方法
HUP1000366A2 (en) Novel proteins, their production process and use tereof
BR112013009244A2 (pt) processo para a produção semi-sintética de vetores de dna de "minicírculo" altamente puros, a partir de plasmídios
MX2015007775A (es) Polipeptidos que poseen actividad proteasa y polinucleotidos que los codifican.
PT2861673T (pt) Corantes reactivos sem metal, processo para a sua produção e sua utilização
PT2294044T (pt) Fluoroalquiloxi-alcanos, processo para a sua produção e suas utilizações

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 3A, 4A E 5A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2384 DE 13-09-2016 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.

B350 Update of information on the portal [chapter 15.35 patent gazette]