BR0209095A - Monitoração baseada em pcr de sistemas de biotratamento de água residual - Google Patents

Monitoração baseada em pcr de sistemas de biotratamento de água residual

Info

Publication number
BR0209095A
BR0209095A BR0209095-3A BR0209095A BR0209095A BR 0209095 A BR0209095 A BR 0209095A BR 0209095 A BR0209095 A BR 0209095A BR 0209095 A BR0209095 A BR 0209095A
Authority
BR
Brazil
Prior art keywords
pcr
gene
indicator
effector
abundance
Prior art date
Application number
BR0209095-3A
Other languages
English (en)
Inventor
David B Carson
James F Rice
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of BR0209095A publication Critical patent/BR0209095A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/006Regulation methods for biological treatment
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"MONITORAçãO BASEADA EM PCR DE SISTEMAS DE BIOTRATAMENTO DE áGUA RESIDUAL". A presente invenção refere-se à monitoração baseada em PCR de microorganismos em sistemas para o tratamento biológico de água residual. Em particular, medição de ambas, a abundância e expressão de genes indicadores/efetores ou combinações de genes, onde expressão de um gene "efetor" correlaciona-se com a atividade degradante de uma particular amostra microbial,e onde a abundância de um gene "indicador" correlaciona-se com a abundância de micróbios. Expressão de gene efetor é medida por RT-PCR quantitativa competitiva, e abundância de gene indicador é medida por PCR quantitativa competitiva. Os genes indicadores e efetores podem ser genes idênticos ou diferentes. Em uma realização, o gene indicador e efetor são seq³ências do gene glifosato oxidorredutase (gox) e as técnicas quantitativas empregadas são PCR quantitativa competitiva e RT-PCR quantitativa competitiva.
BR0209095-3A 2001-04-23 2002-04-23 Monitoração baseada em pcr de sistemas de biotratamento de água residual BR0209095A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28584601P 2001-04-23 2001-04-23
PCT/US2002/012923 WO2002085791A2 (en) 2001-04-23 2002-04-23 Pcr-based monitoring in wastewater biotreatment systems

Publications (1)

Publication Number Publication Date
BR0209095A true BR0209095A (pt) 2004-07-13

Family

ID=23095951

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0209095-3A BR0209095A (pt) 2001-04-23 2002-04-23 Monitoração baseada em pcr de sistemas de biotratamento de água residual

Country Status (9)

