BR112012003239A2 - microorganismos desidratados revestidos com estabilidade e viabilidade melhoradas - Google Patents

microorganismos desidratados revestidos com estabilidade e viabilidade melhoradas

Info

Publication number
BR112012003239A2
BR112012003239A2 BR112012003239A BR112012003239A BR112012003239A2 BR 112012003239 A2 BR112012003239 A2 BR 112012003239A2 BR 112012003239 A BR112012003239 A BR 112012003239A BR 112012003239 A BR112012003239 A BR 112012003239A BR 112012003239 A2 BR112012003239 A2 BR 112012003239A2
Authority
BR
Brazil
Prior art keywords
viability
improved stability
coated dehydrated
dehydrated microorganisms
microorganisms
Prior art date
Application number
BR112012003239A
Other languages
English (en)
Other versions
BR112012003239B1 (pt
BR112012003239A8 (pt
Inventor
Berger Claudette
Henri Erwan
Babin Geoffrey
Hedegaard Jensen Hans
Mazeaud Isabelle
Philippe Obert Jean-
Tse Kathryn
Chaigneau Patrick
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco filed Critical Danisco
Publication of BR112012003239A2 publication Critical patent/BR112012003239A2/pt
Publication of BR112012003239A8 publication Critical patent/BR112012003239A8/pt
Publication of BR112012003239B1 publication Critical patent/BR112012003239B1/pt

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065Microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • A23P10/30Encapsulation of particles, e.g. foodstuff additives
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/30Layered or coated, e.g. dust-preventing coatings
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/30Layered or coated, e.g. dust-preventing coatings
    • C05G5/36Layered or coated, e.g. dust-preventing coatings layered or coated with sulfur
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/30Layered or coated, e.g. dust-preventing coatings
    • C05G5/37Layered or coated, e.g. dust-preventing coatings layered or coated with a polymer
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08KUse of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K3/00Use of inorganic substances as compounding ingredients
    • C08K3/32Phosphorus-containing compounds
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08KUse of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K3/00Use of inorganic substances as compounding ingredients
    • C08K3/34Silicon-containing compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09DCOATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D101/00Coating compositions based on cellulose, modified cellulose, or cellulose derivatives
    • C09D101/08Cellulose derivatives
    • C09D101/26Cellulose ethers
    • C09D101/28Alkyl ethers
    • C09D101/284Alkyl ethers with hydroxylated hydrocarbon radicals
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09DCOATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D105/00Coating compositions based on polysaccharides or on their derivatives, not provided for in groups C09D101/00 or C09D103/00
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04Preserving or maintaining viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08KUse of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K3/00Use of inorganic substances as compounding ingredients
    • C08K3/32Phosphorus-containing compounds
    • C08K2003/321Phosphates
    • C08K2003/324Alkali metal phosphate

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials Engineering (AREA)
  • Nutrition Science (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Meat, Egg Or Seafood Products (AREA)
BR112012003239-9A 2009-08-14 2010-08-13 microrganismos desidratados revestidos com estabilidade e viabilidade melhoradas BR112012003239B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US23389909P 2009-08-14 2009-08-14
US61/233,899 2009-08-14
US33191810P 2010-05-06 2010-05-06
US61/331,918 2010-05-06
PCT/EP2010/061819 WO2011018509A1 (en) 2009-08-14 2010-08-13 Coated dehydrated microorganisms with enhanced stability and viability

Publications (3)

Publication Number Publication Date
BR112012003239A2 true BR112012003239A2 (pt) 2017-06-06
BR112012003239A8 BR112012003239A8 (pt) 2018-02-06
BR112012003239B1 BR112012003239B1 (pt) 2021-03-16

Family

ID=42938630

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012003239-9A BR112012003239B1 (pt) 2009-08-14 2010-08-13 microrganismos desidratados revestidos com estabilidade e viabilidade melhoradas

Country Status (9)

