BR0318667A - promotor bidirecional, plantas transgênicas e vetor de transformação de planta - Google Patents
promotor bidirecional, plantas transgênicas e vetor de transformação de plantaInfo
- Publication number
- BR0318667A BR0318667A BRPI0318667-9A BR0318667A BR0318667A BR 0318667 A BR0318667 A BR 0318667A BR 0318667 A BR0318667 A BR 0318667A BR 0318667 A BR0318667 A BR 0318667A
- Authority
- BR
- Brazil
- Prior art keywords
- transcription
- module
- bidirectional
- directions
- dna sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
"PROMOTOR BIDIRECIONAL, PLANTAS TRANSGêNICAS E VETOR DE TRANSFORMAçãO DE PLANTA". A presente invenção descreve um módulo bidirecional para a ativação de expressão de gene e a regulação da transcrição em ambas as direções. O módulo bidirecional compreende múltiplos elementos de seqüência de DNA cis regulatórios, estrategicamente dispostos para proporcionar um 'Módulo de Ativação de Transcrição' que alcança um elevado nível de expressão a partir do 'Módulo de Iniciação de Transcrição'. O último funciona como um promotor mínimo. O primeiro ativa a transcrição simultaneamente em ambas as direções a partir da última e também responde a diversas transcrições, induzindo o estímulo externo em ambas as direções. Uma vez que o mesmo é um módulo de transcrição bidirecional artificialmente projetado, o mesmo não apresenta uma seqüência de DNA equivalente no genoma vegetal. Isto reduz as chances dos genes serem silenciados por mecanismos com base em homologia. Um módulo promotor bidirecional como tal, pode portanto ser usado para desenvolver vetores eficientes para engenharia genética em plantas.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IN2003/000442 WO2005063993A1 (en) | 2003-12-31 | 2003-12-31 | Artificial bidirectional promoter for activation of gene expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0318667A true BR0318667A (pt) | 2006-11-28 |
Family
ID=34717559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0318667-9A BR0318667A (pt) | 2003-12-31 | 2003-12-31 | promotor bidirecional, plantas transgênicas e vetor de transformação de planta |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1702070B1 (pt) |
| JP (1) | JP4456076B2 (pt) |
| CN (1) | CN1886510B (pt) |
| AT (1) | ATE482277T1 (pt) |
| AU (1) | AU2003304662B2 (pt) |
| BR (1) | BR0318667A (pt) |
| DE (1) | DE60334329D1 (pt) |
| WO (1) | WO2005063993A1 (pt) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140107334A (ko) * | 2011-12-30 | 2014-09-04 | 다우 아그로사이언시즈 엘엘씨 | 합성 양방향성 식물 프로모터 ｕｂｉ１을 위한 구축물 및 방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN192829B (pt) * | 1998-11-09 | 2004-05-22 | Council Scient Ind Res | |
| CN1230592A (zh) * | 1999-03-22 | 1999-10-06 | 中国科学院遗传研究所 | 棉花曲叶病毒启动子及其应用 |
| US6388170B1 (en) * | 2000-04-07 | 2002-05-14 | University Of Kentucky Research Foundation | Bidirectional promoters and methods related thereto |
-
2003
- 2003-12-31 CN CN2003801109425A patent/CN1886510B/zh not_active Expired - Fee Related
- 2003-12-31 JP JP2005512741A patent/JP4456076B2/ja not_active Expired - Fee Related
- 2003-12-31 AT AT03819194T patent/ATE482277T1/de not_active IP Right Cessation
- 2003-12-31 WO PCT/IN2003/000442 patent/WO2005063993A1/en active IP Right Grant
- 2003-12-31 EP EP03819194A patent/EP1702070B1/en not_active Expired - Lifetime
- 2003-12-31 AU AU2003304662A patent/AU2003304662B2/en not_active Ceased
- 2003-12-31 DE DE60334329T patent/DE60334329D1/de not_active Expired - Lifetime
- 2003-12-31 BR BRPI0318667-9A patent/BR0318667A/pt not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1702070B1 (en) | 2010-09-22 |
| CN1886510B (zh) | 2011-04-06 |
| ATE482277T1 (de) | 2010-10-15 |
| AU2003304662B2 (en) | 2007-08-02 |
| AU2003304662A1 (en) | 2005-07-21 |
| JP2007518392A (ja) | 2007-07-12 |
| EP1702070A1 (en) | 2006-09-20 |
| WO2005063993A1 (en) | 2005-07-14 |
| CN1886510A (zh) | 2006-12-27 |
| JP4456076B2 (ja) | 2010-04-28 |
| DE60334329D1 (de) | 2010-11-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: INDEFIRO O PEDIDO DE ACORDO COM O ART. 8O COMBINADO COM ART. 11, ART. 18 E ART. 25 DA LPI. |
|
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL. |