AU8592298A - Rapid subcloning using site-specific recombination - Google Patents

Rapid subcloning using site-specific recombination Download PDF

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Publication number
AU8592298A
AU8592298A AU85922/98A AU8592298A AU8592298A AU 8592298 A AU8592298 A AU 8592298A AU 85922/98 A AU85922/98 A AU 85922/98A AU 8592298 A AU8592298 A AU 8592298A AU 8592298 A AU8592298 A AU 8592298A
Authority
AU
Australia
Prior art keywords
nucleic acid
site
sequence
gene
construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU85922/98A
Other languages
English (en)
Inventor
Stephen J Elledge
Qinghua Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baylor College of Medicine
Original Assignee
Baylor College of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/122,384 external-priority patent/US6828093B1/en
Application filed by Baylor College of Medicine filed Critical Baylor College of Medicine
Publication of AU8592298A publication Critical patent/AU8592298A/en
Priority to AU2004201968A priority Critical patent/AU2004201968A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU85922/98A 1998-07-24 1998-07-27 Rapid subcloning using site-specific recombination Abandoned AU8592298A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2004201968A AU2004201968A1 (en) 1998-07-24 2004-05-10 Rapid subcloning using site-specific recombination

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09122384 1998-07-24
US09/122,384 US6828093B1 (en) 1997-02-28 1998-07-24 Rapid subcloning using site-specific recombination
PCT/US1998/015481 WO2000005355A1 (fr) 1998-07-24 1998-07-27 Sous-clonage rapide faisant appel a une recombinaison specifique a un site

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2004201968A Division AU2004201968A1 (en) 1998-07-24 2004-05-10 Rapid subcloning using site-specific recombination

Publications (1)

Publication Number Publication Date
AU8592298A true AU8592298A (en) 2000-02-14

Family

ID=22402395

Family Applications (1)

Application Number Title Priority Date Filing Date
AU85922/98A Abandoned AU8592298A (en) 1998-07-24 1998-07-27 Rapid subcloning using site-specific recombination

Country Status (5)

Country Link
EP (1) EP1100885A4 (fr)
JP (1) JP2002522013A (fr)
AU (1) AU8592298A (fr)
CA (1) CA2336520A1 (fr)
WO (1) WO2000005355A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1194540A1 (fr) 1999-07-14 2002-04-10 Clontech Laboratories Inc. Procedes faisant intervenir la recombinase pour la production de vecteurs d'expression et compositions permettant d'atteindre ce resultat
US7517688B2 (en) 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
CA2401632A1 (fr) 2000-02-25 2001-08-30 Stratagene Procede de ligature d'acides nucleiques et de clonage moleculaire
ES2350248T3 (es) * 2001-10-01 2011-01-20 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Procedimiento de producción de bibliotecas de proteínas y de selección de proteínas a partir de las mismas.
FR2831185A1 (fr) * 2001-10-19 2003-04-25 Genoplante Valor Procede et kit de conversion de vecteurs hybrides de clonage en phase solide et procede de clonage de sequences nucleotidiques d'interet en faisant application
US7160702B2 (en) * 2002-07-26 2007-01-09 Shuwei Yang Methods and nucleic acid vectors for rapid expression and screening of CDNA clones
ATE443128T1 (de) 2004-10-22 2009-10-15 Novozymes As Stabile genomische integration mehrerer polynukleotidkopien
AU2022334711A1 (en) * 2021-08-23 2024-04-04 Bioverativ Therapeutics Inc. Baculovirus expression system

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1293460C (fr) * 1985-10-07 1991-12-24 Brian Lee Sauer Recombinaison a des sites specifiques de l'adn dans les levures
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
JP3253071B2 (ja) * 1989-12-22 2002-02-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 植物細胞の中のｄｎａの部位特異的組み換え
WO1993015191A1 (fr) * 1992-01-24 1993-08-05 Life Technologies, Inc. Modulation d'activites enzymatiques lors du clonage in vivo d'adn
EG23907A (en) * 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5801030A (en) * 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US7135608B1 (en) * 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
CN100342008C (zh) * 1997-10-24 2007-10-10 茵维特罗根公司 利用具重组位点的核酸进行重组克隆

Also Published As

Publication number Publication date
JP2002522013A (ja) 2002-07-23
EP1100885A1 (fr) 2001-05-23
EP1100885A4 (fr) 2001-12-12
WO2000005355A1 (fr) 2000-02-03
CA2336520A1 (fr) 2000-02-03

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Legal Events

Date Code Title Description
MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted