EP1100885A4 - Sous-clonage rapide faisant appel a une recombinaison specifique a un site - Google Patents

Sous-clonage rapide faisant appel a une recombinaison specifique a un site

Info

Publication number
EP1100885A4
EP1100885A4 EP98937141A EP98937141A EP1100885A4 EP 1100885 A4 EP1100885 A4 EP 1100885A4 EP 98937141 A EP98937141 A EP 98937141A EP 98937141 A EP98937141 A EP 98937141A EP 1100885 A4 EP1100885 A4 EP 1100885A4
Authority
EP
European Patent Office
Prior art keywords
site
specific recombination
rapid subcloning
subcloning
rapid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP98937141A
Other languages
German (de)
English (en)
Other versions
EP1100885A1 (fr
Inventor
Stephen J Dr Elledge
Qinghua Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baylor College of Medicine
Original Assignee
Baylor College of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/122,384 external-priority patent/US6828093B1/en
Application filed by Baylor College of Medicine filed Critical Baylor College of Medicine
Publication of EP1100885A1 publication Critical patent/EP1100885A1/fr
Publication of EP1100885A4 publication Critical patent/EP1100885A4/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP98937141A 1998-07-24 1998-07-27 Sous-clonage rapide faisant appel a une recombinaison specifique a un site Ceased EP1100885A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US122384 1980-02-19
US09/122,384 US6828093B1 (en) 1997-02-28 1998-07-24 Rapid subcloning using site-specific recombination
PCT/US1998/015481 WO2000005355A1 (fr) 1998-07-24 1998-07-27 Sous-clonage rapide faisant appel a une recombinaison specifique a un site

Publications (2)

Publication Number Publication Date
EP1100885A1 EP1100885A1 (fr) 2001-05-23
EP1100885A4 true EP1100885A4 (fr) 2001-12-12

Family

ID=22402395

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98937141A Ceased EP1100885A4 (fr) 1998-07-24 1998-07-27 Sous-clonage rapide faisant appel a une recombinaison specifique a un site

Country Status (5)

Country Link
EP (1) EP1100885A4 (fr)
JP (1) JP2002522013A (fr)
AU (1) AU8592298A (fr)
CA (1) CA2336520A1 (fr)
WO (1) WO2000005355A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6410317B1 (en) 1999-07-14 2002-06-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
US7517688B2 (en) 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
EP1504119A4 (fr) 2000-02-25 2005-02-09 Stratagene California Procede de ligature d'acides nucleiques et de clonage moleculaire
DE50115587D1 (de) 2001-10-01 2010-09-16 Deutsches Krebsforsch Verfahren zur Herstellung von Protein-Bibliotheken und zur Selektion von Proteinen daraus
FR2831185A1 (fr) * 2001-10-19 2003-04-25 Genoplante Valor Procede et kit de conversion de vecteurs hybrides de clonage en phase solide et procede de clonage de sequences nucleotidiques d'interet en faisant application
JP2006506972A (ja) * 2002-07-26 2006-03-02 ジェネコポエイア・インコーポレイテッド ｃＤＮＡクローンを迅速に発現させてスクリーニングするための方法および核酸ベクター
DK1805296T3 (da) * 2004-10-22 2010-01-18 Novozymes As Stabil genomisk integrering af multipie polynukleotidkopier
CA3229668A1 (fr) * 2021-08-23 2023-03-02 Bioverativ Therapeutics Inc. Systeme d'expression de baculovirus

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0220009A2 (fr) * 1985-10-07 1987-04-29 E.I. Du Pont De Nemours And Company Recombinaison à site spécifique de l'ADN dans la levure
WO1991009957A1 (fr) * 1989-12-22 1991-07-11 E.I. Du Pont De Nemours And Company Recombinaison specifique au site d'adn dans des cellules vegetales
WO1993015191A1 (fr) * 1992-01-24 1993-08-05 Life Technologies, Inc. Modulation d'activites enzymatiques lors du clonage in vivo d'adn
WO1996004393A2 (fr) * 1994-08-01 1996-02-15 Delta And Pine Land Company Regulation de l'expression d'un gene de plante
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
WO1999010488A1 (fr) * 1997-08-28 1999-03-04 The Salk Institute For Biological Studies Recombinaison specifique de site chez les eucaryotes et produits de recombinaison utilises a cet effet
WO1999021977A1 (fr) * 1997-10-24 1999-05-06 Life Technologies, Inc. Clonage recombinatoire au moyen d'acides nucleiques possedant des sites de recombinaison

