CN108251402A - The foam separation method of lipase in a kind of harvesting aqueous solution - Google Patents
The foam separation method of lipase in a kind of harvesting aqueous solution Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
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Abstract
The foam separation method of lipase, is related to foam fraction factor in a kind of harvesting aqueous solution.It checks foam separator, determines without leak, without gas leak phenomenon；Test the performance of different surfaces activating agent；Prepare lipase solution to be separated；Fatty enzyme aqueous solution is added in the foam generator tube of device, open valve, pressure reducing valve, regulating valve and the shut-off valve of Air compressing bottle, air is exported by compression bottle, pass through the pressure gauge and spinner flowmeter on compression bottle, the gas flow rate of branch needed for adjusting, gas arrives at the bottom of foam generator tube, it is uniformly distributed and is delivered up on air sparger, so as to drive, detach the solution's solute in foam generator tube, bubble is discharged by foam generator tube top, and be collected into receiving flask, raffinate is then discharged by raffinate dump valve；Sample sealing after collection, stands defoaming, measures foam solution volume and the concentration of lipase；Value and the mapping of rate of recovery R and concentration ratio E is calculated according to the data obtained；Clean foam generator tube.
Description
Technical field
The present invention relates to foam fraction factor, more particularly, to using natural surfactant coconut oil saponin(e (CAPB) as blistering
The foam separation method of lipase in a kind of harvesting aqueous solution of agent.
Background technology
Lipase (Lipase) is a kind of irreversible enzyme, i.e., in addition to the reaction that can be catalyzed grease decomposition, moreover it is possible to be catalyzed the conjunction of ester
Into.In addition, lipase-catalyzed chemical reaction also has, specificity is good, catalytic efficiency is strong, reaction condition does not stimulate, stereoselectivity
Well, by-product is few and to the features such as instrument and equipment requirement is not very high.Therefore, in numerous industrial circles, such as food, skin
Leather, cosmetics, detergent, biomedicine, biomaterial, biosensor etc. have larger development[1-6].Lipase is in industry
Upper another big application is production biodiesel[7].The conventional acquisition method of lipase mainly has membrane filtration and liquid-liquid extraction, but film
Easily contaminated, the replacement of film is more frequent, and the manufacture cost of film in itself is also very high；And the method extraction protein extracted is past
Toward the phenomenon that will appear emulsification, it is deteriorated so as to cause separating effect.Cost to be isolated and purified in lipase production process too high at present
It restricts its application, how to reduce its production cost becomes the emphasis of lipase industrial research[8-10]。
Foam separating technology is according to adsorption principle, when foam is from the generation of separation column bottom, the strong object of surface-active
Matter can be adsorbed on foam, and and then foam rises together, and the weaker substance of surface-active can then stay in a liquid, and foam is final
Froth bed is formed above liquid, finally by collection froth bed, it will be able to be enriched to the strong substance of surface-active, reach selection
Effect[11-15].Foam separating technology is because it has low cost, high efficiency and environment amenable advantage, in wastewater treatment, food
The fields such as product, biology, medicine, chemical industry receive very big concern[16-18].In consideration of it, investigating foamet herein collects fat
The technique of enzyme.
Bibliography：
[1] research of Liu Honglei, Miao Ming, Jiang Bo, et al. microbial lipases and application [J] food industry science and technology,
2012,12):376-381.
[2] Liu Ying, the safe China of wood, Sun Hongnan, application present situation of the et al. foam separating technologies in food and chemical industry
[J] food industry science and technology, 2013,13):354-358.
[3] progress of Cao Qian, Feng Feng qin microbial lipases and its application [J] Chinese foods in food
Report, 2013,10):136-143.
[4] research [D] of Xue Yong microbial lipases and its catalytically synthesizing biological diesel oil:The Central China University of Science and Technology, 2004:
Pages.
[5] Yang Jing fat enzyme immobilizatio and its application [D] in butyl oleate synthesis:Beijing University of Chemical Technology,
2008:Pages.
[6] Yu Ying lipase immobilizations and its application study [D]:Southern Yangtze University, 2007:Pages.
[7] Zhu Junren, Zheng Xuxu, Li Qiang, the lipase-catalyzed progress for preparing biodiesel of et al. separate sources
[J] China agronomy notification, 2010,04):318-322.
