BG4273U1 - Synbiotic composition - Google Patents

Abstract

The utility model relates to a synbiotic composition comprising a mixture of lyophilized live cell cultures of the bacterial strains Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus and at least one strain of the genus Bifidobacterium, as well as inulin, dried sheep's milk and/or lyophilized sheep's colostrum and/or a mixture thereof, lyophilized snail extract, vitamins, flavours and additives, in the described amounts by weight % according to this utility model.

Description

Field of technique

The utility model relates to a synbiotic composition that will find use as a prophylactic and therapeutic preparation for maintaining and improving the general health of the host, as well as for the prevention and/or treatment of diseases of the gastrointestinal tract intended for humans.

Prior art

Lactic acid bacteria are a diverse group of gram-positive, non-sporulating bacteria, anaerobes or facultative anaerobes, requiring a specific nutrient medium, and whose end metabolic product is lactic acid. Nowadays, lactic acid bacteria are microorganisms of industrial importance due to their fermentation activity as well as their health and nutritional benefits. Lactic acid bacteria, which occupy important niches in the gastrointestinal tract, are seen as offering numerous probiotic benefits for general health and well-being. These advantages include a positive influence on the natural microflora, competitive exclusion of pathogens, stimulation and modulation of mucosal immunity. In this regard, it is worth paying special attention to the fact that the health benefits due to probiotic bacteria are strain specific and depend on the characteristics of individual strains (Shah NP. Functional cultures and health benefits. Int. Dairy J. 2007 ; 17: 1262-1277).

International patent application WO 2008141240 discloses probiotic foods and probiotic supplement compositions with immunological activity. The compositions contain viable microbial cells of at least one probiotic in combination with at least one natural polysaccharide (prebiotic) and other additives, in the form of capsules or tablets.

From patent publication BG 67244 B1 is known an immunomodulating synbiotic composition containing an active formula based on a probiotic from living cells of a biologically pure culture of a bacterial strain selected from the group of Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus helveticus, Lactobacillus acidophilus, Lactobacillus rhamnosus and Lactobacillus fermentum or their combinations, prebiotic arabinogalactan from the bark of Siberian larch (Larix gmelinii) and a milk product in the form of colostrum in the amounts described in mg. The compositions of BG 67244 B1 are intended for obtaining a food supplement and/or a pharmaceutical product for the prevention and/or the therapy of inflammatory and immune diseases of the gastrointestinal tract.

Technical nature of the utility model

An object of the present utility model is to create a new form of synbiotic composition that helps maintain and improve the general health of the host and serves to prevent and/or treat diseases of the gastrointestinal tract.

The task of the present utility model has been solved by creating a synbiotic composition including a prebiotic, a dairy product and a probiotic. The probiotic includes a mixture of living cells of bacterial strains of Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus acidophilus.

According to the present utility model, the mixture of living cells also contains at least one strain representative of the genus Bifidobacterium. The living cells of each of the bacterial strains Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus and Bifidobacterium are lyophilized cultures of these strains. Each bacterial strain in the live cell mixture had an activity in the range of 10 9 to 10 ¹¹ CFU/g of the mixture. The prebiotic in the synbiotic composition is inulin with a degree of polymerization of up to 10 units, which is preferably chosen to be from 5 to 8 units. The dairy product is dry sheep's milk and/or lyophilized sheep's colostrum and/or a mixture thereof. The components of the synbiotic composition are in an amount expressed in weight. %, as follows:

mixture of living cells - from 30 to 35;

inulin - from 28 to 33;

dairy product - from 30 to 35, such as up to 100 wt. % at least one component is selected from the group of vitamins, flavors and/or additives.

In a preferred embodiment of the synbiotic composition, according to the useful model, the living cells of the genus Bifidobacterium are from at least one strain selected from the group of Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium animalis, Bifidobacterium infantis or a combination thereof.

In another preferred embodiment of the synbiotic composition, according to the utility model, the bacterial strains in the living cell mixture are in an amount by weight. %, as follows:

Lactobacillus delbrueckii ssp. bulgaricus - from 45 to 55;

Lactobacillus acidophilus - from 25 to 30;

Bifidobacterium - from 20 to 25.

