BR102015019623A2 - good enzymatic stability and high performance detergent composition for washing fabrics and surfaces - Google Patents

Abstract

1 / 1 Detergent composition with good enzymatic stability and high performance for washing fabrics and surfaces abstract the present invention is related to detergent compositions with good stability high performance enzyme for washing fabrics and surfaces comprising a combination of surfactants and at least one enzyme. for this, a composition is proposed which comprises a combination of surfactants comprising sodium lauryl ether sulfate (sles), amine oxide (ao) and at least one enzyme. in addition, the compounds cocamidopropyl betaane (capb) and sodium lauryl ether sulfosuccinate may be present in the combination of surfactants.

Description

"DETERGENT COMPOSITION OF GOOD ENZYMATIC STABILITY AND HIGH PERFORMANCE FOR WASHING FABRICS AND SURFACES" FIELD OF THE INVENTION

The present invention relates to good enzymatic stability and high performance detergent compositions for washing fabrics and surfaces comprising a combination of surfactants and at least one enzyme.

BACKGROUND OF THE INVENTION

[002] The surfactant compounds are amphiphilic substances, that is, they have in their molecular structure groups with antagonistic characteristics. In all surfactant molecules there is a polar group that has affinity for water (and other polar compounds), called a hydrophilic group. In the same molecule, there is also the so-called hydrophobic group, which by its very nature supports, has no affinity for water, but rather for oily substances (or, in general, supporting substances), often called lipophilic grouping.

In summary, surfactants are substances that modify surface and interfacial tensions, resulting in a number of related properties and applications.

Alkyl sulphates, together with alkyl ether sulphates are the most widely used products as foam surfactants in hair cosmetics, liquid soaps, oral hygiene products, among other applications. Among the alkyl sulfates, the most important are lauryl sulfates that have wide application in the cosmetic industry. The main characteristics of alkyl sulfates are their high foaming power, high viscosity reserve, good water solubility, pleasant odor and complete biodegradability.

Lauryl ether sulfates, because of their differentiated properties, are more hydrophilic than their non-ethoxylated counterparts, have low eye and skin irritability, low cloudiness, easy viscosity control from electrolyte addition, and increased resistance to water hardness.

Also, as anionic surfactants are alkyl sulfosuccinates, of excellent wetting power.

The prior art discloses some compositions used for cleaning produced from surfactant combinations.

[008] US 7,608,573 refers to the use of nonionic and anionic surfactants and others (solvent and short synthetic chain). The cleaning composition can be used for washing clothes and surfaces.

This reference claims a natural heavy-duty cleaning composition consisting of sodium lauryl sulfate, C12 oxide amine, C12-14 oxide amine at pH 7 to 13. In addition, the optional use of some materials as dyes, bleaches is mentioned. , thickeners and enzymes.

US2010 / 0184633 mentions the use of sulfoestolide surfactants as well as their derivatives and salts. The composition is useful for stain removal applications.

This patent claims the use of a sulfo-solid surfactant with at least one additional surfactant. It is mentioned that the secondary surfactant could be selected from a group of surfactants, including lauryl ether sulfonate. A group of surfactants to stabilize foams is further suggested, in which the use of lauryl cocoamidopropyl betaine and lauryl dimethyl amine oxide is mentioned.

[0012] US 2010/0234271 refers to the use of nonionic and anionic surfactants and others (solvent and short synthetic chain). The cleaning composition can be used for cleaning clothes, soft and hard surfaces and cleaners.

The composition consists of sodium lauryl sulfate and a hydrophobic synthetic surfactant selected from oxide amine. Optional ingredients such as enzymes, dyes, builders and fragrances are also described.

Further, it is mentioned that the composition does not contain some molecules, including alkyl ether sulfates, linear alkylbenzene sulfonate (LAS), linear alkylbenzene sulfonate acid (HLAS) or nonylphenol ethoxylate.

WO 2003/041667 describes a composition comprising a stabilized enzyme with at least 30% water. However, this reference aims to seek benefits in skin care (skin smoothness, skin softness, etc.). It is mentioned that the composition provides an enzyme-containing personal care product wherein the enzyme exhibits high stability and activity.

First, this foregoing reveals a stabilized enzyme composed of an osmoprotectant selected from the group comprising oxide amine. The use of protease as an enzyme and the use of enzyme inhibitor selected from the group of aryl boronic acid derivatives are described. It is described that the composition comprises a surfactant from the anionic, nonionic, zwitterionic amphoteric and cationic surfactant group. The types of surfactants are restricted to alkyl ether sulfates, alkyl amidopropyl betaines, alkyl betaines and the like, including mixtures thereof.

The document further discloses an enzyme-containing personal care product wherein said enzyme is stable in storage and active in the skin.

However, the art still needs the development of an efficient surfactant system for cleaning compositions, especially liquid detergent for washing fabrics and surfaces, which, in combination with enzymes, can provide superior performance and enzymatic and physical stability. usual detergent compositions found on the market and described in the prior art.

SUMMARY OF THE INVENTION

Broadly, the invention proposes a detergent composition comprising a combination of surfactants for use in washing fabrics and surfaces. The combination of surfactants comprises sodium lauryl ether sulfate (SLES), amine oxide (AO) and at least one enzyme. In addition, cocamidopropyl betaine (CAPB) and sodium lauryl ether sulfosuccinate compounds may also be present in the combination of surfactants. This combination ensures good enzymatic stability and better performance, allowing the use of a lower active content than commonly used on the market.

BRIEF DESCRIPTION OF THE FIGURES

The above and additional advantages will become clearer to those skilled in the art from the description below and the accompanying figures: Figure 1 shows a graph resulting from the enzyme stability test at 30 ° C for combinations of surfactants 1, 2 and 3 defined below.

Figure 2 shows a graph resulting from the enzymatic stability test at 37 ° C for surfactant combinations 1, 2 and 3.

Figure 3 presents a graph resulting from the oil and fat spot performance test of compositions containing surfactant combinations 1 and 2 prepared according to the present invention, and compositions 5 and 6 compatible with the state of the art.

Figure 4 shows a graph resulting from the protein performance test of surfactant combinations 1 and 2 prepared in accordance with the present invention, and prior art compositions 5 and 6.

Figure 5 is a graph resulting from the starch performance test of surfactant combinations 1 and 2 prepared according to the present invention and prior art compositions 5 and 6.

Figure 6 is a graph resulting from the complex spot performance test of surfactant combinations 1 and 2 prepared in accordance with the present invention, and prior art compositions 5 and 6.

Figure 7 shows a graph resulting from the particulate spot performance test of surfactant combinations 1 and 2 prepared in accordance with the present invention, and prior art compositions 5 and 6.

Figure 8 is a graph resulting from the pectin spot performance test of surfactant combinations 1 and 2 prepared according to the present invention and prior art compositions 5 and 6.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to a detergent composition comprising a combination of surfactants and at least one tissue and surface washing enzyme.

More specifically, the detergent composition of the present invention comprises a combination of surfactants comprising sodium lauryl ether sulfate (SLES) and amine oxide (AO), associated with at least one enzyme. Preferably, the at least one enzyme is selected from the group comprising protease, amylase, mannanase, lipase, pectin lyase and cellulase.

The sodium lauryl ether sulfate content ranges from 65 to 95% by weight, based on the total weight of the surfactant combination of the detergent composition of the present invention, while the amount of amine oxide ranges from 5 to 15% by weight. .

Optionally, the surfactant combination of the present invention further comprises sodium lauryl ether sulfosuccinate and / or cocamidopropyl betaine. When present, these additional surfactants are found in a range of 5 to 25% by weight.

Therefore, in surfactant combinations where the SLES content is less than 85% by weight, other surfactants such as sodium lauryl ether sulfosuccinate and cocamidopropyl betaine must be present in order to make up all the ingredients. 100% of surfactant combination.

The total content of the surfactant combination present in the detergent composition of the present invention ranges from 6% to 20% by weight, significantly lower than the contents of the commonly marketed compositions.

The detergent composition produced according to the present invention has a pH range ranging from 7 to 10.

To prove the inventiveness of the surfactant combination of the present invention, enzymatic stability and performance tests were performed, which are presented below.

ENZYMATIC STABILITY TEST

For the enzymatic stability test, three surfactant combinations were prepared with fixed mixing ratios: Combination 1: SLES / Oxide Amine (90/10);

Combination 2: SLES / Cocamidopropyl Betaine / Oxide Amine (80/10/10);

Combination 3: SLES / Sodium Lauryl Ether Sulfosuccinate / Oxide Amine (70/20/10).

These combinations were associated with the following enzymes: protease, amylase, mananase, lipase and pectin lyase, each tested individually.

For the enzyme stability test, all proposed runs (76 samples) were prepared under stability conditions as follows: - Temperature (° C): 30 and 37 - Time period (weeks): Start, 4 and 8 The pass criterion after 8 weeks was: - “Excellent” performance (green band) if results are above 70% to 100%; - “Satisfactory” performance (yellow band) if results are between 50% and 70%; - “Unacceptable” performance (red band) if results are below 50%.

The method for protease enzyme activity analysis was performed with the aid of Konelab 30 Analyzer at 0, 4 and 8 weeks and the results were provided in KNPU (S) SX.