[8]FUKUDA H,KONDO A,NODA H.Biodiesel fuel production by
transesterification of oils[J].Journal of Bioscience and Bioengineering,2001,
92(5):405-416.
[9]VAISALI C,BELUR P D,REGUPATHI I.Lipase mediated synthesis of rutin
fatty ester:Study of its process parameters and solvent polarity[J].Food
chemistry,2017,232(278-285.
[10]ZIMMERMANN R,STRAUSS J G,HAEMMERLE G,et al.Fat mobilization in
adipose tissue is promoted by adipose triglyceride lipase[J].Science,2004,306
(5700):1383-1386.
[11] Ma Jiahua, Tan Chengjia, Han Li, et al. foamets are in research [J] of dung beetle Separation of Proteins
Herbal medicine, 2009,08):1223-1225.
[12] mechanism and application study [D] of the Zhang Dongmei based on foamet processing heavy-metal stain compound wastewater:Lake
Southern university, 2012:Pages.
[13] Liu Zhihong, Liu Zheng, Ding Fuxin, et al. are concentrated with foamet and protein isolate matter (I) --- equipment
And research [J] Tsinghua University's journals (natural science edition) of separation process, 1997,12):52-56.
[14] Wei Fengyu, Zhang Jing, Xie Hui foamets purifying sapindoside [J] Chinese patent drugs, 2009,07):
1021-1024.
[15] king's foundation foamet protein isolates Quality Research [D]:University Of Tianjin, 2004:Pages.
[16]Ahmad S I.Laws of foam formation and foam fraction 1.The effect
of different operationg parameters on the foam fractionation of albumin from
a solution containing organic and inorganic materials[J].Sep Sci,1975,10:673-
688.
[17]Christopher E L,Paul M B,Michael J.Purification of proteins using
foam fractionation[J].Pharmaceutical Research,1997,14(11):1511-1515.
[18]Stevenson P.Hydrodynamic theory of rising foam[J].Minerals
Engineering,2007,20:282-289.
[19] Su Yan peaches foamets concentrate, detach the research [D] of saponin component in licorice water extract:In Chengdu
Medical pharmaceutical university, 2006:Pages.
[20] surfactant and metal ion [D] in Xu Yun foamets removal waste water:University Of Tianjin, 2012:
Pages.
Invention content
The purpose of the present invention is to provide one kind using natural surfactant coconut oil saponin(e (CAPB) as foaming agent
Harvest the foam separation method of lipase in aqueous solution.
The present invention includes the following steps：
1) it checks foam separator, determines without leak, without gas leak phenomenon；
In step 1), the foam separator is equipped with Air compressing bottle, pressure reducing valve, pressure gauge, regulating valve, cut-off
Valve, spinner flowmeter, air sparger, foam generator tube, receiving flask and raffinate dump valve；The outlet warp of the Air compressing bottle
Pressure reducing valve and pressure gauge connect the input terminal of regulating valve, the input terminal of the output termination shut-off valve of regulating valve, the output terminal of shut-off valve
Connect air sparger and raffinate dump valve, the input of the output termination foam generator tube of air sparger respectively through spinner flowmeter
End, the output terminal of foam generator tube connect receiving flask, the outlet discharge raffinate of raffinate dump valve.
2) performance of different surfaces activating agent is tested；
In step 2), the specific method of the performance of the test different surfaces activating agent can be：Different surfaces are prepared to live
Property agent solution, test different surfaces activating agent performance；The different surfaces activator solution may include natural non-ionic table
Face activating agent coconut oil foaming agent (CAPB), cationic surface active agent cetyl trimethylammonium bromide (CTAB), it is cloudy from
Subtype surfactant sodium dodecyl base sodium sulfonate (DBS) and neopelex (SDBS), nonionic surface active agent
Positive certain herbaceous plants with big flowers base glucoside (C10APG), the molar concentration of the different surfaces activator solution is 0.001mol/L.
3) lipase solution to be separated is prepared；
In step 3), the specific method of preparation lipase solution to be separated can be：Prepare the fat of various concentration
Fat enzyme aqueous solution adds in surfactant, with 6mol/L and 1mol/L NaOH solutions, 6mol/L and 1mol/L HCl solution tune
Save solution ph.