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5801030A (en) * 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0220009A2 (fr) * 1985-10-07 1987-04-29 E.I. Du Pont De Nemours And Company Recombinaison à site spécifique de l'ADN dans la levure
WO1991009957A1 (fr) * 1989-12-22 1991-07-11 E.I. Du Pont De Nemours And Company Recombinaison specifique au site d'adn dans des cellules vegetales
WO1993015191A1 (fr) * 1992-01-24 1993-08-05 Life Technologies, Inc. Modulation d'activites enzymatiques lors du clonage in vivo d'adn
WO1996004393A2 (fr) * 1994-08-01 1996-02-15 Delta And Pine Land Company Regulation de l'expression d'un gene de plante
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
WO1999010488A1 (fr) * 1997-08-28 1999-03-04 The Salk Institute For Biological Studies Recombinaison specifique de site chez les eucaryotes et produits de recombinaison utilises a cet effet
WO1999021977A1 (fr) * 1997-10-24 1999-05-06 Life Technologies, Inc. Clonage recombinatoire au moyen d'acides nucleiques possedant des sites de recombinaison

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
AKAGI K ET AL: "CRE-MEDIATED SOMATIC SITE-SPECIFIC RECOMBINATION IN MICE", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 9, 1997, pages 1766 - 1773, XP001004602, ISSN: 0305-1048 *
ALADJEM MIRIT I ET AL: "Positive of FLP-mediated unequal sister chromatid exchange products in mammalian cells.", MOLECULAR AND CELLULAR BIOLOGY, vol. 17, no. 2, 1997, pages 857 - 861, XP002180200, ISSN: 0270-7306 *
ANGRAND PIERRE-OLIVIER ET AL: "Inducible expression based on regulated recombination: A single vector strategy for stable expression in cultured cells.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 13, 1 July 1998 (1998-07-01), pages 3263 - 3269, XP001021788, ISSN: 0305-1048 *
BOUHASSIRA ERIC E ET AL: "Transcriptional behavior of LCR enhancer elements integrated at the same chromosomal locus by recombinase-mediated cassette exchange.", BLOOD, vol. 90, no. 9, 1997, pages 3332 - 3344, XP002143981, ISSN: 0006-4971 *
ELLEDGE S J ET AL: "LAMBDAYES A MULTIFUNCTIONAL COMPLEMENTARY DNA EXPRESSION VECTOR FOR THE ISOLATION OF GENES BY COMPLEMENTATION OF YEAST AND ESCHERICHIA-COLI MUTATIONS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 88, no. 5, 1991, 1991, pages 1731 - 1735, XP002916290, ISSN: 0027-8424 *
HALL RUTH M ET AL: "Mobile gene cassettes and integrons: Capture and spread of genes by site-specific recombination.", MOLECULAR MICROBIOLOGY, vol. 15, no. 4, 1995, pages 593 - 600, XP001028961, ISSN: 0950-382X *
KILBY N J ET AL: "SITE-SPECIFIC RECOMBINASES: TOOLS FOR GENOME ENGINEERING", TRENDS IN GENETICS, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 9, no. 12, 1993, pages 413 - 421, XP002916291, ISSN: 0168-9525 *
LIU QINGHUA ET AL: "The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes.", CURRENT BIOLOGY, vol. 8, no. 24, 3 December 1998 (1998-12-03), pages 1300 - 1309, XP002155212, ISSN: 0960-9822 *
ODELL J T ET AL: "SEED-SPECIFIC GENE ACTIVATION MEDIATED BY THE CRE/LOX SITE-SPECIFIC RECOMBINATION SYSTEM", PLANT PHYSIOLOGY, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, vol. 106, no. 2, 1 October 1994 (1994-10-01), pages 447 - 458, XP002912196, ISSN: 0032-0889 *
QIN M ET AL: "CRE RECOMBINASE-MEDIATED SITE-SPECIFIC RECOMBINATION BETWEEN PLANT CHROMOSOMES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 91, no. 5, 1 March 1994 (1994-03-01), pages 1706 - 1710, XP000615549, ISSN: 0027-8424 *
SAUER B ET AL: "CONSTRUCTION OF ISOGENIC CELL LINES EXPRESSING HUMAN AND RAT ANGIOTENSIN II AT1 RECEPTORS BY CRE-MEDIATED SITE-SPECIFIC RECOMBINATION", METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 4, no. 2, 1992, pages 143 - 149, XP000613888, ISSN: 1046-2023 *
SCHLAKE THOMAS ET AL: "Use of Mutated FLP Recognition Target (FRT) Sites for the Exchange of Expression Cassettes at Defined Chromosomal Loci.", BIOCHEMISTRY, vol. 33, no. 43, 1994, pages 12746 - 12751, XP000616165, ISSN: 0006-2960 *
See also references of WO0005355A1 *

Also Published As

Publication number Publication date
EP1100885A1 (fr) 2001-05-23
JP2002522013A (ja) 2002-07-23
AU8592298A (en) 2000-02-14
CA2336520A1 (fr) 2000-02-03
WO2000005355A1 (fr) 2000-02-03

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