Country Link
US (1) US6849430B2 (pt)
EP (1) EP1414750A4 (pt)
CN (1) CN1268767C (pt)
AR (1) AR035832A1 (pt)
AU (1) AU2002257205B2 (pt)
BR (1) BR0209095A (pt)
CA (1) CA2444316A1 (pt)
MX (1) MXPA03009732A (pt)
WO (1) WO2002085791A2 (pt)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040161767A1 (en) * 2002-06-28 2004-08-19 Baldwin Brett R. Detection and quantification of aromatic oxygenase genes by real-time PCR
US7303676B2 (en) * 2003-02-13 2007-12-04 Zenon Technology Partnership Supported biofilm apparatus and process
US7175763B2 (en) * 2003-02-13 2007-02-13 Zenon Technology Partnership Membrane supported biofilm process for autotrophic reduction
US7300571B2 (en) * 2003-02-13 2007-11-27 Zenon Technology Partnership Supported biofilm apparatus
JP2006518661A (ja) * 2003-02-13 2006-08-17 ゼノン、エンバイロンメンタル、インコーポレーテッド 支持されたバイオフィルム装置と方法
BRPI0709282B8 (pt) 2006-03-29 2021-05-25 Merial Ltd vacina contra estreptococos
GB2438216B (en) * 2006-05-17 2008-11-19 Schlumberger Holdings Methods and systems for evaluation of hydrocarbon reservoirs and associated fluids using biological tags and real-time polymerase chain reactions
DE102006026963B4 (de) * 2006-06-09 2008-05-08 DRK-Blutspendedienst Baden-Württemberg-Hessen gGmbH Verfahren zur Detektion von Bakterien in aus Blut abgeleiteten Proben mit Hilfe einer Realtime-PCR
FR2906260B1 (fr) * 2006-09-26 2012-11-30 Biotisa Procede d'analyse de substrats de decontamination.
ES2827773T3 (es) 2007-04-20 2021-05-24 Zenon Tech Partnership Aparato y procedimiento de biopelícula con soporte de membrana
WO2009046415A1 (en) * 2007-10-04 2009-04-09 The Trustees Of Columbia University In The City Of New York Systems and methods for achieving partial nitrification in a biological nitrogen removal reactor
EP2195414A4 (en) * 2007-10-04 2014-01-08 Univ Columbia SYSTEMS AND METHODS FOR EVALUATING OPERATING CONDITIONS IN A BIOREACTOR USING MONITORING OF GENE EXPRESSION AND ABUNDANCE
US8211639B2 (en) 2009-03-10 2012-07-03 Monsanto Technology Llc Imaging glyphosate in plant tissue
BRPI1010121B1 (pt) * 2009-08-28 2019-12-03 Kansai Coke & Chemicals Co Ltd método de tratamento biológico
CN103249844B (zh) 2010-10-14 2015-06-24 瑞昂尼克公司 目标核酸多标靶定量鉴定
US9612230B2 (en) * 2011-06-21 2017-04-04 Miura Co., Ltd. Water quality measuring device
KR102370290B1 (ko) 2013-02-22 2022-03-04 비엘 테크놀러지스 인크. 바이오필름 지지용 맴브레인 조립체
CN103409547B (zh) * 2013-08-20 2016-06-08 江苏省农业科学院 漂浮植物根系表面附着反硝化细菌中nirK基因的丰度实时检测方法及其应用
US10160679B2 (en) 2014-03-20 2018-12-25 Bl Technologies, Inc. Wastewater treatment with primary treatment and MBR or MABR-IFAS reactor
WO2016019572A1 (zh) * 2014-08-08 2016-02-11 浙江大学 基于mRNA响应的循环水养殖系统变速流控制方法及系统
CN104145874B (zh) * 2014-08-08 2016-02-03 浙江大学 基于mRNA响应的循环水养殖系统变速流控制方法及系统
CN105154564B (zh) * 2015-09-30 2018-06-19 上海海洋大学 基于环境dna技术对鱼类dna丰度估算的联合分析法
CN108060243B (zh) * 2017-12-21 2021-12-14 河海大学 一种高精度检测微生物与净污载体固着强度的方法
CN110241065B (zh) * 2019-05-18 2020-07-21 南京农业大学 一种利用土著PAHs降解菌群落去除农田土壤中USEPA PAHs的方法
JP2022000003A (ja) * 2020-06-19 2022-01-04 三菱重工業株式会社 生体の核酸抽出方法及び水処理システム
CN113362890B (zh) * 2021-04-28 2023-09-08 中国科学院生态环境研究中心 一种评价生物滤料降解有机物潜力的方法
CN113588336B (zh) * 2021-07-12 2022-08-05 南京市仪器仪表工业供销有限公司 水体采集系统及采样方法
CN115072884B (zh) * 2022-08-22 2022-12-02 广东省农业科学院农业资源与环境研究所 基于生物炭的污水中除草剂的净化处理方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4851131A (en) * 1987-12-31 1989-07-25 Monsanto Company Process for treating glyphosate process waste streams
US5213961A (en) * 1989-08-31 1993-05-25 Brigham And Women's Hospital Accurate quantitation of RNA and DNA by competetitive polymerase chain reaction
CA2033718A1 (en) 1990-01-19 1991-07-20 Ronald M. Atlas Process for detection of water-borne microbial pathogens and indicators of human fecal contamination in water samples and kits therefor
AU655197B2 (en) * 1990-06-25 1994-12-08 Monsanto Technology Llc Glyphosate tolerant plants
US5837501A (en) 1993-07-09 1998-11-17 Akzo Nobel N.V. Nucleic acid quantitation by co-amplification of target with multiple internal controls
US5792650A (en) 1993-09-03 1998-08-11 Ebara Corporation Method for treating waste water
US5705332A (en) 1994-04-25 1998-01-06 University Of Hawaii Detection and identification of Salmonella and Shigella
US5540840A (en) * 1995-06-02 1996-07-30 Monsanto Company Use of fluidized bed reactors for treatment of wastes containing organic nitrogen compounds
JP3770985B2 (ja) 1997-01-22 2006-04-26 大成建設株式会社 環境微生物からのdnaの直接抽出方法および細菌の検出方法
US6110661A (en) * 1997-05-01 2000-08-29 Eastman Chemical Company Bioluminescent reporter bacterium
NO974299D0 (no) 1997-09-18 1997-09-18 Forskningsparken I Aas As Metode for stabil merking av mikroorganismer
US5888740A (en) 1997-09-19 1999-03-30 Genaco Biomedical Products, Inc. Detection of aneuploidy and gene deletion by PCR-based gene- dose co-amplification of chromosome specific sequences with synthetic sequences with synthetic internal controls
US6106718A (en) 1998-07-01 2000-08-22 Biochem Technology, Inc. Enhanced denitrification process by monitoring and controlling carbonaceous nutrient addition

Also Published As

Publication number Publication date
CN1531601A (zh) 2004-09-22
US6849430B2 (en) 2005-02-01
WO2002085791A2 (en) 2002-10-31
EP1414750A2 (en) 2004-05-06
AR035832A1 (es) 2004-07-14
CN1268767C (zh) 2006-08-09
MXPA03009732A (es) 2004-01-29
EP1414750A4 (en) 2005-01-19
CA2444316A1 (en) 2002-10-31
WO2002085791A3 (en) 2004-03-04
AU2002257205B2 (en) 2006-09-21
US20030092020A1 (en) 2003-05-15

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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