4) fatty enzyme aqueous solution is added in the foam generator tube of foam separator, open Air compressing bottle valve,
Pressure reducing valve, regulating valve and shut-off valve, air are exported by compression bottle, and are divided into two-way, pass through the pressure gauge and rotor on compression bottle
Flowmeter, the gas flow rate of branch needed for adjusting, gas arrive at the bottom of foam generator tube, are uniformly distributed on air sparger
And be delivered up, so as to drive, detach the solution's solute in foam generator tube, bubble is finally discharged by foam generator tube top,
And be collected into receiving flask, raffinate is then discharged by raffinate dump valve；
5) the sample sealing after collecting, stands defoaming, measures foam solution volume and the concentration of lipase；
In step 5), preservative film sealing can be used in the sealing.
6) value and the mapping of rate of recovery R and concentration ratio E is calculated according to the data obtained；
7) foam generator tube is cleaned.
In step 7), the cleaning foam generator tube can be cleaned first with tap water, then with distilled water flushing at least 1 time,
Finally dry.
The present invention determines foam fraction factor using natural surfactant coconut oil saponin(e (CAPB) as foaming agent and trapping agent
The optimum process condition of lipase is harvested, using natural surfactant coconut oil saponin(e (CAPB) as foaming agent and trapping agent,
By repeating to test, foundation is used as using the lipase rate of recovery and concentration ratio, it is determined that the best work of foam fraction factor harvesting lipase
Skill condition：Under room temperature, fatty enzyme aqueous solution pH is 9, separation gas velocity is 600mL/min, the blistering of liquid amount 300mL, coconut oil
Agent concentration is 0.035g/L, initial enzyme concentration is 5g/L.At this point, lipase concentration ratio E=5.83, rate of recovery R=98.18%.
Description of the drawings
Fig. 1 is the structure composition schematic diagram of foam separator described in the embodiment of the present invention.
Fig. 2 is 5 kinds of surfactant various concentrations to interfacial tension.
Fig. 3 is influence of the initial enzyme concentration to concentration ratio and the rate of recovery.
Fig. 4 is influence of the air velocity to concentration ratio and the rate of recovery.
Fig. 5 is influence of the surfactant concentration to concentration ratio and the rate of recovery.
Fig. 6 is influence of the liquid amount to concentration ratio and the rate of recovery.
Fig. 7 is influence of the pH value to concentration ratio and the rate of recovery.
Specific embodiment
Following embodiment will the present invention is further illustrated with reference to attached drawing.
Referring to Fig. 1, the foam separator embodiment is equipped with Air compressing bottle 1, pressure reducing valve 2, pressure gauge 3, regulating valve
4th, shut-off valve 5, spinner flowmeter 6, air sparger 7, foam generator tube 8, receiving flask 9 and raffinate dump valve 10；The air
The outlet of compression bottle 1 connects the input terminal of regulating valve 4 through pressure reducing valve 2 and pressure gauge 3, and the output of regulating valve 4 terminates the defeated of shut-off valve 5
Enter end, the output terminal of shut-off valve 5 connects air sparger 7 and raffinate dump valve 10, air sparger 7 respectively through spinner flowmeter 6
Output termination foam generator tube 8 input terminal, the output terminal of foam generator tube 8 connects receiving flask 9, the outlet of raffinate dump valve 10
Discharge raffinate.
Specific embodiment is given below.
Embodiment 1
The present embodiment is the type for changing surfactant, and fixed other experimental factors compare different surfaces activating agent
The stability of foamability and foam, determines optimum surfactant.
Step：
Prepare 5 kinds of solution：Surfactant CAPB a concentration of 0.001g/L, C10APG a concentration of 0.001g/L, SDBS is dense
It spends for a concentration of 0.001g/L of 0.001g/L, DBS a concentration of 0.001g/L, CTAB.Using the foam separator of Fig. 1, to bubble
It is 200mL that amount of liquid is added in foam generator tube, and adjusting air velocity is 100mL/min, experimental period 1min, observes and measures gas
Lifting height is steeped, this is highly denoted as to blistering elemental height, to represent surfactant foamability；Then respectively 0min,
0.5min, 1min, 1.5min, 2min, 3min, 3.5min record foam height, stablize with the ratio between foam elemental height for blistering
The measurement of property.Experiment is carried out and is repeated 3 times at the same temperature, by results are averaged.The surface of 5 kinds of surfactants
Power-concentration map is as shown in Fig. 2, 5 kinds of surfactant foamabilities and foaming stability measurement result are as shown in table 1,5 kinds of surfaces
Activating agent foam height and its time used, the results are shown in Table 2.