First, the dimethyl casein compound present in the standard solution prepared for the test is hydrolyzed by the proteolytic enzyme to small peptides as shown below: The primary amino groups formed in this process react with trinitrobenzene sulfonic acid (TNBS) to form a complex. color as shown below: Complex color The development of this color is monitored by Konelab so that the change in absorption per unit of time can be calculated. This increase in absorption is proportional to the reaction rate and thus the activity of the enzyme.

For the test, the following reagents / substrates were prepared: - Dodecanol Polyoxyethylene Solution (Brij 35), 15% w / v - 2,4,6-Trinitrobenzene Sulfonic Acid Solution 0.2% w / v. - Sodium sulphite loading solution, NaS03 20% w / v (1.59 M NaS03). - 0.05 M borate buffer, pH 9.0. - N, N-Dimethyl casein 0.32%, pH 8.0 (DMC 0.32%). - 0.32% DMC with SO3, (0.064 M NaSO3), DMC-SX.

To prepare the standard solution, the following steps were taken: (a) Weigh an amount of standard enzyme corresponding to 2.40 units of KNUP (S) SX; b) Dissolve in 0.05 M borate buffer and pH 9.0 in a 500 mL volumetric flask; c) Shake on a magnetic stirrer for 30 minutes; d) The standard curve is a 7 point curve with a factor 4 between the lowest and highest standard points. The recommended total dilution volume is 1200 pL. Standard solutions are prepared by diluting the loading solution with 0.05 M borate buffer and pH 9.0 in a diluter directly into sample cups according to the table below: For sample preparation to be tested. , a known enzyme content was used and proceeded as described below: a) Weigh approximately 0.5 - 1.0 g of sample and dissolve in a volumetric flask with 0.5 M borate buffer and pH 9.0; b) Shake on a magnetic stirrer for 15 to 45 minutes; c) The samples are further diluted with 0.5 MepH 9.0 borate buffer to achieve an activity of approximately 0.30 mKNPU (S) SX / ml; d) Adding the surfactant combination according to the present invention.

After sample and reagent preparation, 0.32% DMC reagents with SO3 and 0.2% TNBS are placed in Konelab, along with the standard solution and sample.

The enzyme activity of the samples is determined with respect to a standard curve. Based on the abs / min results for standard enzymes, a standard with the activities of standard solutions in mKNPU (S) SX / mL is plotted on the x-axis of the graph, while the abs / min values of standard solutions are plotted on the y axis. To plot the graph, a logit / log4 algorithm is used.

The enzyme activity values of the diluted samples are taken from the standard curve. Sample activity is calculated using the following formula: Activity KNUP (S) SX n where S = reading the standard curve in mKNPU (S) SX / ml; V = Volume of the volumetric flask used in mL; F = Dilution factor for second dilution; W = sample weight in g; and 1000 = mKNPU (S) SX to KNPU (S) SX conversion factor.

[0053] The method for activity analysis of the enzymes amylase, mannanase, lipase and pectin lyase is also performed by the Konelab 30 Analyzer at 0, 4 and 8 weeks and results are provided in SNUX.

By this method, 1,4-alpha-D-glucanohydrolase in the samples and alpha-glucosidase enzyme in the reagents hydrolyze the substrate (4,6-ethylidene (G7) -p-nitrophenyl (Gi) -alpha-D -maltoeptaoside (ethylidene-G7PNP)) to glucose and yellowish-colored p-nitrophenol.

The rate of p-nitrophenol formation can be observed by Konelab 30. By expressing the reaction rate, it is possible to verify the enzymatic activity. etiHdeno-G ^ -p-oitrofeiiií-maitoeptaoside aifá-amiiase etüideno-Gn p-nitrophenyl alpha sicase i "glucose p-nitrophenol yellow, 405 min [0056] The following reagents / substrates were prepared for the test. : - Brij 35 solution, 15% w / v - 3M calcium chloride stabilizer with 0.25% Brij loading solution - 1% stabilizer (0.03M calcium chloride with 0.0025% Brij) ;

To prepare the standard solution, the following steps shall be taken: (a) Weighing a quantity of standard enzyme corresponding to 26,6 units of SNUX; (b) transfer the standard to a 250 ml volumetric flask containing approximately 200 ml of demineralized water; c) Add 2.5 ml of 3M calcium chloride stabilizer with 0.25% Brij loading solution and fill up to 250 ml with demineralized water (106.5 SNUX / ml); d) Shake on a magnetic stirrer for at least 5 minutes; e) From the loading solution the following standard solutions are prepared by dilution. The total volume is 1200 pL. The 1% stabilizer (0.03M calcium chloride with 0.0025% Brij) is used as the diluent for all subsequent dilutions. The standard curve is a 7 point curve with a factor 3 between the lowest and highest standard points.