In variant embodiments of the synbiotic composition, according to the present utility model, the sheep milk powder and/or lyophilized sheep colostrum is from a domestic sheep selected from the Lakon, Assaf and/or Avasi breeds.

In a preferred embodiment, according to the present utility model, the synbiotic composition further comprises a lyophilized snail extract, which may be snail mucus in an amount of 40 mg to 60 mg, which is preferably chosen to be 50 mg.

In variant embodiments of the synbiotic composition according to the present utility model, the total amount of vitamins may be from 0.04 to 0.08 mg, preferably in the final product the amount is 0.05 mg, and as the preferred vitamin, vitamin D3 is selected. The flavors are desirably in the amount of 40 mg to 60 mg, which is preferably 50 mg in the final product, and the preferred flavor is bourbon vanilla (Vanilia planifolia). The amount of additives is from 15 mg to 25 mg, preferably in the final product the amount is 20 mg, and magnesium stearate is chosen as the preferred additive.

The synbiotic composition has exceptional complexity and high activity. All the combined ingredients of the product have a similar effect and complement each other, bringing new and additional effects of impact on the human organism. The synbiotic composition has a high content of lyophilized cultures of the bacterial strains of lactic acid bacteria, which retain their viability and high titer during long-term storage.

Examples of implementation of the utility model

The synbiotic composition, according to the present utility model, is presented with the following exemplary embodiments which will achieve the desired effects, by the disclosed features in the description and claims, and which fall within the scope of the present utility model defined by the claims, as follows:

Example 1.

A synbiotic composition comprising a mixture of lyophilized live cell cultures of the bacterial strains Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus and at least one strain of the genus Bifidobacterium, preferably e.g. Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium animalis, Bifidobacterium infantis or other suitable genera, as well as inulin, lyophilized sheep colostrum, lyophilized snail extract (mucus) , vitamins, flavors and supplements in the form of sachets with the following content:

Mixture of living cells (lyophilisate of bacterial strains) - 1300 mg

Inulin - 930 mg

Lyophilized sheep colostrum - 1000 mg

Lyophilized extract of snails (mucus) - 50 mg

Vitamin D3 - 0.05 mg (50 μg)

Bourbon vanilla flavor - 50 mg

Additive magnesium stearate - 20 mg

Example 2.

A synbiotic composition comprising a mixture of lyophilized live cell cultures of the bacterial strains Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus and at least one strain of the genus Bifidobacterium, preferably e.g. Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium animalis, Bifidobacterium infantis or other suitable genera, as well as inulin, sheep's milk powder, lyophilized snail extract (mucilage) , vitamins, flavors and supplements in the form of sachets with the following content:

Mixture of living cells (lyophilisate of bacterial strains) - 1300 mg

Inulin - 930 mg

Dry sheep's milk - 1000 mg

Lyophilized extract of snails (mucus) - 50 mg

Vitamin D ₃ - 0.05 mg (50 μg)

Bourbon vanilla flavor - 50 mg

Additive magnesium stearate - 20 mg

Example 3.

A synbiotic composition comprising a mixture of lyophilized live cell cultures of the bacterial strains Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus and at least one strain of the genus

Bifidobacterium, preferably e.g. Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium animalis, Bifidobacterium infantis or other suitable genera, as well as inulin, lyophilized sheep colostrum, vitamins, flavorings and supplements in sachet form with the following contents:

Mixture of living cells (lyophilisate of bacterial strains) - 1300 mg

Inulin - 930 mg

Lyophilized sheep colostrum - 1000 mg

Vitamin D ₃ - 0.05 mg (50 μg)

Bourbon vanilla flavor - 50 mg

Additive magnesium stearate - 20 mg

Example 4.

A synbiotic composition comprising a mixture of lyophilized live cell cultures of the bacterial strains Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus and at least one strain of the genus Bifidobacterium, preferably for example Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium animalis, Bifidobacterium infantis or other suitable genera, as well as inulin, sheep's milk powder, vitamins, flavors and additives in the form per sachet with the following contents:

Mixture of living cells (lyophilisate of bacterial strains) - 1300 mg

Inulin - 930 mg

Dry sheep's milk - 1000 mg

Vitamin D3 - 0.05 mg (50 μg)

Bourbon vanilla flavor - 50 mg

Additive magnesium stearate - 20 mg

Example 5.