Table 1
Table 2
It is by can be seen that 5 kinds of surfactants reduce surface tension capacity of water in table 1：C10APG>CAPB>SDBS>
CTAB>DBS, CAPB reduce the nonionic surfactant C10APG that interfacial tension ability is weaker than same type, are better than other chemistry
Ionic surfactant, and concentration is minimum.This shows that CAPB has great advantage in foaming characteristic and dosage.It, can by table 2
The foamability size for finding out 5 kinds of surfactants is：C10APG>CAPB>SDBS>CTAB>DBS, with reducing surface above
Power ability is consistent.Foaming stability difference causes final foam height different.In the range of experiment pipe range, C10APG, CAPB,
CTAB foamabilities and stability are all fine.The foamability of SDBS compares that former three is weaker, and in the 1min finally measured,
Its rate of climb starts the speed that slightly below front rises.And DBS has self-defoaming phenomenon in measurement process, can be seen by data
Go out, foamability and stability are also worst.
To sum up, the foamability of CAPB and the stability of foam are very good, and CAPB is accorded with as a kind of natural extract
The theory of the present invention is closed, therefore selects CAPB as optimum surfactant.
Embodiment 2
The present embodiment is to change initial lipase concentration in foam fraction factor system, and fixed other empirical factors are more different
The rate of recovery and concentration ratio of lipase under initial enzyme concentration determine the initial enzyme concentration of lipase in separation harvesting aqueous solution.
Step：
Prepare lipase solution concentration be respectively 5,10,15,20g/L.Using Fig. 1 devices, added in into foam generator tube
Amount of liquid is 200mL, adjusting gas velocity is 600mL/min, carries out foam fraction factor harvesting aqueous solution lipase experiment, and acquisition time is
15min, experimental result are as shown in Figure 3.
By Fig. 3 data analyses, as initial enzyme concentration concentration increases, the rate of recovery increases and concentration ratio reduces, in initial enzyme
Under the conditions of a concentration of 5g/L, concentrate output is had no.When enzyme solutions concentration is low, the effect of foam fraction factor is more preferable, but lipase drops
Low surface tension ability is limited, therefore foam is less and stability is poor, causes the rate of recovery low.Therefore, initial enzyme concentration is chosen
Under the conditions of 5g/L, after adding in surfactant, foam fraction factor harvesting lipase process conditions are investigated.
Embodiment 3
The present embodiment is the separation gas velocity changed in foam fraction factor system, fixed other empirical factors, more different separation
The rate of recovery and concentration ratio of lipase under gas velocity determine the separation gas velocity of optimal separation harvesting aqueous solution lipase.
Step：
It is a concentration of 0.040g/L of 0.5g/L, CAPB to prepare the initial lipase concentration of solution, using Fig. 1 devices, to foam
It is 200mL that amount of liquid is added in generator tube, adjust air gas velocity be followed successively by 200,400,600,800,1000mL/min, steeped
The experiment of foam separation harvesting aqueous solution lipase, acquisition time 15min, experimental result are as shown in Figure 4.
It is analyzed by Fig. 4 it is found that concentration ratio increases with the increase of air velocity, and the rate of recovery is with the increasing of air velocity
It reduces greatly.Air velocity is low, and the foamability of solution is poor, and concentration ratio is low, although and the bubble that is generated under upper air current speed increases
It ruptures mostly but quickly, makes the concentration liquid measure finally collected very little, cause the rate of recovery relatively low.Consider, optimum air flow velocity is determined
For 600mL/min.
Embodiment 4
The present embodiment is the concentration for changing surfactant in foam fraction factor system, fixed other empirical factors, than less
With the rate of recovery and concentration ratio of lipase under surfactant concentration, determine that the surface of optimal separation harvesting aqueous solution lipase is lived
Property agent concentration.
