BR0011765A - Vetores para aperfeiçoar a clonagem e a expressão em plasmìdios com baixo número de cópias - Google Patents

Vetores para aperfeiçoar a clonagem e a expressão em plasmìdios com baixo número de cópias

Info

Publication number
BR0011765A
BR0011765A BR0011765-0A BR0011765A BR0011765A BR 0011765 A BR0011765 A BR 0011765A BR 0011765 A BR0011765 A BR 0011765A BR 0011765 A BR0011765 A BR 0011765A
Authority
BR
Brazil
Prior art keywords
plasmids
bac
cloning
expression
copies
Prior art date
Application number
BR0011765-0A
Other languages
English (en)
Inventor
Trudy Grossman
Ian Macneil
Paul August
Original Assignee
Aventis Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Inc filed Critical Aventis Pharma Inc
Publication of BR0011765A publication Critical patent/BR0011765A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Patente de Invenção: "VETORES PARA APERFEIçOAR A CLONAGEM E A EXPRESSãO EM PLASMìDIOS COM BAIXO NúMERO DE CóPIAS". Métodos que facilitam 1) a clonagem de insertos grandes em plasmídios de BAC, 2) o isolamento de grandes quantidades de DNA de BAC (por aumento do número de cópias dos plasmídios), e 3) o aumento da expressão heteróloga a partir de insertos de plasmídios de BAC (por aumento do número de cópias dos plasmídios e/ou introdução de promotores no inserto). é proporcionado um vetor para aumentar o número de cópias dos plasmídios, compreendendo um elemento transponível contendo um ponto de origem da replicação com número de cópias moderado ou alto, capaz de transposição in vitro para um plasmídio-alvo. O plasmídio-alvo é um plasmídio de cópia única ou baixa, por exemplo, um vetor de BAC, que é útil para a clonagem de grandes pedaços de DNA. O plasmídio com transposon pode conter qualquer ponto de origem da replicação com cópia moderada ou alta que seja compatível com um hospedeiro bacteriano, tal como E coli. Assim, um ori ilustrativo é o ori colE1 a partir de pBR322.
BR0011765-0A 1999-06-18 2000-06-16 Vetores para aperfeiçoar a clonagem e a expressão em plasmìdios com baixo número de cópias BR0011765A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14028799P 1999-06-18 1999-06-18
PCT/US2000/016767 WO2000078977A1 (en) 1999-06-18 2000-06-16 Novel vectors for improving cloning and expression in low copy number plasmids

Publications (1)

Publication Number Publication Date
BR0011765A true BR0011765A (pt) 2002-03-05

Family

ID=22490577

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0011765-0A BR0011765A (pt) 1999-06-18 2000-06-16 Vetores para aperfeiçoar a clonagem e a expressão em plasmìdios com baixo número de cópias

Country Status (12)

Country Link
EP (1) EP1196613A1 (pt)
JP (1) JP2003503029A (pt)
KR (1) KR20020011139A (pt)
AU (1) AU5496600A (pt)
BR (1) BR0011765A (pt)
CA (1) CA2375821A1 (pt)
IL (1) IL147125A0 (pt)
MX (1) MXPA01012406A (pt)
NO (1) NO20016153L (pt)
NZ (1) NZ515502A (pt)
WO (1) WO2000078977A1 (pt)
ZA (1) ZA200109441B (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6864087B2 (en) * 2001-07-31 2005-03-08 Wisconsin Alumni Research Foundation Method for converting single-copy BAC vectors to conditional high-copy pBAC/oriHC vectors
EP1448773A4 (en) * 2001-11-15 2006-07-05 Aventis Pharma Inc GENETICALLY MODIFIED BACTERIAL STRAINS AND NOVEL VECTORS FOR USE IN THE EXPRESSION AND DETERMINATION OF NATURAL PRODUCTS
EP1386966A1 (en) * 2002-07-24 2004-02-04 Libragen Method for the expression of unknown environmental DNA into adapted host cells
WO2016080424A1 (ja) 2014-11-18 2016-05-26 国立研究開発法人 科学技術振興機構 環状ｄｎａの増幅方法
US11155822B2 (en) 2015-05-14 2021-10-26 Universidad De Los Andes Transposon that promotes functional DNA expression in episomal DNAs and method to enhance DNA transcription during functional analysis of metagenomic libraries
BR112019017657A2 (pt) * 2017-02-28 2020-04-07 Oriciro Genomics Inc método para replicação ou amplificação de dna circular

Also Published As

Publication number Publication date
JP2003503029A (ja) 2003-01-28
ZA200109441B (en) 2003-04-30
AU5496600A (en) 2001-01-09
KR20020011139A (ko) 2002-02-07
EP1196613A1 (en) 2002-04-17
WO2000078977A1 (en) 2000-12-28
NO20016153L (no) 2002-02-15
CA2375821A1 (en) 2000-12-28
NO20016153D0 (no) 2001-12-17
NZ515502A (en) 2004-08-27
MXPA01012406A (es) 2002-06-04
IL147125A0 (en) 2002-08-14

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A, 7A E 8A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1962 DE 12/08/2008.