BR0209095A - Monitoração baseada em pcr de sistemas de biotratamento de água residual - Google Patents
Monitoração baseada em pcr de sistemas de biotratamento de água residualInfo
- Publication number
- BR0209095A BR0209095A BR0209095-3A BR0209095A BR0209095A BR 0209095 A BR0209095 A BR 0209095A BR 0209095 A BR0209095 A BR 0209095A BR 0209095 A BR0209095 A BR 0209095A
- Authority
- BR
- Brazil
- Prior art keywords
- pcr
- gene
- indicator
- effector
- abundance
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/006—Regulation methods for biological treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
"MONITORAçãO BASEADA EM PCR DE SISTEMAS DE BIOTRATAMENTO DE áGUA RESIDUAL". A presente invenção refere-se à monitoração baseada em PCR de microorganismos em sistemas para o tratamento biológico de água residual. Em particular, medição de ambas, a abundância e expressão de genes indicadores/efetores ou combinações de genes, onde expressão de um gene "efetor" correlaciona-se com a atividade degradante de uma particular amostra microbial,e onde a abundância de um gene "indicador" correlaciona-se com a abundância de micróbios. Expressão de gene efetor é medida por RT-PCR quantitativa competitiva, e abundância de gene indicador é medida por PCR quantitativa competitiva. Os genes indicadores e efetores podem ser genes idênticos ou diferentes. Em uma realização, o gene indicador e efetor são seq³ências do gene glifosato oxidorredutase (gox) e as técnicas quantitativas empregadas são PCR quantitativa competitiva e RT-PCR quantitativa competitiva.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28584601P | 2001-04-23 | 2001-04-23 | |
PCT/US2002/012923 WO2002085791A2 (en) | 2001-04-23 | 2002-04-23 | Pcr-based monitoring in wastewater biotreatment systems |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0209095A true BR0209095A (pt) | 2004-07-13 |
Family
ID=23095951
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0209095-3A BR0209095A (pt) | 2001-04-23 | 2002-04-23 | Monitoração baseada em pcr de sistemas de biotratamento de água residual |
Country Status (9)
Country | Link |
---|---|
US (1) | US6849430B2 (pt) |
EP (1) | EP1414750A4 (pt) |
CN (1) | CN1268767C (pt) |
AR (1) | AR035832A1 (pt) |
AU (1) | AU2002257205B2 (pt) |
BR (1) | BR0209095A (pt) |
CA (1) | CA2444316A1 (pt) |
MX (1) | MXPA03009732A (pt) |
WO (1) | WO2002085791A2 (pt) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040161767A1 (en) * | 2002-06-28 | 2004-08-19 | Baldwin Brett R. | Detection and quantification of aromatic oxygenase genes by real-time PCR |
US7303676B2 (en) * | 2003-02-13 | 2007-12-04 | Zenon Technology Partnership | Supported biofilm apparatus and process |
US7175763B2 (en) * | 2003-02-13 | 2007-02-13 | Zenon Technology Partnership | Membrane supported biofilm process for autotrophic reduction |
US7300571B2 (en) * | 2003-02-13 | 2007-11-27 | Zenon Technology Partnership | Supported biofilm apparatus |
JP2006518661A (ja) * | 2003-02-13 | 2006-08-17 | ゼノン、エンバイロンメンタル、インコーポレーテッド | 支持されたバイオフィルム装置と方法 |
BRPI0709282B8 (pt) | 2006-03-29 | 2021-05-25 | Merial Ltd | vacina contra estreptococos |
GB2438216B (en) * | 2006-05-17 | 2008-11-19 | Schlumberger Holdings | Methods and systems for evaluation of hydrocarbon reservoirs and associated fluids using biological tags and real-time polymerase chain reactions |
DE102006026963B4 (de) * | 2006-06-09 | 2008-05-08 | DRK-Blutspendedienst Baden-Württemberg-Hessen gGmbH | Verfahren zur Detektion von Bakterien in aus Blut abgeleiteten Proben mit Hilfe einer Realtime-PCR |
FR2906260B1 (fr) * | 2006-09-26 | 2012-11-30 | Biotisa | Procede d'analyse de substrats de decontamination. |
ES2827773T3 (es) | 2007-04-20 | 2021-05-24 | Zenon Tech Partnership | Aparato y procedimiento de biopelícula con soporte de membrana |
WO2009046415A1 (en) * | 2007-10-04 | 2009-04-09 | The Trustees Of Columbia University In The City Of New York | Systems and methods for achieving partial nitrification in a biological nitrogen removal reactor |
EP2195414A4 (en) * | 2007-10-04 | 2014-01-08 | Univ Columbia | SYSTEMS AND METHODS FOR EVALUATING OPERATING CONDITIONS IN A BIOREACTOR USING MONITORING OF GENE EXPRESSION AND ABUNDANCE |
US8211639B2 (en) | 2009-03-10 | 2012-07-03 | Monsanto Technology Llc | Imaging glyphosate in plant tissue |
BRPI1010121B1 (pt) * | 2009-08-28 | 2019-12-03 | Kansai Coke & Chemicals Co Ltd | método de tratamento biológico |
CN103249844B (zh) | 2010-10-14 | 2015-06-24 | 瑞昂尼克公司 | 目标核酸多标靶定量鉴定 |