Step：
Prepare solution initial lipase concentration for 5g/L, CAPB concentration be respectively 0.005,0.010,0.0175,0.035g/
L, using Fig. 1 devices, it is 200mL that amount of liquid is added in into foam generator tube, and it is 600mL/min to adjust air gas velocity, is steeped
The experiment of foam separation harvesting aqueous solution lipase, acquisition time 15min.Experimental result is as shown in Figure 5.
The experimental results showed that：Lipase concentration ratio is reduced as surfactant concentration increases, and the rate of recovery is with surface
Surfactant concentration increases and increases.Surfactant concentration is too low, and the foam of generation is unstable, and enough foams to be generated arrive
During tower top, the moisture in foam has been lost in mostly, thus be enriched with it is higher, but due to foam volume is few and the rate of recovery is relatively low.This
Invention chooses 0.035g/L as optimum surfactant concentration, the rate of recovery 39.2%.
Embodiment 5
The present embodiment is the liquid amount changed in foam fraction factor system, fixed other empirical factors, more different liquid amounts
The rate of recovery and concentration ratio of lower lipase determine the liquid amount of optimal separation harvesting aqueous solution lipase.
Step：
It is a concentration of 0.035g/L of 5g/L, CAPB to prepare solution initial embodiment enzyme concentration, using Fig. 1 devices, to foam
Added in generator tube amount of liquid for be respectively 50,100,200,300,400mL, adjust air gas velocity and steeped for 600mL/min
The experiment of foam separation harvesting aqueous solution lipase, acquisition time 15min, experimental result are as shown in Figure 6.
The experimental results showed that：If the height of foam separating tower is fixed, different separation stoste volumes can influence foam
The ratio of layer height and liquid layer height, and then influence to be adsorbed onto surface active molecules, foaming water discharge and the foam of gas-liquid interface
Separation.When liquid amount is smaller, foam is longer the time required to rising so that foam being capable of more abundant draining；And liquid amount increases, bubble
Residence time increases foam in the liquid phase so that lipase is more adsorbed on boundary.Fig. 6 shows to increase with liquid amount
Add, lipase concentration ratio and recycling take the lead in increasing and then reduce.When liquid amount is 300mL, concentration ratio and the rate of recovery reach maximum
Value, respectively 4.39 and 66.39%, therefore it is optimum condition that selection liquid amount, which is 300mL,.
Embodiment 6
The present embodiment is the pH value for changing the initial soln in foam fraction factor system, fixed other empirical factors, than less
With the rate of recovery and concentration ratio of lipase under pH, the pH value of optimal separation harvesting aqueous solution lipase is determined.
Step：
It is a concentration of 0.035g/L of 5g/L, CAPB to prepare the initial lipase concentration of solution, using Fig. 1 devices, is sent out to foam
It is 300mL that amount of liquid is added in raw pipe, pH value of solution is respectively 3,5,7,9,11, adjust air gas velocity for 600mL/min, steeped
Foam separation harvesting aqueous solution lipase experiment, experimental result are as shown in Figure 7.
It can be obtained by the data analysis of Fig. 7, when pH value is less than 9, the concentration ratio and the rate of recovery of lipase are with the increasing of pH value
Add and increase；When pH value is higher than 9, the concentration ratio and the rate of recovery of lipase reduce instead with the increase of pH value.When pH value is 9
When, concentration ratio and the rate of recovery respectively reach highest, respectively reach 5.83 and 98.18%.At isoelectric point, protein molecule institute band
Electrostatic charge be zero, intermolecular opposing force is minimum, and the surface tension of liquid is minimum, and hydrophobicity is most strong, it is easier to adsorb
Gas-liquid interface simultaneously forms stable foam, and so as to improve accumulation rate and the rate of recovery, separating effect tends to be optimal.When pH value is 9,
Bubble stability is best.PH value differs bigger with 9, and the stability of bubble is poorer；When pH value is 3, foam defoams rapidly after generating.
Therefore pH value for 9 be lipase foam fraction factor optimum condition.
Analysis method is given below：
1st, solution lipase separating effect evaluation index
The extraction effect of lipase uses concentration ratio, zymoprotein collection rate as evaluation index, as follows.