A lyophilizate (mixture of live cells) of bacterial strains comprising two strains of lactic acid bacteria Lactobacillus delbrueckii ssp. bulgaricus, one strain of lactic acid bacteria Lactobacillus acidophilus and one strain of probiotic bacteria and at least one strain of the genus Bifidobacterium, preferably for example Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium animalis, Bifidobacterium infantis or other suitable genera with the following content:

the strain Lactobacillus delbrueckii ssp. bulgaricus - 650 mg Lactobacillus acidophilus strain - 350 mg

At least 1 strain representative of the genus Bifidobacterium - 300 mg

Although the present utility model has been described in connection with the preferred embodiment, it should be noted that other alternative embodiments are possible which will be apparent to a person of ordinary skill and knowledge and which will achieve the desired effects through the features disclosed herein. description that do not go beyond the scope of the present utility model as defined by the claims.

Application of the utility model

The synbiotic composition is a useful and complex product, which includes a mixture of lyophilized living cells of the bacterial strains Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus and at least one strain of the genus Bifidobacterium, dry sheep colostrum and/or dry sheep milk, inulin with a degree of polymerization of less than 10 units (preferably between 5 and 8), lyophilized snail extract (mucus) and vitamin D3, natural flavors and aromas. All the combined ingredients of the product have a similar effect, but at the same time complement each other and bring new and additional effects on the human body.

Each of the bacterial strains of lactic acid bacteria Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus acidophilus was cultured and lyophilized separately, the culture being carried out in a bioreactor with a species-appropriate milk-based medium. The finished lyophilisate is mixed and packaged in two-component aluminum foil. It is stored at a temperature below -18°C for up to two years. For the preparation of the synbiotic composition, the necessary amounts of each ingredient are weighed as a percentage of the total amount of mixture required to prepare the batch. All components are sieved through a sieve with an opening size of 0.5 mm. All components, with the exception of magnesium stearate, are fed to a mixer where they are mixed for 30 min. The magnesium stearate was added to the mixture and mixed again for an additional 10 min. The finished mixture is packaged in a suitable form such as tablets, capsules, granules, liquid bacterial suspensions, paste, powder or preferably sachets. The synbiotic composition can be stored in refrigerated conditions for 2 to 3 years, and at room temperature for 12 to 24 months.

The complex nature of the product comes from specially selected strains of probiotic bacteria Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus and Bifidobacterium, which support and regulate the bacterial balance of the microflora of the gastrointestinal tract in dysbacteriosis, diarrhea, gastrointestinal infections, after treatment with antibiotics, and also in the prevention of complications due to taking antibiotics, in disturbed functions of the digestive system (such as bloating and heartburn, flatulence and constipation, damage to the intestinal mucosa, lactase deficiency). With regular use, the product normalizes the immune system, reduces allergic reactions, suppresses putrefactive bacteria, reduces increased gas formation in the intestines; lowers levels of "bad" cholesterol in children and adults.

The genus Bifidobacterium includes a variety of Gram-positive, non-motile anaerobic bacteria. They are endosymbiotic inhabitants of the gastrointestinal tract and vagina of mammals, including humans. Strains of the genus Bifidobacterium are often used as probiotic bacteria because they are known for their variety of bile salt resistance mechanisms, which is important because the beneficial effects of probiotic bacteria must be generated in the presence of bile salt. Strains of Bifidobacteria, including: Bifidobacterium infantis, B. adolescentis, B. animalis subsp. animalis, B. animalis subsp. lactis, B. bifidum, B. longum, B. breve are most commonly used as probiotics. Bifidobacterium species in combination with other probiotics have been shown to treat constipation, traveler's diarrhea, antibiotic-associated diarrhea, maintain remission of inflammatory bowel disease and moderate ulcerative colitis, prevent as well as treat neonatal necrotizing enterocolitis, reduce radiation diarrhea, reducing the risk of developing eczema, food allergies, cholesterol-lowering abilities.