US9612230B2 (en) * | 2011-06-21 | 2017-04-04 | Miura Co., Ltd. | Water quality measuring device |
KR102370290B1 (ko) | 2013-02-22 | 2022-03-04 | 비엘 테크놀러지스 인크. | 바이오필름 지지용 맴브레인 조립체 |
CN103409547B (zh) * | 2013-08-20 | 2016-06-08 | 江苏省农业科学院 | 漂浮植物根系表面附着反硝化细菌中nirK基因的丰度实时检测方法及其应用 |
US10160679B2 (en) | 2014-03-20 | 2018-12-25 | Bl Technologies, Inc. | Wastewater treatment with primary treatment and MBR or MABR-IFAS reactor |
WO2016019572A1 (zh) * | 2014-08-08 | 2016-02-11 | 浙江大学 | 基于mRNA响应的循环水养殖系统变速流控制方法及系统 |
CN104145874B (zh) * | 2014-08-08 | 2016-02-03 | 浙江大学 | 基于mRNA响应的循环水养殖系统变速流控制方法及系统 |
CN105154564B (zh) * | 2015-09-30 | 2018-06-19 | 上海海洋大学 | 基于环境dna技术对鱼类dna丰度估算的联合分析法 |
CN108060243B (zh) * | 2017-12-21 | 2021-12-14 | 河海大学 | 一种高精度检测微生物与净污载体固着强度的方法 |
CN110241065B (zh) * | 2019-05-18 | 2020-07-21 | 南京农业大学 | 一种利用土著PAHs降解菌群落去除农田土壤中USEPA PAHs的方法 |
JP2022000003A (ja) * | 2020-06-19 | 2022-01-04 | 三菱重工業株式会社 | 生体の核酸抽出方法及び水処理システム |
CN113362890B (zh) * | 2021-04-28 | 2023-09-08 | 中国科学院生态环境研究中心 | 一种评价生物滤料降解有机物潜力的方法 |
CN113588336B (zh) * | 2021-07-12 | 2022-08-05 | 南京市仪器仪表工业供销有限公司 | 水体采集系统及采样方法 |
CN115072884B (zh) * | 2022-08-22 | 2022-12-02 | 广东省农业科学院农业资源与环境研究所 | 基于生物炭的污水中除草剂的净化处理方法 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4851131A (en) * | 1987-12-31 | 1989-07-25 | Monsanto Company | Process for treating glyphosate process waste streams |
US5213961A (en) * | 1989-08-31 | 1993-05-25 | Brigham And Women's Hospital | Accurate quantitation of RNA and DNA by competetitive polymerase chain reaction |
CA2033718A1 (en) | 1990-01-19 | 1991-07-20 | Ronald M. Atlas | Process for detection of water-borne microbial pathogens and indicators of human fecal contamination in water samples and kits therefor |
AU655197B2 (en) * | 1990-06-25 | 1994-12-08 | Monsanto Technology Llc | Glyphosate tolerant plants |
US5837501A (en) | 1993-07-09 | 1998-11-17 | Akzo Nobel N.V. | Nucleic acid quantitation by co-amplification of target with multiple internal controls |
US5792650A (en) | 1993-09-03 | 1998-08-11 | Ebara Corporation | Method for treating waste water |
US5705332A (en) | 1994-04-25 | 1998-01-06 | University Of Hawaii | Detection and identification of Salmonella and Shigella |
US5540840A (en) * | 1995-06-02 | 1996-07-30 | Monsanto Company | Use of fluidized bed reactors for treatment of wastes containing organic nitrogen compounds |
JP3770985B2 (ja) | 1997-01-22 | 2006-04-26 | 大成建設株式会社 | 環境微生物からのdnaの直接抽出方法および細菌の検出方法 |
US6110661A (en) * | 1997-05-01 | 2000-08-29 | Eastman Chemical Company | Bioluminescent reporter bacterium |
NO974299D0 (no) | 1997-09-18 | 1997-09-18 | Forskningsparken I Aas As | Metode for stabil merking av mikroorganismer |
US5888740A (en) | 1997-09-19 | 1999-03-30 | Genaco Biomedical Products, Inc. | Detection of aneuploidy and gene deletion by PCR-based gene- dose co-amplification of chromosome specific sequences with synthetic sequences with synthetic internal controls |
US6106718A (en) | 1998-07-01 | 2000-08-22 | Biochem Technology, Inc. | Enhanced denitrification process by monitoring and controlling carbonaceous nutrient addition |
-
2002
- 2002-04-23 AU AU2002257205A patent/AU2002257205B2/en not_active Ceased
- 2002-04-23 US US10/128,781 patent/US6849430B2/en not_active Expired - Lifetime
- 2002-04-23 BR BR0209095-3A patent/BR0209095A/pt not_active IP Right Cessation
- 2002-04-23 AR ARP020101462A patent/AR035832A1/es not_active Application Discontinuation
- 2002-04-23 CA CA002444316A patent/CA2444316A1/en not_active Abandoned
- 2002-04-23 CN CNB02812586XA patent/CN1268767C/zh not_active Expired - Fee Related
- 2002-04-23 EP EP02726797A patent/EP1414750A4/en not_active Withdrawn
- 2002-04-23 WO PCT/US2002/012923 patent/WO2002085791A2/en not_active Application Discontinuation
- 2002-04-23 MX MXPA03009732A patent/MXPA03009732A/es unknown
Also Published As
Publication number | Publication date |
---|---|
CN1531601A (zh) | 2004-09-22 |
US6849430B2 (en) | 2005-02-01 |
WO2002085791A2 (en) | 2002-10-31 |
EP1414750A2 (en) | 2004-05-06 |
AR035832A1 (es) | 2004-07-14 |
CN1268767C (zh) | 2006-08-09 |
MXPA03009732A (es) | 2004-01-29 |
EP1414750A4 (en) | 2005-01-19 |
CA2444316A1 (en) | 2002-10-31 |
WO2002085791A3 (en) | 2004-03-04 |
AU2002257205B2 (en) | 2006-09-21 |
US20030092020A1 (en) | 2003-05-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE 6A., 7A. E 8A. ANUIDADES. |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2060 DE 29/06/2010. |