The samples to be tested should be diluted at least 50 times and prepared as described below: a) Weigh approximately 1.0 g of sample and dissolve in a volumetric flask with demineralized water; (b) Add demineralized water equivalent to about three quarters of the volume of the volumetric flask; c) Add 3 M calcium chloride stabilizer with 0.25% Brij loading solution equivalent to 1% of the total volume of the flask and fill with demineralized water; d) Shake for approximately 15 minutes on a magnetic stirrer; e) The samples are further diluted with 1% stabilizer (3 M calcium chloride with 0.25% Brij loading solution) in the diluter. Dilution in the diluter is done directly in the sample vial; f) Adding the surfactant combination according to the present invention; g) Each weighing is analyzed once in the Konelab analyzer.

The analysis of the samples shall comprise the following steps: (a) pipetting 200 μΐ alpha-glucosidase into the cuvettes; (b) Pipette 16 μΐ of standard or sample solution into the cuvettes; (c) alpha-glucosidase and enzyme solution are incubated for 300 seconds; d) 20 μΐ of substrate is pipetted into the cuvettes. The glucosidase / amylase / substrate mixture is incubated for 180 seconds; e) Absorption is measured every 18 seconds. A total of seven absorption measurements are performed on each sample.

The activity of enzyme samples is determined with respect to a standard curve. A graph of the known concentration of the x-axis pattern versus the response of the Konelab 30 y-axis analyzer equipment is established by the Analytical Administration program using a logit / log4 algorithm.

Results are calculated automatically in the Analytical Administration program and the enzyme activity of the sample in SNUX / g is calculated using the following formula: S-V-F

[0062] SNUX activity / g = w. 1000, where S = reading the standard curve in mSNUX / ml; V = Volume of the volumetric flask in mL; F = Dilution factor for second dilution; W = sample weight in g; e 1000 = mSNUX to SNUX conversion factor.

Test results at 30 ° C are shown in the graph in Figure 1. It can be seen that surfactant combinations 1 and 2 show optimal results and surfactant combination 3 shows satisfactory results.

Figure 2 shows results for the enzymatic stability test at 37 ° C showing good stability of the surfactant combinations of the present invention even under severe temperature and time conditions.

PERFORMANCE TEST

For the performance test, two combinations of surfactants with fixed mixing ratios according to the present invention and two surfactants known from the state of the art were prepared.

The surfactant combinations prepared according to the present invention are presented below: 1) SLES / Oxide Amine (90/10); 2) SLES / Cocamidopropyl Betaine / Oxide Amine (80/10/10).

The surfactants known in the art and used in the test for comparative purposes are: 5) Linear Sodium Alkylbenzene Sulfonate / Sodium Alkyl Ether Sulfate / Alkylpolyoxy-6) Linear Sodium Alkylbenzene Sulfonate / Alkyl Ether Sodium Sulfate / Non-surfactant Polyalkylethoxylate [0068] The test was performed to compare the performance of surfactants with respect to cleaning stains that are most common to consumers. Stains were grouped to balance the stain load, avoiding excessive concentration of dirt in the same group. The list of spots used in the test is presented below: It is important to note that the same enzymes were employed in the test in order to make the comparison possible.

Figure 3 shows the performance result in the oil and fat group through the bloxplot graph. It is noted that the performance of surfactant combinations 1 and 2 is clearly superior to surfactants 5 and 6 known in the art.

The same conclusion can be seen in the graphs of Figures 4 to 8.

From the results of the demonstrated tests, it can be concluded that the surfactant combinations prepared according to the present invention far outperform the surfactants known in the art with respect to various types of stains and dirt present on the fabric and surface.

Claims (10)

1. A detergent composition of good enzymatic stability and high performance for surface and fabric washing, characterized in that it comprises a combination of surfactant comprising sodium lauryl ether sulfate (SLES) and amine oxide (AO), and at least one enzyme. Composition according to Claim 1, characterized in that it further includes sodium lauryl ether sulfosuccinate. Composition according to Claim 1 or 2, characterized in that it further includes cocamidopropyl betaine (CAPB). Composition according to Claim 1, characterized in that the content of SLES in the surfactant combination ranges from 65 to 95% by weight. Composition according to Claim 1 or 2, characterized in that the AO content in the surfactant combination ranges from 5 to 15% by weight. Composition according to Claim 2 or 3, characterized in that the content of the additional surfactants sodium lauryl ether sulfosuccinate and / or CAPB in the surfactant combination ranges from 5 to 25% by weight. Composition according to any one of the preceding claims, characterized in that the enzyme is selected from the group consisting of: protease, amylase, mannanase, lipase, pectin lyase and cellulase. Composition according to any one of the preceding claims, characterized in that the content of the surfactant combination of the detergent composition is from 6% to 20% by weight. 9 Composition according to any one of the preceding claims, characterized in that the pH range ranges from 7 to 10.