Concentration ratio：
The zymoprotein rate of recovery：
C in formula1For the foam solution protein concentration of collection, g/L；C0For the Lipase protein concentration in initial liquid, g/L；V1For
Foam solution volume, L；V2It is accumulated for original liquid[19]。
2nd, in solution the concentration of lipase measure
Protein determination uses Coomassie brilliant blue spectrophotometric Colo.
Method is consistent with the method for surveying standard items, is filled after adding in 20 μ L unknown samples in 200 μ L Coomassie brilliant blue reagents
Divide mixing, and probably to know the concentration of unknown sample, it is impossible to beyond the linear extent of standard curve.Further according to what is measured
OD595nm values, combined standard curve calculate the amount of standard protein, so as to calculate the protein concentration of sample (mg/mL).
Claims (7)
1. a kind of foam separation method for harvesting lipase in aqueous solution, it is characterised in that include the following steps：
1) it checks foam separator, determines without leak, without gas leak phenomenon；
2) performance of different surfaces activating agent is tested；
3) lipase solution to be separated is prepared；
4) fatty enzyme aqueous solution is added in the foam generator tube of foam separator, opens valve, the decompression of Air compressing bottle
Valve, regulating valve and shut-off valve, air are exported by compression bottle, and are divided into two-way, pass through the pressure gauge and spinner on compression bottle
Meter, the gas flow rate of branch needed for adjusting, gas arrive at foam generator tube bottom, be uniformly distributed on air sparger and to
Upper conveying, so as to drive, detach the solution's solute in foam generator tube, bubble is finally discharged by foam generator tube top, and is received
Collect in receiving flask, raffinate is then discharged by raffinate dump valve；
5) the sample sealing after collecting, stands defoaming, measures foam solution volume and the concentration of lipase；
6) value and the mapping of rate of recovery R and concentration ratio E is calculated according to the data obtained；
7) foam generator tube is cleaned.
2. a kind of foam separation method for harvesting lipase in aqueous solution as described in claim 1, it is characterised in that in step 1)
In, the foam separator is equipped with Air compressing bottle, pressure reducing valve, pressure gauge, regulating valve, shut-off valve, spinner flowmeter, air
Distributor, foam generator tube, receiving flask and raffinate dump valve；The outlet of the Air compressing bottle connects tune through pressure reducing valve and pressure gauge
The input terminal of valve, the input terminal of the output termination shut-off valve of regulating valve are saved, the output terminal of shut-off valve connects respectively through spinner flowmeter
Air sparger and raffinate dump valve, air sparger output termination foam generator tube input terminal, foam generator tube it is defeated
Outlet connects receiving flask, the outlet discharge raffinate of raffinate dump valve.
3. a kind of foam separation method for harvesting lipase in aqueous solution as described in claim 1, it is characterised in that in step 2)
In, the specific method of the performance of the test different surfaces activating agent is：Different surfaces activator solution is prepared, tests different tables
The performance of face activating agent；The different surfaces activator solution may include natural nonionic surface active agent coconut oil blistering
Agent, cationic surface active agent cetyl trimethylammonium bromide, anionic surfactant dodecyl sodium sulfate and
The positive certain herbaceous plants with big flowers base glucoside of neopelex, nonionic surface active agent.
A kind of 4. foam separation method for harvesting lipase in aqueous solution as claimed in claim 3, it is characterised in that the difference
The molar concentration of surfactant solution is 0.001mol/L.
5. a kind of foam separation method for harvesting lipase in aqueous solution as described in claim 1, it is characterised in that in step 3)
In, the specific method of preparation lipase solution to be separated is：The fatty enzyme aqueous solution of various concentration is prepared, adds in surface
Activating agent adjusts solution ph with 6mol/L and 1mol/L NaOH solutions, 6mol/L and 1mol/L HCl solutions.
6. a kind of foam separation method for harvesting lipase in aqueous solution as described in claim 1, it is characterised in that in step 5)
In, the sealing is sealed using preservative film.
7. a kind of foam separation method for harvesting lipase in aqueous solution as described in claim 1, it is characterised in that in step 7)
In, the cleaning foam generator tube is first cleaned with tap water, then with distilled water flushing at least 1 time, is finally dried.
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| CN111593033A (en) * | 2020-04-24 | 2020-08-28 | 厦门大学 | Foam separation method of lipase in fermentation liquor |
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