Due to the presence of lyophilized extract of snails (mucus), the product has a beneficial effect on the musculoskeletal system, strengthening joint cartilage and tendons, stimulating the immune system and having an antioxidant and toning effect. It will favorably affect the mobility of the joints and the general functional state of the body. Snail extract contains regenerative components, such as allantoin, collagen and elastin, which are quite close to those in human skin. Thus, the formation of new elastin, collagen and pigment cells is stimulated. Snail extract has specific antimicrobial properties. Destroys bacteria - Escherichia coli, Staphylococcus aureus and others, which often cause inflammation. Due to the presence of a number of peptides exhibiting antibacterial properties, snail extract can be successfully used in combination with antibiotic therapy against infections caused by Helicobacter pylori. It acts as a natural antibiotic, and unlike drugs, resistance to it does not develop. It has a beneficial effect on the digestive tract and stimulates the immune system. Thus, the proposed product can be successfully used for gastrointestinal problems.

The presence of the prebiotic inulin with a low degree of polymerization of up to 10 units, which is preferably between 5 and 8 units, in the product supports the development of beneficial bacteria of the genus Bifidobacterium in the large intestine, favors the reduction of blood sugar levels and the increase of physiological efficiency of insulin; prevents the development of diabetic retinopathies, reduces the likelihood of the development of cardiovascular diseases; normalizes carbohydrate and fat metabolism in obesity and helps to reduce excess body mass, naturally reduces the feeling of hunger.

The contained colostrum (from the elite "Lacon" sheep breed) in the product supplies valuable elements not only to the growing organism, but also to everyone else. Proven to soothe the gastric mucosa in gastritis and ulcers, stimulate recovery in herpes zoster, flu. It supports the functions of the internal organs and in particular those on which the condition of patients with diabetes, Hashimoto's depends. It also has a therapeutic effect on psoriasis, neurodermatitis and acne. It is an indisputable fact that colostrum is most valuable for human immunity. It is rich in antibodies that help the immune system to fight various allergens, "bad" bacteria, fungi and viruses. In this way, it suppresses inflammatory processes and reduces the intensity of symptoms. Cholesterol stimulates the secretion of the immune globulin IgA, the levels of which are reduced in people suffering from irritable bowel syndrome, irritable bowel syndrome, Crohn's disease, ulcerative colitis, candida, autism. Colostrum strengthens the protective functions of the intestinal tract and balances the immune response by reducing the levels of cytokines (proteins that inform immune cells of inflammation). The growth and immune factors in colostrum provide reliable assistance in the fight against cancer. It is believed to be able to prevent the growth of these cells and limit their spread in the body. The presence of colostrum from an elite breed of sheep in the product together with other ingredients will have a beneficial effect on:

• rheumatoid arthritis - can reduce inflammation;

• osteoporosis and osteoarthritis - helps build bone density;

• transplant - immunoglobulins could help reduce fungal and bacterial infections;

• cytokines in colostrum help cancer because they have powerful interleukins;

• the AIDS virus - reduction of infectious pathogens that cause the conditions associated with the virus;

• obesity - can help the body digest food better;

• restore the skin and reduce the effects of aging.

Claims (1)

A synbiotic composition comprising a probiotic, a prebiotic and a dairy product, wherein the probiotic comprises a mixture of living cells of bacterial strains of Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus acidophilus, characterized in that the mixture of living cells also contains at least one strain representative of the genus Bifidobacterium, wherein the living cells of the bacterial strains Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, and Bifidobacterium are lyophilized cultures of each of these strains, each bacterial strain in the live cell mixture having activity ranging from 109 to 1011 CFU/g of the mixture, and the prebiotic is inulin with a degree of polymerization of up to 10 units, while the dairy product is dry sheep's milk and/or lyophilized sheep's colostrum and/or a mixture thereof, where the components of the composition are in an amount expressed in weight. %, as follows: a mixture of living cells - from 30 to 35; inulin - from 28 to 33; dairy product - from 30 to 35, such as up to 100 wt. % at least one component is selected from the group of vitamins, flavors and/or additives