Country Link
US (2) US8834853B2 (pt)
EP (1) EP2464721B1 (pt)
CN (2) CN107087706A (pt)
BR (1) BR112012003239B1 (pt)
CA (1) CA2770168C (pt)
DK (1) DK2464721T3 (pt)
MX (1) MX2012001765A (pt)
NZ (1) NZ597968A (pt)
WO (1) WO2011018509A1 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2012001765A (es) * 2009-08-14 2012-03-07 Danisco Microorganismos deshidratados recubiertos con estabilidad y viabilidad mejoradas.
US10370636B2 (en) 2010-12-10 2019-08-06 Dsm Ip Assets B.V. Starter culture compositions
US9334507B2 (en) 2012-06-15 2016-05-10 Microvi Biotech, Inc. Bioprocesses for making butanol
US9255281B2 (en) 2012-06-15 2016-02-09 Microvi Biotech Inc. Bioconversion processes using water-insoluble liquids
US9752164B2 (en) 2012-06-15 2017-09-05 Microvi Biotech, Inc. Enhanced efficiency ethanol and sugar conversion processes
US9212358B2 (en) 2012-06-15 2015-12-15 Microvi Biotech, Inc. Biocatalyst compositions and processes for their use
HUE029766T2 (en) * 2012-08-01 2017-04-28 Purac Biochem Bv Preparation of powdered vinegar
EP2887820B1 (en) * 2012-08-24 2016-11-16 Boehringer Ingelheim Vetmedica GmbH Potassium containing preparation, process for the production thereof and use thereof
WO2018060475A1 (en) 2016-09-29 2018-04-05 Novozymes A/S Spore containing granule
CN110214177A (zh) * 2017-01-19 2019-09-06 杜邦营养生物科学有限公司 具有赋形剂的经干燥的微生物
WO2018134135A1 (en) * 2017-01-19 2018-07-26 Dupont Nutrition Biosciences Aps Dried microorganism with excipient
CN110475479A (zh) 2017-01-31 2019-11-19 堪萨斯州立大学研究基金会 微生物细胞、产生其的方法以及其用途
CN111315241B (zh) * 2017-10-05 2022-04-26 普拉克生化公司 乳酸盐粉末及其制备方法
CA3079562A1 (en) 2017-10-20 2019-04-25 Ms Biotech, Inc. Methods of producing ensiled plant materials using megasphaera elsdenii
BR112020008471B1 (pt) * 2017-11-07 2024-01-23 Purac Biochem B.V. Produto particulado, método para preparar um gênero alimentício ou uma bebida e processo para produzir um produto particulado
CO2020006220A1 (es) 2020-05-21 2020-11-30 Bialtec Sas Formulaciones de aditivos funcionales y su proceso de encapsulación
EP4225031A1 (en) * 2020-10-06 2023-08-16 Danisco US Inc. Readily dispersible shelf-stable bioactive granules

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
US4956295A (en) * 1984-05-21 1990-09-11 Chr. Hansen's Laboratory, Inc. Stabilization of dried bacteria extended in particulate carriers
DE3432394C1 (de) 1984-09-04 1986-01-30 Karl-Heinz 4440 Rheine Brinker Verfahren zur Herstellung eines Hefegranulates mit verzögertem Gäreinsatz
US5275943A (en) * 1991-04-12 1994-01-04 Dituro John W Timed-release tablets for biological degradation of organic matter
AUPM823094A0 (en) 1994-09-16 1994-10-13 Goodman Fielder Limited Probiotic compositions
KR100387245B1 (ko) 1997-10-17 2003-08-19 일양약품주식회사 유산균의안정화를위한미세장용성코팅과립
FR2806417B1 (fr) 2000-03-16 2003-12-26 Lallemand Sa Particules enrobees contenant des microorganismes vivants, procede de production et application desdites particules dans les compositions pharmaceutiques, dietetiques ou alimentaires
US6653062B1 (en) 2000-07-26 2003-11-25 Wisconsin Alumni Research Foundation Preservation and storage medium for biological materials
FR2829147B1 (fr) 2001-08-30 2003-12-12 Agronomique Inst Nat Rech Procede de preparation d'une composition lyophilisee contenant des bacteries lactiques a viabilite et activite bacteriennes ameliorees lors d'un stockage a temperature ambiante et composition obtenue
EP1514553B1 (en) 2003-09-05 2008-02-27 Chung, Myung-jun Lactic acid bacteria powder double-coated using protein and polysaccharide and method preparing the same and a dosage form thereof
DE102004026706A1 (de) 2004-05-28 2005-12-15 Merck Patent Gmbh Orale Darreichungsform enthaltend probiotische Bakterien
US7662191B2 (en) * 2006-06-30 2010-02-16 össur hf Liner donning and doffing device
WO2008035332A1 (en) * 2006-09-19 2008-03-27 Technion Research And Development Foundation Ltd. Probiotic compositions and methods of making same
IL187645A (en) * 2007-11-26 2013-10-31 Degama Probiotics Ltd A process for making probiotic foods that can be baked
IL199781A0 (en) * 2009-07-09 2010-05-17 Yohai Zorea Heat resistant probiotic compositions and healthy food comprising them
MX2012001765A (es) * 2009-08-14 2012-03-07 Danisco Microorganismos deshidratados recubiertos con estabilidad y viabilidad mejoradas.

Also Published As

Publication number Publication date
NZ597968A (en) 2014-01-31
CN102482635B (zh) 2017-08-25
BR112012003239B1 (pt) 2021-03-16
BR112012003239A8 (pt) 2018-02-06
US8834853B2 (en) 2014-09-16
WO2011018509A1 (en) 2011-02-17
DK2464721T3 (en) 2016-01-25
CA2770168C (en) 2021-10-19
CN102482635A (zh) 2012-05-30
MX2012001765A (es) 2012-03-07
EP2464721A1 (en) 2012-06-20
EP2464721B1 (en) 2015-10-21
CN107087706A (zh) 2017-08-25
US20120142531A1 (en) 2012-06-07
CA2770168A1 (en) 2011-02-17
US9546352B2 (en) 2017-01-17
US20140349850A1 (en) 2014-11-27

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B25D Requested change of name of applicant approved

Owner name: DUPONT NUTRITION BIOSCIENCES APS (DK)

B15K Others concerning applications: alteration of classification

Ipc: C12N 1/04 (2006.01), A01N 63/00 (2006.01), A23K 10

B06T Formal requirements before examination [chapter 6.20 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06G Technical and formal requirements: other requirements [chapter 6.7 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 16/03/2021, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 14A ANUIDADE.